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9501
[ "LSF", "(", "also", "known", "as", "LBP-1", ",", "UBP", ",", "and", "CP-2", ")", "has", "been", "shown", "to", "repress", "LTR", "transcription", "in", "vitro", ",", "but", "transient", "expression", "of", "LSF", "has", "no", "effect", "on", "LTR", "activity", "in", "vivo", "." ]
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9502
[ "We", "report", "that", "both", "YY1", "and", "LSF", "participate", "in", "the", "formation", "of", "a", "complex", "that", "recognizes", "the", "initiation", "region", "of", "the", "HIV-1", "LTR", "." ]
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9503
[ "Further", ",", "we", "have", "found", "that", "these", "factors", "cooperate", "in", "the", "repression", "of", "LTR", "expression", "and", "viral", "replication", "." ]
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9504
[ "This", "cooperative", "function", "may", "account", "for", "the", "divergent", "effects", "of", "LSF", "previously", "observed", "in", "vitro", "and", "in", "vivo", "." ]
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9505
[ "Thus", ",", "the", "cooperation", "of", "two", "general", "cellular", "transcription", "factors", "may", "allow", "for", "the", "selective", "downregulation", "of", "HIV", "transcription", "." ]
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9506
[ "Through", "this", "mechanism", "of", "gene", "regulation", ",", "YY1", "and", "LSF", "could", "contribute", "to", "the", "establishment", "and", "maintenance", "of", "a", "population", "of", "cells", "stably", "but", "nonproductively", "infected", "with", "HIV-1", "." ]
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9507
[ "Analysis", "of", "interactions", "between", "huGATA-3", "transcription", "factor", "and", "three", "GATA", "regulatory", "elements", "of", "HIV-1", "long", "terminal", "repeat", ",", "by", "surface", "plasmon", "resonance", "." ]
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9508
[ "Relative", "affinities", "of", "transcriptional", "regulatory", "elements", "for", "their", "respective", "factor", "have", "been", "essentially", "studied", "by", "bandshift", "analysis", "." ]
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9509
[ "Here", "we", "report", "a", "real-time", "study", "of", "factor/DNA", "interactions", "using", "a", "surface", "plasmon", "resonance", "approach", "and", "further", "characterization", "of", "recovered", "proteins", "involved", "in", "this", "interaction", "." ]
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9510
[ "For", "this", "purpose", ",", "human", "GATA-3", ",", "either", "recombinant", "or", "in", "nuclear", "extracts", ",", "and", "three", "natural", "GATA", "elements", "of", "the", "HIV-1", "long", "terminal", "repeat", "(", "sites", "1", ",", "2", ",", "and", "3", ")", "were", "chosen", ",", "in", "which", "only", "site", "2", "is", "a", "noncanonical", "GATA", "site", "." ]
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9511
[ "Direct", "analysis", "of", "sensorgrams", ",", "with", "recombinant", "huGATA-3", ",", "allowed", "the", "comparison", "of", "association", "and", "dissociation", "profiles", "of", "the", "three", "DNA", "regions", "and", "their", "ranking", "according", "to", "their", "relative", "affinities", "." ]
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9512
[ "This", "result", ",", "confirmed", "by", "competitions", "with", "each", "GATA", "site", ",", "demonstrated", "the", "higher", "relative", "affinity", "(", "at", "least", "sevenfold", ")", "of", "site", "3", "." ]
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9513
[ "Interactions", "between", "the", "canonical", "and", "unique", "GATA", "site", "3", "and", "nuclear", "extracts", "were", "also", "studied", "in", "real", "time", "and", "provided", "information", "on", "its", "association", "and", "dissociation", "rates", "for", "native", "huGATA-3", "." ]
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9514
[ "Finally", ",", "recovered", "protein", "was", "identified", "as", "genuine", "huGATA-3", "by", "SDS-PAGE", ",", "Western", "blotting", ",", "and", "bandshift", "assays", "." ]
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9515
[ "Copyright", "1997", "Academic", "Press", "." ]
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9516
[ "Stable", "transfection", "of", "U937", "cells", "with", "sense", "or", "antisense", "RXR-alpha", "cDNA", "suggests", "a", "role", "for", "RXR-alpha", "in", "the", "control", "of", "monoblastic", "differentiation", "induced", "by", "retinoic", "acid", "and", "vitamin", "D", "." ]
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9517
[ "Although", "retinoic", "acid", "(", "RA", ")", "has", "been", "known", "for", "many", "years", "to", "be", "a", "modulating", "agent", "that", "plays", "a", "role", "in", "generating", "both", "granulocytes", "and", "monocytes", ",", "the", "molecular", "mechanism", "underlying", "this", "role", "has", "not", "been", "defined", "in", "the", "monoblast", "lineage", "." ]
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9518
[ "In", "particular", ",", "the", "part", "played", "by", "the", "retinoid", "X", "receptors", "(", "RXRs", ")", ",", "which", "are", "members", "of", "the", "steroid/thyroid", "hormone", "nuclear", "receptor", "family", ",", "has", "not", "been", "explored", "." ]
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9519
[ "In", "this", "study", ",", "therefore", ",", "the", "human", "monoblastic", "leukemia", "cell", "line", "U937", "has", "been", "used", "as", "a", "model", "system", "to", "investigate", "the", "role", "of", "one", "of", "the", "RXRs", ",", "RXR-alpha", ",", "in", "monoblast", "differentiation", "." ]
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9520
