Datasets:
siddharthtumre
/
Revised-JNLPBA

Dataset card Data Studio Files Community
1
id
stringlengths
1
5
tokens
sequence
ner_tags
sequence
7701
[ "Here", "we", "show", "that", "fludarabine", ",", "but", "not", "the", "immunosuppressant", "cyclosporine", "A", ",", "inhibits", "the", "cytokine", "-induced", "activation", "of", "STAT1", "and", "STAT1", "-dependent", "gene", "transcription", "in", "normal", "resting", "or", "activated", "lymphocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 7, 0 ]
7702
[ "Fludarabine", "caused", "a", "specific", "depletion", "of", "STAT1", "protein", "(", "and", "mRNA", ")", "but", "not", "of", "other", "STATs", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
7703
[ "This", "loss", "of", "STAT1", "was", "also", "seen", "in", "cells", "from", "patients", "treated", "with", "fludarabine", "in", "vivo", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7704
[ "Brief", "exposure", "to", "fludarabine", "led", "to", "a", "sustained", "loss", "of", "STAT1", ",", "analogous", "to", "the", "prolonged", "period", "of", "immunosuppression", "induced", "by", "exposure", "to", "the", "drug", "in", "vivo", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7705
[ "Thus", ",", "STAT1", "may", "be", "a", "useful", "target", "in", "the", "development", "of", "new", "immunosuppressive", "and", "antineoplastic", "agents", "." ]
[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7706
[ "Studies", "into", "the", "effect", "of", "tyrosine", "phosphatase", "inhibitor", "phenylarsine", "oxide", "on", "NFkappaB", "activation", "in", "T", "lymphocytes", "during", "aging", ":", "evidence", "for", "altered", "IkappaB-alpha", "phosphorylation", "and", "degradation", "." ]
[ 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]
7707
[ "Nuclear", "Factor", "kappa", "B", "(", "NFkappaB", ")", "is", "a", "critical", "regulator", "of", "several", "genes", "involved", "in", "immune", "and", "inflammatory", "responses", "." ]
[ 9, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7708
[ "Treatment", "of", "T", "cells", "with", "a", "variety", "of", "stimuli", ",", "including", "TNF-alpha", ",", "leads", "to", "the", "translocation", "of", "the", "active", "p65-50", "heterodimer", "to", "the", "nucleus", ",", "albeit", "at", "a", "lower", "level", "in", "T", "cells", "from", "the", "elderly", "." ]
[ 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0 ]
7709
[ "We", "demonstrate", "here", "that", "pretreatment", "with", "PAO", "results", "in", "the", "inhibition", "of", "NFkappaB", "induction", "in", "TNF-alpha", "treated", "T", "cells", ",", "suggesting", "a", "role", "for", "PAO-sensitive", "phosphatase", "in", "the", "activation", "of", "the", "NFkappaB", "via", "this", "pathway", "in", "human", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0, 7, 8, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 7, 8, 8, 0 ]
7710
[ "Furthermore", ",", "it", "demonstrates", "that", "aging", "does", "not", "influence", "the", "sensitivity", "of", "this", "phosphatase", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7711
[ "Treatment", "with", "DMP", "prior", "to", "treatment", "with", "PAO", "and", "TNF", "abolishes", "the", "inhibition", "induced", "by", "PAO", ",", "in", "T", "cells", "from", "both", "young", "and", "old", "donors", ",", "alike", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7712
[ "Finally", ",", "we", "demonstrate", "that", "a", "failure", "to", "degrade", "IkappaB-alpha", "in", "cytosols", "of", "TNF-treated", "T", "cells", "pretreated", "with", "PAO", "is", "due", "to", "its", "interference", "with", "the", "phosphorylation", "of", "IkappaB-alpha", "and", "not", "due", "to", "its", "inhibitory", "effect", "on", "proteasomal", "degradation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7713
