Datasets:

---

siddharthtumre

/

Revised-JNLPBA

like 0

[ "Nevertheless", ",", "forskolin", "also", "inhibits", "PKC", "downstream", "events", ",", "such", "as", "c-jun", "expression", ",", "which", "is", "critical", "for", "the", "activation", "process", "of", "T", "cells", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "To", "further", "analyze", "potential", "cross", "points", "between", "positively", "and", "negatively", "regulating", "signaling", "pathways", "in", "T", "cells", ",", "we", "tested", "the", "effects", "of", "activators", "of", "the", "adenylate", "cyclase", "or", "PKA", "on", "two", "parallel", "mitogen-activated", "protein", "kinase", "signaling", "pathways", "mediated", "by", "extracellular", "signal-regulated", "kinase", "(", "ERK", ")", "and", "c-Jun", "N-terminal", "kinase", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 9, 10, 10, 0 ]

[ "Using", "a", "PKC", "-specific", "inhibitor", ",", "GF109203X", ",", "or", "PKC-depleted", "T", "cells", ",", "we", "found", "that", "a", "large", "part", "of", "the", "anti-CD3", "-induced", "ERK", "activation", "is", "PKC", "dependent", "." ]

[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0, 0 ]

[ "Both", "PKC-", "dependent", "and", "-independent", "activation", "of", "ERK", "were", "sensitive", "to", "inhibition", "by", "forskolin", "or", "a", "cell-permeable", "cAMP", "analogue", ",", "dbcAMP", "." ]

[ 0, 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Furthermore", ",", "the", "effect", "of", "12-O-tetradecanoyl", "phorbol", "13-acetate", "and", "ionomycin", ",", "which", "synergized", "to", "fully", "activate", "c-Jun", "N-terminal", "kinase", ",", "was", "also", "sensitive", "to", "inhibition", "by", "forskolin", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Our", "results", "suggest", "that", "PKA", "inhibits", "T", "cell", "activation", "by", "interfering", "with", "multiple", "events", "along", "the", "two", "signaling", "pathways", "operating", "downstream", "of", "the", "TCR", "and", "the", "CD28", "co-receptor", "molecules", "." ]

[ 0, 0, 0, 0, 9, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 10, 10, 0 ]

[ "Transcriptional", "and", "posttranscriptional", "regulation", "of", "erythroid", "gene", "expression", "in", "anthracycline-induced", "differentiation", "of", "human", "erythroleukemic", "cells", "." ]

[ 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0 ]

[ "Aclacinomycin", "(", "ACLA", ")", "and", "doxorubicin", "(", "DOX", ")", "were", "used", "at", "subtoxic", "concentrations", "to", "induce", "erythroid", "differentiation", "in", "the", "human", "leukemic", "cell", "line", "K562", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 5, 6, 6, 6, 6, 0 ]

[ "Cell", "hemoglobinization", "was", "accompanied", "by", "the", "increased", "expression", "of", "genes", "encoding", "gamma-globin", "and", "porphobilinogen", "deaminase", "(", "PBGD", ")", ",", "an", "enzyme", "of", "heme", "synthesis", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "By", "using", "run-on", "assays", ",", "ACLA", "was", "shown", "to", "induce", "an", "enhancement", "of", "the", "transcription", "of", "erythroid", "genes", ",", "including", "gamma-globin", ",", "PBGD", ",", "erythropoietin", "receptor", ",", "and", "GATA-1", "transcription", "factor", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 9, 0, 9, 0, 9, 10, 0, 0, 9, 10, 10, 0 ]

