app.py

import numpy as np
import pandas as pd
import statsmodels.formula.api as smf
import statsmodels.api as sm
import plotly.graph_objects as go
from scipy.optimize import minimize
import plotly.express as px
from scipy.stats import t, f
import gradio as gr
import io
import zipfile
import tempfile
from datetime import datetime
import docx
from docx.shared import Inches, Pt
from docx.enum.text import WD_PARAGRAPH_ALIGNMENT
from matplotlib.colors import to_hex
import os

# --- Data definition in global scope ---
data_dict = {
    'Tratamiento': ['T1', 'T2', 'T3', 'T4', 'T5', 'T6', 'T7', 'T8', 'T9', 'T10', 'T11', 'T12', 'T13', 'T14', 'T15'] * 3,
    'Tiempo_fermentacion_h': [16] * 15 + [23] * 15 + [40] * 15,
    'pH': [6.02, 5.39, 6.27, 4.82, 6.25, 4.87, 4.76, 4.68, 4.64, 6.35, 4.67, 6.43, 4.58, 4.60, 6.96,
           5.17, 5.95, 6.90, 5.50, 5.08, 4.95, 5.41, 5.52, 4.98, 7.10, 5.36, 6.91, 5.21, 4.66, 7.10,
           5.42, 5.60, 7.36, 5.36, 4.66, 4.93, 5.18, 5.26, 4.92, 7.28, 5.26, 6.84, 5.19, 4.58, 7.07],
    'Abs_600nm': [1.576, 1.474, 1.293, 1.446, 1.537, 1.415, 1.481, 1.419, 1.321, 1.224, 1.459, 0.345, 1.279, 1.181, 0.662,
                  1.760, 1.690, 1.485, 1.658, 1.728, 1.594, 1.673, 1.607, 1.531, 1.424, 1.595, 0.344, 1.477, 1.257, 0.660,
                  1.932, 1.780, 1.689, 1.876, 1.885, 1.824, 1.913, 1.810, 1.852, 1.694, 1.831, 0.347, 1.752, 1.367, 0.656],
    'Glucosa_g_L': [5,10,0,5,10,5,10,5,10,0,5,0,5,5,0] * 3,
    'Proteina_Pescado_g_L': [1.4,1.4,3.2,3.2,3.2,3.2,3.2,5,5,5,5,0,5,5,0] * 3,
    'Sulfato_Manganeso_g_L': [0.75,0.5,0.75,0.5,0.75,0.5,0.75,0.5,0.25,0.75,0.5,0.25,0.5,0.25,0.5] * 3
}
data = pd.DataFrame(data_dict)
# --- End of data definition in global scope ---


# --- Clase RSM_BoxBehnken ---
class RSM_BoxBehnken:
    def __init__(self, data, x1_name, x2_name, x3_name, y_name, x1_levels, x2_levels, x3_levels):
        """
        Inicializa la clase con los datos del diseño Box-Behnken.
        """
        self.data = data.copy()
        self.model = None
        self.model_simplified = None
        self.optimized_results = None
        self.optimal_levels = None
        self.all_figures = []  # Lista para almacenar las figuras
        self.x1_name = x1_name
        self.x2_name = x2_name
        self.x3_name = x3_name
        self.y_name = y_name

        # Niveles originales de las variables
        self.x1_levels = x1_levels
        self.x2_levels = x2_levels
        self.x3_levels = x3_levels

    def get_levels(self, variable_name):
        """
        Obtiene los niveles para una variable específica.
        """
        if variable_name == self.x1_name:
            return self.x1_levels
        elif variable_name == self.x2_name:
            return self.x2_levels
        elif variable_name == self.x3_name:
            return self.x3_levels
        else:
            raise ValueError(f"Variable desconocida: {variable_name}")

    def fit_model(self):
        """
        Ajusta el modelo de segundo orden completo a los datos.
        """
        formula = f'{self.y_name} ~ {self.x1_name} + {self.x2_name} + {self.x3_name} + ' \
                  f'I({self.x1_name}**2) + I({self.x2_name}**2) + I({self.x3_name}**2) + ' \
                  f'{self.x1_name}:{self.x2_name} + {self.x1_name}:{self.x3_name} + {self.x2_name}:{self.x3_name}'
        self.model = smf.ols(formula, data=self.data).fit()
        print("Modelo Completo:")
        print(self.model.summary())
        return self.model, self.pareto_chart(self.model, "Pareto - Modelo Completo")

    def fit_simplified_model(self):
        """
        Ajusta el modelo de segundo orden a los datos, eliminando términos no significativos.
        """
        formula = f'{self.y_name} ~ {self.x1_name} + {self.x2_name} + ' \
                  f'I({self.x1_name}**2) + I({self.x2_name}**2) + I({self.x3_name}**2)' # Adjusted formula to include x3^2
        self.model_simplified = smf.ols(formula, data=self.data).fit()
        print("\nModelo Simplificado:")
        print(self.model_simplified.summary())
        return self.model_simplified, self.pareto_chart(self.model_simplified, "Pareto - Modelo Simplificado")

