id
stringlengths
1
5
tokens
sequence
ner_tags
sequence
4101
[ "To", "address", "possible", "mechanisms", "that", "could", "explain", "this", "selective", "activity", "of", "c-ErbB", ",", "we", "analyzed", "the", "ability", "of", "these", "receptors", "to", "activate", "the", "different", "members", "of", "the", "Stat", "transcription", "factor", "family", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]
4102
[ "Ligand", "activation", "of", "c-ErbB", "induced", "the", "tyrosine", "phosphorylation", ",", "DNA-binding", ",", "and", "reporter", "gene", "transcription", "of", "Stat", "5b", "in", "erythroblasts", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 7, 0 ]
4103
[ "In", "contrast", ",", "ligand", "activation", "of", "c-Kit", "was", "unable", "to", "induce", "any", "of", "these", "effects", "in", "the", "same", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4104
[ "Activation", "of", "the", "erythropoietin", "receptor", "caused", "specific", "DNA-binding", "of", "Stat", "5b", ",", "but", "failed", "to", "induce", "reporter", "gene", "transcription", "." ]
[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4105
[ "These", "biochemical", "findings", "correlate", "perfectly", "with", "the", "selective", "ability", "of", "c-ErbB", "to", "cause", "sustained", "self", "renewal", "in", "erythroid", "progenitors", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
4106
[ "Defective", "transcription", "of", "the", "IL-2", "gene", "is", "associated", "with", "impaired", "expression", "of", "c-Fos", ",", "FosB", ",", "and", "JunB", "in", "anergic", "T", "helper", "1", "cells", "." ]
[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0, 0, 7, 8, 8, 8, 0 ]
4107
[ "Anergic", "CD4+", "Th", "cells", "do", "not", "produce", "IL-2", "when", "challenged", "with", "Ag-pulsed", "accessory", "cells", "because", "of", "a", "transcriptional", "defect", "." ]
[ 0, 0, 7, 8, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4108
[ "In", "this", "work", ",", "we", "report", "that", "these", "anergic", "T", "cells", "are", "defective", "in", "their", "ability", "to", "up-regulate", "protein", "binding", "and", "transactivation", "at", "two", "critical", "IL-2", "DNA", "enhancer", "elements", ":", "NF-AT", "(", "nuclear", "factor", "of", "activated", "T", "cells", ";", "a", "sequence", "that", "binds", "a", "heterotrimeric", "NFATp", ",", "Fos", ",", "and", "Jun", "protein", "complex", ")", "and", "Activator", "Protein-1", "(", "AP-1", ")", "(", "that", "binds", "Fos", "and", "Jun", "heterodimers", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 9, 0, 9, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 10, 10, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 9, 0, 9, 10, 0, 0 ]
4109
[ "Western", "blot", "analysis", "of", "nuclear", "extracts", "showed", "that", "the", "impaired", "DNA-protein", "interactions", "in", "anergic", "T", "cells", "were", "associated", "with", "poor", "expression", "of", "the", "inducible", "AP-1", "family", "members", "c-Fos", ",", "FosB", ",", "and", "JunB", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 9, 0, 9, 0, 0, 9, 0 ]
4110
[ "However", ",", "the", "reduced", "expression", "of", "these", "proteins", "was", "not", "the", "result", "of", "a", "global", "TCR/CD3", "-signaling", "defect", "because", "CD3", "cross-linking", "induced", "an", "equivalent", "increase", "in", "intracellular-free", "calcium", "ions", ",", "as", "well", "as", "NFATp", "dephosphorylation", ",", "translocation", "to", "the", "nucleus", ",", "and", "DNA", "binding", "in", "both", "normal", "and", "anergic", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
4111
[ "Thus", ",", "defective", "IL-2", "gene", "transcription", "appears", "to", "be", "due", ",", "at", "least", "in", "part", ",", "to", "a", "selective", "block", "in", "the", "expression", "of", "the", "AP-1", "Fos", "and", "Jun", "family", "members", "in", "anergic", "T", "cells", "." ]
