Datasets:

---

siddharthtumre

/

Revised-JNLPBA

like 0

[ "Both", "LAM", "-and", "LPS-inducible", "IL-6", "promoter", "activity", "was", "localized", "to", "a", "DNA", "fragment", ",", "positions", "-158", "to", "-49", ",", "by", "deletion", "analysis", "and", "chloramphenicol", "acetyltransferase", "assay", "." ]

[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]

[ "Two", "nuclear", "factor", "NF-IL6", "(", "positions", "-153", "to", "-145", "and", "-83", "to", "-75", ")", "and", "one", "nuclear", "factor", "NF-kappa", "B", "(", "positions", "-72", "to", "-63", ")", "motifs", "are", "present", "within", "this", "fragment", "." ]

[ 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Site-directed", "mutagenesis", "of", "one", "or", "more", "of", "these", "motifs", "within", "the", "IL-6", "promoter", "demonstrated", "that", "each", "has", "positive", "regulatory", "activity", "and", "that", "they", "could", "act", "in", "a", "function-", "and", "orientation-independent", "manner", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Deletion", "of", "all", "three", "elements", "abolished", "inducibility", "of", "IL-6", "promoter", "activity", "by", "both", "LAM", "and", "LPS", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "conclude", "that", "the", "NF-IL6", "and", "NF-kappa", "B", "sites", "mediate", "IL-6", "induction", "in", "response", "to", "both", "LPS", "and", "LAM", ",", "acting", "as", "bacterial", "or", "mycobacterial", "response", "elements", "." ]

[ 0, 0, 0, 0, 1, 0, 1, 2, 2, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Regulation", "of", "CD14", "expression", "during", "monocytic", "differentiation", "induced", "with", "1", "alpha", ",", "25-dihydroxyvitamin", "D3", "." ]

[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "CD14", ",", "a", "monocyte/macrophage", "receptor", "for", "the", "complex", "of", "LPS", "and", "LPS", "binding", "protein", ",", "is", "a", "differentiation", "marker", "for", "the", "monocyte/macrophage", "lineage", "." ]

[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "We", "have", "analyzed", "the", "regulation", "of", "CD14", "expression", "during", "1", "alpha", ",", "25-dihydroxyvitamin", "D3", "(", "VitD3", ")", "-induced", "monocytic", "differentiation", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Using", "FACS", ",", "Northern", "blotting", ",", "and", "nuclear", "run-on", "analyses", ",", "we", "demonstrate", "that", "the", "up-regulation", "of", "CD14", "expression", "during", "monocytic", "cell", "maturation", "is", "regulated", "mainly", "at", "the", "level", "of", "gene", "transcription", ",", "and", "that", "new", "protein", "synthesis", "is", "required", "for", "CD14", "induction", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "We", "have", "recently", "cloned", "the", "CD14", "5", "'", "upstream", "sequence", "and", "demonstrated", "its", "tissue-specific", "promoter", "activity", "." ]

[ 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0 ]

[ "Using", "stable", "transfection", "of", "the", "monocytoid", "U937", "cell", "line", "with", "a", "series", "of", "deletion", "mutants", "of", "the", "CD14", "5", "'", "upstream", "sequence", "coupled", "to", "a", "reporter", "gene", "construct", ",", "we", "show", "that", "bp", "-128", "to", "-70", "is", "the", "critical", "region", "for", "the", "induction", "of", "CD14", "expression", "." ]

[ 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "This", "region", "contains", "two", "binding", "sites", "for", "the", "Sp1", "transcription", "factor", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "A", "3-bp", "mutation", "at", "the", "distal", "Sp1-binding", "site", "not", "only", "eliminates", "Sp1", "interaction", ",", "but", "also", "abolishes", "most", "of", "the", "VitD3", "induction", "of", "CD14", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "Electrophoretic", "mobility", "shift", "analysis", "does", "not", "detect", "a", "direct", "interaction", "of", "the", "CD14", "distal", "Sp1-binding", "site", "with", "the", "vitamin", "D3", "receptor", "and", "its", "partner", ",", "the", "retinoid", "X", "receptor", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 1, 2, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "These", "data", "demonstrate", "that", "VitD3", "induces", "CD14", "indirectly", "through", "some", "intermediary", "factor", ",", "and", "suggest", "a", "critical", "role", "for", "Sp1", "in", "this", "process", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]

