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Unusual eye injuries.
The analysis of ten unusual eye injuries, and the discussion of appropriate preventive measures. Ten patients hospitalized at the Clinic of Eye Diseases, Clinical Center of Serbia, Belgrade, in the period from January 2000. to December 2009. were presented. Following data were taken in account: sex and age of the patient, injured eye, the mechanism of injury, type of the injury, applied treatment and final visual acuity. The circumstances of the eye injuring of ten patients were described. The eye injuries in all cases occurred in a bizarre way and they were severe enough to require hospital admission. The surgery was necessary in seven patients. Three injured eyes resulted in blindness, and two additional had subnormal vision. In three quarters of discussed cases injuries can be avoided. It is necessary to implement protective measures constantly and consistently and to think about the possible consequences of certain activities. Particular attention should be paid on safety of children. The iatrogenic injuries require special consideration. | {
"pile_set_name": "PubMed Abstracts"
} |
Callose Synthase Family Genes Involved in the Grapevine Defense Response to Downy Mildew Disease.
The deposition of callose is a common plant defense response to intruding pathogens and part of the plant's innate immunity. In this study, eight grapevine callose synthase (CalS) genes were identified and characterized. To investigate biological function of CalS in grapevine against the infection of Plasmopara viticola, expression patterns of grapevine CalS family genes were analyzed among resistant/susceptible cultivars. After P. viticola infection, expression of CalS1, 3, 7, 8, 9, 10, and 11 were significantly modified among the grapevine cultivars. For example, the expression of CalS1 and CalS10 were greatly increased in downy mildew (DM)-immune Muscadinia rotundifolia 'Carlos' and 'Noble'. Transient expression assay with promoters of the CalS1 and CalS10 genes confirmed that they were regulated by the oomycete pathogen P. viticola. CalS1 promoter activity was also significantly up-regulated by ABA in DM-immune M. rotundifolia 'Noble', but down-regulated in DM-susceptible Vitis vinifera 'Chardonnay'. The CalS1 promoter, however, was also down-regulated by GA in 'Chardonnay', but not affected in 'Noble'. The promoter activity of CalS10 was significantly up-regulated by GA in 'Chardonnay', but not regulated by ABA at all. It is proposed that CalS1 and CalS10 were involved in grapevine defense against DM disease. | {
"pile_set_name": "PubMed Abstracts"
} |
Increasing feasibility and utility of (18)F-FDOPA PET for the management of glioma.
Despite radical treatment therapies, glioma continues to carry with it a uniformly poor prognosis. Patients diagnosed with WHO Grade IV glioma (glioblastomas; GBM) generally succumb within two years, even those with WHO Grade III anaplastic gliomas and WHO Grade II gliomas carry prognoses of 2-5 and 2 years, respectively. PET imaging with (18)F-FDOPA allows in vivo assessment of the metabolism of glioma relative to surrounding tissues. The high sensitivity of (18)F-DOPA imaging grants utility for a number of clinical applications. A collection of published work about (18)F-FDOPA PET was made and a critical review was discussed and written. A number of research papers have been published demonstrating that in conjunction with MRI, (18)F-FDOPA PET provides greater sensitivity and specificity than these modalities in detection, grading, prognosis and validation of treatment success in both primary and recurrent gliomas. In further comparisons with (11)C-MET, (18)F-FLT, (18)F-FET and MRI, (18)F-FDOPA has shown similar or better efficacy. Recently synthesis cassettes have become available, making (18)F-FDOPA more accessible. According to the available data, (18)F-FDOPA PET is a viable radiotracer for imaging and treatment planning of gliomas. (18)F-FDOPA PET appears to be a viable radiopharmaceutical for the diagnosis and treatment planning of gliomas cases, improving on that of MRI and (18)F-FDG PET. | {
"pile_set_name": "PubMed Abstracts"
} |
Simultaneous separation by high-performance liquid chromatography of carbamoyl aspartate, carbamoyl phosphate and dihydroorotic acid.
Leflunomide is an immunomodulatory drug which acts by inhibiting dihydroorotic acid dehydrogenase, the fourth enzyme of pyrimidine biosynthesis. We modified our high-performance liquid chromatography method to demonstrate that the principal metabolite in mitogen-stimulated human T-lymphocytes incubated with leflunomide was not dihydroorotic acid, but carbamoyl aspartate. Identification involved preparation of [14C]carbamoyl aspartate from [14C]aspartic acid and mammalian aspartate transcarbamoylase. Accumulation of carbamoyl aspartate indicates that under these conditions the equilibrium constant for dihydroorotase favours the reverse reaction. This HPLC method, enabling simultaneous separation of the first four intermediates in the de novo pyrimidine pathway may be of use in a variety of experimental situations. | {
"pile_set_name": "PubMed Abstracts"
} |
Stem cells and regenerative medicine for neural repair.
Clinical trials of cell-based therapies that use pluripotent stem cells (PSC) have already started for several neurological diseases including spinal cord injury and age-related macular degeneration. Regarding future PSC-based clinical trials for other neurological diseases, these trials have been instrumental at recognizing first, the difference between research cell lines and clinical cell lines of a stem cell product, second, the selection of an appropriate animal model for pre-clinical study, third, criteria and the quality control of donor cells, and fourth, the mode of action of the grafts. | {
"pile_set_name": "PubMed Abstracts"
} |
Compared toxicity of the potassium channel blockers, apamin and dendrotoxin.
The central toxicities of two potassium ion channel blockers, apamin and alpha-dendrotoxin (DTx), have been compared. Both apamin and dendrotoxin injected intracerebroventricularly produced signs of poisoning, including tremor and ataxia; however, only DTx produced changes in brain electrical activity, with high voltage spikes and epileptiform activity and subsequent brain damage. DTx, but not apamin, increased the amplitude of evoked field potentials and caused repetitive firing of neurones in hippocampal slices. Signs of poisoning following peripheral (intraperitoneal) administration of apamin were similar to those following central administration, including dramatic haemorrhagic effects on the lungs of decedent animals. These results are consistent with dendrotoxin being a centrally-active neurotoxin producing epileptiform activity and brain damage, whilst apamin produces its most significant pathology in the lung, possibly involving a neurogenic mechanism. | {
"pile_set_name": "PubMed Abstracts"
} |
Limonoids from Citrus sudachi.
Four new limonoid derivatives, 1-O-methylichangensin (1), sudachinoid A (2), B (3), and C (4) were isolated from the seeds of Citrus sudachi, together with the known compounds ichangensin, obacunone, obacunoic acid and limonin, and the structures of the new compounds were elucidated based on spectroscopic and chemical evidence. | {
"pile_set_name": "PubMed Abstracts"
} |
Scalable nanostructured membranes for solid-oxide fuel cells.
The use of oxide fuel cells and other solid-state ionic devices in energy applications is limited by their requirement for elevated operating temperatures, typically above 800°C (ref. 1). Thin-film membranes allow low-temperature operation by reducing the ohmic resistance of the electrolytes. However, although proof-of-concept thin-film devices have been demonstrated, scaling up remains a significant challenge because large-area membranes less than ~ 100 nm thick are susceptible to mechanical failure. Here, we report that nanoscale yttria-stabilized zirconia membranes with lateral dimensions on the scale of millimetres or centimetres can be made thermomechanically stable by depositing metallic grids on them to function as mechanical supports. We combine such a membrane with a nanostructured dense oxide cathode to make a thin-film solid-oxide fuel cell that can achieve a power density of 155 mW cm⁻² at 510 °C. We also report a total power output of more than 20 mW from a single fuel-cell chip. Our large-area membranes could also be relevant to electrochemical energy applications such as gas separation, hydrogen production and permeation membranes. | {
"pile_set_name": "PubMed Abstracts"
} |
Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine.
In prokaryotes, incomplete or misfolded polypeptides emanating from a stalled ribosome are marked for degradation by the addition of an 11 residue peptide (AANDENYALAA) to their C terminus. Substrates containing this conserved degradation signal, the SsrA tag, are targeted to specific proteases including ClpXP and ClpAP. SspB was originally characterized as a stringent starvation protein and has been found to bind specifically to SsrA-tagged proteins and to enhance recognition of these proteins by the ClpXP degradation machine. Here, we report the crystal structures of SspB alone and in complex with an SsrA peptide. Unexpectedly, SspB exhibits a fold found in Sm-family RNA binding proteins. The dimeric SspB structures explain the key determinants for recognition of the SsrA tag and define a hydrophobic channel that may bind unfolded substrates. | {
"pile_set_name": "PubMed Abstracts"
} |
Economical multiple-site intradermal immunisation with human diploid-cell-strain vaccine is effective for post-exposure rabies prophylaxis.
An economical post-exposure regimen of Mérieux human diploid-cell-strain vaccine (HDCSV) was compared with Semple vaccine (SV), the most widely used vaccine in Asia. 155 patients bitten by animals proved to be rabid received either conventional courses of SV (34 severe and 43 mild cases) or HDCSV, 0.1 ml intradermally, at eight sites on day 0, at four sites on day 7, and at one site on days 28 and 91 (36 severe and 42 mild cases). All severely bitten patients were given equine anti-rabies serum (EARS), 80 IU/kg on day 0. There were no deaths from rabies in either group. Follow-up was 97.5% at 1 year and 93% at 2 years. 88% of patients given HDCSV alone had detectable neutralising antibody on day 7 in contrast to 2% given SV alone. Antibody persisted until 1 year in all sera tested from HDCSV patients in contrast to only 48% of SV sera. The high dose of EARS resulted in pronounced suppression of response to HDCSV. There were no serious systemic side-effects but local side-effects were significantly more common in the SV group. The multiple-site intradermal HDCSV regimen was at least as effective as SV. The amount of HDCSV used was 30% of the conventional dose. | {
"pile_set_name": "PubMed Abstracts"
} |
A new crystal form of the complex between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus that diffracts to 2.8 A resolution.
Two distinct complexes between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus have been crystallized using ammonium sulphate as a precipitant. Form III crystals grow from solutions containing a 1:2.5 stoichiometry of synthetase dimer to tRNA. They are of monoclinic space group C2 with unit cell dimensions a = 211.6 A, b = 126.8 A, c = 197.1 A, beta = 132.4 degrees and diffract to about 3.5 A. Preliminary crystallographic results show that the crystallographic asymmetric unit contains two synthetase dimers. Form IV crystals grow from solutions containing a 1:1.5 stoichiometry of synthetase dimer to tRNA. They are of orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 124.5 A, b = 128.9 A, c = 121.2 A and diffract to 2.8 A resolution. Preliminary crystallographic results show that these crystals contain only one tRNA molecule bound to a synthetase dimer. | {
"pile_set_name": "PubMed Abstracts"
} |
[New trends in prognosis and treatment of acute pancreatitis].
Severe acute pancreatitis is common and potentially fatal disease with nonspecific treatment and often unpredictable prognosis. Early, precise discrimination between patients likely and unlikely to have severe pancreatitis would be of great benefit in establishing proper therapy. Evidence based pharmacological treatment of AP is limited and studies on the effect of potent anti-inflammatory drugs are warranted. Experimental data with new anti-inflammatory, and/or immunomodulatory drugs seems to be promising. CRAI (continous regional arterial infusion) with a proteinase inhibitor is a new method for treatment of necrotizing pancreatitis, which needs further evaluation. In this review attention was focused on the progress and new directions in prognosis of severity and treatment of acute pancreatitis. | {
"pile_set_name": "PubMed Abstracts"
} |
Changes in the ratio of urinary alpha 1-microglobulin to ulinastatin levels in patients with Alzheimer-type dementia and vascular dementia.
Relationships between urinary levels of alpha 1-microglobulin (alpha 1M) and ulinastatin (UT) in patients with dementia were investigated. There were no significant differences in alpha 1M and UT levels and alpha 1M: UT ratios among three groups: age-matched control subjects, patients with either Alzheimer-type senile dementia (ATD) or vascular dementia (VD). Although a positive correlation was established between alpha 1M and UT levels in these groups, the regression of the demented patients differed significantly from that of controls (P < 0.05). A tendency towards a negative correlation between alpha 1M: UT ratios and the levels of severity or duration of the disease was displayed in the ATD group, whereas a tendency toward a positive correlation between alpha 1M: UT ratios and the levels of severity was observed in the VD group. These results suggest that changes in the relationships between urinary levels of alpha 1M and UT may provide a useful biochemical index for diagnoses of ATD and VD. | {
"pile_set_name": "PubMed Abstracts"
} |
Longitudinal assessment of plus disease in retinopathy of prematurity using color Doppler imaging.
Retinal vascular changes and the development of plus disease are the hallmarks of retinopathy of prematurity (ROP). The purpose of this study was to evaluate whether or not serial examinations of retrobulbar blood flow characteristics, as measured by color Doppler imaging (CDI) performed repeatedly over a period of several weeks, would be useful for predicting those infants at risk for developing plus disease and to determine whether this technique may be used as an objective tool for confirming the presence of plus disease. Of the 73 infants followed in this study, 14 (19%) developed plus disease confirmed by a panel of experts. When comparing the group of infants developing plus disease with those infants who did not develop plus disease, we did not find any significant differences in the retrobulbar blood flow characteristics of either the central retinal or ophthalmic arteries. Color Doppler imaging did not appear to be a clinically useful tool in the longitudinal management of ROP, nor did it appear to be useful as an objective determinant of plus disease in these premature infants. | {
"pile_set_name": "PubMed Abstracts"
} |
Deep brain stimulation of the centromedian thalamic nucleus for the treatment of generalized and frontal epilepsies.
