Datasets:

---

siddharthtumre

/

Revised-JNLPBA

like 0

[ "Our", "results", "demonstrate", "the", "regulation", "of", "STAT", "activity", "by", "a", "signaling", "pathway", "that", "regulates", "the", "T", "cell", "functional", "phenotype", "and", "is", "distinct", "from", "the", "cytokine", "-activated", "Janus", "kinase", "-STAT", "signaling", "pathway", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0 ]

[ "Suppression", "of", "c-jun", "by", "antisense", "oligonucleotides", "inhibits", "cell", "adhesion", "but", "not", "respiratory", "burst", "during", "phorbol", "ester-induced", "differentiation", "of", "U937", "human", "monoblastic", "cells", "." ]

[ 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0 ]

[ "We", "studied", "the", "role", "of", "the", "immediate", "early", "gene", "c-jun", "in", "cell", "proliferation", "and", "phorbol", "12-myristate", "13-acetate", "(", "PMA", ")", "-induced", "differentiation", "in", "U937", "human", "monoblastic", "cells", ",", "using", "c-jun", "-specific", "antisense", "(", "AS", ")", "phosphorothioate", "oligonucleotides", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "selecting", "the", "most", "specific", "and", "potent", "oligonucleotide", "sequence", ",", "we", "performed", "extensive", "analyses", "for", "the", "binding", "specificity", "between", "all", "candidates", "of", "c-jun", "AS", "oligonucleotides", "and", "the", "whole", "sequences", "in", "GenBank", "database", ",", "using", "a", "computer", "program", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Among", "the", "20", "selected", "oligonucleotides", ",", "two", "potent", "15-mer", "AS", "oligonucleotides", "(", "C-JUN", "AS", "oligonucleotides", ")", "exhibited", "significant", "inhibition", "of", "cell", "growth", "in", "a", "dose-dependent", "manner", "between", "2", "and", "10", "microM", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Reverse", "transcription-PCR", "and", "Western", "blot", "analysis", "demonstrated", "that", "10", "microM", "of", "C-JUN", "AS", "oligonucleotides", "reduced", "c-jun", "expression", "at", "both", "the", "mRNA", "and", "protein", "levels", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "More", "importantly", ",", "C-JUN", "AS", "oligonucleotides", "showed", "distinct", "effects", "on", "two", "markers", "of", "PMA-induced", "differentiation", ";", "the", "C-JUN", "AS", "oligonucleotides", "inhibited", "cell", "adhesion", ",", "whereas", "they", "did", "not", "affect", "another", "marker", "of", "differentiation", ",", "respiratory", "burst", "(", "measured", "by", "nitro", "blue", "tetrazolium", "reduction", "assay", ")", "." ]

[ 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "These", "results", "suggest", "a", "critical", "role", "of", "c-jun", "in", "both", "cell", "proliferation", "and", "PMA-induced", "cell", "adhesion", "but", "not", "in", "PMA-induced", "respiratory", "burst", "in", "U937", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]

[ "Effects", "of", "IL-10", "and", "IL-4", "on", "LPS-induced", "transcription", "factors", "(", "AP-1", ",", "NF-IL6", "and", "NF-kappa", "B", ")", "which", "are", "involved", "in", "IL-6", "regulation", "." ]

[ 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 9, 0, 9, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "Interleukin-10", "(", "IL-10", ")", ",", "like", "IL-4", ",", "is", "known", "to", "inhibit", "cytokine", "expression", "in", "activated", "human", "monocytes", "." ]

[ 9, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "We", "showed", "that", "both", "IL-10", "and", "IL-4", "inhibit", "LPS-induced", "IL-6", "mRNA", "and", "protein", "expression", "by", "inhibiting", "the", "transcription", "rate", "of", "the", "IL-6", "gene", "." ]

[ 0, 0, 0, 0, 9, 0, 9, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]

