siddharthtumre/Revised-JNLPBA · Datasets at Hugging Face

id stringlengths 1 5	tokens sequence	ner_tags sequence
8101	[ "These", "results", "support", "our", "hypothesis", "that", "HCMV", "initiates", "a", "signal", "transduction", "pathway", "that", "leads", "to", "monocyte", "activation", "and", "pinpoints", "a", "potential", "mechanism", "whereby", "HCMV", "infection", "of", "monocytes", "can", "result", "in", "profound", "pathogenesis", ",", "especially", "in", "chronic", "inflammatory-type", "conditions", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8102	[ "Regulation", "of", "the", "megakaryocytic", "glycoprotein", "IX", "promoter", "by", "the", "oncogenic", "Ets", "transcription", "factor", "Fli-1", "." ]	[ 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 9, 10, 10, 10, 0 ]
8103	[ "Glycoprotein", "(", "GP", ")", "IX", "is", "a", "subunit", "of", "the", "von", "Willebrand", "receptor", ",", "GPIb-V-IX", ",", "which", "mediates", "adhesion", "of", "platelets", "to", "the", "subendothelium", "of", "damaged", "blood", "vessels", "." ]	[ 9, 10, 10, 10, 10, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0 ]
8104	[ "Previous", "characterization", "of", "the", "GPIX", "promoter", "identified", "a", "functional", "Ets", "site", "that", ",", "when", "disrupted", ",", "reduced", "promoter", "activity", "." ]	[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8105	[ "However", ",", "the", "Ets", "protein", "(", "s", ")", "that", "regulated", "GPIX", "promoter", "expression", "was", "unknown", "." ]	[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0 ]
8106	[ "In", "this", "study", ",", "transient", "cotransfection", "of", "several", "GPIX", "promoter/reporter", "constructs", "into", "293T", "kidney", "fibroblasts", "with", "a", "Fli-1", "expression", "vector", "shows", "that", "the", "oncogenic", "protein", "Fli-1", "can", "transactivate", "the", "GPIX", "promoter", "when", "an", "intact", "GPIX", "Ets", "site", "is", "present", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 5, 6, 6, 0, 0, 1, 2, 2, 0, 0, 0, 0, 9, 10, 0, 0, 0, 1, 2, 0, 0, 0, 1, 2, 2, 0, 0, 0 ]
8107	[ "In", "addition", ",", "Fli-1", "binding", "of", "the", "GPIX", "Ets", "site", "was", "identified", "in", "antibody", "supershift", "experiments", "in", "nuclear", "extracts", "derived", "from", "hematopoietic", "human", "erythroleukemia", "cells", "." ]	[ 0, 0, 0, 9, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0 ]
8108	[ "Comparative", "studies", "showed", "that", "Fli-1", "was", "also", "able", "to", "transactivate", "the", "GPIbalpha", "and", ",", "to", "a", "lesser", "extent", ",", "the", "GPIIb", "promoter", "." ]	[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]
8109	[ "Immunoblot", "analysis", "identified", "Fli-1", "protein", "in", "lysates", "derived", "from", "platelets", "." ]	[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 7, 0 ]
8110	[ "In", "addition", ",", "expression", "of", "Fli-1", "was", "identified", "immunohistochemically", "in", "megakaryocytes", "derived", "from", "CD34", "(", "+", ")", "cells", "treated", "with", "the", "megakaryocyte", "differentiation", "and", "proliferation", "factor", ",", "thrombopoietin", "." ]	[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 9, 0 ]
8111	[ "These", "results", "suggest", "that", "Fli-1", "is", "likely", "to", "regulate", "lineage-specific", "genes", "during", "megakaryocytopoiesis", "." ]	[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8112	[ "Expression", "of", "IkappaBalpha", "in", "the", "nucleus", "of", "human", "peripheral", "blood", "T", "lymphocytes", "." ]	[ 0, 0, 9, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0 ]
8113	[ "According", "to", "current", "models", "the", "inhibitory", "capacity", "of", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "would", "be", "mediated", "through", "the", "retention", "of", "Rel/NF-kappaB", "proteins", "in", "the", "cytosol", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0 ]
8114	[ "However", ",", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "has", "also", "been", "detected", "in", "the", "nucleus", "of", "cell", "lines", "and", "when", "overexpressed", "by", "transient", "transfection", "." ]	[ 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8115	[ "To", "gain", "better", "insight", "into", "the", "potential", "role", "of", "nuclear", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "in", "a", "physiological", "context", "we", "have", "analysed", "its", "presence", "in", "the", "nucleus", "of", "human", "peripheral", "blood", "T", "lymphocytes", "(", "PBL", ")", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 7, 0, 0 ]