Deep brain stimulation (DBS) of the thalamus is an emerging surgical option for people with medically refractory epilepsy that is not suitable for resective surgery, or in whom surgery has failed. Our main aim was to evaluate the efficacy of bilateral centromedian thalamic nucleus (CMN) DBS for seizure control in generalized epilepsy and frontal lobe epilepsy with a two-center, single-blind, controlled trial. Participants were adults with refractory generalized or frontal lobe epilepsy. Seizure diaries were kept by patients/carers prospectively from enrollment. The baseline preimplantation period was followed by a control period consisting of a blind stimulation-OFF phase of at least 3 months, a 3-month blind stimulation-ON phase, and a 6-month unblinded stimulation-ON phase. The control period was followed by an unblinded long-term extension phase with stimulation-ON in those patients in whom stimulation was thought to be effective. Eleven patients were recruited at King's College Hospital (London, United Kingdom United Kingdom) and at University Hospital La Princesa (Madrid, Spain). Among the five patients with frontal lobe epilepsy, only one patient had >50% improvement in seizure frequency during the blind period. In the long-term extension phase, two patients with frontal lobe epilepsy had >50% improvement in seizure frequency. All six patients with generalized epilepsy had >50% improvement in seizure frequency during the blind period. In the long-term extension phase, five of the six patients showed >50% improvement in the frequency of major seizures (one became seizure free, one had >99% improvement, and three had 60-95% reduction in seizure frequency). Among patients with generalized epilepsy, the DBS implantation itself appears to be effective, as two patients remained seizure free during 12 and 50 months with DBS OFF, and the remaining four had 50-91% improvement in the initial 3 months with DBS OFF. DBS implantation and stimulation of the CMN appears to be a safe and efficacious treatment, particularly in patients with refractory generalized epilepsy. CMN stimulation was not as effective in frontal lobe epilepsy, which requires further studies. DBS of the CMN should be considered as a treatment option, particularly in patients with refractory generalized epilepsy syndromes. | {
"pile_set_name": "PubMed Abstracts"
} |
Clinical characterisation of polydactyly in Maine Coon cats.
Objectives Polydactyly has been reported in a number of vertebrate species, including the domestic cat. It is a common characteristic in some breeding lines of the Maine Coon. The aim of this study was to assess the limb phenotype of polydactyl cats using physical and radiographic examinations. Methods We used physical examination and radiography to characterise the polydactyly phenotype in a cohort of 70 Maine Coon cats, including 48 polydactyl cats from four different breeding lines from Europe, Canada and the USA. Results The phenotypic expression of polydactyly showed great variability, not only in digit number and conformation, but also in the structure of the carpus and tarsus. Comparison of the size of the radius in polydactyl and non-polydactyl 3-month-old kittens and adult females did not reveal any difference between polydactyl and non-polydactyl cats. Conclusions and relevance We conclude that polydactyly in Maine Coon cats is characterised by broad phenotypic diversity. Polydactyly not only affects digit number and conformation, but also carpus and tarsus conformation, with no apparent deleterious consequence on feline welfare. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of dotarizine on peripheral and pulmonary circulation and cardiac dynamics in dogs.
The cardiovascular effects of dotarizine in 10-min intravenous infusions were studied in thiopental-anesthetized dogs. The effects of dotarizine 0.024 mg/kg/min almost paralleled those of saline controls; 0.079 mg/kg/min dotarizine significantly raised the stroke index and ejection fraction, and, at a rate of 0.25 mg/kg/min, further effects appeared and were dose-dependent. Dotarizine produced arterial dilation in both systemic and pulmonary circulation: the total peripheral resistance dropped, and femoral artery flow rose; aortic and pulmonary artery mean and diastolic pressures declined, and systolic pressures remained almost stable. A trend of bradycardia and pulmonary artery pressure reduction persisted for 30 min. As compared with the reduced total peripheral resistance, aortic pressure fell only moderately because of rising cardiac output due to a higher ejection fraction and stroke volume. Cardiac preload tended to decline; contractility tended to increase. Cardiac performance remained stable while myocardial oxygen consumption tended to fall, as did the pressure-rate product and the tension time index. Dotarizine exerted direct cardiovascular effects similar to those of the 5-HT2-receptor antagonist ketanserin and, more generally, to calcium channel blockers rather than to alpha-adrenoceptor blockers. | {
"pile_set_name": "PubMed Abstracts"
} |
Muscle RING-finger protein-1 (MuRF1) functions and cellular localization are regulated by SUMO1 post-translational modification.
The muscle RING-finger protein-1 (MuRF1) is an E3 ubiquitin ligase expressed in skeletal and cardiac muscle tissues and it plays important roles in muscle remodeling. Upregulation of MuRF1 gene transcription participates in skeletal muscle atrophy, on contrary downregulation of protein expression leads to cardiac hypertrophy. MuRF1 gene point mutations have been found to generate protein aggregate myopathies defined as muscle disorder characterized by protein accumulation in muscle fibers. We have discovered that MuRF1 turned out to be also a target for a new post-translational modification arbitrated by conjugation of SUMO1 and it is mediated by the SUMO ligases E2 UBC9 and the E3 PIASγ/4. SUMOylation takes place at lysine 238 localized at the second coiled-coil protein domain that is required for efficient substrate interaction for polyubiquitination. We provided evidence that SUMOylation is essential for MuRF1 nuclear translocation and its mitochondria accumulation is enhanced in hyperglycemic conditions delivering a stabilization of the overall SUMOylated proteins in cultured myocytes. Thus, our findings add this SUMO1 post-translational modification as a new concept to understand muscle disorders related to the defect in MuRF1 activity. | {
"pile_set_name": "PubMed Abstracts"
} |
Transverse relaxation in the rotating frame induced by chemical exchange.
In the presence of radiofrequency irradiation, relaxation of magnetization aligned with the effective magnetic field is characterized by the time constant T1rho. On the other hand, the time constant T2rho characterizes the relaxation of magnetization that is perpendicular to the effective field. Here, it is shown that T2rho can be measured directly with Carr-Purcell sequences composed of a train of adiabatic full-passage (AFP) pulses. During adiabatic rotation, T2rho characterizes the relaxation of the magnetization, which under adiabatic conditions remains approximately perpendicular to the time-dependent effective field. Theory is derived to describe the influence of chemical exchange on T2rho relaxation in the fast-exchange regime, with time constant defined as T2rho,ex. The derived theory predicts the rate constant R2rho,ex (= 1/T2rho,ex) to be dependent on the choice of amplitude- and frequency-modulation functions used in the AFP pulses. Measurements of R2rho,ex of the water/ethanol exchanging system confirm the predicted dependence on modulation functions. The described theoretical framework and adiabatic methods represent new tools to probe exchanging systems. | {
"pile_set_name": "PubMed Abstracts"
} |
The aggressive surgical management of hypercalcemia due to ectopic parathormone production.
A patient with slowly progressive metastatic renal cell carcinoma had life-threatening hypercalcemia secondary to ectopic parathormone production. Aggressive surgical debulking resulted in prolonged control of the hypercalcemia. This case illustrates the value of this surgery in the control of paraneoplastic endocrine syndromes refractory to medical management in patients with slow-growing metastatic disease. | {
"pile_set_name": "PubMed Abstracts"
} |
Feasibility of estimating regional mechanical properties of cerebral aneurysms in vivo.
In this article, the authors studied the feasibility of estimating regional mechanical properties in cerebral aneurysms, integrating information extracted from imaging and physiological data with generic computational models of the arterial wall behavior. A data assimilation framework was developed to incorporate patient-specific geometries into a given biomechanical model, whereas wall motion estimates were obtained from applying registration techniques to a pair of simulated MR images and guided the mechanical parameter estimation. A simple incompressible linear and isotropic Hookean model coupled with computational fluid-dynamics was employed as a first approximation for computational purposes. Additionally, an automatic clustering technique was developed to reduce the number of parameters to assimilate at the optimization stage and it considerably accelerated the convergence of the simulations. Several in silico experiments were designed to assess the influence of aneurysm geometrical characteristics and the accuracy of wall motion estimates on the mechanical property estimates. Hence, the proposed methodology was applied to six real cerebral aneurysms and tested against a varying number of regions with different elasticity, different mesh discretization, imaging resolution, and registration configurations. Several in silico experiments were conducted to investigate the feasibility of the proposed workflow, results found suggesting that the estimation of the mechanical properties was mainly influenced by the image spatial resolution and the chosen registration configuration. According to the in silico experiments, the minimal spatial resolution needed to extract wall pulsation measurements with enough accuracy to guide the proposed data assimilation framework was of 0.1 mm. Current routine imaging modalities do not have such a high spatial resolution and therefore the proposed data assimilation framework cannot currently be used on in vivo data to reliably estimate regional properties in cerebral aneurysms. Besides, it was observed that the incorporation of fluid-structure interaction in a biomechanical model with linear and isotropic material properties did not have a substantial influence in the final results. | {
"pile_set_name": "PubMed Abstracts"
} |
Stress Resilience Measurement With Heart-Rate Variability During Mental And Physical Stress.
Stress management is particularly important for healthcare of modern people. In stress research, heart-rate variability (HRV), indicating the change of time intervals in successive heart beats, significantly contributed due to its close relationship with autonomic nervous system. However, the adaptive response to stress, also known as stress resilience, has not been studied much yet. We collected electrocardiogram during mental and physical stress, experimentally designed by mental arithmetic tasks and physical activities for 14 healthy subjects. As a result, we found that resting HRV parameters, particularly associated with the parasympathetic activity, had significant positive correlations with reactivity and recovery from mental and physical stress. These HRV parameters can be used as a measure of stress resilience quantitatively. Our findings suggest that these parameters can help one's stress management by enabling to predict the adaptive response to upcoming stressful events. | {
"pile_set_name": "PubMed Abstracts"
} |
Inhibition of glutathione synthesis eliminates the adaptive response of ascitic hepatoma 22 cells to nedaplatin that targets thioredoxin reductase.
Thioredoxin reductase (TrxR) is a target for cancer therapy and the anticancer mechanism of cisplatin involves TrxR inhibition. We hypothesize that the anticancer drug nedaplatin (NDP), an analogue of cisplatin and a second-generation platinum complex, also targets TrxR. Furthermore, we investigate whether the therapeutic efficacy of NDP can be enhanced by simultaneous modulation of 1) TrxR, via NDP, and 2) glutathione (GSH), via the GSH synthesis inhibitor buthionine sulfoximine (BSO). Mice bearing ascitic hepatoma 22 (H22) cells were treated with NDP alone or NDP plus BSO. TrxR activity of H22 cells was inhibited by NDP in a dose-dependent manner. A high correlation between the inhibition of TrxR activity at 6h and the inhibition of ascitic fluid volume at 72h was established (r=0.978, p<0.01). As an adaptive response, the viable ascitic cancer cells after NDP treatment displayed an enlarged cell phenotype, assembled with several-fold more antioxidant enzymes and GSH-predominant non-protein free thiols. This adaptive response was largely eliminated when BSO was co-administered with NDP, leading to the decimation of the H22 cell population without enhancing renal toxicity, since at this dose, NDP did not inhibit renal TrxR activity. In conclusion, the pharmacological effect of NDP involves TrxR inhibition, and the adaptive response of NDP-treated ascitic H22 cells can be efficiently counteracted by BSO. Simultaneous modulation of TrxR and GSH on ascitic H22 cells using NDP plus BSO greatly enhances therapeutic efficacy as compared with the single modulation of TrxR using NDP alone. | {
"pile_set_name": "PubMed Abstracts"
} |
[Diagnostic value of spinal puncture-biopsy in non-infectious spinal diseases. Apropos of 41 cases].
To evaluate the diagnostic value of percutaneous vertebral biopsy in noninfectious diseases of the spine, we retrospectively studied 41 cases seen between 1985 and 1992. The level of the lesion was lumbar in 29 cases, thoracic in 11, and cervical in one. There were 19 crush fractures, 11 lytic lesions, six sclerotic lesions, and three mixed lesions. The biopsy was done because of an abnormal magnetic resonance imaging signal in one patient and because of epiduritis in another. The thoracic and lumbar biopsies were done under x-ray guidance using the technique developed by Laredo and Bard. Computed tomography guidance was used for the cervical biopsy. There were no adverse events. The final histological diagnosis was metastatic disease in 17 cases (41.5%), myeloma or plasmacytoma in six cases (14.7%), primary vertebral neoplasia in two cases (4.8%), lymphoma in one case (2.4%), osteoporosis in nine cases (22%), Paget's disease in three cases (7.4%), amyloidosis in one case (2.4%), aseptic osteitis in one case (2.4%), and vertebral necrosis in one case (2.4%). A second biopsy procedure was done in three patients (surgically in two cases and percutaneously in one) because of discrepancies between histological findings and other data. The final diagnosis was metastatic disease in all three patients. Overall, the diagnostic yield of percutaneous vertebral biopsy was 92.6% and varied little with initial roentgenographic or computed tomographic findings. However, yield was only 56% for the diagnosis of tumorous lesions, with variations according to roentgenographic and computed tomographic changes, 90.1% for osteolytic lesions, 66.6% for mixed lesions, 47.4% for crush fractures, and 16.6% for sclerotic lesions.(ABSTRACT TRUNCATED AT 250 WORDS) | {
"pile_set_name": "PubMed Abstracts"
} |
Pheromones among the procaryotes.
A pheromone is a chemical excreted by an organism into the environment that acts to elicit a specific response from other organisms of the same species. The importance of pheromones in the life cycle of various species of mammals, insects, and fungi is well known. In the past decade, it has become apparent that pheromones influence the behavior and development of prokaryotes. Pheromones excreted by myxobacteria, actinomycetes, and cyanobacteria elicit specific developmental responses from these organisms. In addition, pheromones excreted by Streptococcus faecalis function in conjugation, and pheromones of luminescent bacteria regulate bioluminescence of these organisms. | {
"pile_set_name": "PubMed Abstracts"
} |
[Type III osteogenesis imperfecta associated with hypophosphatemic vitamin D-resistant rickets].
In a 7 year old girl presenting with bone deformities, dwarfism, and a history of recurrent fractures osteogenesis imperfecta had been diagnosed at birth. Although she had been hospitalized several times, radiologic signs of rickets remained unnoticed. Laboratory data proved existence of hypophosphatemic vitamin D-resistant type of rickets, which was effectively treated with 1 alpha-hydroxycholecalciferol and phosphorus substitution. The combination of osteogenesis imperfecta type III and hypophosphatemic rickets may be coincident. It proves, however, the necessity to consider the possible simultaneous occurrence of two rare diseases. The therapeutic consequences could be important. | {
"pile_set_name": "PubMed Abstracts"
} |
[Lead elimination by traditional curing].
To evaluate traditional acidic curing as a preventive method for reducing lead content of glazed ceramic ware. In 27 ceramic ware pieces from four states in Mexico, the level of residual lead was determined through atomic absorption spectrophotometry after four washings with acetic acid 3%. The lead content of the vinegar washing diminished proportionally with the number of washings, although it remained highly above the permissible levels of this metal (2.5-7.0 p.p.m.). Traditional acidic curing of glazed ceramic ware is not a useful preventive measure for reducing lead exposure. | {
"pile_set_name": "PubMed Abstracts"
} |
High-performance liquid chromatography with electrochemical detection applied to the analysis of 3,4-dihydroxymethamphetamine in human plasma and urine.