[ "In", "contrast", ",", "in", "DOX-treated", "cells", ",", "the", "transcription", "rate", "of", "these", "genes", "was", "unchanged", "in", "comparison", "with", "control", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "addition", ",", "inhibition", "of", "mRNA", "synthesis", "with", "actinomycin", "D", "indicated", "that", "DOX", "induced", "an", "increased", "stability", "of", "PBGD", "and", "GATA-1", "mRNAs", ",", "whereas", "ACLA", "did", "not", "affect", "the", "half-lives", "of", "these", "mRNAs", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Because", "the", "increase", "in", "erythroid", "mRNA", "steady-state", "level", "in", "anthracycline-treated", "cells", "was", "inhibited", "by", "cycloheximide", ",", "this", "suggests", "that", "transcriptional", "activation", "in", "ACLA-treated", "cells", "and", "mRNA", "stabilization", "in", "DOX-treated", "cells", "were", "dependent", "on", "de", "novo", "protein", "synthesis", "." ]

[ 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Finally", ",", "GATA-1", "protein", "level", "was", "shown", "to", "be", "increased", "in", "ACLA-treated", "but", "not", "in", "DOX-treated", "cells", "." ]

[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "These", "two", "anthracyclines", ",", "although", "closely", "related", "in", "their", "structures", ",", "appeared", "to", "act", "as", "differentiation", "inducers", "by", "distinct", "mechanisms", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Indeed", ",", "erythroid", "gene", "expression", "was", "demonstrated", "to", "be", "regulated", "transcriptionally", "by", "ACLA", "and", "mainly", "posttranscriptionally", "by", "DOX", "." ]

[ 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Interleukin-7", "signaling", "in", "human", "B", "cell", "precursor", "acute", "lymphoblastic", "leukemia", "cells", "and", "murine", "BAF3", "cells", "involves", "activation", "of", "STAT1", "and", "STAT5", "mediated", "via", "the", "interleukin-7", "receptor", "alpha", "chain", "." ]

[ 9, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 0, 5, 6, 6, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "Interleukin-7", "(", "IL-7", ")", "stimulates", "the", "proliferation", "of", "normal", "and", "leukemic", "B", "and", "T", "cell", "precursors", "and", "T", "lymphocytes", "." ]

[ 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 0, 7, 8, 0 ]

[ "Activation", "of", "the", "JAK", "/STAT", "pathway", "has", "been", "implicated", "in", "IL-7R", "signaling", "." ]

[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "We", "investigated", "which", "STAT", "complexes", "are", "formed", "upon", "stimulation", "of", "B", "cell", "precursor", "acute", "lymphoblastic", "leukemia", "(", "BCP-ALL", ")", "cells", "with", "IL-7", "." ]

[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 7, 8, 8, 8, 8, 8, 8, 8, 8, 8, 0, 9, 0 ]

[ "Gel", "retardation", "assays", "with", "STAT-binding", "oligonucleotides", "showed", "that", "IL-7", "induces", "the", "formation", "of", "two", "major", "STAT", "complexes", "in", "BCP-ALL", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 9, 10, 0, 7, 8, 0 ]

[ "Supershifts", "with", "anti-STAT", "antibodies", "identified", "these", "as", "STAT1", "and", "STAT5", "complexes", "." ]

[ 0, 0, 9, 10, 0, 0, 0, 9, 0, 9, 10, 0 ]

[ "This", "pattern", "of", "STAT", "activation", "was", "seen", "in", "all", "BCP-ALL", "cases", "that", "respond", "to", "IL-7", "in", "proliferation", "assays", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]

[ "IL-7", "also", "induced", "STAT", "/DNA", "binding", "in", "BCP-ALL", "cases", "that", "failed", "to", "proliferate", "in", "response", "to", "IL-7", ",", "suggesting", "that", "the", "ability", "of", "IL-7R", "to", "activate", "the", "JAK", "/STAT", "pathway", "per", "se", "is", "not", "sufficient", "for", "proliferation", "induction", "." ]