[ "These", "data", "collectively", "suggest", "that", "PAO", "interferes", "with", "the", "phosphorylation", "and", "the", "regulated", "degradation", "of", "IkappaB-alpha", ",", "induced", "by", "TNF", ",", "without", "affecting", "the", "chymotryptic", "activity", "of", "the", "proteasome", ",", "independent", "of", "age", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7714
[ "Spi-C", ",", "a", "novel", "Ets", "protein", "that", "is", "temporally", "regulated", "during", "B", "lymphocyte", "development", "." ]
[ 9, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 7, 8, 0, 0 ]
7715
[ "A", "novel", "Ets", "protein", "was", "isolated", "by", "yeast", "one-hybrid", "screening", "of", "a", "cDNA", "library", "made", "from", "lipopolysaccharide-stimulated", "mouse", "splenic", "B", "cells", ",", "using", "the", "SP6", "kappa", "promoter", "kappaY", "element", "as", "a", "bait", "." ]
[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0 ]
7716
[ "The", "novel", "Ets", "protein", "was", "most", "closely", "related", "to", "PU.1", "and", "Spi-B", "within", "the", "DNA", "binding", "Ets", "domain", "and", "was", "therefore", "named", "Spi-C", "." ]
[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 0 ]
7717
[ "However", ",", "Spi-C", "may", "represent", "a", "novel", "subgroup", "within", "the", "Ets", "protein", "family", ",", "as", "it", "differed", "significantly", "from", "Spi-B", "and", "PU.1", "within", "helix", "1", "of", "the", "Ets", "domain", "." ]
[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 9, 10, 0 ]
7718
[ "Spi-C", "was", "encoded", "by", "a", "single-copy", "gene", "that", "was", "mapped", "to", "chromosome", "10", ",", "region", "C", "." ]
[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0 ]
7719
[ "Spi-C", "interacted", "with", "DNA", "similarly", "to", "PU.1", "as", "judged", "by", "methylation", "interference", ",", "band-shift", "and", "site", "selection", "analysis", ",", "and", "activated", "transcription", "of", "a", "kappaY", "element", "reporter", "gene", "upon", "co-transfection", "of", "HeLa", "cells", "." ]
[ 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]
7720
[ "Spi-C", "RNA", "was", "expressed", "in", "mature", "B", "lymphocytes", "and", "at", "lower", "levels", "in", "macrophages", "." ]
[ 3, 4, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 7, 0 ]
7721
[ "Furthermore", ",", "pre-B", "cell", "and", "plasma", "cell", "lines", "were", "Spi-C", "-negative", ",", "suggesting", "that", "Spi-C", "might", "be", "a", "regulatory", "molecule", "during", "a", "specific", "phase", "of", "B", "lymphoid", "development", "." ]
[ 0, 0, 7, 8, 0, 5, 6, 6, 0, 9, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7722
[ "Glucocorticoid-induced", "cell", "death", "requires", "autoinduction", "of", "glucocorticoid", "receptor", "expression", "in", "human", "leukemic", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 7, 8, 8, 8, 0 ]
7723
[ "In", "contrast", "to", "the", "negative", "autoregulation", "of", "glucocorticoid", "receptor", "(", "GR", ")", "expression", "seen", "in", "most", "cells", "and", "tissues", ",", "GR", "expression", "is", "positively", "autoregulated", "in", "human", "leukemic", "T", "cells", "and", "in", "other", "cells", "sensitive", "to", "glucocorticoid-induced", "cell", "death", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7724
[ "To", "determine", "whether", "positive", "autoregulation", "is", "a", "necessary", "component", "of", "glucocorticoid-induced", "cell", "death", ",", "a", "wild-type", "GR", "gene", "under", "the", "control", "of", "a", "tetracycline-regulated", "promoter", "was", "stably", "transfected", "into", "glucocorticoid-resistant", "cells", "lacking", "endogenous", "functional", "receptor", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7725
[ "Transfectants", "grown", "in", "the", "presence", "of", "tetracycline", "contained", "about", "15", ",", "000", "receptors/cell", ",", "a", "value", "approximately", "equal", "to", "basal", "level", "GR", "expression", "in", "glucocorticoid-sensitive", "6TG1.1", "cells", "before", "steroid", "treatment", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 5, 6, 0, 0, 0, 0 ]