[ 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 10, 0, 0, 7, 8, 0 ]
4112
[ "Sequence-specific", "DNA", "binding", "of", "the", "B-cell-specific", "coactivator", "OCA-B", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 10, 0 ]
4113
[ "B-cell-specific", "transcription", "of", "immunoglobulin", "genes", "is", "mediated", "by", "the", "interaction", "of", "a", "POU", "domain", "containing", "transcription", "factor", "Oct-1", "or", "Oct-2", ",", "with", "the", "B-cell-specific", "coactivator", "OCA-B", "(", "Bob-1", ",", "OBF-1", ")", "and", "a", "prototype", "octamer", "element", "." ]
[ 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 9, 10, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]
4114
[ "We", "find", "that", "OCA-B", "binds", "DNA", "directly", "in", "the", "major", "groove", "between", "the", "two", "subdomains", "of", "the", "POU", "domain", ",", "requiring", "both", "an", "A", "at", "the", "fifth", "position", "of", "the", "octamer", "element", "and", "contact", "with", "the", "POU", "domain", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4115
[ "An", "amino-terminal", "fragment", "of", "OCA-B", "binds", "the", "octamer", "site", "in", "the", "absence", "of", "a", "POU", "domain", "with", "the", "same", "sequence", "specificity", "." ]
[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4116
[ "Coactivator", "OCA-B", "may", "undergo", "a", "POU-dependent", "conformational", "change", "that", "exposes", "its", "amino", "terminus", ",", "allowing", "it", "to", "recognize", "specific", "DNA", "sequences", "in", "the", "major", "groove", "within", "the", "binding", "site", "for", "Oct-1", "or", "Oct-2", "." ]
[ 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0 ]
4117
[ "The", "recognition", "of", "both", "the", "POU", "domain", "and", "the", "octamer", "sequence", "by", "OCA-B", "provides", "a", "mechanism", "for", "differential", "regulation", "of", "octamer", "sites", "containing", "genes", "by", "the", "ubiquitous", "factor", "Oct-1", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
4118
[ "Uneven", "X", "inactivation", "in", "a", "female", "monozygotic", "twin", "pair", "with", "Fabry", "disease", "and", "discordant", "expression", "of", "a", "novel", "mutation", "in", "the", "alpha-galactosidase", "A", "gene", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0 ]
4119
[ "We", "describe", "two", "female", "monozygotic", "(", "MZ", ")", "twins", "heterozygous", "for", "Fabry", "disease", ",", "an", "X", "linked", "disorder", "resulting", "from", "the", "deficient", "activity", "of", "alpha-galactosidase", "A", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]
4120
[ "While", "one", "of", "the", "twins", "was", "clinically", "affected", ",", "the", "other", "was", "asymptomatic", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4121
[ "Enzymatic", "assay", "of", "alpha-galactosidase", "in", "blood", "leucocytes", ",", "skin", "fibroblasts", ",", "Epstein-Barr", "virus", "transformed", "lymphoid", "cell", "lines", ",", "and", "hair", "follicles", "of", "the", "twins", "and", "their", "parents", "confirmed", "the", "heterozygous", "status", "of", "the", "twins", "and", "indicated", "that", "Fabry", "disease", "had", "occurred", "as", "a", "result", "of", "a", "de", "novo", "mutation", "." ]
[ 0, 0, 0, 9, 0, 7, 8, 0, 7, 8, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4122
[ "The", "son", "of", "the", "unaffected", "twin", "sister", "was", "shown", "to", "be", "hemizygous", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4123
[ "Molecular", "analysis", "of", "the", "alpha-galactosidase", "A", "gene", "permitted", "the", "identification", "of", "an", "as", "yet", "undescribed", "point", "mutation", "at", "position", "10182", "of", "exon", "5", "which", "causes", "an", "Asp", "to", "Asn", "substitution", "at", "codon", "231", "." ]
[ 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4124