8116	[ "We", "demonstrate", "the", "nuclear", "localization", "of", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "in", "PBL", "by", "different", "techniques", ":", "Western", "blot", ",", "indirect", "immunofluorescence", "and", "electron", "microscopy", "." ]	[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8117	[ "Low", "levels", "of", "nuclear", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "were", "detected", "in", "resting", "cells", "whereas", "a", "superinduction", "was", "obtained", "after", "PMA", "activation", "." ]	[ 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8118	[ "The", "nuclear", "pool", "of", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "showed", "a", "higher", "stability", "than", "cytosolic", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "and", "was", "partially", "independent", "of", "the", "resynthesis", "of", "the", "protein", "." ]	[ 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8119	[ "Unexpectedly", ",", "the", "presence", "of", "nuclear", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "did", "not", "inhibit", "NF-kappaB", "binding", "to", "DNA", "and", "this", "phenomenon", "was", "not", "due", "to", "the", "presence", "of", "IkappaBbeta", "at", "the", "nuclear", "level", "." ]	[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0 ]
8120	[ "Immunoprecipitation", "experiments", "failed", "to", "demonstrate", "an", "association", "between", "nuclear", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "and", "NF-kappaB", "proteins", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 9, 0, 0 ]
8121	[ "Our", "results", "demonstrate", "that", "in", "resting", "and", "PMA-activated", "human", "PBL", ",", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "is", "present", "in", "the", "nucleus", "in", "an", "apparently", "inactive", "form", "unable", "to", "disrupt", "NF-kappaB", "binding", "from", "DNA", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]
8122	[ "Impaired", "fetal", "thymocyte", "development", "after", "efficient", "adenovirus-mediated", "inhibition", "of", "NF-kappa", "B", "activation", "." ]	[ 0, 7, 8, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]
8123	[ "We", "introduce", "a", "new", "experimental", "system", "combining", "adenovirus-mediated", "gene", "transfer", "and", "fetal", "thymic", "organ", "culture", "(", "FTOC", ")", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8124	[ "This", "system", "allowed", "us", "to", "efficiently", "express", "in", "developing", "thymocytes", "a", "mutant", "form", "of", "the", "NF-kappa", "B", "inhibitor", "I", "kappa", "B", "alpha", "(", "mut-I", "kappa", "B", ")", "and", "to", "study", "the", "maturation", "defects", "occurring", "when", "NF-kappa", "B", "activation", "is", "inhibited", "during", "fetal", "development", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 10, 10, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0 ]
8125	[ "Fetal", "thymocytes", "infected", "with", "adenovirus", "containing", "mut-I", "kappa", "B", "were", "found", "to", "develop", "normally", "until", "the", "CD44", "-CD25+", ",", "CD4", "-", "CD8", "-", "double-negative", "stage", ",", "while", "production", "of", "more", "mature", "double-positive", "and", "single-positive", "populations", "was", "strongly", "decreased", "." ]	[ 7, 8, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8126	[ "Proliferation", ",", "as", "measured", "by", "the", "percentage", "of", "cells", "in", "cycle", "appeared", "normal", ",", "as", "did", "rearrangement", "and", "expression", "of", "the", "TCR", "beta-chain", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]
8127	[ "However", ",", "apoptosis", "was", "much", "higher", "in", "FTOC", "infected", "with", "adenovirus", "containing", "mut-I", "kappa", "B", "than", "in", "FTOC", "infected", "with", "a", "control", "virus", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8128	[ "Taken", "together", ",", "these", "results", "suggest", "that", "NF-kappa", "B", "plays", "a", "crucial", "role", "in", "ensuring", "the", "differentiation", "and", "survival", "of", "thymocytes", "in", "the", "early", "stages", "of", "their", "development", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0 ]
8129	[ "The", "relationship", "between", "Ca2+-ATPase", "and", "freely", "exchangeable", "Ca2+", "in", "the", "dense", "tubules", ":", "a", "study", "in", "platelets", "from", "women", "." ]	[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0 ]