7726
[ "Under", "these", "conditions", ",", "dexamethasone", "had", "a", "minimal", "effect", "on", "cell", "growth", ",", "elicited", "little", "internucleosomal", "DNA", "fragmentation", ",", "and", "induced", "no", "cell", "cycle", "perturbation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7727
[ "In", "the", "absence", "of", "tetracycline", ",", "GR", "mRNA", "and", "protein", "expression", "increased", "2-3-fold", ",", "and", "cells", "expressed", "48", ",", "000", "receptors", ",", "a", "level", "nearly", "equivalent", "to", "that", "present", "in", "6TG1.1", "cells", "after", "18", "h", "of", "autoinduction", "." ]
[ 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0 ]
7728
[ "Under", "these", "conditions", ",", "dexamethasone", "markedly", "inhibited", "cell", "growth", ",", "caused", "G1", "arrest", ",", "and", "induced", "significant", "internucleosomal", "DNA", "fragmentation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7729
[ "These", "studies", "therefore", "suggest", "that", "basal", "level", "GR", "expression", "is", "inadequate", "to", "mediate", "glucocorticoid-induced", "apoptosis", "in", "glucocorticoid-sensitive", "T", "cells", "and", "that", "positive", "autoregulation", "is", "a", "necessary", "component", "of", "this", "process", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7730
[ "Constitutive", "activation", "of", "NF-kappaB", "in", "primary", "adult", "T-cell", "leukemia", "cells", "." ]
[ 0, 0, 0, 9, 0, 0, 7, 8, 8, 8, 0 ]
7731
[ "Human", "T-cell", "leukemia", "virus", "type", "I", "(", "HTLV-I", ")", "is", "an", "etiologic", "agent", "of", "adult", "T-cell", "leukemia", "(", "ATL", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7732
[ "The", "viral", "protein", "Tax", "induces", "the", "activation", "and", "nuclear", "translocalization", "of", "transcription", "factor", "NF-kappaB", ",", "which", "is", "proposed", "to", "play", "a", "crucial", "role", "in", "the", "transformation", "of", "T", "cells", "by", "HTLV-I", "." ]
[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0 ]
7733
[ "However", ",", "the", "HTLV-I", "genes", "including", "Tax", "are", "not", "expressed", "significantly", "in", "primary", "leukemic", "cells", "from", "ATL", "patients", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0 ]
7734
[ "In", "this", "study", ",", "we", "examined", "the", "basis", "for", "NF-kappaB", "activation", "in", "freshly", "isolated", "leukemic", "cells", "from", "ATL", "patients", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0 ]
7735
[ "We", "found", "that", "leukemic", "cells", "from", "ATL", "patients", ",", "like", "HTLV-I-infected", "T-cell", "lines", ",", "display", "constitutive", "NF-kappaB", "DNA", "binding", "activity", "and", "increased", "degradation", "of", "IkappaBalpha", "(", "an", "inhibitor", "of", "NF-kappaB", ")", "." ]
[ 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 0 ]
7736
[ "Whereas", "the", "NF-kappaB", "binding", "activity", "in", "Tax-expressing", "T-cell", "lines", "consisted", "mostly", "of", "p50/c-Rel", ",", "fresh", "ATL", "samples", "contained", "p50/p50", "and", "p50/p65", "heterodimers", "." ]
[ 0, 0, 9, 0, 0, 0, 0, 5, 6, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 9, 10, 0 ]
7737
[ "One", "T-cell", "line", "derived", "from", "ATL", "leukemic", "cells", ",", "TL-Om1", ",", "displayed", "constitutive", "NF-kappaB", "activity", ",", "as", "well", "as", "enhanced", "degradation", "of", "IkappaBalpha", ",", "despite", "the", "lack", "of", "detectable", "Tax", "expression", "." ]
[ 0, 5, 6, 0, 0, 7, 8, 8, 0, 5, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]
7738
[ "Interestingly", ",", "the", "NF-kappaB", "in", "TL-Om1", "consists", "of", "p50/p50", "and", "p50/p65", "like", "that", "in", "fresh", "primary", "leukemic", "cells", "." ]