[ 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "To", "determine", "the", "contribution", "of", "the", "cytoplasmic", "domain", "of", "the", "IL-7", "receptor", "alpha", "chain", "(", "IL-7R", "alpha", ")", "to", "activation", "of", "STAT", "proteins", ",", "transfectants", "of", "the", "murine", "pro-B", "cell", "line", "BAF3", "were", "made", "that", "express", "chimeric", "receptors", "consisting", "of", "the", "extracellular", "domain", "of", "human", "granulocyte", "colony-stimulating", "factor", "receptor", "(", "G-CSF-R", ")", "and", "the", "transmembrane", "and", "intracellular", "domains", "of", "human", "IL-7R", "alpha", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 5, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "Activation", "of", "the", "chimeric", "G-CSF-R/IL-7R", "alpha", "with", "G-CSF", "resulted", "in", "a", "full", "proliferative", "response", "and", "induced", "the", "phosphorylation", "of", "JAK1", "but", "not", "JAK2", "." ]

[ 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0 ]

[ "Major", "STAT", "complexes", "activated", "by", "G-CSF-R/IL-7R", "alpha", "contained", "STAT1", "or", "STAT5", ",", "while", "some", "formation", "of", "STAT3-containing", "complexes", "was", "also", "seen", "." ]

[ 0, 9, 10, 0, 0, 9, 10, 0, 9, 0, 9, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0 ]

[ "These", "findings", "establish", "that", "STAT1", "and", "STAT5", ",", "and", "possibly", "STAT3", ",", "are", "activated", "upon", "stimulation", "of", "precursor", "B", "cells", "with", "IL-7", "." ]

[ 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 9, 0 ]

[ "The", "data", "further", "indicate", "that", "the", "IL-7R", "alpha", "chains", "are", "directly", "involved", "in", "the", "activation", "of", "JAKs", "and", "STATs", "and", "have", "a", "major", "role", "in", "proliferative", "signaling", "in", "precursor", "B", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "Stimulation", "of", "human", "lymphocyte", "proliferation", "and", "CD40", "antigen", "expression", "by", "phosphorothioate", "oligonucleotides", "complementary", "to", "hepatitis", "B", "virus", "genome", "." ]

[ 0, 0, 7, 8, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "have", "studied", "the", "proliferation", "and", "CD40", "antigen", "expression", "of", "lymphocytes", ",", "and", "the", "cytotoxicity", "to", "monocytes", ",", "of", "antisense", "phosphorothioate", "oligodeoxynucleotides", "complementary", "to", "the", "SP", "II", "promoter", "of", "HBV", "mRNA", "(", "sequence", "I", ")", "and", "the", "X", "gene", "(", "sequence", "II", ")", "in", "patients", "with", "chronic", "hepatitis", "B", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 7, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "oligo", "sequence", "I", "stimulated", "proliferation", "of", "both", "T", "and", ",", "to", "a", "lesser", "extent", ",", "B", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "The", "percentage", "of", "cells", "expressing", "CD40", "in", "T", "and", "B", "cell", "co-cultures", "increased", "from", "4.2", "%", "to", "13.8", "%", "after", "oligo", "stimulation", "in", "patients", ",", "while", "it", "increased", "form", "4.7", "%", "to", "48.6", "%", "in", "healthy", "controls", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "sense", "sequence", "(", "sequence", "III", ")", "of", "the", "X", "gene", "also", "enhanced", "the", "expression", "of", "CD40", "antigen", "in", "patients", "with", "hepatitis", "B", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0 ]

[ "The", "proportion", "of", "CD40", "cells", "(", "26", "%", ")", "in", "a", "resting", "B-cell", "preparation", "from", "hepatitis", "B", "patients", "decreased", "to", "zero", "after", "a", "5-day", "culture", "with", "sequence", "I", ",", "but", "IgG", "levels", "in", "the", "culture", "supernatant", "increased", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "cytotoxic", "properties", "of", "monocytes", "were", "not", "influenced", "by", "the", "oligos", "." ]

[ 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0 ]

[ "These", "findings", "indicate", "that", "antisense", "oligos", "against", "hepatitis", "B", "virus", "(", "HBV", ")", "have", "mitogenic", "effects", "on", "the", "proliferation", "of", "human", "lymphocytes", "in", "a", "non-specific", "manner", "and", "may", "activate", "T", "cells", "to", "express", "CD40", "antigen", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 9, 10, 0 ]