[ "The", "strong", "inhibition", "of", "the", "IL-6", "transcription", "rate", "prompted", "us", "to", "study", "the", "effect", "of", "IL-10", "and", "IL-4", "on", "the", "expression", "of", "transcription", "factors", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "questioned", "whether", "or", "not", "IL-10", "and", "IL-4", "affected", "the", "expression", "of", "transcription", "factors", "that", "are", "known", "to", "be", "involved", "in", "the", "control", "of", "the", "IL-6", "transcription", "rate", ",", "namely", "activator", "protein-1", "(", "AP-1", ")", ",", "nuclear", "factor", "IL-6", "(", "NF-IL6", ")", ",", "and", "nuclear", "factor", "kappa", "B", "(", "NF-kappaB", ")", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0 ]

[ "In", "electrophoretic", "mobility", "shift", "assays", "(", "EMSAs", ")", "we", "showed", "that", "IL-10", "and", "IL-4", "inhibited", "LPS-induced", "AP-1", "binding", "activity", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0 ]

[ "The", "inhibiting", "effect", "of", "IL-4", "was", "slightly", "more", "pronounced", "than", "that", "of", "IL-10", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "Downregulation", "of", "LPS-induced", "AP-1", "was", "accompanied", ",", "and", "thus", "possibly", "explained", ",", "by", "a", "reduced", "expression", "at", "mRNA", "level", "of", "the", "two", "major", "components", "of", "the", "AP-1", "complex", ",", "namely", "c-fos", "and", "c-jun", "as", "determined", "by", "Northern", "experiments", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0 ]

[ "Binding", "activity", "of", "NF-IL6", "was", "also", "strongly", "inhibited", "by", "IL-4", "whereas", "IL-10", "showed", "no", "effect", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0 ]

[ "NF-IL6", "mRNA", "levels", "were", "not", "affected", "by", "IL-10", "or", "IL-4", ",", "suggesting", "that", "IL-4", "affects", "binding", "activity", "of", "preexisting", "NF-IL6", "." ]

[ 3, 4, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0 ]

[ "Neither", "IL-10", "nor", "IL-4", "inhibited", "LPS-induced", "NF-kappa", "B", "binding", "activity", "." ]

[ 0, 9, 0, 9, 0, 0, 9, 10, 0, 0, 0 ]

[ "In", "agreement", "with", "this", "finding", ",", "Northern", "experiments", "where", "p65", "and", "p105", "mRNA", "levels", "were", "determined", ",", "demonstrated", "that", "expression", "of", "these", "components", "of", "the", "NF-kappa", "B", "transcription", "factor", "were", "not", "affected", "by", "IL-10", "or", "IL-4", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 9, 0, 9, 0 ]

[ "Furthermore", ",", "neither", "IL-10", "nor", "IL-4", "showed", "any", "effect", "on", "I-kappa", "B", "mRNA", "expression", "as", "determined", "by", "Northern", "experiments", "." ]

[ 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 3, 4, 4, 0, 0, 0, 0, 0, 0, 0 ]

[ "Thus", ",", "IL-10", "and", "IL-4", "similarly", "affect", "IL-6", "expression", "." ]

[ 0, 0, 9, 0, 9, 0, 0, 9, 0, 0 ]

[ "However", ",", "for", "IL-4", "this", "was", "accompanied", "with", "a", "reduction", "of", "AP-1", "and", "NF-IL6", "binding", "activity", "whereas", "IL-10", "only", "inhibited", "AP-1", "binding", "activity", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 0, 0, 9, 0, 0, 0 ]

[ "Regulation", "of", "GM-CSF", "gene", "transcription", "by", "core-binding", "factor", "." ]

[ 0, 0, 1, 2, 0, 0, 9, 10, 0 ]

[ "GM-CSF", "gene", "activation", "in", "T", "cells", "is", "known", "to", "involve", "the", "transcription", "factors", "nuclear", "factor-kappa", "B", ",", "AP-1", ",", "NFAT", ",", "and", "Sp1", "." ]

[ 1, 2, 0, 0, 7, 8, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 0, 9, 0, 0, 9, 0 ]

[ "Here", "we", "demonstrate", "that", "the", "human", "GM-CSF", "promoter", "and", "enhancer", "also", "encompass", "binding", "sites", "for", "core-binding", "factor", "(", "CBF", ")", "." ]