[ 0, 0, 0, 9, 0, 5, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 7, 8, 0 ]
7739
[ "Our", "results", "suggest", "that", "activation", "of", "NF-kappaB", "occurs", "through", "a", "Tax", "-independent", "mechanism", "in", "leukemic", "cells", "of", "ATL", "patients", ",", "possibly", "due", "to", "differential", "NF-kappaB", "subunit", "activation", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0 ]
7740
[ "Interferon-alpha", "induction", "of", "STATs1", ",", "-3", "DNA", "binding", "and", "growth", "arrest", "is", "independent", "of", "Lck", "and", "active", "mitogen-activated", "kinase", "in", "T", "cells", "." ]
[ 9, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 10, 0, 7, 8, 0 ]
7741
[ "Type", "I", "interferons", "(", "IFNs", ")", "are", "a", "family", "of", "cytokines", "that", "have", "antiviral", "and", "antiproliferative", "effects", "." ]
[ 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7742
[ "Data", "regarding", "the", "processes", "by", "which", "these", "cytokines", "transduce", "signals", "from", "the", "cell", "membrane", "to", "the", "nucleus", "are", "becoming", "increasingly", "complex", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7743
[ "The", "most", "characterized", "pathway", "is", "via", "JAK", "-STAT", "signaling", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]
7744
[ "Previous", "studies", "established", "a", "potential", "role", "for", "the", "Src-family", "kinase", "Lck", "in", "JAK", "-STAT", "signaling", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 10, 0, 0 ]
7745
[ "Therefore", ",", "this", "study", "was", "designed", "to", "analyze", "the", "role", "of", "Lck", "in", "IFN-alpha", "signaling", "by", "using", "the", "Jurkat", ",", "JCam", "(", "an", "Lck-defective", "cell", "line", "derived", "from", "Jurkat", ")", ",", "and", "JCam/Lck", "(", "JCam", "cells", "with", "Lck", "restored", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 5, 0, 5, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 5, 0, 5, 6, 0, 9, 0, 0, 0 ]
7746
[ "The", "results", "show", "that", "IFN-alpha", "can", "induce", "MAPK", "activity", ",", "but", "only", "in", "cells", "containing", "Lck", "." ]
[ 0, 0, 0, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
7747
[ "Furthermore", ",", "STATs1", "and", "-3", "are", "effectively", "phosphorylated", "and", "activated", "to", "bind", "DNA", "in", "the", "absence", "of", "Lck", "expression", "in", "IFN-alpha-treated", "cells", "." ]
[ 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0 ]
7748
[ "Finally", ",", "the", "results", "demonstrate", "that", "IFN-alpha", "exerts", "an", "antiproliferative", "effect", "in", "all", "three", "cell", "lines", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7749
[ "These", "data", "indicate", "that", "Lck", "and", "active", "MAPK", "do", "not", "affect", "IFN-alpha", "-induced", "growth", "arrest", "or", "induction", "of", "STAT1s1", "and", "-3", "DNA", "binding", "ability", "." ]
[ 0, 0, 0, 0, 9, 0, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0 ]
7750
[ "Copyright", "1999", "Academic", "Press", "." ]
[ 0, 0, 0, 0, 0 ]
7751
[ "Selective", "activation", "and", "functional", "significance", "of", "p38alpha", "mitogen-activated", "protein", "kinase", "in", "lipopolysaccharide-stimulated", "neutrophils", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 7, 0 ]
7752
[ "Activation", "of", "leukocytes", "by", "proinflammatory", "stimuli", "selectively", "initiates", "intracellular", "signal", "transduction", "via", "sequential", "phosphorylation", "of", "kinases", "." ]
[ 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7753
