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16501
[ "M-CSF", "treatment", "was", "also", "associated", "with", "a", "rapid", "induction", "of", "the", "jun-B", "gene", ",", "although", "expression", "of", "this", "gene", "was", "prolonged", "compared", "to", "that", "of", "c-jun", "." ]
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16502
[ "We", "further", "demonstrate", "that", "M-CSF", "increases", "c-fos", "mRNA", "levels", "in", "human", "monocytes", "through", "control", "at", "both", "the", "transcriptional", "and", "posttranscriptional", "levels", "." ]
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16503
[ "Maximal", "induction", "of", "the", "c-fos", "gene", "was", "followed", "by", "that", "for", "the", "fos-B", "gene", "." ]
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16504
[ "Moreover", ",", "M-CSF", "-induced", "expression", "of", "the", "fos-related", "gene", ",", "fra-1", ",", "was", "delayed", "compared", "to", "that", "for", "both", "c-fos", "and", "fos-B", "." ]
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16505
[ "Taken", "together", ",", "the", "results", "indicate", "that", "M-CSF", "treatment", "is", "associated", "with", "differential", "activation", "of", "multiple", "members", "of", "the", "jun/fos", "family", "and", "that", "expression", "of", "these", "genes", "could", "contribute", "to", "nuclear", "signaling", "mechanisms", "that", "regulate", "a", "specific", "program", "of", "monocyte", "differentiation", "." ]
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16506
[ "Glucocorticoid", "receptors", "in", "systemic", "lupus", "erythematosus", "." ]
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16507
[ "Glucocorticosteroids", "remain", "the", "major", "treatment", "modality", "for", "systemic", "lupus", "erythematosus", "(", "SLE", ")", ",", "but", "their", "mechanism", "of", "action", "is", "unclear", "." ]
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16508
[ "Over", "the", "past", "decade", "it", "has", "become", "clear", "that", "glucocorticosteroid", "receptors", "play", "a", "significant", "role", "in", "the", "mechanism", "of", "glucocorticosteroid", "action", "." ]
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16509
[ "We", "studied", "glucocorticosteroid", "receptor", "density", "and", "affinity", "on", "peripheral", "blood", "mononuclear", "cells", "by", "the", "glucocorticosteroid", "binding", "assay", "in", "33", "patients", "with", "SLE", "who", "had", "taken", "no", "glucocorticosteroid", "for", "the", "previous", "6", "months", "and", "in", "32", "healthy", "controls", "." ]
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16510
[ "Patients", "'", "disease", "activity", "was", "measured", "by", "the", "SLE", "Disease", "Activity", "Index", "(", "SLEDAI", ")", "." ]
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16511
[ "Glucocorticosteroid", "receptors", "on", "leukocytes", "of", "patients", "with", "SLE", "were", "significantly", "higher", "than", "in", "healthy", "controls", "(", "4419", "+/-", "306", "vs", "3369", "+/-", "196", ",", "p", "less", "than", "0.005", ")", "." ]
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16512
[ "The", "binding", "affinity", "was", "not", "different", "between", "patients", "and", "controls", "." ]
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16513
[ "There", "was", "no", "correlation", "between", "glucocorticosteroid", "receptor", "number", "and", "SLE", "disease", "activity", "." ]
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16514
[ "Kappa", "B", "binding", "proteins", "are", "constitutively", "expressed", "in", "an", "IL-2", "autocrine", "human", "T", "cell", "line", "." ]
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16515
[ "The", "IL-2", "and", "the", "IL-2-R", "alpha", "genes", "are", "both", "expressed", "transiently", "in", "normal", "T", "lymphocytes", "after", "Ag", "or", "mitogen", "activation", "." ]
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16516
[ "In", "contrast", ",", "the", "human", "T", "cell", "line", ",", "IARC", "301", ",", "expresses", "these", "two", "genes", "constitutively", "and", "we", "have", "previously", "demonstrated", "that", "its", "growth", "depends", "on", "the", "autocrine", "production", "of", "this", "T", "cell", "growth", "factor", "and", "high", "affinity", "IL-2R", "." ]
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16517
[ "To", "dissect", "the", "molecular", "basis", "for", "the", "unusual", "persistent", "expression", "of", "the", "IL-2", "and", "IL-2-R", "alpha", "genes", "in", "these", "IARC", "301", "T", "cells", ",", "we", "have", "analyzed", "the", "interactions", "of", "constitutively", "expressed", "nuclear", "proteins", "with", "the", "5", "'", "flanking", "regions", "of", "the", "IL-2", "and", "IL-2-R", "alpha", "genes", "using", "both", "DNase", "I", "footprinting", "and", "gel", "retardation", "techniques", "." ]
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16518
[ "We", "have", "found", "that", "a", "region", "in", "both", "genes", "(", "-276", "to", "-250", "for", "IL-2-R", "alpha", "and", "-203", "to", "-183", "for", "IL-2", ")", ",", "which", "corresponds", "to", "a", "kappa", "B", "enhancer", "element", ",", "is", "specifically", "protected", "by", "nuclear", "proteins", "from", "IARC", "301", "." ]
