app.py

from transformers import pipeline
from rcsbsearchapi import TextQuery, AttributeQuery, Query
from rcsbsearchapi.search import Sort, SequenceQuery
import os
from dotenv import load_dotenv
from shiny import App, render, ui, reactive
import pandas as pd
import warnings
import re
from  UniprotKB_P_Sequence_RCSB_API_test import ProteinQuery, ProteinSearchEngine
import plotly.graph_objects as go
from shinywidgets import output_widget, render_widget
import requests
import asyncio
warnings.filterwarnings('ignore')

# Load environment variables from .env file
load_dotenv()

# os.environ["TRANSFORMERS_CACHE"] = "./transformers_cache"
# os.makedirs("./transformers_cache", exist_ok=True)

class PDBSearchAssistant:
    def __init__(self, model_name="google/flan-t5-large"):
        # Set up HuggingFace pipeline with better model
        self.pipe = pipeline(
            "text2text-generation",
            model=model_name,
            max_new_tokens=512,
            temperature=0.3,
            torch_dtype="auto",
            device="cpu"
        )
        
        self.prompt_template = """
            Extract specific search parameters from the protein-related query:
            1. Protein name or type
            2. Resolution cutoff (in Å)
            3. Protein sequence information
            4. Specific PDB ID
            5. Experimental method (X-RAY, EM, NMR)
            6. Organism/Species information

            Format:
            Protein: [protein name or type]
            Organism: [organism/species if mentioned]
            Resolution: [maximum resolution in Å, if mentioned]
            Sequence: [any sequence mentioned]
            PDB_ID: [specific PDB ID if mentioned]
            Method: [experimental method if mentioned]

            Examples:
            Query: "Find human insulin structures with X-ray better than 2.5Å resolution"
            Protein: insulin
            Organism: human
            Resolution: 2.5
            Sequence: none
            PDB_ID: none
            Method: X-RAY

            Query: "Get sequence of PDB ID 8ET6"
            Protein: none
            Organism: none
            Resolution: none
            Sequence: none
            PDB_ID: 8ET6
            Method: none

            Query: "Sequence of 7BZ5"
            Protein: none
            Organism: none
            Resolution: none
            Sequence: none
            PDB_ID: 7BZ5
            Method: none

            Query: "7BZ5"
            Protein: none
            Organism: none
            Resolution: none
            Sequence: none
            PDB_ID: 7BZ5
            Method: none

            Query: "6KAO"
            Protein: none
            Organism: none
            Resolution: none
            Sequence: none
            PDB_ID: 6KAO
            Method: none
            
            Query: "Find structures containing sequence MNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYKNL"
            Protein: none
            Organism: none
            Resolution: none
            Sequence: MNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYKNL
            PDB_ID: none
            Method: none

            Now analyze:
            Query: {query}
            """

    def search_pdb(self, query):
        try:
            # Get search parameters from LLM
            formatted_prompt = self.prompt_template.format(query=query)
            response = self.pipe(formatted_prompt)[0]['generated_text']
            print("Generated parameters:", response)
            
            # Parse LLM response
            resolution_limit = None
            pdb_id = None
            sequence = None
            method = None
            has_resolution_query = False
            resolution_direction = "less"
            
            # Check if query contains resolution-related terms
            resolution_terms = {
                'better': 'less',
                'best': 'less',
                'highest': 'less',
                'good': 'less',
                'fine': 'less',
                'worse': 'greater',
                'worst': 'greater',
                'lowest': 'greater',
                'poor': 'greater',
                'resolution': None,
                'å': None,
                'angstrom': None,
                'than': None,
                'under': 'less',
                'below': 'less',
                'above': 'greater',
                'over': 'greater'
            }
            
            # Check if the original query mentions resolution
            query_lower = query.lower()
            
            # Determine resolution direction from query
            for term, direction in resolution_terms.items():
                if term in query_lower:
                    has_resolution_query = True
                    if direction:  # if not None
                        resolution_direction = direction
            
            # Also check for numerical values with Å
            if re.search(r'\d+\.?\d*\s*å?', query_lower):
                has_resolution_query = True
            