[ "Lipopolysaccharide", "(", "LPS", ")", "stimulation", "of", "human", "neutrophils", "is", "known", "to", "result", "in", "activation", "of", "p38", "mitogen-activated", "protein", "kinase", "(", "MAPk", ")", ";", "however", ",", "the", "upstream", "activator", "(", "s", ")", "of", "p38", "MAPk", "is", "unknown", ",", "and", "consequences", "of", "p38", "MAPk", "activation", "remain", "largely", "undefined", "." ]
[ 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0 ]
7754
[ "We", "investigated", "the", "MAPk", "kinase", "(", "MKK", ")", "that", "activates", "p38", "MAPk", "in", "response", "to", "LPS", ",", "the", "p38", "MAPk", "isoforms", "that", "are", "activated", "as", "part", "of", "this", "pathway", ",", "and", "the", "functional", "responses", "affected", "by", "p38", "MAPk", "activation", "." ]
[ 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]
7755
[ "Although", "MKK3", ",", "MKK4", ",", "and", "MKK6", "all", "activated", "p38", "MAPk", "in", "experimental", "models", ",", "only", "MKK3", "was", "found", "to", "activate", "recombinant", "p38", "MAPk", "in", "LPS-treated", "neutrophils", "." ]
[ 0, 9, 0, 9, 0, 0, 9, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 10, 0, 0, 7, 0 ]
7756
[ "Of", "p38", "MAPk", "isoforms", "studied", ",", "only", "p38alpha", "and", "p38delta", "were", "detected", "in", "neutrophils", "." ]
[ 0, 9, 10, 10, 0, 0, 0, 9, 0, 9, 0, 0, 0, 7, 0 ]
7757
[ "LPS", "stimulation", "selectively", "activated", "p38alpha", "." ]
[ 0, 0, 0, 0, 9, 0 ]
7758
[ "Specific", "inhibitors", "of", "p38alpha", "MAPk", "blocked", "LPS-induced", "adhesion", ",", "nuclear", "factor-kappa", "B", "(", "NF-kappaB", ")", "activation", ",", "and", "synthesis", "of", "tumor", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", "." ]
[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0 ]
7759
[ "Inhibition", "of", "p38alpha", "MAPk", "resulted", "in", "a", "transient", "decrease", "in", "TNF-alpha", "mRNA", "accumulation", "but", "persistent", "loss", "of", "TNF-alpha", "synthesis", "." ]
[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 9, 0, 0 ]
7760
[ "These", "findings", "support", "a", "pathway", "by", "which", "LPS", "stimulation", "of", "neutrophils", "results", "in", "activation", "of", "MKK3", ",", "which", "in", "turn", "activates", "p38alpha", "MAPk", ",", "ultimately", "regulating", "adhesion", ",", "NF-kappaB", "activation", ",", "enhanced", "gene", "expression", "of", "TNF-alpha", ",", "and", "regulation", "of", "TNF-alpha", "synthesis", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 0 ]
7761
[ "The", "evolutionarily", "conserved", "sequence", "upstream", "of", "the", "human", "Ig", "heavy", "chain", "S", "gamma", "3", "region", "is", "an", "inducible", "promoter", ":", "synergistic", "activation", "by", "CD40", "ligand", "and", "IL-4", "via", "cooperative", "NF-kappa", "B", "and", "STAT-6", "binding", "sites", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 1, 2, 0, 1, 2, 2, 0 ]
7762
[ "Germline", "C", "gamma", "gene", "transcription", "is", "a", "crucial", "event", "in", "the", "process", "that", "leads", "to", "switch", "DNA", "recombination", "to", "IgG", ",", "but", "its", "regulation", "in", "the", "human", "is", "poorly", "understood", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7763
[ "We", "took", "advantage", "of", "our", "monoclonal", "model", "of", "germinal", "center", "B", "cell", "differentiation", ",", "IgM+", "IgD+", "CL-01", "cells", ",", "to", "define", "the", "role", "of", "the", "I", "gamma", "3", "evolutionarily", "conserved", "sequence", "(", "ECS", ")", "in", "the", "germline", "transcriptional", "activation", "of", "the", "human", "C", "gamma", "3", "gene", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7764
[ "The", "I", "gamma", "3", "ECS", "lies", "upstream", "of", "the", "major", "I", "gamma", "3", "transcription", "initiation", "site", "and", "displays", "more", "than", "90", "%", "identity", "with", "the", "corresponding", "human", "I", "gamma", "1", ",", "I", "gamma", "2", ",", "and", "I", "gamma", "4", "regions", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7765