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16519
[ "In", "agreement", "with", "this", "finding", ",", "both", "the", "IL-2", "and", "IL-2-R", "alpha", "promoters", "are", "active", "in", "transient", "transfection", "assays", "in", "IARC", "301", "cells", "." ]
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16520
[ "In", "contrast", ",", "mutation", "of", "the", "kappa", "B", "enhancer", "results", "in", "markedly", "attenuated", "activities", "of", "both", "promoters", "." ]
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16521
[ "Two", "proteins", "binding", "the", "kappa", "B", "sequence", ",", "NF-kappa", "B", "and", "KBF1", ",", "are", "constitutively", "expressed", "in", "IARC", "301", "nuclei", "and", "induced", "by", "PMA", "and", "PHA", "in", "Jurkat", "." ]
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16522
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16523
[ "HIV1", "infection", "of", "human", "monocytes", "and", "macrophages", "promotes", "induction", "or", "translocation", "of", "NF-KB-related", "factors", "." ]
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16524
[ "In", "1991", ",", "we", "demonstrated", ",", "using", "electrophoretic", "mobility", "shift", "assays", ",", "that", "3", "different", "factors", "(", "termed", "B1", ",", "B2", "and", "B3", ")", "with", "affinity", "for", "the", "KB-enhancer", "target", "sequence", "were", "specifically", "detected", "in", "nuclear", "extracts", "from", "HIV1-infected", "monocytes", "and", "macrophages", "." ]
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16525
[ "The", "B2", "factor", "was", "induced", "in", "the", "nuclei", "of", "these", "cells", "only", "upon", "HIV1", "infection", "." ]
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16526
[ "The", "B3", "factor", "was", "only", "slightly", "evident", "in", "nuclei", "of", "uninfected", "cells", "but", "was", "readily", "detectable", "in", "nuclei", "of", "infected", "monocytes", "." ]
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16527
[ "Its", "expression", "remained", "very", "low", "in", "nuclei", "of", "HIV1-infected", "macrophages", "." ]
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16528
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16529
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16530
[ "The", "B3", "factor", "is", "detected", "in", "the", "cytosol", "only", "when", "cells", "are", "HIV1-infected", "." ]
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16531
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16532
[ "Identification", "and", "cloning", "of", "TCF-1", ",", "a", "T", "lymphocyte-specific", "transcription", "factor", "containing", "a", "sequence-specific", "HMG", "box", "." ]
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16533
[ "CD3", "-epsilon", "expression", "is", "controlled", "by", "a", "downstream", "T", "lymphocyte-specific", "enhancer", "element", "." ]
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16534
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16535
[ "The", "multimerized", "recognition", "motif", "of", "TCF-1", "constituted", "a", "T", "cell-specific", "enhancer", "." ]
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16536
[ "Subsequent", "cloning", "of", "TCF-1", "identified", "three", "splice", "alternatives", "." ]
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16537
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16538
[ "TCF-1", "mRNA", "was", "expressed", "uniquely", "in", "T", "lymphocytes", "." ]
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16539
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16540
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16541
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16542
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16543
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16544
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16545
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16546
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16547
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16548
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16549
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16550
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16551
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16552
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16553
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16554
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16555
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16556
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16557
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16558
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16559
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16560
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16561
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16562
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16563
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16580
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16581
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16583
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16584
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16595
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