Metabolic activation in the disposition of 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy") has been implicated in some of its pharmacological and toxicological effects, with the major metabolite 3,4-dihydroxymethamphetamine (HHMA) as a putative toxicant through the formation of thioether adducts. We describe the first validated method for HHMA determination based on acid hydrolysis of plasma and urine samples, further extraction by a solid-phase strong cation-exchange resin (SCX, benzenesulfonic acid), and analysis of extracts by high-performance liquid chromatography with electrochemical detection. The chromatographic separation was performed in an n-butyl-silane (C4) column and the mobile phase was a mixture of 0.1 M sodium acetate containing 0.1 M 1-octanesulphonic acid and 4 mM EDTA (pH 3.1) and acetonitrile (82:18, v/v). Compounds were monitored with an electrochemical cell (working potentials 1 and 2, +0.05 and +0.35 V, respectively, gain 60 microA). A mobile phase conditioning cell with a potential set at +0.40 V was connected between the pumping system and the injector. Calibration curves were linear within the working concentration ranges of 50-1000 microg/L for urine and plasma. Limits of detection and quantification were 10.5 and 31.8 microg/L for urine and 9.2 and 28.2 microg/L for plasma. Recoveries for HHMA and DHBA (3,4-dihydroxybenzylamine, internal standard) were close to 50% for both biological matrices. Intermediate precision and inter-day accuracy were within 3.9-6.5% and 7.4-15.3% for urine and 5.0-10.8% and 9.2-13.4% for plasma. | {
"pile_set_name": "PubMed Abstracts"
} |
VUV/Vis Photoluminescence, Site Occupancy, and Thermal-Resistance Properties of K4 SrSi3 O9 :Ce3.
A series of Ce3+ -activated K4 SrSi3 O9 materials emitting bright blue light under near-UV excitation was obtained by a facile solid-state reaction. The luminescence of Ce3+ -doped K4 SrSi3 O9 shows excellent thermal stability in a large temperature range. The detailed temperature/concentration-dependent luminescent properties of Ce3+ confirm that there are two different Ce3+ sites in K4 SrSi3 O9 :Ce3+ , and energy transfer from the high-energy Ce(1)3+ sites to the low-energy Ce(2)3+ sites was demonstrated to occur by electric dipole-dipole interaction. The maximum photoluminescence quantum yield of Ce3+ -doped K4 SrSi3 O9 can reach 56 %, and a UV-excited white LED device with low correlated color temperature (CCT=4617 K) and high color-rendering index (Ra =80) was fabricated with K4 SrSi3 O9 :Ce3+ . | {
"pile_set_name": "PubMed Abstracts"
} |
[Treatment of hypermetropia using the Holmium laser--laser thermokeratoplasty (LTK)].
The results of two year follow-up after Holmium LTK are presented. The choice of the technique was justified based on experimental, technical and anatomo-pathological data as well as on the property of the corneal collagen fibers to shrink at a temperature of 60-70 degrees C. Although the immediate results were encouraging, we found an important regression after two years follow-up, resulting in a final correction of maximally 1.5 D, independent on the degree of hypermetropia to be treated. These results were obtained with the "contact" method, which is in our experience slightly superior to the "non-contact" technique. The question is whether the regression will continue with time or will stabilize and remain at 1.5 D of hyperopic correction. | {
"pile_set_name": "PubMed Abstracts"
} |
Combination of 4-epi-doxorubicin and irradiation--a new approach in the treatment of locoregionally advanced inoperable esophageal cancer.
The synergistic activity observed in vitro in V-79 hamster lung cells after treatment with 4-epi-doxorubicin (4-epi-DX) combined with irradiation stimulated a pilot study of 38 patients with inoperable locoregionally advanced squamous cell esophageal cancer. The patients (30 males, 8 females; mean age 60 years) had undergone no prior radiation or cytostatic drug therapy. Twenty tumors were localized in the middle third of the esophagus, and the remainder in the upper or lower third. Histological evidence of the tumor was obtained in all patients before treatment by endoscopy. The 33 evaluable cases included 30 squamous cell carcinomas, 2 anaplastic (squamous cell) carcinomas and 1 adenocarcinoma. The patients were irradiated in two opposite thoracic fields (Betatron-Siemens) with a total dosage of 3600-4000 cGy (200 cGy daily, 1000 weekly). The patients were concurrently administered 4-epi-DX at the dose of 50 mg/m2 i.v. daily on days 1, 2, 22 and 23; the total 4-epi-DX dosage was 200 mg/m2. The results showed that 4-epi-DX combined with irradiation had a pronounced antitumorigenic activity, since the 33 evaluable patients included 11 complete and 12 partial remissions, with a response rate of 70% (23/33). A minor regression (less than 50%) was observed in 6 cases, and progression of the disease in 4. The median duration of the remissions was 9 + months (14 + months in complete responders). In 8 patients with a complete clinical response even the endoscopic biopsy samples were negative. Toxicity was moderate and reversible, and mainly accounted for by radiation mucositis and retrosternal pain, alopecia and mild bone marrow suppression. Transient ECG changes were observed in 3 cases. The results of the pilot study show that the combination of 4-epi-DX and irradiation might constitute successful palliative treatment for squamous cell esophageal cancer. | {
"pile_set_name": "PubMed Abstracts"
} |
Chromium(0), Molybdenum(0), and Tungsten(0) Isocyanide Complexes as Luminophores and Photosensitizers with Long-Lived Excited States.
Arylisocyanide complexes based on earth-abundant Group 6 d6 metals are interesting alternatives to photoactive complexes made from precious metals such as RuII , ReI , OsII , or IrIII . Some of these complexes have long-lived 3 MLCT excited states that exhibit luminescence with good quantum yields as well as nano- to microsecond lifetimes, and they are very strongly reducing. Recent studies have demonstrated that Cr0 , Mo0 , and W0 arylisocyanide complexes have great potential for applications in luminescent devices, photoredox catalysis, and dye-sensitized solar cells. | {
"pile_set_name": "PubMed Abstracts"
} |
Inclusivity in neo-liberal policy environment: preparedness of mass media to support disability awareness campaigns in Ghana.
The mass media have been described as an important institution in educating society about persons with disabilities and in facilitating their acceptance. However, calls to project a positive public image of persons with disabilities and to develop an inclusive society have coincided with the neo-liberal approach to policymaking, whereby government plays a minimised role in the provision of basic services to citizens. Although the extant literature from advanced countries has found stereotypical and disempowering portrayals of persons with disabilities in the mass media, this area remains unresearched in developing countries. The purpose of this study was to explore how the managers of media organisations conceptualise disability, their perception of the role and contribution of the media towards promoting effective public awareness campaigns about persons with disabilities. This descriptive, qualitative study draws on a purposive sample of 20 participants consisting of general managers, news editors and programme managers from ten media organisations in a region in Ghana. A semi-structured interview guide was developed from the literature, which included tenets of neo-liberalism, to collect the data. The study found that the majority of respondents cited medical model explanations of disability. Also, they were unprepared to prioritise disability issues due to lack of public interest in disability programmes and lack of funds and sponsorship for disability programmes. Governments must offer tax rebates to media organisations to enable them to prioritise disability issues. Other recommendations for developing media interest in disability issues are also discussed. Implications for rehabilitation This study shows limited understanding of disability among decision-makers or key players in media organisations in Ghana. In an environment of meritocracy, persons with disabilities who struggle to access education in Ghana, may be unable to secure jobs at media organisation. This study shows that media organisations seem unprepared to support or project image of persons with disabilities because such ventures come with no reward. We conclude that if the government is committed towards achieving an inclusive society in Ghana, it could offer tax rebate to media organisations to enable them cover disability programmes. | {
"pile_set_name": "PubMed Abstracts"
} |
Biochemical changes in articular cartilage opposing full- and partial-thickness cartilage lesions in horses.
Using arthroscopic technique, identical diameter defects were created in the proximal articular surface of both intermediate carpal bones of 6 horses. One of each pair of defects was deepened to penetrate the subchondral plate. Removed cartilage was assayed for [35S] sulfate incorporation, total hexosamine content, and DNA content. Six weeks later, cartilage was harvested and similarly analyzed from the distolateral portion of the radius directly opposite the created lesions and the distomedial portion of the radius distant from the lesion. The repair tissue filling the full-thickness defect and the cartilage at the periphery of the partial-thickness lesion also were analyzed. There was a marked increase in synthetic activity (35S sulfate incorporation) opposite the full-thickness defect, compared with the cartilage opposite the partial-thickness defect. A marked decrease in glycosaminoglycan content in the cartilage opposite the full-thickness defect was found as compared with that opposite the partial-thickness defect. The repair tissue filling the full-thickness defect was highly cellular, high in synthetic activity, but low in glycosaminoglycan content. Insignificant changes occurred in the cartilage adjacent to the partial-thickness defect. On the basis of these results, we suggest that full-thickness defects at 6 weeks result in more detrimental change to the cartilage opposite it than do partial-thickness lesions of the same diameter. | {
"pile_set_name": "PubMed Abstracts"
} |
rFVIIa administered by continuous infusion during surgery in patients with severe congenital FVII deficiency.
The use of recombinant FVIIa (rFVIIa) to control bleed in individuals with FVII deficiency has been proven to be effective. The main problems associated with its use are that it requires frequent bolus injections to counteract its short half-life and high cost. Our study aimed to evaluate whether any advantage could be gained by providing rFVIIa by continuous infusion during surgery with regard to haemostatic efficacy, safety and cost. The prospective study included 10 patients with severe FVII deficiency, who underwent 25 surgical procedures (13 major and 12 minor procedures) and were treated with rFVIIa administered by continuous infusion. Tranexamic acid was given concomitantly every 8 h. Prothrombin time, FVII:C assay and thrombin generation assay were used to monitor the treatment. The mean total dose given was 10 mg during a major surgery and 4.4 mg during a minor surgery for a mean treatment duration of 7.5 and 4.0 days respectively. This corresponds to a reduction of 70-90% in drug usage and medication cost compared with bolus injections. Except for one major perioperative bleeding, excellent haemostasis was achieved in all procedures. One patient developed a transient inhibitory activity. None of these events affected the postoperative course or prolonged the hospital stay. Our study demonstrated that continuous infusion of rFVIIa during surgery is safe, effective and highly cost effective. | {
"pile_set_name": "PubMed Abstracts"
} |
Information exchange yields better decisions.
A new study from the Medical College of Wisconsin shows that the Wisconsin Health Information Exchange has not only saved time for the EDs participating, but has helped them make better-informed patient care decisions. EDs in the Milwaukee area are linked to the system with a dedicated computer housed in a separate area of the department. Once registration enters the patient's name into the system, basic information appears about the previous ED visits at all facilities in the city. ED staff can also obtain additional patient information if the need arises. | {
"pile_set_name": "PubMed Abstracts"
} |
[Effect of neostigmine on atracurium-induced neuromuscular blockage in patients pretreated with magnesium sulphate].
To determine the effect of neostigmine on antagonizing atracurium-induced neuromuscular blockage with sulfate magnesium pretreatment. Forty patients who undertook elective gynecologic laparoscopic examinations and treatments under general anesthesia were randomized into four groups (group A, B, C, and D, group A paired with group C, and group B paired with group D). Before induction of general anesthesia, patients in group A and group C received MgSO4 30 mg/kg in saline intravenously within 5 min, while patients in group B and group D received the same volume of saline. Anesthesia was induced with fentanyl and propofol; subsequently tracheal intubation was performed with 0.5 mg/kg atracurium after stabilization of the electromyography recording, and neostigmine (0.02 mg/kg) and atropine (0.01 mg/kg) were infused in group C and group D when neuromuscular recovery (T1/T(C)) reached 10%. T1/T(C) changes after neostigmine infusion as well as haemodynamic changes and other responses during induction and neostigmine and atropine infusion were recorded. The neuromuscular recovery speed had no significant difference between group A and group B after the neuromuscular recovery reached 10%, but it was lower in group C than in group D (P < 0.05). Significant difference existed between group AC and group BD (P < 0.05). No haemodynamic changes and other responses were found during induction and neostigmine and atropine infusion. Neostigmine-induced neuromuscular recovery can be attenuated in patients pretreated with magnesium sulfate. | {
"pile_set_name": "PubMed Abstracts"
} |
External beam radiation therapy in the treatment of patients with extranodal stage IA non-Hodgkin's lymphoma.
The purpose of this report was to study the results of external beam radiotherapy for patients with extranodal stage IA non-Hodgkin's lymphoma (NHL). A retrospective review was carried out on 27 patients seen between 1984 and 1998 with stage IA NHL of extranodal sites, and followed up for a minimum of 1 year. Sites involved included eye/orbit (seven), parotid (five), breast (four), Waldeyer ring (four), thyroid (three), other head and neck (two), stomach (one), and prostate (one). All patients had biopsy-proven disease and underwent staging workup to rule out other sites of disease. Histologic analysis revealed 16 patients with low-grade NHL, 9 with intermediate-grade, and 2 with high-grade. Ten patients received chemotherapy before radiation therapy, and eight of them had a complete response. The remaining 17 patients were treated with external beam radiation therapy alone. Radiation was directed to the involved field at 1.8 Gy to 2.0 Gy per fraction to a median dose of 40 Gy (range: 20-50.4 Gy). The median patient age was 71 years (range: 39-85 years); 55% were female, and 45% were male. A complete response was attained in all 27 patients after radiation therapy. There were five failures (all in uninvolved distant sites), and two deaths during the follow-up. Median disease free survival (DFS) and overall survival (OS) have not been reached. The 5-year DFS and OS are 85% and 94%, respectively. Older age at presentation showed a trend toward worse outcome (p = 0.07), but because of the relatively few events, other factors (radiation dose, grade of disease, sex, or the use of chemotherapy) showed no statistical differences among the patients. External beam radiation therapy is a highly effective treatment for stage IA NHL found in extranodal sites. | {
"pile_set_name": "PubMed Abstracts"
} |
PGR and PTX3 gene expression in cumulus cells from obese and normal weighting women after administration of long-acting recombinant follicle-stimulating hormone for controlled ovarian stimulation.