[ "DNA-binding", "and", "transcriptional", "regulatory", "properties", "of", "hepatic", "leukemia", "factor", "(", "HLF", ")", "and", "the", "t", "(", "17", ";", "19", ")", "acute", "lymphoblastic", "leukemia", "chimera", "E2A-HLF", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0 ]

[ "The", "t", "(", "17", ";", "19", ")", "translocation", "in", "acute", "lymphoblastic", "leukemias", "results", "in", "creation", "of", "E2A-hepatic", "leukemia", "factor", "(", "HLF", ")", "chimeric", "proteins", "that", "contain", "the", "DNA-binding", "and", "protein", "dimerization", "domains", "of", "the", "basic", "leucine", "zipper", "(", "bZIP", ")", "protein", "HLF", "fused", "to", "a", "portion", "of", "E2A", "proteins", "with", "transcriptional", "activation", "properties", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0 ]

[ "An", "in", "vitro", "binding", "site", "selection", "procedure", "was", "used", "to", "determine", "DNA", "sequences", "preferentially", "bound", "by", "wild-type", "HLF", "and", "chimeric", "E2A-HLF", "proteins", "isolated", "from", "various", "t", "(", "17", ";", "19", ")", "-bearing", "leukemias", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "All", "were", "found", "to", "selectively", "bind", "the", "consensus", "sequence", "5'-GTTACGTAAT-3", "'", "with", "high", "affinity", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Wild-type", "and", "chimeric", "HLF", "proteins", "also", "bound", "closely", "related", "sites", "identified", "previously", "for", "bZIP", "proteins", "of", "both", "the", "proline", "-and", "acidic", "amino", "acid-rich", "(", "PAR", ")", "and", "C/EBP", "subfamilies", ";", "however", ",", "E2A-HLF", "proteins", "were", "significantly", "less", "tolerant", "of", "certain", "deviations", "from", "the", "HLF", "consensus", "binding", "site", "." ]

[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 10, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]

[ "These", "differences", "were", "directly", "attributable", "to", "loss", "of", "an", "HLF", "ancillary", "DNA-binding", "domain", "in", "all", "E2A-HLF", "chimeras", "and", "were", "further", "exacerbated", "by", "a", "zipper", "mutation", "in", "one", "isolate", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Both", "wild-type", "and", "chimeric", "HLF", "proteins", "displayed", "transcriptional", "activator", "properties", "in", "lymphoid", "and", "nonlymphoid", "cells", "on", "reporter", "genes", "containing", "HLF", "or", "C/EBP", "consensus", "binding", "sites", "." ]

[ 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 1, 0, 1, 2, 2, 2, 0 ]

[ "But", "on", "reporter", "genes", "with", "nonoptimal", "binding", "sites", ",", "their", "transcriptional", "properties", "diverged", "and", "E2A-HLF", "competitively", "inhibited", "activation", "by", "wild-type", "PAR", "proteins", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "These", "findings", "establish", "a", "spectrum", "of", "binding", "site-specific", "transcriptional", "properties", "for", "E2A-HLF", "which", "may", "preferentially", "activate", "expression", "of", "select", "subordinate", "genes", "as", "a", "homodimer", "and", "potentially", "antagonize", "expression", "of", "others", "through", "heteromeric", "interactions", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "ZAP-70", "tyrosine", "kinase", ",", "CD45", ",", "and", "T", "cell", "receptor", "involvement", "in", "UV-", "and", "H2O2-induced", "T", "cell", "signal", "transduction", "." ]

[ 9, 10, 10, 0, 9, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0 ]