[ "Single", "strand", "conformation", "polymorphism", "(", "SSCP", ")", "analysis", "again", "showed", "the", "heterozygous", "status", "of", "the", "twins", "and", "a", "normal", "pattern", "in", "their", "parents", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4125
[ "The", "basis", "for", "the", "discordant", "expression", "of", "this", "d", "novo", "mutation", "in", "the", "twins", "was", "investigated", "by", "studying", "their", "X", "inactivation", "status", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4126
[ "Analysis", "of", "the", "inactive", "X", "specific", "methylation", "at", "the", "androgen", "receptor", "gene", "showed", "unbalanced", "inactivation", "in", "the", "twins", "'", "fibroblasts", "and", "in", "opposite", "directions", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4127
[ "While", "the", "maternally", "derived", "X", "chromosome", "was", "preferentially", "active", "in", "the", "asymptomatic", "twin", ",", "the", "paternal", "X", "chromosome", "was", "active", "in", "the", "other", ",", "affected", "twin", "and", "was", "found", "in", "her", "hemizygotic", "nephew", "." ]
[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4128
[ "These", "data", "suggest", "that", "the", "paternal", "X", "chromosome", "carries", "the", "de", "novo", "alpha-galactosidase", "A", "mutation", "and", "that", "uneven", "X", "inactivation", "is", "the", "underlying", "mechanism", "for", "disease", "expression", "in", "this", "novel", "female", "MZ", "twin", "pair", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4129
[ "This", "is", "the", "first", "documented", "case", "of", "female", "twins", "discordant", "for", "Fabry", "disease", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4130
[ "Precise", "alignment", "of", "sites", "required", "for", "mu", "enhancer", "activation", "in", "B", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 7, 8, 0 ]
4131
[ "The", "lymphocyte-specific", "immunoglobulin", "mu", "heavy-chain", "gene", "intronic", "enhancer", "is", "regulated", "by", "multiple", "nuclear", "factors", "." ]
[ 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0 ]
4132
[ "The", "previously", "defined", "minimal", "enhancer", "containing", "the", "muA", ",", "muE3", ",", "and", "muB", "sites", "is", "transactivated", "by", "a", "combination", "of", "the", "ETS-domain", "proteins", "PU.1", "and", "Ets-1", "in", "nonlymphoid", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 7, 8, 0 ]
4133
[ "The", "core", "GGAAs", "of", "the", "muA", "and", "muB", "sites", "are", "separated", "by", "30", "nucleotides", ",", "suggesting", "that", "ETS", "proteins", "bind", "to", "these", "sites", "from", "these", "same", "side", "of", "the", "DNA", "helix", "." ]
[ 0, 0, 0, 0, 0, 1, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4134
[ "We", "tested", "the", "necessity", "for", "appropriate", "spatial", "alignment", "of", "these", "elements", "by", "using", "mutated", "enhancers", "with", "altered", "spacings", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4135
[ "A", "4-", "or", "10-bp", "insertion", "between", "muE3", "and", "muB", "inactivated", "the", "mu", "enhancer", "in", "S194", "plasma", "cells", "but", "did", "not", "affect", "in", "vitro", "binding", "of", "Ets-1", ",", "PU.1", ",", "or", "the", "muE3-binding", "protein", "TFE3", ",", "alone", "or", "in", "pairwise", "combinations", "." ]
[ 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 1, 2, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0 ]
4136
[ "Circular", "permutation", "and", "phasing", "analyses", "demonstrated", "that", "PU.1", "binding", "but", "not", "TFE3", "or", "Ets-1", "bends", "mu", "enhancer", "DNA", "toward", "the", "major", "groove", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 9, 0, 1, 2, 2, 0, 0, 0, 0, 0 ]
4137
[ "We", "propose", "that", "the", "requirement", "for", "precise", "spacing", "of", "the", "muA", "and", "muB", "elements", "is", "due", "in", "part", "to", "a", "directed", "DNA", "bend", "induced", "by", "PU.1", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