8130	[ "The", "main", "aims", "of", "this", "work", "were", "to", "examine", "in", "women", ":", "the", "relationship", "between", "the", "freely", "exchangeable", "Ca2+", "(", "FECa2+", ")", "in", "the", "dense", "tubules", "and", "the", "activity", "of", "the", "sarco", "(", "endo", ")", "plasmic", "reticulum", "(", "SER", ")", "Ca2+-ATPase", "(", "SERCA", ")", "in", "platelets", ",", "and", "the", "relationship", "of", "these", "parameters", "with", "blood", "pressure", "and", "serum", "lipoproteins", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0, 9, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8131	[ "Platelets", "from", "14", "white", "and", "13", "black", "women", "in", "good", "health", "were", "studied", "." ]	[ 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8132	[ "The", "FECa2+", "was", "measured", "as", "the", "ionomycin-evoked", "Ca2+", "release", "(", "in", "the", "presence", "of", "thapsigargin", ")", "in", "Ca2+-free", "medium", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8133	[ "SERCA", "activity", "was", "measured", "as", "the", "thapsigargin", "sensitive", ",", "Ca2+", "dependent", "and", "ouabain", "resistant", ",", "ATP", "hydrolyses", "in", "platelet", "membranes", "." ]	[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0 ]
8134	[ "Relative", "expressions", "of", "SERCA", "2", "and", "3", "isoforms", "and", "Ras-related", "protein", "(", "Rap", ")", "1", "in", "platelet", "membranes", "were", "determined", "by", "Western", "immunoblots", "." ]	[ 0, 0, 0, 9, 10, 0, 9, 10, 0, 9, 10, 10, 10, 10, 10, 0, 7, 0, 0, 0, 0, 0, 0, 0 ]
8135	[ "Highly", "significant", "correlations", "were", "observed", "for", "FECa2+", "in", "the", "dense", "tubules", "with", ":", "1", ")", "the", "maximal", "reaction", "velocity", "(", "Vmax", ")", "of", "the", "SERCA", "(", "r", "=", "0.592", ",", "P", "=", ".0014", ")", ",", "and", "2", ")", "Rapl", "(", "r", "=", "0.551", ",", "P", "=", ".0035", ")", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8136	[ "In", "addition", ",", "negative", "correlations", "were", "observed", "between", "FECa2+", "in", "the", "dense", "tubules", "and", "age", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8137	[ "No", "correlations", "were", "observed", "for", "these", "variables", "with", "blood", "pressure", "or", "serum", "lipoproteins", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8138	[ "We", "conclude", "the", "FECa2+", "and", "the", "Vmax", "of", "the", "SERCA", "are", "reliable", "indicators", "of", "Ca2+", "load", "in", "platelets", "from", "women", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0 ]
8139	[ "However", ",", "in", "women", ",", "unlike", "previous", "observations", "in", "men", ",", "these", "platelet", "parameters", "are", "not", "correlated", "with", "blood", "pressure", "and", "serum", "lipoproteins", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8140	[ "Cobalt", "chloride-induced", "signaling", "in", "endothelium", "leading", "to", "the", "augmented", "adherence", "of", "sickle", "red", "blood", "cells", "and", "transendothelial", "migration", "of", "monocyte-like", "HL-60", "cells", "is", "blocked", "by", "PAF-receptor", "antagonist", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0 ]
8141	[ "In", "response", "to", "hypoxia", ",", "sickle", "red", "blood", "cells", "(", "SS", "RBC", ")", "and", "leukocytes", "exhibit", "increased", "adherence", "to", "the", "vascular", "endothelium", ",", "while", "diapedesis", "of", "leukocytes", "through", "the", "blood", "vessel", "increases", "." ]	[ 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 7, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0 ]
8142	[ "However", ",", "the", "cellular", "signaling", "pathway", "(", "s", ")", "caused", "by", "hypoxia", "is", "poorly", "understood", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8143	[ "We", "utilized", "CoCl2", "as", "a", "mimetic", "molecule", "for", "hypoxia", "to", "study", "cellular", "signaling", "pathways", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8144	[ "We", "found", "that", "in", "human", "umbilical", "vein", "endothelial", "cells", "(", "HUVEC", ")", ",", "CoCl2", "at", "2", "mM", "concentration", "induced", "the", "surface", "expression", "of", "a", "subset", "of", "CAMs", "(", "VCAM-1", ")", "and", "activation", "of", "transcription", "factor", "NF-kappaB", "in", "the", "nuclear", "extracts", "of", "HUVEC", "." ]	[ 0, 0, 0, 0, 7, 8, 8, 8, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 7, 0 ]