[ "Activation", "protein", "1", "-dependent", "transcriptional", "activation", "of", "interleukin", "2", "gene", "by", "Ca2+/calmodulin", "kinase", "type", "IV/Gr", "." ]

[ 9, 10, 10, 0, 0, 0, 0, 1, 2, 2, 0, 9, 10, 10, 10, 0 ]

[ "The", "Ca2+/calmodulin-dependent", "protein", "kinase", "(", "CaMK", ")", "type", "IV/Gr", "is", "selectively", "expressed", "in", "T", "lymphocytes", "and", "is", "activated", "after", "signaling", "via", "the", "T", "cell", "antigen", "receptor", "(", "TCR", ")", ",", "indicating", "that", "it", "mediates", "some", "of", "the", "Ca", "(", "2+", ")", "-dependent", "transcriptional", "events", "that", "follow", "TCR", "engagement", "." ]

[ 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "Here", "we", "show", "that", "CaMKIV/Gr", "induces", "the", "transcription", "factor", "activation", "protein", "1", "(", "AP-1", ")", "alone", "or", "in", "synergy", "with", "T", "cell", "mitogens", "and", "with", "the", "p21ras", "oncoprotein", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 9, 10, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 9, 10, 0 ]

[ "CaMKIV/", "Gr", "signaling", "is", "associated", "with", "transcriptional", "activation", "of", "c-fos", "but", "is", "independent", "of", "p21ras", "or", "calcineurin", "." ]

[ 9, 10, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 1, 0, 1, 0 ]

[ "AP-1", "is", "an", "integral", "component", "of", "the", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NFAT", ")", "transcriptional", "complex", ",", "which", "is", "required", "for", "interleukin", "2", "gene", "expression", "in", "T", "cells", "." ]

[ 9, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 7, 8, 0 ]

[ "We", "demonstrate", "that", "CaMKIV/Gr", "reconstitutes", "the", "capacity", "of", "the", "cytosolic", "component", "of", "NFAT", "to", "direct", "transcription", "from", "NFAT", "sites", "in", "non-", "T", "cells", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 1, 2, 0, 7, 8, 8, 0 ]

[ "These", "results", "reveal", "a", "central", "role", "for", "CaMKIV/Gr", "as", "a", "Ca", "(", "2+", ")", "-regulated", "activator", "of", "gene", "transcription", "in", "T", "lymphocytes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "IL-12", "-induced", "activation", "of", "NK", "and", "T", "cells", "occurs", "in", "the", "absence", "of", "immediate-early", "activation", "gene", "expression", "." ]

[ 9, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "responses", "of", "lymphocytes", "to", "IL-2", "and", "IL-12", ",", "involving", "proliferation", ",", "differentiation", ",", "and", "cytokine", "production", ",", "are", "only", "partially", "overlapping", ",", "and", "may", "depend", "on", "induced", "differential", "expression", "of", "specific", "sets", "of", "genes", "." ]

[ 0, 0, 0, 7, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Using", "reverse-transcription", "PCR", "differential", "display", ",", "we", "isolated", "an", "mRNA", "species", "expressed", "in", "IL-2", "-but", "not", "IL-12-stimulated", "NK", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 7, 8, 0 ]

[ "This", "was", "identified", "as", "the", "mRNA", "encoding", "the", "transcription", "factor", "egr-1", ",", "which", "is", "expressed", "with", "fast", "kinetics", "in", "T", "and", "NK", "cells", "upon", "IL-2", ",", "but", "not", "IL-12", ",", "stimulation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 9, 0, 0, 0, 9, 0, 0, 0 ]

[ "Analysis", "of", "the", "accumulation", "of", "mRNA-encoding", "members", "of", "the", "AP-1", "transcription", "factor", "family", "demonstrated", "that", "c-fos", "and", "junB", "are", "also", "expressed", "upon", "stimulation", "of", "NK", "and", "T", "cells", "with", "IL-2", ",", "but", "not", "IL-12", ",", "whereas", "expression", "of", "c-jun", "and", "junD", "is", "not", "modified", "by", "either", "cytokine", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0 ]

