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Work-site casualties and environmental risk assessment on Polish vessels in the years 1960-1999.
To assess environmental risks of fatal incidents in the maritime work-site and guides for possible prevention. All casualties at the work-site in the years 1960-1999 among 25 525 seafarers employed on board Polish vessels were identified according to the ICD-10 classification. The main measure of risk: mean fatality rate per 1000 employees/year was calculated for external and internal causes of death. The impact of the maritime work environment was analysed. 668 seafarers died on board 405 Polish vessels, and 66 percent of deaths were from external causes. The mean fatality rate 1.17 per 1000, was five times higher for external (drowning, missing, other accidents) than for internal causes (circulatory system diseases): 412 deaths from accidents and 29 from disease. Despite modernization of the fleet, the environmental risk factors have strong impact on the mortality. The highest-risk groups were Baltic fishermen, seamen, deck crew, under 40 years of age. The risk of casualties in maritime industry exceeds the acceptable level of 1 death per 1000 employees per year. Prevention should concentrate on maritime catastrophes and work-related accidents. | {
"pile_set_name": "PubMed Abstracts"
} |
Enteropathogenic Escherichia coli Tir translocation and pedestal formation requires membrane cholesterol in the absence of bundle-forming pili.
Enteropathogenic Escherichia coli (EPEC) is a significant cause of paediatric diarrhoea worldwide. Virulence requires adherence to intestinal epithelial cells, mediated in part through type IV bundle-forming pili (BFP), and the EPEC protein Tir. Tir is inserted into the enterocyte plasma membrane (PM), resulting in the formation of actin-rich pedestals. Tir is translocated by the type III secretion system (TTSS), through a pore comprised of EPEC proteins inserted into the PM. Here, we demonstrate that in the absence of BFP, EPEC adherence, effector translocation and pedestal formation are dependent on lipid rafts. Lipid raft disruption using methyl-beta-cyclodextrin (MbetaCD) decreased adherence by an EPEC BFP-deficient strain from 85% to 1%. Translocation of the effectors Tir and EspF was blocked by MbetaCD treatment, although the TTSS pore still formed. MbetaCD treatment after Tir delivery decreased pedestal formation by EPEC from 40% to 5%, but not by the related pathogen E. coli O157:H7 which uses a different Tir-based mechanism. In contrast, EPEC expressing the BFP can circumvent the requirement for membrane cholesterol. This suggests that lipid rafts play a role in virulence of this medically important pathogen. | {
"pile_set_name": "PubMed Abstracts"
} |
The dose of 1-naphthaleneacetic acid determines flower-bud regeneration in tobacco explants at a large range of concentrations.
Short-term applications of very high concentrations of 1-naphthaleneacetic acid (NAA) to expiants from flower stalks of tobacco (Nicotiana tabacum L. cv. Samsun) induced flower-bud regeneration to the same extent as longer or continuous incubation on lower concentrations. The maximum number of flower buds per explant after 15 d of culture was obtained not only by continuous culturing at 1 μmol·l(-1) NAA but also by 12 h of culturing at 22 μmol·l(-1) or 0.5 h at 220 μmol· l(-1), followed by incubation on medium without auxin for the remaining period. Continuous application of such high concentrations resulted in callus formation or caused the death of the explanted tissue. In all experiments in which auxin concentration and time of application were independently varied, the product of concentration and time determined the number of buds formed. Most, but not all, of the NAA taken up by the tissues was converted into conjugates. In expiants which had received a dose which was optimal for regeneration, the internal concentration of free NAA remaining beyond the pulse period was between 1.7 and 6.2 μmol·l(-1). Suboptimal applications led to lower values, supraoptimal treatments to much higher internal concentrations. The physiological effect, which depends on the internal hormone concentration, thus manifested itself as dose-dependent with regard to applied hormone. | {
"pile_set_name": "PubMed Abstracts"
} |
Tilted birefringent Fabry-Perot etalon for tuning dye lasers.
A tilted birefringent Fabry-Perot etalon, which is set at an angle to the axis of the dye laser cavity and rotated about the normal of end-faces of the etalon, allows the dye laser emission to be tuned with very high finesse as obtained by two cascaded conventional Fabry-Perot etalons. | {
"pile_set_name": "PubMed Abstracts"
} |
Transsynaptic regulation of galanin, neurotensin, and substance P in the adrenal medulla: combinatorial control by second-messenger signaling pathways.
The adrenomedullary content of neurotensin and substance P was examined 1, 6, and 12 days after hypoglycemic shock. The neurotensin content was increased 60-fold within 24 h and remained elevated for up to 12 days, whereas the substance P content was increased approximately sevenfold within 24 h of insulin treatment and returned to control levels by 12 days poststimulation. Because protein kinase A, protein kinase C, and calcium influx in the rat adrenal medulla are all stimulated following splanchnic nerve stimulation, the differential regulation of neurotensin and substance P biosynthesis following stimulation of these three pathways was examined in bovine chromaffin cells in vitro. Neurotensin levels were up-regulated by elevated potassium, forskolin, and phorbol ester in bovine chromaffin cells. Substance P levels were up-regulated by elevated potassium and forskolin but not by phorbol ester treatment. When chromaffin cells were treated with phorbol ester in combination with forskolin, neurotensin levels were increased in a synergistic fashion, whereas phorbol ester antagonized the forskolin-induced elevation of substance P levels. Earlier, it was reported that galanin biosynthesis, like neurotensin biosynthesis, is upregulated by depolarization, phorbol ester stimulation, and forskolin treatment in chromaffin cells in vitro. Here we report that galanin is also, like neurotensin, increased greater than 60-fold after stimulation of the rat adrenal medulla in vivo. Neuropeptide-specific combinatorial effects of stimulating the calcium, protein kinase A, and protein kinase C signaling pathways may underlie the quantitative differences between galanin and neurotensin compared with substance P up-regulation in rat adrenal medulla after splanchnic nerve stimulation in vivo. | {
"pile_set_name": "PubMed Abstracts"
} |
[Disturbances of the clotting mechanism in severe cerebro-cranial injuries (author's transl)].
Factors II, V, X, VIII, thrombin time, platelet count and the thrombelastogram were estimated in 58 persons who had suffered cerebrocranial injuries of varying degree of severity. The aim of the investigation was to elucidate whether and to what extent the injury affected these parameters and whether the destruction of brain tissue could be regarded as the sole cause of the impairment of the clotting mechanism. First estimations in cases of cerebrocranial trauma uncomplicated by other injuries and in cases of cerebrocranial trauma complicated by moderately severe other injuries showed changes in factors II, V, X, VII as well as a pathological thrombelastogram, viz.: hypercoagulability. More pronounced changes were observed in cases where the cerebrocranial injuries were associated with severe complicating lesions: factors II, V, VII, X and the platelet count were reduced by 50-70 per cent of their normal values. Subsequent estimations carried out over a period of 15 days showed that the clotting mechanism had returned to nearly normal within 6-10 days. The use of heparin for normalizing disturbances of the clotting mechanism in cerebrocranial injuries is discussed. | {
"pile_set_name": "PubMed Abstracts"
} |
Examination of the transtheoretical model in current smokers.
Although the transtheoretical model of behavior change has frequently been used as a basisfor smoking-cessation programs, in very few studies have the study variables been linked with the theoretical concepts. This study used a convenience sample of 79 current smokers to determine the relationships among the stages of change and the processes of change. Logistic regressions were used in order to determine whether certain processes were related to specific stages and whether specific processes were related to movement among the stages. Participants in the precontemplation stage and the preparation stage were found to rely on specific processes, whereas those in the contemplation stage did not. | {
"pile_set_name": "PubMed Abstracts"
} |
Triple synchronous primary lung carcinomas treated with simultaneous resection.
The fifth case of surgical treatment for three primary bronchogenic lung cancers is presented. This is the second successful surgical case in which this occurred bilaterally and the first case in which simultaneous resection via median sternotomy for three separate primary bronchogenic carcinomas was performed. We strongly advocate this approach when feasible. | {
"pile_set_name": "PubMed Abstracts"
} |
Giving Voice to Values: an undergraduate nursing curriculum project.
Among the competency standards stipulated by the Australian Nursing and Midwifery Council for graduating students are competencies in moral and ethical decision making and ethics education within professions such as nursing has traditionally focussed on these competencies, on raising ethical awareness and developing skills of analysis and reasoning. However, ethics education in tertiary settings places less emphasis on developing students' capacities to act on their values. This paper explains and explores the adoption of Dr. Mary Gentile's curriculum (the Giving Voice to Values curriculum).which specifically focuses on developing students' capacities to act on their values. The curriculum (Gentile, 2010) assists students and professionals to explore, script and rehearse responses which build upon their capacity to respond in accordance with their own values in complex workplace settings in which they face conflicts of value and belief. The paper firstly examines the theoretical underpinnings of the Giving Voice to Values (GVV) curriculum. It then presents the integration and evaluation phase of a Project inspired by the GVV methodology, using a case study approach within two areas of an undergraduate nursing curriculum. As a pilot project, this initiative has provided signposts to further curriculum development and to research pathways within the UNDA School of Nursing, by highlighting students' uncertainties regarding their own professional values, and their intense struggles to voice their values within health care contexts. | {
"pile_set_name": "PubMed Abstracts"
} |
The D box asserts itself.
New findings (Burton et al., 2005; Kraft et al., 2005) demonstrate the direct recognition of D and KEN boxes, short sequence elements in substrates of the anaphase-promoting complex/cyclosome (APC/C), by APC/C coactivators and indicate a special role for the D box in the assembly of catalytically active APC/C. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of dietary N-acetylcysteine on the oxidative stress induced in tilapia (Oreochromis niloticus) exposed to a microcystin-producing cyanobacterial water bloom.
Fish can be exposed to toxic cyanobacterial cells in natural waters and fish farms and suffer from oxidative damage. The present study investigates the effects of N-acetylcysteine (NAC), a glutathione (GSH) precursor, on the oxidative stress induced by Microcystis cyanobacterial cells containing microcystins (MCs) in tilapia fish (Oreochromis niloticus). Variation in lipid peroxidation (LPO) levels, carbonyl group content, reduced glutathione to oxidized glutathione ratio (GSH:GSSG), and catalase (Enzyme Commission [EC] 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1), glutathione reductase (GR; EC 1.8.1.7), glutathione peroxidase (GPx; EC 1.11.1.9), and glutathione S-transferase (EC 2.5.1.18) activities in liver and kidney of tilapia exposed to a single oral dose of 120 microg MC-LR (with leucine [L] and arginine [R])/fish and killed in 24 h were investigated in the absence and presence of 20.0, 44.0, and 96.8 mg NAC/fish/d. Results showed a protective role of NAC, depending on the dose and the biomarker considered. The increase in LPO (1.9- and 1.4-fold in liver and kidney, respectively) and the decreased protein content and GSH: GSSG in the liver induced by MCs were recovered mainly by the lower doses of NAC employed. Antioxidant enzyme activities increased (range, 1.4- to 1.7-fold) by MCs also were ameliorated by NAC, although the highest level used induced significant alteration of some enzymatic activities, such as SOD, GPx, and GR. Thus, NAC can be considered to be a useful chemoprotectant that reduces hepatic and renal oxidative stress in the prophylaxis and treatment of MC-related intoxications in fish when careful attention is given to its application dose because of its own pro-oxidant activity, as shown in the present study at 96.8 mg NAC/fish/d. | {
"pile_set_name": "PubMed Abstracts"
} |
[Brief questionnaires for the early detection of alcoholism in primary health care].
The utility of two tests for the early detection of alcoholism (CBA and CAGE) is studied in a group of 247 adult patients of both sexes at the primary care environment. For the definition of the "problem drinker case", the Münchner alkoholismus test (MALT) protocol is used as "gold standard". A sensitiveness of 79 and 75% is obtained for the CBA and CAGE respectively, a specifity of 94 and 96%, and predictive positive value of 63 and 73%, with a prevalence of "problematic drinkers" of 12%. Both questionnaires are shown as efficient instruments for the secundary prevention of alcoholism in the primary care consulting rooms. | {
"pile_set_name": "PubMed Abstracts"
} |
Speech outcomes in patients rehabilitated with maxillary obturator prostheses after maxillectomy: a prospective study.
Speech outcome measurements are valuable in guiding treatment and determining the effectiveness of rehabilitation with a maxillary obturator prosthesis in individuals with palatal resection. Although speech outcome data exist in the literature for such patients, relatively few reports have used clinical tools designed to measure the acoustic, physiologic, and perceptual bases of speech. This investigation reports these measures for individuals rehabilitated with a maxillary obturator. Speech measurements were collected prospectively at three clinical visit times (preoperative, postresection without an obturator, and with a definitive obturator) for 12 patients assigned to three groups based on the extent of their resection (< half the hard palate, > or = half the hard palate, hard and soft palates). Acoustic data were obtained with the Nasometer, aeromechanical data were collected with the PERCI-SARS, and perceptual ratings of speech intelligibility were obtained through listener analysis. Significant differences existed among the three treatments for all dependent variables and revealed that speech without an obturator is significantly different from the preoperative state, while speech with an obturator does not differ significantly from preoperative function. Individuals with soft palate involvement exhibited significantly poorer nasalance values than individuals with involvement of the hard palate only. Rehabilitation with a maxillary obturator is successful in restoring preoperative speech function. Rehabilitation of individuals with involvement of the soft palate may be more challenging. | {
"pile_set_name": "PubMed Abstracts"
} |
Method validation and application of a selective multiresidue analysis of highly polar pesticides in food matrices using hydrophilic interaction liquid chromatography and mass spectrometry.
The effectiveness of highly polar pesticides in agriculture is well known, while their low costs contribute to the frequent use. On the other hand, their physicochemical properties make their analytical determination a challenging task. The aim of this study is the evaluation of a methanol-based extraction method with a simple clean-up step using a selective multiresidue LC-MS/MS method for 14 highly polar pesticides and their metabolites. For the clean-up step, several sorbents from different brands, with diverse mechanisms of action, were tested. Different dilution factors for the final extract were also evaluated in order to check the impact on the matrix effects. The optimised method was validated for matrices from different commodity groups. Recovery studies performed with grapes, lettuce, orange, oat and soya beans showed absolute average recoveries in the range 70-120% with relative standard deviation values below 20% for almost all the pesticides tested. The matrix effects observed were very different in each matrix and for each individual pesticide evaluated. Therefore, isotopically labeled procedural internal standards were used for all compounds in order to correct for recovery and matrix effects. Method Limits of Quantification for most analyte-matrix combinations were 0.02 or 0.05 mg kg-1. The final optimised method appeared to be reproducible and robust in routine analysis of a wide variety of fruits, vegetables and cereals. Monitoring results are presented to show the occurrence of the compounds studied in real samples. The residue concentrations ranged from 0.023 to 30 mg kg-1 for the analytes detected. | {
"pile_set_name": "PubMed Abstracts"
} |
Life Starts at Home: Bathing, Exertion and Participation for People With Mobility Impairment.