[ "Several", "mammalian", "responses", "to", "UV", "irradiation", ",", "including", "the", "activation", "of", "NF-kappa", "B", ",", "are", "believed", "to", "involve", "tyrosine", "phosphorylation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "UV", "irradiation", "and", "H2O2", "treatment", "of", "T", "lymphocytes", "induce", "protein", "tyrosine", "phosphorylation", "and", "Ca2+", "signals", "similar", "to", "those", "observed", "following", "biological", "stimulation", "." ]

[ 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "have", "examined", "the", "role", "of", "cell", "surface", "molecules", "in", "these", "responses", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Normal", "T", "lymphocytes", "whose", "surface", "expression", "of", "CD3", "was", "depleted", "showed", "impaired", "UV-induced", "tyrosine", "phosphorylation", "and", "Ca2+", "signals", "." ]

[ 0, 7, 8, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Similarly", ",", "Jurkat", "T", "cell", "lines", "deficient", "in", "CD3", "or", "CD45", "expression", "also", "gave", "impaired", "UV", "responses", "." ]

[ 0, 0, 5, 6, 6, 6, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]

[ "However", ",", "all", "these", "cell", "types", "still", "gave", "strong", "Ca2+", "and", "tyrosine", "phosphorylation", "responses", "to", "H2O2", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "T", "cell", "tyrosine", "kinase", "ZAP-70", "was", "found", "to", "be", "highly", "responsive", "to", "UV", "and", "H2O2", "treatment", "." ]

[ 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "ZAP-70", "responsiveness", "to", "UV", "required", "expression", "of", "both", "CD3", "and", "CD45", ",", "whereas", "only", "CD3", "was", "required", "for", "the", "response", "to", "H2O2", "." ]

[ 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "UV-induced", "activation", "of", "NF-kappa", "B", "was", "blocked", "by", "CD3", "depletion", ",", "indicating", "the", "importance", "of", "such", "cell", "surface", "molecules", "in", "biological", "responses", "to", "UV", "." ]

[ 0, 0, 0, 9, 10, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "nonlymphoid", "cells", ",", "the", "epidermal", "growth", "factor", "receptor", "displayed", "increased", "tyrosine", "phosphorylation", "within", "seconds", "of", "UV", "irradiation", "." ]

[ 0, 7, 8, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "These", "results", "suggest", "that", "UV-induced", "signal", "transduction", "is", "mediated", "via", "cell", "surface", "receptors", "that", "normally", "respond", "to", "biological", "stimulation", ",", "whereas", "H2O2", "is", "able", "to", "partially", "bypass", "this", "requirement", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Inhibition", "of", "the", "differentiation", "of", "human", "myeloid", "cell", "lines", "by", "redox", "changes", "induced", "through", "glutathione", "depletion", "." ]

[ 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "have", "investigated", "the", "effect", "of", "redox", "changes", "in", "vivo", "on", "the", "differentiation", "of", "two", "human", "myeloid", "cell", "lines", ",", "HL-60", "and", "KG-1", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 5, 0, 5, 0 ]

[ "The", "glutathione-depleting", "agent", "diethyl", "maleate", "(", "DEM", ")", "prevented", "the", "development", "of", "differentiated", "features", "in", "response", "to", "phorbol", "esters", ",", "including", "adherence", "of", "the", "cells", "to", "plastic", "surfaces", "and", "repression", "of", "the", "myeloperoxidase", "and", "CD34", "genes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 0 ]

[ "Moreover", ",", "DEM", "abolished", "phorbol", "12-myristate", "13-acetate-induced", "activation", "of", "the", "transcription", "factors", "AP-1", "and", "Egr-1", ",", "suggesting", "that", "inhibition", "of", "differentiation", "may", "be", "due", ",", "at", "least", "in", "part", ",", "to", "redox", "modifications", "of", "these", "proteins", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Lipopolysaccharide", "induction", "of", "tissue", "factor", "gene", "expression", "in", "monocytic", "cells", "is", "mediated", "by", "binding", "of", "c-Rel/p65", "heterodimers", "to", "a", "kappa", "B-like", "site", "." ]

[ 0, 0, 0, 1, 2, 2, 0, 0, 7, 8, 0, 0, 0, 0, 0, 9, 10, 0, 0, 1, 2, 2, 0 ]