    def optimize(self, method='Nelder-Mead'):
        """
        Encuentra los niveles óptimos de los factores para maximizar la respuesta usando el modelo simplificado.
        """
        if self.model_simplified is None:
            print("Error: Ajusta el modelo simplificado primero.")
            return

        def objective_function(x):
            return -self.model_simplified.predict(pd.DataFrame({
                self.x1_name: [x[0]],
                self.x2_name: [x[1]],
                self.x3_name: [x[2]]
            })).values[0]

        bounds = [(-1, 1), (-1, 1), (-1, 1)]
        x0 = [0, 0, 0]

        self.optimized_results = minimize(objective_function, x0, method=method, bounds=bounds)
        self.optimal_levels = self.optimized_results.x

        # Convertir niveles óptimos de codificados a naturales
        optimal_levels_natural = [
            self.coded_to_natural(self.optimal_levels[0], self.x1_name),
            self.coded_to_natural(self.optimal_levels[1], self.x2_name),
            self.coded_to_natural(self.optimal_levels[2], self.x3_name)
        ]
        # Crear la tabla de optimización
        optimization_table = pd.DataFrame({
            'Variable': [self.x1_name, self.x2_name, self.x3_name],
            'Nivel Óptimo (Natural)': optimal_levels_natural,
            'Nivel Óptimo (Codificado)': self.optimal_levels
        })

        return optimization_table.round(3)  # Redondear a 3 decimales

    def plot_rsm_individual(self, fixed_variable, fixed_level):
        """
        Genera un gráfico de superficie de respuesta (RSM) individual para una configuración específica.
        """
        if self.model_simplified is None:
            print("Error: Ajusta el modelo simplificado primero.")
            return None

        # Determinar las variables que varían y sus niveles naturales
        varying_variables = [var for var in [self.x1_name, self.x2_name, self.x3_name] if var != fixed_variable]

        # Establecer los niveles naturales para las variables que varían
        x_natural_levels = self.get_levels(varying_variables[0])
        y_natural_levels = self.get_levels(varying_variables[1])

        # Crear una malla de puntos para las variables que varían (en unidades naturales)
        x_range_natural = np.linspace(x_natural_levels[0], x_natural_levels[-1], 100)
        y_range_natural = np.linspace(y_natural_levels[0], y_natural_levels[-1], 100)
        x_grid_natural, y_grid_natural = np.meshgrid(x_range_natural, y_range_natural)

        # Convertir la malla de variables naturales a codificadas
        x_grid_coded = self.natural_to_coded(x_grid_natural, varying_variables[0])
        y_grid_coded = self.natural_to_coded(y_grid_natural, varying_variables[1])

        # Crear un DataFrame para la predicción con variables codificadas
        prediction_data = pd.DataFrame({
            varying_variables[0]: x_grid_coded.flatten(),
            varying_variables[1]: y_grid_coded.flatten(),
        })
        prediction_data[fixed_variable] = self.natural_to_coded(fixed_level, fixed_variable)

        # Calcular los valores predichos
        z_pred = self.model_simplified.predict(prediction_data).values.reshape(x_grid_coded.shape)

        # Filtrar por el nivel de la variable fija (en codificado)
        fixed_level_coded = self.natural_to_coded(fixed_level, fixed_variable)
        subset_data = self.data[np.isclose(self.data[fixed_variable], fixed_level_coded)]

        # Filtrar por niveles válidos en las variables que varían
        valid_levels = [-1, 0, 1]
        experiments_data = subset_data[
            subset_data[varying_variables[0]].isin(valid_levels) &
            subset_data[varying_variables[1]].isin(valid_levels)
        ]

        # Convertir coordenadas de experimentos a naturales
        experiments_x_natural = experiments_data[varying_variables[0]].apply(lambda x: self.coded_to_natural(x, varying_variables[0]))
        experiments_y_natural = experiments_data[varying_variables[1]].apply(lambda x: self.coded_to_natural(x, varying_variables[1]))

        # Crear el gráfico de superficie con variables naturales en los ejes y transparencia
        fig = go.Figure(data=[go.Surface(z=z_pred, x=x_grid_natural, y=y_grid_natural, colorscale='Viridis', opacity=0.7, showscale=True)])

        # --- Añadir cuadrícula a la superficie ---
        # Líneas en la dirección x
        for i in range(x_grid_natural.shape[0]):
            fig.add_trace(go.Scatter3d(
                x=x_grid_natural[i, :],
                y=y_grid_natural[i, :],
                z=z_pred[i, :],
                mode='lines',
                line=dict(color='gray', width=2),
                showlegend=False,
                hoverinfo='skip'
            ))
        # Líneas en la dirección y
        for j in range(x_grid_natural.shape[1]):
            fig.add_trace(go.Scatter3d(
                x=x_grid_natural[:, j],
                y=y_grid_natural[:, j],
                z=z_pred[:, j],
                mode='lines',
                line=dict(color='gray', width=2),
                showlegend=False,
                hoverinfo='skip'
            ))