To investigate the relationship between perceived exertion while bathing/dressing/grooming and associations with social-recreational activities outside the home for individuals with mobility impairment (MI). A 2-study approach was used to examine data from the American Time Use Survey (ATUS) and primary data from the Health and Home Survey (HHS). The relationship between bathing/dressing/grooming and engagement in social-recreational activities was explored, as well as the role that exertion in the bathroom may play in participation in these activities. General community setting. For the ATUS survey, participants (n=6002) included individuals who reported an MI. For the HHS, 2 mail-based recruitment methods were used to recruit a sample of individuals with MI (n=170) across 3 geographically diverse U.S. communities. Not applicable. Participation in social and recreational activities. People with MI (relative to those without MI) were less likely to report spending any time bathing/dressing/grooming on a given day, but spent more time when they did. People with MI reported higher exertion while bathing/dressing/grooming than people without. People with MI were less likely to leave the house or engage in social-recreational activities on days where they did not engage in bathing activities. People who reported greater exertion in the bathroom engaged in fewer social-recreational activities. Exertion in the bathroom may present a barrier to participation, indicating a relationship between exertion in the bathroom and social-recreational participation. Research that examines the impact of home modifications on exertion and participation is needed. | {
"pile_set_name": "PubMed Abstracts"
} |
What is hidden in the biodiversity? The role of natural products and medicinal chemistry in the drug discovery process.
This manuscript describes the role of natural products in the process of drug discovery. In fact, several different natural compounds have been used as inspiration to develop new drugs. Some relevant examples are presented in chronological order. | {
"pile_set_name": "PubMed Abstracts"
} |
Development of multiple movement representations with practice: specificity versus flexibility.
The question addressed in the present experiment was whether an individual who practices a task under different conditions of afferent information develops different movement representations, each of which is based on the most accurate source of afferent information for movement control. In Experiment 1, participants (N = 23) performed a manual aiming movement in a target-only condition for 520 trials before performing in a normal vision condition for an equivalent amount of practice. Control groups performed all practice trials in either a normal vision or a target-only condition. The results revealed that the movement representation developed in the initial (target-only) practice phase remained accessible for movement planning and control. The results of Experiment 2 indicated, however, that participants did not maintain such a representation when their initial practice in the target-only condition was reduced (40 or 160 trials) before they had extensive practice in normal vision. Those results indicate that extensive practice in a target-only and then in a normal vision condition enables an individual to plan and control his or her movement on the basis of the most efficient source of available afferent information. Because visual afferent information provides optimal information for ensuring movement accuracy, however, if initial practice in the target-only condition is only modest or moderate it is likely that that information source will progressively dominate all other sources of afferent information for movement planning and control. | {
"pile_set_name": "PubMed Abstracts"
} |
Lightweight hydrogen-storage material Mg(0.65)Sc(0.35)D2 studied with 2H and 2H-{45Sc} MAS NMR exchange spectroscopy.
Using double-quantum (2)H MAS NMR with (45)Sc recoupling and Bloch-Siegert compensated (2)H-{(45)Sc} TRAPDOR we have identified the overlapping NMR signals of deuterium with and without scandium neighbors in Mg(0.65)Sc(0.35)D(2), a candidate lightweight material for hydrogen storage. At room temperature we also observe a third type of mobile deuterium. Deuterium mobility among the three NMR-distinct sites has been investigated by means of one-and two-dimensional exchange spectroscopy (Exsy). Complete deuterium exchange within 0.1s is observed, which indicates that the three NMR-distinct sites are close together in the crystal lattice. The weak temperature- and MAS-rate dependences observed in Exsy are indicative for a combination of chemical exchange and spin diffusion. | {
"pile_set_name": "PubMed Abstracts"
} |
The role of the French community pharmacist in drug dispensing.
Pharmacists are in charge of decreasing the risk of drug related morbidity and then enhance the quality of drug therapy. A survey of the optimization of pharmacotherapy carried out by 37 pharmacists during six weeks. When dispensing drugs, pharmacists have detected drug related problems, such as lack of precision in prescriptions, abnormalities in dosing, interactions, contraindications, adverse drug events or noncompliance. Among 727 reported cases, 45% led to a major change in the prescription after contact with the physician. These results confirm that a close cooperation between physicians and pharmacists is essential for the safety and the efficiency of pharmacotherapy. | {
"pile_set_name": "PubMed Abstracts"
} |
HIV type 1 intraperitoneal infection of rabbits permits early detection of serum antibodies to Gag, Pol, and Env proteins, neutralizing antibodies, and proviral DNA from peripheral blood mononuclear cells.
The aim of this study is the development of an animal model useful for studying HIV-1 pathogenesis, candidate vaccines, and antiviral drugs. Aseptic thioglycolate peritonitis was induced in six rabbits. After 4 days, four rabbits were infected with 1 ml of HIV-1 stock containing 100 times the MID50. Blood samples were collected every 2 weeks for 8 months. Serum antibodies were tested by ELISA, using as antigen the recombinant protein p24; synthetic peptides of highly conserved regions of p31, gp41, and gp120; and a synthetic peptide of gp120 at the V3 loop region of HIV-1 strains IIIB and MN. Furthermore, neutralizing antibodies were tested by a microscale neutralization assay. Proviral DNA was detected by PCR, and virus isolation was performed by a cocultivation technique using primary rabbit peripheral blood mononuclear cells (PBMCs). All infected rabbits produced antibodies to HIV-1 proteins within 2 weeks and up to 8 months after virus infection. Serum antibodies were directed against the Env (gp120 and gp41), Gag (p24), and Pol (p31) proteins and against two synthetic peptides whose sequence corresponds to gp120 at the V3 loop region of HIV-1 strains IIIB and MN. Neutralizing antibodies were also detected in the sera of infected animals. Proviral DNA was detected in PBMCs by PCR within 4 weeks and up to 8 months after HIV-1 infection. HIV-1 was also isolated from PBMCs of infected animals at 30, 60, and 120 days after infection. Results obtained indicate that HIV-1 intraperitoneal infection of the rabbit permits the early detection of serum antibodies to Gag, Pol, and Env proteins, neutralizing antibodies, and proviral DNA sequences from PBMCs. | {
"pile_set_name": "PubMed Abstracts"
} |
[Johannes Trüper--mediator between child and adolescent psychiatry and pedagogy].
The development of the child and adolescent psychiatry as an independent speciality is not only a result of sub-specialisation within medicine or psychiatry but also a result of interaction with pedagogy, psychology, and philosophy. Within this framework, pedagogy (and more specifically orthopedagogy) has played an essential scientific role. This will be demonstrated by the work of Johannes Trüper. A short description of his life and an evaluation of his scientific work is presented based on earlier publications and archive studies. Trüper founded a famous approved school on Sophienhöhe close to Jena in 1892 and was a co-founder of "Die Kinderfehler" (1896), one of the leading journals for research in pedagogy and child psychiatry in its time. The psychiatrist and philosopher Theodor Ziehen regarded as one of the pioneers of child psychiatry, gained practical child psychiatric experience as a consultant liaison psychiatrist at the approved school which was run by Johannes Trüper. Wilhelm Strohmayer, another psychiatrist from Jena, also belongs to the founding fathers of child psychiatry in Germany with his book "Vorlesungen uber die Psychopathologie des Kindesalters für Mediziner und Pädagogen" (1910) which is based on his consultant work on Sophienhöhe. The close and direct collaboration between psychiatry and pedagogy, which lead to an early development of child psychiatry as a speciality in Jena, can be regarded as a particularity in the scientific development. | {
"pile_set_name": "PubMed Abstracts"
} |
Autocrine role of macrophage inflammatory protein-1 beta in human T-cell lymphotropic virus type-I tax-transfected Jurkat T-cells.
The interaction of human T-cell lymphotropic virus I (HTLV-I) tax gene with host cell factors plays an important role in the maintenance of the transformed state. There have been numerous reports that have demonstrated the role of tax in transactivating several cytokines. In this study, we show that upon mitogen stimulation, macrophage inflammatory protein-1 beta (MIP-1beta) is expressed and secreted in tax-transfected Jurkat cells. Furthermore, expression of CC-chemokine receptor-5 (CCR5) mRNA in these cells suggests an autocrine role for MIP-1beta in HTLV-I-infected T-cells. These results, coupled with our earlier observations, demonstrate the influence that extracellular Tax protein might have on modulating host chemokines for proliferation and transformation of uninfected cells. | {
"pile_set_name": "PubMed Abstracts"
} |
If F(ST) does not measure neutral genetic differentiation, then comparing it with Q(ST) is misleading. Or is it?
The comparison between neutral genetic differentiation (F(ST) ) and quantitative genetic differentiation (Q(ST) ) is commonly used to test for signatures of selection in population divergence. However, there is an ongoing discussion about what F(ST) actually measures, even resulting in some alternative metrics to express neutral genetic differentiation. If there is a problem with F(ST) , this could have repercussions for its comparison with Q(ST) as well. We show that as the mutation rate of the neutral marker increases, F(ST) decreases: a higher within-population heterozygosity (He) yields a lower F(ST) value. However, the same is true for Q(ST) : a higher mutation rate for the underlying QTL also results in a lower Q(ST) estimate. The effect of mutation rate is equivalent in Q(ST) and F(ST) . Hence, the comparison between Q(ST) and F(ST) remains valid, if one uses neutral markers whose mutation rates are not too high compared to those of quantitative traits. Usage of highly variable neutral markers such as hypervariable microsatellites can lead to serious biases and the incorrect inference that divergent selection has acted on populations. Much of the discussion on F(ST) seems to stem from the misunderstanding that it measures the differentiation of populations, whereas it actually measures the fixation of alleles. In their capacity as measures of population differentiation, Hedrick's G'(ST) and Jost's D reach their maximum value of 1 when populations do not share alleles even when there remains variation within populations, which invalidates them for comparisons with Q(ST) . | {
"pile_set_name": "PubMed Abstracts"
} |
Anti-adalimumab antibodies and adalimumab concentrations in psoriatic arthritis; an association with disease activity at 28 and 52 weeks of follow-up.
To investigate the relationship between antidrug antibodies (ADA), adalimumab concentrations and clinical response in patients with psoriatic arthritis (PsA) during 52 weeks of follow-up. This prospective cohort study included 103 consecutive patients with PsA. Disease Activity Score of 28 joints (DAS28), Erythrocyte Sedimentation Rate, C reactive protein and Psoriasis Area and Severity Index were assessed. Adalimumab concentrations and ADA were measured in serum trough samples, using an ELISA and a radio immunoassay, respectively. Adalimumab concentrations were significantly lower at 28 and 52 weeks in patients with detectable ADA compared with patients without detectable ADA (at week 28: 1.3 mg/L (IQR 0.0-3.2) versus 8.7 mg/L (IQR 5.7-11.5), p<0.001; at week 52: 0.9 mg/L (IQR 0.0-2.9) vs 9.4 mg/L (IQR 5.7-12.1), p=0.0001). DAS28 at 28 weeks (2.16 vs 2.95, p=0.023) and 52 weeks (2.19 vs 2.95, p=0.024) showed a significant difference; patients with detectable ADA had a poorer clinical outcome than patients without. Patients with detectable ADA had lower adalimumab concentrations and a significantly poorer clinical outcome compared with patients in whom ADA were not detected. | {
"pile_set_name": "PubMed Abstracts"
} |
National Quality Program Achieves Improvements in Safety Culture and Reduction in Preventable Harms in Community Hospitals.
Through an innovative affiliation, Duke University Health System (DUHS), a large and complex academic health system, and LifePoint Health® (LifePoint [LP]) collaborated to create a joint venture, DLP Healthcare (DLP) to measurably improve culture and quality and patient safety metrics in community hospitals across the United States. A structured approach to quality was developed in DLP hospitals and later refined and spread to all LP hospitals through the National Quality Program (NQP). The NQP was designed to drive organizationwide performance improvement through use of a framework of leadership, performance improvement, and culture. A comprehensive quality assessment of each DLP and LP hospital led to the creation of a customized improvement plan that was specific to the performance level of individual hospitals and aligned with strategic organizational goals. The improvement process was data driven, managed with defined improvement methodologies and practices, and implemented in a culture that honors teamwork, mutual respect, accountability and provider well-being. Implementation of the NQP has led to significant improvements in patient safety metrics and in safety culture, which have now been sustained for more than seven years. Aggregate harm, as measured by administrative claims data-based harms per 1,000 inpatient-days, was reduced by 62.5% between January 2011 and December 2017, as compared to 2010 baseline data. The LP and Duke journey to achieve high reliability in community hospitals has yielded significant improvement in measures of patient safety and culture. The results are consistent with literature supporting the link between culture and overall performance. | {
"pile_set_name": "PubMed Abstracts"
} |
Inhibitory action on GHRH-induced GH secretion of chronic tamoxifen treatment in breast cancer.
Previous in vitro and in vivo studies on animal models have demonstrated that tamoxifen (TAM) inhibits GH secretion. Studies in humans are conflicting. The aim of this study was to evaluate the effect of chronic TAM treatment on GH secretory dynamics in the presence of negligible endogenous oestrogens, in postmenopausal women with breast cancer. Ten female patients were studied over a 6-12-month period after surgical therapy, before medical therapy, and during chronic treatment with TAM (20 mg/day p.o.). In all subjects we performed a standard GHRH-test (50 mg i.v. as a bolus) and compared the single time points, the peak response and the areas under the curves (AUC), before and during treatment. In basal samples, we evaluated the circulating levels of IGF-1, IGF-BP3 and their ratio, SHBG, FSH, LH, Oestradiol (E2) and PRL. GH was assayed by Immunoradiometric assay (IRMA). Insulin-like growth factor type I (IGF-I), Insulin-like growth factor-binding protein-3 (IGF-BP3), FSH, LH and PRL were measured by Radioimmunoassay (RIA). SHBG was measured by a noncompetitive liquid phase immunoradiometric assay, while E2 was measured directly in plasma by a liquid phase technique. TAM chronic treatment significantly reduced GH response to GHRH at single time point evaluations, GH peak response (mean decrease: 59.8 ± 7.3%) and GH-AUC (mean decrease 53.8 ± 8.9%). TAM also significantly reduced plasma IGF-1 levels. No significant variations were found in IGF-BP3 levels or in the IGF-1/IGF-BP3 ratio. A significant inverse correlation between SHBG and IGF-1 circulating levels was noticed during TAM treatment. Our data show that long-term tamoxifen treatment blocks the response of GH to exogenous GHRH and reduces IGF-1 levels, possibly by a central mechanism other than the demonstrated peripheral action. The results of this study, keeping in mind the demonstrated mitogenic role of IGF-1 in cancer proliferation, can contribute to clarify the mechanism by which TAM exerts its antiproliferative effect. | {
"pile_set_name": "PubMed Abstracts"
} |
Mycobacterium chelonae-Mycobacterium abscessus complex clear corneal wound infection with recurrent hypopyon and perforation after phacoemulsification and intraocular lens implantation.