8145	[ "Furthermore", ",", "CoCl2", "also", "caused", "time-dependent", "tyrosine", "phosphorylation", "of", "mitogen-activated", "protein", "(", "MAP", ")", "kinase", "isoform", "ERK2", "without", "significantly", "affecting", "ERK1", ",", "indicating", "ERK2", "is", "the", "preferred", "substrate", "for", "upstream", "kinase", "of", "the", "MAPK", "pathway", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0, 9, 0, 0, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]
8146	[ "Inhibitors", "of", "MAP", "kinase", "(", "PD98059", ")", "or", "platelet-activating", "factor", "(", "PAF", ")", "-", "receptor", "antagonist", "(", "CV3988", ")", "inhibited", "the", "CoCl2-induced", "NF-kappaB", "activation", "and", "VCAM-1", "expression", "." ]	[ 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0, 0 ]
8147	[ "Augmented", "expression", "of", "VCAM-1", "led", "to", "increased", "SS", "RBC", "adhesion", ",", "inhibitable", "by", "a", "VCAM-1", "antibody", "." ]	[ 0, 0, 0, 9, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 9, 10, 0 ]
8148	[ "Additionally", ",", "CoCl2", "caused", "a", "two-", "to", "threefold", "increase", "in", "the", "rate", "of", "transendothelial", "migration", "of", "monocyte-like", "HL-60", "cells", "and", "a", "twentyfold", "increase", "in", "phosphorylation", "of", "platelet", "endothelial", "cell", "adhesion", "molecules", "(", "PECAM-1", ")", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 0, 0 ]
8149	[ "The", "transendothelial", "migration", "of", "monocytes", "was", "inhibited", "by", "an", "antibody", "to", "PECAM-1", "." ]	[ 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 9, 0 ]
8150	[ "Both", "phosphorylation", "of", "PECAM-1", "and", "transendothelial", "migration", "of", "monocytes", "in", "response", "to", "CoCl2", "were", "inhibited", "by", "protein", "kinase", "inhibitor", "(", "GF109203X", ")", "and", "augmented", "by", "protein", "phosphatase", "inhibitor", "(", "Calyculin", "A", ")", "." ]	[ 0, 0, 0, 9, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0 ]
8151	[ "Our", "data", "suggests", "that", "CoCl2-induced", "cellular", "signals", "directing", "increased", "expression", "of", "VCAM-1", "in", "HUVEC", "involve", "downstream", "activation", "of", "MAP", "kinase", "and", "NF-kappaB", ",", "while", "the", "phosphorylation", "of", "PECAM-1", "occurs", "as", "a", "result", "of", "activation", "of", "PKC", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 7, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
8152	[ "We", "conclude", "that", "PAF-receptor", "antagonist", "inhibits", "the", "CoCl2", "-or", "hypoxia-induced", "increase", "in", "the", "adhesion", "of", "SS", "RBC", ",", "PECAM-1", "phosphorylation", ",", "and", "the", "concomitant", "transendothelial", "migration", "of", "monocytes", "." ]	[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0 ]
8153	[ "Bacterial", "lipopolysaccharide", "activates", "nuclear", "factor-kappaB", "through", "interleukin-1", "signaling", "mediators", "in", "cultured", "human", "dermal", "endothelial", "cells", "and", "mononuclear", "phagocytes", "." ]	[ 0, 0, 0, 9, 10, 0, 9, 10, 10, 0, 0, 7, 8, 8, 8, 0, 7, 8, 0 ]
8154	[ "Bacterial", "lipopolysaccharide", "(", "LPS", ")", "-mediated", "immune", "responses", ",", "including", "activation", "of", "monocytes", ",", "macrophages", ",", "and", "endothelial", "cells", ",", "play", "an", "important", "role", "in", "the", "pathogenesis", "of", "Gram-negative", "bacteria-induced", "sepsis", "syndrome", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 7, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
8155	[ "Activation", "of", "NF-kappaB", "is", "thought", "to", "be", "required", "for", "cytokine", "release", "from", "LPS-responsive", "cells", ",", "a", "critical", "step", "for", "endotoxic", "effects", "." ]	[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
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8157	[ "LPS", "stimulation", "of", "HDMEC", "and", "THP-1", "cells", "initiated", "an", "IL-1", "receptor", "-like", "NF-kappaB", "signaling", "cascade", "." ]	[ 0, 0, 0, 7, 0, 5, 6, 0, 0, 9, 10, 0, 9, 0, 0, 0 ]
8158	[ "In", "transient", "cotransfection", "experiments", ",", "dominant", "negative", "mutants", "of", "the", "IL-1", "signaling", "pathway", ",", "including", "MyD88", ",", "IRAK", ",", "IRAK2", ",", "and", "TRAF6", "inhibited", "both", "IL-1", "-and", "LPS-induced", "NF-kappaB", "-luciferase", "activity", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 9, 0, 9, 0, 0, 9, 0, 0, 9, 0, 0, 9, 10, 0, 0 ]
