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17501
[ "Interferon", "affects", "nuclear", "proteins", "in", "cells", "of", "clinically", "sensitive", "chronic", "myelogenous", "leukemia", "patients", "." ]
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17502
[ "Cytoplasmic", "protein", "extracts", "from", "chronic", "myelogenous", "leukemia", "(", "CML", ")", "cells", "contained", "an", "activity", "that", "altered", "the", "electrophoretic", "mobility", "of", "complexes", "formed", "between", "nuclear", "proteins", "and", "the", "transcriptional", "enhancers", "of", "interferon", "(", "IFN", ")", "-inducible", "genes", "." ]
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17503
[ "Exposure", "of", "CML", "cells", "to", "IFN-alpha", "diminished", "the", "effect", "of", "the", "CML", "cytoplasmic", "proteins", "on", "these", "nuclear", "protein-DNA", "complexes", "." ]
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17504
[ "The", "presence", "of", "clinical", "responsiveness", "to", "IFN-alpha", "correlated", "with", "the", "sensitivity", "to", "the", "IFN", "-induced", "change", "in", "the", "electrophoretic", "mobility", "of", "nuclear", "protein-DNA", "complexes", "." ]
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17505
[ "These", "data", "suggest", "that", "the", "action", "of", "IFN-alpha", "in", "CML", "may", "be", "linked", "to", "a", "pathway", "that", "can", "result", "in", "posttranslational", "modification", "of", "nuclear", "proteins", "." ]
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17506
[ "Epstein-Barr", "virus", "nuclear", "antigen", "2", "transactivates", "latent", "membrane", "protein", "LMP1", "." ]
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17507
[ "Several", "lines", "of", "evidence", "are", "compatible", "with", "the", "hypothesis", "that", "Epstein-Barr", "virus", "(", "EBV", ")", "nuclear", "antigen", "2", "(", "EBNA-2", ")", "or", "leader", "protein", "(", "EBNA-LP", ")", "affects", "expression", "of", "the", "EBV", "latent", "infection", "membrane", "protein", "LMP1", "." ]
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17508
[ "We", "now", "demonstrate", "the", "following", "." ]
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17509
[ "(", "i", ")", "Acute", "transfection", "and", "expression", "of", "EBNA-2", "under", "control", "of", "simian", "virus", "40", "or", "Moloney", "murine", "leukemia", "virus", "promoters", "resulted", "in", "increased", "LMP1", "expression", "in", "P3HR-1-infected", "Burkitt", "'s", "lymphoma", "cells", "and", "the", "P3HR-1", "or", "Daudi", "cell", "line", "." ]
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17510
[ "(", "ii", ")", "Transfection", "and", "expression", "of", "EBNA-LP", "alone", "had", "no", "effect", "on", "LMP1", "expression", "and", "did", "not", "act", "synergistically", "with", "EBNA-2", "to", "affect", "LMP1", "expression", "." ]
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17511
[ "(", "iii", ")", "LMP1", "expression", "in", "Daudi", "and", "P3HR-1-infected", "cells", "was", "controlled", "at", "the", "mRNA", "level", ",", "and", "EBNA-2", "expression", "in", "Daudi", "cells", "increased", "LMP1", "mRNA", "." ]
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17512
[ "(", "iv", ")", "No", "other", "EBV", "genes", "were", "required", "for", "EBNA-2", "transactivation", "of", "LMP1", "since", "cotransfection", "of", "recombinant", "EBNA-2", "expression", "vectors", "and", "genomic", "LMP1", "DNA", "fragments", "enhanced", "LMP1", "expression", "in", "the", "EBV-negative", "B-lymphoma", "cell", "lines", "BJAB", ",", "Louckes", ",", "and", "BL30", "." ]
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17513
[ "(", "v", ")", "An", "EBNA-2-responsive", "element", "was", "found", "within", "the", "-512", "to", "+40", "LMP1", "DNA", "since", "this", "DNA", "linked", "to", "a", "chloramphenicol", "acetyltransferase", "reporter", "gene", "was", "transactivated", "by", "cotransfection", "with", "an", "EBNA-2", "expression", "vector", "." ]
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17514
[ "(", "vi", ")", "The", "EBV", "type", "2", "EBNA-2", "transactivated", "LMP1", "as", "well", "as", "the", "EBV", "type", "1", "EBNA-2", "." ]
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17515
[ "(", "vii", ")", "Two", "deletions", "within", "the", "EBNA-2", "gene", "which", "rendered", "EBV", "transformation", "incompetent", "did", "not", "transactivate", "LMP1", ",", "whereas", "a", "transformation-competent", "EBNA-2", "deletion", "mutant", "did", "transactivate", "LMP1", "." ]
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17516
[ "LMP1", "is", "a", "potent", "effector", "of", "B-lymphocyte", "activation", "and", "can", "act", "synergistically", "with", "EBNA-2", "to", "induce", "cellular", "CD23", "gene", "expression", "." ]
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17517
[ "Thus", ",", "EBNA-2", "transactivation", "of", "LMP1", "amplifies", "the", "biological", "impact", "of", "EBNA-2", "and", "underscores", "its", "central", "role", "in", "EBV-induced", "growth", "transformation", "." ]
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17518
[ "The", "expression", "of", "c-fos", ",", "c-jun", ",", "and", "c-myc", "genes", "is", "regulated", "by", "heat", "shock", "in", "human", "lymphoid", "cells", "." ]
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17519
[ "The", "effect", "of", "heat", "shock", "on", "the", "expression", "of", "the", "nuclear", "protooncogenes", "c-fos", ",", "c-jun", ",", "and", "c-myc", "was", "studied", "in", "human", "lymphoid", "cells", "." ]
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17520