4138
[ "[", "Cortisone-resistant", "bronchial", "asthma", "]" ]
[ 0, 0, 0, 0, 0 ]
4139
[ "There", "is", "general", "agreement", "on", "the", "inflammatory", "pathogenesis", "of", "bronchial", "asthma", ":", "an", "accumulation", "of", "activated", "eosinophils", ",", "degranulated", "mast", "cells", ",", "T", "lymphocytes", "and", "in", "very", "severe", "forms", ",", "granulocytes", "has", "constantly", "been", "found", "in", "the", "bronchial", "mucosa", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 7, 8, 0, 7, 8, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4140
[ "In", "allergic", "bronchial", "asthma", ",", "inflammation", "seems", "to", "be", "orchestrated", "predominantly", "by", "a", "subset", "of", "T", "lymphocytes", ",", "with", "a", "phenotype", "similar", "to", "the", "Th2", "subset", "able", "to", "produce", "IL-4", "and", "IL-5", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 9, 0, 9, 0 ]
4141
[ "Although", "corticosteroids", "are", "the", "most", "potent", "therapeutic", "agents", "used", "for", "this", "disease", ",", "their", "anti-inflammatory", "effect", "differs", "from", "patient", "to", "patient", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4142
[ "Some", "criteria", "which", "can", "be", "used", "to", "define", "steroid-resistant", "bronchial", "asthma", "are", "listed", "here", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4143
[ "This", "review", "analyzes", "various", "molecular", "alterations", "responsible", "for", "the", "deficient", "response", "to", "corticosteroid", "treatment", "observed", "in", "steroid-resistant", "bronchial", "asthmatic", "subjects", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4144
[ "New", "knowledge", "on", "the", "mechanism", "of", "steroid", "resistance", "may", "have", "important", "implications", "for", "the", "treatment", "of", "chronic", "asthma", "and", "other", "diseases", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4145
[ "Mechanisms", "of", "transactivation", "by", "nuclear", "factor", "of", "activated", "T", "cells-1", "." ]
[ 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0 ]
4146
[ "Nuclear", "factor", "of", "activated", "T", "cells-family", "proteins", "(", "NFAT1/NFATp", ",", "NFATc", ",", "NFAT3", ",", "and", "NFAT4/NFATx/NFATc3", ")", "play", "a", "key", "role", "in", "the", "transcription", "of", "cytokine", "genes", "and", "other", "genes", "during", "the", "immune", "response", "." ]
[ 9, 10, 10, 10, 10, 10, 10, 0, 9, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4147
[ "We", "have", "defined", "the", "mechanisms", "of", "transactivation", "by", "NFAT1", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
4148
[ "NFAT1", "possesses", "two", "transactivation", "domains", "whose", "sequences", "are", "not", "conserved", "in", "the", "other", "NFAT-family", "proteins", ",", "and", "a", "conserved", "DNA-binding", "domain", "that", "mediates", "the", "recruitment", "of", "cooperating", "nuclear", "transcription", "factors", "even", "when", "it", "is", "expressed", "in", "the", "absence", "of", "other", "regions", "of", "the", "protein", "." ]
[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4149
[ "The", "activity", "of", "the", "NH2-terminal", "transactivation", "domain", "is", "modulated", "by", "an", "adjacent", "regulatory", "region", "that", "contains", "several", "conserved", "sequence", "motifs", "represented", "only", "in", "the", "NFAT", "family", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]
4150
[ "Our", "results", "emphasize", "the", "multiple", "levels", "at", "which", "NFAT-dependent", "transactivation", "is", "regulated", ",", "and", "predict", "significant", "differences", "in", "the", "architecture", "of", "cooperative", "transcription", "complexes", "containing", "different", "NFAT-family", "proteins", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]
4151