[ 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0 ]

[ "Significantly", ",", "the", "CBF", "sites", "are", "in", "each", "case", "contained", "within", "the", "minimum", "essential", "core", "regions", "required", "for", "inducible", "activation", "of", "transcription", "." ]

[ 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Furthermore", ",", "these", "core", "regions", "of", "the", "enhancer", "and", "promoter", "each", "encompass", "closely", "linked", "binding", "sites", "for", "CBF", ",", "AP-1", ",", "and", "NFATp", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0 ]

[ "The", "GM-CSF", "promoter", "CBF", "site", "TGTGGTCA", "is", "located", "51", "bp", "upstream", "of", "the", "transcription", "start", "site", "and", "also", "overlaps", "a", "YY-1", "binding", "site", "." ]

[ 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0 ]

[ "A", "2-bp", "mutation", "within", "the", "CBF", "site", "resulted", "in", "a", "2-3-fold", "decrease", "in", "the", "activities", "of", "both", "a", "69-bp", "proximal", "promoter", "fragment", "and", "a", "627-bp", "full-length", "promoter", "fragment", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Stepwise", "deletions", "into", "the", "proximal", "promoter", "also", "revealed", "that", "the", "CBF", "site", ",", "but", "not", "the", "YY-1", "site", ",", "was", "required", "for", "efficient", "induction", "of", "transcriptional", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "AML1", "and", "CBF", "beta", "genes", "that", "encode", "CBF", "each", "have", "the", "ability", "to", "influence", "cell", "growth", "and", "differentiation", "and", "have", "been", "implicated", "as", "proto-oncogenes", "in", "acute", "myeloid", "leukemia", "." ]

[ 0, 1, 0, 1, 2, 2, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "This", "study", "adds", "GM-CSF", "to", "a", "growing", "list", "of", "cytokines", "and", "receptors", "that", "are", "regulated", "by", "CBF", "and", "which", "control", "the", "growth", ",", "differentiation", ",", "and", "activation", "of", "hemopoietic", "cells", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "The", "GM-CSF", "locus", "may", "represent", "one", "of", "several", "target", "genes", "that", "are", "dysregulated", "in", "acute", "myeloid", "leukemia", "." ]

[ 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Sublethal", "levels", "of", "oxidative", "stress", "stimulate", "transcriptional", "activation", "of", "c-jun", "and", "suppress", "IL-2", "promoter", "activation", "in", "Jurkat", "T", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 1, 2, 0, 0, 5, 6, 6, 0 ]

[ "Sublethal", "levels", "of", "oxidative", "stress", "are", "well", "known", "to", "alter", "T", "cell", "functional", "responses", ",", "but", "the", "underlying", "mechanisms", "are", "unknown", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "current", "study", "examined", "the", "effects", "of", "oxidative", "stress", "on", "transcriptional", "activities", "mediated", "by", "c-Fos/c-Jun", "AP-1", "and", "the", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NF-AT", ")", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 9, 10, 10, 10, 10, 10, 0, 9, 0, 0 ]

[ "The", "present", "results", "show", "that", "Jurkat", "T", "cells", "acutely", "exposed", "to", "micromolar", "concentrations", "of", "H2O2", "exhibit", "substantial", "increases", "in", "AP-1", "binding", "activity", "and", "the", "expression", "of", "c-jun", "but", "not", "c-fos", "mRNA", "." ]

[ 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 3, 0, 0, 3, 4, 0 ]

[ "The", "preferential", "induction", "of", "c-jun", "by", "H2O2", "did", "not", "represent", "redox", "stabilization", "of", "mRNA", "transcripts", ",", "and", "oxidative", "signals", "closely", "resembled", "PHA/PMA", "stimulation", "by", "effectively", "transactivating", "the", "full", "length", "c-jun", "promoter", "via", "the", "proximal", "jun1", "tumor", "promoter-responsive", "element", "(", "TRE", ")", "-like", "promoter", "element", "." ]

[ 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0 ]