[ "Accordingly", ",", "increased", "AP-1", "DNA-binding", "activity", "and", "AP-1", "-dependent", "transcriptional", "activity", "were", "detected", "exclusively", "in", "IL-2-stimulated", "cells", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Analysis", "of", "the", "expression", "of", "genes", "reported", "to", "regulate", "cytokine", "-induced", "proliferation", "demonstrated", "that", "both", "IL-2", "and", "IL-12", "induce", "c-myc", "mRNA", "accumulation", "in", "NK", "and", "T", "cells", ",", "whereas", "only", "IL-2", "induces", "bcl-2", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 3, 4, 0, 0, 0, 0, 7, 8, 0, 0, 0, 9, 0, 1, 0, 0 ]

[ "Our", "data", "provide", "the", "first", "demonstration", "that", "IL-12", "-mediated", "activation", "of", "T", "and", "NK", "cells", "does", "not", "involve", "expression", "of", "members", "of", "the", "immediate-early", "activation", "genes", "family", "(", "egr-1", ",", "c-fos", ",", "and", "junB", ")", ",", "AP-1", "transcriptional", "activity", ",", "or", "bcl-2", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 1, 0, 0, 1, 0, 0, 0, 0, 1, 0, 0 ]

[ "This", "indicates", "that", "functional", "differences", "observed", "in", "IL-2-", "and", "IL-12-stimulated", "cells", "may", "depend", ",", "at", "least", "in", "part", ",", "on", "differential", "gene", "regulation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Active", "suppression", "of", "the", "class", "II", "transactivator-encoding", "AIR-1", "locus", "is", "responsible", "for", "the", "lack", "of", "major", "histocompatibility", "complex", "class", "II", "gene", "expression", "observed", "during", "differentiation", "from", "B", "cells", "to", "plasma", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 7, 8, 0, 7, 8, 0 ]

[ "In", "this", "study", "the", "genetic", "control", "of", "major", "histocompatibility", "complex", "(", "MHC", ")", "class", "II", "gene", "expression", "during", "the", "transition", "from", "B", "cell", "to", "plasma", "cell", "has", "been", "analyzed", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 7, 8, 0, 7, 8, 0, 0, 0, 0 ]

[ "Class", "II", "molecules", "are", "not", "expressed", "in", "plasma", "cells", "because", "of", "an", "active", "suppression", "resulting", "in", "the", "abrogation", "of", "class", "II", "gene", "transcription", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "show", "here", "that", "the", "plasma", "cell-specific", "repressor", "function", ",", "designated", "SIR", "(", "suppressor", "of", "immune", "response", "genes", ")", ",", "does", "not", "act", "directly", "on", "the", "transcription", "of", "class", "II", "genes", ",", "but", "instead", "on", "the", "transcription", "of", "the", "AIR-1", "gene", ",", "whose", "product", ",", "the", "class", "II", "transactivator", "(", "CIITA", ")", ",", "is", "fundamental", "for", "the", "regulation", "of", "the", "constitutive", "and", "inducible", "expression", "of", "MHC", "class", "II", "genes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]

[ "This", "was", "unambiguously", "demonstrated", "by", "the", "fact", "that", "plasmacytoma", "x", "B", "cell", "hybrids", "carrying", "an", "AIR-1", "locus", "derived", "from", "CIITA-expressing", "cells", "do", "not", "express", "CIITA-specific", "transcripts", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0 ]

[ "Transfection", "of", "a", "cDNA", "containing", "the", "human", "CIITA", "coding", "sequence", "under", "the", "control", "of", "an", "heterologous", "promoter", "restores", "expression", "of", "human", "MHC", "class", "II", "genes", "in", "the", "hybrids", "and", "is", "responsible", "for", "de", "novo", "expression", "of", "mouse", "MHC", "class", "II", "genes", "in", "both", "the", "mouse", "plasmacytoma", "cell", "line", "and", "the", "hybrids", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0 ]