[ "Exposure", "of", "monocytic", "cells", "to", "bacterial", "lipopolysaccharide", "(", "LPS", ")", "activates", "the", "NF-kappa", "B/Rel", "family", "of", "proteins", "and", "leads", "to", "the", "rapid", "induction", "of", "inflammatory", "gene", "products", ",", "including", "tissue", "factor", "(", "TF", ")", "." ]

[ 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0 ]

[ "TF", "is", "the", "primary", "cellular", "initiator", "of", "the", "coagulation", "protease", "cascades", "." ]

[ 9, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]

[ "Here", "we", "report", "the", "characterization", "of", "a", "nuclear", "complex", "from", "human", "monocytic", "cells", "that", "bound", "to", "a", "kappa", "B-like", "site", ",", "5'-CGGAGTTTCC-3", "'", ",", "in", "the", "5'-flanking", "region", "of", "the", "human", "TF", "gene", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0 ]

[ "This", "nuclear", "complex", "was", "activated", "by", "LPS", "with", "kinetics", "that", "preceded", "induction", "of", "the", "TF", "gene", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]

[ "In", "vitro", "binding", "studies", "demonstrated", "that", "the", "TF", "site", "bound", "translated", "c-Rel", "and", "p65", "homodimers", "but", "not", "p50/p65", "heterodimers", "or", "p50", "homodimers", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 9, 0, 9, 10, 0, 0, 9, 10, 0, 9, 10, 0 ]

[ "Base-pair", "substitutions", "in", "the", "TF", "site", "indicated", "that", "the", "presence", "of", "a", "cytosine", "at", "position", "1", "precluded", "binding", "of", "NF-kappa", "B", "." ]

[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "In", "fact", ",", "under", "low-ionic-strength", "conditions", ",", "the", "TF", "complex", "did", "not", "migrate", "with", "translated", "p50/p65", "dimers", "but", "instead", "comigrated", "with", "c-Rel/p65", "dimers", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0 ]

[ "Antibodies", "against", "the", "NF-kappa", "B", "and", "Rel", "proteins", "and", "UV", "cross-linking", "studies", "revealed", "the", "presence", "of", "c-Rel", "and", "p65", "and", "the", "absence", "of", "p50", "in", "the", "TF", "complex", "and", "further", "showed", "that", "c-Rel/p65", "heterodimers", "selectively", "bound", "to", "the", "TF", "kappa", "B-like", "site", "." ]

[ 0, 0, 0, 9, 10, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 9, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]

[ "Functional", "studies", "indicated", "that", "the", "TF", "site", "conferred", "LPS", "inducibility", "on", "a", "heterologous", "promoter", "and", "was", "transactivated", "by", "c-Rel", "or", "p65", "." ]

[ 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0 ]

[ "Taken", "together", ",", "our", "results", "demonstrated", "that", "binding", "of", "c-Rel/p65", "heterodimers", "to", "a", "novel", "kappa", "B-like", "site", "mediated", "LPS", "induction", "of", "TF", "gene", "expression", "in", "monocytic", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 1, 2, 0, 0, 7, 8, 0 ]

[ "Inhibition", "of", "T", "cell", "activation", "by", "the", "extracellular", "matrix", "protein", "tenascin", "." ]

[ 0, 0, 7, 8, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "Tenascin", "(", "TN", ")", "is", "an", "extracellular", "matrix", "protein", "that", "is", "expressed", "widely", "in", "the", "fetus", "and", "sparingly", "in", "the", "adult", ",", "but", "reappears", "at", "high", "levels", "in", "certain", "areas", "of", "tissue", "insult", "such", "as", "tumor", "matrices", "and", "sites", "of", "wound", "healing", "." ]

[ 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "show", "here", "that", "soluble", "TN", "inhibits", "proliferation", "of", "human", "T", "cells", "in", "response", "to", "alpha", "CD3", "Ab", "co-immobilized", "with", "the", "extracellular", "matrix", "protein", "fibronectin", "(", "FN", ")", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 7, 8, 8, 0, 0, 0, 9, 10, 10, 0, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0 ]

