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3101
[ "The", "negative", "effects", "of", "point", "mutations", "in", "the", "LMP1", "(", "1-231", ")", "core", "TRAF", "binding", "motif", "on", "TRAF", "binding", "and", "NF-kappaB", "activation", "genetically", "link", "the", "TRAFs", "to", "LMP1", "(", "1-231", ")", "-mediated", "NF-kappaB", "activation", "." ]
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3102
[ "NF-kappaB", "activation", "by", "LMP1", "(", "1-231", ")", "is", "likely", "to", "be", "mediated", "by", "TRAF1", "/TRAF2", "heteroaggregates", "since", "TRAF1", "is", "unique", "among", "the", "TRAFs", "in", "coactivating", "NF-kappaB", "with", "LMP1", "(", "1-231", ")", ",", "a", "TRAF2", "dominant-negative", "mutant", "can", "block", "LMP1", "(", "1-231", ")", "-mediated", "NF-kappaB", "activation", "as", "well", "as", "TRAF1", "coactivation", ",", "and", "30", "%", "of", "TRAF2", "is", "associated", "with", "TRAF1", "in", "EBV-transformed", "B", "cells", "." ]
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3103
[ "TRAF3", "is", "a", "negative", "modulator", "of", "LMP1", "(", "1-231", ")", "-mediated", "NF-kappaB", "activation", "." ]
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3104
[ "Surprisingly", ",", "TRAF1", ",", "-2", ",", "or", "-3", "does", "not", "interact", "with", "the", "terminal", "LMP1", "CT", "aa", "333", "to", "386", "which", "can", "independently", "mediate", "NF-kappaB", "activation", "." ]
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3105
[ "The", "constitutive", "association", "of", "TRAFs", "with", "LMP1", "through", "the", "aa", "187", "to", "231", "domain", "which", "is", "important", "in", "NF-kappaB", "activation", "and", "primary", "B-lymphocyte", "growth", "transformation", "implicates", "TRAF", "aggregation", "in", "LMP1", "signaling", "." ]
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3106
[ "Lack", "of", "IL-12", "signaling", "in", "human", "allergen-specific", "Th2", "cells", "." ]
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3107
[ "IL-12", "is", "a", "powerful", "skewer", "of", "CD4+", "T", "cell", "responses", "toward", "the", "Th1", "phenotype", "by", "inducing", "IFN-gamma", "production", "in", "naive", "Th", "cells", "." ]
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3108
[ "In", "the", "present", "study", "we", "addressed", "the", "question", "of", "whether", "IL-12", "can", "reverse", "established", "Th2", "responses", "into", "Th1/Th0", "responses", "by", "inducing", "IFN-gamma", "production", "in", "memory", "Th2", "cells", "." ]
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3109
[ "To", "this", "aim", ",", "allergen-specific", "CD4+", "T", "cell", "clones", "(", "TCC", ")", "were", "generated", "from", "the", "peripheral", "blood", "of", "three", "atopic", "patients", ",", "and", "their", "cytokine", "profiles", "were", "analyzed", "." ]
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3110
[ "The", "majority", "of", "these", "TCC", "exhibited", "a", "strongly", "polarized", "Th2", "cytokine", "profile", ",", "and", "the", "production", "of", "IFN-gamma", "could", "not", "be", "induced", "by", "exogenous", "IL-12", "." ]
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3111
[ "Only", "those", "TCC", "with", "low", "IFN-gamma", "levels", "in", "the", "absence", "of", "IL-12", "responded", "to", "IL-12", "by", "additional", "enhancement", "of", "IFN-gamma", "production", "." ]
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3112
[ "The", "IL-12", "nonresponsiveness", "of", "the", "Th2", "clones", "was", "further", "evident", "by", "the", "total", "lack", "of", "IL-12", "-induced", "phosphorylation", "of", "STAT4", "(", "signal", "transducer", "and", "activator", "of", "transcription-4", ")", ",", "a", "transcription", "factor", "that", "is", "typically", "involved", "in", "IL-12", "signaling", "." ]
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3113
[ "Consequently", ",", "IL-12", "also", "failed", "to", "induce", "the", "DNA-binding", "activity", "of", "STAT4-containing", "complexes", "in", "the", "nuclei", "of", "these", "Th2", "clones", "." ]
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3114
[ "All", "TCC", "expressed", "equal", "levels", "of", "the", "low-affinity", "IL-12R", "beta1", "subunit", "." ]
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3115
[ "Our", "results", "indicate", "that", "human", "allergen-specific", "Th", "cells", "with", "strongly", "polarized", "Th2", "cytokine", "profiles", "do", "not", "respond", "to", "IL-12", "and", ",", "therefore", ",", "can", "not", "be", "induced", "to", "produce", "IFN-gamma", "." ]
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3116