We report a clear corneal wound infection occurring in a 74-year-old man caused by a member of the Mycobacterium chelonae-Mycobacterium abscessus complex, presenting as crystalline keratopathy with recurrent hypopyon. This led to perforation after phacoemulsification with posterior chamber intraocular lens implantation. Only after corneal biopsy of the incision was the causative organism isolated and found to be sensitive to clarithromycin and ciprofloxacin. Despite aggressive therapy, a full-thickness corneal perforation developed, requiring emergent cyanoacrylate glue to preserve ocular integrity. Both the difficulty and delays in obtaining a correct diagnosis led to severe ocular morbidity. Infectious lamellar keratitis limited to the clear cornea phacoemulsification incision is rare, but some unusual organisms such as atypical mycobacteria may be encountered. | {
"pile_set_name": "PubMed Abstracts"
} |
Temperature-induced unfolding pathway of a type III antifreeze protein: insight from molecular dynamics simulation.
Molecular dynamics simulations of the temperature-induced unfolding reaction of a cold-adapted type III antifreeze protein (AFPIII) from the Antarctic eelpout Lycodichthys dearborni have been carried out for 10 ns each at five different temperatures. While the overall character and order of events in the unfolding process are well conserved across temperatures, there are substantial differences in the timescales over which these events take place. Plots of backbone root mean square deviation (RMSD) against radius of gyration (Rg) serve as phase space trajectories. These plots also indicate that the protein unfolds without many detectable intermediates suggestive of two-state unfolding kinetics. The transition state structures are identified from essential dynamics, which utilizes a principal component analysis (PCA) on the atomic fluctuations throughout the simulation. Overall, the transition state resembles an expanded native state with the loss of the three 3(10) helices and disrupted C-terminal region. Our study provides insight into the structure-stability relationship of AFPIII, which may help to engineer AFPs with increased thermal stability that is more desirable than natural AFPs for some industrial and biomedical purposes. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of atriopeptin and chicken heart extract in Gallus domesticus.
Effects of Ser-Leu-Arg-Arg-atriopeptin III (ANP) and chicken heart extract (CHE) were compared during unilateral renal portal infusion in anesthetized Single Comb White Leghorn chickens. The purpose was to determine whether renal effects were glomerular and/or tubular. Both CHE and ANP caused substantial decreases in mean arterial pressure but had different renal actions. ANP caused small but significant increases in both absolute and fractional sodium excretion, but these effects were modest compared with those reported in mammals. Although there was a tendency for higher fractional sodium excretion in the portal infused kidney, the difference was not significant (0.1 less than P greater than 0.05). ANP also increased glomerular filtration rate (GFR), urine flow rate (UFR), and osmolal clearance and decreased estimated renal vascular resistance. In contrast, CHE decreased GFR and increased resistance. In contrast, CHE decreased GFR and increased fractional potassium excretion in the infused kidney. After CHE infusion was stopped, GFR and UFR increased, and there was a further transient kaliuresis. No natriuretic effects were ever seen with CHE. Chickens apparently lack potent mammalian-type cardiac natriuretic factor(s) and/or have a limited capacity for natriuresis in response to mammalian ANP. Because hypotension was the most prominent avian response to both CHE and ANP, endogenous vasoactive factor(s) in chicken hearts may function to regulate blood pressure rather than blood volume. | {
"pile_set_name": "PubMed Abstracts"
} |
Possible association between 3,4-methylenedioxymethamphetamine abuse and valvular heart disease.
Valvular heart disease, inducing valvular regurgitation, has been described in users of drugs such as anorectic agents and ergot derivates. 3,4-Methylenedioxymethamphetamine (MDMA; "ecstasy") also leads in vitro to the proliferation of cardiac valvular interstitial cells by activation of the 5-hydroxytryptamine 2B receptor. The aim of this study was to determine the occurrence of valvulopathy in young adults taking MDMA. Twenty-nine subjects using or having used MDMA and 29 gender- and age-matched controls were blindly evaluated with echocardiography. Eight subjects (28%) who took MDMA had abnormal echocardiographic results using the United States Food and Drug Administration's criteria for appetite suppressant-induced valvular heart disease, compared with none in the control group (p = 0.0045). Six (21%) subjects had mitral regurgitation of 1/4 and 4 (14%) of > or =2/4, compared with none in the control group (p = 0.002). The mean mitral regurgitant area ratios (jet/atrium) were 12 +/- 9.8% and 5 +/- 1.3%, respectively (p = 0.007). Tricuspid regurgitation > or =2/4 was present in 13 MDMA users (45%) and absent in controls (p <0.001). The mean tricuspid regurgitant area ratios were 19 +/- 9.5% and 9 +/- 4.5%, respectively (p <0.001). Four MDMA users (14%) had mild aortic regurgitation (p = 0.11). Valvular "strands" were present in 6 MDMA users (21%) and in none of the controls (p = 0.02). In conclusion, MDMA may lead to mild to moderate valvular heart disease and valvular strands. | {
"pile_set_name": "PubMed Abstracts"
} |
Mente Activa® Improves Impaired Spatial Memory in Aging Rats.
Aging is accompanied by a decline in several aspects of the cognitive function, having negative personal and socioeconomic impacts. Dietary supplements could be beneficial for preventing age-related cognitive decline. In this context, we examined whether the nutritional supplement Mente Activa® has beneficial effects on aging-related cognitive deficits without inducing side effects. Mente Activa® was administered to old rats (n= 30 treated rats and n= 30 control rats) during 5 months, and the Morris water maze was used to test the learning capacities of the animals. The first assessment was conducted before the nutritional intervention (age of 18-19 months), to determine the baseline of the performance of animals on this test, and the second assessment was performed at the end of the treatment (23-24 moths). In order to examine possible secondary effects of this nutritional supplement, plasma, heart anatomy and liver parameters were evaluated. Our data indicate that supplemented rats showed less escape latency, distance swum, higher use of spatial search strategies, and crossed the former platform location with higher frequency than control rats. These effects were specific of the treatment, indicating that this nutritional supplement has a beneficial effect on spatial memory. On the other hand, the regular intake of Mente Activa® did not induce any negative effects in plasma parameters and heart size. Aged rats under a sustained dietary intake of the nutritional supplement Mente Activa® displayed improved learning and memory abilities compared to the non-treated rats. These results suggest the therapeutic potential and safety of use of Mente Activa® for age-related cognitive deficits, particularly, in the onset of the first cognitive dysfunction symptoms. | {
"pile_set_name": "PubMed Abstracts"
} |
Hamstring Tendon Regeneration After Harvest for Anterior Cruciate Ligament Reconstruction: A Systematic Review.
To assess whether the portions of the semitendinosus and gracilis tendons harvested for anterior cruciate ligament reconstruction have the potential to regenerate and, if so, to evaluate the histologic properties and actual function of this newly formed tissue. We performed a comprehensive search of CINAHL (Cumulative Index to Nursing and Allied Health Literature), Embase, Medline, the Cochrane Central Registry of Controlled Trials, and SPORTDiscus from inception of the databases to July 2014, using various combinations of keywords. Studies focusing on hamstring tendon and muscle regeneration through imaging and histology, as well as on the related functional outcomes, were selected. We included studies assessing evidence of tissue regeneration with imaging (magnetic resonance imaging, 3-dimensional computed tomography, ultrasonography) or with histologic examination of biopsy samples (or a combination thereof). Nineteen articles were included in this review, with a total of 400 patients observed. The overall rate of tissue regeneration was 86.0%, with similar values shown in most studies regardless of the methodology of the assessment. Biopsy confirmed that the tissue found at the site in 74% of the cases showed typical histologic features of the tendon. The mean modified Coleman Methodology Score of the studies included was 52.7 points, showing a modest methodologic quality for the studies published to date. In over 85% of the cases analyzed, regeneration signs of the harvested tendon were found through different imaging and histologic methodologies. A torque deficit in deep knee flexion is always present postoperatively, but the cause for this is still unclear. There is a need for better-designed trials featuring a higher level of evidence to further investigate this matter, and the effects of postoperative care and the surgical approach used on the regeneration process should be analyzed in the future. Level IV, systematic review of Level II, III, and IV studies. | {
"pile_set_name": "PubMed Abstracts"
} |
[Lesions of the femoral nerve during the gynaecological operations (author's transl)].
The Authors present two cases of unilateral and bilateral lesions of the femoral nerve and emphasize the frequency, the characteristics, the mechanisms and the course of such lesions. | {
"pile_set_name": "PubMed Abstracts"
} |
Properties of allelic variants of phosphoglucomutase from the sea anemone Metridium senile.
The phosphoglucomutase (Pgm) locus from populations of the sea anemone Metridium senile has three alleles in natural populations from the northeastern coast of North America. Two of the alleles exhibit clinal variation north of Cape Cod, suggesting a possible association of allele frequency with environmental temperature. This clinal pattern is reproducible and stable over at least brief periods of time. The allozymes encoded by each of the six Pgm genotypes have been partially purified and characterized. The symmetrical pH optimum for Vmax is pH 7.5; the apparent Km (Kmapp) of glucose-1-phosphate declines monotonically as the pH increases from 6.5 to 8.5. There are no pronounced differences in heat stabilities of PGM produced by various genotypes, nor are there significant differences in specific activities. There are no differences in the sensitivity of Vmax to temperature. Kmapp values are very low for all genotypes, ranging from about 2 to 12 microM, depending upon the temperature. Kmapp of glucose-1-phosphate declines as the temperature is raised for all genotypes, whether the pH is held constant or allowed to vary with the temperature. Under certain conditions, there are small significant differences among genotypes in Kappm values, but there is no systematic pattern to these differences. The present data provide no biochemical explanation for the maintenance of the Pgm cline by selection for functional differences under different thermal regimes. | {
"pile_set_name": "PubMed Abstracts"
} |
Detection of HPV types and neutralizing antibodies in Gansu province, China.
A total of 82 samples from patients with cervical cancer (Group 1) and 50 samples from patients with other genital diseases (Group 2) were collected in Gansu, China. All 132 samples were tested for HPV DNA with a typing kit that can detect 21 types of HPV, and also tested for neutralizing antibodies against HPV-16, -18, -58, -45, -6, and -11 using pseudovirus-based neutralization assays. The results revealed that 28% (23/82) of sera in Group 1 were positive for type-specific neutralizing antibodies with a titer range of 160-640, of which 23.2% (19/82), 2.4% (2/82), 2.4% (2/82), 1.2% (1/82), and 1.2% (1/82) were against HPV-16, -58, -6, -18, and -45, respectively. Only one serum (2%) in Group 2 was positive for neutralizing antibodies, which were against HPV-6 with a titer of 2,560. Overall, 85.4% (70/82) of samples in Group 1 were HPV DNA-positive, compared with 28% (14/50) of samples in Group 2. The seven most common types detected in Group 1 were HPV-16 (80%), HPV-52 (7.1%), HPV-66 and HPV-11 (5.7% each), and HPV-58, HPV-18, and HPV-33 (4.3% each), while the four most common types in Group 2 were HPV-16 (12%), HPV-52 and HPV-11 (6% each), and HPV-68 (4%). The concordance between HPV DNA and corresponding neutralizing antibodies was 32.9% (27/82) with a significant difference (P < 0.005). More specifically, the concordance was 42.7% (35/82) for HPV-16 in Group 1. The full-length sequences of six HPV types (HPV-16, -58, -33, -59, -11, and -68) were determined and showed 99% identities with their reported genomes. | {
"pile_set_name": "PubMed Abstracts"
} |
Cost analysis of transthoracic cardioversion of atrial fibrillation with and without ibutilide pretreatment.
Ibutilide may result in chemical cardioversion of atrial fibrillation and facilitates transthoracic cardioversion by lowering the defibrillation energy requirement. Whether routine pretreatment with ibutilide increases or decreases the cost of cardioversion is unknown. The purpose of this study was to compare the cost of outpatient transthoracic cardioversion of atrial fibrillation with and without ibutilide pretreatment. Using a model based on published literature and hospital accounting information, a hypothetical group of 100 patients with atrial fibrillation and a left ventricular ejection fraction >0.30 underwent 2 strategies of outpatient cardioversion: transthoracic cardioversion with and without routine pretreatment with 1 mg ibutilide, and with and without involvement of an anesthesiologist for sedation. If transthoracic cardioversion was unsuccessful in patients who did not receive ibutilide, transthoracic cardioversion was repeated after administration of ibutilide. If an anesthesiologist was involved, transthoracic cardioversion with ibutilide was associated with incremental cost-savings as the efficacy of ibutilide alone in restoring sinus rhythm increased above the critical values of 20%, 27%, and 35% when the efficacy of transthoracic cardioversion alone was 60%, 80%, and 100%, respectively. In the absence of an anesthesiologist, routine pretreatment with ibutilide increased the cost of cardioversion at all success rates of transthoracic cardioversion. In the presence of an anesthesiologist, whether or not routine pretreatment with ibutilide lowers the mean cost of cardioversion is determined by the success rates of chemical cardioversion with ibutilide and transthoracic cardioversion. In the absence of an anesthesiologist, ibutilide pretreatment increases the cost of cardioversion. | {
"pile_set_name": "PubMed Abstracts"
} |
Buffalo meat as a salted and cured product.
Bresaola, a GPI product, is produced by salting and curing different cuts of the hindquarters of lean bovine meat. The use of buffalo meat to produce similar processed, cold-cut products demonstrated the possibility of transforming cuts of buffalo rump into a product similar to that of beef, even considering parameters that penalise the buffalo product like tenderness and colour. | {
"pile_set_name": "PubMed Abstracts"
} |
An evaluation of the use of databases in protein structure refinement.