[ "Reporter", "luciferase", "gene", "vectors", "containing", "the", "human", "gamma", "3", "ECS", "were", "used", "to", "transfect", "CL-01", "cells", ",", "which", "have", "been", "shown", "to", "undergo", "Smu", "--", ">", "S", "gamma", "3", "DNA", "recombination", ",", "upon", "engagement", "of", "CD40", "by", "CD40", "ligand", "(", "CD40L", ")", "and", "exposure", "to", "IL-4", "." ]
[ 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 0, 9, 0, 0, 0, 0, 9, 0 ]
7766
[ "In", "these", "transfected", "CL-01", "cells", ",", "CD40", ":", "CD40L", "engagement", "and", "exposure", "to", "IL-4", "synergistically", "induced", "gamma", "3", "ECS-dependent", "luciferase", "reporter", "gene", "activation", "." ]
[ 0, 0, 0, 5, 6, 0, 9, 0, 9, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0 ]
7767
[ "Targeted", "mutational", "analysis", "demonstrated", "that", "a", "tandem", "NF-kappa", "B/Rel", "binding", "motif", "is", "critical", "for", "the", "gamma", "3", "ECS", "responsiveness", "to", "both", "CD40L", "and", "IL-4", ",", "while", "a", "STAT-6-binding", "site", "is", "additionally", "required", "for", "IL-4", "inducibility", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 1, 2, 0, 0, 0, 0, 9, 0, 0 ]
7768
[ "Electrophoretic", "mobility", "shift", "assays", "showed", "that", "p50/p65/c-Rel", "and", "STAT-6", "are", "effectively", "induced", "by", "CD40L", "and", "IL-4", ",", "respectively", ",", "and", "bind", "to", "specific", "DNA", "motifs", "within", "the", "ECS", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7769
[ "These", "partially", "overlapping", "CD40L", "and", "IL-4", "responsive", "elements", "are", "functionally", "cooperative", "as", "the", "disruption", "of", "one", "of", "them", "prevents", "synergistic", "promoter", "activation", "." ]
[ 0, 0, 0, 1, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7770
[ "Thus", ",", "the", "gamma", "3", "ECS", "is", "an", "inducible", "promoter", "containing", "cis", "elements", "that", "critically", "mediate", "CD40L", "and", "IL-4", "-triggered", "transcriptional", "activation", "of", "the", "human", "C", "gamma", "3", "gene", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7771
[ "CIITA", "-induced", "occupation", "of", "MHC", "class", "II", "promoters", "is", "independent", "of", "the", "cooperative", "stabilization", "of", "the", "promoter-bound", "multi-protein", "complexes", "." ]
[ 9, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7772
[ "Precise", "regulation", "of", "MHC", "class", "II", "expression", "plays", "a", "crucial", "role", "in", "the", "control", "of", "the", "immune", "response", "." ]
[ 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7773
[ "The", "transactivator", "CIITA", "behaves", "as", "a", "master", "controller", "of", "constitutive", "and", "inducible", "MHC", "class", "II", "gene", "activation", ",", "but", "its", "exact", "mechanism", "of", "action", "is", "not", "known", "." ]
[ 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7774
[ "Activation", "of", "MHC", "class", "II", "promoters", "requires", "binding", "of", "at", "least", "three", "distinct", "multi-protein", "complexes", "(", "RFX", ",", "X2BP", "and", "NF-Y", ")", "." ]
[ 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 9, 0, 0 ]
7775
[ "It", "is", "known", "that", "the", "stability", "of", "this", "binding", "results", "from", "cooperative", "interactions", "between", "these", "proteins", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7776
[ "We", "show", "here", "that", "expression", "of", "CIITA", "in", "MHC", "class", "II", "-cells", "triggers", "occupation", "of", "the", "promoters", "by", "these", "complexes", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7777