            # Clean and parse LLM response
            for line in response.split('\n'):
                if 'Resolution:' in line:
                    value = line.split('Resolution:')[1].strip()
                    if value.lower() not in ['none', 'n/a'] and has_resolution_query:
                        try:
                            # Extract just the number
                            res_value = ''.join(c for c in value if c.isdigit() or c == '.')
                            resolution_limit = float(res_value)
                        except ValueError:
                            pass
                elif 'Method:' in line:
                    value = line.split('Method:')[1].strip()
                    if value.lower() not in ['none', 'n/a']:
                        method = value.upper()
                elif 'Sequence:' in line:
                    value = line.split('Sequence:')[1].strip()
                    if value.lower() not in ['none', 'n/a']:
                        sequence = value
                elif 'PDB_ID:' in line:
                    value = line.split('PDB_ID:')[1].strip()
                    if value.lower() not in ['none', 'n/a']:
                        pdb_id = value
            
            # Build search query
            queries = []
            
            # Check if the query contains a protein sequence pattern
            # Check for amino acid sequence (minimum 25 residues)
            query_words = query.split()
            for word in query_words:
                # Check if the word consists of valid amino acid letters
                if (len(word) >= 25 and  # minimum 25 residues requirement
                    all(c in 'ACDEFGHIKLMNPQRSTVWY' for c in word.upper()) and
                    sum(c.isupper() for c in word) / len(word) > 0.8):
                    sequence = word
                    break
            
            # If sequence is found, use SequenceQuery
            if sequence:
                if len(sequence) < 25:
                    print("Warning: Sequence must be at least 25 residues long. Skipping sequence search.")
                    sequence = None
                else:
                    print(f"Adding sequence search with identity 100% for sequence: {sequence}")
                    sequence_query = SequenceQuery(
                        sequence,
                        identity_cutoff=1.0,  # 100% identity
                        evalue_cutoff=1,
                        sequence_type="protein"
                    )
                    queries.append(sequence_query)
            # If no sequence, proceed with text search
            else:
                # Clean the original query and add text search
                clean_query = query.lower()
                
                # Remove resolution numbers and terms if they exist
                if has_resolution_query:
                    clean_query = re.sub(r'\d+\.?\d*\s*å?', '', clean_query)
                    for term in resolution_terms:
                        clean_query = clean_query.replace(term, '')
                
                # Clean up extra spaces and trim
                clean_query = ' '.join(clean_query.split())
                
                print("Cleaned query:", clean_query)
                
                # Add text search if query is not empty
                if clean_query.strip():
                    text_query = AttributeQuery(
                        attribute="struct.title",
                        operator="contains_phrase",
                        value=clean_query
                    )
                    queries.append(text_query)
            
            # Add resolution filter if specified
            if resolution_limit and has_resolution_query:
                operator = "less_or_equal" if resolution_direction == "less" else "greater_or_equal"
                print(f"Adding resolution filter: {operator} {resolution_limit}Å")
                resolution_query = AttributeQuery(
                    attribute="rcsb_entry_info.resolution_combined",
                    operator=operator,
                    value=resolution_limit
                )
                queries.append(resolution_query)
            
            # Add PDB ID search if specified
            if pdb_id:
                print(f"Searching for specific PDB ID: {pdb_id}")
                id_query = AttributeQuery(
                    attribute="rcsb_id",
                    operator="exact_match",
                    value=pdb_id.upper()
                )
                queries = [id_query]  # Override other queries for direct PDB ID search
            
            # Add experimental method filter if specified
            if method:
                print(f"Adding experimental method filter: {method}")
                method_query = AttributeQuery(
                    attribute="exptl.method",
                    operator="exact_match",
                    value=method
                )
                queries.append(method_query)
            
            # Combine queries with AND operator
            if queries:
                final_query = queries[0]
                for q in queries[1:]:
                    final_query = final_query & q
                
                print("Final query:", final_query)
                
                # Execute search
                session = final_query.exec()
                results = []
                
                # Process results with additional information
                search_engine = ProteinSearchEngine()
                
                try:
                    for entry in session:
                        try:
                            # PDB ID 추출 방식 개선
                            if isinstance(entry, dict):
                                pdb_id = entry.get('identifier')
                            elif hasattr(entry, 'identifier'):
                                pdb_id = entry.identifier
                            else:
                                pdb_id = str(entry)
                            