The speed of electron-density fitting during X-ray structure solution and refinement, and the quality of the protein model resulting, can both be enhanced by the use of databases of main- and side-chain conformations. Three structures are compared in this report, one refined at high resolution (1.7 A), and two at lower resolutions using either the database method (2.4 A resolution) or more traditional empirical electron-density fitting (1.9 A resolution). An analysis of peptide orientation was used as an aid in finding unusual portions of main-chain structure. The fit of side chains to known rotamer conformations was used to help determine the accuracy of these atomic positions. In addition, the use of an objective measure of the fit of structures to electron-density maps was evaluated, both alone and in combination with side-chain conformational information. | {
"pile_set_name": "PubMed Abstracts"
} |
The induction of reparative dentine by enamel proteins.
This study was designed to examine whether enamel matrix derivative (EMD) could induce reparative dentine formation without eliciting adverse side-effects in pulpotomized teeth in the miniature swine. Pulpotomy was performed in 36 mandibular incisor teeth from 11 adult miniature swine. Following the surgical procedure, the exposed pulp tissue was treated with EMD or covered with a calcium hydroxide preparation (Dycal). Following an observation period of 3, 4 and 8 weeks, the experimental teeth were extracted and examined using light microscopy and histometric analysis. The total amount of reparative dentine formed in the EMD-treated teeth was calculated as total area using digital histomorphometry analysis of the five central-most sections from each experimental tooth. In the EMD-treated teeth, substantial amounts of dentine-like tissue formation consistently led to a complete hard-tissue bridging of the defects. The onset of hard tissue formation could be observed after 2 weeks and was located only on the pulpal wound. More limited dentine formation was also observed in Dycal-treated teeth. However, in these teeth the new hard tissue formed at the expense of pulp chamber width, causing narrowing of root canals. The total amount of reparative dentine formed in the EMD-treated teeth was significantly higher (P<0.005) than in the Dycal-treated specimens. These results demonstrate the potential of EMD as a biologically active pulp-dressing agent that specifically induces pulpal wound healing and dentine formation in the pulpotomized teeth without affecting the normal function of the remaining pulp. | {
"pile_set_name": "PubMed Abstracts"
} |
[Quarterly occurrence of selected types of birth defects in the Czech Republic in the 1994-2005 twelve-year period].
An analysis of quarterly occurrence of selected types of birth defects in the Czech republic in the 1994 - 2005 twelve-year period. Evaluation of possible changes of occurrence of selected types of birth defects along with standard deviations from mean values calculated from 1974 - 1993 period data. Retrospective analysis of quarterly data from the National Birth Defect Register in 1994 - 2005 period. Mean incidences of birth defects from the previous 1974-2003 period (per 10,000 live births) were calculated and compared to current data from each particular quarter. A calculation of observed/expected rates (O/E ratio) for particular selected birth defects was performed. Further, results are presented by means of figures and are widely discussed. In 1994-2005 period, totally 1,132,567 children were born in the Czech Republic. Out of this number, 38 298 children were born with one or more birth defects. The mean incidence of birth defects was 338.15 per 10,000 live birth in the period under the study. Our results make clear, that incidences of particular defects as well as incidences as a whole are influenced by several factors making possible incidences increase. These factors are extremely difficult to assess individually and separately, therafore a particular period and a particular population is to be considered. Incidences of 11 selected types of birth defects and their time-changes are analyzed. Current registration of birth defects and their incidences analysis as a vital part of a environmental factors assessment is underlined. | {
"pile_set_name": "PubMed Abstracts"
} |
[In vitro Antiviral Activity of Recombinant Antibodies of IgG and IgA Isotypes to Hemagglutinin of the Influenza A Virus].
Seasonal and highly infectious strains of the influenza A and influenza B viruses cause millions of cases of severe complications in elderly people, children, and patients with immune diseases each year. Immunoglobulin A (IgA), which is an active component of humoral immunity, can prevent the spread of the virus in the upper respiratory tract. The preparation and study of the properties of recombinant virus-specific IgA could be an important approach to finding new means of preventing and treating influenza. Based on CHO DG44 cells, we developed stable monoclonal cell lines that produce monomeric and dimeric antibodies FI6-IgA1 and FI6-IgA2m1 to hemagglutinin (HA) of the influenza A virus. When studying the productivity, growth, and stability of the obtained clones, we found that the dimeric form of antibodies of IgA1 isotype is superior to other forms. The dimeric form of IgA antibodies plays a key role in mucosal immunity. Recognizing the prospects of using dimeric IgA as prophylactic and therapeutic mucosal drugs for viral infections, we studied their virus-neutralizing and antiviral activities on MDCK cell culture and compared them with the antibodies of the IgG1 isotype. This study presents the data on antiviral and virus-neutralizing activities of the FI6-IgA1 dimers to seasonal and highly infectious strains of influenza A virus. | {
"pile_set_name": "PubMed Abstracts"
} |
Experimental design for kinetic analysis of protein-protein interactions with surface plasmon resonance biosensors.
The reaction between antibody immobilized to surfaces, with and without a dextran matrix, and antigen in solution was studied using surface plasmon resonance detection. The use of a reference surface made it possible to identify conditions where a response related to changes in matrix conformation could be ignored. It was possible therefore to improve data quality by separating signals related to binding events from signals due to differences in refractive index between sample and running buffer. When antigen was injected over antibody immobilized to surfaces with and without dextran matrix the binding curves were virtually superimposable. Consequently, no binding artifacts associated with the dextran matrix were observed. Sets of binding curves obtained with different antigen concentrations were analyzed using numerical integration of differential rate equations and global fitting. When data was inconsistent with a one to one reaction it was possible to obtain good fits to an entire data set assuming several other reaction schemes including parallel, competitive and two-state reactions. Thus data analysis alone was not sufficient to discriminate between different reaction schemes. In contrast several reaction schemes could be ruled out with simple experiments; the duration of antigen injection, and reanalysis of antigen recovered in fractions from the antibody surface. In view of these findings experimental design appears to be the key to successful interaction analysis. | {
"pile_set_name": "PubMed Abstracts"
} |
Measurement of peripheral tissue thickness by ultrasound during the perioperative period.
We have studied changes in peripheral tissue thickness with a novel hand-held ultrasound device during the perioperative course of 60 healthy surgical patients in three different intraoperative body positions. The nil-by-mouth period led to a significant decrease in forehead tissue thickness. Standardized infusion therapy with Ringer's solution at a rate of 8 ml kg-1 h-1 resulted in a gradual increase in tissue thickness, which was significantly different from preoperative baseline values after 90 min. Packed cell volume decreased significantly after the start of infusion and remained low over the rest of the observation time. Different body positions did not influence changes in tissue thickness. We conclude that changes in perioperative tissue thickness in healthy patients can be detected easily by ultrasound, independent of body position. This method may prove useful for the non-invasive assessment of fluid balance state. | {
"pile_set_name": "PubMed Abstracts"
} |
Validation of an accelerometer for measuring sport performance.
Weightlifting technique is a well-studied subject with regard to standard biomechanical analysis that includes barbell velocity as well as barbell trajectory, but kinematic data such as barbell acceleration have not often been reported. Real-time or near-real-time feedback can be more helpful to coaches and athletes than delayed feedback. The purpose of this study was to validate measures obtained by a commercially available accelerometer in comparison with kinematic data derived from video. The hypothesis was that there would be a high positive relationship between accelerometer data and acceleration measures derived from video records of a barbell high-pull movement. Accelerometer values and kinematic data from high-speed video were obtained from 7 volunteers performing 2 trials each of a barbell high-pull. The results showed that the accelerometer measures were highly correlated with derived acceleration data from video (r = 0.94-0.99). On the basis of these results, the device was considered to be validated; thus, the unit may be a useful tool to measure acceleration during real-time training sessions rather than only reserved for collecting data in a laboratory setting. This device can be a valuable tool to provide instant feedback to coaches and athletes to assess individual barbell acceleration performance. | {
"pile_set_name": "PubMed Abstracts"
} |
Conversion from laparoscopic to open cholecystectomy: multivariate analysis of preoperative risk factors.
Laparoscopic cholecystectomy has become the gold standard in the treatment of symptomatic cholelithiasis. Some patients require conversion to open surgery and several preoperative variables have been identified as risk factors that are helpful in predicting the probability of conversion. However, there is a need to devise a risk-scoring system based on the identified risk factors to (a) predict the risk of conversion preoperatively for selected patients, (b) prepare the patient psychologically, (c) arrange operating schedules accordingly, and (d) minimize the procedure-related cost and help overcome financial constraints, which is a significant problem in developing countries. This study was aimed to evaluate preoperative risk factors for conversion from laparoscopic to open cholecystectomy in our setting. A case control study of patients who underwent laparoscopic surgery from January 1997 to December 2001 was conducted at the Aga Khan University Hospital, Karachi, Pakistan. All those patients who were converted to open surgery (n = 73) were enrolled as cases. Two controls who had successful laparoscopic surgery (n = 146) were matched with each case for operating surgeon and closest date of surgery. Descriptive statistics were computed and, univariate and multivariate analysis was done through multiple logistic regression. The final multivariate model identified two risk factors for conversion: ultrasonographic signs of inflammation (adjusted odds ratio [aOR] = 8.5; 95% confidence interval [CI]: 3.3, 21.9) and age > 60 years (aOR = 8.1; 95% CI: 2.9, 22.2) after adjusting for physical signs, alkaline phosphatase and BMI levels. Preoperative risk factors evaluated by the present study confirm the likelihood of conversion. Recognition of these factors is important for understanding the characteristics of patients at a higher risk of conversion. | {
"pile_set_name": "PubMed Abstracts"
} |
Characterization of proanthocyanidins in grape seeds using electrospray mass spectrometry.
Two proanthocyanidin (PA) fractions, one (Sdp3) with the mean degree of polymerization (mDP) of 3 and the other (Sdp9) with mDP of 9, were obtained from a Vitis vinifera cv. Shiraz grape seed extract. The PA fractions were directly analyzed by electrospray ionization mass spectrometry (ES-MS) and negative ion mass spectra were recorded. The mass spectrum of Sdp3 exhibited only singly charged ions corresponding to the molecular mass of PA with a degree of polymerization (DP) up to 9 (nonamers). In contrast, Sdp9 yielded rather complex mass spectra featuring ions with single [M - H](-), double [M - 2H](2-) and triple [M - 3H](3-) charge representing the molecular masses of PAs up to a DP of 28. In addition, the degree of galloylation per procyanidin (DG) was observed to be up to 5 (pentagallates) in Sdp3 and 8 (octagallates) in the Sdp9. This is the first evidence obtained by mass spectrometry for the distribution of grape seed PAs with such a high degree of polymerization and a broad diversity of galloylation. ES-MS data together with the complementary information provided by acid hydrolysis provides a detailed picture of the composition of grape seed PAs. | {
"pile_set_name": "PubMed Abstracts"
} |
Late extrusion of a temporomandibular joint prosthesis masquerading as a sebaceous cyst.
Complications of temporomandibular joint prostheses are not uncommon and have been well documented. We report a very late and rare complication following silastic TMJ replacement, believed to be the first implant extrusion through healthy skin. We are reminded that these implants can still cause problems to patients many years after initial surgery. | {
"pile_set_name": "PubMed Abstracts"
} |
A longer stay for the kissing disease: epidemiology of bacterial tonsillitis and infectious mononucleosis over a 20-year period.
Anecdotally, infectious mononucleosis is considered a more severe infection than bacterial tonsillitis, requiring a longer hospital stay. However, there is little in the literature comparing the epidemiology of the two conditions. This study aimed to compare the epidemiology of bacterial tonsillitis and infectious mononucleosis, in particular any differences in the length of in-patient stay. The hospital in-patient enquiry system was used to analyse patients admitted with bacterial tonsillitis and infectious mononucleosis between 1990 and 2009 inclusive. There was a total of 3435 cases over the 20 years: 3064 with bacterial tonsillitis and 371 with infectious mononucleosis. The mean length of stay was 3.22 days for bacterial tonsillitis and 4.37 days for infectious mononucleosis. The median length of stay for each condition was compared using the Mann-Whitney U non-parametric test, and a significant difference detected (p < 0.001). Patients with infectious mononucleosis have a significantly longer stay in hospital than those with bacterial tonsillitis. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of Percoll discontinuous density gradients vs SpermPrep II vs Sephadex G-50 gel infiltration on semen parameters.
There are various methods of separating sperm from seminal plasma for subsequent intrauterine insemination. Some of these same techniques have been employed for female sex selection. The purpose of the present study was to assess and compare semen parameters following Sephadex, Percoll, and SpermPrepII separation techniques. The SpermPrepII is also a Sephadex preparation but uses a different bead size, less Sephadex, and is a slightly quicker method. The specimens (n = 16) were initially evaluated for count, (C; x 10(6)/mL), percent motility (MO), grade of motility (GR; percent) and hypoosmotic swelling (HOS) test scores. Each specimen was then divided into three equal aliquots and prepared as follows: (1) layered onto a modified Percoll discontinuous density gradient column; (2) processed using SpermPrepII; and (3) filtered through a Sephadex gel filtration column (G-50). The results show all three treatments yielded lower (P < 0.01) counts as compared to control values, but no differences were noted among them. Percoll SpermPrepII increased MO (P < 0.01) whereas Sephadex reduced it (P = NS). Percoll and SpermPrepII improved the percentage of better quality sperm but not Sephadex; SpermPrepII was slightly higher than Percoll, but the difference was not significant. There were increases (P < 0.05) in HOS values in all experimental treatments, with SpermPrepII having the highest scores. However, this study did not show as many males with HOS scores below 50% as noted in other studies. Percoll and SpermPrepII seem to be equally effective methods for producing high-quality sperm for intrauterine insemination or in vitro fertilization. | {
"pile_set_name": "PubMed Abstracts"
} |
A comparison of the effects of alendronate and alfacalcidol on bone mineral density around the femoral implant and in the lumbar spine after total hip arthroplasty.
