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2801
[ "Octamer", "independent", "activation", "of", "transcription", "from", "the", "kappa", "immunoglobulin", "germline", "promoter", "." ]
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2802
[ "Previous", "analyses", "of", "immunoglobulin", "V", "region", "promoters", "has", "led", "to", "the", "discovery", "of", "a", "common", "octamer", "motif", "which", "is", "functionally", "important", "in", "the", "tissue-specific", "and", "developmentally", "regulated", "transcriptional", "activation", "of", "immunoglobulin", "genes", "." ]
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2803
[ "The", "germline", "promoters", "(", "Ko", ")", "located", "upstream", "of", "the", "J", "region", "gene", "segments", "of", "the", "kappa", "locus", "also", "contain", "an", "octamer", "motif", "(", "containing", "a", "single", "base", "pair", "mutation", "and", "referred", "to", "as", "the", "variant", "octamer", ")", "which", "has", "been", "shown", "previously", "to", "bind", "Oct-1", "and", "Oct-2", "transcription", "factors", "in", "vitro", "." ]
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2804
[ "To", "further", "elucidate", "the", "role", "of", "this", "variant", "octamer", "motif", "in", "the", "regulation", "of", "germline", "transcription", "from", "the", "unrearranged", "kappa", "locus", ",", "we", "have", "quantitated", "the", "relative", "binding", "affinity", "of", "Oct-1", "and", "Oct-2", "for", "the", "variant", "octamer", "motif", "and", "determined", "the", "functional", "role", "of", "this", "octamer", "motif", "in", "transcriptional", "activation", "." ]
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2805
[ "We", "find", "that", ",", "although", "the", "variant", "octamer", "motif", "binds", "Oct-1", "and", "Oct-2", "in", "vitro", "with", "5-fold", "lower", "affinity", "than", "the", "consensus", "octamer", "motif", ",", "mutation", "of", "the", "variant", "octamer", "motif", "to", "either", "a", "consensus", "octamer", "or", "non-octamer", "motif", "has", "no", "effect", "on", "transcriptional", "activation", "from", "the", "germline", "promoter", "." ]
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2806
[ "We", "also", "find", "significant", "differences", "in", "activation", "of", "germline", "and", "V", "region", "promoters", "by", "kappa", "enhancers", "." ]
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2807
[ "Our", "results", "suggest", "that", "the", "germline", "promoters", "and", "V", "region", "promoters", "differ", "in", "their", "dependence", "on", "octamer", "for", "activation", "and", "respond", "differently", "to", "enhancer", "activation", "." ]
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2808
[ "These", "findings", "have", "important", "implications", "in", "regulation", "of", "germline", "transcription", "as", "well", "as", "concomitant", "activation", "of", "the", "V-J", "recombination", "of", "the", "kappa", "light", "chain", "locus", "." ]
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2809
[ "Generation", "of", "CD1+RelB+", "dendritic", "cells", "and", "tartrate-resistant", "acid", "phosphatase-positive", "osteoclast-like", "multinucleated", "giant", "cells", "from", "human", "monocytes", "." ]
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2810
[ "We", "previously", "showed", "that", "granulocyte-macrophage", "colony-stimulating", "factor", "(", "GM-CSF", ")", "and", "macrophage", "colony-stimulating", "factor", "(", "M-CSF", ")", "stimulate", "the", "differentiation", "of", "human", "monocytes", "into", "two", "phenotypically", "distinct", "types", "of", "macrophages", "." ]
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2811
[ "However", ",", "in", "vivo", ",", "not", "only", "CSF", "but", "also", "many", "other", "cytokines", "are", "produced", "under", "various", "conditions", "." ]
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2812
[ "Those", "cytokines", "may", "modulate", "the", "differentiation", "of", "monocytes", "by", "CSFs", "." ]
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2813
[ "In", "the", "present", "study", ",", "we", "showed", "that", "CD14+", "adherent", "human", "monocytes", "can", "differentiate", "into", "CD1+relB+", "dendritic", "cells", "(", "DC", ")", "by", "the", "combination", "of", "GM-CSF", "plus", "interleukin-4", "(", "IL-4", ")", "and", "that", "they", "differentiate", "into", "tartrate-resistant", "acid", "phosphatase", "(", "TRAP", ")", "-positive", "osteoclast-like", "multinucleated", "giant", "cells", "(", "MGC", ")", "by", "the", "combination", "of", "M-CSF", "plus", "IL-4", "." ]
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2814
[ "However", ",", "the", "monocyte-derived", "DC", "were", "not", "terminally", "differentiated", "cells", ";", "they", "could", "still", "convert", "to", "macrophages", "in", "response", "to", "M-CSF", "." ]