[ "Similarly", ",", "the", "complexes", "binding", "the", "consensus", "AP-1", "TRE", "and", "jun", "TRE-like", "motifs", "in", "cells", "exposed", "to", "oxidative", "signals", "or", "PHA/PMA", "were", "indistinguishable", ",", "being", "composed", "of", "c-Fos", ",", "c-Jun", ",", "and", "JunD", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0 ]

[ "However", ",", "PHA/PMA", "but", "not", "oxidative", "signals", "induced", "the", "coordinate", "activation", "of", "reporter", "constructs", "containing", "the", "AP-1-TRE", ",", "NF-AT", ",", "and", "IL-2", "promoter", "regions", "along", "with", "IL-2", "mRNA", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 1, 2, 0, 0, 0, 3, 4, 0, 0 ]

[ "Furthermore", ",", "sublethal", "levels", "of", "H2O2", "actively", "suppressed", "the", "transcriptional", "activation", "of", "NF-AT", "and", "IL-2", "reporters", "as", "well", "as", "the", "expression", "of", "IL-2", "mRNA", "in", "cells", "stimulated", "with", "PHA/PMA", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0 ]

[ "Gel", "shift", "analysis", "revealed", "that", "oxidative", "suppression", "of", "NF-AT", "represented", "inhibition", "in", "the", "early", "generation", "of", "NFAT", "complexes", "rather", "than", "the", "binding", "of", "preformed", "NF-AT", "complexes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "These", "results", "suggest", "that", "oxidative", "signals", "can", "positively", "and", "negatively", "regulate", "T", "cell", "transcriptional", "events", "and", "that", "changes", "in", "cellular", "redox", "can", "uncouple", "AP-1", "regulation", "of", "c-jun", "from", "transcriptional", "up-regulation", "of", "IL-2", "via", "NF-AT", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0, 0, 0, 0, 9, 0, 9, 0 ]

[ "Inorganic", "lead", "activates", "NF-kappa", "B", "in", "primary", "human", "CD4+", "T", "lymphocytes", "." ]

[ 0, 0, 0, 9, 10, 0, 0, 7, 8, 8, 8, 0 ]

[ "Inorganic", "lead", "(", "Pb", ")", "is", "a", "ubiquitous", "environmental", "contaminant", "that", "produces", "a", "variety", "of", "effects", "on", "humoral", "and", "cell", "mediated", "immune", "responses", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "underlying", "molecular", "mechanism", "for", "Pb", "'s", "complex", "effects", "on", "the", "immune", "system", "remain", "obscure", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Many", "of", "Pb", "'s", "effects", "on", "the", "immune", "system", "could", "be", "explained", "through", "activation", "of", "the", "transcription", "factor", ",", "NF-kappa", "B", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "NF-kappa", "B", "is", "critical", "for", "T", "lymphocyte", "function", "and", "is", "a", "strong", "inducer", "of", "HIV-LTR", "activation", "." ]

[ 9, 10, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0 ]

[ "We", "demonstrate", "that", "Pb", "at", "physiologically", "relevant", "concentrations", "activates", "NF-kappa", "B", "in", "primary", "human", "CD4+", "T", "lymphocytes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 7, 8, 8, 8, 0 ]

[ "Pb-induced", "activation", "of", "NF-kappa", "B", "is", "blocked", "by", "antibodies", "for", "p65", "and", "p50", "subunits", "but", "not", "cRel", ",", "indicating", "that", "the", "p65", ":", "p50", "heterodimer", "(", "NF-kappa", "B", ")", "is", "involved", "." ]

[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 9, 10, 0, 9, 10, 0, 0, 0, 0 ]

[ "Functional", "activation", "of", "gene", "expression", "by", "Pb", "was", "confirmed", "using", "primary", "CD4+", "T", "cells", "transfected", "with", "an", "NF-kappa", "B", "dependent", "reporter", "gene", "construct", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0 ]

[ "Pb", "did", "not", "activate", "NF-kappa", "B", "in", "4", "different", "T", "cell", "lines", ",", "suggesting", "that", "lymphoid", "cell", "lines", "may", "not", "be", "reliable", "surrogates", "for", "the", "study", "of", "transcriptional", "activation", "in", "human", "T", "cells", "." ]