Several previous studies have demonstrated that bone mineral density loss around femoral implants is common, particularly in the proximal part of the femur, soon after total hip arthroplasty. The purpose of the present study was to compare the effects of alendronate and alfacalcidol on bone mineral density loss around the femoral implant and in the lumbar spine after total hip arthroplasty. The present study included sixty patients with osteoarthritis of the hip who had undergone a primary cementless total hip arthroplasty. We assigned these individuals to treatment with alendronate (n = 20), alfacalcidol (n = 18), or no medication (n = 22). Periprosthetic and lumbar spine bone mineral density was measured one week after surgery, and biochemical markers (bone-specific alkaline phosphatase and serum N-terminal telopeptides of type-1 collagen) were measured before surgery as a reference baseline. Subsequent measurements were performed at twelve, twenty-four, and forty-eight weeks after surgery. The periprosthetic measurement area in the femur was defined as Regions 1 to 7, which are consecutively located around the implant from the greater trochanter to the lesser trochanter and calcar. Bone mineral density in the alendronate group was maintained in all regions. In the alfacalcidol and no-medication groups, bone mineral density in Region 7 was lower than in Regions 3 to 6 throughout the study period (p < 0.0001 as a result of repeated measures analysis of variance). Bone mineral density in the lumbar spine in the alendronate and alfacalcidol groups was higher than in the no-medication group at forty-eight weeks. The serum level of N-terminal telopeptides of type-1 collagen in the alendronate group was lower than that in the no-medication group throughout the study period (p = 0.003, 0.02 and 0.005). Alendronate prevented bone mineral density loss around femoral implants, particularly in Region 7 (calcar), but alfacalcidol did not show any effects in any regions. However, bone mineral density losses in the lumbar spine were effectively prevented by either alendronate or alfacalcidol. | {
"pile_set_name": "PubMed Abstracts"
} |
Measurement of non-restorative sleep in insomnia: A review of the literature.
Non-restorative sleep (NRS) is a core symptom of insomnia, typically defined as a subjective feeling of being unrefreshed upon awakening. NRS symptoms have been less consistently studied than other symptoms of insomnia, and there is no consensus regarding measurement of NRS. Given its subjective nature, patient-reported outcome (PRO) instruments are important for evaluating NRS symptom severity and treatment-related changes. The objective of this literature review was to identify PROs used in insomnia populations that purport to measure NRS, and to evaluate their psychometric characteristics. A comprehensive review of PRO instruments used in insomnia was conducted; instruments were reviewed for NRS content by two independent reviewers; a total of 26 instruments identified to contain NRS content were retained for further review. Of the 26 instruments reviewed, 23 contained at least one item evaluating subjective nighttime aspects of NRS; 17 contained at least one item evaluating daytime aspects of NRS. Only the Sleep Assessment Questionnaire contained a specific NRS domain score. However, little published evidence was available regarding measurement properties of the NRS domain in insomnia populations. There is currently no reliable and well-validated PRO instrument available for specifically evaluating NRS symptom severity and response to interventions in insomnia populations. Reliable and valid measurement tools are needed to measure the symptom of NRS in insomnia. | {
"pile_set_name": "PubMed Abstracts"
} |
Gross alpha-particle activities in the ground waters in Western Anatolia.
The purpose of this study is to present data on gross alpha-particle activity, pH and conductivity in the ground waters in Western Anatolia. The gross alpha-particle activities in 27 ground water samples were determined by radiochemical carrier-precipitation methods. The gross alpha-particle activities of water samples were measured by using a ZnS (Ag) detector system. Measurable alpha-particle activity is present in all ground water samples, with one ground water sample having a gross alpha-particle activity > 0.55 Bq/L. | {
"pile_set_name": "PubMed Abstracts"
} |
[The investigation of efficacy and safety of Aerius to seasonal allergic rhinitis].
To evaluate the efficacy and safety of Aerius to seasonal allergic rhinitis. Using randomized, controlled method, treatment group 40 cases, oral administration Aerius 5 mg/d for 12 days; control group 35 cases, oral administration Aerius 5 mg/d for 12 days, investigating their efficacy and safety. The total effective rate of treatment group was 92.50%, while control group was 85.71%, there was significant difference between them (P<0.05). Significant improvements of seasonal allergic rhinitis nasal obstruction were seen in treatment group after using Aerius (P<0.05). The incidence of side effect of treatment group was 2.50%. Aerius is safe and can effectively reduction in nasal and nonnasal symptoms in patients with seasonal allergic rhinitis. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of TIPS on global end-diastolic volume and cardiac output and renal resistive index in ICU patients with advanced alcoholic cirrhosis.
The transjugular porto-systemic stent-shunt (TIPS) reduces portal pressure in cirrhotic patients and is used as a nonsurgical treatment for refractory ascites, recurrent variceal hemorrhage or hepatorenal syndrome. There are concerns regarding a negative impact on cirrhotic cardiomyopathy and deterioration of hyperkinetic circulatory dysfunction. We analyzed a prospectively maintained database containing hemodynamic data on cirrhotic ICU patients. Hemodynamic monitoring was performed using transpulmonary thermodilution (PiCCO, Pulsion Medical Systems, Munich, Germany). Renal perfusion was assessed by Doppler ultrasound during studies of portal and TIPS perfusion before and after the procedure. Complete data sets of 8 patients (4 male, 4 female, age 60 years (52-67), Child-Pugh-Turcotte score 10 (8-12)) were available. After TIPS, there was a substantial increase of GEDVI (646 ml/m2 (580-737) to 663 mL/m2 (643-792); p=0.036) that was even more pronounced at 24 hours (716 mL/m2 (663-821); P=0.012). CI increased from 3.3 L/min/m2 (3.1-4.2) to 3.9 L/min/m2 (3.6-5.3) (p=0.012) and 3.9 L/min/m2 (3.7-5.2) (p=0.017), respectively. There was a significant decrease of renal RI from 0.810 (0.781-0.864) to 0.746 (0.710-0.798) (p=0.028) and a transient increase of fractional excretion of sodium. SVRI (1737 dyn*s/cm5/m2 (1088 . 2115) vs. 1917 dyn*s/cm5/m2 (1368-2177) was not significantly altered immediately after TIPS but decreased to 1495 dyn*s/cm5/m2 (833- 1765) at 24 hours (p=0.036). There were no significant changes of mean arterial pressure (MAP). In conclusion, TIPS resulted in a pronounced increase of central blood volume. The observed hemodynamic effects are compatible with a preload driven increase of cardiac output and secondary decreases in SVRI and RI. | {
"pile_set_name": "PubMed Abstracts"
} |
The adrenal gland is a source of stress-induced circulating IL-18.
The present study compared plasma IL-18 levels between sham-operated and adrenalectomized mice following stress to investigate whether the adrenal gland contributes to the elevation of circulating IL-18 during stress. Two hours of stress provoked a robust, stressor-dependent, elevation of IL-18 mRNA and peptide in the adrenal gland in sham-operated mice. Consistently, levels of circulating mature IL-18 increased during stress and remained elevated for up to 6 h after stress. The stress-induced increase in circulating IL-18 was abolished by adrenalectomy. These findings demonstrate that the adrenal gland is required to achieve elevation of circulating IL-18 during stress. | {
"pile_set_name": "PubMed Abstracts"
} |
Pathogenesis of primary biliary cirrhosis.
Primary biliary cirrhosis is an enigmatic autoimmune disease that predominantly affects women. The serologic signatures of PBC are high titer antimitochondrial antibodies that are directed at the inner lipoyl domains of the 2-oxo-dehydrogenase enzymes, particularly PDC-E2. Of note, is that the antibody response and the CD4 and CD8 response, are all directed at a similar epitope, the inner lipoyl domain. This unique immunologic response suggests that modification of the inner lipoyl domain is associated with the immunogenetic basis of disease. | {
"pile_set_name": "PubMed Abstracts"
} |
Histologic and histobacteriologic observations of failed revascularization/revitalization therapy: a case report.
Mechanical debridement plays an important role in eliminating intracanal bacteria, such as biofilm on the canal walls and bacteria in the dentinal tubules. Mechanical debridement is not recommended for root canal disinfection in revascularization/revitalization therapy. Here we report a failed revascularization/revitalization case, which could be due to inadequate root canal disinfection without mechanical removal of biofilm and bacteria in dentinal tubules. A 6-year-old boy had a traumatic injury to tooth #9, which was avulsed and replanted within 40 minutes. The tooth subsequently developed a local swelling in the periapical area. The patient was referred to the Postgraduate Endodontic Clinic for revascularization/revitalization therapy on tooth #9. The treated tooth remained asymptomatic for 16 months and then developed pain and local periapical swelling. The oral surgeon extracted the revascularized/revitalized tooth. On request, the extracted tooth was processed for histologic and histobacteriologic examination. The tissue in the canal was completely destroyed. Most bacteria were observed in the apical portion and not in the coronal portion of the canal and formed biofilm on the canal walls and penetrated into the dentinal tubules. On the basis of histobacteriologic observations, the failure of revascularized/revitalized tooth could be due to inadequate root canal disinfection without mechanical debridement. It may be important to perform mechanical debridement as part of the revascularization/revitalization therapy to disrupt the biofilm on the canal walls and remove bacteria in the dentinal tubules because revascularization/revitalization therapy is able to increase thickening of the canal walls. | {
"pile_set_name": "PubMed Abstracts"
} |
Butalene and related compounds: aromatic or antiaromatic?
Density functional theory (DFT) has been used to study the first three members of the condensed cyclobutadienoid series, butalene (3), bicyclobutadienylene (12), and dicyclobutenobutalene (20). The first is planar and is judged "aromatic" by comparisons with suitable models using both energetic and magnetic criteria. The second is nonplanar, and not aromatic, but not so antiaromatic as cyclobutadiene (11). The third is slightly more antiaromatic and best viewed as a butalene fused to two cyclobutadiene rings; its properties are the sum of aromatic and antiaromatic components, like benzocyclobutadiene. Ring-opening transition states for both 3 and 12 have been located, and these are conrotatorily twisted. The ring-opening barrier for 12 is more than twice that for 3. Ring-opening of 20 involves ring inversion as the only barrier. | {
"pile_set_name": "PubMed Abstracts"
} |
The development of new concepts for assessing reproductive toxicity applicable to large scale toxicological programmes.
Large scale toxicological testing programmes which are currently ongoing such as the new European chemical legislation REACH require the development of new integrated testing strategies rather than applying traditional testing schemes to thousands of chemicals. The current practice of requiring in vivo testing for every possible adverse effect endanger the success of these programmes due (i) to limited testing facilities and sufficient capacity of scientific/technical knowledge for reproductive toxicity; (ii) an unacceptable number of laboratory animals involved (iii) an intolerable number of chemicals classified as false positive. A key aspect of the implementation of new testing strategies is the determination of prevalence of reproductive toxicity in the universe of industrial chemicals. Prevalences are relevant in order to be aware on the expected rate of false classification during the toxicological testing and to implement appropriate measures for their avoidance. Furthermore, a detailed understanding on the subendpoints affected by reproductive toxicants and the underlying mechanisms will lead to more science based testing strategies integrating alternative methods without compromising the protection of consumers. | {
"pile_set_name": "PubMed Abstracts"
} |
Folding of the cerebral cortex in mammals. A scaling model.
A model of cortical folding in mammals is presented. The model consists of a cube, superimposed on which are straight close-packed gyri, running the length of the cube. The cortex is represented by a thin layer of constant thickness. It proves possible, by adjusting the length, height, and width of the 'gyri' and the thickness of the 'cortex', to obtain a reasonable fit to the available empirical data (which extend over three to four orders of magnitude in brain weight). The model directs attention to possible features of the macroscopic organization of the mammalian brain which are novel and hitherto unremarked. | {
"pile_set_name": "PubMed Abstracts"
} |
Isolating vessels from the mouse brain for gene expression analysis using laser capture microdissection.
Studies of gene expression often examine a pool of RNA extracted from the diverse cell types making up a tissue. We have developed a method for isolating vessels from the brain in order to understand the changes occurring in the vessels during the pathogenesis of cerebral malaria. Vessels were visualised by incubating sections of mouse brain with a substrate for alkaline phosphatase. Vessels were collected by laser capture microdissection and the specificity was monitored by measuring the expression of cell-specific markers. RNA from the captured vessels was highly enriched in mRNA for genes associated with endothelial cells and pericytes. Measurement of indoleamine 2,3-dioxygenase mRNA indicated it was possible to detect changes in gene expression, due to malaria infection, occurring specifically within the vessels. Laser capture microdissection can be used to study changes in gene expression occurring at the blood-brain barrier. | {
"pile_set_name": "PubMed Abstracts"
} |
Mass spectrometry in the study of lysosomal storage disorders.
Lysosomal storage disorders represent a group of over 45 distinct genetic diseases, each one resulting from a deficiency of a particular lysosomal protein or, in a few cases, from non-lysosomal proteins that are involved in lysosomal biogenesis. A common biochemical feature of this group of disorders is the accumulation within lysosomes of undegraded or partially degraded substrates that are normally degraded within, and transported out of the lysosome. The particular substrates stored and the site(s) of storage vary with disease type and enzyme/protein deficiency. The nature of the substrate can be used to group the disorders into broad categories including the mucopolysaccharidoses, lipidoses, glycogenoses and oligosaccharidoses. These categories show many clinical similarities within groups as well as significant similarities between groups. For most lysosomal storage disorders the relationship between the stored substrates (type, amount and location) and the disease pathology is not well understood. The use of mass spectrometry and in particular tandem mass spectrometry provides a powerful tool for the investigation of stored substrates in this group of disorders. In this review we will describe the use of mass spectrometry for the analysis of stored substrates. We will discuss progress in the field, limitations of current methods, and summarise issues relating to the diagnosis and treatment of some of the more prevalent lysosomal storage disorders. | {
"pile_set_name": "PubMed Abstracts"
} |
Des-gamma-carboxyprothrombin: clinical effectiveness and biochemical importance.
Des-gamma-carboxyprothrombin (DCP) is an abnormal prothrombin with a decreased number of gamma-carboxylated glutamic acid residues in the Gla domain. DCP is also known to be an effective serological tumor marker for hepatocellular carcinoma (HCC), and highly sensitive methods of detecting serum DCP have enabled the detection of early and smallsized HCC in clinical settings. Several immunohistochemical studies have suggested that excessive production of DCP in HCC tissues may relate to worse tumor behavior such as the presence of vascular invasion and intrahepatic metastasis of HCC cells. Clinical availability of DCP, therefore, might be a more significant factor in the diagnosis of tumor behavior in HCC patients. Recently, some studies have suggested that DCP may play an important role in cancer progression via induction of cancer cell proliferation and angiogenesis around HCC tissues. Thus, DCP is expected to be effectively used not only as a tumor marker but also as a target of drug discovery. | {
"pile_set_name": "PubMed Abstracts"
} |
[The involvement of microbes in the etiology of respiratory diseases in infants 0 to 12 months old].
A study was worked out on the etiopathogenic relations and a more exact evaluation of the incidence of some agents: viruses, chlamydiae, mycoplasmas and rickettsiae, in the respiratory diseases of 0- to 12-month-old children. Serological analysis and immunofluorescence examination of pharyngeal cells were done. Results showed that parainfluenzae and respiratory syncytial viruses, chlamydiae, rickettsiae and mycoplasmas had an important part in the outbreak and maintaining of severe respiratory diseases in the 0- to 12-month-old children population. | {
"pile_set_name": "PubMed Abstracts"
} |
Adhesion measured on the attachment pads of Tettigonia viridissima (Orthoptera, insecta).