                            pdb_id = pdb_id.upper()  # PDB ID는 항상 대문자
                            
                            if not pdb_id or len(pdb_id) != 4:  # PDB ID는 항상 4자리
                                continue
                                
                            # RCSB PDB REST API를 직접 사용하여 구조 정보 가져오기
                            structure_url = f"https://data.rcsb.org/rest/v1/core/entry/{pdb_id}"
                            response = requests.get(structure_url)
                            
                            if response.status_code != 200:
                                continue
                                
                            structure_data = response.json()
                            
                            # 결과 구성
                            result = {
                                'PDB ID': pdb_id,
                                'Resolution': f"{structure_data.get('rcsb_entry_info', {}).get('resolution_combined', [0.0])[0]:.2f}Å",
                                'Method': structure_data.get('exptl', [{}])[0].get('method', 'Unknown'),
                                'Title': structure_data.get('struct', {}).get('title', 'N/A'),
                                'Release Date': structure_data.get('rcsb_accession_info', {}).get('initial_release_date', 'N/A')
                            }
                            
                            results.append(result)
                            
                            # Limit to top 10 results
                            if len(results) >= 10:
                                break
                                
                        except Exception as e:
                            print(f"Error processing entry: {str(e)}")
                            continue
                            
                except Exception as e:
                    print(f"Error processing results: {str(e)}")
                    print(f"Error type: {type(e)}")
                    
                print(f"Found {len(results)} structures")
                return results
                
            return []
            
        except Exception as e:
            print(f"Error during search: {str(e)}")
            print(f"Error type: {type(e)}")
            return []

    def get_sequences_by_pdb_id(self, pdb_id):
        """Get sequences for all chains in a PDB structure"""
        try:
            # ProteinSearchEngine 인스턴스 생성
            search_engine = ProteinSearchEngine()
            
            # ProteinQuery 객체 생성 (resolution limit은 높게 설정하여 모든 결과 포함)
            query = ProteinQuery(
                name=pdb_id,
                max_resolution=100.0  # 높은 값으로 설정하여 모든 구조 포함
            )
            
            # 검색 실행
            results = search_engine.search(query)
            
            if not results:
                return []
                
            sequences = []
            # 결과에서 sequence 정보 추출
            for structure in results:
                if structure.pdb_id.upper() == pdb_id.upper():
                    chain_info = {
                        'chain_id': 'ALL',  # 체인 정보는 통합
                        'entity_id': '1',
                        'description': structure.title,
                        'sequence': structure.sequence,
                        'length': len(structure.sequence),
                        'resolution': structure.resolution,
                        'method': structure.method,
                        'release_date': structure.release_date
                    }
                    sequences.append(chain_info)
                    break  # 정확한 PDB ID 매치를 찾으면 중단
            
            # 결과가 없으면 직접 API 호출 시도
            if not sequences:
                print(f"No results found using ProteinSearchEngine, trying direct API call...")
                return self._get_sequences_by_direct_api(pdb_id)
                
            return sequences
            
        except Exception as e:
            print(f"Error in ProteinSearchEngine search for PDB ID {pdb_id}: {str(e)}")
            # 에러 발생 시 직접 API 호출로 폴백
            return self._get_sequences_by_direct_api(pdb_id)

    def _get_sequences_by_direct_api(self, pdb_id):
        """Fallback method using direct API calls"""
        # 기존의 get_sequences_by_pdb_id 메소드 내용을 여기로 이동
        try:
            url = f"https://data.rcsb.org/rest/v1/core/polymer_entity_instances/{pdb_id}"
            response = requests.get(url)
            
            if response.status_code != 200:
                return []
                
            chains_data = response.json()
            sequences = []
            
            for chain_id in chains_data.keys():
                entity_id = chains_data[chain_id].get('rcsb_polymer_entity_instance_container_identifiers', {}).get('entity_id')
                
                if entity_id:
                    entity_url = f"https://data.rcsb.org/rest/v1/core/polymer_entity/{pdb_id}/{entity_id}"
                    entity_response = requests.get(entity_url)
                    
                    if entity_response.status_code == 200:
                        entity_data = entity_response.json()
                        sequence = entity_data.get('entity_poly', {}).get('pdbx_seq_one_letter_code', '')
                        description = entity_data.get('rcsb_polymer_entity', {}).get('pdbx_description', 'N/A')
                        