[ "TN", "also", "inhibits", "proliferation", "driven", "by", "alpha", "CD3/IL-2", "or", "by", "phorbol", "ester/IL-2", ",", "and", "it", "prevents", "high", "level", "induction", "of", "IL-2R", "." ]

[ 9, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "The", "presence", "of", "TN", "in", "culture", "medium", "does", "not", "detectably", "alter", "the", "pattern", "of", "tyrosine", "phosphorylation", "resulting", "from", "T", "cell", "triggering", "with", "alpha", "CD3", ",", "but", "at", "later", "time", "points", "prevents", "the", "appearance", "of", "functional", "NF-AT1", "transcription", "factor", "complexes", "in", "T", "cell", "nuclear", "extracts", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 7, 8, 0, 0, 0 ]

[ "These", "findings", "are", "consistent", "with", "the", "postulated", "role", "for", "TN", "as", "a", "natural", "antagonist", "to", "FN", "action", ",", "and", "suggest", "that", "T", "cell", "responses", "occurring", "at", "tissue", "sites", "in", "which", "TN", "is", "expressed", "could", "be", "influenced", "by", "its", "presence", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Human", "immunodeficiency", "virus", "type", "1", "Nef", "protein", "down-regulates", "transcription", "factors", "NF-kappa", "B", "and", "AP-1", "in", "human", "T", "cells", "in", "vitro", "after", "T-cell", "receptor", "stimulation", "." ]

[ 9, 10, 10, 10, 10, 10, 10, 0, 9, 10, 10, 10, 0, 9, 0, 7, 8, 8, 0, 0, 0, 9, 10, 0, 0 ]

[ "Human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "negative", "factor", "(", "Nef", ")", "has", "been", "shown", "to", "down-regulate", "the", "transcription", "factors", "NF-kappa", "B", "and", "AP-1", "in", "vitro", "." ]

[ 9, 10, 10, 10, 10, 10, 10, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0, 0 ]

[ "To", "define", "the", "mechanism", "of", "action", "of", "the", "Nef", "protein", ",", "the", "signal", "transduction", "pathways", "which", "may", "be", "affected", "in", "T", "cells", "by", "constitutive", "expression", "of", "the", "nef", "gene", "were", "examined", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0 ]

[ "Stimulation", "of", "T", "cells", "with", "tumor", "necrosis", "factor", ",", "interleukin-1", ",", "or", "lipopolysaccharide", "resulted", "in", "the", "recruitment", "of", "transcriptional", "factors", "to", "a", "similar", "level", "whether", "or", "not", "the", "cells", "expressed", "the", "nef", "gene", "." ]

[ 0, 0, 7, 8, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]

[ "On", "the", "other", "hand", ",", "stimulation", "of", "T", "cells", "by", "mitogens", "or", "antibodies", "to", "the", "T-cell", "receptor", "(", "TCR", ")", "-", "CD3", "complex", "resulted", "in", "the", "down-regulation", "of", "transcriptional", "factors", "NF-kappa", "B", "and", "AP-1", "in", "cells", "expressing", "the", "nef", "gene", "compared", "with", "cells", "not", "expressing", "the", "nef", "gene", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]

[ "Because", "the", "Nef", "protein", "does", "not", "affect", "the", "surface", "expression", "of", "the", "CD3", "-TCR", "complex", ",", "we", "conclude", "that", "the", "Nef", "protein", "down-regulates", "the", "transcriptional", "factors", "NF-kappa", "B", "and", "AP-1", "in", "T", "cells", "in", "vitro", "through", "an", "effect", "on", "the", "TCR-dependent", "signal", "transduction", "pathway", "." ]

[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0, 9, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Effects", "of", "alpha-lipoic", "acid", "and", "dihydrolipoic", "acid", "on", "expression", "of", "proto-oncogene", "c-fos", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]