[ "These", "results", "confirm", "and", "extend", "the", "notion", "of", "the", "functional", "conservation", "of", "the", "AIR-1", "gene", "product", "across", "species", "barriers", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0 ]

[ "Interestingly", ",", "in", "CIITA-transfected", "cell", "hybrids", ",", "cell", "surface", "expression", "of", "the", "human", "HLA-DQ", "heterodimer", "was", "not", "observed", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0 ]

[ "This", "result", "was", "not", "attributable", "to", "lack", "of", "HLA-DQ", "alpha", "or", "-DQ", "beta", "transcription", ",", "because", "both", "transcripts", "were", "present", "in", "the", "CIITA-transfected", "hybrids", ",", "although", "at", "reduced", "levels", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "These", "findings", "further", "support", "our", "previous", "observations", "on", "the", "distinct", "regulation", "of", "expression", "of", "the", "human", "HLA-DQ", "class", "II", "subset", ",", "which", "may", "be", "thus", "controlled", "at", "the", "posttranscriptional", "level", "by", "a", "CIITA", "-independent", "mechanism", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0 ]

[ "Requirements", "for", "induction", "of", "vitamin", "D-mediated", "gene", "regulation", "in", "normal", "human", "B", "lymphocytes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0 ]

[ "Mature", "human", "lymphocytes", "are", "unique", "targets", "of", "1", "alpha", ",", "25-dihydroxyvitamin", "D3", "(", "1", "alpha", ",", "25", "(", "OH", ")", "2D3", ")", "in", "that", "vitamin", "D", "receptors", "(", "VDR", ")", "are", "not", "constitutively", "expressed", ",", "and", "specific", "cellular", "activation", "signals", "are", "required", "for", "both", "the", "up-regulation", "of", "VDR", "and", "establishment", "of", "reactivity", "to", "the", "lipophilic", "ligand", "." ]

[ 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Treatment", "of", "B", "lymphocytes", "with", "the", "cytokine", "IL-4", "(", "IL-4", ")", ",", "in", "the", "absence", "of", "prior", "activation", ",", "induces", "a", "weak", "up-regulation", "of", "VDR", "expression", "but", "fails", "to", "generate", "vitamin", "D-responsive", "element", "(", "VDRE", ")", "-reactive", "nuclear", "protein", "complexes", "or", "to", "initiate", "the", "genomic", "transcription", "of", "25-hydroxyvitamin", "D3", "24-hydroxylase", "." ]

[ 0, 0, 7, 8, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "Stimulation", "of", "B", "lymphocytes", "by", "either", "ligation", "of", "CD40", "Ag", "or", "cross-linking", "the", "Ig", "receptor", "is", "also", "insufficient", "to", "render", "B", "lymphocytes", "responsive", "to", "1", "alpha", ",", "25", "(", "OH", ")", "2D3", "." ]

[ 0, 0, 7, 8, 0, 0, 0, 0, 9, 10, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "However", ",", "this", "apparent", "lack", "of", "response", "to", "the", "secosterol", "can", "be", "overcome", "by", "stimulation", "of", "B", "lymphocytes", "with", "a", "combination", "of", "these", "cellular", "activation", "signals", ",", "which", "are", "sufficient", "to", "lead", "to", "G1", "cell", "cycle", "progression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "the", "presence", "of", "1", "alpha", ",", "25", "(", "OH", ")", "2D3", ",", "cellular", "activation", "associated", "with", "stimulation", "of", "such", "a", "progression", "appears", "to", "be", "sufficient", "for", "the", "up-regulation", "of", "VDR", "message", "and", "protein", "and", "necessary", "for", "the", "establishment", "of", "VDRE", "binding", "complexes", "and", "the", "induction", "of", "24-hydroxylase", "message", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "Furthermore", ",", "biologic", "functions", "are", "modulated", ",", "in", "that", "the", "hormone", "inhibits", "proliferation", "in", "a", "subset", "of", "the", "activated", "B", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "These", "observations", "suggest", "that", "reactivity", "to", "1", "alpha", ",", "25", "(", "OH", ")", "2D3", "is", "tightly", "regulated", "in", "B", "lymphocytes", ",", "requiring", "specific", "signals", "for", "its", "initiation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Cross", "talk", "between", "cell", "death", "and", "cell", "cycle", "progression", ":", "BCL-2", "regulates", "NFAT", "-mediated", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0 ]