                        chain_info = {
                            'chain_id': chain_id,
                            'entity_id': entity_id,
                            'description': description,
                            'sequence': sequence,
                            'length': len(sequence)
                        }
                        sequences.append(chain_info)
            
            return sequences
            
        except Exception as e:
            print(f"Error in direct API call for PDB ID {pdb_id}: {str(e)}")
            return []

    def process_query(self, query):
        """Process query and return results"""
        try:
            # Get search parameters from LLM
            formatted_prompt = self.prompt_template.format(query=query)
            response = self.pipe(formatted_prompt)[0]['generated_text']
            print("Generated parameters:", response)
            
            # Parse LLM response for PDB ID
            pdb_id = None
            for line in response.split('\n'):
                if 'PDB_ID:' in line:
                    value = line.split('PDB_ID:')[1].strip()
                    if value.lower() not in ['none', 'n/a']:
                        pdb_id = value.upper()
                        break
            
            # Check if query is asking for sequence
            sequence_keywords = ['sequence', 'seq']
            is_sequence_query = any(keyword in query.lower() for keyword in sequence_keywords)
            
            if is_sequence_query and pdb_id:
                # Get sequences for the PDB ID
                sequences = self.get_sequences_by_pdb_id(pdb_id)
                return {
                    "type": "sequence",
                    "results": sequences
                }
            
            # If not a sequence query or no PDB ID found, proceed with normal structure search
            return {
                "type": "structure",
                "results": self.search_pdb(query)
            }
            
        except Exception as e:
            print(f"Error processing query: {str(e)}")
            return {"type": "structure", "results": []}

def pdbsummary(name):

    search_engine = ProteinSearchEngine()

    query = ProteinQuery(
        name,
        max_resolution= 5.0
    )

    results = search_engine.search(query)

    answer = ""
    for i, structure in enumerate(results, 1):
        answer += f"\n{i}. PDB ID : {structure.pdb_id}\n"
        answer += f"\nResolution : {structure.resolution:.2f} A \n"
        answer += f"Method : {structure.method}\n Title : {structure.title}\n"
        answer += f"Release Date : {structure.release_date}\n Sequence length: {len(structure.sequence)} aa\n"
        answer += f"    Sequence:\n {structure.sequence}\n"

    return answer


def render_html(pdb_id, chain="A"):
    if pdb_id is None or chain is None:
        return ""
    html_content = f"""
    <html>
    <header>
        <script src="https://3Dmol.org/build/3Dmol-min.js"></script>
        <script src="https://3Dmol.org/build/3Dmol.ui-min.js"></script>
    </header>
    <body>
        <div style="height: 400px; position: relative;" class="viewer_3Dmoljs"
             data-pdb="{pdb_id}"
             data-backgroundalpha="0.0"
             data-style="cartoon:color=white"
             data-select1="chain:{chain}"
             data-zoomto="chain:{chain}"
             data-style1="cartoon:color=spectrum"
             data-spin="axis:y;speed:0.2">
        </div>
    </body>
    </html>
    """
    iframe = f"""
    <iframe style="width: 100%; height: 480px; border: none;" 
            srcdoc='{html_content}'>
    </iframe>
    """
    return iframe


def create_interactive_table(df):
    if df.empty:
        return go.Figure()
    
    # Reorder columns
    column_order = ['PDB ID', 'Resolution', 'Method', 'Title', 'Release Date']
    df = df[column_order]
    