        # --- Fin de la adición de la cuadrícula ---

        # Añadir los puntos de los experimentos en la superficie de respuesta con diferentes colores y etiquetas
        colors = px.colors.qualitative.Safe
        point_labels = [f"{row[self.y_name]:.3f}" for _, row in experiments_data.iterrows()]

        fig.add_trace(go.Scatter3d(
            x=experiments_x_natural,
            y=experiments_y_natural,
            z=experiments_data[self.y_name].round(3),
            mode='markers+text',
            marker=dict(size=4, color=colors[:len(experiments_x_natural)]),
            text=point_labels,
            textposition='top center',
            name='Experimentos'
        ))

        # Añadir etiquetas y título con variables naturales
        fig.update_layout(
            scene=dict(
                xaxis_title=f"{varying_variables[0]} ({self.get_units(varying_variables[0])})",
                yaxis_title=f"{varying_variables[1]} ({self.get_units(varying_variables[1])})",
                zaxis_title=self.y_name,
            ),
            title=f"{self.y_name} vs {varying_variables[0]} y {varying_variables[1]}<br><sup>{fixed_variable} fijo en {fixed_level:.3f} ({self.get_units(fixed_variable)}) (Modelo Simplificado)</sup>",
            height=800,
            width=1000,
            showlegend=True
        )
        return fig

    def get_units(self, variable_name):
        """
        Define las unidades de las variables para etiquetas.
        Puedes personalizar este método según tus necesidades.
        """
        units = {
            'Glucosa_g_L': 'g/L',
            'Proteina_Pescado_g_L': 'g/L',
            'Sulfato_Manganeso_g_L': 'g/L',
            'Abs_600nm': '' # No units for Absorbance
        }
        return units.get(variable_name, '')

    def generate_all_plots(self):
        """
        Genera todas las gráficas de RSM, variando la variable fija y sus niveles usando el modelo simplificado.
        Almacena las figuras en self.all_figures.
        """
        if self.model_simplified is None:
            print("Error: Ajusta el modelo simplificado primero.")
            return

        self.all_figures = []  # Resetear la lista de figuras

        # Niveles naturales para graficar - Using levels from the data context, not Box-Behnken design levels.
        levels_to_plot_natural = {
            self.x1_name: sorted(list(set(self.data[self.x1_name]))), # Using unique values from data
            self.x2_name: sorted(list(set(self.data[self.x2_name]))), # Using unique values from data
            self.x3_name: sorted(list(set(self.data[self.x3_name])))  # Using unique values from data
        }

        # Generar y almacenar gráficos individuales
        for fixed_variable in [self.x1_name, self.x2_name, self.x3_name]:
            for level in levels_to_plot_natural[fixed_variable]:
                fig = self.plot_rsm_individual(fixed_variable, level)
                if fig is not None:
                    self.all_figures.append(fig)

    def coded_to_natural(self, coded_value, variable_name):
        """Convierte un valor codificado a su valor natural."""
        levels = self.get_levels(variable_name)
        return levels[0] + (coded_value + 1) * (levels[-1] - levels[0]) / 2

    def natural_to_coded(self, natural_value, variable_name):
        """Convierte un valor natural a su valor codificado."""
        levels = self.get_levels(variable_name)
        return -1 + 2 * (natural_value - levels[0]) / (levels[-1] - levels[0])

    def pareto_chart(self, model, title):
        """
        Genera un diagrama de Pareto para los efectos estandarizados de un modelo,
        incluyendo la línea de significancia.
        """
        # Calcular los efectos estandarizados
        tvalues = model.tvalues[1:]  # Excluir la Intercept
        abs_tvalues = np.abs(tvalues)
        sorted_idx = np.argsort(abs_tvalues)[::-1]
        sorted_tvalues = abs_tvalues[sorted_idx]
        sorted_names = tvalues.index[sorted_idx]

        # Calcular el valor crítico de t para la línea de significancia
        alpha = 0.05  # Nivel de significancia
        dof = model.df_resid  # Grados de libertad residuales
        t_critical = t.ppf(1 - alpha / 2, dof)

        # Crear el diagrama de Pareto
        fig = px.bar(
            x=sorted_tvalues.round(3),
            y=sorted_names,
            orientation='h',
            labels={'x': 'Efecto Estandarizado', 'y': 'Término'},
            title=title
        )
        fig.update_yaxes(autorange="reversed")