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2815
[ "Tumor", "necrosis", "factor-alpha", "(", "TNF-alpha", ")", "stimulated", "the", "terminal", "differentiation", "of", "the", "DC", "by", "downregulating", "the", "expression", "of", "the", "M-CSF", "receptor", ",", "cfms", "mRNA", ",", "and", "aborting", "the", "potential", "to", "convert", "to", "macrophages", "." ]
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2816
[ "In", "contrast", "to", "IL-4", ",", "interferon-gamma", "(", "IFN-gamma", ")", "had", "no", "demonstrable", "effect", "on", "the", "differentiation", "of", "monocytes", "." ]
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2817
[ "Rather", ",", "IFN-gamma", "antagonized", "the", "effect", "of", "IL-4", "and", "suppressed", "the", "DC", "and", "MGC", "formation", "induced", "by", "GM-CSF", "+", "IL-4", "and", "M-CSF", "+", "IL-4", ",", "respectively", "." ]
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2818
[ "Taken", "together", ",", "these", "results", "provide", "a", "new", "aspect", "to", "our", "knowledge", "of", "monocyte", "differentiation", "and", "provide", "evidence", "that", "human", "monocytes", "are", "flexible", "in", "their", "differentiation", "potential", "and", "are", "precursors", "not", "only", "of", "macrophages", "but", "also", "of", "CD1+relB+DC", "and", "TRAP-positive", "MGC", "." ]
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2819
[ "Such", "a", "diverse", "pathway", "of", "monocyte", "differentiation", "may", "constitute", "one", "of", "the", "basic", "mechanisms", "of", "immune", "regulation", "." ]
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2820
[ "The", "NF-kappa", "B", "inhibitor", ",", "tepoxalin", ",", "suppresses", "surface", "expression", "of", "the", "cell", "adhesion", "molecules", "CD62E", ",", "CD11b/CD18", "and", "CD106", "." ]
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2821
[ "Tepoxalin", ",", "a", "dual", "enzyme", "inhibitor", "of", "cyclooxygenase", "and", "5-lipoxygenase", "has", "been", "shown", "to", "inhibit", "T-cell", "activation", "." ]
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2822
[ "Its", "immunosuppressive", "property", "is", "distinct", "from", "cyclosporin", "because", "only", "tepoxalin", ",", "but", "not", "cyclosporin", ",", "suppresses", "NF-kappa", "B", "activation", "." ]
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2823
[ "Here", "we", "report", "that", "tepoxalin", "selectively", "inhibits", "intercellular", "adhesion", "molecule-1", "(", "ICAM-1", ",", "CD54", ")", "/MAC-1", "(", "CD11b/CD18", ")", "dependent", "adhesion", "of", "polymorphonuclear", "cells", "to", "IL-1", "activated", "human", "umbilical", "vein", "endothelial", "cells", "." ]
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2824
[ "The", "mechanism", "of", "inhibition", "is", "related", "to", "the", "surface", "expression", "of", "several", "cell", "adhesion", "molecules", "." ]
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2825
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2826
[ "Expression", "of", "other", "adhesion", "molecules", "such", "as", "lymphocyte", "function", "associated-antigen-1", "(", "CD11a/CD18", ")", "and", "CD54", "were", "unaffected", "." ]
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2827
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2828
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2829
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2830
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2831
[ "The", "monoclonal", "antibody", "(", "mAb", ")", "J393", "induces", "apoptosis", "in", "Jurkat", "T-cells", "." ]
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2832
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2833
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2834
[ "J393/CD43", "was", "found", "to", "be", "hyposialylated", ",", "bearing", "predominantly", "O-linked", "monosaccharide", "glycans", ",", "whereas", "G10-2/CD43", "bore", "complex", "sialylated", "tetra-", "and", "hexasaccharide", "chains", "." ]
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2835
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2836
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2837
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2838
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2839
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2840
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2841
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2842
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2844
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2846
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2847
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2848
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2849
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