The present study aimed to determine clinical IVF parameters and gene expression in cumulus cells (CCs) in obese and normal weighting women after administration of 150 mcg of corifollitropin alfa for controlled ovarian hyperstimulation (COH). 150 mcg of corifollitropin alfa and gonadotropin releasing hormone antagonist were used for COH. Analysis of CC gene expression was performed using quantitative real-time PCR. We did not find significant differences in biochemical and clinical pregnancy rates between obese and normal weighting women. Obese women required twice as much of additional gonadotropins for ovarian stimulation and had a significantly lower proportion of good quality embryos on day 5 of IVF cycle. Expression of PGR and PTX3 was significantly higher in CCs of obese women. Obese women require significantly larger amounts of gonadotropins to achieve similar IVF success rates as normal weighting women. Differences in CC gene expression and smaller proportion of good quality embryos may imply that oocytes derived from obese women are of lower quality. Further studies are needed to evaluate whether obesity itself or the higher amount of gonadotropins used in obese women causes this effect. | {
"pile_set_name": "PubMed Abstracts"
} |
Serotonin axon terminals in the ventral tegmental area of the rat: fine structure and synaptic input to dopaminergic neurons.
The serotoninergic (5-hydroxytryptamine, 5-HT) innervation of the rat ventral tegmental area (VTA) was examined by light and electron microscopic radioautography following intraventricular infusion of [3H]5-HT. The [3H]5-HT labeled processes were characterized with respect to their regional distribution, ultrastructure and relationships with all neurons, including dopaminergic neurons, identified in the same sections using immunocytochemistry for the localization of the catecholamine-synthesizing enzyme, tyrosine hydroxylase (TH). By light microscopy, [3H]5-HT labeled axons and axonal varicosities were detected throughout the interfascicular nucleus and ventral portion of the VTA. By electron microscopy, [3H]5-HT-labeled axons were found to be mainly small and unmyelinated, although a few showed several lamellae of myelin. The labeled varicosities measured 0.6 micron in mean diameter and contained many small, round or flattened agranular vesicles and a few large granular vesicles. More than 18% showed synaptic specializations in single thin sections. Most of these synapses were asymmetric and established on dendritic shafts. Based on the probability of seeing such synaptic specializations in single thin sections, it was estimated that as many as 50% of the labeled 5-HT terminals formed synaptic contacts in the VTA. In dually labeled light microscopic sections, [3H]5-HT-accumulating processes often appeared adjacent to TH-immunoreactive perikarya and proximal dendrites. Electron microscopy demonstrated that terminals with radioautographic labeling for 5-HT formed conventional synapses both with TH-labeled and unlabeled dendrites in the VTA. Many additional 5-HT terminals lacking recognizable synaptic densities were directly apposed to TH-labeled dendrites and were isolated from the rest of the neuropil by thin glial leaflets. These results suggest that 5-HT neurons innervate both dopaminergic and non-dopaminergic neurons in the VTA and may influence mesocortical and mesolimbic efferent systems through synaptic as well as non-synaptic mechanisms. | {
"pile_set_name": "PubMed Abstracts"
} |
Distribution of organochlorine pesticides in an agricultural environment, Holland Marsh, Ontario--1970-72.
Analaysis of organochlorine pesticides in soil, fish, and human blood samples from Holland Marsh, Ontario, indicates that although total DDT is present in detectable amounts, it does not constitute a hazard to human health and longevity. Among soils tested, residues were highest in surface samples. DDT levels in human blood samples were similar to those in U.S. and British studies. | {
"pile_set_name": "PubMed Abstracts"
} |
Evaluation of sequential presence-absence sampling plans for the diamondback moth (Plutellidae: Lepidoptera) in cruciferous crops in Australia.
Two sets of sequential presence-absence sampling plans for decision-making in the management of diamondback moth, Plutella xylostella (L.), were developed and evaluated. One set of sampling plans targeted the classification of proportions of infested plants, and the other set of sampling plans targeted the classification of larval density. The action thresholds investigated were 0.15, 0.25, 0.35, and 0.45 proportion of plants infested with larvae, and 0.2, 0.4, 0.6, and 0.8 larvae per plant. They are representative of the action thresholds currently practiced by Australian crucifer growers. For each sampling plan, the population range within which a minimal correct decision rate of 95% can be expected at a maximal average sample size of 50 plants (OC95ASN50) was specified. The closeness of an OC95ASN50 range to the target action threshold is a measure of the expected performance of the sampling plan. A closer distance reflects better performance. The OC95ASN50 ranges of the proportion-classification sampling plans were within 33-53% of the target action thresholds. The width of these OC95ASN50 ranges represents 73-87% of the entire population range (0-1). For the classification of larval density, an empirical proportion-density model was first established using data from different states and different cruciferous crops. The OC95ASN50 ranges of the density-classification sampling plans were within 57-75% of the target action threshold. Simulated sampling of 20 independent data sets showed that for most data sets the correct classification rate was at least 98% and the matching average sample size was <50 plants. | {
"pile_set_name": "PubMed Abstracts"
} |
Refractive outcomes and quality of vision related to an outbreak of diffuse lamellar keratitis.
To report the impact on visual function and corneal aberrations during an epidemic of diffuse lamellar keratitis (DLK). A retrospective, comparative, and consecutive case-control study was performed, involving 418 eyes operated between March and October 2007 in the refractive surgery unit of one center. Eyes were separated into groups: group 1 (DLK group) included 209 eyes that presented with DLK after LASIK and group 2 (control group) included 209 unaffected, consecutive eyes operated from the beginning of the outbreak. Refractive variables and corneal aberrations were compared between the two groups. Visual outcomes were significantly different when comparing control eyes and eyes with DLK grades 3 and 4, but not for eyes with DLK grades 1 and 2. Uncorrected distance visual acuity (UDVA) significantly improved from the third month after DLK was diagnosed (Kruskal-Wallis, P=.02). Corrected distance visual acuity (CDVA) did not decrease significantly with DLK grades 1 and 2 (preoperative CDVA vs 1, 3, and 6 months after diagnosis; Mann-Whitney, P>.05, respectively). At the end of follow-up, UDVA and CDVA were not significantly different between the two groups. Eyes suffering from DLK had significantly increased corneal aberrations, except for spherical and astigmatism aberrations, 3 months after being diagnosed (Kruskal-Wallis, P<.05); however, corneal aberrations were not significantly different between the DLK and control groups (Mann-Whitney, P>.05). The outbreak of DLK did not induce a significant decrease in global visual outcomes, except in severe cases, and postoperative corneal aberrations were not affected. | {
"pile_set_name": "PubMed Abstracts"
} |
Guilty by mere association: evaluative conditioning and the spreading attitude effect.
Five experiments investigated the phenomenon that attitude formation is not confined to the co-occurrence of an attitudinal object with an evaluated experience. The pairing of a target with a (dis)liked person not only affects the evaluation of the previously neutral person but spreads to other individuals who are (pre)associated with the target (spreading attitude effect). Experiments 1 and 2 provided evidence for the spreading attitude effect in appetitive as well as aversive evaluative conditioning. Experiment 3 showed that the spreading attitude effect is a robust phenomenon resistant to extinction. Experiment 4 demonstrated that attitude spread can be transferred to 2nd-order conditioning. Finally, Experiment 5 supports the notion that the spreading attitude effect is not dependent on cognitive resources. Implications for social as well as applied psychology are discussed. | {
"pile_set_name": "PubMed Abstracts"
} |
Depth of invasion as a predictive factor for cervical lymph node metastasis in tongue carcinoma.
Many histopathologic parameters in head and neck squamous cell carcinoma have been identified as predictive factors for cervical metastasis. Several studies focused on tumor thickness, and the depth of invasion was suggested to have a relationship to the occurrence of cervical metastasis. Nonetheless, the criterium for elective neck therapy in terms of tumor depth is still inconclusive. Therefore, a retrospective study was undertaken to substantiate the differing results in the literature with our own findings concerning the interrelationship between tumor thickness and clinically suspicious neck, as well as occult neck disease. In addition, the study attempted to identify further predictive factors for cervical metastasis in squamous cell carcinoma of the tongue. The medical records of 34 primary tongue carcinomas operated without any preoperative therapy between 1980 and 1991 were reviewed. Each patient's tumor type, tumor location, tumor size, invasion mode, depth of invasion, intralymphatic tumor emboli, and perineural invasion were evaluated. Chi-square contingency tables were used to correlate clinical or histopathologic parameters with metastasis in the neck. The overall cervical metastatic rate was 35.3% (n = 12/34). In univariate analysis, invasion mode and depth of invasion were statistically significant predictors of regional metastasis at p = 0.0019 and p = 0.0003, respectively. In the group in which tumor depth exceeded 5 mm, the metastatic rate was 64.7% (11/17). In contrast, when the depth of invasion was less than 5 mm, the incidence of cervical metastasis was 5.9% (1/17). Clinically negative necks turned out pathologically positive in 30.0% (n = 9/30). The tumor depth exceeded 5 mm in 43.4% (13/30) of the N0 necks. In the conversion (N0-->N1) group, tumor depth exceeding 5 mm was noted in 88.9% (n = 8/9). We suggest that there is a discerning point at 5 mm of tumor depth at which cervical metastasis is probable. Electric neck therapy (surgery or irradiation) is strongly indicated for tumors exceeding 5 mm invasion. Tumor invasion mode and tumor size also bear an impact on the indication for therapy. | {
"pile_set_name": "PubMed Abstracts"
} |
Fast recursive filters for simulating nonlinear dynamic systems.
A fast and accurate computational scheme for simulating nonlinear dynamic systems is presented. The scheme assumes that the system can be represented by a combination of components of only two different types: first-order low-pass filters and static nonlinearities. The parameters of these filters and nonlinearities may depend on system variables, and the topology of the system may be complex, including feedback. Several examples taken from neuroscience are given: phototransduction, photopigment bleaching, and spike generation according to the Hodgkin-Huxley equations. The scheme uses two slightly different forms of autoregressive filters, with an implicit delay of zero for feedforward control and an implicit delay of half a sample distance for feedback control. On a fairly complex model of the macaque retinal horizontal cell, it computes, for a given level of accuracy, one to two orders of magnitude faster than the fourth-order Runge-Kutta. The computational scheme has minimal memory requirements and is also suited for computation on a stream processor, such as a graphical processing unit. | {
"pile_set_name": "PubMed Abstracts"
} |
Treatment of local-anesthetic toxicity with lipid emulsion therapy.
The use of lipid emulsion to treat local-anesthetic toxicity is discussed. Systemic toxicity from local anesthetics is a rare but potentially fatal complication of regional anesthesia. There is increasing evidence that lipid emulsion may be an effective treatment to reverse the cardiac and neurologic effects of local-anesthetic toxicity. A literature search identified seven case reports of local-anesthetic toxicity in which lipid emulsion was used. Lipid emulsion was found to be successful in the treatment of local-anesthetic toxicity associated with various regional anesthetic techniques and multiple local anesthetics. The majority of patients in the case reports reviewed were unresponsive to initial management of local-anesthetic toxicity with standard resuscitative measures, but all recovered completely after receiving lipid emulsion therapy. The initial dose of lipid emulsion administered varied among the case reports, as well as whether a lipid emulsion infusion was started and at what point during resuscitation. Based on the case reports reviewed, an initial bolus dose of 1.5 mL/kg followed by an infusion of 10 mL/min as soon as local-anesthetic toxicity is suspected seems most beneficial. The pharmacokinetics of lipid emulsion therapy in the treatment of local-anesthetic toxicity has not been fully elucidated but likely involves increasing metabolism, distribution, or partitioning of the local anesthetic away from receptors into lipid within tissues. Lipid emulsion has been reported useful in the treatment of systemic toxicity caused by local anesthetics. The mechanism of effect is unclear, and evidence for the benefit of lipid therapy in humans is from case reports only. | {
"pile_set_name": "PubMed Abstracts"
} |
Estrogen and progesterone receptors expression in gastrointestinal stromal tumors and intramural gastrointestinal leiomyomas.
Gastrointestinal stromal tumors (GISTs) and leiomyomas of the gastrointestinal tract both may present as intramural smooth muscle cell tumors. Immunohistochemically, these tumors demonstrate different spectrums of protein expression, which helps in differential diagnosis. Leiomyomas of the uterine type are similar by the spectrum of protein expression to gastrointestinal leiomyomas, but they express in addition both estrogen (ER) and progesterone (PR) receptors, which makes hormonal therapy possible for these tumors. In the current study, we investigated expression of ER and PR in GISTs and intramural gastrointestinal leiomyomas. Our data demonstrate that none of the 22 cases of gastrointestinal leiomyomas nor 19 cases of GIST were positive for either ER or PR expression. In contrast, leiomyomas of the uterine type were strongly positive for both ER and PR. Our data demonstrate that GIST and gastrointestinal leiomyomas differ from leiomyomas of the uterine type by the expression of ER and PR. Immunostaining for ER and PR may be a useful tool to distinguish these tumors and more precisely to plan further clinical interventions. | {
"pile_set_name": "PubMed Abstracts"
} |
[Duodenal obstruction by Strongyloides stercoralis, an unusual complication].
Strongyloidiasis is a neglected disease in Latin America. Gastrointestinal manifestations are nonspecific and duodenal obstruction is a rare complication. Here we present the case of a 31-year-old male from the central jungle of Peru, admitted due to a high intestinal obstruction, with duodenal ulcers and stenosis evidenced in the upper endoscopy. The histopathological report revealed presence of larvae of Strongyloides stercoralis. Clinical and endoscopic follow up were favorable with ivermectin treatment. There are near 20 reported cases of duodenal obstruction due to S. stercoralis. Additionally, infection by HTLV-1 was confirmed, being this a frequent association. | {
"pile_set_name": "PubMed Abstracts"
} |
Pulmonary toxicity associated with pomalidomide.
Pomalidomide, previously used to treat multiple myeloma, has been reported to cause acute pulmonary toxicity that improves with drug discontinuation. We present a case of delayed pneumonitis with persistent fibrosis associated with pomalidomide. A 61-year-old male treated with pomalidomide and corticosteroids presented with acute on chronic dyspnea, profound hypoxemia, and ground glass opacities on computerized tomographic imaging. Corticosteroid taper and discontinuation of pomalidomide resulted in clinical improvement, but with substantial residual pulmonary fibrosis. Given the temporal improvement, but not resolution, following discontinuation of an agent with an established propensity for lung injury, we attribute this presentation to pomalidomide toxicity. | {
"pile_set_name": "PubMed Abstracts"
} |
[Validation of ultrasound diagnosis of fetal anomalies at a reference center in Pernambuco].