[ "The", "apparent", "high", "frequency", "of", "IL-12-nonresponsive", "Th", "cells", "within", "the", "allergen-specific", "populations", "in", "atopic", "patients", "predicts", "a", "limited", "skewing", "potential", "of", "IL-12", "in", "the", "case", "of", "established", "Th2", "responses", ",", "but", "only", "affecting", "newly", "recruited", "naive", "Th", "cells", "." ]
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3117
[ "Cloning", "and", "expression", "of", "the", "Epstein-Barr", "virus-encoded", "dUTPase", ":", "patients", "with", "acute", ",", "reactivated", "or", "chronic", "virus", "infection", "develop", "antibodies", "against", "the", "enzyme", "." ]
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3118
[ "The", "gene", "encoding", "the", "Epstein-Barr", "virus", "(", "EBV", ")", "-specific", "dUTPase", "was", "amplified", "from", "virus", "DNA", "by", "PCR", "." ]
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3119
[ "The", "active", "enzyme", "was", "expressed", "in", "Escherichia", "coli", "and", "in", "insect", "cells", "as", "a", "non-fusion", "protein", "." ]
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3120
[ "The", "protein", "from", "E.", "coli", "specifically", "converted", "dUTP", "to", "dUMP", "and", "did", "not", "react", "with", "other", "dNTPs", "or", "NTPs", "." ]
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3121
[ "Preliminary", "experiments", "yielded", "a", "Km", "value", "of", "about", "0.8", "microM", "for", "dUTP", "." ]
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3122
[ "MAbs", "against", "the", "dUTPase", "reacted", "with", "a", "protein", "of", "approximately", "31", "kDa", "in", "12-O-tetradecanoyl-phorbol-13-acetate", "(", "TPA", ")", "-stimulated", "B", "cells", "harbouring", "either", "type", "1", "or", "type", "2", "EBV", "." ]
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3123
[ "The", "protein", "was", "found", "in", "untreated", "cells", "at", "low", "levels", ",", "whereas", "induction", "of", "the", "lytic", "replication", "cycle", "by", "TPA", "treatment", "or", "by", "providing", "the", "immediate", "early", "transactivator", "BZLF1", "in", "trans", "resulted", "in", "increased", "expression", "." ]
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3124
[ "We", "demonstrated", "that", "the", "virus", "dUTPase", "isolated", "from", "EBV-infected", "cells", "is", "a", "phosphoprotein", "." ]
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3125
[ "The", "protein", "expressed", "in", "insect", "cells", "was", "used", "to", "test", "for", "the", "presence", "of", "specific", "antibodies", "in", "sera", "from", "normal", ",", "healthy", "carriers", "and", "from", "patients", "with", "various", "diseases", "." ]
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3126
[ "While", "the", "sera", "of", "EBV-negative", "individuals", "(", "0/3", ")", "or", "healthy", "carriers", "(", "0/33", ")", "did", "not", "contain", "detectable", "levels", "of", "antibodies", ",", "patients", "with", "mononucleosis", "(", "5/18", ")", ",", "chronic", "EBV", "infection", "(", "2/7", ")", ",", "EBV", "reactivation", "(", "7/20", ")", "and", "human", "immunodeficiency", "virus", "infection", "(", "5/24", ")", "showed", "elevated", "antibody", "titres", "against", "the", "enzyme", "." ]
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3127
[ "This", "indicated", "that", "the", "dUTPase", "is", "expressed", "during", "EBV", "replication", "and", "reactivation", "." ]
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3128
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3129
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3130
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3131
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3132
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3133
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3134
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3135
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3136
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3137
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3138
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3139
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3140
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3141
[ "Their", "corresponding", "plasma", "cortisol", "levels", "were", "assessed", "." ]
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3142
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3143
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3144
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3145
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3146
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3147
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3148
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3149
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3150
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3151
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3152
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