[ "The", "transcription", "factor", "AP-1", "is", "an", "important", "human", "mediator", "of", "the", "cellular", "response", "to", "serum", ",", "growth", "factors", ",", "and", "phorbol", "esters", "such", "as", "12-O-tetradecanoyl-phorbol-13", "acetate", "(", "TPA", ")", "." ]

[ 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "AP-1", "complex", "consists", "of", "distinct", "protein", "heterodimers", "encoded", "by", "the", "proto-oncogene", "c-fos", "and", "c-jun", "mRNA", "whose", "gene", "expression", "can", "be", "induced", "by", "TPA", ",", "cyclic", "AMP", "and", "growth", "factors", "." ]

[ 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Recent", "findings", "suggest", "an", "involvement", "of", "reactive", "oxygen", "species", "in", "the", "pathway", "of", "TPA", "and", "protein", "kinase", "C", "leading", "to", "expression", "of", "c-fos", "and", "c-jun", "mRNA", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 3, 0, 3, 4, 0 ]

[ "To", "investigate", "the", "role", "of", "reactive", "oxygen", "species", "we", "studied", "the", "effects", "of", "alpha-lipoic", "acid", "and", "dihydrolipoic", "acid", "(", "natural", "thiol", "antioxidants", ")", "on", "the", "expression", "of", "c-fos", "mRNA", "in", "human", "Jurkat", "T", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 5, 6, 6, 6, 0 ]

[ "When", "cells", "were", "preincubated", "with", "dihydrolipoic", "acid", "(", "0.2", "mM", ")", "the", "expression", "of", "c-fos", "mRNA", "was", "suppressed", "at", "30", "min", "after", "stimulation", "of", "TPA", "(", "0.5", "microM", ")", "whereas", "in", "the", "case", "of", "preincubation", "of", "alpha-lipoic", "acid", "(", "0.2", "microM", ")", ",", "the", "expression", "was", "enhanced", "at", "30", "min", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "These", "studies", "support", "the", "idea", "that", "superoxide", "anion", "radical", "plays", "a", "role", "in", "the", "expression", "of", "c-fos", "mRNA", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 3, 4, 0 ]

[ "Appraisal", "of", "potential", "therapeutic", "index", "of", "antioxidants", "on", "the", "basis", "of", "their", "in", "vitro", "effects", "on", "HIV", "replication", "in", "monocytes", "and", "interleukin", "2", "-induced", "lymphocyte", "proliferation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 9, 10, 0, 7, 0, 0 ]

[ "Antioxidant", "molecules", "have", "been", "suggested", "to", "be", "of", "therapeutic", "value", "in", "the", "treatment", "of", "HIV-infected", "patients", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "To", "evaluate", "this", "possibility", ",", "we", "examined", "in", "vitro", "the", "effects", "of", "two", "types", "of", "antioxidant", "molecules", "in", "terms", "of", "inhibition", "of", "HIV", "replication", "in", "monocytes", ",", "one", "of", "the", "main", "reservoirs", "of", "HIV", ",", "and", "also", "in", "terms", "of", "modulation", "of", "the", "immune", "competence", "as", "measured", "by", "PBMC", "proliferation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0 ]

[ "We", "tested", "the", "effects", "of", "BHA", ",", "a", "phenolic", ",", "lipid-soluble", ",", "chain-breaking", "antioxidant", ",", "and", "NAC", ",", "a", "known", "glutathione", "precursor", "with", "some", "direct", "free-radical", "scavenging", "properties", "as", "well", ",", "on", "the", "regulation", "of", "HIV-1", "expression", "in", "latently", "infected", "U1", "cells", "and", "in", "productively", "and", "chronically", "infected", "U937", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]

[ "Both", "antioxidants", "inhibited", "TNF", "-or", "PMA-induced", "NF-kappa", "B", "activity", "in", "U1", "cells", ",", "as", "well", "as", "the", "sustained", "NF-kappa", "B", "activity", "permanently", "induced", "by", "the", "virus", "itself", "in", "chronically", "HIV-infected", "U937", "cells", "." ]

[ 0, 0, 0, 9, 0, 0, 9, 10, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]