The tarsi of the cricket Tettigonia viridissima bear flexible attachment pads that are able to deform, replicating the profile of a surface to which they are apposed. This attachment system is supplemented by a secretion produced by epidermal cells and transported onto the surface of the pad through the pore canals of the pad cuticle. This study shows that the secretion alone is necessary, but not sufficient, for adhesion. To account for the full adhesive force, the deformation of the pad and the resulting changes in contact area were considered. In two series of experiments, the adhesive properties of the secretion and the adhesion of the whole pad were measured using a force tester, the sensitivity of which ranged from micronewtons to centinewtons. The adhesive forces of the secretion measured between a smooth sapphire ball with a diameter of 1.47 mm and a flat silicon surface ranged from 0.1 to 0.6 mN. In a control experiment on the silicon surface without secretion, no adhesive force was measured. There was no dependence of the adhesive force on the applied compressive force. When an intact pad was pulled off a flat silicon surface, the adhesive force increased with increasing applied compressive force, but it did not increase further once the applied force exceeded a certain value. The saturated adhesive force, ranging from 0.7 to 1.2 mN, was obtained at applied forces of 0.7-1.5 mN. The hemispherical surface of the pad had a larger contact area and demonstrated greater adhesion under a larger applied force. Adhesion became saturated when a pad was deformed such that contact area was maximal. The tenacity (the adhesive force per unit area) was 1.7-2.2 mN mm(-)(2). | {
"pile_set_name": "PubMed Abstracts"
} |
Effect of ambient temperature on serotonin, norepinephrine, and epinephrine contents in the pineal-paraphyseal complex of the soft-shelled turtle (Lissemys punctata punctata).
The aim of the present investigation was to ascertain the influence of ambient temperature on serotonin, norepinephrine, and epinephrine contents in the pineal-paraphyseal complex of soft-shelled turtles. Turtles were exposed to low (10 degrees) and high (32 degrees) temperatures for 7 days in an incubator with the same photoperiod (12L:12D). Control animals were also kept in the same incubator at 25 degrees for similar duration with the same photoperiod. The findings revealed that ambient temperatures significantly altered serotonin, norepinephrine, and epinephrine content in the pineal-paraphyseal complex of turtles. The results thus indicate that ambient temperature has some influence on pineal serotonin, norepinephrine, and epinephrine contents in soft-shelled turtles. | {
"pile_set_name": "PubMed Abstracts"
} |
[New antiepileptic drugs in childhood epilepsies: indications and limits].
Regarding the antiepileptic drugs (AEDs) in children, it was recently shown that they have a specific profile of efficacy and also of worsening according to the different epilepsy syndromes. However, the therapeutic profile of the most recent compounds is still not completely established in children because of the high number of syndromes and the difficulty to perform controlled studies in this age range. Controlled studies are most often first performed in adults and they begin in children whereas the new drug is already approved. However, some new AEDs dramatically improved seizure control, particularly in some severe epilepsy syndromes such as West syndrome and Lennox-Gastaut syndrome. Vigabatrin demonstrated a remarkable efficiency in infantile spasms whereas it tends to worsen myoclonic epilepsies, absence epilepsy and idiopathic partial epilepsy. Lamotrigine is efficient in absence epilepsy and symptomatic or cryptogenic generalized epilepsies such as Lennox-Gastaut syndrome and myoclonic astatic epilepsy. By contrast, lamotrigine increases the frequency of seizures in severe myoclonic epilepsy in infancy (Dravet syndrome) as well as in some cases of idiopathic partial epilepsy. Felbamate remains indicated as third line drug in refractory Lennox-Gastaut syndrome provided blood parameters are controlled. The therapeutic profile of oxcarbazepine is closed to that of carbamazepine. The efficacy of topiramate was demonstrated in partial epilepsy, but the other indications remain to be précised. Pediatric studies using gabapentin and tiagabine disclosed encouraging results in partial epilepsy. Clinical trials with stiripentol represent an example of strategy for developing a new AED in children; it recently demonstrated, in association with clobazaru, efficacy in a severe myoclonique epilepsy in infancy. | {
"pile_set_name": "PubMed Abstracts"
} |
A case of relapsed medulloblastoma treated with intensity-modulated radiotherapy and temozolomide.
We report a case of relapsed high-risk non-metastatic medulloblastoma in a 14-year-old boy, which was treated with intensity-modulated radiotherapy (IMRT) and temozolomide (TMZ). At the age of 11, the patient underwent an MRI-confirmed incomplete resection of a fourth-ventricle medulloblastoma, followed by conventional chemotherapy, craniospinal irradiation (55.8 Gy, 1.8 Gy/fraction) and then myeloablative chemotherapy followed by peripheral blood progenitor cell rescue. After 18 months of complete remission following the completion of chemotherapy, MRI showed a 2.5-cm mass in the olfactory notch. The patient underwent IMRT (45 Gy, 1.8 Gy/fraction) with concomitant administration of TMZ (180 mg/m2, 5 days every 21 days), which was well tolerated. After 5 cycles of TMZ, MRI showed complete remission with no evidence of the mass. TMZ was continued for another 5 cycles and then stopped. At 14 months from the completion of IMRT, a new MRI scan showed multiple nodular relapses around the fourth ventricle and the patient is currently treated with oral etoposide. | {
"pile_set_name": "PubMed Abstracts"
} |
Synthesis of helical and supplementary chirally doped PMO materials. Suitable catalysts for asymmetric synthesis.
Exciting helical mesoporous organosilicas including supplementary chirally doped moieties into their spiral walls were one-pot successfully synthesized with good structural order for, to the best of our knowledge, the first time. This one-step direct synthesis of helical chirally doped periodic mesoporous organosilica (PMO) materials was carried out by combination of a tartrate-based bis-organosilicon precursor with tetraethyl orthosilicate (TEOS) and two surfactants, cetyltrimethylammonium bromide and perfluoroctanoic acid (CTAB and PFOA). For comparison purposes, a conventional two-step postsynthetic grafting methodology was carried out. In this method, the chiral tartrate-based moieties were grafted onto the helical silica mesoporous materials previously prepared by the dual-templating approach (CTAB and PFOA). The chirally doped materials prepared by both methodologies exhibited helical structure and high BET surface area, pore size distributions, and total pore volume in the range of mesopores. Solid-state (13)C and (29)Si MAS NMR experiments confirmed the presence of the chiral organic precursor in the silica wall covalently bonded to silicon atoms. Nevertheless, one-pot direct synthesis led to a greater control of surface properties and presented larger incorporation of organic species compared with the two-step postsynthetic methodology. To further prove the potential feasibility of these materials in enantiomeric applications, Mannich diastereoselective asymmetric synthesis was chosen as catalytic test. In the case of the one-pot PMO material, the rigidity of the chiral ligand backbone provided by its integration into the inorganic helical wall in combination with the steric impediments supplied by the twisted geometry led to the reagents to adopt specific orientations. These geometrical constrictions resulted in an outstanding diastereomeric induction toward the preferred enantiomer. | {
"pile_set_name": "PubMed Abstracts"
} |
Glomerular mesangial cell adhesion to fibrinogen is mediated by alphavbeta3 integrin.
The biological behavior of glomerular mesangial cells is thought to play a critical role in human and experimental forms of mesangioproliferative glomerulonephritis. In these diseases, mesangial cells proliferate and produce increased amounts of extracellular matrix proteins, which can lead to glomerulosclerosis and end-stage renal disease. Mesangial cells interact with extracellular matrix proteins through integrin-mediated cell adhesion. Fibrinogen as a plasma-derived protein is known to be deposited in the mesangium of kidneys affected by mesangioproliferative glomerulonephritis. The adhesive interactions between fibrinogen and mesangial cells, however, have not been reported. Results in this work show that mesangial cells adhere to immobilized fibrinogen in an integrin-dependent fashion. This process was inhibited by the alphavbeta3-selective peptide cyclo-RGDFV and the monoclonal anti-beta3 integrin chain antibody F11. Ca(2+) ions are a known strong inhibitor of the fibrinogen-alphavbeta3 interaction, and mesangial cell adhesion did not occur when Ca(2+) was the only divalent cation present. Therefore, mesangial cell adhesion to fibrinogen is mediated by alphavbeta3 integrin, and divalent cations have a fundamental role in regulating this process. | {
"pile_set_name": "PubMed Abstracts"
} |
Delayed death after uncomplicated hot tub bathing in dogs and monkeys.
Prolonged heat exposure as in hot tub bathing, although frequently practiced, has occasionally resulted in fatalities that have been explained by an underlying disease. We explored the tolerance of hot water immersion of 60 min in five previously healthy animals (three dogs and two monkeys). With invasive monitoring, experimental body immersion in water at 40-45 degrees C, with core temperature kept at 40-42 degrees C for 60 min, caused no significant cardiovascular, pulmonary or metabolic changes during hyperthermia or for 2 h after return to normothermia. Then secondary deterioration occurred with progressive hypotension, petechial hemorrhages throughout the viscera, gross gastrointestinal hemorrhages and irreversible (hypovolemic) shock. These effects occurred earlier in the monkeys than in the dogs. This shock state did not respond to standard resuscitation attempts. One dog survived the secondary shock state. We conclude that during and after hot tub immersion, good initial tolerance to heat exposure can, several hours after return of normothermia, result in delayed secondary deterioration and death. We recommend that the mechanism of this delayed shock state with apparent capillary leakage be clarified. | {
"pile_set_name": "PubMed Abstracts"
} |
Maxillary Sinus Membrane Repair With Amnion-Chorion Barriers: A Retrospective Case Series.
Schneiderian membrane perforation is the most common complication of maxillary sinus augmentation procedures and has been associated with a variety of post-surgical problems. Multiple techniques to repair perforated Schneiderian membranes with materials such as connective tissue, buccal fat pads, and resorbable collagen membranes have been reported in the dental literature. Although these reparative options have proven successful, they are technique sensitive and time consuming. The aim of this case series is to present a simplified method of Schneiderian membrane perforation repair with amnion-chorion membranes and results obtained from nine cases using this technique. A consecutive retrospective record review was performed of all maxillary sinus augmentation cases performed during the past 5 years by the same board-certified private practice periodontist (DH). Seventy-seven cases were identified, with a total of 104 sinus augmentations, in which nine perforations were noted. None of the perforation cases were aborted midprocedure, and all perforations were repaired with amnion-chorion membranes. All cases were augmented with a combination of allograft and xenograft particulate bone. After an average healing time of 4.9 months, dental implants were placed in the grafted sinuses. This retrospective case series shows nine perforations during 104 lateral window maxillary sinus augmentation procedures. A total of 23 dental implants were placed in the augmented sinuses with perforated Schneiderian membranes, and one failure was noted according the Albrektsson success criteria. A total of 158 dental implants were placed in non-perforated augmented sinuses, with a total of three failures noted. | {
"pile_set_name": "PubMed Abstracts"
} |
Focal shifts in diffracted converging electromagnetic waves. I. Kirchhoff theory.
Starting with the vector formulation of the Kirchhoff diffraction theory, expressions for the total energy density distribution along the axis are presented without using any of the usual assumptions except the assumption made by Kirchhoff for the boundary conditions of a black screen. To make the Kirchhoff integral compatible with Maxwell's equations, a line integral around the edge of the aperture is added in the analysis. The consequence of ignoring the contribution of this line integral to the axial field distribution is examined numerically. The focal shift effect is investigated for both aplanatic systems and parabolic mirrors having an arbitrary numerical aperture (NA) and finite value of the Fresnel number. The combined effects of the Fresnel number and NA on the focal shift are evaluated, and the validity of the results is carefully checked by comparing the wavelength with the system dimensions. | {
"pile_set_name": "PubMed Abstracts"
} |
New insights into the clinical pharmacokinetics of trofosfamide.
This study focuses on the pharmacokinetics of trofosfamide (TRO) and metabolites after oral administration of TRO. Twelve patients with solid tumors and non-Hodgkin lymphomas were treated with 450 mg TRO orally for 7 days. TRO and the stable metabolites ifosfamide (IFO), cyclophosphamide (CYC), 2- and 3-dechloroethylifosfamide (2-DCE, 3-DCE) were determined by GC and the sum of the 4-OH-metabolites was measured by HPLC. A fast metabolism of TRO with a half-life of about 1 h was observed. IFO was the main stable metabolite, whereas CYC was only detected in minor quantities. The peak levels and the AUC of the 4-OH-metabolites were 9.5 and 4.3 times higher than observed after an equimolar IFO dose. Only 6% of the administered dose was recovered in urine within 24 hours as stable metabolites. TRO was under limit of detection. Our results confirm that dechloroethylation of TRO to IFO is a major metabolic pathway. Additionally, we found considerable 4-hydroxylation not shown previously. With respect to the low levels of IFO and CYC observed, the sum of 4-OH-metabolites cannot be explained by hydroxylation of these metabolites only. Hence, we assume a direct 4-hydroxylation of TRO occurring to a high extent. Bioavailability of TRO could not be calculated directly, because TRO is only available as an oral formulation. The bioavailability of oral IFO, however, is reported to be almost 100%. Therefore, after normalization of the dose, a bioavailability of 32% for IFO after oral TRO could be calculated. Thus, in contrast to previous reports, direct 4-hydroxylation of TRO seems to be the main metabolic pathway. | {
"pile_set_name": "PubMed Abstracts"
} |
New concepts in cyclosporine pharmacokinetic and dynamic monitoring: the impact of concomitant immunosuppression on target C2 concentrations.
