id
stringlengths
1
5
tokens
sequence
ner_tags
sequence
14101
[ "In", "particular", ",", "naturally", "occurring", "sequence", "variation", "impacted", "transcription", "factor", "binding", "to", "an", "activating", "transcription", "factor/cAMP", "response", "element", "binding", "(", "ATF/CREB", ")", "binding", "site", "(", "located", "between", "the", "LEF-1", "and", "distal", "NF-kappaB", "transcription", "factor", "binding", "sites", ")", "that", "we", "identified", "in", "previous", "studies", "of", "the", "HIV-1", "LTR", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 1, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]
14102
[ "These", "findings", "suggest", "that", "LTR", "sequence", "changes", "can", "significantly", "affect", "basal", "LTR", "function", "and", "transcription", "factor", "recruitment", ",", "which", "may", ",", "in", "turn", ",", "alter", "the", "course", "of", "viral", "replication", "in", "cells", "of", "CNS", "and", "immune", "system", "origin", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14103
[ "HMG", "box", "containing", "transcription", "factors", "in", "lymphocyte", "differentiation", "." ]
[ 9, 10, 10, 10, 10, 0, 7, 0, 0 ]
14104
[ "The", "identification", "of", "the", "mammalian", "sex-determining", "gene", "Sry", "has", "led", "to", "the", "discovery", "of", "a", "large", "family", "of", "related", "(", "'", "HMG", "box", "'", ")", "transcription", "factors", "that", "control", "developmental", "events", "in", "yeast", ",", "C.", "elegans", ",", "Drosophila", "and", "vertebrates", "." ]
[ 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14105
[ "In", "lymphocyte", "differentiation", ",", "several", "HMG", "box", "proteins", "play", "a", "decisive", "role", "." ]
[ 0, 7, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0 ]
14106
[ "Sox-4", "is", "important", "for", "very", "early", "B-cell", "differentiation", ",", "while", "TCF-1/LEF-1", "play", "a", "crucial", "role", "in", "early", "thymocyte", "development", "." ]
[ 9, 0, 0, 0, 0, 0, 7, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 7, 0, 0 ]
14107
[ "TCF/LEF", "proteins", "have", "recently", "been", "found", "to", "constitute", "a", "downstream", "component", "of", "the", "Wingless/Wnt", "signal", "transduction", "pathway", "." ]
[ 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]
14108
[ "In", "flies", ",", "this", "pathway", "controls", "segment", "polarity", ";", "in", "Xenopus", "it", "controls", "the", "definition", "of", "the", "body", "axis", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14109
[ "Deregulation", "of", "the", "pathway", "occurs", "in", "several", "human", "tumors", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14110
[ "These", "insights", "in", "the", "molecular", "events", "that", "are", "involved", "in", "TCF/LEF", "function", "in", "these", "organisms", "may", "eventually", "lead", "to", "the", "understanding", "of", "the", "function", "of", "these", "HMG", "box", "proteins", "in", "lymphoid", "development" ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0 ]
14111
[ "Single", "strand", "conformation", "polymorphism", "analysis", "of", "androgen", "receptor", "gene", "mutations", "in", "patients", "with", "androgen", "insensitivity", "syndromes", ":", "application", "for", "diagnosis", ",", "genetic", "counseling", ",", "and", "therapy", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14112
[ "Recent", "studies", "indicate", "that", "mutations", "in", "the", "androgen", "receptor", "gene", "are", "associated", "with", "androgen", "insensitivity", "syndromes", ",", "a", "heterogeneous", "group", "of", "related", "disorders", "involving", "defective", "sexual", "differentiation", "in", "karyotypic", "males", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14113
[ "In", "this", "report", ",", "we", "address", "the", "possibility", "of", "rapid", "mutational", "analysis", "of", "the", "androgen", "receptor", "gene", "for", "initial", "diagnosis", ",", "genetic", "counseling", ",", "and", "molecular", "subclassification", "of", "affected", "patients", "and", "their", "families", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14114