To validate ultrasound diagnoses of fetal anomalies made in a Fetal Medicine Center in Pernambuco. A cross sectional study was performed to validate the diagnosis test, including all high risk pregnant women submitted to obstetrical morphological ultrasound at the 'Instituto de Medicina Integral Professor Fernando Figueira (I.M.I.P.)', from March 2002 to March 2006. Prenatal diagnosis was confirmed after birth. Socio demographic characteristics and pre and postnatal frequencies of fetal anomalies were the variables studied. Agreement between pre and postnatal diagnoses from congenital anomalies were evaluated with the Kappa indicator. Youden's test was applied to validate prenatal ultrasound diagnoses. Nine hundred and eighty nine patients were eligible. After evaluation of inclusion and exclusion criteria 457 patients were included in study. The average maternal age was 24.8 + 6.5 years. Fetal anomaly diagnoses postnatal were confirmed in 257 (56.2%) pregnant women. Prenatal ultrasound diagnosis of fetal anomalies disclosed 96% of sensibility and 79% of specificity, good agreement (K=0.76) between pre and postnatal diagnoses when compared to postnatal results and good diagnostic validity (Y=0.75). Prenatal diagnoses of fetal anomalies at a Fetal Medicine center in Pernambuco demonstrate good sensibility, specificity, agreement pre and postnatal and good diagnostic validity. | {
"pile_set_name": "PubMed Abstracts"
} |
A computational framework for ultra-high resolution cortical segmentation at 7Tesla.
This paper presents a computational framework for whole brain segmentation of 7Tesla magnetic resonance images able to handle ultra-high resolution data. The approach combines multi-object topology-preserving deformable models with shape and intensity atlases to encode prior anatomical knowledge in a computationally efficient algorithm. Experimental validation on simulated and real brain images shows accuracy and robustness of the method and demonstrates the benefits of an increased processing resolution. | {
"pile_set_name": "PubMed Abstracts"
} |
Asthma-like syndrome in school children after accidental exposure to xylene and methylene diphenyl diisocyanate.
We assessed the signs and symptoms, pulmonary function changes and residual chemical body burden of school children in the vicinity of an accidental exposure to volatile xylene and methylene diphenyl diisocyanate (MDI). After the exposure episode, children with significant symptoms after MDI exposure (e.g., dizziness, nausea, sore throat, and breathing difficulties) were sent to nearby emergency medical units for evaluation and admission if necessary. Clinical work-up included pulmonary function tests and measurement of residual MDI in the body by high-performance liquid chromatography analysis of urine. 203 students appeared to develop symptoms associated with contaminant exposure, and 173 affected students were sent to nearby emergency units. In the subsequent surveillance, 22 of 203 affected students (10.8%) revealed a positive history of asthma, which was strongly correlated with the incidence of dyspnea arising from the incident. For children with no previous history of asthma, 60.8% (110 of 181) complained of dyspnea during the episode, and 16.2% required inhaled bronchodilator therapy at the emergency medical units for relief of wheezing symptoms. In a simulation, we found the raw material used for tract surfacing, primarily MDI dissolved in xylene, to be present at a concentration (870 ppm w/w) more than 8000-fold the level defined as safe for a working environment. We have detected a direct cause-effect relationship between the accidental spillage of MDI and the appearance of an acute asthma-like syndrome among previously unexposed school children. | {
"pile_set_name": "PubMed Abstracts"
} |
Hepatobiliary disease in neonatal lupus: prevalence and clinical characteristics in cases enrolled in a national registry.
To extend the information base on the hepatobiliary manifestations of neonatal lupus erythematosus (NLE) with regard to frequency of occurrence, clinical characteristics, and outcome. Review of records from the Research Registry for Neonatal Lupus. Nineteen (9%) of 219 patients who had NLE and were enrolled in a national registry had probable or possible NLE hepatobiliary disease. In 16 cases, hepatobiliary disease occurred in addition to cardiac or cutaneous NLE. In 3 cases, hepatobiliary disease occurred as the sole clinical manifestation of NLE. Three clinical variants of hepatobiliary disease were observed: 1) severe liver failure present during gestation or in the neonatal period, often with the phenotype of neonatal iron storage disease; 2) conjugated hyperbilirubinemia with mild or no elevations of aminotransferases, occurring in the first few weeks of life; and 3) mild elevations of aminotransferases occurring at approximately 2 to 3 months of life. The prognosis for the children in the last 2 categories is excellent. Hepatobiliary disease is a relatively common finding in NLE and can be the sole clinical manifestation of NLE. Clinicians should be aware of the broad range of hepatobiliary disease that may occur in children with NLE. | {
"pile_set_name": "PubMed Abstracts"
} |
Optimal use of adjuvant chemotherapy in stage II colorectal cancer.
The prognosis of stage II colorectal cancer varies. Whether or not to perform adjuvant chemotherapy on patients with stage II colorectal cancer is a controversial issue. The aims of this study were to identify relevant risk factors for the prognosis of stage II colorectal cancer and to evaluate the need for adjuvant chemotherapy. Between January 2000 and January 2005, 443 patients with stage II colorectal cancer who had received radical surgery at the Sun Yat-sen University Cancer Center were retrospectively analyzed. The overall survival rates and survival curves were analyzed using the Kaplan-Meier method and log-rank test. Univariate and multivariate prognostic analyses were performed using the Cox regression model. Patients with certain important risk factors were analyzed according to whether they received adjuvant chemotherapy, and four chemotherapeutic regimens were classified into sub-groups and analyzed. Univariate analyses showed that intestinal obstruction or perforation, type 2 diabetes mellitus, an inadequate surgical margin, and sampling of less than 12 lymph nodes were risk factors that correlated with poor prognosis. Patients with an intestinal obstruction or perforation and insufficient lymph node samples achieved higher 5-year survival rates with adjuvant chemotherapy than with surgery alone. Intestinal obstruction or perforation, sampling of less than 12 lymph nodes, and inadequate surgical margins were identified as risk factors for poor survival, and patients with either of the first two factors benefited from adjuvant chemotherapy. Moreover, the use of capecitabine alone may be insufficient for patients with an intestinal obstruction or perforation. | {
"pile_set_name": "PubMed Abstracts"
} |
Terrorism and mental illness: is there a relationship?
This article examines the connections between mental illness and terrorism. Most social scientists have discounted a causal relationship between mental illness and terrorism. This is not necessarily always the case within terrorism studies, the media, or political circles where the psychology of terrorism is often expressed in the language of mentalisms, and theories of pathologisation continue to exist. This article reaffirms the view that apart from certain pathological cases, there is no causal connection between an individual's mental disorder and engagement in terrorist activity. The individual terrorist's motivations can be explained by other factors, including behavioural psychology. However, there may be a connection between an individual engaging in terrorist activity and developing a mental disorder[s]. Certain stressors that occur because of terrorist activity may result in psychological disturbance in terrorist individuals. These factors may partially explain terrorist group instability and should be taken into account when detaining and interrogating terrorist suspects. | {
"pile_set_name": "PubMed Abstracts"
} |
[Epidemiologic surveillance of hydatidosis in the Patagonian region, Argentina: 1983-1988].
The results of a control program of hydatid disease in five jurisdictions of the Patagonian Region in Argentina are presented. The information is glossed in rates of infection in man (95 x 100,000 in 1988 and 15 x 100,000 in 1983), dogs (21.6% in 1983 and 3.1% in 1988) and sheep (16.6% in 1983 and 2.6% in 1988). Factors that condition the endemic situation of hydatidosis in the region are discussed. | {
"pile_set_name": "PubMed Abstracts"
} |
[The method of the simultaneous determination of the citrinin and ochratoxin A content in cereals and cereal products].
The simplifyed method of simultaneous determination of the citrinin (CT) and ochratoxin A (OTA) in cereals is described. The extraction of mycotoxins was carried out by small volumes of solvents (water/aceton/hexane) without additional sample clean up and concentration. The extracts were analysed by HPLC using mixture of methanol/ethylacetate/phosphoric acid, pH 2.2 as a mobile phase and fluorescence detection (lamda ex 330 nm, lamda em 495 nm). The reliability and reproducibility of results were improved by usage of internal standard--methyl-derivative of ochratoxin A. Average recovery of CT and OTA was 70%, relative standard deviation 12% and 7%, respectively, limit of detection 0.003 mg/kg. | {
"pile_set_name": "PubMed Abstracts"
} |
Systematic error of lidar profiles caused by a polarization-dependent receiver transmission: quantification and error correction scheme.
Signals of many types of aerosol lidars can be affected with a significant systematic error, if depolarizing scatterers are present in the atmosphere. That error is caused by a polarization-dependent receiver transmission. In this contribution we present an estimation of the magnitude of this systematic error. We show that lidar signals can be biased by more than 20%, if linearly polarized laser light is emitted, if both polarization components of the backscattered light are measured with a single detection channel, and if the receiver transmissions for these two polarization components differ by more than 50%. This signal bias increases with increasing ratio between the two transmission values (transmission ratio) or with the volume depolarization ratio of the scatterers. The resulting error of the particle backscatter coefficient increases with decreasing backscatter ratio. If the particle backscatter coefficients are to have an accuracy better than 5%, the transmission ratio has to be in the range between 0.85 and 1.15. We present a method to correct the measured signals for this bias. We demonstrate an experimental method for the determination of the transmission ratio. We use collocated measurements of a lidar system strongly affected by this signal bias and an unbiased reference system to verify the applicability of the correction scheme. The errors in the case of no correction are illustrated with example measurements of fresh Saharan dust. | {
"pile_set_name": "PubMed Abstracts"
} |
OBSERVATIONS ON PHOTOHETEROTROPHY IN A MARINE DIATOM(1).
An unidentified species of the diatom genus Cocconeis has been isolated from the sediments of Biscayne Bay, Florida. The organism is capable of utilizing a range of organic substrates, including lactate, in the light but not in the dark, as shown by growth studies. Information is included on changes in cell carbon, nitrogen, and chlorophyll a during growth in batch culture. Data obtained on the kinetics of uptake of lactate and glucose raise questions on the possible ecological significance of photoheterotrophy among marine microalgae, particularly those in estuaries. | {
"pile_set_name": "PubMed Abstracts"
} |
Apolipoprotein E, smooth muscle cells and the pathogenesis of cerebral amyloid angiopathy: the potential role of impaired cerebrovascular A beta clearance.
Cerebral amyloid angiopathy (CAA) is caused by the deposition of beta-amyloid (A beta) in Alzheimer disease brains. It also occurs isolated, representing a major cause for cerebral hemorrhage in the elderly. The E4 genotype of apolipoprotein E (ApoE) is a risk factor for CAA; however, the molecular mechanism underlying this genetic association is unknown. Various findings suggest that cerebrovascular A beta is derived from the soluble A beta contained in the cortical extracellular space or the cerebrospinal fluid (CSF) that communicates and surrounds small cortical or leptomeningeal vessels. CAA deposits are always intimately associated with smooth muscle cells (SMCs) or SMC-derived pericytes. As we have previously reported, SMCs internalize A beta in vitro via a lipoprotein pathway involving ApoE and the low-density lipoprotein receptor family. Internalized A beta is subsequently located to lysosomes, suggesting its intracellular degradation. We show that A beta is internalized via multiple pathways, because class A and class B scavenger receptors are also colocalized to A beta-containing endosomes in SMCs, and A beta uptake is inhibited by various scavenger receptor antagonists. It has been recently shown for different cell types that the cellular uptake of ApoE is more efficient for the ApoE3 isoform when compared to ApoE4 and that this isoform-specific difference depends on the presence of heparan sulfate proteoglycan (HSPG). HSPG is produced by SMCs and promotes A beta fibrillogenesis. We propose a pathogenetic model of CAA, in which the ApoE- and HSPG-mediated clearance of CSF-derived A beta peptides by SMCs protects the vascular extracellular matrix against critical A beta concentrations. Impairment of this pathway or its reduced efficiency in carriers of the ApoE4 genotype may increase the risk of developing CAA. | {
"pile_set_name": "PubMed Abstracts"
} |
Interaction of rhodopsin with the G-protein, transducin.
Rhodopsin, upon activation by light, transduces the photon signal by activation of the G-protein, transducin. The well-studied rhodopsin/transducin system serves as a model for the understanding of signal transduction by the large class of G-protein-coupled receptors. The interactive form of rhodopsin, R*, is conformationally similar or identical to rhodopsin's photolysis intermediate Metarhodopsin II (MII). Formation of MII requires deprotonation of rhodopsin's protonated Schiff base which appears to facilitate some opening of the rhodopsin structure. This allows a change in conformation at rhodopsin's cytoplasmic surface that provides binding sites for transducin. Rhodopsin's 2nd, 3rd and putative 4th cytoplasmic loops bind transducin at sites including transducin's 5 kDa carboxyl-terminal region. Site-specific mutagenesis of rhodopsin is being used to distinguish sites on rhodopsin's surface that are important in binding transducin from those that function in activating transducin. These observations are consistent with and extend studies on the action of other G-protein-coupled receptors and their interactions with their respective G proteins. | {
"pile_set_name": "PubMed Abstracts"
} |
Current status of zirconia implants in dentistry: preclinical tests.
This systematic review aimed to provide an overview of zirconia implants as well as regarding the outcome of the implant-restorative complex in preclinical studies. An electronic search of the literature prior to July 2017 was performed to identify all articles related to preclinical research on zirconia implants. The search was conducted using MEDLINE (National Library of Medicine) and PubMed without restrictions concerning the date of publication. The search terminology included: zirconia implant, osseointegration, bone-to-implant contact, soft tissue, histology, histomorphometry, surface modification, surface roughness, surface characteristics, and restoration (connecting multiple keywords with AND, OR). Fifty-seven studies were finally selected from an initial yield of 654 titles, and the data were extracted. The identified preclinical studies focused on several aspects related to zirconia implants, namely biocompatibility, mechanical properties, implant design, osseointegration capacity, soft tissue response, and restorative options. Due to heterogeneity of the studies, a meta-analysis was not possible. The most frequently used zirconia material for the fabrication of implants is yttria-stabilized tetragonal zirconia polycrystal. The resistance-to-fracture for zirconia implants ranged between 516-2044N. The mostly investigated parameter was osseointegration, which is compared to that of titanium. A lack of evidence was found with other parameters. Due to its good biocompatibility as well as favorable physical and mechanical properties, zirconia implants are a potential alternative to titanium implants. However, knowledge regarding the implant-restorative complex and related aspects is still immature to recommend its application for daily practice. | {
"pile_set_name": "PubMed Abstracts"
} |
Current ophthalmologic treatment strategies for acute and chronic Stevens-Johnson syndrome and toxic epidermal necrolysis.