[ "Induction", "of", "activator", "protein", "(", "AP", ")", "-1", "and", "nuclear", "factor-kappaB", "by", "CD28", "stimulation", "involves", "both", "phosphatidylinositol", "3-kinase", "and", "acidic", "sphingomyelinase", "signals", "." ]
[ 0, 0, 9, 10, 10, 10, 10, 10, 0, 9, 10, 0, 9, 0, 0, 0, 9, 10, 0, 9, 10, 0, 0 ]
4152
[ "A", "major", "obstacle", "in", "understanding", "the", "signaling", "events", "that", "follow", "CD28", "receptor", "ligation", "arises", "from", "the", "fact", "that", "CD28", "acts", "as", "a", "costimulus", "to", "TCR", "engagement", ",", "making", "it", "difficult", "to", "assess", "the", "relative", "contribution", "of", "CD28", "signals", "as", "distinct", "from", "those", "of", "the", "TCR", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
4153
[ "To", "overcome", "this", "problem", ",", "we", "have", "exploited", "the", "observation", "that", "activated", "human", "T", "cell", "blasts", "can", "be", "stimulated", "via", "the", "CD28", "surface", "molecule", "in", "the", "absence", "of", "antigenic", "challenge", ";", "thus", ",", "we", "have", "been", "able", "to", "observe", "the", "response", "of", "normal", "T", "cells", "to", "CD28", "activation", "in", "isolation", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 9, 0, 0, 0, 0 ]
4154
[ "Using", "this", "system", ",", "we", "observed", "that", "CD28", "stimulation", "by", "B7-transfected", "CHO", "cells", "induced", "a", "proliferative", "response", "in", "T", "cells", "that", "was", "not", "accompanied", "by", "measurable", "IL-2", "production", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]
4155
[ "However", ",", "subsequent", "analysis", "of", "transcription", "factor", "generation", "revealed", "that", "B7", "stimulation", "induced", "both", "activator", "protein-1", "(", "AP-1", ")", "and", "nuclear", "factor-kappaB", "(", "NF-kappaB", ")", "complexes", ",", "but", "not", "NF-AT", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 10, 0, 9, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 9, 0 ]
4156
[ "In", "contrast", ",", "engagement", "of", "the", "TCR", "by", "class", "II", "MHC/superantigen", ",", "either", "with", "or", "without", "CD28", "ligation", ",", "resulted", "in", "the", "induction", "of", "NF-AT", ",", "AP-1", ",", "and", "NF-kappaB", "as", "well", "as", "IL-2", "production", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0, 9, 0, 0 ]
4157
[ "Using", "selective", "inhibitors", ",", "we", "investigated", "the", "signaling", "pathways", "involved", "in", "the", "CD28", "-mediated", "induction", "of", "AP-1", "and", "NF-kappaB", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 9, 0 ]
4158
[ "This", "revealed", "that", "NF-kappaB", "generation", "was", "sensitive", "to", "chloroquine", ",", "an", "inhibitor", "of", "acidic", "sphingomyelinase", ",", "but", "not", "to", "the", "phosphatidylinositol", "3-kinase", "inhibitor", ",", "wortmannin", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0 ]
4159
[ "In", "contrast", ",", "AP-1", "generation", "was", "inhibited", "by", "wortmannin", "and", "was", "also", "variably", "sensitive", "to", "chloroquine", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4160
[ "These", "data", "suggest", "that", "in", "activated", "normal", "T", "cells", ",", "CD28", "-derived", "signals", "can", "stimulate", "proliferation", "at", "least", "in", "part", "via", "NF-kappaB", "and", "AP-1", "generation", ",", "and", "that", "this", "response", "uses", "both", "acidic", "sphingomyelinase", "and", "phosphatidylinositol", "3-kinase", "-linked", "pathways", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 0, 0, 0 ]
4161
[ "Presence", "of", "a", "variant", "form", "of", "the", "estrogen", "receptor", "in", "peripheral", "blood", "mononuclear", "cells", "from", "normal", "individuals", "and", "lupus", "patients", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0 ]
4162