    # Release Date 형식 변경 (YYYY-MM-DD)
    df['Release Date'] = pd.to_datetime(df['Release Date']).dt.strftime('%Y-%m-%d')
    
    # Create interactive table
    table = go.Figure(data=[go.Table(
        header=dict(
            values=list(df.columns),
            fill_color='paleturquoise',
            align='center',  # 헤더 중앙 정렬
            font=dict(size=16),  # 헤더 글자 크기 증가
        ),
        cells=dict(
            values=[
                [f'<a href="https://www.rcsb.org/structure/{cell}">{cell}</a>' 
                 if i == 0 else cell
                 for cell in df[col]] 
                for i, col in enumerate(df.columns)
            ],
            align='center',  # 셀 내용 중앙 정렬
            font=dict(size=15),  # 셀 글자 크기 증가
            height=35  # 셀 높이 증가
        ),
        columnwidth=[80, 80, 100, 400, 100],
        customdata=[['html'] * len(df) if i == 0 else [''] * len(df)
                   for i in range(len(df.columns))],
        hoverlabel=dict(bgcolor='white')
    )])
    
    # Update table layout
    table.update_layout(
        margin=dict(l=20, r=20, t=20, b=20),
        height=450,  # 테이블 전체 높이 증가
        autosize=True
    )
    
    return table

# Simplified Shiny app UI definition
app_ui = ui.page_fluid(
    ui.tags.head(
        ui.tags.style("""
            .container-fluid {
                max-width: 1200px;
                margin: 0 auto;
                padding: 20px;
            }
            .table a {
                color: #0d6efd;
                text-decoration: none;
            }
            .table a:hover {
                color: #0a58ca;
                text-decoration: underline;
            }
            .shiny-input-container {
                max-width: 100%;
                margin: 0 auto;
            }
            #query {
                height: 100px;
                font-size: 16px;
                padding: 15px;
                width: 80%;
                margin: 0 auto;
                display: block;
            }
            .content-wrapper {
                text-align: center;
                max-width: 1000px;
                margin: 0 auto;
            }
            .search-button {
                margin: 20px 0;
            }
            h2, h4 {
                text-align: center;
                margin: 20px 0;
            }
            .example-box {
                background-color: #f8f9fa;
                border-radius: 8px;
                padding: 20px;
                margin: 20px auto;
                width: 80%;
                text-align: left;
            }
            .example-box p {
                font-weight: bold;
                margin-bottom: 10px;
                padding-left: 20px;
            }
            .example-box ul {
                margin: 0;
                padding-left: 40px;
            }
            .example-box li {
                word-wrap: break-word;
                margin: 10px 0;
                line-height: 1.5;
            }
            .query-label {
                display: block;
                text-align: left;
                margin-bottom: 10px;
                margin-left: 10%;
                font-weight: bold;
            }
            .status-box {
                background-color: #f8f9fa;
                border-radius: 8px;
                padding: 15px;
                margin: 20px auto;
                width: 80%;
                text-align: left;
            }
            .status-label {
                font-weight: bold;
                margin-right: 10px;
            }
            .status-ready {
                color: #198754;  /* Bootstrap success color */
                font-weight: bold;
            }
            .sequence-results {
                width: 80%;
                margin: 20px auto;
                text-align: left;
                font-family: monospace;
                white-space: pre-wrap;
                word-wrap: break-word;
                background-color: #f8f9fa;
                border-radius: 8px;
                padding: 20px;
                overflow-x: hidden;
            }
            .sequence-text {
                word-break: break-all;
                margin: 10px 0;
                line-height: 1.5;
            }
            .status-spinner {
                display: none;
                margin-left: 10px;
                vertical-align: middle;
            }
            .status-spinner.active {
                display: inline-block;
            }
        """)
    ),
    ui.div(
        {"class": "content-wrapper"},
        ui.h2("Advanced PDB Structure Search Tool"),
        ui.row(
            ui.column(12,
                ui.tags.label(
                    "Search Query", 
                    {"class": "query-label", "for": "query"}
                ),
                ui.input_text(
                    "query", 
                    "", 
                    value="Human insulin",
                    width="100%"
                ),
            )
        ),
        ui.row(
            ui.column(12,
                ui.div(
                    {"class": "example-box"},
                    ui.p("Example queries:"),
                    ui.tags.ul(
                        ui.tags.li("Human hemoglobin C resolution better than 2.5Å"),
                        ui.tags.li("Find structures containing sequence MNIFEMLRIDEGLRLKIYKDTEGYYTIGIGHLLTKSPSLNAAKSELDKAIGRNTNGVITKDEAEKLFNQDVDAAVRGILRNAKLKPVYDSLDAVRRAALINMVFQMGETGVAGFTNSLRMLQQKRWDEAAVNLAKSRWYNQTPNRAKRVITTFRTGTWDAYKNL"),
                        ui.tags.li("Sequence of PDB ID 8ET6")
                    )
                )
            )
        ),
        ui.row(
            ui.column(12,
                ui.div(
                    {"class": "search-button"},
                    ui.input_action_button("search", "Search", 
                                         class_="btn-primary btn-lg")  # 버튼 크기 증가
                )
            )
        ),
        ui.row(
            ui.column(12,
                ui.h4("Search Parameters:"),
                ui.div(
                    {"class": "status-box"},
                    ui.tags.span("Status: ", class_="status-label"),
                    ui.output_text("search_status", inline=True),
                    ui.tags.div(
                        {"class": "status-spinner"},
                        ui.tags.i({"class": "fas fa-spinner fa-spin"})
                    )
                )
            )
        ),
        ui.row(
            ui.column(12,
                ui.h4("Top 10 Results:"),
                output_widget("results_table"),
                ui.download_button("download", "Download Results", 
                                 class_="btn btn-info btn-lg")  # 다운로드 버튼 스타일 개선
            )
        ),
        ui.row(
            ui.column(12,
                ui.div(
                    {"class": "sequence-results", "id": "sequence-results"},
                    ui.h4("Sequences:"),
                    ui.output_text("sequence_output")
                )
            )
        ),
        ui.row(
            ui.column(12,
                ui.div(
                    {"class": "3d-iframe", "id": "3d-iframe"}, # css 미설정 
                    ui.h4("3D Rendering"),
                    ui.output_ui("output_iframe")
                )    
            )
        )
    )
)