        # Agregar la línea de significancia
        fig.add_vline(x=t_critical, line_dash="dot",
                      annotation_text=f"t crítico = {t_critical:.3f}",
                      annotation_position="bottom right")

        return fig

    def get_simplified_equation(self):
        """
        Retorna la ecuación del modelo simplificado como una cadena de texto.
        """
        if self.model_simplified is None:
            print("Error: Ajusta el modelo simplificado primero.")
            return None

        coefficients = self.model_simplified.params
        equation = f"{self.y_name} = {coefficients['Intercept']:.3f}"

        for term, coef in coefficients.items():
            if term != 'Intercept':
                if term == f'{self.x1_name}':
                    equation += f" + {coef:.3f}*{self.x1_name}"
                elif term == f'{self.x2_name}':
                    equation += f" + {coef:.3f}*{self.x2_name}"
                elif term == f'{self.x3_name}':
                    equation += f" + {coef:.3f}*{self.x3_name}"
                elif term == f'I({self.x1_name} ** 2)':
                    equation += f" + {coef:.3f}*{self.x1_name}^2"
                elif term == f'I({self.x2_name} ** 2)':
                    equation += f" + {coef:.3f}*{self.x2_name}^2"
                elif term == f'I({self.x3_name} ** 2)':
                    equation += f" + {coef:.3f}*{self.x3_name}^2"

        return equation

    def generate_prediction_table(self):
        """
        Genera una tabla con los valores actuales, predichos y residuales.
        """
        if self.model_simplified is None:
            print("Error: Ajusta el modelo simplificado primero.")
            return None

        self.data['Predicho'] = self.model_simplified.predict(self.data)
        self.data['Residual'] = self.data[self.y_name] - self.data['Predicho']

        return self.data[[self.y_name, 'Predicho', 'Residual']].round(3)

    def calculate_contribution_percentage(self):
        """
        Calcula el porcentaje de contribución de cada factor a la variabilidad de la respuesta (AIA).
        """
        if self.model_simplified is None:
            print("Error: Ajusta el modelo simplificado primero.")
            return None

        # ANOVA del modelo simplificado
        anova_table = sm.stats.anova_lm(self.model_simplified, typ=2)

        # Suma de cuadrados total
        ss_total = anova_table['sum_sq'].sum()

        # Crear tabla de contribución
        contribution_table = pd.DataFrame({
            'Factor': [],
            'Suma de Cuadrados': [],
            '% Contribución': []
        })

        # Calcular porcentaje de contribución para cada factor
        for index, row in anova_table.iterrows():
            if index != 'Residual':
                factor_name = index
                if factor_name == f'I({self.x1_name} ** 2)':
                    factor_name = f'{self.x1_name}^2'
                elif factor_name == f'I({self.x2_name} ** 2)':
                    factor_name = f'{self.x2_name}^2'
                elif factor_name == f'I({self.x3_name} ** 2)':
                    factor_name = f'{self.x3_name}^2'

                ss_factor = row['sum_sq']
                contribution_percentage = (ss_factor / ss_total) * 100

                contribution_table = pd.concat([contribution_table, pd.DataFrame({
                    'Factor': [factor_name],
                    'Suma de Cuadrados': [ss_factor],
                    '% Contribución': [contribution_percentage]
                })], ignore_index=True)

        return contribution_table.round(3)

    def calculate_detailed_anova(self):
        """
        Calcula la tabla ANOVA detallada con la descomposición del error residual.
        """
        if self.model_simplified is None:
            print("Error: Ajusta el modelo simplificado primero.")
            return None

        # --- ANOVA detallada ---
        # 1. Ajustar un modelo solo con los términos de primer orden y cuadráticos
        formula_reduced = f'{self.y_name} ~ {self.x1_name} + {self.x2_name} + {self.x3_name} + ' \
                          f'I({self.x1_name}**2) + I({self.x2_name}**2) + I({self.x3_name}**2)'
        model_reduced = smf.ols(formula_reduced, data=self.data).fit()

        # 2. ANOVA del modelo reducido (para obtener la suma de cuadrados de la regresión)
        anova_reduced = sm.stats.anova_lm(model_reduced, typ=2)

        # 3. Suma de cuadrados total
        ss_total = np.sum((self.data[self.y_name] - self.data[self.y_name].mean())**2)

        # 4. Grados de libertad totales
        df_total = len(self.data) - 1

        # 5. Suma de cuadrados de la regresión
        ss_regression = anova_reduced['sum_sq'][:-1].sum()  # Sumar todo excepto 'Residual'

        # 6. Grados de libertad de la regresión
        df_regression = len(anova_reduced) - 1

        # 7. Suma de cuadrados del error residual
        ss_residual = self.model_simplified.ssr
        df_residual = self.model_simplified.df_resid

        # 8. Suma de cuadrados del error puro (se calcula a partir de las réplicas)
        replicas = self.data[self.data.duplicated(subset=[self.x1_name, self.x2_name, self.x3_name], keep=False)]
        if not replicas.empty:
            ss_pure_error = replicas.groupby([self.x1_name, self.x2_name, self.x3_name])[self.y_name].var().sum() * replicas.groupby([self.x1_name, self.x2_name, self.x3_name]).ngroups
            df_pure_error = len(replicas) - replicas.groupby([self.x1_name, self.x2_name, self.x3_name]).ngroups
        else:
            ss_pure_error = np.nan
            df_pure_error = np.nan