[ "Estrogen", "may", "participate", "in", "the", "pathogenesis", "of", "systemic", "lupus", "erythematosus", "(", "SLE", ")", "via", "its", "intracellular", "receptor", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4163
[ "To", "investigate", "the", "presence", "of", "various", "isoforms", "of", "the", "estrogen", "receptor", "(", "ER", ")", "in", "SLE", "we", "isolated", "RNA", "from", "mononuclear", "cells", "of", "lupus", "patients", "and", "normal", "controls", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0 ]
4164
[ "Using", "RT-PCR", "we", "were", "able", "to", "identify", "both", "the", "full", "length", "wild-type", "form", "and", "an", "isoform", "of", "the", "ER", "which", "precisely", "lacks", "exon", "V", "in", "both", "patient", "and", "normal", "individuals", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4165
[ "Our", "results", ",", "although", "limited", ",", "suggest", "that", "normal", "individuals", "can", "express", "both", "the", "wild-type", "and", "truncated", "version", "at", "the", "same", "time", ",", "whereas", "lupus", "patients", "only", "express", "either", "the", "wild-type", "or", "the", "truncated", "ER", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
4166
[ "This", "finding", "may", "lead", "to", "a", "better", "understanding", "of", "the", "reasons", "for", "the", "prevalence", "of", "lupus", "in", "females", "and", "of", "the", "estrogenic", "effects", "on", "SLE", "disease", "activity", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4167
[ "Regulation", "of", "sialoadhesin", "expression", "on", "rat", "macrophages", "." ]
[ 0, 0, 9, 0, 0, 7, 8, 0 ]
4168
[ "Induction", "by", "glucocorticoids", "and", "enhancement", "by", "IFN-beta", ",", "IFN-gamma", ",", "IL-4", ",", "and", "lipopolysaccharide", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 9, 0, 0, 0, 0 ]
4169
[ "Sialoadhesin", "is", "a", "macrophage-restricted", "member", "of", "the", "Ig", "superfamily", "that", "mediates", "adhesion", "with", "lymphoid", "and", "myeloid", "cells", "." ]
[ 9, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
4170
[ "It", "is", "expressed", "on", "a", "subpopulation", "of", "macrophages", "in", "lymphoid", "tissues", "and", "in", "chronic", "inflammation", "(", "e.g.", ",", "during", "autoimmune", "diseases", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4171
[ "We", "have", "studied", "the", "regulation", "of", "sialoadhesin", "expression", "in", "vitro", "and", "show", "that", "glucocorticoids", "(", "GC", ")", "induce", "sialoadhesin", "expression", "on", "freshly", "isolated", "rat", "macrophages", "and", "the", "rat", "macrophage", "cell", "line", "R2", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 7, 8, 0, 0, 5, 6, 6, 6, 6, 0 ]
4172
[ "The", "cytokines", "IFN-beta", ",", "IFN-gamma", ",", "IL-4", ",", "and", "LPS", ",", "although", "unable", "to", "induce", "sialoadhesin", "expression", "by", "themselves", ",", "were", "able", "to", "enhance", "GC-mediated", "induction", "of", "sialoadhesin", "." ]
[ 0, 9, 10, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
4173
[ "Sialoadhesin", "expression", "was", "functional", "as", "shown", "by", "cell", "adhesion", "assays", "with", "human", "RBCs", "." ]
[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
4174
[ "Northern", "blotting", "experiments", "indicated", "that", "regulation", "predominantly", "occurred", "at", "the", "mRNA", "level", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4175
[ "Comparison", "of", "the", "different", "combinations", "of", "GC", "and", "cytokines", "/LPS", "revealed", "differences", "in", "the", "level", "of", "GC-dependent", "enhancement", "of", "sialoadhesin", "expression", ",", "with", "IFN-beta", "and", "IL-4", "being", "more", "potent", "than", "IFN-gamma", "and", "LPS", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 9, 0, 0, 0 ]
4176
[ "Moreover", ",", "the", "effects", "of", "IFN-gamma", "and", "LPS", "could", "be", "reproduced", "by", "priming", ",", "whereas", "IFN-beta", "and", "IL-4", "were", "required", "simultaneously", "with", "GC", "." ]