[ 0, 0, 0, 0, 9, 10, 0, 0, 0, 5, 6, 6, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0 ]

[ "These", "data", "suggest", "that", "NF-kappa", "B", "may", "be", "an", "important", "molecular", "mediator", "of", "Pb-induced", "immunotoxicity", "." ]

[ 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Tyrosine", "kinase", "and", "cAMP-dependent", "protein", "kinase", "activities", "in", "CD40-activated", "human", "B", "lymphocytes", "." ]

[ 9, 10, 0, 9, 10, 10, 0, 0, 0, 7, 8, 8, 0 ]

[ "In", "vitro", ",", "human", "B", "lymphocytes", "undergo", "long-term", "proliferation", "when", "activated", "through", "CD40", ",", "a", "protein", "expressed", "on", "their", "cell", "surface", "." ]

[ 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "nature", "of", "CD40", "-dependent", "signals", "in", "proliferating", "fresh", "human", "Epstein-Barr", "virus-negative", "B", "lymphocytes", "is", "currently", "unknown", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0 ]

[ "In", "this", "study", ",", "a", "CD40", "-dependent", "B", "cell", "culture", "system", "was", "used", "to", "examine", "the", "role", "of", "different", "signal", "transduction", "elements", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Protein", "kinase", "C", "(", "PKC", ")", "depletion", "generated", "by", "a", "long-term", "phorbol", "12", "myristate", "13-acetate", "treatment", "had", "weak", "effects", "on", "proliferation", "." ]

[ 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Rather", ",", "tyrosine", "phosphorylation", "was", "shown", "to", "be", "directly", "involved", "in", "mediating", "CD40", "-dependent", "signals", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0 ]

[ "The", "use", "of", "the", "protein", "tyrosine", "kinase", "(", "PTK", ")", "-specific", "inhibitor", "herbimycin", "A", "dramatically", "decreased", "cellular", "proliferation", "without", "altering", "the", "activity", "of", "the", "human", "immunodeficiency", "virus-1", "long", "terminal", "repeat", "(", "HIV-1", "LTR", ")", ",", "a", "promoter", "largely", "dependent", "on", "the", "binding", "of", "nuclear", "factor", "kappa", "B", "(", "NF-", "kappa", "B", ")", "." ]

[ 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 10, 10, 0, 0 ]

[ "In", "contrast", ",", "the", "cAMP-dependent", "protein", "kinase", "specific", "inhibitor", "H-89", "totally", "inhibited", "HIV-1", "LTR", "activity", "at", "a", "concentration", "as", "low", "as", "100", "nM", "without", "affecting", "cellular", "proliferation", "." ]

[ 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Electrophoretic", "mobility", "shift", "assay", "(", "EMSA", ")", "and", "supershift", "assay", "using", "an", "NF-kappa", "B", "binding", "sequence", "from", "the", "kappa", "light", "chain", "as", "a", "probe", ",", "revealed", "that", "both", "p65", "(", "RelA", ")", "and", "c-Rel", "were", "present", "in", "CD40-stimulated", "B", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0, 0, 7, 8, 0 ]

[ "While", "PKC", "depletion", "did", "not", "alter", "the", "NF-kappa", "B", "level", ",", "treatment", "of", "B", "lymphocytes", "with", "H-89", "or", "herbimycin", "A", "provoked", "a", "decrease", "in", "the", "NF-kappa", "B", "level", "." ]

[ 0, 9, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]

[ "These", "observations", "establish", "the", "importance", "of", "different", "signal", "transducing", "pathways", "leading", "to", "CD40", "activation", "of", "B", "lymphocytes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 7, 8, 0 ]

[ "Interferons", "induce", "normal", "and", "aberrant", "retinoic-acid", "receptors", "type", "alpha", "in", "acute", "promyelocytic", "leukemia", "cells", ":", "potentiation", "of", "the", "induction", "of", "retinoid-dependent", "differentiation", "markers", "." ]