[ "Heat", "shock", "caused", "an", "increase", "in", "c-fos", "and", "c-jun", "mRNA", "levels", "and", "a", "decrease", "in", "c-myc", "mRNA", "levels", "in", "pre-B", "(", "Hyon", ")", "and", "T", "(", "DND-41", ")", "cell", "lines", "as", "well", "as", "in", "freshly", "isolated", "normal", "human", "thymocytes", "." ]
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17521
[ "The", "changes", "in", "the", "mRNA", "levels", "of", "these", "protooncogenes", "in", "Hyon", "cells", "were", "most", "pronounced", "at", "42", "and", "43", "degrees", "C", ";", "kinetic", "analysis", "demonstrated", "that", "the", "changes", "could", "be", "detected", "within", "30", "min", "of", "heat", "shock", "." ]
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17522
[ "Altered", "transcription", "of", "c-fos", "and", "c-myc", "genes", "was", "the", "primary", "effect", "of", "heat", "shock", "." ]
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17523
[ "Secondarily", ",", "heat", "shock", "of", "Hyon", "cells", "stabilized", "the", "c-myc", "mRNA", "level", "by", "increasing", "its", "half-life", "from", "24", "to", "45", "min", "." ]
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17524
[ "The", "overall", "effect", "of", "heat", "shock", "on", "c-myc", "mRNA", "level", ",", "however", ",", "was", "a", "marked", "inhibition", "of", "its", "transcription", "." ]
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17525
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17526
[ "Mapping", "of", "B-cell", "epitopes", "of", "the", "human", "hepatitis", "B", "virus", "X", "protein", "." ]
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17527
[ "The", "immune", "response", "to", "the", "X", "protein", "of", "human", "hepatitis", "B", "virus", "(", "HBV", ")", "was", "studied", "by", "epitope", "mapping", "by", "using", "a", "set", "of", "MS2-HBx", "fusion", "proteins", "and", "synthetic", "peptides", "." ]
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17528
[ "Antibodies", "in", "sera", "of", "patients", "with", "acute", "and", "chronic", "HBV", "infection", "showed", "a", "multispecific", "immune", "response", "." ]
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17529
[ "Each", "serum", "contained", "antibodies", "to", "a", "different", "set", "of", "epitopes", ",", "which", "taken", "together", "cover", "most", "of", "the", "HBx", "sequence", "." ]
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17530
[ "Some", "of", "the", "epitopes", "were", "detectable", "only", "by", "immunoblotting", "with", "fusion", "proteins", ";", "others", "were", "detectable", "only", "by", "an", "enzyme-linked", "immunosorbent", "assay", "(", "ELISA", ")", "with", "synthetic", "peptides", "." ]
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17531
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17532
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17533
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17534
[ "Such", "tests", "may", "become", "useful", "diagnostic", "tools", "." ]
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17535
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17536
[ "Random", "migration", "(", "RM", ")", "and", "chemotactic", "migration", "(", "ChtM", ")", "of", "human", "leukocytes", "to", "yeast", "activated", "serum", "were", "studied", "with", "the", "modified", "Boyden", "chamber", "method", "." ]
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17537
[ "Both", "RM", "and", "ChtM", "showed", "circadian", "rhythm", "." ]
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17538
[ "Leukocytes", "migrated", "most", "rapidly", "at", "night", "." ]
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17539
[ "The", "difference", "between", "the", "peak", "(", "0", ":", "00", ")", "and", "trough", "values", "(", "8", ":", "00", ")", "of", "RM", "and", "ChtM", "was", "significant", "statistically", "(", "P", "less", "than", "0.01", ")", "." ]
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17540
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17541
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17542
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17543
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17544
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17545
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17546
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17547
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17548
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17549
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17550
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17551
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17552
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17553
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17554
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17555
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17556
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17557
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17568
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17569
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17570
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17575
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17576
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17579
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17580
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17581
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17582
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17583
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17585
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17586
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17589
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17591
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17593
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17594
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