[ "RXR-alpha", "mRNA", "was", "present", "in", "untreated", "U937", "cells", ",", "and", "levels", "increased", "after", "induction", "of", "differentiation", "with", "phorbol", "ester", "." ]
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9521
[ "The", "same", "was", "found", "for", "RXR-beta", "mRNA", "." ]
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9522
[ "Using", "plasmids", "containing", "sense", "or", "antisense", "RXR-alpha", "sequences", "under", "the", "control", "of", "an", "inducible", "promoter", ",", "we", "generated", "stably", "transfected", "cell", "lines", "which", "expressed", "either", "increased", "or", "decreased", "levels", "of", "RXR-alpha", ",", "respectively", "." ]
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9523
[ "The", "sense", "cell", "lines", "(", "U", "alpha", "S", "and", "its", "clonal", "derivative", "alpha", "G2S", ")", "showed", "increased", "sensitivity", "to", "RA", ",", "while", "the", "antisense", "cell", "lines", "(", "U", "alpha", "A", "and", "its", "clonal", "derivative", "alpha", "B5A", ")", "showed", "decreased", "sensitivity", "to", "RA", ",", "as", "demonstrated", "by", "growth", "inhibition", "and", "by", "regulation", "of", "an", "RA-responsive", "reporter", "gene", "." ]
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9524
[ "Both", "U", "alpha", "A", "and", "alpha", "B5A", "also", "failed", "to", "respond", "to", "another", "modulating", "agent", ",", "1", "alpha", ",", "25-dihydroxycholecalciferol", "(", "DHCC", ")", ",", "but", "only", "U", "alpha", "S", "and", "not", "alpha", "G2S", "showed", "an", "enhanced", "response", "to", "DHCC", "." ]
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9525
[ "The", "combination", "of", "RA", "and", "DHCC", "together", "inhibited", "growth", "of", "both", "sense", "and", "antisense", "cell", "lines", "." ]
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9526
[ "In", "addition", ",", "alpha", "G2S", "exhibited", "increased", "expression", "of", "CD11b", "and", "CD54", ",", "while", "alpha", "B5A", "cells", "showed", "increased", "expression", "of", "CD102", ",", "suggesting", "that", "RXR-alpha", "has", "a", "role", "in", "regulating", "expression", "of", "cell", "adhesion", "molecules", "in", "U937", "cells", "." ]
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9527
[ "These", "results", "demonstrate", "that", "RXR-alpha", "has", "a", "role", "in", "mediating", "growth", "inhibition", "and", "cell", "adhesion", "during", "myelomonocytic", "differentiation", ",", "and", "suggest", "that", "different", "species", "of", "heterodimers", "involving", "RXR-alpha", "may", "control", "the", "acquisition", "of", "different", "features", "of", "mature", "monocyte/macrophage", "function", "." ]
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9528
[ "Transcriptional", "activation", "of", "the", "vascular", "cell", "adhesion", "molecule-1", "gene", "in", "T", "lymphocytes", "expressing", "human", "T-cell", "leukemia", "virus", "type", "1", "Tax", "protein", "." ]
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9529
[ "Recruitment", "and", "extravasation", "of", "T", "cells", "through", "the", "blood-brain", "barrier", "are", "favored", "by", "adhesion", "molecule", "-mediated", "interactions", "of", "circulating", "T", "cells", "with", "endothelial", "cells", "." ]
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9530
[ "Since", "a", "common", "pathological", "finding", "in", "human", "T-cell", "leukemia", "virus", "type", "1", "(", "HTLV-1", ")", "-associated", "diseases", "is", "the", "infiltration", "of", "HTLV-1-infected", "T", "lymphocytes", "into", "various", "organs", ",", "we", "have", "looked", "for", "the", "profile", "of", "adhesion", "molecules", "expressed", "by", "HTLV-1-transformed", "T", "cells", "." ]
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9531
[ "Flow", "cytometry", "analysis", "indicated", "that", "these", "cells", "were", "expressing", "high", "levels", "of", "vascular", "cell", "adhesion", "molecule", "1", "(", "VCAM-1", "[", "CD106", "]", ")", ",", "a", "110-kDa", "member", "of", "the", "immunoglobulin", "gene", "superfamily", ",", "first", "identified", "on", "endothelial", "cells", "stimulated", "with", "inflammatory", "cytokines", "." ]
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9532
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9533
[ "The", "role", "of", "the", "viral", "trans-activator", "Tax", "protein", "in", "the", "induction", "of", "VCAM-1", "was", "first", "indicated", "by", "the", "detection", "of", "this", "adhesion", "molecule", "on", "Jurkat", "T-cell", "clones", "stably", "expressing", "the", "tax", "gene", "." ]
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9534
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9535
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9536
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9537
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9538
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9539
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9540
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9541
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9542
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9543
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9544
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9545
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9546
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9547
[ "Tissue", "transglutaminase-dependent", "posttranslational", "modification", "of", "the", "retinoblastoma", "gene", "product", "in", "promonocytic", "cells", "undergoing", "apoptosis", "." ]
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9548
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9549
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9550
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