        # 9. Suma de cuadrados de la falta de ajuste
        ss_lack_of_fit = ss_residual - ss_pure_error if not np.isnan(ss_pure_error) else np.nan
        df_lack_of_fit = df_residual - df_pure_error if not np.isnan(df_pure_error) else np.nan

        # 10. Cuadrados medios
        ms_regression = ss_regression / df_regression
        ms_residual = ss_residual / df_residual
        ms_lack_of_fit = np.nan # Initialize ms_lack_of_fit to nan
        if not np.isnan(df_lack_of_fit) and df_lack_of_fit != 0: # Check df_lack_of_fit is valid
            ms_lack_of_fit = ss_lack_of_fit / df_lack_of_fit
        ms_pure_error = ss_pure_error / df_pure_error if not np.isnan(df_pure_error) else np.nan

        # 11. Estadístico F y valor p para la falta de ajuste
        f_lack_of_fit = ms_lack_of_fit / ms_pure_error if not np.isnan(ms_lack_of_fit) and not np.isnan(ms_pure_error) and ms_pure_error != 0 else np.nan # Added nan checks and zero division check
        p_lack_of_fit = 1 - f.cdf(f_lack_of_fit, df_lack_of_fit, df_pure_error) if not np.isnan(f_lack_of_fit) and not np.isnan(df_lack_of_fit) and not np.isnan(df_pure_error) else np.nan # Added nan checks


        # 12. Crear la tabla ANOVA detallada
        detailed_anova_table = pd.DataFrame({
            'Fuente de Variación': ['Regresión', 'Residual', 'Falta de Ajuste', 'Error Puro', 'Total'],
            'Suma de Cuadrados': [ss_regression, ss_residual, ss_lack_of_fit, ss_pure_error, ss_total],
            'Grados de Libertad': [df_regression, df_residual, df_lack_of_fit, df_pure_error, df_total],
            'Cuadrado Medio': [ms_regression, ms_residual, ms_lack_of_fit, ms_pure_error, np.nan],
            'F': [np.nan, np.nan, f_lack_of_fit, np.nan, np.nan],
            'Valor p': [np.nan, np.nan, p_lack_of_fit, np.nan, np.nan]
        })

        # Calcular la suma de cuadrados y grados de libertad para la curvatura
        ss_curvature = anova_reduced['sum_sq'][f'I({self.x1_name} ** 2)'] + anova_reduced['sum_sq'][f'I({self.x2_name} ** 2)'] + anova_reduced['sum_sq'][f'I({self.x3_name} ** 2)']
        df_curvature = 3

        # Añadir la fila de curvatura a la tabla ANOVA
        detailed_anova_table.loc[len(detailed_anova_table)] = ['Curvatura', ss_curvature, df_curvature, ss_curvature / df_curvature, np.nan, np.nan]

        # Reorganizar las filas para que la curvatura aparezca después de la regresión
        detailed_anova_table = detailed_anova_table.reindex([0, 5, 1, 2, 3, 4])

        # Resetear el índice para que sea consecutivo
        detailed_anova_table = detailed_anova_table.reset_index(drop=True)

        return detailed_anova_table.round(3)

    def get_all_tables(self):
        """
        Obtiene todas las tablas generadas para ser exportadas a Excel.
        """
        prediction_table = self.generate_prediction_table()
        contribution_table = self.calculate_contribution_percentage()
        detailed_anova_table = self.calculate_detailed_anova()

        return {
            'Predicciones': prediction_table,
            '% Contribución': contribution_table,
            'ANOVA Detallada': detailed_anova_table
        }

    def save_figures_to_zip(self):
        """
        Guarda todas las figuras almacenadas en self.all_figures a un archivo ZIP en memoria.
        """
        if not self.all_figures:
            return None

        zip_buffer = io.BytesIO()
        with zipfile.ZipFile(zip_buffer, 'w') as zip_file:
            for idx, fig in enumerate(self.all_figures, start=1):
                img_bytes = fig.to_image(format="png")
                zip_file.writestr(f'Grafico_{idx}.png', img_bytes)
        zip_buffer.seek(0)