To review the newer, effective ophthalmologic treatments for acute Stevens-Johnson syndrome (SJS) as well as the emerging treatment options for patients with chronic, severe ocular surface damage from the disease. Amniotic membrane transplantation (AMT) applied to the eyes and eyelids in the acute phase of SJS can prevent the devastating scarring and visual problems that characterize the chronic phase of the disease. The severity of ocular inflammation in the acute phase does not always correlate to the severity of skin and systemic involvement. Thus, it is crucial that all patients with SJS be evaluated by an ophthalmologist familiar with the current management of the disease, the potential urgency of the situation and the option of AMT. Although challenging, the severe, chronic ocular problems of SJS can be at least partially alleviated with autologous serum drops, mucous membrane grafting to replace scarred tarsal conjunctiva, specialized contact lenses (PROSE), conjunctival replacement surgery (COMET), limbal stem cell transplantation and kerotoprostheses. Early AMT is an effective treatment of acute SJS. Emerging treatments offer increased hope for those who have already suffered damage from SJS, but emphasis on the prevention of damage in the acute phase is most crucial. | {
"pile_set_name": "PubMed Abstracts"
} |
The effect of the resistive loading of inspiration and expiration on pulmonary stretch receptor discharge.
Anesthetized, spontaneously breathing cats were used to examine the hypothesized role of slowly adapting pulmonary stretch receptors (PSR) in the control of breath duration. Initially, graded inspiratory and expiratory resistive loads were added to elucidate the inspiratory and expiratory volume-time relationship with both vagi intact. Unilateral vagotomy increased the slope of the VI--TI relationship indicating a reduction of the volume related modulation of TI. PSR frequency (fPSR) at end-inspiration also progressively decreased resulting in a fPSR--TI relationship qualitatively similar to the VI--TI curve. Expiratory resistive loading also produced an increased slope for the VE--TE relationship when the right vagus nerve was severed. The prolongation of TE was associated with a progressive increase in the number of PSR discharges during the loaded expiration. These results support the hypothesized role of PSR in the vagally mediated prolongation of TI and TE during resistive loading. In a subsequent series of experiments, the changes in fPSR were correlated with the tidal volume and transpulmonary pressure (PTP) changes. The fPSR was linearly related to PTP during both eupnic and loaded breathing. When fPSR was plotted against volume, a clockwise hysteresis was observed. These results suggest that in the spontaneously breathing cat, intrathoracic PSR frequency varies as a function of the transmural pressure across the airways. | {
"pile_set_name": "PubMed Abstracts"
} |
[Handicapped children and children at risk for handicaps in Bavarian integrated kindergartens--initial results of a survey].
The survey "integrative preschool education - an inquiry in Bavarian preschools/kindergartens" aimed to obtain a representative picture of the situation of handicapped children in regular preschool settings - as opposed to special institutions. In this paper we present information regarding distribution among different group-sizes as well as different defining/diagnosing systems. We also investigated the influence of certain ecological conditions such as size of place where the preschool is situated and distance between preschool and the nearest special institution. Furthermore we gained insight into the conditions which hinder and those which promote the admission of handicapped children. | {
"pile_set_name": "PubMed Abstracts"
} |
Synovial lymphoid neogenetic factors in Behçet's synovitis: do they play a role in self-limiting and subacute course of arthritis?
Lymphoid neogenesis seems to play an important role in the persistence of chronic inflammation and has been shown in various disorders characterized by chronic inflammation including rheumatoid arthritis. Arthritis of Behçet's disease is characterized by non-erosive arthritis in which the disease course is considered to be subacute and self limiting. However, molecular mechanisms underlying those features of Behçet's arthritis have not been defined yet. In order to determine the contribution of lymphoid neogenesis in the disease course of Behçet's arthritis, we investigated the synovial fluid (SF) levels of CXCL 12, CXCL 13, CCL 21 homeostatic chemokines and the percentage of SF naive lymphocytes expressing their receptors such as CXCR4+ and CCR7+. We further measured the SF TGF-Beta and INF-Beta levels which are known to contribute lymphoid neogenesis via leading persistent expression of CXCR4 on T cells and inhibiting T cell apoptosis, respectively. Fifty-one [15 BD, 17 RA, and 19 osteoarthritis (OA)] patients with at least one- sided knee arthritis were enrolled in the study. Patients with BD constituted the study group, and RA, OA patients were used as positive and negative control groups, respectively. The SF levels of CXCL 12, CXCL 13, CCL 21, TGF-Beta and INF-Beta were measured by ELISA. CXCR4, CCR7 chemokine receptors on SF lymphocytes were tested by Flow- cytometry. Kruskal-Wallis test and Mann-Whitney U test were used for statistical analysis where appropriate. Synovial fluid CCL 21 levels were found to be increased in RA patients as compared to BD and OA patients (p = 0.003, and p = 0.013, respectively). No significant difference was detected between BD and OA patients with respect to CCL 21 levels. Both CXCL 12 and CXCL 13 SF levels were found to be higher in RA and BD patients as compared to OA patients (CXCL-12; p = 0.012, and p = 0.024), (CXCL 13; p < 0.001, and p = 0.007). However, no difference with regard to SF levels of both CXCL 12 and CXCL 13 were found between RA and BD patients. Percentages of both CD3+CXCR4+ lymphocytes and CD3+CCR7+ lymphocytes in the SF of RA patients were detected to be increased as compared to those of BD and OA patients (CD3+CXCR4+; p = 0.019, p = 0.048, respectively), (CD3+CCR7+; p = 0.023, p = 0.001, respectively). However, no differences with respect to the percentages of SF lymphocytes expressing CD3+CXCR4+ or CD3+CCR7+ were found between BD and OA patients. Both TGF-Beta and INF-Beta SF levels were found to be higher in RA patients as compared to BD and OA patients (TGF-Beta; p = 0.041, and p = 0.003), (INF-Beta; p = 0.012, and p = 0.016). However, no differences with regard to SF levels of both TGF-Beta and INF-Beta were found between BD and OA patients. Considering the subacute, self limiting and non-erosive course of arthritis observed in BD, our finding of detection of lower levels of CCL21 and TGF-Beta1 and IFN-Beta in BD patients, seems to prevent the development of LN and chronic inflammation in Behçet's synovitis. In support of this view, percentages of SF naïve T lymphocytes were found to be lower in BD patients comparing with those of the RA. Absence of tertiary lymphoid structures in BD patients, may explain the spontaneous resolution of Behçet's arthritis in most of the cases. | {
"pile_set_name": "PubMed Abstracts"
} |
Evidence for stimulation of adenylyl cyclase by an activated G(s) heterotrimer in cell membranes: an experimental method for controlling the G(s) subunit composition of cell membranes.
Heterotrimeric (alphabetagamma) G(s) mediates agonist-induced stimulation of adenylyl cyclase (AC). Cholera toxin (CTx) will ADP-ribosylate the alpha-subunit of G(s) (G(s)alpha). G(s)alpha-deficient cyc(-) membranes were "stripped" of Gbeta. When the stripped cyc(-) were incubated with G(s)alpha and/or Gbetagamma, each was incorporated into the membranes independently of the other. Both G(s)alpha and Gbetagamma had to be present in the membranes, and they had to be able to form a heterotrimer in order for CTx to ADP-ribosylate G(s)alpha, indicating that the membrane bound G(s) heterotrimer is a substrate for CTx, but the G(s)alpha subunit by itself is not. When G(s)alpha was completely and irreversibly activated with GTPgammaS and incorporated into stripped cyc(-), it was a poor substrate for CTx and a weak stimulator of AC unless Gbetagamma was also incorporated. Furthermore, the level of AC stimulation corresponded to the amount of G(s) heterotrimer that was formed in the membranes from GTPgammaS-activated G(s)alpha and Gbetagamma. These data suggest that AC is stimulated by an activated G(s) heterotrimer in cell membranes. | {
"pile_set_name": "PubMed Abstracts"
} |
Regulatory T cells suppress systemic and mucosal immune activation to control intestinal inflammation.
The gastrointestinal (GI) tract is the main interface where the body encounters exogenous antigens. It is crucial that the local response here is tightly regulated to avoid an immune reaction against dietary antigens and commensal flora while still mounting an efficient defense against pathogens. Faults in establishing intestinal tolerance can lead to disease, inducing local and often also systemic inflammation. Studies in human as well as in animal models suggest a role for regulatory T cells (Tregs) in maintaining intestinal homeostasis. Transfer of Tregs can not only prevent the development of colitis in animal models but also cure established disease, acting both systemically and at the site of inflammation. In this review, we discuss the major regulatory pathways, including transforming growth factor-beta (TGF-beta), interleukin-10 (IL-10), and cytotoxic T-lymphocyte antigen-4 (CTLA-4), and their role in Treg-mediated control of systemic and mucosal responses. In addition, we give an overview of the known mechanisms of lymphocyte migration to the intestine and discuss how CD103 expression can influence the balance between regulatory and effector T cells. Further understanding of the factors that control the activity of Tregs in different immune compartments may facilitate the design of strategies to target regulation in a tissue-specific way. | {
"pile_set_name": "PubMed Abstracts"
} |
The alchemy of training.
Training in Zurich has its own special character but also is marked by the very fact that it is in Zurich. Zurich radiates its own distinct energy and carries a specific historical significance in the world of analytical psychology. This, like all things with psychic energy, has a spectrum of meaning. This spectrum, as well as the 'spirit' of the place, will be critically examined, taking into account the 'blessings' and 'curses' of such genius loci. Training in Zurich is experientially based and is first and foremost an initiation: an initiation into symbolic life, or rather life where symbol plays an important role. Training is understood to involve a transformation of one's self, much like the 8th century alchemist Morienus Romanus understood the opus as a 'human transformation system'. It is not merely an education. The requirement of 'immersion' is core to the experience of becoming an analyst in Zurich and this sets up a valuable discomfort between rational intellectual learning and intuitive experience, between knowing and not-knowing. How does this dis-serve the making of an analyst? What is implicit in this immersion and its discomfort? Does it have a role in today's emphasis on clinical and empirical training? Does Zurich still offer something unique and valuable in the world of training, or is it passé? From these questions, the dichotomy of what is 'urgent' and 'essential' in training will be examined. | {
"pile_set_name": "PubMed Abstracts"
} |
Laboratory test reactor for the investigation of liquid reducing agents in the selective catalytic reduction of NOx.
A test reactor was designed and built for investigating liquid reducing agents in the selective catalytic reduction (SCR) process in the laboratory. The design of the experimental setup is described in detail and its performance was evaluated. Using a glass nebulizer, liquid reducing agents were sprayed directly onto a catalyst positioned in a heated glass reactor with a length of 250 mm and an internal diameter of 20.4 mm or 40 mm. Model exhaust gases were mixed from individual gas components and were heated up to 450 °C in a heat exchanger before entering the reactor. The off-gas was analyzed using two complimentary techniques, a multi-component online FTIR gas analysis and a liquid quench gas absorption setup, to detect higher molecular compounds and aerosols. Due to the versatility of construction, processes not related to SCR, but involving three-phase reactions with gases, liquids and a catalyst, can also be investigated. | {
"pile_set_name": "PubMed Abstracts"
} |
The interaction between halogenated anaesthetics and bacteriorhodopsin in purple membranes as examined by intrinsic ultraviolet fluorescence.
In the presence of halogenated general anaesthetics such as enflurane and halothane, the spectral properties of the bacteriorhodopsin pigment contained in the purple membranes of Halobacterium halobium are strongly modified. It is reversibly transformed into a red-coloured species absorbing maximally at 480 nm, at the expense of its characteristic 570-nm absorption band. The ultraviolet fluorescence of bacteriorhodopsin has been used to probe the structural modifications that are reflected by this spectral change. Our results show that they are very small and do not perturb the energy transfer dynamics which take place between the aromatic amino acid residues and the retinyl chromophore. The fluorescence properties of anaesthetic-treated bacteriorhodopsin are dominated by the quenching properties of the halogenated hydrocarbon, which are obvious even at anaesthetic concentrations under those needed to induce a spectral change in the bacteriorhodopsin chromophore. This does not rule out direct interaction between anaesthetics and bacteriorhodopsin, but it indicates that the chromophoric site might well not be their primary target. | {
"pile_set_name": "PubMed Abstracts"
} |
Pain management: the global connection.
Understanding pain and the cultural perceptions patients and staff hold about it is crucial to developing a pain management program that complies with Joint Commission requirements. | {
"pile_set_name": "PubMed Abstracts"
} |
Prognosis and sequela in patients with childhood craniopharyngioma -- results of HIT-ENDO and update on KRANIOPHARYNGEOM 2000.
The overall survival rate of patients with craniopharyngioma, an embryogenic malformation, is high (0.92 +/- 0.03). However, there is considerable morbidity and impaired quality of life, even when the tumor can be completely resected. Aim of our study was to analyze risk factors for severe obesity (body mass index [BMI] > or = 7 SD) and the prognosis of severely obese survivors of childhood craniopharyngioma recruited in our cross-sectional study on 183 patients (HIT-ENDO) and in the prospective study KRANIOPHARYNGEOM 2000. Severe obesity (BMI > or = 7 SD) was present in 16 % of patients and associated with higher tumor volume (p < 0.05), more frequent neurosurgical interventions (p < 0.05) and a higher rate of hypothalamic involvement (p < 0.001). Self-assessed functional capacity (FMH) was lower (p < 0.001) in severely obese survivors (FMH: median 33, range: 4-64) when compared with normal weight patients (FMH: 50; 1-95). Overall survival (20 years) was lower (p = 0.034) in patients with severe obesity in comparison to patients with moderate obesity and normal weight. Cardiovascular morbidity and mortality was high in severely obese patients. Between 10/01 and 03/04 fifty-seven patients (27 female/30 male) were recruited in KRANIOPHARYNGEOM 2000 (www.kraniopharyngeom.com). The impact of tumor localization (3.2 % intrasellar, 22.6 % suprasellar, 74.2 % combined intra/suprasellar; 51.6 % hypothalamic involvement) and treatment (26 complete resections, 22 partial resections followed by irradiation in one case; primary irradiation after biopsy in one case) on quality of life, functional capacity and prognosis will be evaluated longitudinally and prospectively. | {
"pile_set_name": "PubMed Abstracts"
} |
Mutations affecting extracellular protease production in the filamentous fungus Aspergillus nidulans.