[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0 ]
4177
[ "The", "regulation", "of", "sialoadhesin", "expression", "was", "mediated", "by", "the", "GC", "receptor", ",", "and", "not", "by", "mineralocorticoid", "receptor", ",", "as", "shown", "by", "inhibition", "experiments", "with", "specific", "antagonists", "." ]
[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4178
[ "Finally", ",", "it", "is", "demonstrated", "that", "macrophages", "in", "the", "adrenal", "gland", ",", "the", "major", "site", "of", "endogenous", "GC", "production", ",", "express", "sialoadhesin", "." ]
[ 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
4179
[ "This", "study", "demonstrates", "that", "GC", "act", "as", "a", "primary", "inducer", "of", "sialoadhesin", "expression", "on", "rat", "macrophages", ",", "and", "that", "the", "response", "can", "be", "enhanced", "by", "IFN-beta", ",", "T", "cell-derived", "cytokines", ",", "or", "LPS", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]
4180
[ "Apoptosis", "mediated", "by", "HIV", "protease", "is", "preceded", "by", "cleavage", "of", "Bcl-2", "." ]
[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0 ]
4181
[ "Expression", "of", "the", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV", ")", "protease", "in", "cultured", "cells", "leads", "to", "apoptosis", ",", "preceded", "by", "cleavage", "of", "bcl-2", ",", "a", "key", "negative", "regulator", "of", "cell", "death", "." ]
[ 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4182
[ "In", "contrast", ",", "a", "high", "level", "of", "bcl-2", "protects", "cells", "in", "vitro", "and", "in", "vivo", "from", "the", "viral", "protease", "and", "prevents", "cell", "death", "following", "HIV", "infection", "of", "human", "lymphocytes", ",", "while", "reducing", "the", "yields", "of", "viral", "structural", "proteins", ",", "infectivity", ",", "and", "tumor", "necrosis", "factor", "alpha", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]
4183
[ "We", "present", "a", "model", "for", "HIV", "replication", "in", "which", "the", "viral", "protease", "depletes", "the", "infected", "cells", "of", "bcl-2", ",", "leading", "to", "oxidative", "stress-dependent", "activation", "of", "NF", "kappa", "B", ",", "a", "cellular", "factor", "required", "for", "HIV", "transcription", ",", "and", "ultimately", "to", "cell", "death", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4184
[ "Purified", "bcl-2", "is", "cleaved", "by", "HIV", "protease", "between", "phenylalanine", "112", "and", "alanine", "113", "." ]
[ 0, 9, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0 ]
4185
[ "The", "results", "suggest", "a", "new", "option", "for", "HIV", "gene", "therapy", ";", "bcl-2", "muteins", "that", "have", "noncleavable", "alterations", "surrounding", "the", "HIV", "protease", "cleavage", "site", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0 ]
4186
[ "Alpha", "4", "beta", "1", "(", "CD49d/CD29", ")", "integrin", "costimulation", "of", "human", "T", "cells", "enhances", "transcription", "factor", "and", "cytokine", "induction", "in", "the", "absence", "of", "altered", "sensitivity", "to", "anti-CD3", "stimulation", "." ]
[ 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]
4187
[ "The", "integrin", "alpha", "4", "beta", "1", "can", "provide", "a", "costimulus", "to", "induce", "IL-2", "secretion", "and", "IL-2R", "expression", "leading", "to", "enhanced", "proliferation", "of", "purified", ",", "peripheral", "blood", "T", "cells", "." ]
[ 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0 ]
4188
[ "Similar", "to", "expression", "of", "IL-2", ",", "we", "demonstrated", "that", "recombinant", "vascular-cell", "adhesion", "molecule-1", ",", "when", "co-immobilized", "with", "anti-CD3", "mAb", ",", "significantly", "enhanced", "the", "induction", "of", "transcription", "factors", "NF-AT", ",", "AP-1", ",", "and", "NF-kappa", "B", "as", "determined", "by", "electromobility", "shift", "assays", "." ]