8159	[ "LPS-induced", "NF-kappaB", "activation", "was", "not", "inhibited", "by", "a", "dominant", "negative", "mutant", "of", "TRAF2", "that", "is", "involved", "in", "TNF", "signaling", "." ]	[ 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 0 ]
8160	[ "LPS-induced", "activation", "of", "NF-kappaB-responsive", "reporter", "gene", "was", "not", "inhibited", "by", "IL-1", "receptor", "antagonist", "." ]	[ 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 9, 10, 0, 0 ]
8161	[ "TLR2", "and", "TLR4", "were", "expressed", "on", "the", "cell", "surface", "of", "HDMEC", "and", "THP-1", "cells", "." ]	[ 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 7, 0, 5, 6, 0 ]
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8163	[ "Signal", "transduction", "through", "interferon-gamma", "receptor", "on", "human", "eosinophils", "." ]	[ 0, 0, 0, 9, 10, 0, 7, 8, 0 ]
8164	[ "BACKGROUND", ":", "We", "reported", "on", "the", "constitutive", "interferon-gamma", "receptor", "(", "IFN-gammaR", ")", "expression", "on", "eosinophils", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 7, 0 ]
8165	[ "But", "signal", "transduction", "through", "IFN-gammaR", "on", "eosinophils", "remains", "to", "be", "elucidated", "." ]	[ 0, 0, 0, 0, 9, 0, 7, 0, 0, 0, 0, 0 ]
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8167	[ "METHODS", ":", "Purified", "peripheral", "eosinophils", "were", "stimulated", "with", "IFN-gamma", "at", "37", "degrees", "C", "for", "1-60", "min", "." ]	[ 0, 0, 0, 7, 8, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]
8168	[ "Tyrosine", "phosphorylation", "of", "IFN-gammaR", ",", "Jak1", ",", "Jak2", ",", "and", "Stat1alpha", "was", "examined", "by", "immunoblotting", "." ]	[ 0, 0, 0, 9, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0 ]
8169	[ "Gel-shift", "assay", "was", "also", "examined", "to", "show", "the", "formation", "of", "Stat1alpha-DNA", "complexes", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
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8171	[ "In", "addition", ",", "IFN-gamma", "enhanced", "the", "DNA-binding", "activity", "of", "Stat1alpha", "." ]	[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0 ]
8172	[ "CONCLUSION", ":", "These", "data", "indicate", "that", "IFN-gamma", "affects", "eosinophils", "through", "its", "specific", "receptor", "and", "utilizes", "the", "Jak/Stat", "pathway", "as", "its", "mode", "of", "signaling", "." ]	[ 0, 0, 0, 0, 0, 0, 9, 0, 7, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]
8173	[ "Expression", "of", "E2A-HLF", "chimeric", "protein", "induced", "T-cell", "apoptosis", ",", "B-cell", "maturation", "arrest", ",", "and", "development", "of", "acute", "lymphoblastic", "leukemia", "." ]	[ 0, 0, 9, 10, 10, 0, 7, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
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8180	[ "Moreover", ",", "several", "transgenic", "mice", "developed", "acute", "leukemia", ",", "classified", "as", "T-ALL", "based", "on", "the", "surface", "marker", "analysis", "and", "DNA", "rearrangements", ",", "suggesting", "that", "an", "additional", "event", "is", "required", "for", "malignant", "transformation", "of", "lymphoid", "cells", "expressing", "E2A-HLF", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 9, 0 ]
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8184	[ "RA", "receptor", "activation", "appears", "critical", "to", "regulating", "important", "aspects", "of", "hematopoiesis", ",", "since", "transducing", "a", "COOH-terminally", "truncated", "RARalpha", "exhibiting", "dominant-negative", "activity", "(", "RARalpha403", ")", "into", "normal", "mouse", "bone", "marrow", "generates", "hematopoietic", "growth", "factor-dependent", "cell", "lines", "frozen", "at", "the", "multipotent", "progenitor", "(", "EML", ")", "or", "committed", "promyelocyte", "(", "MPRO", ")", "stages", "." ]	[ 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 7, 0, 5, 0, 0, 0 ]
8185	[ "Nevertheless", ",", "relatively", "high", ",", "pharmacological", "concentrations", "of", "RA", "(", "1", "to", "10", "&", "mgr", ";", "M", ")", "overcome", "these", "differentiation", "blocks", "and", "induce", "terminal", "granulocytic", "differentiation", "of", "the", "MPRO", "promyelocytes", "while", "potentiating", "interleukin-3", "(", "IL-3", ")", "-induced", "commitment", "of", "EML", "cells", "to", "the", "granulocyte/monocyte", "lineage", "." ]	[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 9, 0, 9, 0, 0, 0, 0, 5, 6, 0, 0, 7, 8, 0 ]
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8101