[ 9, 0, 0, 0, 0, 9, 10, 10, 10, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Treatment", "of", "the", "acute", "promyelocytic", "(", "APL", ")", "cell", "line", "NB4", "with", "interferon", "alpha", "(", "IFN", "(", "alpha", ")", ")", ",", "as", "well", "as", "IFN", "(", "beta", ")", "and", "gamma", ",", "results", "in", "an", "increased", "expression", "of", "the", "transcripts", "coding", "for", "retinoic-acid", "receptor", "type", "alpha", "(", "RAR", "(", "alpha", ")", ")", "and", "the", "leukemia-specific", "retinoic", "acid", "receptor", "PML-RAR", "." ]

[ 0, 0, 0, 5, 6, 6, 6, 6, 6, 6, 6, 0, 9, 10, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 10, 10, 10, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "Transcriptional", "induction", "of", "the", "RAR", "(", "alpha", ")", "and", "PML-RAR", "mRNAs", "is", "rapid", "and", "it", "is", "parallelled", "by", "an", "increase", "in", "the", "corresponding", "proteins", "." ]

[ 0, 0, 0, 0, 3, 4, 4, 4, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Up-regulation", "of", "RAR", "(", "alpha", ")", "and", "PML-RAR", "gene", "expression", "by", "IFN", "(", "alpha", ")", "is", "accompanied", "by", "a", "strong", "potentiation", "in", "the", "induction", "of", "2", "retinoid-dependent", "granulocytic", "markers", ",", "i.e.", ",", "granulocyte-colony-stimulating", "factor", "receptor", "mRNA", "and", "leukocyte", "alkaline", "phosphatase", "." ]

[ 0, 0, 1, 2, 2, 2, 0, 1, 2, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 4, 4, 0, 9, 10, 10, 0 ]

[ "However", ",", "IFN", "(", "alpha", ")", "does", "not", "have", "any", "effects", "on", "the", "retinoid-dependent", "regulation", "of", "the", "myeloid", "surface", "markers", "CD11b", "and", "CD33", "." ]

[ 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0 ]

[ "The", "IFN", "-dependent", "increase", "in", "RAR", "(", "alpha", ")", "levels", "and", "the", "enhancing", "effect", "of", "the", "cytokine", "on", "retinoid-dependent", "granulocytic", "markers", "expression", "may", "be", "a", "characteristic", "of", "PML-RAR", "positive", "cells", ",", "since", "the", "phenomena", "are", "not", "observed", "in", "HL-60", "promyelocytes", "." ]

[ 0, 9, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]

[ "Interferons", "as", "well", "as", "retinoids", "inhibit", "the", "growth", "of", "NB4", "cells", ",", "although", "the", "2", "classes", "of", "compounds", "do", "not", "significantly", "interact", "in", "terms", "of", "anti-proliferative", "activity", "." ]

[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "These", "results", "suggest", "the", "possible", "use", "of", "combinations", "between", "IFNs", "and", "retinoic", "acid", "in", "the", "cyto-differentiating", "treatment", "of", "APL", "patients", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Modulatory", "effects", "of", "glucocorticoids", "and", "catecholamines", "on", "human", "interleukin", "-12", "and", "interleukin", "-10", "production", ":", "clinical", "implications", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 10, 0, 0, 0, 0, 0 ]

[ "Interleukin-12", "(", "IL-12", ")", "is", "a", "key", "inducer", "of", "differentiation", "of", "uncommitted", "T", "helper", "(", "TH", ")", "cells", "toward", "the", "TH1", "phenotype", ",", "which", "regulates", "cellular", "immunity", ",", "whereas", "IL-10", "inhibits", "TH1", "functions", "and", "potentiates", "TH2-regulated", "responses", "(", "i.e.", ",", "humoral", "immunity", ")", "." ]