        # Guardar en un archivo temporal
        with tempfile.NamedTemporaryFile(delete=False, suffix=".zip") as temp_file:
            temp_file.write(zip_buffer.read())
            temp_path = temp_file.name

        return temp_path

    def save_fig_to_bytes(self, fig):
        """
        Convierte una figura Plotly a bytes en formato PNG.
        """
        return fig.to_image(format="png")

    def save_all_figures_png(self):
        """
        Guarda todas las figuras en archivos PNG temporales y retorna las rutas.
        """
        png_paths = []
        for idx, fig in enumerate(self.all_figures, start=1):
            img_bytes = fig.to_image(format="png")
            with tempfile.NamedTemporaryFile(delete=False, suffix=".png") as temp_file:
                temp_file.write(img_bytes)
                temp_path = temp_file.name
                png_paths.append(temp_path)
        return png_paths

    def save_tables_to_excel(self):
        """
        Guarda todas las tablas en un archivo Excel con múltiples hojas y retorna la ruta del archivo.
        """
        tables = self.get_all_tables()
        excel_buffer = io.BytesIO()
        with pd.ExcelWriter(excel_buffer, engine='xlsxwriter') as writer:
            for sheet_name, table in tables.items():
                table.to_excel(writer, sheet_name=sheet_name, index=False)
        excel_buffer.seek(0)
        excel_bytes = excel_buffer.read()

        # Guardar en un archivo temporal
        with tempfile.NamedTemporaryFile(delete=False, suffix=".xlsx") as temp_file:
            temp_file.write(excel_bytes)
            temp_path = temp_file.name

        return temp_path

    def export_tables_to_word(self, tables_dict):
        """
        Exporta las tablas proporcionadas a un documento de Word.
        """
        if not tables_dict:
            return None

        doc = docx.Document()

        # Configurar estilo de fuente
        style = doc.styles['Normal']
        font = style.font
        font.name = 'Times New Roman'
        font.size = Pt(12)

        # Título del informe
        titulo = doc.add_heading('Informe de Optimización de Producción de Absorbancia', 0) # Changed Title
        titulo.alignment = WD_PARAGRAPH_ALIGNMENT.CENTER

        doc.add_paragraph(f"Fecha: {datetime.now().strftime('%d/%m/%Y %H:%M')}").alignment = WD_PARAGRAPH_ALIGNMENT.CENTER

        doc.add_paragraph('\n')  # Espacio

        for sheet_name, table in tables_dict.items():
            # Añadir título de la tabla
            doc.add_heading(sheet_name, level=1)

            if table.empty:
                doc.add_paragraph("No hay datos disponibles para esta tabla.")
                continue

            # Añadir tabla al documento
            table_doc = doc.add_table(rows=1, cols=len(table.columns))
            table_doc.style = 'Light List Accent 1'

            # Añadir encabezados
            hdr_cells = table_doc.rows[0].cells
            for idx, col_name in enumerate(table.columns):
                hdr_cells[idx].text = col_name

            # Añadir filas de datos
            for _, row in table.iterrows():
                row_cells = table_doc.add_row().cells
                for idx, item in enumerate(row):
                    row_cells[idx].text = str(item)

            doc.add_paragraph('\n')  # Espacio entre tablas

        # Guardar el documento en un archivo temporal
        with tempfile.NamedTemporaryFile(delete=False, suffix=".docx") as tmp:
            doc.save(tmp.name)
            tmp_path = tmp.name

        return tmp_path

# --- Funciones para la Interfaz de Gradio ---

def load_data(data_str): # Modified load_data to only take data_str
    """
    Carga los datos del diseño Box-Behnken desde cajas de texto y crea la instancia de RSM_BoxBehnken.
    """
    try:
        # Use the global data DataFrame
        global rsm, data

        x1_name = "Glucosa_g_L"
        x2_name = "Proteina_Pescado_g_L"
        x3_name = "Sulfato_Manganeso_g_L"
        y_name = "Abs_600nm"
        x1_levels = sorted(list(set(data[x1_name]))) # Levels from data
        x2_levels = sorted(list(set(data[x2_name]))) # Levels from data
        x3_levels = sorted(list(set(data[x3_name]))) # Levels from data


        # Crear la instancia de RSM_BoxBehnken
        rsm = RSM_BoxBehnken(data, x1_name, x2_name, x3_name, y_name, x1_levels, x2_levels, x3_levels)

        return data.round(3), gr.update(visible=True) # Removed other outputs, only return data_output and analysis_row

    except Exception as e:
        # Mostrar mensaje de error
        error_message = f"Error al cargar los datos: {str(e)}"
        print(error_message)
        return None, gr.update(visible=False) # Removed other outputs, only return data_output and analysis_row


def fit_and_optimize_model():
    if 'rsm' not in globals():
        return [None]*11  # Ajustar el número de outputs

    # Ajustar modelos y optimizar
    model_completo, pareto_completo = rsm.fit_model()
    model_simplificado, pareto_simplificado = rsm.fit_simplified_model()
    optimization_table = rsm.optimize()
    equation = rsm.get_simplified_equation()
    prediction_table = rsm.generate_prediction_table()
    contribution_table = rsm.calculate_contribution_percentage()
    anova_table = rsm.calculate_detailed_anova()

    # Generar todas las figuras and store them in rsm.all_figures
    rsm.generate_all_plots()

    # Formatear la ecuación para que se vea mejor en Markdown
    equation_formatted = equation.replace(" + ", "<br>+ ").replace(" ** ", "^").replace("*", " × ")
    equation_formatted = f"### Ecuación del Modelo Simplificado:<br>{equation_formatted}"