[ "DNA", "from", "peripheral", "blood", "leukocytes", "of", "six", "patients", "from", "five", "families", "with", "various", "degrees", "of", "androgen", "insensitivity", "was", "studied", "." ]
[ 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14115
[ "Exons", "2", "to", "8", "of", "the", "androgen", "receptor", "gene", "were", "analyzed", "using", "a", "combination", "of", "single", "strand", "conformation", "polymorphism", "analysis", "and", "direct", "DNA", "sequencing", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14116
[ "Female", "family", "members", "were", "also", "studied", "to", "identify", "heterozygote", "carriers", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14117
[ "Point", "mutations", "in", "the", "AR", "gene", "were", "identified", "in", "all", "six", "patients", ",", "and", "all", "mutations", "caused", "amino", "acid", "substitutions", "." ]
[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14118
[ "One", "patient", "with", "incomplete", "androgen", "insensitivity", "was", "a", "mosaic", "for", "the", "mutation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14119
[ "Four", "of", "the", "five", "mothers", ",", "as", "well", "as", "a", "young", "sister", "of", "one", "patient", ",", "were", "carriers", "of", "the", "mutation", "present", "in", "the", "affected", "child", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14120
[ "Our", "data", "show", "that", "new", "mutations", "may", "occur", "in", "the", "androgen", "receptor", "gene", "leading", "to", "sporadic", "androgen", "insensitivity", "syndrome", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0 ]
14121
[ "Molecular", "genetic", "characterization", "of", "the", "variant", "allele", "can", "serve", "as", "a", "primary", "tool", "for", "diagnosis", "and", "subsequent", "therapy", ",", "and", "can", "provide", "a", "basis", "for", "distinguishing", "heterozygous", "carriers", "in", "familial", "androgen", "resistance", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14122
[ "The", "identification", "of", "carriers", "is", "of", "substantial", "clinical", "importance", "for", "genetic", "counseling", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14123
[ "Proliferation", "index", "as", "a", "prognostic", "marker", "in", "breast", "cancer", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14124
[ "BACKGROUND", "." ]
[ 0, 0 ]
14125
[ "The", "proliferative", "activity", "of", "tumors", "has", "been", "extensively", "investigated", "with", "different", "approaches", ",", "among", "which", "the", "use", "of", "the", "monoclonal", "antibody", "Ki-67", "represents", "an", "easy", "and", "reliable", "means", "of", "assessing", "cell", "proliferation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14126
[ "In", "this", "study", ",", "the", "proliferative", "activity", "of", "129", "primary", "breast", "cancers", "was", "investigated", ",", "and", "the", "results", "were", "related", "to", "prognosis", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14127
[ "METHODS", "." ]
[ 0, 0 ]
14128
[ "Tumor", "samples", ",", "obtained", "from", "129", "patients", "who", "underwent", "surgery", "between", "January", "1987", "and", "December", "1988", ",", "were", "processed", "for", "staining", "by", "an", "immunohistochemical", "procedure", "(", "avidin-biotin", "complex", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14129
[ "The", "median", "time", "of", "observation", "was", "42", "months", "(", "range", ",", "31-55", "months", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14130
[ "Life-table", "analysis", "(", "Mantel-Cox", ")", "was", "used", "to", "assess", "the", "probability", "of", "disease-free", "survival", "(", "DFS", ")", "and", "overall", "survival", "(", "OS", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14131