[ "These", "results", "support", "our", "hypothesis", "that", "HCMV", "initiates", "a", "signal", "transduction", "pathway", "that", "leads", "to", "monocyte", "activation", "and", "pinpoints", "a", "potential", "mechanism", "whereby", "HCMV", "infection", "of", "monocytes", "can", "result", "in", "profound", "pathogenesis", ",", "especially", "in", "chronic", "inflammatory-type", "conditions", "." ]

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8102

[ "Regulation", "of", "the", "megakaryocytic", "glycoprotein", "IX", "promoter", "by", "the", "oncogenic", "Ets", "transcription", "factor", "Fli-1", "." ]

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8103

[ "Glycoprotein", "(", "GP", ")", "IX", "is", "a", "subunit", "of", "the", "von", "Willebrand", "receptor", ",", "GPIb-V-IX", ",", "which", "mediates", "adhesion", "of", "platelets", "to", "the", "subendothelium", "of", "damaged", "blood", "vessels", "." ]

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8104

[ "Previous", "characterization", "of", "the", "GPIX", "promoter", "identified", "a", "functional", "Ets", "site", "that", ",", "when", "disrupted", ",", "reduced", "promoter", "activity", "." ]

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8105

[ "However", ",", "the", "Ets", "protein", "(", "s", ")", "that", "regulated", "GPIX", "promoter", "expression", "was", "unknown", "." ]

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8106

[ "In", "this", "study", ",", "transient", "cotransfection", "of", "several", "GPIX", "promoter/reporter", "constructs", "into", "293T", "kidney", "fibroblasts", "with", "a", "Fli-1", "expression", "vector", "shows", "that", "the", "oncogenic", "protein", "Fli-1", "can", "transactivate", "the", "GPIX", "promoter", "when", "an", "intact", "GPIX", "Ets", "site", "is", "present", "." ]

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8107

[ "In", "addition", ",", "Fli-1", "binding", "of", "the", "GPIX", "Ets", "site", "was", "identified", "in", "antibody", "supershift", "experiments", "in", "nuclear", "extracts", "derived", "from", "hematopoietic", "human", "erythroleukemia", "cells", "." ]

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8108

[ "Comparative", "studies", "showed", "that", "Fli-1", "was", "also", "able", "to", "transactivate", "the", "GPIbalpha", "and", ",", "to", "a", "lesser", "extent", ",", "the", "GPIIb", "promoter", "." ]

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8109

[ "Immunoblot", "analysis", "identified", "Fli-1", "protein", "in", "lysates", "derived", "from", "platelets", "." ]

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8110

[ "In", "addition", ",", "expression", "of", "Fli-1", "was", "identified", "immunohistochemically", "in", "megakaryocytes", "derived", "from", "CD34", "(", "+", ")", "cells", "treated", "with", "the", "megakaryocyte", "differentiation", "and", "proliferation", "factor", ",", "thrombopoietin", "." ]

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8111

[ "These", "results", "suggest", "that", "Fli-1", "is", "likely", "to", "regulate", "lineage-specific", "genes", "during", "megakaryocytopoiesis", "." ]

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8112

[ "Expression", "of", "IkappaBalpha", "in", "the", "nucleus", "of", "human", "peripheral", "blood", "T", "lymphocytes", "." ]

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8113

[ "According", "to", "current", "models", "the", "inhibitory", "capacity", "of", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "would", "be", "mediated", "through", "the", "retention", "of", "Rel/NF-kappaB", "proteins", "in", "the", "cytosol", "." ]