[ "This", "resulted", "in", "only", "a", "partial", "inhibition", "of", "TNF", "-or", "PMA-", "induced", "HIV", "replication", "in", "U1", "cells", ",", "and", "no", "detectable", "effect", "on", "HIV", "replication", "in", "chronically", "infected", "U937", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]

[ "This", "may", "be", "the", "first", "limitation", "to", "potential", "antiviral", "effects", "of", "antioxidant", "therapies", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Another", "limitation", "is", "that", "antioxidant", "concentrations", "high", "enough", "to", "block", "NK-kappa", "B", "activation", "were", "shown", "to", "have", "a", "suppressive", "effect", "on", "immune", "functions", "in", "vitro", ",", "because", "NAC", "and", "BHA", "blocked", "IL-2-induced", "PBMC", "proliferation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0 ]

[ "These", "data", "warrant", "prudence", "in", "the", "design", "of", "antioxidant-based", "therapies", "aimed", "at", "suppressing", "HIV", "replication", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Isolation", "and", "characterization", "of", "gelatinase", "granules", "from", "human", "neutrophils", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 7, 8, 0 ]

[ "We", "recently", "confirmed", "the", "existence", "of", "gelatinase", "granules", "as", "a", "subpopulation", "of", "peroxidase-negative", "granules", "by", "double-labeling", "immunogold", "electron", "microscopy", "on", "intact", "cells", "and", "by", "subcellular", "fractionation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Further", "characterization", "of", "gelatinase", "granules", "has", "been", "hampered", "by", "poor", "separation", "of", "specific", "and", "gelatinase", "granules", "on", "both", "two-layer", "Percoll", "gradients", "and", "sucrose", "gradients", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "have", "developed", "a", "three-layer", "Percoll", "density", "gradient", "that", "allows", "separation", "of", "the", "different", "granules", "and", "vesicles", "from", "human", "neutrophils", ";", "in", "particular", ",", "it", "allows", "separation", "of", "specific", "and", "gelatinase", "granules", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "This", "allows", "us", "to", "characterize", "these", "two", "granule", "populations", "with", "regard", "to", "their", "content", "of", "membrane", "proteins", ",", "which", "become", "incorporated", "into", "the", "plasma", "membrane", "during", "exocytosis", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "found", "that", "gelatinase", "granules", ",", "defined", "as", "peroxidase-negative", "granules", "containing", "gelatinase", "but", "lacking", "lactoferrin", ",", "contain", "50", "%", "of", "total", "cell", "gelatinase", ",", "with", "the", "remaining", "residing", "in", "specific", "granules", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Furthermore", ",", "we", "found", "that", "20", "%", "to", "25", "%", "of", "both", "the", "adhesion", "protein", "Mac-1", "and", "the", "NADPH-oxidase", "component", "cytochrome", "b558", "is", "localized", "in", "gelatinase", "granules", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 9, 10, 0, 0, 0, 9, 0, 0 ]

[ "Although", "no", "qualitative", "difference", "was", "observed", "between", "specific", "granules", "and", "gelatinase", "granules", "with", "respect", "to", "cytochrome", "b558", "and", "Mac-1", ",", "stimulation", "of", "the", "neutrophil", "with", "FMLP", "resulted", "in", "a", "selective", "mobilization", "of", "the", "least", "dense", "peroxidase-negative", "granules", ",", "ie", ",", "gelatinase", "granules", ",", "which", ",", "in", "concert", "with", "secretory", "vesicles", ",", "furnish", "the", "plasma", "membrane", "with", "Mac-1", "and", "cytochrome", "b558", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 7, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 0 ]

[ "This", "shows", "that", "gelatinase", "granules", "are", "functionally", "important", "relative", "to", "specific", "granules", "in", "mediating", "early", "inflammatory", "responses", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Regulation", "of", "interleukin-2", "receptor", "alpha", "chain", "expression", "and", "nuclear", "factor.kappa", "B", "activation", "by", "protein", "kinase", "C", "in", "T", "lymphocytes", "." ]