The extracellular proteases of Aspergillus nidulans are known to be regulated by carbon, nitrogen and sulphur metabolite repression. In this study, a mutant with reduced levels of extracellular protease was isolated by screening for loss of halo production on milk plates. Genetic analysis of the mutant showed that it contains a single, recessive mutation, in a gene which we have designated xprE, located on chromosome VI. The xprE1 mutation affected the production of extracellular proteases in response to carbon, nitrogen and, to a lesser extent, sulphur limitation. Three reversion mutations, xprF1, xprF2 and xprG1, which suppress xprE1, were characterised. Both xprF and xprG map to chromosome VII but the two genes are unlinked. The xprF1, xprF2 and xprG1 mutants showed high levels of milk-clearing activity on medium containing milk as a carbon source but reduced growth on a number of nitrogen sources. Evidence is presented that the xprE1 and xprG1 mutations alter expression of more than one protease and affect levels of alkaline protease gene mRNA. | {
"pile_set_name": "PubMed Abstracts"
} |
Variable presentation of retrocaval ureter: Report of four cases and review of literature.
Retrocaval ureter is a rare anomaly in which the ureter passes behind the inferior vena cava due to abnormal embryogenesis. Very few cases have been reported from Africa. Although the anomaly is congenital, patients become symptomatic in the third or fourth decade of life. We reviewed the records of four patients with the diagnosis of retrocaval ureter and managed in our centre between January 2010 and December 2016. Three patients presented with recurrent colicky right flank pain while one was asymptomatic. Two patients each had Type I and Type II retrocaval ureters, respectively. Surgical repairs were achieved in the three symptomatic cases and recovery was uneventful. Retrocaval ureter, though congenital, manifests in young adults and it may be symptomatic. Pre-operative diagnosis may be difficult when the lesion is high and mimics pelviureteric junction obstruction. Thus, a high index of suspicion is required for pre-operative diagnosis. Under-reporting and asymptomatic cases may account for the low incidence. | {
"pile_set_name": "PubMed Abstracts"
} |
Treatment of peripheral vascular diseases--basic data from the nationwide vascular registry FINNVASC.
A national vascular registry, the FINNVASC registry, was introduced in Finland in the beginning of 1991. The registry covers the whole country--Finland has a population of 5.0 million habitants. During the first two years 8047 procedures were registered together with a 30-day follow-up. The number of surgical operations amounted to 5494 (68.3%), while the remaining 2553 procedures were endovascular. 22% of the surgical and 6.9% of the endovascular procedures were made as emergencies; about 10% of any procedure were reoperations. The indications for surgery were chronic limb ischaemia in 40.2% and acute limb ischaemia in 11.9%. Surgery of abdominal aortic aneurysm was indicated in 13.1% and surgical procedures in the aortocervical region in 13.3%. Access surgery was performed in 8.0% of the patients. Chronic limb ischaemia was the main indication, 92.1%, for the endovascular procedures, and renal artery stenosis was the second most frequent endovascular procedure, 2.7%. The mean length of hospital stay for surgical patients was 10.7 days and for patients treated with endovascular procedures 4.7 days. Patient outcome of 30 days of follow-up was comparable with the reports from other centres. | {
"pile_set_name": "PubMed Abstracts"
} |
[The value of human-genetic studies for the evaluation of the etiology of malocclusions and bite irregularities].
Researches in which entire families as well as twins are subjected to careful examinations are rapidly assuming considerable importance within the framework of studies made to investigate the causes of irregular dentural characteristics. The author emphasizes the need for making genetic examinations of orthodontic patients, the results of which can be used to find the most favorable method of therapy. The author, on the basis of observations made on monozygotic and dizygotic twins, presents a number of data on the behavior of dental, dental arcade, and cephalometric dimensions as well as some dysgnathic characteristics. Special attention is given to twins with congenital cheiloschisis, gnathoschisis, and palatoschisis, and it is possible to show that the role played by hereditary factors in the etiology of such maldevelopments is not inconsiderable. | {
"pile_set_name": "PubMed Abstracts"
} |
[Biosorption of heavy metals by bacteria isolated from activated sludge].
In this paper, biosorption efficiency of heavy metals by bacteria isolated from activated sludge was tested. It was shown that Pseudomonas pseudoalcaligenes and Micrococcus luteus GC subgroup B had high biosorption capacities for Cu(II) and Pb(II) ions. The adsorption of the two ions on Pseudomonas pseudoalcaligenes and Micrococcus luteus GC subgroup B exhibited Langmuir adsorption behavior with regression coefficient of 0.99. The pH value was the most important effect factor on biosorption of metal ions. The biosorption kinetic curves of Cu(II) and Pb(II) ions by cells showed two processes: rapid sorption on cell surface and slowly longer-term transformation into cells. Cu(II) ion can be desorbed effectively from cells of Micrococcus luteus GC subgroup B. | {
"pile_set_name": "PubMed Abstracts"
} |
Dual Therapy for Refractory Overactive Bladder in Children: A Prospective Open-Label Study.
Mirabegron (β3 adrenoreceptor agonist) is a new molecule with a mechanism of action distinct from antimuscarinics. Combination therapy with solifenacin was recently studied in an adult population. We evaluated the efficacy and safety of mirabegron as add-on therapy to treat urinary incontinence in children with idiopathic overactive bladder refractory/intolerant to antimuscarinics. A prospective off-label study using add-on regimens of mirabegron was conducted in pediatric patients presenting with no symptom improvement while undergoing intensive behavioral and medical therapies and/or significant side effects while undergoing antimuscarinic dose escalation. Our primary outcome was better reported efficacy than with the use of prior antimuscarinic monotherapy. Secondary end points were tolerability, safety and satisfaction. Efficacy and tolerability were assessed based on voiding diaries, post-void residuals, urine cultures, electrocardiograms and vital signs. Families were questioned regarding continence, side effects and compliance. Wilcoxon signed-rank test was used for statistical analysis. A total of 35 patients were recruited at a median age of 10.3 years and were administered add-on mirabegron for a median of 16.4 months. Median bladder capacity improved from 50% to 74% expected bladder capacity (p <0.001). Continence improved in all patients, with 12 being completely dry. Post-void residual was increased in 2 patients and 1 urinary tract infection was reported. Seven patients reported mild or moderate side effects, with 2 withdrawals because of side effects (1 patient) and post-void residual (1). Add-on mirabegron appears to be a safe alternative for children with refractory overactive bladder. Dual therapy is well tolerated and adjusted dose regimen appears safe in this first pediatric study. | {
"pile_set_name": "PubMed Abstracts"
} |
Synthesis and binding affinity of novel 3-aminoethyl-1-tetralones, potential atypical antipsychotics.
A series of 3-aminoethyl-1-tetralones, conformationally constrained higher homologues of haloperidol (standard for typical antipsychotic profile), have been obtained by a four-step route from valerolactone. Their binding affinities at dopamine D(2) and serotonin 5-HT2A and 5-HT2C receptors were determined, showing in some cases an atypical antipsychotic profile. | {
"pile_set_name": "PubMed Abstracts"
} |
Cortical afferent inhibition reflects cognitive impairment in obstructive sleep apnea syndrome: a TMS study.
Patients with obstructive sleep apnea syndrome (OSAS) show neurocognitive impairment, but the exact mechanisms that cause cognitive dysfunctions remain unknown. The cholinergic system is known to play a key role in all attentional processes and cognitive functions. A transcranial magnetic stimulation (TMS) protocol may give direct information about the function of some cholinergic circuits in the human brain; this technique relies on short latency afferent inhibition (SAI) of the motor cortex. The objective of this exploratory study was to test the hypothesis that impaired cognitive performances in OSAS patients are associated with a dysfunction of the cholinergic system, as assessed by SAI. We applied SAI technique in a group of 13 patients with OSAS and compared the data with those from a group of 13 age-matched healthy subjects. All the patients underwent a sleep study, an extensive neuropsychological evaluation, and TMS examination. Mean SAI was significantly reduced in our OSAS patients when compared with controls. The neuropsychological evaluation showed impairments in most cognitive areas in the OSAS patients. SAI values were strongly correlated with the neuropsychological test scores. These findings suggest that the cognitive deficits in OSAS may be, at least in part, secondary to alterations in cholinergic neurotransmission, presumably caused by nocturnal hypoxemia. TMS studies may shed light on the pathophysiological mechanisms of the cognitive disturbances in OSAS patients. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of the PIPC model on outcome measures of productivity and costs.
The purpose of this experimental research was to determine the effects of the Partners in Care Practice Model (PIPC) on the outcomes of productivity and costs. Over an 18-month study period, no significant differences were found in costs per patient day between the pilot and control units; however, significant differences were found in actual salaries, amount of time spent in documentation, and supply costs. Differences in costs and productivity reflected increased training costs and costs for additional supplies on the pilot unit. | {
"pile_set_name": "PubMed Abstracts"
} |
The second number-estimation elbow: Are visual numbers greater than 20 evaluated differently?
Numerosity perception has long been understood to be divided between subitizing and estimation. In a series of three experiments (total N = 113), a new number "elbow" point in the estimation of visual number for numerosities of about 20 dots is confirmed. Below 20, mean estimates are linear with a slope of about 1 and power-function exponents for numerosity estimation approximate unity, though estimate variance increases dramatically above about 6 elements. For numerosities above 20, estimates become increasingly compressed, such that power function exponents are much lower (e.g., 0.7) and are lower still when both ranges are estimated within the same experimental procedure. The experiments described here show that the location of the inflection point appears insensitive to the range of numbers estimated and to differences in density. | {
"pile_set_name": "PubMed Abstracts"
} |
A new look at the lipid composition of the plasma membrane of human blood platelets relative to the GPIIb/IIIa (integrin cxIIβ3) content.
The total lipids of the human platelet plasma membrane (HPPM) from 50 ml of blood of healthy subjects were extracted, quantified and related to the mass content of the major intrinsic membrane protein, the glycoprotein IIb/IIIa (GPIIb/IIIa). The HPPM total lipid/GPIIb/IIIa weight ratio determined was 5.40 ± 0.20, independently of the membrane washing procedure used, with the cholesterol/GPIIb/IIIa and phospholipid/GPIIb/IIIa molar ratios of 800 ± 50 and 1200 ± 40, respectively. If the distribution of lipids around each intrinsic protein were proportional to its mass, the lipids around a molecule of GPIIb/IIIa will occupy about 120 nm(2) of the membrane plane, which is about one and a half times the cross-sectional area of the extracellular head of GPIIb/IIIa, as estimated by electron microscopy. The lipid extracts were further subjected to thin-layer chromatography to separate and quantify the different phospholipid fractions, the free fatty acids and the neutral lipid fraction and the distribution of fatty acids in each fraction was determined by gas chromatography after methanolysis. The phospholipid molar distribution was SPM(22.3 ± 0.9%), PC(36.2 ± 1.0%), PE(24.9 ± 0.9%), PS(12.1 ± 0.6%) and PI(4.5 ± 0.4%) and the free fatty acid fraction represented 2.9 ± 0.4% of the total fatty acids in HPPM. The fatty acid chain length ranged from 14 to 24 carbons, comprising unsaturated fatty acids (47.3% molar per cent of the total) of which 40.7 ± 2.0% were monosaturated and 40.7 ± 0.9% tetraunsaturated. Palmitic, stearic, oleic and arachidonic acids represent 66% of the total fatty acids of HPPM, being: 68.9 ± 5.3% of palmitic acid and 63.3 ± 6.9% of oleic acid in PC; 50.9 ± 3.8% of arachidonic acid in PE; and 30.5 ± 2.4% of stearic acid in PS. We discuss the methodological modifications and the new data in relation with the major differences in HPPM lipid composition found in the literature. The data obtained provides a comprehensive and accurate description of the lipid composition of HPPM on which to rely as a reference for basic and medical studies. | {
"pile_set_name": "PubMed Abstracts"
} |
[A report of two familial cases of Michel syndrome (bilateral agenesis of the inner ear)].
In two siblings, wearing conventional hearing aid, presenting profound but not total congenital deafness, with no particular antecedents, the imaging destined to confirm the indication of a cochlear implant revealed a total bilateral agenesis of the inner ear. In one of the children, this imaging was confirmed by an exploration of the middle ear performed during a tonsillectomy that was otherwise necessary. We will summarize the literature concerning Michel's Disease, exceptional in its princeps form, and we will discuss the manner of action of conventional devices in these cases which are a priori without any sensorial element. | {
"pile_set_name": "PubMed Abstracts"
} |
Control of renin release by dietary NaCl in the rat.
The purpose of the present study is to determine whether changes of plasma renin activity (PRA) induced by dietary NaCl are mediated by a renal tubular mechanism or by a neural mechanism. Male Sprague-Dawley rats were placed on low-, normal-, or high-NaCl diets for 1 wk (n = 8 for each group). There were no group differences of glomerular filtration rate (GFR), renal plasma flow, Na+ or Cl- delivery to the loop, Na+ or Cl- reabsorption in the loop, Na+ or Cl- concentration in early distal tubular fluid, or Na+ or Cl- delivery to the early distal tubule. PRA of rats on normal NaCl (4.8 ng.ml-1.h-1 +/- 0.8) was greater (P less than 0.05) than that of rats on high NaCl (3.3 +/- 0.4) and less (P less than 0.05) than that of animals on low NaCl (9.1 +/- 1.8). To determine whether alterations of PRA by dietary NaCl might be related to low-pressure baroreceptors with vagal afferents, animals were bilaterally vagotomized after micropuncture. Forty-five minutes after vagotomy, PRA increased (P less than 0.05) on each of the diets, however, after vagotomy mean PRA in animals fed normal (10.9 +/- 1.8) and low NaCl (13.2 +/- 2.2) did not differ. Thus our results do not support the hypothesis that suppression of PRA by dietary NaCl loading is related to a renal tubular mechanism. A vagally mediated mechanism may contribute to renin suppression by dietary NaCl. | {
"pile_set_name": "PubMed Abstracts"
} |
Curcumin upregulates Nrf2 nuclear translocation and protects rat hepatic stellate cells against oxidative stress.