[ 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "To", "examine", "the", "potential", "effects", "of", "stress", "on", "TH1/TH2", "balance", ",", "we", "studied", "the", "ability", "of", "three", "prototype", "stress", "hormones-dexamethasone", "(", "a", "synthetic", "glucocorticoid", ")", "and", "the", "catecholamines", "norepinephrine", "and", "epinephrine-to", "alter", "the", "production", "of", "IL-12", "(", "p70", ")", "and", "IL-10", "induced", "by", "bacterial", "lipopolysaccharide", "(", "LPS", ")", "in", "human", "whole", "blood", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Dexamethasone", "inhibited", "LPS-induced", "bioactive", "IL-12", "production", "in", "a", "dose-dependent", "fashion", "and", "at", "physiologically", "relevant", "concentrations", ";", "it", "had", "no", "effect", "on", "IL-10", "secretion", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "The", "glucocorticoid-induced", "reduction", "of", "IL-12", "production", "was", "antagonized", "by", "RU", "486", ",", "a", "glucocorticoid-receptor", "antagonist", ",", "suggesting", "that", "it", "was", "mediated", "by", "the", "glucocorticoid", "receptor", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "Norepinephrine", "and", "epinephrine", "also", "suppressed", "IL-12", "production", "in", "a", "dose-dependent", "fashion", "and", "at", "physiological", "concentrations", ";", "both", "catecholamines", ",", "however", ",", "dose-dependently", "increased", "the", "production", "of", "IL-10", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "The", "effects", "of", "either", "catecholamine", "on", "IL-12", "or", "IL-10", "secretion", "were", "blocked", "completely", "by", "propranolol", ",", "a", "beta-adrenoreceptor", "antagonist", ",", "indicating", "that", "they", "were", "mediated", "by", "the", "beta-adrenergic", "receptor", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "These", "findings", "suggest", "that", "the", "central", "nervous", "system", "may", "regulate", "IL-12", "and", "IL-10", "secretion", "and", ",", "hence", ",", "TH1/TH2", "balance", "via", "the", "peripheral", "end-effectors", "of", "the", "stress", "system", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Thus", ",", "stress", "may", "cause", "a", "selective", "suppression", "of", "TH1", "functions", "and", "a", "shift", "toward", "a", "TH2", "cytokine", "pattern", "rather", "than", "generalized", "TH", "suppression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "TH1-to-TH2", "shift", "may", "be", "responsible", "for", "the", "stress-induced", "susceptibility", "of", "the", "organism", "to", "certain", "infections", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Through", "the", "same", "or", "a", "reciprocal", "mechanism", ",", "states", "associated", "with", "chronic", "hyperactivity", "or", "hypoactivity", "of", "the", "stress", "system", "might", "influence", "the", "susceptibility", "of", "an", "individual", "to", "certain", "autoimmune", ",", "allergic", ",", "infectious", ",", "or", "neoplastic", "diseases", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Activation", "of", "human", "monocytic", "cells", "by", "Treponema", "pallidum", "and", "Borrelia", "burgdorferi", "lipoproteins", "and", "synthetic", "lipopeptides", "proceeds", "via", "a", "pathway", "distinct", "from", "that", "of", "lipopolysaccharide", "but", "involves", "the", "transcriptional", "activator", "NF-kappa", "B", "." ]

[ 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "There", "is", "increasing", "evidence", "that", "lipoproteins", "of", "Treponema", "pallidum", "and", "Borrelia", "burgdorferi", "are", "key", "inflammatory", "mediators", "during", "syphilis", "and", "Lyme", "disease", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "A", "principal", "objective", "of", "the", "present", "study", "was", "to", "identify", "more", "precisely", "similarities", "and", "divergences", "among", "lipopolysaccharide", "(", "LPS", ")", "-", "and", "lipoprotein-lipopeptide-induced", "immune", "cell", "signaling", "events", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Like", "LPS", ",", "purified", "native", "B.", "burgdorferi", "OspA", "and", "synthetic", "analogs", "of", "OspA", ",", "OspB", ",", "and", "two", "T.", "pallidum", "lipoproteins", "(", "Tpp47", "and", "Tpp17", ")", "all", "induced", "NF-kappa", "B", "translocation", "in", "THP-1", "human", "monocytoid", "cells", "." ]