[ "RESULTS", "." ]
[ 0, 0 ]
14132
[ "Tumors", "with", "high", "Ki-67", "proliferation", "indices", "(", ">", "20", "%", ")", "were", "associated", "with", "a", "higher", "4-year", "probability", "of", "relapse", "of", "disease", "(", "55.3", "%", "versus", "79.1", "%", ";", "P", "=", "0.003", ")", "and", "death", "(", "71", "%", "versus", "95.6", "%", ";", "P", "=", "0.00005", ")", "when", "compared", "with", "tumors", "with", "low", "Ki-67", "values", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]
14133
[ "In", "addition", ",", "this", "proliferative", "parameter", "maintained", "its", "prognostic", "significance", "when", "the", "patients", "were", "stratified", "according", "to", "lymph", "node", "involvement", ",", "menopausal", "status", ",", "and", "nuclear", "estrogen", "receptor", "content", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]
14134
[ "CONCLUSIONS", "." ]
[ 0, 0 ]
14135
[ "Tumor", "proliferative", "activity", "as", "evaluated", "by", "the", "monoclonal", "antibody", "Ki-67", "seems", "to", "be", "an", "effective", "indicator", "of", "prognosis", "in", "breast", "cancer", "for", "DFS", "and", "OS", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14136
[ "Regulation", "of", "the", "Ets-related", "transcription", "factor", "Elf-1", "by", "binding", "to", "the", "retinoblastoma", "protein", "." ]
[ 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0 ]
14137
[ "The", "retinoblastoma", "gene", "product", "(", "Rb", ")", "is", "a", "nuclear", "phosphoprotein", "that", "regulates", "cell", "cycle", "progression", "." ]
[ 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14138
[ "Elf-1", "is", "a", "lymphoid-specific", "Ets", "transcription", "factor", "that", "regulates", "inducible", "gene", "expression", "during", "T", "cell", "activation", "." ]
[ 9, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0 ]
14139
[ "In", "this", "report", ",", "it", "is", "demonstrated", "that", "Elf-1", "contains", "a", "sequence", "motif", "that", "is", "highly", "related", "to", "the", "Rb", "binding", "sites", "of", "several", "viral", "oncoproteins", "and", "binds", "to", "the", "pocket", "region", "of", "Rb", "both", "in", "vitro", "and", "in", "vivo", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]
14140
[ "Elf-1", "binds", "exclusively", "to", "the", "underphosphorylated", "form", "of", "Rb", "and", "fails", "to", "bind", "to", "Rb", "mutants", "derived", "from", "patients", "with", "retinoblastoma", "." ]
[ 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]
14141
[ "Co-immunoprecipitation", "experiments", "demonstrated", "an", "association", "between", "Elf-1", "and", "Rb", "in", "resting", "normal", "human", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 7, 8, 8, 0 ]
14142
[ "After", "T", "cell", "activation", ",", "the", "phosphorylation", "of", "Rb", "results", "in", "the", "release", "of", "Elf-1", ",", "which", "is", "correlated", "temporally", "with", "the", "activation", "of", "Elf-1", "-mediated", "transcription", "." ]
[ 0, 7, 8, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0 ]
14143
[ "Overexpression", "of", "a", "phosphorylation-defective", "form", "of", "Rb", "inhibited", "Elf-1", "-dependent", "transcription", "during", "T", "cell", "activation", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 7, 8, 0, 0 ]
14144
[ "These", "results", "demonstrate", "that", "Rb", "interacts", "specifically", "with", "a", "lineage-restricted", "Ets", "transcription", "factor", "." ]
[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]
14145
[ "This", "regulated", "interaction", "may", "be", "important", "for", "the", "coordination", "of", "lineage-specific", "effector", "functions", "such", "as", "lymphokine", "production", "with", "cell", "cycle", "progression", "in", "activated", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
14146
[ "Human", "CD3-CD16+", "natural", "killer", "cells", "express", "the", "hGATA-3", "T", "cell", "transcription", "factor", "and", "an", "unrearranged", "2.3-kb", "TcR", "delta", "transcript", "." ]
