Datasets:
siddharthtumre
/
Revised-JNLPBA

Dataset card Data Studio Files Community
1
id
stringlengths
1
5
tokens
sequence
ner_tags
sequence
14301
[ "Occurrence", "of", "a", "silencer", "of", "the", "interleukin-2", "gene", "in", "naive", "but", "not", "in", "memory", "resting", "T", "helper", "lymphocytes", "." ]
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14302
[ "In", "the", "immune", "system", "the", "first", "activation", "of", "a", "naive", "T", "cell", "by", "antigen", "is", "a", "key", "step", "in", "the", "shaping", "of", "the", "peripheral", "T", "cell", "specificity", "repertoire", "and", "maintenance", "of", "self-tolerance", "." ]
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14303
[ "In", "the", "present", "study", ",", "analysis", "of", "the", "interleukin-2", "(", "IL-2", ")", "gene", "activation", "shows", "that", "naive", "human", "helper", "T", "cells", "(", "cord", "blood", "CD4+", "T", "cells", ",", "adult", "CD4+CD45RO-", "T", "cells", ")", "regulate", "IL-2", "transcription", "by", "a", "mechanism", "involving", "both", "a", "silencer", "and", "an", "activator", "acting", "on", "the", "purine-rich", "IL-2", "promoter", "elements", "(", "NF-AT", "binding", "sites", ")", "." ]
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14304
[ "By", "contrast", ",", "memory", "cells", ",", "either", "in", "vitro", "activated", "helper", "T", "cells", "reverting", "to", "a", "resting", "state", ",", "or", "CD4+", "T", "(", "memory", ")", "clones", ",", "or", "CD4+CD45RO+", "T", "cells", "isolated", "ex", "vivo", ",", "no", "longer", "have", "a", "silencer", "." ]
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14305
[ "Their", "IL-2", "transcription", "seems", "to", "be", "controlled", "solely", "by", "the", "transition", "from", "inactive", "to", "active", "functional", "state", "of", "a", "positive", "transcription", "factor", "binding", "to", "these", "promoter", "elements", "as", "well", "as", "its", "cytoplasmic", "or", "nuclear", "location", ":", "in", "resting", "memory", "T", "cells", "the", "activator", "is", "located", "in", "the", "cytoplasm", "and", "is", "inactive", ",", "whereas", "in", "stimulated", "cells", "it", "is", "functional", "in", "promoting", "transcription", "and", "now", "resides", "in", "the", "nucleus", "." ]
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14306
[ "Thus", ",", "the", "regulation", "of", "the", "gene", "coding", "for", "the", "main", "T", "cell", "growth", "factor", "changes", "irreversibly", "after", "the", "first", "encounter", "of", "T", "cells", "with", "antigen", "." ]
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14307
[ "It", "is", "most", "likely", "that", "the", "presence", "of", "a", "silencer", "contributes", "to", "the", "more", "stringent", "activation", "requirements", "of", "naive", "CD4+", "T", "cells", "." ]
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14308
[ "Expression", "of", "mRNA", "for", "the", "GATA-binding", "proteins", "in", "human", "eosinophils", "and", "basophils", ":", "potential", "role", "in", "gene", "transcription", "." ]
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14309
[ "The", "expression", "of", "the", "hematopoietic", "transcription", "factors", "GATA-1", ",", "GATA-2", ",", "and", "GATA-3", "was", "studied", "in", "eosinophils", "and", "basophils", "." ]
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14310
[ "Eosinophils", "express", "mRNA", "for", "GATA-1", ",", "GATA-2", ",", "and", "GATA-3", "." ]
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14311
[ "Basophils", "express", "GATA-2", "and", "GATA-3", "." ]
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14312
[ "Treatment", "of", "HL-60", "eosinophilic", "sublines", "with", "either", "interleukin-5", "or", "butyric", "acid", "increased", "the", "expression", "of", "GATA-1", "mRNA", "concomitant", "with", "the", "expression", "of", "eosinophil-specific", "genes", ",", "whereas", "levels", "of", "GATA-2", "mRNA", "remained", "relatively", "constant", "." ]
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14313