[ "BCL-2-deficient", "T", "cells", "demonstrate", "accelerated", "cell", "cycle", "progression", "and", "increased", "apoptosis", "following", "activation", "." ]

[ 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Increasing", "the", "levels", "of", "BCL-2", "retarded", "the", "G0", "--", ">", "S", "transition", ",", "sustained", "the", "levels", "of", "cyclin-dependent", "kinase", "inhibitor", "p27Kip1", ",", "and", "repressed", "postactivation", "death", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0 ]

[ "Proximal", "signal", "transduction", "events", "and", "immediate", "early", "gene", "transcription", "were", "unaffected", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "However", ",", "the", "transcription", "and", "synthesis", "of", "interleukin", "2", "and", "other", "delayed", "early", "cytokines", "were", "markedly", "attenuated", "by", "BCL-2", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "In", "contrast", ",", "a", "cysteine", "protease", "inhibitor", "that", "also", "blocks", "apoptosis", "had", "no", "substantial", "affect", "upon", "cytokine", "production", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "InterleUkin", "2", "expression", "requires", "several", "transcription", "factors", "of", "which", "nuclear", "translocation", "of", "NFAT", "(", "nuclear", "factor", "of", "activated", "T", "cells", ")", "and", "NFAT", "-mediated", "transactivation", "were", "impaired", "by", "BCL-2", "." ]

[ 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 10, 10, 10, 10, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0 ]

[ "Thus", ",", "select", "genetic", "aberrations", "in", "the", "apoptotic", "pathway", "reveal", "a", "cell", "autonomous", "coregulation", "of", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Interaction", "of", "the", "human", "T-cell", "lymphotropic", "virus", "type", "1", "tax", "transactivator", "with", "transcription", "factor", "IIA", "." ]

[ 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 9, 10, 10, 0 ]

[ "The", "Tax", "protein", "of", "human", "T-cell", "lymphotropic", "virus", "type", "1", "(", "HTLV-1", ")", "is", "a", "40-kDa", "transcriptional", "activator", "which", "is", "critical", "for", "HTLV-1", "gene", "regulation", "and", "virus-induced", "cellular", "transformation", "." ]

[ 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Tax", "is", "localized", "to", "the", "DNA", "through", "its", "interaction", "with", "the", "site-specific", "activators", "cyclic", "AMP-responsive", "element-binding", "protein", ",", "NF-kappaB", ",", "and", "serum", "response", "factor", "." ]

[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0, 9, 10, 10, 0 ]

[ "It", "has", "been", "suggested", "that", "the", "recruitment", "of", "Tax", "to", "the", "DNA", "positions", "Tax", "for", "interaction", "with", "the", "basal", "transcriptional", "machinery", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "On", "the", "basis", "of", "several", "independent", "assays", ",", "we", "now", "report", "a", "physical", "and", "functional", "interaction", "between", "Tax", "and", "the", "transcription", "factor", ",", "TFIIA", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0 ]

[ "First", ",", "Tax", "was", "found", "to", "interact", "with", "the", "35-kDa", "(", "alpha", ")", "subunit", "of", "TFIIA", "in", "the", "yeast", "two-hybrid", "interaction", "system", "." ]

[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]

[ "Importantly", ",", "two", "previously", "characterized", "mutants", "with", "point", "mutations", "in", "Tax", ",", "M32", "(", "Y196A", ",", "K197S", ")", "and", "M41", "(", "H287A", ",", "P288S", ")", ",", "which", "were", "shown", "to", "be", "defective", "in", "Tax", "-activated", "transcription", "were", "unable", "to", "interact", "with", "TFIIA", "in", "this", "assay", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]

