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11701
[ "In", "addition", "to", "the", "existing", "isoform", "(", "Pax-5", ",", "which", "we", "have", "named", "Pax-5a", ")", ",", "we", "have", "isolated", "three", "new", "isoforms", ",", "Pax-5b", ",", "Pax-5d", ",", "and", "Pax-5e", ",", "from", "murine", "spleen", "and", "B-lymphoid", "cell", "lines", "using", "library", "screenings", "and", "polymerase", "chain", "reaction", "amplification", "." ]
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11702
[ "Isoforms", "Pax-5b", "and", "Pax-5e", "have", "spliced", "out", "their", "second", "exon", ",", "resulting", "in", "proteins", "with", "only", "a", "partial", "DNA-binding", "domain", "." ]
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11703
[ "Isoforms", "Pax-5d", "and", "Pax-5e", "have", "deleted", "the", "3'-region", ",", "which", "encodes", "the", "transactivating", "domain", ",", "and", "replaced", "it", "with", "a", "novel", "sequence", "." ]
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11704
[ "The", "existence", "of", "alternative", "Pax-5", "transcripts", "was", "confirmed", "using", "RNase", "protection", "assays", "." ]
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11705
[ "Furthermore", ",", "Pax-5a", "and", "Pax-5b", "proteins", "were", "detected", "using", "Western", "blot", "analysis", "." ]
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11706
[ "Pax-5a", "was", "detectable", "in", "pro-", ",", "pre-", ",", "and", "mature", "B-cell", "lines", ",", "but", "not", "in", "two", "plasmacytomas", ";", "Pax-5b", "was", "shown", "to", "be", "present", "at", "low", "levels", "in", "mature", "B-cell", "lines", "and", ",", "unexpectedly", ",", "in", "one", "plasma", "cell", "line", ",", "but", "not", "in", "pro-B-cell", "or", "T-cell", "lines", "." ]
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11707
[ "Mobility", "shift", "assays", "showed", "that", "in", "vitro", "translated", "Pax-5a", "and", "Pax-5d", ",", "but", "not", "Pax-5b", "or", "Pax-5e", ",", "could", "interact", "with", "a", "B-cell-specific", "activator", "protein-binding", "site", "on", "the", "blk", "promoter", "." ]
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11708
[ "Using", "this", "assay", ",", "we", "also", "showed", "that", "Pax-5d", "was", "present", "in", "nuclear", "extracts", "of", "some", "(", "but", "not", "all", ")", "B-lymphoid", "lines", "and", "interacts", "with", "the", "B-cell-specific", "activator", "protein-binding", "site", "." ]
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11709
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11710
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11711
[ "The", "two", "related", "protein-tyrosine", "kinases", "Syk", "and", "Zap", "are", "rapidly", "phosphorylated", "on", "tyrosine", "residues", "and", "enzymatically", "activated", "upon", "crosslinking", "of", "the", "T", "cell", "antigen", "receptor", "." ]
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11712
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11713
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11714
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11715
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11716
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11717
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11718
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11719
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11720
[ "Activation", "of", "the", "transcription", "factor", "MEF2C", "by", "the", "MAP", "kinase", "p38", "in", "inflammation", "." ]
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11721
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11722
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11723
[ "In", "mammalian", "cells", "the", "MAPK", "p38", "is", "thought", "to", "play", "an", "important", "role", "in", "the", "regulation", "of", "cellular", "responses", "during", "infection", "through", "its", "effects", "on", "the", "expression", "of", "proinflammatory", "molecules", "." ]
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11724
[ "One", "means", "of", "understanding", "the", "role", "of", "p38", "in", "these", "responses", "is", "to", "identify", "proteins", "with", "functions", "regulated", "by", "p38", "-catalysed", "phosphorylation", "." ]
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11725
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11726
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11727
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11728
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11729
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11730
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11731
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11732
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11733
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11734
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11735
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11736
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11737
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11738
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11739
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11740
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11741
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11742
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11743
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11744
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11746
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11748
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11750
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11751
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11754
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