[ "The", "presence", "of", "mRNA", "for", "these", "proteins", "in", "eosinophils", "and", "basophils", "suggests", "that", "gene", "transcription", "in", "these", "lineages", "may", "be", "regulated", "by", "GATA-binding", "proteins", "." ]
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14314
[ "Oxidoreductive", "regulation", "of", "nuclear", "factor", "kappa", "B", "." ]
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14315
[ "Involvement", "of", "a", "cellular", "reducing", "catalyst", "thioredoxin", "." ]
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14316
[ "We", "have", "investigated", "an", "oxidoreductive", "regulatory", "pathway", "for", "the", "DNA", "binding", "activity", "of", "a", "pleiotropic", "cellular", "transcription", "factor", ",", "nuclear", "factor", "kappa", "B", "(", "NF", "kappa", "B", ")", ",", "has", "been", "investigated", "by", "using", "NF", "kappa", "B", "prepared", "from", "the", "nucleus", "and", "the", "cytosol", "of", "the", "primary", "human", "T", "lymphocytes", "." ]
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14317
[ "We", "show", "that", "a", "cellular", "reducing", "catalyst", "thioredoxin", "(", "Trx", ")", "plays", "a", "major", "role", "in", "activation", "of", "the", "DNA", "binding", "of", "NF", "kappa", "B", "in", "vitro", "and", "stimulation", "of", "transcription", "from", "the", "NF", "kappa", "B", "-dependent", "gene", "expression", "." ]
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14318
[ "We", "demonstrate", "evidence", "suggesting", "that", "redox", "regulation", "of", "NF", "kappa", "B", "by", "Trx", "might", "be", "exerted", "at", "a", "step", "after", "dissociation", "of", "the", "inhibitory", "molecule", "I", "kappa", "B", ",", "a", "cytosolic-anchoring", "protein", "for", "NF", "kappa", "B", "." ]
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14319
[ "To", "examine", "the", "effect", "of", "Trx", "in", "intact", "cells", ",", "we", "performed", "transient", "assay", "with", "a", "chloramphenicol", "acetyltransferase-expressing", "plasmid", "under", "the", "control", "of", "human", "immunodeficiency", "virus", "(", "HIV", ")", "long", "terminal", "repeat", "and", "an", "effector", "plasmid", "expressing", "human", "Trx", "." ]
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14320
[ "The", "promoter", "activity", "from", "HIV", "long", "terminal", "repeat", "was", "greatly", "augmented", "by", "co-transfecting", "the", "Trx-expressing", "plasmid", ",", "whose", "effect", "was", "dependent", "on", "the", "NF", "kappa", "B", "-binding", "sites", "." ]
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14321
[ "These", "findings", "have", "suggested", "that", "cysteine", "residue", "(", "s", ")", "of", "NF", "kappa", "B", "might", "be", "involved", "in", "the", "DNA-recognition", "by", "NF", "kappa", "B", "and", "that", "the", "redox", "control", "mechanism", "mediated", "by", "Trx", "might", "have", "a", "regulatory", "role", "in", "the", "NF", "kappa", "B", "-mediated", "gene", "expression", "." ]
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14322
[ "These", "results", "may", "also", "provide", "a", "clue", "to", "understanding", "of", "the", "molecular", "process", "of", "AIDS", "pathogenesis", "and", "its", "possible", "biochemical", "intervention", "." ]
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14323
[ "Synergism", "between", "the", "CD3", "antigen-", "and", "CD2", "antigen-derived", "signals", "." ]
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14324
[ "Exploration", "at", "the", "level", "of", "induction", "of", "DNA-binding", "proteins", "and", "characterization", "of", "the", "inhibitory", "activity", "of", "cyclosporine", "." ]
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14325
[ "We", "have", "demonstrated", "earlier", "that", "the", "crosslinkage", "of", "the", "CD3/TCR", "complex", "with", "the", "CD2", "antigen", "results", "in", "the", "proliferation", "of", "normal", "human", "T", "cells", "." ]
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14326