    # Guardar las tablas en Excel temporal
    excel_path = rsm.save_tables_to_excel()
    zip_path = rsm.save_figures_to_zip()

    return (
        model_completo.summary().as_html(),
        pareto_completo,
        model_simplificado.summary().as_html(),
        pareto_simplificado,
        equation_formatted,
        optimization_table,
        prediction_table,
        contribution_table,
        anova_table,
        zip_path,
        excel_path
    )

def show_plot(current_index, all_figures):
    if not all_figures:
        return None, "No hay gráficos disponibles.", current_index
    selected_fig = all_figures[current_index]
    plot_info_text = f"Gráfico {current_index + 1} de {len(all_figures)}"
    return selected_fig, plot_info_text, current_index

def navigate_plot(direction, current_index, all_figures):
    """
    Navega entre los gráficos.
    """
    if not all_figures:
        return None, "No hay gráficos disponibles.", current_index

    if direction == 'left':
        new_index = (current_index - 1) % len(all_figures)
    elif direction == 'right':
        new_index = (current_index + 1) % len(all_figures)
    else:
        new_index = current_index

    selected_fig = all_figures[new_index]
    plot_info_text = f"Gráfico {new_index + 1} de {len(all_figures)}"

    return selected_fig, plot_info_text, current_index

def download_current_plot(all_figures, current_index):
    """
    Descarga la figura actual como PNG.
    """
    if not all_figures:
        return None
    fig = all_figures[current_index]
    img_bytes = rsm.save_fig_to_bytes(fig)
    filename = f"Grafico_RSM_{current_index + 1}.png"

    # Crear un archivo temporal
    with tempfile.NamedTemporaryFile(delete=False, suffix=".png") as temp_file:
        temp_file.write(img_bytes)
        temp_path = temp_file.name

    return temp_path  # Retornar solo la ruta

def download_all_plots_zip():
    """
    Descarga todas las figuras en un archivo ZIP.
    """
    if 'rsm' not in globals():
        return None
    zip_path = rsm.save_figures_to_zip()
    if zip_path:
        filename = f"Graficos_RSM_{datetime.now().strftime('%Y%m%d_%H%M%S')}.zip"
        # Gradio no permite renombrar directamente, por lo que retornamos la ruta del archivo
        return zip_path
    return None

def download_all_tables_excel():
    """
    Descarga todas las tablas en un archivo Excel con múltiples hojas.
    """
    if 'rsm' not in globals():
        return None
    excel_path = rsm.save_tables_to_excel()
    if excel_path:
        filename = f"Tablas_RSM_{datetime.now().strftime('%Y%m%d_%H%M%S')}.xlsx"
        # Gradio no permite renombrar directamente, por lo que retornamos la ruta del archivo
        return excel_path
    return None

def exportar_word(rsm_instance, tables_dict):
    """
    Función para exportar las tablas a un documento de Word.
    """
    word_path = rsm_instance.export_tables_to_word(tables_dict)
    if word_path and os.path.exists(word_path):
        return word_path
    return None

# --- Crear la interfaz de Gradio ---

def create_gradio_interface():
    with gr.Blocks() as demo:
        gr.Markdown("# Optimización de la Absorbancia usando RSM") # Changed Title

        with gr.Row():
            with gr.Column():
                gr.Markdown("## Configuración del Análisis") # Changed Section Title
                # Removed input boxes for variable names and levels
                data_input = gr.Textbox(label="Datos del Experimento (formato CSV - Ignored, Data is Hardcoded)", lines=5, interactive=False, value="Data is pre-loaded, ignore input.") # Adjusted Textbox
                load_button = gr.Button("Cargar Datos") # Keep load button for triggering data load but input is ignored

            with gr.Column():
                gr.Markdown("## Datos Cargados")
                data_output = gr.Dataframe(label="Tabla de Datos", interactive=False)

        # Sección de análisis visible solo después de cargar los datos
        with gr.Row(visible=False) as analysis_row:
            with gr.Column():
                fit_button = gr.Button("Ajustar Modelo y Optimizar")
                gr.Markdown("**Modelo Completo**")
                model_completo_output = gr.HTML()
                pareto_completo_output = gr.Plot()
                gr.Markdown("**Modelo Simplificado**")
                model_simplificado_output = gr.HTML()
                pareto_simplificado_output = gr.Plot()
                gr.Markdown("**Ecuación del Modelo Simplificado**")
                equation_output = gr.HTML()
                optimization_table_output = gr.Dataframe(label="Tabla de Optimización", interactive=False)
                prediction_table_output = gr.Dataframe(label="Tabla de Predicciones", interactive=False)
                contribution_table_output = gr.Dataframe(label="Tabla de % de Contribución", interactive=False)
                anova_table_output = gr.Dataframe(label="Tabla ANOVA Detallada", interactive=False)
                gr.Markdown("## Descargar Todas las Tablas")
                download_excel_button = gr.DownloadButton("Descargar Tablas en Excel")
                download_word_button = gr.DownloadButton("Descargar Tablas en Word")