[ 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 10, 0, 0, 5, 6, 6, 6, 0 ]

[ "Acylation", "of", "OspA", "and", "the", "synthetic", "peptides", "was", "requisite", "for", "cell", "activation", "." ]

[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Polymyxin", "B", "abrogated", "only", "the", "response", "to", "LPS", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "By", "using", "70Z/3-derived", "pre-B-cell", "lines", "either", "lacking", "or", "expressing", "human", "CD14", "(", "the", "LPS", "receptor", ")", ",", "it", "was", "observed", "that", "expression", "of", "human", "CD14", "imparted", "responsiveness", "to", "LPS", "but", "not", "to", "OspA", "or", "spirochetal", "lipopeptides", "(", "assessed", "by", "induction", "of", "NF-kappa", "B", "and", "expression", "of", "surface", "immunoglobulin", "M", ")", "." ]

[ 0, 0, 0, 5, 6, 0, 0, 0, 0, 9, 10, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0, 0 ]

[ "Finally", ",", "the", "biological", "relevance", "of", "the", "observation", "that", "T.", "pallidum", "lipoproteins", "-lipopeptides", "induce", "both", "NF-kappa", "B", "and", "cytokine", "production", "in", "monocytes", "was", "supported", "by", "the", "ability", "of", "the", "synthetic", "analogs", "to", "promote", "human", "immunodeficiency", "virus", "replication", "in", "chronically", "infected", "U1", "monocytoid", "cells", ";", "these", "observations", "also", "suggest", "a", "potential", "mechanism", "whereby", "a", "syphilitic", "chancre", "can", "serve", "as", "a", "cofactor", "for", "human", "immunodeficiency", "virus", "transmission", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "combined", "data", "lend", "additional", "support", "to", "the", "proposal", "that", "spirochetal", "lipoproteins", "and", "LPS", "initiate", "monocyte", "activation", "via", "different", "cell", "surface", "events", "but", "that", "the", "signaling", "pathways", "ultimately", "converge", "to", "produce", "qualitatively", "similar", "cellular", "responses", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "LYSP100", "-associated", "nuclear", "domains", "(", "LANDs", ")", ":", "description", "of", "a", "new", "class", "of", "subnuclear", "structures", "and", "their", "relationship", "to", "PML", "nuclear", "bodies", "." ]

[ 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0 ]

[ "The", "PML", "gene", "is", "fused", "to", "the", "retinoic", "acid", "receptor", "alpha", "(", "RAR", "alpha", ")", "gene", "in", "t", "(", "15", ";", "17", ")", "acute", "promyelocytic", "leukemia", "(", "APL", ")", ",", "creating", "a", "PML-RAR", "alpha", "fusion", "oncoprotein", "." ]

[ 0, 1, 2, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "The", "PML", "gene", "product", "has", "been", "localized", "to", "subnuclear", "dot-like", "structures", "variously", "termed", "PODs", ",", "ND10s", ",", "Kr", "bodies", ",", "or", "PML", "nuclear", "bodies", "(", "PML", "NBs", ")", "." ]

[ 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 0, 0 ]

[ "The", "present", "study", "describes", "the", "cloning", "of", "a", "lymphoid-restricted", "gene", ",", "LYSP100", ",", "that", "is", "homologous", "to", "another", "protein", "that", "localizes", "to", "PML", "NBs", ",", "SP100", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0 ]

[ "In", "addition", "to", "SP100", "homology", "regions", ",", "one", "LYSP100", "cDNA", "isoform", "contains", "a", "bromodomain", "and", "a", "PHD/TTC", "domain", ",", "which", "are", "present", "in", "a", "variety", "of", "transcriptional", "regulatory", "proteins", "." ]

[ 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "By", "immunofluorescence", ",", "LYSP100", "was", "localized", "to", "nuclear", "dots", "that", "were", "surprisingly", "largely", "nonoverlapping", "with", "PML", "NBs", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "However", ",", "a", "minority", "of", "LYSP100", "nuclear", "dots", "exactly", "colocalized", "with", "PML", "and", "SP100", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 9, 0 ]