The present study aimed to investigate the protective role of curcumin against oxidative stress in rat hepatic stellate cells (HSCs)-T6, and to determine the possible underlying mechanisms. HSC-T6 cells were divided into three groups: Negative control group, oxidant-treated group and curcumin-treated group. Flow cytometry and spectrophotometry were used to measure the production of reactive oxygen species (ROS), and the levels of malondialdehyde (MDA) and glutathione (GSH). Immunocytochemistry and a radioimmunoassay were used to determine the expression of smooth muscle α-actin (α-SMA) and the secretion of extracellular matrix (ECM) molecules. In addition, western blotting and immunocytochemistry were used to determine the expression levels of nuclear factor-erythroid 2-related factor (Nrf2). Treatment with glucose oxidase (GO) significantly stimulated the formation of ROS and increased the production of MDA, as compared with the control cells; however, the production of GSH was only slightly increased. In addition, treatment with GO significantly promoted the expression of α-SMA and the secretion of ECM molecules. Conversely, treatment with curcumin significantly decreased the levels of ROS and MDA, and significantly increased the levels of GSH. Curcumin significantly inhibited the expression of α-SMA and decreased the secretion of ECM molecules. Furthermore, treatment with curcumin significantly increased the nuclear expression levels of Nrf2. These results indicated that curcumin may protect rat HSCs against oxidative stress and inhibit the GO-induced activation and secretion of ECM molecules in vitro. These effects were mediated by the upregulation of Nrf2 nuclear translocation. | {
"pile_set_name": "PubMed Abstracts"
} |
Forging Ahead: Update on Radiofrequency Ablation Technology and Techniques.
Innovations in radiofrequency ablation and non-ablative techniques have led to significant advances in addressing complex arrhythmogenic substrates for a variety of cardiac arrhythmias. Anatomical challenges, deep substrate and mid-myocardial locations may pose difficulties and decrease success rates using routine methods. In this review, we provide an update on novel radiofrequency technology and techniques including (1) high power, low duration ablation, (2) ablation facilitated by low-ionic irrigant and (3) bipolar ablation. In addition, we review emerging technologies including electroporation, needle catheter ablation and ablation with the lattice catheter. This article is protected by copyright. All rights reserved. | {
"pile_set_name": "PubMed Abstracts"
} |
H2O2 Exposure Affects Myotube Stiffness and Actin Filament Polymerization.
Skeletal muscles often experience oxidative stress in anaerobic metabolism and ischemia-reperfusion. This paper reports how oxidative stress affects the stiffness of cultured murine myotubes and their actin filaments polymerization dynamics. H2O2 was applied as an extrinsic oxidant to C2C12 myotubes. Atomic force microscopy results showed that short exposures to H2O2 apparently increased the stiffness of myotubes, but that long exposures made the cells softer. The turning point seemed to take place somewhere between 1 and 2 h of H2O2 exposure. We found that the stiffness change was probably due to actin filaments being favored for depolymerization after prolong H2O2 treatments, especially when the exposure duration exceeded 1 h and the exposure concentration reached 1.0 mM. Such depolymerization effect was associated with the down-regulation of thymosin beta 4, as well as the up-regulation of both cofilin2 and profilin1 after prolong H2O2 treatments. | {
"pile_set_name": "PubMed Abstracts"
} |
[Excessive allergy due to benzoic acid followed by anaphylactic shock. (author's transl)].
The case of a trained chemical worker highly sensitive to benzoic acid is described. He suffered from allergic reactions of increasing intensity while being constantly exposed to benzoic acid during work. After oral exposure to benzoic acid he suffered a severe anaphylactic shock and later on showed similar but milder reactions when eating food containing benzoic acid. No allergic reactions reoccurred avoiding every contact with benzoic acid especially observing a strict benzoic acid-free diet. | {
"pile_set_name": "PubMed Abstracts"
} |
Comparison of dental implant stabilities by impact response and resonance frequencies using artificial bone.
We compared implant stability as determined by the peak frequency from the impact response with the implant stability quotient (ISQ) by resonance frequency analysis (RFA) in various artificial bone conditions. The clinical bone conditions were simulated using an artificial bone material with different cortical thicknesses and trabecular densities. The artificial bone material was solid, rigid polyurethane. The polyurethane foam of 0.8g/cm(3) density was used for the cortical bone layer, and that of 0.08, 0.16, 0.24, 0.32, and 0.48g/cm(3) densities for the trabecular bone layer. The cortical bone material of 4 different thicknesses (1.4, 1.6, 1.8, and 2.0mm) was attached to the trabecular bone with varying density. Two types of dental implants (10 and 13mm lengths of 4.0mm diameter) were placed into the artificial bone blocks. An inductive sensor was used to measure the vibration caused by tapping the adapter-implant assembly. The peak frequency of the power spectrum of the impact response was used as the criterion for implant stability. The ISQ value was also measured for the same conditions. The stability, as measured by peak frequency (SPF) and ISQ value, increased as the trabecular density and the cortical density increased in linear regression analysis. The SPF and ISQ values were highly correlated with each other when the trabecular bone density and cortical bone thickness changed (Pearson correlation=0.90, p<0.01). The linear regression of the SPF with the cortical bone thickness showed higher goodness of fit (R(2) measure) than the ISQ value with the cortical bone thickness. The SPF could differentiate implantation conditions as many as the ISQ value when the trabecular bone density and the cortical density changed. However, the ISQ value was not consistent with the general stability tendency in some conditions. The SPF showed better consistency and differentiability with implant stability than the ISQ value by resonance frequency analysis in the various implantation conditions. | {
"pile_set_name": "PubMed Abstracts"
} |
Infertile polycystic ovary syndrome patients undergoing in vitro fertilization with the gonadotropin-releasing hormone-antagonist protocol: role of hyperandrogenism.
This retrospective cohort study is to assess the effects of hyperandrogenism (HA) in polycystic ovary syndrome (PCOS) patients with gonadotropin-releasing hormone (GnRH)-antagonist protocol during in vitro fertilization (IVF). Total 892 infertile Patients between 20 and 35 years of age with normal body mass index (BMI, 18.50 -24.99 kg/m2), including those with tubal factor infertility (control, n = 318), PCOS infertility with HA (HA PCOS, n = 244), and PCOS infertility without HA (non-HA PCOS, n = 330), were included. Number of retrieved oocytes was significantly higher and total Gonadotropin consumption was significant lower in the HA PCOS group, whereas abortion rate was significantly higher and live birth rate was significantly lower in the HA PCOS group, compared with the control and non-HA PCOS groups. In the HA PCOS group, the number of available embryos tended to be higher with no significance. The GnRH-antagonist protocol is more suitable for HA PCOS patients, with lower cost and more number of embryos available for transfer. Due to the high abortion rate and low live birth rate, a freeze-all approach might be a preferable option for HA PCOS patients so as to create a buffer for reducing androgen levels before transferring freeze-thawed embryos. | {
"pile_set_name": "PubMed Abstracts"
} |
Arterial metabolism of lipoproteins in relation to atherogenesis.
In this short review we have concentrated on the ways in which modification of LDL structure may account for foam cell formation. We have presented in vivo evidence as well as in vitro evidence supporting the proposition that modification of native LDL is a prerequisite for foam cell formation and atherogenesis. Actually, oxidized LDL can contribute to atherogenesis in other ways as well. Oxidized LDL is chemotactic for circulating monocytes, yet inhibits the motility of the tissue macrophage as shown by Quinn et al. Also, oxidized LDL is cytotoxic as discussed above and this could play a crucial role in the transition from the fatty streak lesion to the clinically more consequential fibrous plaque and complicated lesion. If further research supports the importance of LDL modification in atherogenesis, a whole new array of possibilities opens itself to us for intervention. Anything that interferes with the relevant modifications of the LDL structure would presumably be additive to interventions lowering the plasma concentration of LDL. At the moment, the only such intervention that appears to be feasible is prevention of LDL oxidation. Possibly we may find ways to interfere with immune mechanisms that are involved in some patients; conceivably we might be able to interfere with the aggregation of LDL with itself or with other complexes in the artery wall that appear also to favor initiation of the atherogenic process. | {
"pile_set_name": "PubMed Abstracts"
} |
Peripartum anesthetic management of a patient with brittle cornea syndrome.
Brittle cornea syndrome (BCS) is a rare autosomal recessive disease that affects the connective tissue. The syndrome is caused by genetic changes in the 4.7-Mb interval between the D16S3423 and D16S3425 markers on the 16q24 chromosome and mutations in the Zinc-Finger 469 gene (ZNF469). BCS is characterized by thin and fragile cornea that tends to perforate spontaneously or as a result of minor trauma to the eye. In addition, the patient usually suffers from hearing loss, mental retardation, hyperextensibility of skin and joints, as well as varying degrees of scoliosis. This phenotypical expression presents an interesting challenge to anesthetic care. We briefly present the perioperative management of a patient with BCS who underwent three cesarean sections. | {
"pile_set_name": "PubMed Abstracts"
} |
Intraoperative magnetic resonance imaging for neurosurgical procedures: anesthetic implications.
Intraoperative magnetic resonance imaging (IMRI) for tumor resection allows a neurosurgeon to pinpoint the exact location of the tumor before resection and to navigate to the tumor after the incision is made. Although the anesthetic management is not substantially different from that for other neurosurgical procedures, strategies to keep the patient and operating room personnel safe can be challenging. Because of the risk of injury by the strong force of the magnet, safety precautions with respect to anesthetic delivery must be taken. Ferrous objects must be removed and kept outside the operating room. Only MRI-compatible equipment is allowed in the MRI operating room. This includes the anesthesia machine, anesthesia cart, intubating equipment, monitors, stethoscopes, poles for intravenous solutions, and body warmers. Surgical equipment and instruments must be MRI-compatible. Absolute contraindications to entering the MRI suite include pacemakers, cochlear implants, certain cranial aneurysm clips, and metal joints or implants. Goals of anesthesia delivery during IMRI procedures include the following: (1) promoting the safety of patients and staff, (2) preventing MRI-associated accidents, (3) identifying potential equipment-related hazards, (4) recognizing limitations of physiologic monitoring, and (5) acknowledging other potential hazards such as noise. | {
"pile_set_name": "PubMed Abstracts"
} |
Effect of chiral symmetry on chaotic scattering from Majorana zero modes.
In many of the experimental systems that may host Majorana zero modes, a so-called chiral symmetry exists that protects overlapping zero modes from splitting up. This symmetry is operative in a superconducting nanowire that is narrower than the spin-orbit scattering length, and at the Dirac point of a superconductor-topological insulator heterostructure. Here we show that chiral symmetry strongly modifies the dynamical and spectral properties of a chaotic scatterer, even if it binds only a single zero mode. These properties are quantified by the Wigner-Smith time-delay matrix Q=-iℏS^{†}dS/dE, the Hermitian energy derivative of the scattering matrix, related to the density of states by ρ=(2πℏ)^{-1}TrQ. We compute the probability distribution of Q and ρ, dependent on the number ν of Majorana zero modes, in the chiral ensembles of random-matrix theory. Chiral symmetry is essential for a significant ν dependence. | {
"pile_set_name": "PubMed Abstracts"
} |
Enhanced drug transport from unilamellar to multilamellar liposomes induced by molecular recognition of their lipid membranes.
Unilamellar PC-based liposomes bearing a recognizable moiety were loaded either with the hydrophilic drug doxorubicin (DXR) or with the hydrophobic drug tamoxiphen (TMX) and allowed to interact with multilamellar PC-based liposomes bearing complementary recognizable groups. It has been established that, due to molecular recognition of these complementary liposomes, effective and fast transport of the drugs occurs from unilamellar to multilamellar liposomes. The transport of TMX is more effective compared to that of DXR. This behavior was observed for both PEGylated and non-PEGylated unilamellar liposomes, and it was attributed to the different sites of solubilization of the drugs in the unilamellar liposomes. PEGylation reduces the transport of both drugs since it inhibits to some extent the molecular recognition effectiveness of the complementary moieties. | {
"pile_set_name": "PubMed Abstracts"
} |
The penicillins.
The penicillin family of antibiotics remains an important part of our antimicrobial armamentarium. In general, these agents have bactericidal activity, excellent distribution throughout the body, low toxicity, and efficacy against infections caused by susceptible bacteria. The initial introduction of aqueous penicillin G for treatment of streptococcal and staphylococcal infections was an important pharmacologic landmark. The emergence of penicillinase-producing Staphylococcus aureus prompted the development of the penicillinase-resistant penicillins (for example, methicillin, oxacillin, and nafcillin), in which an acyl side chain prevented disruption of the beta-lactamase ring. Subsequently, the aminopenicillins (ampicillin, amoxicillin, and bacampicillin) were developed because of the need for gram-negative antimicrobial activity. Their spectrum initially included Escherichia coli, Proteus mirabilis, Shigella, Salmonella, Listeria, Haemophilus, and Neisseria. The search for a penicillin with additional antimicrobial activity against the Enterobacteriaceae and Pseudomonas aeruginosa led to the development of the carboxypenicillins (carbenicillin and ticarcillin) and the ureidopenicillins (mezlocillin, azlocillin, and piperacillin). Finally, the combination of a beta-lactamase inhibitor (clavulanic acid, sulbactam, or tazobactam) and an aminopenicillin, ticarcillin, or piperacillin has further extended their antibacterial spectra by inhibiting certain beta-lactamases (non-group 1) of resistant bacteria. The development of an ideal penicillin that is rapidly bactericidal, nonsensitizing, nontoxic, bioavailable, and resistant to beta-lactamases and that has a high affinity for penicillin-binding proteins remains the goal. | {
"pile_set_name": "PubMed Abstracts"
} |
Activation of locations in working memory in cats.
Cats saw an object appear and disappear at two successive locations; the movement of the object from one location to the other was not perceived but was indicated by indirect cues and the two disappearances were separated by a 0-sec or a 20-sec interval. Performance was poorer with the 0-sec than with the 20-sec interval. With the 0-sec interval, the percentages of search attempts made at the object's initial and final hiding locations did not differ whereas with the 20-sec interval, more search attempts were made at the final than at the initial location. These results provide additional support to Goulet, Doré and Rousseau's (1994) interpretation of cats' search behaviour in terms of activation of spatial locations in working memory. | {
"pile_set_name": "PubMed Abstracts"
} |
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