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11801
[ "In", "this", "report", ",", "we", "describe", "the", "cloning", "of", "human", "GATA-4", "(", "hGATA-4", ")", "and", "show", "that", "hGATA-4", "selectively", "interacts", "with", "the", "-70", "GATA", "site", "within", "the", "IL-5", "proximal", "promoter", "region", "." ]
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11802
[ "By", "promoter", "deletion", "and", "mutation", "analyses", ",", "we", "established", "this", "region", "as", "a", "positive", "regulatory", "element", "." ]
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11803
[ "Cotransfection", "experiments", "revealed", "that", "both", "hGATA-4", "and", "PMA/A23187", "stimulation", "are", "necessary", "for", "the", "IL-5", "promoter", "activation", "." ]
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11804
[ "The", "requirement", "of", "another", "regulatory", "element", "called", "CLE0", ",", "which", "lies", "downstream", "of", "the", "-70", "GATA", "site", ",", "was", "also", "demonstrated", "." ]
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11805
[ "ATL-16T", "cells", "express", "mRNA", "of", "three", "GATA-binding", "proteins", ",", "hGATA-2", ",", "hGATA-3", "and", "hGATA-4", ",", "and", "each", "of", "them", "has", "a", "potential", "to", "bind", "to", "the", "consensus", "(", "A/T", ")", "GATA", "(", "G/", "A", ")", "motif", "." ]
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11806
[ "However", ",", "using", "ATL-16T", "nuclear", "extract", ",", "we", "demonstrated", "that", "GATA-4", "is", "the", "only", "GATA-binding", "protein", "that", "forms", "specific", "DNA-protein", "complex", "with", "the", "-70", "GATA", "site", "." ]
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11807
[ "The", "electrophoretic", "mobility", "shift", "assay", "with", "extracts", "of", "COS", "cells", "expressing", "GATA-binding", "proteins", "showed", "that", "GATA-4", "has", "the", "highest", "binding", "affinity", "to", "the", "-70", "GATA", "site", "among", "the", "three", "GATA-binding", "proteins", "." ]
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11808
[ "When", "the", "transactivation", "ability", "was", "compared", "among", "the", "three", ",", "GATA-4", "showed", "the", "highest", "activity", "." ]
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11809
[ "These", "results", "demonstrate", "the", "selective", "role", "of", "GATA-4", "in", "the", "transcriptional", "regulation", "of", "the", "IL-5", "gene", "in", "a", "circumstance", "where", "multiple", "members", "of", "the", "GATA-binding", "proteins", "are", "expressed", "." ]
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11810
[ "Control", "of", "NFATx1", "nuclear", "translocation", "by", "a", "calcineurin-regulated", "inhibitory", "domain", "." ]
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11811
[ "The", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NFAT", ")", "regulates", "cytokine", "gene", "expression", "in", "T", "cells", "through", "cis-acting", "elements", "located", "in", "the", "promoters", "of", "several", "cytokine", "genes", "." ]
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11812
[ "NFATx1", ",", "which", "is", "preferentially", "expressed", "in", "the", "thymus", "and", "peripheral", "blood", "leukocytes", ",", "is", "one", "of", "four", "members", "of", "the", "NFAT", "family", "of", "transcription", "factors", "." ]
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11813
[ "We", "have", "performed", "domain", "analysis", "of", "NFATx1", "by", "examining", "the", "effects", "of", "deletion", "mutations", "." ]
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11814
[ "We", "found", "that", "NFATx1", "DNA", "binding", "activity", "and", "interaction", "with", "AP-1", "polypeptides", "were", "dependent", "on", "its", "central", "Rel", "similarity", "region", "and", "that", "transcriptional", "activation", "was", "reduced", "by", "deletions", "of", "either", "its", "N-terminal", "domain", "or", "its", "C-terminal", "domain", ",", "suggesting", "the", "presence", "of", "intrinsic", "transcriptional", "activation", "motifs", "in", "both", "regions", "." ]
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11815
[ "We", "also", "identified", "a", "potent", "inhibitory", "sequence", "within", "its", "N-terminal", "domain", "." ]
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11816
[ "We", "show", "that", "the", "inactivation", "of", "the", "inhibition", "was", "dependent", "on", "the", "activity", "of", "calcineurin", ",", "a", "calcium-calmodulin-dependent", "phosphatase", "." ]
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11817
[ "We", "also", "show", "that", "calcineurin", "associated", "with", "the", "N-terminal", "domain", "of", "NFATx1", "at", "multiple", "docking", "sites", "and", "caused", "a", "reduction", "of", "size", ",", "indicative", "of", "dephosphorylation", ",", "in", "NFATx1", "." ]
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11818
[ "We", "have", "mapped", "the", "inhibitory", "activity", "to", "less", "than", "60", "residues", ",", "containing", "motifs", "that", "are", "conserved", "in", "all", "NFAT", "proteins", "." ]
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11819
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11820
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11821
[ "Neuronal", "(", "type", "I", ")", "nitric", "oxide", "synthase", "regulates", "nuclear", "factor", "kappaB", "activity", "and", "immunologic", "(", "type", "II", ")", "nitric", "oxide", "synthase", "expression", "." ]
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11822
[ "Nitric", "oxide", "subserves", "diverse", "physiologic", "roles", "in", "the", "nervous", "system", "." ]
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11823