There is a correlation between cyclosporine (CsA) pharmacokinetics (PK) and pharmacodynamics (PD), especially 2 hours after drug administration. To evaluate the relationship between CsA PK and PD profiles in two groups of stable renal transplant patients treated with CsA alone or CsA plus mycophenolate mofetil (CsA+MMF), so as to define the best target for C2 and clarify the impact of concomitant immunosuppression. Thirty-eight stable renal transplant recipients were treated with CsA (n=20) or CsA+MMF (n=18). Twelve nontreated normal healthy controls (NHC) were also included. Calcineurin activity (CNa), IL-2 production, and CsA levels were measured at 0 and 2 hours postdose. There were no significant differences in median CsA C2 values and CNa between the CsA alone and the CsA+MMF groups (388 microg/L and 497.5 microg/L and CNa 2h; 3.92% alkaline phosphatase [AP]; 3.94% AP, respectively). In vitro production of IL-2 was significantly lower in the CsA+MMF group than in the CsA group (median IL-2 2h: 280.52 ng/L, 169.48 ng/L, P<.001). The correlations (r) between C2 and CNa 2h were: CsA r=0.74; CsA+MMF r=0.84 (P<.001 in both cases). In stable renal transplant patients, median CsA C2 values below 500 microg/L were associated with inhibition of CNa and IL-2 synthesis. CNa and IL-2 production may be good biological markers of CsA immunosuppression. The measurement of CNa depends mainly on CsA concentration, whereas in vitro IL-2 production reflects the effect of both CsA and MMF. Further studies are necessary to define the optimal C2 target concentration and the possible impact of concomitant immunosuppression. | {
"pile_set_name": "PubMed Abstracts"
} |
Didactic value of the clinical evaluation exercise. Missed opportunities.
The clinical evaluation exercise (CEX), a direct observation of trainees' clinical skills, is a common method of house officer evaluation. During our studies of its reliability, the extent of the CEX's didactic value surfaced. This brief report describes the amount of information passed from the evaluator to the house officer in 73 CEXs. On average, evaluators made eight teaching points in postexamination sessions. However, there were as many points recorded on the CEX forms that evaluators never mentioned. In a subset of CEXs carried out with two observers present, agreement in the teaching points presented to the house officer within the pairs witnessing the same examination was 18%. Positive feedback constituted 9% to 12% of the points presented. Our observations suggest that the more systematic feedback mechanisms may enhance the didactic value of the CEX. | {
"pile_set_name": "PubMed Abstracts"
} |
Acute myocardial infarction as a complication of cardiac sarcoma: a rare presentation.
We present a case of left atrial sarcoma presenting as acute myocardial infarction. Echocardiographic finding of left atrial mass, absence of angiographic evidence of coronary atherosclerotic disease, and incidental finding of prior splenic infarct strongly point to coronary emboli. The relatively low incidence of cardiac sarcoma and the uncommon presentation of cardiac sarcoma as myocardial infarction make this case unique. | {
"pile_set_name": "PubMed Abstracts"
} |
Grayscale and pulsed Doppler characteristics of non-cirrhotic portal fibrosis: a preliminary report.
Non-cirrhotic portal fibrosis is a common cause of portal hypertension in India. Inspite of this there is no published data delineating the imaging findings in patients with this disease. The aim of this study was to evaluate Grayscale and pulsed Doppler findings of the portal venous system in non-cirrhotic portal fibrosis. Grayscale and Doppler sonography was performed on 19 patients with clinically and histologically proven non-cirrhotic portal fibrosis. Portal venous flow was determined along with the portal venous diameter, congestive index of the portal vein, and the splenic index. Twenty normal control subjects were also studied. Doppler analysis revealed features of portal hypertension. These included increased portal venous flow, and congestive index (1062.1 +/- 199.7 vs 724.4 +/- 181.2; and 0.140 +/- 0.070 vs 0.049 +/- 0.011 respectively) when compared with the control group. Increased splenic index and dilatation of the portal venous diameter was also present (116.4 +/- 29.8 vs 45.8 +/- 7.0; and 14.3 +/- 1.5 vs 10.3 +/- 1.1 respectively). Grayscale findings demonstrated echogenic and thickened intrahepatic portal venous branches in 10 cases. Subcapsular atrophy of the liver was seen in three cases. Varices were identified in 15 cases. Patients with non-cirrhotic portal fibrosis have features of portal hypertension at the time of presentation. Ultrasonic findings that include echogenic, thickened portal venous tracts and subcapsular liver atrophy were frequently observed in such cases and were not seen in the control group. | {
"pile_set_name": "PubMed Abstracts"
} |
Biopsy results of new calcifications in the postirradiated breast.
A breast biopsy was performed in 19 patients for the finding of new mammographic calcifications without an associated palpable or mammographic mass after breast-conserving surgery and definitive irradiation for early stage breast cancer. The interval postradiotherapy was 9 to 96 months with a median of 34 months. Eleven of the biopsy specimens (58%) were positive for recurrent breast cancer and eight (42%) were negative. The pathologic results from the positive biopsy specimens showed four with invasive ductal carcinoma, two with microinvasive ductal carcinoma, four with intraductal carcinoma, and one with lobular carcinoma in situ (LCIS). Treatment consisted of mastectomy in eight patients, mastectomy plus chemotherapy in one patient, and biopsy for the patient with LCIS. One patient refused a recommended mastectomy. All 11 patients with recurrent carcinoma are alive with no evidence of disease after salvage therapy, although follow-up is short (median, 14 months; range, 0-48 months). Calcifications which developed in a quadrant different from the initial tumor tended to be malignant with four of five having a positive biopsy result. Microcalcifications were not commonly associated with the initial tumor with only five of 19 having microcalcifications. These results show that the development of new calcifications in the postirradiated breast is associated with a positive biopsy rate of 58% and that the tumors which are found tend to be early and potentially salvageable. The positive biopsy rate of 58% in the postirradiated breast is in marked contrast to the lower positive biopsy rate for microcalcifications in the nonirradiated breast as reported in the literature. | {
"pile_set_name": "PubMed Abstracts"
} |
[Value of Doppler velocimetry of the umbilical artery, aorta, cerebral artery, and uterine artery in pathological pregnancies].
This prospective study try to evaluate the predictive value of Doppler umbilical artery in the diagnosis of intra-uterine retardation (IUGR) and fetal distress requiring operative delivery. We use the blood flow class (BFC) to characterize the blood velocity waveform. Like others, we found a strong negative correlation between the blood flow class and fetal weight at delivery. When the umbilical BFC is abnormal in case of suspicion of IUGR, the diagnosis is confirmed at delivery in 100%, but in case of umbilical BFC 0, we found only 53% of IUGR. If we consider the mode of delivery, we found 7% of cesarean section for fetal distress in BFC 0, 38% in BFC1 and 78% in BFC 2-3. We had 5 fetal death, two cases with malformations, non-immunologic hydrops and trisomy 18. The others three were severe IUGR, in all cases the umbilical Doppler BFC was abnormal. Another parameter was significantly modified in case of fetal distress: the cerebro-placental index. This confirm the brain sparing phenomenon. We found a prevalence of IUGR of 43% in the high risk pregnancies group. The positive predictive value of umbilical Doppler is 82%, but the sensitivity is low, 44%. The efficacy of umbilical Doppler to predict the occurrence of operative delivery for fetal distress is better, because the prevalence is lower, 18%, the positive predictive value is 60% and the sensibility 70%. There is non doubt that, fetal blood flow is a secondary test of great value in identifying fetuses at risk of perinatal hypoxia. | {
"pile_set_name": "PubMed Abstracts"
} |
[Metabolic adaptations to prolonged fasting in birds].
Because they tolerate remarkably long periods of fasting, birds appear as interesting models to study the mechanisms for decreasing metabolic rate and sparing proteins. In particular, the comprehension of the metabolic changes occurring during the three periods of fasting which have been characterized, might be helpful for determining the possible role of ketone bodies in the adaptation to long-term fasting. | {
"pile_set_name": "PubMed Abstracts"
} |
Outcome and prognostic factors following adjuvant prednisone/vinblastine chemotherapy for high-risk canine mast cell tumour: 61 cases.
The medical records of 61 dogs with MCT at high risk for metastasis that were treated with prednisone and VBL following excision+/-radiation therapy were reviewed, and median disease-free interval (DFI), median overall survival time (OS) and prognostic factors assessed. Adverse effects, mostly mild, were noted in 26% of patients, usually after the first VBL dose. 6.5% experienced severe neutropenia. The DFI was 1305 days, and the OS was not reached, with 65% alive at 3 years. 100% of dogs with "high-risk" grade II MCT were alive at 3 years. The OS for dogs with grade III MCT was 1374 days. Histologic grade, location (mucous membrane vs. skin) and use of prophylactic nodal irradiation predicted outcome. Prednisone and VBL chemotherapy is well tolerated, and results in good outcomes following surgery in dogs with MCT at high risk for metastasis. High-grade and mucocutaneous tumors had a worse outcome, and the use of prophylactic nodal irradiation appeared to improve outcome in this group of dogs. | {
"pile_set_name": "PubMed Abstracts"
} |
Ischemic preconditioning increases myocardial O-GlcNAc glycosylation.
Through the hexosamine biosynthetic pathway (HBP) proteins are modified by O-linked-β-N-acetylglucosamine (O-GlcNAc), which acts as a stress sensor. Augmentation of O-GlcNAc confers cardioprotection against ischemia- reperfusion injury, but its role in ischemic preconditioning (IPC) is unknown. Azaserine and alloxan are unspecific blockers of the HBP and have been used to block the cardioprotective effects of O-GlcNAc. We hypothesized that IPC reduces infarct size and increases O-GlcNAc levels in hearts subjected to ischemia-reperfusion injury, and that these effects could be blocked by azaserine and alloxan. Isolated rat hearts subjected to 40 min global ischemia and 120 min reperfusion were randomized to control, IPC, IPC + azaserine or alloxan, or control + azaserine or alloxan. The effects on infarct size, hemodynamic recovery, myocardial O-GlcNAc levels, and HBP enzyme activities were determined. IPC reduced infarct size, increased O-GlcNAc levels, O-GlcNAc-transferase levels, and O-GlcNAc-transferase activity. Azaserine and alloxan did not block the effect of IPC on O-GlcNAc levels and O-GlcNAc-transferase activity. IPC increased O-GlcNAc levels though increased O-GlcNAc-transferase expression and activity. Azaserine and alloxan failed to block these effects presumably due to poor specificity and sensitivity of the blockers, and IPC-mediated cardioprotection may therefore still be dependent on O-GlcNAc. | {
"pile_set_name": "PubMed Abstracts"
} |
Search for correlations between FBN1 genotype and complete Ghent phenotype in 44 unrelated Norwegian patients with Marfan syndrome.
In monogenic disorders, correlation between genotype and phenotype is a premise for predicting prognosis in affected patients. Predictive genetic testing may enable prophylaxis and promote clinical follow-up. Although Marfan syndrome (MFS) is known as a monogenic disorder, according to the present diagnostic criteria a mutation in the gene FBN1 is not sufficient for the diagnosis, which also depends on the presence of a number of clinical, radiological, and other findings. The fact that MFS patient cohorts only infrequently have been examined for all relevant phenotypic manifestations may have contributed to inconsistent reports of genotype-phenotype correlations. In the Norwegian Study of Marfan syndrome, all participants were examined for all findings contained in the Ghent nosology by the same investigators. Mutation identification was carried out by robot-assisted direct sequencing of the entire FBN1 coding sequence and MLPA analysis. A total of 46 mutations were identified in 44 unrelated patients, all fulfilling Ghent criteria. Although no statistically significant correlation could be obtained, the data indicate associations between missense or splice site mutations and ocular manifestations. While mutations in TGF-domains were associated with the fulfillment of few major criteria, severe affection was indicated in two cases with C-terminal mutations. Intrafamilial phenotypic variation among carriers of the same mutation, suggesting the influence of epigenetic facors, complicates genetic counseling. The usefulness of predictive genetic testing in FBN1 mutations requires further investigation. | {
"pile_set_name": "PubMed Abstracts"
} |
Nucleocytoplasmic shuttling of STAT transcription factors.
The signal transducer and activator of transcription (STAT) proteins have initially been described as cytoplasmic proteins that enter the nucleus only after cytokine treatment of cells. Contrary to this assumption, it was demonstrated that STATs are constantly shuttling between nucleus and cytoplasm irrespective of cytokine stimulation. This happens both via carrier-dependent as well as carrier-independent transportation. Moreover, it was also recognized that cytokine stimulation triggers nuclear retention of dimeric STATs, rather than affecting the rate of nuclear import. In summary, it is increasingly being appreciated that STAT nucleocytoplasmic cycling determines the quality of cytokine signaling and also constitutes an important area for microbial intervention. | {
"pile_set_name": "PubMed Abstracts"
} |
Alterations in regional brain volume and individual MRI-guided perfusion in normal control, stable mild cognitive impairment, and MCI-AD converter.
Regional differences in tissue volume and perfusion in brains of individuals with mild cognitive impairment (MCI) versus normal healthy age-matched controls (NC), and the differences between MCI-AD converters and stable MCI patients were investigated. MRI and SPECT scans were performed on 13 MCI (74+6 years) and 12 NC (75+4 years). Of the MCI patients, 10 were followed for up to three years and 4 subsequently converted to Alzheimer's disease (AD). Episodic memory function was assessed using tests of delayed recall for word lists and stories. The volume reductions and hypoperfusion were mainly confined to the medial temporal lobe (MTL) of MCI patients and associated with worse scores on memory tests. Perfusion in the corpus callosum and the gray matter of frontal, lateral temporal, parietal or occipital lobe was not significantly affected in MCI. The 4 MCI-AD converters had relatively low MTL structural volume and perfusion compared to their stable peers. | {
"pile_set_name": "PubMed Abstracts"
} |
Cortical grey matter sodium accumulation is associated with disability and secondary progressive disease course in relapse-onset multiple sclerosis.
Sodium (23Na)-MRI is an emerging imaging technique to investigate in vivo changes in tissue viability, reflecting neuroaxonal integrity and metabolism. Using an optimised 23Na-MRI protocol with smaller voxel sizes and improved tissue contrast, we wanted to investigate whether brain total sodium concentration (TSC) is a biomarker for long-term disease outcomes in a cohort of patients with relapse-onset multiple sclerosis (MS), followed from disease onset. We performed a cross-sectional study in 96 patients followed up ~ 15 years after a clinically isolated syndrome (CIS) and 34 healthy controls. Disease course was classified as CIS, relapsing-remitting MS or secondary progressive MS (SPMS). We acquired 1H-MRI and 23Na-MRI and calculated the TSC in cortical grey matter (CGM), deep grey matter, normal-appearing white matter (WM) and WM lesions. Multivariable linear regression was used to identify independent associations of tissue-specific TSC with physical disability and cognition, with adjustment for tissue volumes. TSC in all tissues was higher in patients with MS compared with healthy controls and patients who remained CIS, with differences driven by patients with SPMS. Higher CGM TSC was independently associated with Expanded Disability Status Scale (R2=0.26), timed 25-foot walk test (R2=0.23), 9-hole peg test (R2=0.23), Paced Auditory Serial Addition Test (R2=0.29), Symbol Digit Modalities Test (R2=0.31) and executive function (R2=0.36) test scores, independent of grey matter atrophy. Sodium accumulation in CGM reflects underlying neuroaxonal metabolic abnormalities relevant to disease course heterogeneity and disability in relapse-onset MS. TSC and should be considered as an outcome measure in future neuroprotection trials. | {
"pile_set_name": "PubMed Abstracts"
} |
[Short introduction to gene imprinting].