[ 7, 8, 8, 8, 8, 0, 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 3, 4, 4, 0 ]
14147
[ "In", "this", "study", "we", "analyzed", "the", "T", "cell", "receptor", "(", "TcR", ")", "delta", "transcripts", "expressed", "by", "CD3-CD16+", "cells", "and", "we", "investigated", "whether", "these", "cells", "expressed", "the", "hGATA-3", "T", "cell", "transcription", "factor", "and", "the", "recombination-activating", "gene", "(", "RAG", ")", "-1", "." ]
[ 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 4, 4, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 1, 2, 2, 2, 2, 2, 0 ]
14148
[ "Multiple", "TcR", "delta", "transcripts", "deriving", "from", "an", "unrearranged", "TcR", "delta", "gene", "were", "detected", "in", "both", "polyclonal", "and", "clonal", "CD3-CD16+", "natural", "killer", "(", "NK", ")", "cell", "lines", "." ]
[ 0, 3, 4, 4, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 0 ]
14149
[ "Two", "unrearranged", "TcR", "delta", "transcripts", "had", "a", "size", "similar", "to", "that", "of", "the", "functional", "TcR", "delta", "mRNA", "(", "2.3", "and", "1.3", "kb", ")", "found", "in", "TcR", "gamma/delta+", "T", "lymphocytes", "." ]
[ 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
14150
[ "Sequence", "analysis", "of", "nine", "different", "2.3-kb", "cDNA", "clones", "obtained", "from", "NK-derived", "polyA+", "RNA", "confirmed", "that", "they", "corresponded", "to", "an", "unrearranged", "TcR", "delta", "gene", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0 ]
14151
[ "These", "cDNA", "were", "2343", "bp", "long", "and", "their", "transcription", "initiation", "site", "was", "located", "814", "bp", "upstream", "from", "the", "J", "delta", "1", "segment", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14152
[ "The", "sequence", "located", "upstream", "of", "the", "J", "delta", "1", "segment", "corresponded", "to", "the", "previously", "reported", "germ-line", "sequence", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14153
[ "The", "J", "delta", "1", "segment", "was", "correctly", "spliced", "to", "C", "delta", ";", "in", "addition", "the", "four", "C", "delta", "exons", "were", "found", "to", "be", "already", "assembled", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14154
[ "Two", "polyadenylation", "sites", "were", "present", "in", "the", "fourth", "C", "delta", "exon", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14155
[ "However", ",", "only", "that", "located", "at", "the", "3", "'", "end", "appeared", "to", "be", "utilized", "in", "the", "2.3-kb", "cDNA", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14156
[ "The", "expression", "of", "hGATA-3", ",", "a", "T", "cell-specific", "factor", "known", "to", "be", "involved", "in", "the", "regulation", "of", "the", "transcription", "of", "TcR", "delta", "locus", ",", "was", "analyzed", "by", "Northern", "blot", ",", "in", "cultured", "NK", "cell", "population", "and", "clones", "(", "but", "not", "in", "freshly", "derived", "cell", "populations", ")", "." ]
[ 0, 0, 0, 9, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14157
[ "All", "NK", "clones", "and", "cell", "lines", "studied", "were", "found", "to", "express", "hGATA-3-specific", "mRNA", ",", "suggesting", "that", "hGATA-3", "may", "be", "involved", "in", "the", "regulation", "of", "the", "unrearranged", "TcR", "delta", "gene", "expression", "in", "NK", "cells", "." ]
[ 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 7, 8, 0 ]
14158
[ "Finally", ",", "no", "transcription", "of", "the", "RAG-1", "gene", "could", "be", "detected", "in", "all", "NK", "cell", "lines", "or", "clones", "analyzed", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0 ]
14159
[ "Negative", "transcriptional", "regulation", "of", "human", "interleukin", "2", "(", "IL-2", ")", "gene", "by", "glucocorticoids", "through", "interference", "with", "nuclear", "transcription", "factors", "AP-1", "and", "NF-AT", "." ]