[ "Second", ",", "a", "glutathione-S-transferase", "(", "GST", ")", "affinity-binding", "assay", "showed", "that", "the", "interaction", "of", "holo-", "TFIIA", "with", "GST-Tax", "was", "20-fold", "higher", "than", "that", "observed", "with", "either", "the", "GST-Tax", "M32", "activation", "mutant", "or", "the", "GST", "control", "." ]

[ 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "Third", ",", "a", "coimmunoprecipitation", "assay", "showed", "that", "in", "HTLV-1-infected", "human", "T", "lymphocytes", ",", "Tax", "and", "TFIIA", "were", "associated", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 9, 0, 9, 0, 0, 0 ]

[ "Finally", ",", "TFIIA", "facilitates", "Tax", "transactivation", "in", "vitro", "and", "in", "vivo", "." ]

[ 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "vitro", "transcription", "studies", "showed", "reduced", "levels", "of", "Tax", "-activated", "transcription", "in", "cell", "extracts", "depleted", "of", "TFIIA", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "In", "addition", ",", "transfection", "of", "human", "T", "lymphocytes", "with", "TFIIA", "expression", "vectors", "enhanced", "Tax", "-activated", "transcription", "of", "an", "HTLV-1", "long", "terminal", "repeat-chloramphenicol", "acetyltransferase", "reporter", "construct", "." ]

[ 0, 0, 0, 0, 0, 7, 8, 8, 0, 1, 2, 2, 0, 9, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0 ]

[ "Our", "study", "suggests", "that", "the", "interaction", "of", "Tax", "with", "the", "transcription", "factor", "TFIIA", "may", "play", "a", "role", "in", "Tax", "-mediated", "transcriptional", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]

[ "Inhibition", "of", "transcription", "factor", "Stat1", "activity", "in", "mononuclear", "cell", "cultures", "and", "T", "cells", "by", "the", "cyclic", "AMP", "signaling", "pathway", "." ]

[ 0, 0, 9, 10, 10, 0, 0, 7, 8, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0 ]

[ "Activation", "of", "T", "cells", "results", "in", "a", "cascade", "of", "gene", "activation", "and", "subsequent", "proliferation", "and", "differentiation", "into", "effector", "phenotypes", "." ]

[ 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "regulation", "of", "transcription", "factors", "belonging", "to", "the", "signal", "transducer", "and", "activator", "of", "transcription", "(", "STAT", ")", "family", "was", "analyzed", "in", "PHA-activated", "mononuclear", "cells", "and", "in", "purified", "T", "cells", "activated", "by", "cross-linking", "cell", "surface", "CD3", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 7, 8, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 9, 0 ]

[ "Cell", "activation", "resulted", "in", "a", "delayed", "induction", "of", "STAT", "DNA-binding", "activity", ",", "which", "was", "sustained", "for", "several", "days", ",", "was", "composed", "predominantly", "of", "Stat1", "and", "Stat3", ",", "and", "was", "blocked", "by", "cycloheximide", "and", "actinomycin", "D", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Increased", "Stat1", "and", "Stat3", "mRNA", "and", "protein", "levels", "were", "detected", ",", "respectively", "4", "and", "24", "h", "after", "activation", "." ]

[ 0, 3, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Stimulation", "of", "the", "cAMP", "signal", "transduction", "pathway", ",", "which", "skews", "cytokine", "production", "toward", "a", "Th2", "pattern", ",", "resulted", "in", "the", "preferential", "suppression", "of", "Stat1", "activity", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "cAMP", "inhibited", "the", "induction", "of", "expression", "of", "IL-2", "receptor", "components", ",", "but", "did", "not", "inhibit", "IL-4", "receptor", "alpha-chain", "and", "CD69", "expression", "or", "the", "induction", "of", "activator", "protein", "1", "transcription", "factors", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0 ]

[ "cAMP", "signaling", "inhibited", "Stat1", "at", "several", "different", "levels", ",", "including", "suppression", "of", "DNA", "binding", "and", "down-regulation", "of", "Stat1", "protein", "and", "mRNA", "levels", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0 ]