Genomic imprinting refers to the genetic non-equivalence of mammalian paternal and maternal genomes. It leads to differential marking of gene alleles in parental gamets. This marking leads to differential expression of imprinted genes during embryonal development and in adult life. Usually one allele of an imprinted gene is active while the other is silent. Genomic imprinting renders mammals haploid for imprinted genes and thereby causes certain genetic diseases. These can be due to a variety of genetic events, such as loss or mutation of the active imprinted gene allele, disruption of imprinting in parental gamets so that both alleles are imprinted in the same way, or inheritance of a chromosome or part of chromosome bearing imprinted genes from a single parent. Several severe human genetic disorders are associated with imprinted genes, including Beckwith-Wiedemann, Prader-Willi and Angelman syndromes. In this review a glimpse of the complexity of genetic mechanisms, which lead to the differential transcription of imprinted genes, is presented. | {
"pile_set_name": "PubMed Abstracts"
} |
Generation and expression in plants of a single-chain variable fragment antibody against the immunodominant membrane protein of Candidatus phytoplasma aurantifolia.
Witches' broom of lime is a disease caused by Candidatus Phytoplasma aurantifolia, which represents the most significant global threat to the production of lime trees (Citrus aurantifolia). Conventional disease management strategies have shown little success, and new approaches based on genetic engineering need to be considered. The expression of recombinant antibodies and fragments thereof in plant cells is a powerful approach that can be used to suppress plant pathogens. We have developed a single-chain variable fragment antibody (scFvIMP6) against the immunodominant membrane protein (IMP) of witches' broom phytoplasma and expressed it in different plant cell compartments. We isolated scFvIMP6 from a naïve scFv phage display library and expressed it in bacteria to demonstrate its binding activity against both recombinant IMP and intact phytoplasma cells. The expression of scFvIMP6 in plants was evaluated by transferring the scFvIMP6 cDNA to plant expression vectors featuring constitutive or phloem specific promoters in cassettes with or without secretion signals, therefore causing the protein to accumulate either in the cytosol or apoplast. All constructs were transiently expressed in Nicotiana benthamiana by agroinfiltration, and antibodies of the anticipated size were detected by immunoblotting. Plant-derived scFvIMP6 was purified by affinity chromatography, and specific binding to recombinant IMP was demonstrated by enzyme-linked immunosorbent assay. Our results indicate that scFvIMP6 binds with high activity and can be used for the detection of Ca. Phytoplasma aurantifolia and is also a suitable candidate for stable expression in lime trees to suppress witches' broom of lime. | {
"pile_set_name": "PubMed Abstracts"
} |
Needle/wire lung nodule localization for thoracoscopic resection.
Small lung nodules undiagnosed by percutaneous needle biopsy have traditionally gone to thoracotomy for diagnosis. We describe a technique using computed tomographic needle/wire lung localization of these nodules, to be resected using video-assisted thoracoscopy. This is less invasive and less painful than thoracotomy and provides for cost-effective definitive diagnosis. | {
"pile_set_name": "PubMed Abstracts"
} |
Characteristics of habituation of the sympathetic skin response to repeated electrical stimuli in man.
To study the effect of repeating electrical peripheral nerve stimulation on latency, duration and amplitude of the sympathetic skin response (SSR). SSRs were elicited in all limbs by median and peroneal nerves stimuli. In 10 subjects, 20 stimuli were applied at random time intervals (15-20 s). Another test was performed in 7 subjects using the same protocol, but switching the stimulation site every 5 or 10 stimuli without warning. The mean amplitude of right palmar response to right peroneal nerve stimulation decreased from 5.05+/-0.76 (SEM) mV at the first stimulus to 1.23+/-0.42 mV at the 20th stimulus (P<0.001). The latency did not change significantly (1473+/-82 to 1550+/-90 ms, P>0.1), while the duration increased (1872+/-356 to 3170+/-681 ms, P<0.001). Stimulation and recording at other sites showed similar trends. Changing the stimulation site failed to alter the adaptation process in terms of amplitude, latency or duration. Changes in amplitude and duration of the SSRs to repeated electrical stimuli can occur in presence of constant latency and appear to be independent of the source of sensory input. Peripheral sweat gland mechanisms may be involved in the loss of amplitude and increase in duration of the SSR during habituation. | {
"pile_set_name": "PubMed Abstracts"
} |
Activation of dormant cancer cells in the prostates and seminal vesicles of Lobund-Wistar rats.
Lobund-Wistar (L-W) rats are unique in their susceptibility to spontaneous and induced metastasizing adenocarcinomas in the prostate-seminal vesicle (P-SV) complex. Tumors were induced in 70-100% of rats by a combination of i.v. inoculated methylnitrosourea (MNU) followed by a series of subcutaneous slow-release implants of testosterone propionate (TP). Adenocarcinoma cells initiated by MNU in 3-month-old L-W rats were activated significantly by implants of the promoter, TP, after intervening periods of 3, 6 and 12 months following their exposures to MNU. The longer the time between MNU and TP, the shorter the subsequent latent period. Control rats inoculated with MNU (without TP) did not develop tumors during the observation period of 12 months, and their dormant tumor cells were activated by a single implant of TP, thereby eliciting P-SV tumors. | {
"pile_set_name": "PubMed Abstracts"
} |
Anaplastic large cell lymphoma of the esophagus in a pediatric patient.
We report a 3-year-old boy who initially presented with abdominal pain and was subsequently found to have an esophageal perforation. The child did not respond to conservative management, and subsequent lymphadenopathy led to a lymph node biopsy demonstrating an anaplastic lymphoma kinase (ALK)+ anaplastic large cell lymphoma. Esophageal perforation and thickening is most commonly seen in children with a history of esophageal intervention or foreign body/caustic ingestion. Esophageal involvement in children with non-Hodgkin lymphoma (NHL) has not, to our knowledge, been reported in the literature. This case illustrates an unusual presentation of pediatric NHL. | {
"pile_set_name": "PubMed Abstracts"
} |
Strychnine-sensitive glycine receptors depress hyperexcitability in rat dentate gyrus.
Previously we have shown that strychnine-sensitive glycine-gated chloride channels (GlyRs) are functionally expressed by CA1 pyramidal cells and GABAergic interneurons in mature rat hippocampal slices. We now report that glycine application to dentate granule cells and hilar interneurons recorded in acute slices from adolescent rats elicits a strychnine-sensitive current similar to glycine-mediated currents recorded in area CA1, indicating that GlyRs are also present on neurons in the dentate gyrus. This finding suggests that GlyRs have a widespread distribution in the hippocampal region. The physiological role of GlyRs in forebrain is unclear, but it is possible that these receptors mediate neuronal inhibition, similar to gamma-aminobutyric acid-A (GABA(A)) receptors and thus could be a novel target for antiepileptic therapy. Therefore we tested the hypothesis that activation of inhibitory GlyRs could suppress neuronal hyperexcitability in dentate, a brain region vulnerable to epileptic activity. In whole-cell current-clamp recordings of granule cells, we observed a membrane potential hyperpolarization followed by cessation of the action potential firing pattern in hyperexcitable slices induced by elevated extracellular K(+) or by blocking GABA(A) receptors with bicuculline. The GlyR antagonist, strychnine, prevented the antiepileptic effect of glycine. These results demonstrate that glycine, acting at GlyRs, elicits neuronal inhibition in dentate. Further, our findings suggest the possibility that these receptors could be a therapeutic target for the treatment of epilepsy. | {
"pile_set_name": "PubMed Abstracts"
} |
Seasonal fluctuations in plasma testosterone levels and diurnal song activity in free-living male great tits.
The annual course of plasma testosterone (T) and song activity during the day was examined in free-living male great tits (Parus major) in a single year and in a single population. We provide the first study in this species investigating plasma T during the breeding season among and within breeding stages. As expected for a temperate-zone monogamous species, plasma T levels showed a pronounced peak in March at the start of the breeding season and fast decreasing levels thereafter to moderate levels in the parental phase. Peak plasma T levels coincided with the period of intense territoriality but were not sustained during the egg laying period, suggesting that the annual T peak is more related to territoriality than to mate fertility or dawn song. After basal plasma T levels during summer and autumn, T levels started to increase from December onwards, possibly related to the reappearance of territoriality and pair formation in early winter. Within the feeding stage, males breeding later in the season had lower T levels than males breeding earlier, independent of nestling age, male condition, or the percentage of fertile females in the population. Also, during the nestling stage, T levels increased as nestlings were older, while controlling for time in season, male body condition, and availability of fertile females. The annual T pattern was highly correlated with the annual pattern of day-time song activity in the population. However, a small resurgence in autumn of day-time song activity in the population was not accompanied by a noticeable increase in plasma T in the captured males. Peak plasma T levels did not occur during the egg laying stage when dawn song activity is maximal, suggesting that there is no strong causal relationship between the two. | {
"pile_set_name": "PubMed Abstracts"
} |
The provision of information for patients prior to cataract surgery.
This clinical audit evaluates the provision of patient information. It also establishes patients' views on satisfaction regarding the information provided at nurse-led preoperative assessment prior to giving informed consent for cataract surgery. A quantitative descriptive audit was conducted using a questionnaire, by which the questions reflected the baseline standards. A convenience sample of 75 postcataract patients took part in this retrospective audit. All 75 patients stated that they were either 'very satisfied' or 'satisfied' with both the verbal explanation and written information received, and identified the preoperative assessment nurse as the main source of information. | {
"pile_set_name": "PubMed Abstracts"
} |
Effect of moderate exercise on immune senescence in men.
The purpose of this study was to examine the relationship between active compared to inactive lifestyles and immunocompetence in men. Subjects, all male volunteers, regularly exercising moderately were separated into three age groups: young (20-39 years), middle-aged (40-59 years) and elderly (more than 60 years). Age-matched sedentary male subjects served as controls in each group. Immunological assessments were, total leucocyte count, lymphocyte subpopulation counts, natural killer cell activity and neutrophilic phagocytosis. Total leucocyte and T-cell (CD3+) counts were not significantly different among the groups. Among T-cell subsets, there was a slight increase in helper T-cell (CD3+CD4+) and a decrease in cytotoxic/suppressor T-cell (CD3+CD8+) concentrations in the older sedentary subjects, resulting in an age-associated significant increase in the CD4:CD8 ratio among those control groups. However, among the exerciser groups, no such increase and decrease in the T-cell subpopulations or an age-related increase of the CD4:CD8 ratio were observed. Considering the components of innate immunity, the concentration of NK-cells (CD16+CD56+) significantly increased in the elderly exercisers, compared to that of the age-matched control subjects, or of the young group. The phagocytotic activity of neutrophils showed an age-associated decline, but of lesser degree in the elderly exercisers than in the elderly controls. Taken together, these results suggest that habitual and moderate training in later life is associated with a lesser age-related decline in certain aspects of circulating T-cell function and innate immunity. | {
"pile_set_name": "PubMed Abstracts"
} |
Purification of recombinant human COX-1 and COX-2.
Human recombinant COX-1 and COX-2 was prepared in a purified form. The genes were cloned and expressed in insect cells, extracted by detergents, and purified by ion-exchange followed by size exclusion chromatography. Insect cells from 10 L fermentation yielded 2.3 mg of COX-1 with an overall yield of 75%, and 29 mg of COX-2 with an overall yield of 45%. Enzyme prepared in this manner was fully active and proved to be useful in biophysical studies including direct binding studies. The COX-2 provided material that was subsequently used in X-ray crystallography studies. | {
"pile_set_name": "PubMed Abstracts"
} |
Quantitative EEG in babies at risk for hypoxic ischemic encephalopathy after perinatal asphyxia.
To evaluate an electroencephalography (EEG)-based index, the Cerebral Health Index in babies (CHI/b), for identification of neonates with high Sarnat scores and abnormal EEG as markers of hypoxic ischemic encephalopathy (HIE) after perinatal asphyxia. This is a retrospective study using 30 min of EEG data collected from 20 term neonates with HIE and 20 neurologically normal neonates. The HIE diagnosis was made on clinical grounds based on history and examination findings. The maximum-modified clinical Sarnat score was used to grade HIE severity within 72 h of life. All neonates underwent 2-channel bedside EEG monitoring. A trained electroencephalographer blinded to clinical data visually classified each EEG as normal, mild or severely abnormal. The CHI/b was trained using data from Channel 1 and tested on Channel 2. The CHI/b distinguished among HIE and controls (P<0.02) and among the three visually interpreted EEG categories (P<0.0002). It showed a sensitivity of 82.4% and specificity of 100% in detecting high grades of neonatal encephalopathy (Sarnat 2 and 3), with an area under the receiver operator characteristic (ROC) curve of 0.912. CHI/b also identified differences between normal vs mildly abnormal (P<0.005), mild vs severely abnormal (P<0.01) and normal vs severe (P<0.002) EEG groups. An ROC curve analysis showed that the optimal ability of CHI/b to discriminate poor outcome was 89.7% (sensitivity: 87.5%; specificity: 82.4%). The CHI/b identified neonates with high Sarnat scores and abnormal EEG. These results support its potential as an objective indicator of neurological injury in infants with HIE. | {
"pile_set_name": "PubMed Abstracts"
} |
Impact of nasal conditions on chronic otitis media: a cross-sectional study in Koreans.
We evaluated the relationship between pathological nasal conditions and pathological middle ear conditions on a large-scale epidemiological basis, conducted as a cross-sectional study at the population level using Korean National Health and Nutrition Examination Survey (KNHANES) data. A cross-sectional study. In a retrospective cohort study, we analyzed data from the KNHANES (2008-2012), which used a stratified, multi-stage, probability-cluster sampling method in a rolling sampling survey of South Korean citizens. The prevalences of nasal and middle ear pathologies were evaluated. Chronic postnasal drip, chronic hypertrophic rhinitis, and nasal polyps were correlated with the presence of middle ear pathologies; allergic rhinitis was negatively correlated. The prevalences of chronic otitis media and septal deviation showed no relationship. Only nasal polyps showed a tendency to be associated with cholesteatomatous otitis media in patients with COM. Our results indicate the need for a nasal assessment when evaluating COM. Additionally, correction of nasal pathologies may be useful with the surgical treatment of COM to improve patient success rates and satisfaction. | {
"pile_set_name": "PubMed Abstracts"
} |
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