[ "The", "effect", "of", "this", "synergism", "was", "perceptible", "at", "the", "level", "of", "induction", "of", "the", "IL-2", "gene", ",", "a", "process", "critical", "for", "T", "cell", "growth", "." ]
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14327
[ "To", "further", "understand", "the", "molecular", "and", "nuclear", "basis", "for", "this", "synergism", ",", "we", "have", "explored", "the", "induction", "of", "DNA-binding", "proteins", "in", "highly", "purified", "normal", "human", "T", "cells", "signaled", "via", "the", "CD3", "and/or", "CD2", "proteins", "." ]
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14328
[ "The", "effect", "of", "transmembrane", "signaling", "of", "T", "cells", "with", "ionomycin", ",", "and/or", "sn-1", ",", "2", "dioctanoyl", "glycerol", ",", "was", "also", "determined", "." ]
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14329
[ "The", "emergence", "of", "nuclear", "binding", "proteins", "was", "investigated", "using", "interleukin-2", "sequence", "specific", "oligonucleotide", "probes", "in", "the", "electrophoretic", "mobility", "shift", "assay", "." ]
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14330
[ "Our", "studies", "demonstrate", "for", "the", "first", "time", "that", "CD3", "antigen-derived", "signals", "and", "CD2", "antigen", "-derived", "signals", "are", "synergistic", "in", "inducing", "the", "emergence", "of", "transcription", "factors", "that", "bind", "to", "the", "NF-AT1", ",", "AP-1", ",", "and", "NF-kB", "sites", "located", "in", "the", "promoter/enhancer", "region", "of", "the", "IL-2", "gene", "." ]
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14331
[ "Moreover", ",", "cyclosporine", ",", "at", "concentrations", "readily", "accomplished", "in", "clinical", "practice", ",", "was", "found", "to", "inhibit", "the", "emergence", "of", "these", "DNA-binding", "proteins", "in", "normal", "human", "T", "cells", "signaled", "via", "cell", "surface", "proteins", "implicated", "in", "antigen-dependent", "T", "cell", "activation", "and", "in", "T", "cells", "stimulated", "by", "mobilization", "of", "cellular", "calcium", "and", "activation", "of", "protein", "kinase", "C", "." ]
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14332
[ "Induced", "myeloid", "differentiation", "of", "K562", "cells", "with", "downregulation", "of", "erythroid", "and", "megakaryocytic", "transcription", "factors", ":", "a", "novel", "experimental", "model", "for", "hemopoietic", "lineage", "restriction", "." ]
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14333
[ "The", "human", "erythroleukemia", "cell", "line", "K562", "can", "be", "induced", "to", "differentiate", "along", "the", "erythroid", "and", "megakaryocytic", "lineages", "." ]
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14334
[ "Here", "we", "demonstrate", "that", "hexamethylene", "bisacetamide", "(", "HMBA", ")", "induced", "K562", "cells", "to", "differentiate", "along", "a", "third", "pathway", "." ]
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14335
[ "This", "was", "accompanied", "by", "downregulation", "of", "two", "transcription", "factors", "normally", "expressed", "in", "erythroid", ",", "mast", "and", "megakaryocyte", "lineages", "." ]
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14336
[ "Northern", "analysis", "demonstrated", "coordinate", "downregulation", "of", "alpha", "globin", "and", "gamma", "globin", "in", "addition", "to", "the", "two", "lineage-restricted", "transcription", "factors", ",", "SCL", "and", "GATA-1", "." ]
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14337
[ "Proliferation", "of", "the", "K562", "cells", "was", "also", "suppressed", "." ]
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14338
[ "Clonal", "assay", "showed", "that", "the", "suppression", "was", "irreversible", "and", "appeared", "analogous", "to", "the", "commitment", "of", "murine", "erythroleukemia", "(", "MEL", ")", "cells", "to", "terminal", "differentiation", "." ]
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14339
[ "In", "contrast", "to", "MEL", "cells", ",", "however", ",", "K562", "cells", "acquired", "a", "macrophage-like", "morphology", "and", "exhibited", "a", "complete", "failure", "to", "generate", "benzidine-positive", "cells", "." ]