[ "This", "observation", "raised", "the", "possibility", "that", "the", "effect", "of", "CIITA", "on", "promoter", "occupation", "is", "mediated", "by", "an", "effect", "on", "the", "cooperative", "stabilization", "of", "the", "DNA-bound", "multi-protein", "complexes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7778
[ "We", "show", ",", "however", ",", "that", "the", "presence", "of", "CIITA", "does", "not", "affect", "the", "stability", "of", "the", "higher-order", "protein", "complex", "formed", "on", "DNA", "by", "RFX", ",", "X2BP", "and", "NF-Y", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 9, 0 ]
7779
[ "This", "suggests", "other", "mechanisms", "for", "CIITA", "-induced", "promoter", "occupancy", ",", "such", "as", "an", "effect", "on", "chromatin", "structure", "leading", "to", "increased", "accessibility", "of", "MHC", "class", "II", "promoters", "." ]
[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]
7780
[ "This", "ability", "of", "CIITA", "to", "facilitate", "promoter", "occupation", "is", "undissociable", "from", "its", "transactivation", "potential", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7781
[ "Finally", ",", "we", "conclude", "that", "this", "effect", "of", "CIITA", "is", "cell-type", "specific", ",", "since", "expression", "of", "CIITA", "is", "not", "required", "for", "normal", "occupation", "of", "MHC", "class", "II", "promoters", "in", "B", "lymphocytes", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 7, 8, 0 ]
7782
[ "GrpL", ",", "a", "Grb2", "-related", "adaptor", "protein", ",", "interacts", "with", "SLP-76", "to", "regulate", "nuclear", "factor", "of", "activated", "T", "cell", "activation", "." ]
[ 9, 0, 0, 9, 10, 10, 10, 0, 0, 0, 9, 0, 0, 9, 10, 10, 10, 10, 10, 0, 0 ]
7783
[ "Propagation", "of", "signals", "from", "the", "T", "cell", "antigen", "receptor", "(", "TCR", ")", "involves", "a", "number", "of", "adaptor", "molecules", "." ]
[ 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]
7784
[ "SH2", "domain-containing", "protein", "76", "(", "SLP-76", ")", "interacts", "with", "the", "guanine", "nucleotide", "exchange", "factor", "Vav", "to", "activate", "the", "nuclear", "factor", "of", "activated", "cells", "(", "NF-AT", ")", ",", "and", "its", "expression", "is", "required", "for", "normal", "T", "cell", "development", "." ]
[ 9, 10, 10, 10, 0, 9, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0 ]
7785
[ "We", "report", "the", "cloning", "and", "characterization", "of", "a", "novel", "Grb2-like", "adaptor", "molecule", "designated", "as", "Grb2-related", "protein", "of", "the", "lymphoid", "system", "(", "GrpL", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0, 0 ]
7786
[ "Expression", "of", "GrpL", "is", "restricted", "to", "hematopoietic", "tissues", ",", "and", "it", "is", "distinguished", "from", "Grb2", "by", "having", "a", "proline-rich", "region", "." ]
[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0 ]
7787
[ "GrpL", "can", "be", "coimmunoprecipitated", "with", "SLP-76", "but", "not", "with", "Sos1", "or", "Sos2", "from", "Jurkat", "cell", "lysates", "." ]
[ 9, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 9, 0, 5, 6, 0, 0 ]
7788
[ "In", "contrast", ",", "Grb2", "can", "be", "coimmunoprecipitated", "with", "Sos1", "and", "Sos2", "but", "not", "with", "SLP-76", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 0 ]
7789
[ "Moreover", ",", "tyrosine-phosphorylated", "LAT/pp36/38", "in", "detergent", "lysates", "prepared", "from", "anti-CD3", "stimulated", "T", "cells", "associated", "with", "Grb2", "but", "not", "GrpL", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 7, 8, 0, 0, 9, 0, 0, 9, 0 ]
7790
[ "These", "data", "reveal", "the", "presence", "of", "distinct", "complexes", "involving", "GrpL", "and", "Grb2", "in", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 7, 8, 0 ]
7791
[ "A", "functional", "role", "of", "the", "GrpL-SLP-76", "complex", "is", "suggested", "by", "the", "ability", "of", "GrpL", "to", "act", "alone", "or", "in", "concert", "with", "SLP-76", "to", "augment", "NF-AT", "activation", "in", "Jurkat", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0, 0, 5, 6, 6, 0 ]