            with gr.Column():
                gr.Markdown("## Generar Gráficos de Superficie de Respuesta")
                fixed_variable_input = gr.Dropdown(label="Variable Fija", choices=["Glucosa_g_L", "Proteina_Pescado_g_L", "Sulfato_Manganeso_g_L"], value="Glucosa_g_L") # Updated choices
                fixed_level_input = gr.Slider(label="Nivel de Variable Fija (Natural Units)", minimum=min(data['Glucosa_g_L']), maximum=max(data['Glucosa_g_L']), step=0.1, value=5.0) # Updated Slider - Using data min/max
                plot_button = gr.Button("Generar Gráficos")
                with gr.Row():
                    left_button = gr.Button("<")
                    right_button = gr.Button(">")
                rsm_plot_output = gr.Plot()
                plot_info = gr.Textbox(label="Información del Gráfico", value="Gráfico 1 de 9", interactive=False)
                with gr.Row():
                    download_plot_button = gr.DownloadButton("Descargar Gráfico Actual (PNG)")
                    download_all_plots_button = gr.DownloadButton("Descargar Todos los Gráficos (ZIP)")
                current_index_state = gr.State(0)  # Estado para el índice actual
                all_figures_state = gr.State([])  # Estado para todas las figuras

        # Cargar datos - Modified load_button click to only take data_input (which is ignored)
        load_button.click(
            load_data,
            inputs=[data_input], # Only data_input is now input
            outputs=[data_output, analysis_row] # Removed other outputs, only return data_output and analysis_row
        )

        # Ajustar modelo y optimizar
        fit_button.click(
            fit_and_optimize_model,
            inputs=[],
            outputs=[ # Corrected outputs to return calculated values not output components
                model_completo_output, # This should be removed, returning summary HTML string instead
                pareto_completo_output, # This should be removed, returning Plotly Figure instead
                model_simplificado_output, # This should be removed, returning summary HTML string instead
                pareto_simplificado_output, # This should be removed, returning Plotly Figure instead
                equation_output, # This should be removed, returning formatted equation string instead
                optimization_table_output, # This should be removed, returning DataFrame instead
                prediction_table_output, # This should be removed, returning DataFrame instead
                contribution_table_output, # This should be removed, returning DataFrame instead
                anova_table_output, # This should be removed, returning DataFrame instead
                download_all_plots_button, # Correct - returning file path for download button
                download_excel_button # Correct - returning file path for download button
            ]
        )

        # Generar y mostrar los gráficos
        plot_button.click(
            lambda fixed_var, fixed_lvl: (
                rsm.plot_rsm_individual(fixed_var, fixed_lvl),
                f"Gráfico 1 de {len(rsm.all_figures)}" if rsm.all_figures else "No hay gráficos disponibles.",
                0,
                rsm.all_figures  # Actualizar el estado de todas las figuras
            ),
            inputs=[fixed_variable_input, fixed_level_input],
            outputs=[rsm_plot_output, plot_info, current_index_state, all_figures_state]
        )

        # Navegación de gráficos
        left_button.click(
            lambda current_index, all_figures: navigate_plot('left', current_index, all_figures),
            inputs=[current_index_state, all_figures_state],
            outputs=[rsm_plot_output, plot_info, current_index_state]
        )
        right_button.click(
            lambda current_index, all_figures: navigate_plot('right', current_index, all_figures),
            inputs=[current_index_state, all_figures_state],
            outputs=[rsm_plot_output, plot_info, current_index_state]
        )

        # Descargar gráfico actual
        download_plot_button.click(
            download_current_plot,
            inputs=[all_figures_state, current_index_state],
            outputs=download_plot_button
        )

        # Descargar todos los gráficos en ZIP
        download_all_plots_button.click(
            download_all_plots_zip,
            inputs=[],
            outputs=download_all_plots_button
        )

        # Descargar todas las tablas en Excel y Word
        download_excel_button.click(
            fn=lambda: download_all_tables_excel(),
            inputs=[],
            outputs=download_excel_button
        )

        download_word_button.click(
            fn=lambda: exportar_word(rsm, rsm.get_all_tables()),
            inputs=[],
            outputs=download_word_button
        )

        # Ejemplo de uso
        gr.Markdown("## Instrucciones:") # Shortened Instructions
        gr.Markdown("""
        1. Click 'Cargar Datos' para usar los datos precargados.
        2. Click 'Ajustar Modelo y Optimizar'.
        3. Select 'Variable Fija' and 'Nivel de Variable Fija'.
        4. Click 'Generar Gráficos'.
        5. Navigate plots with '<' and '>'.
        6. Download plots and tables as needed.
        """)

    return demo

# --- Función Principal ---

def main():
    interface = create_gradio_interface()
    interface.launch(share=True)

if __name__ == "__main__":
    main()