[ 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0 ]
14160
[ "IL-2", "gene", "transcription", "is", "affected", "by", "several", "nuclear", "proteins", "." ]
[ 1, 2, 0, 0, 0, 0, 0, 0, 0, 0 ]
14161
[ "We", "asked", "whether", "dexamethasone", "(", "Dex", ")", "and", "cyclosporin", "A", "(", "CsA", ")", "inhibit", "IL-2", "gene", "transcription", "by", "interfering", "with", "the", "activity", "of", "nuclear", "proteins", "that", "bind", "to", "the", "IL-2", "promoter", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]
14162
[ "Nuclear", "extracts", "from", "primary", "human", "T", "lymphocytes", "were", "analyzed", "by", "electrophoretic", "DNA", "mobility", "shift", "assays", "." ]
[ 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14163
[ "Both", "Dex", "and", "CsA", "inhibited", "the", "binding", "of", "transcription", "factors", "AP-1", "and", "NF-AT", ",", "but", "not", "of", "NF-kB", "and", "OCT-1/OAF", ",", "to", "their", "corresponding", "sites", "on", "the", "IL-2", "gene", "promoter", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0 ]
14164
[ "To", "correlate", "changes", "in", "nuclear", "factor", "binding", "in", "vitro", "with", "transcriptional", "activity", "in", "vivo", "and", "define", "the", "structural", "requirements", "for", "IL-2", "promoter", "repression", ",", "we", "used", "transient", "DNA", "transfections", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0 ]
14165
[ "Jurkat", "cells", "were", "transfected", "with", "plasmids", "containing", "either", "the", "intact", "IL-2", "promoter", "or", "its", "AP-1", ",", "NF-AT", ",", "and", "NF-kB", "motifs", "." ]
[ 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 0, 1, 0, 0, 1, 2, 0 ]
14166
[ "Dex", "inhibited", "the", "IL-2", "promoter", "and", "the", "AP-1", ",", "but", "not", "the", "NF-AT", "and", "NF-kB", "plasmids", "." ]
[ 0, 0, 0, 1, 2, 0, 0, 1, 0, 0, 0, 0, 1, 0, 1, 2, 0 ]
14167
[ "In", "contrast", ",", "CsA", "inhibited", "the", "IL-2", "promoter", "and", "the", "NF-AT", ",", "but", "not", "the", "AP-1", "and", "NF-kB", "plasmids", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 0, 0, 0, 0, 1, 0, 1, 2, 0 ]
14168
[ "These", "results", "suggest", "that", "in", "human", "T", "lymphocytes", "both", "Dex", "and", "CsA", "inhibited", "IL-2", "gene", "transcription", "through", "interference", "with", "transcription", "factors", "AP-1", "and", "NF-AT", "." ]
[ 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0 ]
14169
[ "We", "propose", "that", ",", "while", "maximum", "inhibition", "may", "involve", "interaction", "with", "both", "transcription", "factors", ",", "AP-1", "is", "the", "primary", "target", "of", "Dex", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]
14170
[ "Nuclear", "factor", "kappa", "B", ",", "a", "mediator", "of", "lipopolysaccharide", "effects", "." ]
[ 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0 ]
14171
[ "Exposure", "of", "certain", "cell", "types", "to", "bacterial", "lipopolysaccharide", "(", "LPS", ")", "leads", "to", "activation", "of", "nuclear", "factor", "kappa", "B", "(", "NF-kappa", "B", ")", ",", "an", "inducible", "transcription", "factor", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0 ]
14172
[ "One", "of", "NF-kappa", "B", "'s", "unique", "properties", "is", "its", "posttranslational", "activation", "via", "release", "of", "an", "inhibitory", "subunit", ",", "called", "inhibitor", "of", "NF-kappa", "B", "(", "I", "kappa", "B", ")", ",", "from", "a", "sequestered", "cytoplasmic", "form", "." ]
[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0 ]
14173
[ "This", "event", "is", "also", "triggered", "under", "various", "other", "conditions", "of", "biomedical", "importance", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14174
[ "Other", "bacterial", "toxins", ",", "tumor", "necrosis", "factor-alpha", "(", "TNF", ")", ",", "interleukin-1", "(", "IL-1", ")", ",", "T", "cell", "mitogens", ",", "UV", "light", ",", "gamma", "rays", "and", "oxidative", "stress", "were", "reported", "to", "induce", "NF-kappa", "B", "." ]