[ 0, 0, 9, 10, 10, 10, 0, 0, 9, 10, 10, 0, 0, 9, 10, 10, 0, 7, 8, 0 ]

[ "Autocrine", "role", "of", "tumor", "necrosis", "factor", "alpha", "." ]

[ 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "The", "regulation", "of", "interleukin-2", "receptor", "alpha", "chain", "(", "IL-2R", "alpha", ")", "expression", "and", "nuclear", "factor", "(", "NF", ")", "activation", "by", "protein", "kinase", "C", "(", "PKC", ")", "in", "resting", "T", "cells", ",", "has", "been", "studied", "." ]

[ 0, 0, 0, 9, 10, 10, 10, 0, 9, 10, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0 ]

[ "Treatment", "of", "human", "resting", "T", "cells", "with", "phorbol", "esters", "strongly", "induced", "the", "expression", "of", "IL-2R", "alpha", "and", "the", "activation", "of", "NF.kappa", "B", "." ]

[ 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0 ]

[ "This", "activation", "was", "due", "to", "the", "translocation", "of", "p65", "and", "c-Rel", "NF.kappa", "B", "proteins", "from", "cytoplasmic", "stores", "to", "the", "nucleus", ",", "where", "they", "bound", "the", "kappa", "B", "sequence", "of", "the", "IL-2R", "alpha", "promoter", "either", "as", "p50.p65", "or", "as", "p50.c-Rel", "heterodimers", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 2, 0, 0, 9, 0, 0, 9, 0, 0 ]

[ "Interestingly", ",", "all", "of", "those", "events", "were", "largely", "indirect", "and", "mediated", "by", "endogenously", "secreted", "tumor", "necrosis", "factor", "alpha", "(", "TNF", "alpha", ")", ",", "as", "they", "were", "strongly", "inhibited", "by", "a", "neutralizing", "anti-TNF", "alpha", "monoclonal", "antibody", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "Furthermore", ",", "cyclosporin", "A", ",", "which", "blocked", "TNF", "alpha", "production", "induced", "by", "PKC", ",", "strongly", "inhibited", "IL-2R", "alpha", "and", "NF.kappa", "B", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 9, 0, 0, 0, 9, 10, 0, 9, 10, 0, 0 ]

[ "The", "addition", "of", "either", "TNF", "alpha", "or", "IL-2", "partially", "recovered", "cyclosporin", "A-induced", "IL-2R", "alpha", "inhibition", ",", "but", "only", "TNF", "alpha", "completely", "recovered", "NF.kappa", "B", "activation", "." ]

[ 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 10, 0, 0, 9, 10, 0, 0 ]

[ "Those", "results", "indicate", "that", ",", "in", "resting", "T", "cells", ",", "PKC", "activation", "has", "only", "a", "triggering", "role", ",", "whereas", "the", "endogenously", "secreted", "TNF", "alpha", "plays", "an", "essential", "role", "in", "the", "quantitative", "control", "of", "the", "expression", "of", "IL-2R", "alpha", "chain", "or", "NF.kappa", "B", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 10, 0, 0 ]

[ "Superantigens", "activate", "HIV-1", "gene", "expression", "in", "monocytic", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "Binding", "of", "superantigens", "to", "MHC", "class", "II", "molecules", "results", "in", "transduction", "of", "biochemical", "signals", "leading", "to", "cellular", "activation", "and", "gene", "expression", "." ]

[ 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "demonstrate", "that", "the", "staphylococcal", "superantigens", "toxic", "shock", "syndrome", "toxin-1", "(", "TSST-1", ")", "and", "staphylococcal", "enterotoxin", "A", "(", "SEA", ")", "activate", "HIV-1-LTR", "-driven", "transcription", "of", "chloramphenicol", "acetyl", "transferase", "in", "the", "human", "monocytic", "cell", "line", "THP-1", "." ]

[ 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0, 9, 0, 0, 9, 10, 10, 0, 9, 0, 0, 1, 0, 0, 0, 9, 10, 10, 0, 0, 5, 6, 6, 6, 6, 0 ]