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8114

[ "However", ",", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "has", "also", "been", "detected", "in", "the", "nucleus", "of", "cell", "lines", "and", "when", "overexpressed", "by", "transient", "transfection", "." ]

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8115

[ "To", "gain", "better", "insight", "into", "the", "potential", "role", "of", "nuclear", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "in", "a", "physiological", "context", "we", "have", "analysed", "its", "presence", "in", "the", "nucleus", "of", "human", "peripheral", "blood", "T", "lymphocytes", "(", "PBL", ")", "." ]

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8116

[ "We", "demonstrate", "the", "nuclear", "localization", "of", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "in", "PBL", "by", "different", "techniques", ":", "Western", "blot", ",", "indirect", "immunofluorescence", "and", "electron", "microscopy", "." ]

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8117

[ "Low", "levels", "of", "nuclear", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "were", "detected", "in", "resting", "cells", "whereas", "a", "superinduction", "was", "obtained", "after", "PMA", "activation", "." ]

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8118

[ "The", "nuclear", "pool", "of", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "showed", "a", "higher", "stability", "than", "cytosolic", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "and", "was", "partially", "independent", "of", "the", "resynthesis", "of", "the", "protein", "." ]

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8119

[ "Unexpectedly", ",", "the", "presence", "of", "nuclear", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "did", "not", "inhibit", "NF-kappaB", "binding", "to", "DNA", "and", "this", "phenomenon", "was", "not", "due", "to", "the", "presence", "of", "IkappaBbeta", "at", "the", "nuclear", "level", "." ]

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8120

[ "Immunoprecipitation", "experiments", "failed", "to", "demonstrate", "an", "association", "between", "nuclear", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "and", "NF-kappaB", "proteins", "." ]

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8121

[ "Our", "results", "demonstrate", "that", "in", "resting", "and", "PMA-activated", "human", "PBL", ",", "I", "(", "kappa", ")", "B", "(", "alpha", ")", "is", "present", "in", "the", "nucleus", "in", "an", "apparently", "inactive", "form", "unable", "to", "disrupt", "NF-kappaB", "binding", "from", "DNA", "." ]

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8122

[ "Impaired", "fetal", "thymocyte", "development", "after", "efficient", "adenovirus-mediated", "inhibition", "of", "NF-kappa", "B", "activation", "." ]

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8123

[ "We", "introduce", "a", "new", "experimental", "system", "combining", "adenovirus-mediated", "gene", "transfer", "and", "fetal", "thymic", "organ", "culture", "(", "FTOC", ")", "." ]

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8124

[ "This", "system", "allowed", "us", "to", "efficiently", "express", "in", "developing", "thymocytes", "a", "mutant", "form", "of", "the", "NF-kappa", "B", "inhibitor", "I", "kappa", "B", "alpha", "(", "mut-I", "kappa", "B", ")", "and", "to", "study", "the", "maturation", "defects", "occurring", "when", "NF-kappa", "B", "activation", "is", "inhibited", "during", "fetal", "development", "." ]

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8125

[ "Fetal", "thymocytes", "infected", "with", "adenovirus", "containing", "mut-I", "kappa", "B", "were", "found", "to", "develop", "normally", "until", "the", "CD44", "-CD25+", ",", "CD4", "-", "CD8", "-", "double-negative", "stage", ",", "while", "production", "of", "more", "mature", "double-positive", "and", "single-positive", "populations", "was", "strongly", "decreased", "." ]

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8126

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8127

[ "However", ",", "apoptosis", "was", "much", "higher", "in", "FTOC", "infected", "with", "adenovirus", "containing", "mut-I", "kappa", "B", "than", "in", "FTOC", "infected", "with", "a", "control", "virus", "." ]

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8128

[ "Taken", "together", ",", "these", "results", "suggest", "that", "NF-kappa", "B", "plays", "a", "crucial", "role", "in", "ensuring", "the", "differentiation", "and", "survival", "of", "thymocytes", "in", "the", "early", "stages", "of", "their", "development", "." ]

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8129

[ "The", "relationship", "between", "Ca2+-ATPase", "and", "freely", "exchangeable", "Ca2+", "in", "the", "dense", "tubules", ":", "a", "study", "in", "platelets", "from", "women", "." ]