[ 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 9, 0, 9, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]
14175
[ "The", "activated", "form", "of", "NF-kappa", "B", ",", "which", "is", "rapidly", "taken", "up", "into", "nuclei", ",", "initiates", "transcription", "from", "immediate", "early", "genes", "in", "a", "wide", "variety", "of", "cell", "types", "." ]
[ 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14176
[ "Most", "of", "the", "target", "genes", "for", "NF-kappa", "B", "are", "of", "relevance", "for", "the", "immune", "response", "and", "can", "be", "grouped", "into", "those", "encoding", "cytokines", ",", "cell", "surface", "receptors", ",", "acute", "phase", "proteins", "and", "viral", "genomes", ",", "such", "as", "that", "of", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14177
[ "We", "will", "discuss", "recent", "experimental", "evidences", "suggesting", "that", "LPS", "might", "share", "a", "pathway", "of", "NF-kappa", "B", "activation", "with", "other", "inducers", "of", "the", "factor", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0 ]
14178
[ "This", "common", "pathway", "may", "involve", "reactive", "oxygen", "intermediates", "(", "ROI", ")", "as", "messenger", "molecules", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14179
[ "[", "The", "trend", "of", "molecular", "biology", "study", "on", "eosinophils", "]" ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 7, 0 ]
14180
[ "Recently", ",", "many", "investigators", "have", "been", "interested", "in", "the", "study", "on", "eosinophil", "biology", "since", "genes", "association", "with", "eosinophils", "such", "as", "interleukin-5", "or", "eosinophil", "granule", "proteins", "(", "EPO", ",", "ECP", ",", "EDN", ",", "MBP", ",", "and", "CLC", ")", ",", "were", "isolated", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 7, 0, 0, 9, 0, 7, 0, 0, 0, 9, 0, 9, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0 ]
14181
[ "However", ",", "the", "molecular", "basis", "for", "the", "commitment", "of", "progenitors", "to", "the", "eosinophil", "lineage", "has", "not", "been", "determined", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0 ]
14182
[ "The", "mechanism", "by", "which", "eosinophil-specific", "genes", "encoding", "primary", "and", "secondary", "granule", "proteins", "(", "e.g", ".", "ECP", ",", "EDN", ",", "EPO", ",", "MBP", ",", "and", "CLC", ")", "are", "expressed", "and", "regulated", "during", "eosinophilopoiesis", "is", "also", "unknown", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14183
[ "In", "this", "paper", ",", "I", "described", "the", "characterization", "of", "genes", "encoding", "eosinophil", "granule", "proteins", "and", "the", "mRNA", "expression", "of", "GATA-1", "binding", "transcription", "factor", "during", "eosinophil", "differentiation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 7, 0, 0 ]
14184
[ "Characterization", "of", "the", "nuclear", "and", "cytoplasmic", "components", "of", "the", "lymphoid-specific", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NF-AT", ")", "complex", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 10, 0 ]
14185
[ "The", "lymphoid-specific", "transcription", "complex", ",", "NF-AT", ",", "is", "involved", "in", "early", "gene", "activation", "in", "T", "cells", "and", "is", "assembled", "from", "a", "pre-existing", ",", "T", "cell", "restricted", "cytoplasmic", "factor", "and", "an", "inducible", "ubiquitous", "nuclear", "component", "within", "30", "min", "after", "activation", "through", "the", "antigen", "receptor", "." ]
[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14186
[ "Recent", "studies", "have", "implicated", "the", "family", "of", "AP1", "factors", "as", "components", "of", "the", "murine", "NF-AT", "complex", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 10, 0 ]
14187