def server(input, output, session):
    assistant = PDBSearchAssistant()
    results_store = reactive.Value({"type": None, "results": []})
    status_store = reactive.Value("Ready")
    
    @reactive.Effect
    @reactive.event(input.search)
    def _():
        # 검색 시작 시 상태 변경
        status_store.set("Searching...")
        
        # 프롬프트 처리
        query_results = assistant.process_query(input.query())
        results_store.set(query_results)
        
        if query_results["type"] == "sequence":
            if not query_results["results"]:
                status_store.set("No sequences found")
            else:
                status_store.set("Ready")  # 검색 완료 시 Ready로 변경
        else:
            df = pd.DataFrame(query_results["results"])
            status_store.set("Ready")  # 검색 완료 시 Ready로 변경
            
            @output
            @render_widget
            def results_table():
                return create_interactive_table(df)
    
    @output
    @render.text
    def search_status():
        return status_store.get()
    
    @output
    @render.download(filename="pdb_search_results.csv")
    def download():
        current_results = results_store.get()
        if current_results["type"] == "structure":
            df = pd.DataFrame(current_results["results"])
        else:
            df = pd.DataFrame(current_results["results"])
        return df.to_csv(index=False)

    @output
    @render.text
    def sequence_output():
        current_results = results_store.get()
        if current_results["type"] == "sequence":
            sequences = current_results["results"]
            if not sequences:
                return "No sequences found"
            
            output_text = []
            for seq in sequences:
                output_text.append(f"\nChain {seq['chain_id']} (Entity {seq['entity_id']}):")
                output_text.append(f"Description: {seq['description']}")
                output_text.append(f"Length: {seq['length']} residues")
                output_text.append("Sequence:")
                
                # 시퀀스를 60글자씩 나누어 줄바꿈
                sequence = seq['sequence']
                formatted_sequence = '\n'.join([sequence[i:i+60] for i in range(0, len(sequence), 60)])
                output_text.append(formatted_sequence)
                output_text.append("-" * 60)  # 구분선 길이도 조정
            
            return "\n".join(output_text)
        return ""
    
    @output
    @render.text
    def output_iframe():
        current_results = results_store.get()
        if current_results["type"] == "structure":
            pdb_id = current_results["results"][0]['PDB ID']
            # chain 가져오는 건 아직
            return render_html(pdb_id, "A")
        else:
            return ""

app = App(app_ui, server)

if __name__ == "__main__":
    import nest_asyncio
    nest_asyncio.apply()
    app.run(host="0.0.0.0", port=7862)