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8130

[ "The", "main", "aims", "of", "this", "work", "were", "to", "examine", "in", "women", ":", "the", "relationship", "between", "the", "freely", "exchangeable", "Ca2+", "(", "FECa2+", ")", "in", "the", "dense", "tubules", "and", "the", "activity", "of", "the", "sarco", "(", "endo", ")", "plasmic", "reticulum", "(", "SER", ")", "Ca2+-ATPase", "(", "SERCA", ")", "in", "platelets", ",", "and", "the", "relationship", "of", "these", "parameters", "with", "blood", "pressure", "and", "serum", "lipoproteins", "." ]

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8131

[ "Platelets", "from", "14", "white", "and", "13", "black", "women", "in", "good", "health", "were", "studied", "." ]

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8132

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8133

[ "SERCA", "activity", "was", "measured", "as", "the", "thapsigargin", "sensitive", ",", "Ca2+", "dependent", "and", "ouabain", "resistant", ",", "ATP", "hydrolyses", "in", "platelet", "membranes", "." ]

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8134

[ "Relative", "expressions", "of", "SERCA", "2", "and", "3", "isoforms", "and", "Ras-related", "protein", "(", "Rap", ")", "1", "in", "platelet", "membranes", "were", "determined", "by", "Western", "immunoblots", "." ]

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8135

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8136

[ "In", "addition", ",", "negative", "correlations", "were", "observed", "between", "FECa2+", "in", "the", "dense", "tubules", "and", "age", "." ]

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8137

[ "No", "correlations", "were", "observed", "for", "these", "variables", "with", "blood", "pressure", "or", "serum", "lipoproteins", "." ]

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8138

[ "We", "conclude", "the", "FECa2+", "and", "the", "Vmax", "of", "the", "SERCA", "are", "reliable", "indicators", "of", "Ca2+", "load", "in", "platelets", "from", "women", "." ]

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8139

[ "However", ",", "in", "women", ",", "unlike", "previous", "observations", "in", "men", ",", "these", "platelet", "parameters", "are", "not", "correlated", "with", "blood", "pressure", "and", "serum", "lipoproteins", "." ]

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8140

[ "Cobalt", "chloride-induced", "signaling", "in", "endothelium", "leading", "to", "the", "augmented", "adherence", "of", "sickle", "red", "blood", "cells", "and", "transendothelial", "migration", "of", "monocyte-like", "HL-60", "cells", "is", "blocked", "by", "PAF-receptor", "antagonist", "." ]

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8141

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8142

[ "However", ",", "the", "cellular", "signaling", "pathway", "(", "s", ")", "caused", "by", "hypoxia", "is", "poorly", "understood", "." ]

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8143

[ "We", "utilized", "CoCl2", "as", "a", "mimetic", "molecule", "for", "hypoxia", "to", "study", "cellular", "signaling", "pathways", "." ]

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8144

[ "We", "found", "that", "in", "human", "umbilical", "vein", "endothelial", "cells", "(", "HUVEC", ")", ",", "CoCl2", "at", "2", "mM", "concentration", "induced", "the", "surface", "expression", "of", "a", "subset", "of", "CAMs", "(", "VCAM-1", ")", "and", "activation", "of", "transcription", "factor", "NF-kappaB", "in", "the", "nuclear", "extracts", "of", "HUVEC", "." ]

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8145

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8146

[ "Inhibitors", "of", "MAP", "kinase", "(", "PD98059", ")", "or", "platelet-activating", "factor", "(", "PAF", ")", "-", "receptor", "antagonist", "(", "CV3988", ")", "inhibited", "the", "CoCl2-induced", "NF-kappaB", "activation", "and", "VCAM-1", "expression", "." ]

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8147

[ "Augmented", "expression", "of", "VCAM-1", "led", "to", "increased", "SS", "RBC", "adhesion", ",", "inhibitable", "by", "a", "VCAM-1", "antibody", "." ]

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8148

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8149

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8150

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8151

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8152

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8153

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8154

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8155

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8156

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8157

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8158

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8159

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8160

[ "LPS-induced", "activation", "of", "NF-kappaB-responsive", "reporter", "gene", "was", "not", "inhibited", "by", "IL-1", "receptor", "antagonist", "." ]

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8161

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8162

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8163

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8164

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8165

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8166

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8167

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8168

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8169

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8170

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8171

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8172

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8173

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8174

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8175

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8176

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8177

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8178

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8179

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8180

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8181

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8182

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8183

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8184

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8185

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8186

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8187

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8188

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8189

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8190

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8191

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8192

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8193

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8194

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8195

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8196

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8197

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