7792
[ "Differential", "induction", "of", "interferon", "(", "IFN", ")", "-inducible", "protein", "10", "following", "differentiation", "of", "a", "monocyte", ",", "macrophage", "cell", "lineage", "is", "related", "to", "the", "changes", "of", "nuclear", "proteins", "bound", "to", "IFN", "stimulus", "response", "element", "and", "kappaB", "sites", "." ]
[ 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 7, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0 ]
7793
[ "We", "examined", "chemokine", "gene", "expression", "following", "the", "differentiation", "of", "a", "monocyte", ",", "macrophage", "cell", "lineage", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 7, 8, 8, 0 ]
7794
[ "The", "human", "monoblastic", "cell", "line", ",", "U937", "was", "differentiated", "to", "macrophages", "by", "the", "treatment", "with", "either", "phorbol", "12-myristate", "13-acetate", "(", "PMA", ")", ",", "retinoic", "acid", "(", "RA", ")", ",", "or", "vitamin", "D3", "(", "VitD3", ")", "." ]
[ 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7795
[ "The", "gene", "expression", "of", "interferon", "(", "IFN", ")", "-inducible", "protein", "10", "(", "IP-10", ")", "(", "a", "CXC", "chemokine", ")", "was", "markedly", "augmented", "by", "the", "IFNgamma", "treatment", "in", "PMA", "-or", "RA-differentiated", "U937", "cells", ",", "but", "only", "marginally", "in", "undifferentiated", "or", "VitD3-treated", "cells", "." ]
[ 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
7796
[ "In", "contrast", ",", "another", "inducible", "gene", "expression", "of", "monocyte", "chemotactic", "protein-1", "(", "a", "CC", "chemokine", ")", "and", "the", "activation", "of", "the", "transcriptional", "factor", "(", "FcRFgamma", ")", "bound", "to", "the", "gamma", "response", "region", "were", "similarly", "or", "less", "abundantly", "induced", "by", "IFNgamma", "treatment", "in", "PMA", "-or", "RA-differentiated", "U937", "cells", ",", "indicating", "that", "increased", "IP-10", "mRNA", "induction", "was", "not", "due", "to", "the", "augmented", "ability", "of", "the", "cells", "to", "respond", "to", "the", "presence", "of", "IFNgamma", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
7797
[ "Increased", "expression", "of", "IFNgamma", "-induced", "IP-10", "mRNA", "following", "the", "differentiation", "of", "U937", "cells", "was", "mediated", "largely", "by", "augmented", "transcriptional", "activity", "of", "the", "gene", "and", "was", "related", "to", "differentiation-dependent", "changes", "of", "the", "proteins", "bound", "to", "IFN", "stimulus", "response", "element", "(", "ISRE", ")", "and", "kB", "sites", ",", "suggesting", "that", "these", "nuclear", "proteins", "may", "determine", "the", "IP-10", "mRNA", "inducibility", "by", "IFNgamma", "." ]
[ 0, 0, 0, 9, 0, 3, 4, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 9, 0 ]
7798
[ "Role", "of", "cellular", "tumor", "necrosis", "factor", "receptor-associated", "factors", "in", "NF-kappaB", "activation", "and", "lymphocyte", "transformation", "by", "herpesvirus", "Saimiri", "STP", "." ]
[ 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 0, 0, 7, 0, 0, 9, 10, 10, 0 ]
7799
[ "The", "STP", "oncoproteins", "of", "the", "herpesvirus", "saimiri", "(", "HVS", ")", "subgroup", "A", "strain", "11", "and", "subgroup", "C", "strain", "488", "are", "now", "found", "to", "be", "stably", "associated", "with", "tumor", "necrosis", "factor", "receptor-associated", "factor", "(", "TRAF", ")", "1", ",", "2", ",", "or", "3", "." ]
[ 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 0, 9, 0, 0, 9, 0 ]
7800
[ "Mutational", "analyses", "identified", "residues", "of", "PXQXT/S", "in", "STP-A11", "as", "critical", "for", "TRAF", "association", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 0 ]