[ "NO", "is", "produced", "from", "at", "least", "three", "different", "NO", "synthase", "(", "NOS", ")", "isoforms", ":", "neuronal", "NOS", "(", "nNOS", ")", ",", "endothelial", "NOS", ",", "and", "immunologic", "NOS", "(", "iNOS", ")", "." ]
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11824
[ "We", "show", "that", "nNOS", "is", "the", "predominant", "isoform", "constitutively", "expressed", "in", "glia", "." ]
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11825
[ "NO", "derived", "from", "nNOS", "in", "glia", "inhibits", "the", "transcription", "factor", "nuclear", "factor", "kappaB", "(", "NF", "kappaB", ")", "as", "NOS", "inhibitors", "enhance", "basal", "NF", "kappaB", "activation", "." ]
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11826
[ "Pyrrolidine", "dithiocarbamate", "(", "PDTC", ")", "is", "an", "inhibitor", "of", "NF", "kappaB", "in", "most", "cells", ";", "however", ",", "we", "show", "that", "PDTC", "is", "also", "a", "potent", "scavenger", "of", "NO", "through", "formation", "of", "mononitrosyl", "iron", "complexes", "with", "PDTC", "." ]
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11827
[ "In", "Jurkat", "cells", ",", "a", "human", "T-cell", "lymphoma", "cell", "line", ",", "tumor", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", "induces", "NF", "kappaB", "activation", "that", "is", "inhibited", "by", "PDTC", "." ]
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11828
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11829
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11830
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11831
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11832
[ "Involvement", "of", "Stat3", "in", "interleukin-6", "-induced", "IgM", "production", "in", "a", "human", "B-cell", "line", "." ]
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11833
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11834
[ "IL-6", "treatment", "of", "the", "human", "lymphoblastoid", "cell", "line", ",", "SKW6.4", ",", "leads", "to", "increased", "IgM", "production", "." ]
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11835
[ "We", "have", "previously", "shown", "that", "IL-6", "induces", "activation", "of", "JAK1", "and", "JAK2", "in", "human", "B", "cell", "lines", "." ]
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11836
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11837
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11838
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11839
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11840
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11841
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11842
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11843
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11844
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11845
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11846
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11847
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11848
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11849
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11850
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11851
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11852
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11853
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11854
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11855
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11856
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11857
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11858
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11859
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11860
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11861
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11862
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11863
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11864
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11865
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11867
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11868
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11869
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11870
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11871
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11872
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11873
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11874
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11875
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11876
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11877
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11878
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11879
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11880
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11881
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11882
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11883
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11884
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11885
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11886
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11887
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11888
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11889
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11890
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11891
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11892
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11893
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11894
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11895
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11896
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11897
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11898
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11899
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