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14340
[ "Electron", "microscopy", "revealed", "a", "marked", "increase", "in", "granules", "resembling", "those", "specific", "for", "eosinophils", "." ]
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14341
[ "Surface", "marker", "analysis", "showed", "that", "HMBA-induced", "cells", "expressed", "reduced", "levels", "of", "glycophorin", "A", ",", "CD5", ",", "CD7", "and", "CD11b", "." ]
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14342
[ "No", "upregulation", "of", "megakaryocyte", "or", "lymphoid", "markers", "occurred", "." ]
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14343
[ "Thus", "the", "response", "of", "K562", "cells", "to", "HMBA", "may", "provide", "a", "useful", "experimental", "system", "for", "studying", "the", "molecular", "mechanisms", "responsible", "for", "downmodulation", "of", "lineage-restricted", "transcription", "factors", "during", "hemopoietic", "lineage", "commitment", "." ]
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14344
[ "Activation", "of", "primary", "human", "T-lymphocytes", "through", "CD2", "plus", "CD28", "adhesion", "molecules", "induces", "long-term", "nuclear", "expression", "of", "NF-kappa", "B", "." ]
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14345
[ "Stimulation", "of", "highly", "purified", "human", "T-cells", "via", "CD2", "and", "CD28", "adhesion", "molecules", "induces", "and", "maintains", "proliferation", "for", "more", "than", "3", "weeks", "." ]
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14346
[ "This", "potent", "interleukin", "2", "(", "IL-2", ")", "-dependent", "activation", "does", "not", "require", "monocytes", "or", "accessory", "cells", "." ]
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14347
[ "Long-lasting", "IL-2", "receptivity", "is", "associated", "with", "high-level", "expression", "of", "the", "inducible", "IL-2", "receptor", "alpha", "chain", "(", "IL-2R", "alpha", ")", "gene", "that", "is", "regulated", "at", "both", "transcriptional", "and", "posttranscriptional", "levels", "." ]
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14348
[ "Increase", "of", "IL-2R", "alpha", "gene", "transcription", "involves", "the", "enhanced", "binding", "of", "the", "transcription", "factor", "NF-kappa", "B", "to", "its", "consensus", "sequence", "in", "the", "5'-regulatory", "region", "of", "the", "IL-2R", "alpha", "gene", "." ]
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14349
[ "To", "dissect", "the", "molecular", "basis", "for", "the", "unusually", "persistent", "transcription", "of", "the", "IL-2R", "alpha", "gene", ",", "we", "analyzed", "nuclear", "NF-kappa", "B", "binding", "to", "a", "radiolabeled", "IL-2R", "alpha", "kappa", "B-specific", "oligonucleotide", "probe", "during", "the", "time", "course", "of", "CD2", "+", "CD28", "activation", "." ]
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14350
[ "Resting", "T-cell", "nuclear", "extracts", "contained", "KBF1/p50", "homodimer", "." ]
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14351
[ "After", "stimulation", ",", "two", "new", "kappa", "B-specific", "complexes", "were", "identified", "as", "NF-kappa", "B", "p50-p65", "heterodimer", "and", "putative", "c-Rel", "homodimer", "or", "c-Rel-p65", "heterodimer", "." ]
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14352
[ "Both", "inducible", "complexes", "persisted", "for", "at", "least", "3", "weeks", "." ]
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14353
[ "Their", "relative", "levels", "were", "very", "similar", "for", "the", "duration", "of", "proliferation", "." ]
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14354
[ "In", "parallel", ",", "CD2", "+", "CD28", "activation", "triggered", "a", "significant", "intracellular", "thiol", "decrease", ",", "suggesting", "that", "oxygen", "radicals", "are", "involved", "in", "the", "signaling", "pathway", "of", "adhesion", "molecules", "." ]
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