[ "Evidence", "is", "provided", "here", "that", "the", "nuclear", "component", "of", "human", "NF-AT", "contains", "the", "phorbol", "ester-inducible", "transcription", "factor", "AP1", "(", "Jun/Fos", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0 ]
14188
[ "We", "further", "characterize", "which", "AP1", "family", "members", "can", "assume", "this", "role", "." ]
[ 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0 ]
14189
[ "Antisera", "to", "Fos", "inhibits", "NF-AT", "DNA", "binding", "as", "does", "an", "oligonucleotide", "containing", "a", "binding", "site", "for", "AP1", "." ]
[ 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
14190
[ "Constitutive", "expression", "in", "vivo", "of", "Fos", ",", "and", "to", "a", "lesser", "extent", "Fra-1", ",", "eliminates", "the", "requirement", "for", "phorbol", "12-myristate", "13-acetate", "(", "PMA", ")", "stimulation", ",", "leaving", "NF-AT", "-directed", "transcription", "responsive", "to", "calcium", "ionophore", "alone", "." ]
[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]
14191
[ "Overexpression", "of", "cJun", "or", "JunD", ",", "but", "not", "JunB", ",", "also", "eliminates", "the", "requirement", "for", "PMA", ",", "indicating", "that", "many", "but", "not", "all", "Jun-", "and", "Fos-related", "proteins", "functionally", "activate", "NF-AT", "-dependent", "transcription", "in", "the", "presence", "of", "the", "cytoplasmic", "component", "." ]
[ 0, 0, 9, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14192
[ "NF-AT", "DNA", "binding", "can", "be", "reconstituted", "in", "vitro", "using", "semi-purified", "AP1", "proteins", "mixed", "with", "cytosol", "from", "T", "lymphocytes", "." ]
[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 7, 8, 0 ]
14193
[ "Fos", "proteins", "are", "not", "needed", "for", "this", "reconstitution", ",", "and", "although", "JunB", "is", "not", "functional", ",", "it", "can", "participate", "in", "the", "NF-AT", "DNA", "binding", "complex", "." ]
[ 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]
14194
[ "Finally", ",", "we", "have", "partially", "purified", "the", "cytoplasmic", "component", "of", "NF-AT", "and", "show", "by", "elution", "and", "renaturation", "from", "SDS-polyacrylamide", "gel", "electrophoresis", "gels", "that", "it", "has", "a", "molecular", "mass", "between", "94", "and", "116", "kDa", "and", "may", "have", "multiple", "differentially", "modified", "forms", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14195
[ "Glucocorticoid", "receptors", "in", "mononuclear", "cells", "of", "patients", "with", "sepsis", "." ]
[ 9, 10, 0, 7, 8, 0, 0, 0, 0, 0 ]
14196
[ "Glucocorticoid", "receptor", "(", "GR", ")", "hormone-binding", "activity", "was", "studied", "by", "a", "whole-cell", "method", "in", "mononuclear", "cells", "(", "MNC", ")", "from", "peripheral", "blood", "of", "7", "patients", "during", "the", "hemodynamic", "compensatory", "phase", "of", "sepsis", "." ]
[ 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14197
[ "4", "patients", "were", "receiving", "dopamine", ",", "which", "did", "not", "affect", "the", "GR", "count", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]
14198
[ "The", "patients", "'", "plasma", "cortisol", "concentrations", "were", "normal", "or", "slightly", "elevated", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14199
[ "Despite", "a", "wide", "range", ",", "the", "mean", "GR", "count", "and", "affinity", "in", "MNC", "from", "septic", "patients", "did", "not", "differ", "from", "those", "in", "normal", "controls", ",", "suggesting", "that", "glucocorticoids", "could", "still", "be", "effective", "in", "the", "hemodynamic", "compensatory", "phase", "of", "sepsis", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
14200
[ "The", "impaired", "transcription", "factor", "AP-1", "DNA", "binding", "activity", "in", "lymphocytes", "derived", "from", "subjects", "with", "some", "symptoms", "of", "premature", "aging", "." ]
[ 0, 0, 9, 10, 10, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]