[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0 ]
4189
[ "alpha", "4", "beta", "1", "ligation", "alone", "had", "no", "effect", "on", "transcription", "factor", "binding", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4190
[ "The", "requirements", "for", "induction", "of", "transcription", "factors", "reflected", "the", "requirements", "for", "the", "secretion", "of", "multiple", "cytokines", ",", "including", "IL-2", ",", "TNF-alpha", ",", "IFN-gamma", ",", "and", "granulocyte", "macrophage-CSF", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 9, 0, 0, 9, 10, 0 ]
4191
[ "In", "contrast", "to", "freshly", "isolated", "T", "cells", ",", "in", "vitro-cultured", "T", "cells", "did", "not", "require", "costimulation", "for", "cytokine", "secretion", "in", "response", "to", "anti-CD3", "alone", "." ]
[ 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]
4192
[ "Comparison", "of", "the", "dose", "response", "to", "anti-CD3", "stimulation", "demonstrated", "that", "half-maximal", "induction", "of", "IL-2", "was", "achieved", "using", "the", "same", "dose", "of", "anti-CD3", "for", "both", "freshly", "isolated", "and", "cultured", "T", "cells", "." ]
[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]
4193
[ "Furthermore", ",", "the", "dose", "of", "OKT3", "required", "to", "achieve", "half-maximal", "activation", "was", "the", "same", "using", "PMA", "or", "different", "concentrations", "of", "alpha", "4", "beta", "1", "ligands", "." ]
[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4194
[ "Therefore", ",", "costimulation", "by", "alpha", "4", "beta", "1", "ligands", "was", "not", "due", "to", "stabilization", "of", "the", "interaction", "of", "the", "cells", "with", "its", "substrate", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4195
[ "We", "conclude", ",", "rather", ",", "that", "alpha", "4", "beta", "1", "in", "freshly", "isolated", "T", "cells", "delivers", "a", "distinct", "signal", "that", "synergizes", "early", "with", "signals", "initiated", "by", "TCR/CD3", "ligation", "to", "induce", "DNA", "binding", "of", "multiple", "transcription", "factors", "required", "for", "cytokine", "gene", "induction", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4196
[ "Inhibition", "of", "T", "lymphocyte", "activation", "by", "cAMP", "is", "associated", "with", "down-regulation", "of", "two", "parallel", "mitogen-activated", "protein", "kinase", "pathways", ",", "the", "extracellular", "signal-related", "kinase", "and", "c-Jun", "N-terminal", "kinase", "." ]
[ 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 9, 10, 10, 0, 9, 10, 10, 0 ]
4197
[ "The", "induction", "of", "T", "cell", "proliferation", "requires", "signals", "from", "the", "TCR", "and", "a", "co-receptor", "molecule", ",", "such", "as", "CD28", ",", "that", "activate", "parallel", "and", "partially", "cross-reactive", "signaling", "pathways", "." ]
[ 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]
4198
[ "These", "pathways", "are", "disrupted", "by", "agonists", "that", "utilize", "adenylate", "cyclase", "and", "cAMP-dependent", "protein", "kinase", "A", "(", "PKA", ")", "." ]
[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 10, 10, 0, 9, 0, 0 ]
4199
[ "We", "found", "that", "the", "adenylate", "cyclase", "activator", ",", "forskolin", ",", "inhibits", "anti-CD3", "-induced", "shift", "in", "Lck", "electrophoretic", "mobility", ",", "suggesting", "an", "intervention", "at", "the", "TCR", "-coupled", "phosphoinositide", "turnover", "that", "precedes", "the", "activation", "of", "PKC", "." ]
[ 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]
4200
[ "The", "shift", "of", "Lck", "following", "direct", "PKC", "activation", "by", "12-O-tetradecanoyl", "phorbol", "13-acetate", ",", "which", "bypasses", "early", "receptor-triggered", "biochemical", "events", ",", "is", "insensitive", "to", "forskolin", "." ]
[ 0, 0, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]