Datasets:

---

siddharthtumre

/

Revised-JNLPBA

like 0

[ "We", "identified", "a", "stretch", "of", "23", "amino", "acids", ",", "rich", "in", "serine", "and", "threonine", "residues", ",", "which", "was", "responsible", "for", "most", "of", "the", "repression", "activity", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "show", "that", "repression", "is", "strongly", "dependent", "on", "the", "type", "of", "enhancer", "present", "in", "the", "reporter", "plasmid", "as", "well", "as", "on", "the", "cell", "line", "tested", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "possibility", "that", "Oct-2a", "can", "act", "as", "an", "activator", "and/or", "a", "repressor", "may", "have", "important", "consequences", "for", "the", "function", "of", "Oct-2a", "in", "B", "cell", "differentiation", "and", "other", "developmental", "processes", "." ]

[ 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 7, 8, 0, 0, 0, 0, 0, 0 ]

[ "Opposing", "effects", "of", "glucocorticoids", "on", "the", "rate", "of", "apoptosis", "in", "neutrophilic", "and", "eosinophilic", "granulocytes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "Eosinophils", "and", "neutrophils", "are", "closely", "related", ",", "terminally", "differentiated", "cells", "that", "in", "vitro", "undergo", "constitutive", "cell", "death", "by", "apoptosis", "." ]

[ 7, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "onset", "of", "apoptosis", "in", "both", "cell", "types", "can", "be", "delayed", "by", "hemopoietins", "and", "inflammatory", "mediators", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]

[ "Although", "there", "have", "been", "a", "number", "of", "reports", "demonstrating", "that", "glucocorticoids", "(", "in", "particular", "dexamethasone", ")", "antagonize", "the", "eosinophil", "life-prolonging", "effects", "of", "hemopoietins", ",", "direct", "effects", "of", "dexamethasone", "on", "eosinophil", "apoptosis", "have", "not", "been", "documented", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0 ]

[ "In", "this", "study", "we", "examined", "the", "direct", "effects", "of", "glucocorticoids", "on", "eosinophil", "and", "neutrophil", "apoptosis", "in", "light", "of", "their", "common", "therapeutic", "use", "as", "anti-inflammatory", "and", "anti-allergic/hypereosinophilic", "agents", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "found", "that", "treatment", "with", "dexamethasone", "induced", "eosinophil", "apoptosis", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 7, 0, 0 ]

[ "In", "contrast", ",", "dexamethasone", "was", "a", "potent", "inhibitor", "of", "neutrophil", "apoptosis", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0 ]

[ "The", "effect", "of", "dexamethasone", "on", "both", "cell", "types", "was", "mediated", "through", "the", "glucocorticoid", "receptor", ",", "i.e.", ",", "it", "was", "abolished", "by", "the", "glucocorticoid", "receptor", "antagonist", "RU38486", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0 ]

[ "This", "is", "the", "first", "description", "of", "an", "agent", "that", "promotes", "eosinophil", "apoptosis", "while", "inhibiting", "neutrophil", "apoptosis", ",", "and", "thus", "presents", "a", "novel", "approach", "to", "the", "study", "of", "control", "of", "apoptosis", "in", "these", "closely", "related", "cell", "types", "as", "well", "as", "increases", "our", "understanding", "of", "the", "clinical", "action", "of", "glucocorticoids", "in", "inflammation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Permanent", "occupancy", "of", "the", "human", "immunodeficiency", "virus", "type", "1", "enhancer", "by", "NF-kappa", "B", "is", "needed", "for", "persistent", "viral", "replication", "in", "monocytes", "." ]

[ 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 7, 0 ]

[ "This", "work", "aimed", "to", "ascertain", "the", "role", "of", "kappaB-responsive", "elements", "of", "the", "human", "immunodeficiency", "virus", "type", "1", "(", "HIV-1", ")", "enhancer", "not", "only", "in", "early", "initiation", "but", "also", "in", "long-term", "maintenance", "of", "proviral", "transcription", "in", "cells", "of", "the", "monocytic", "lineage", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "For", "this", "purpose", ",", "we", "used", "three", "main", "approaches", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "first", "was", "to", "abruptly", "terminate", "tumor", "necrosis", "factor-induced", "NF-kappaB", "binding", "to", "the", "enhancer", "sequences", "in", "U1", "monocytic", "cells", ",", "using", "a", "short", "pulse", "of", "exogenous", "tumor", "necrosis", "factor", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "This", "resulted", "in", "concomitant", "decrease", "in", "nuclear", "NF-kappaB", "DNA-binding", "activity", "and", "endogenous", "long", "terminal", "repeat", "transcriptional", "activity", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "second", "was", "to", "suppress", "the", "permanent", "NF-kappaB", "translocation", "induced", "by", "HIV-1", "replication", "itself", "in", "chronically", "infected", "U937", "cells", ",", "using", "a", "specific", "proteasome", "inhibitor", "(", "Z-LLL-H", ")", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "As", "early", "as", "2", "h", "after", "addition", "of", "the", "inhibitor", "to", "the", "culture", "medium", ",", "there", "was", "an", "inhibition", "of", "both", "constitutive", "activation", "of", "NF-kappaB", "and", "HIV-1", "genome", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0 ]

[ "The", "third", "approach", "was", "to", "monitor", "the", "replication", "competence", "in", "U937", "cells", "of", "an", "infectious", "HIV-1", "provirus", "carrying", "point", "mutations", "in", "the", "kappaB-responsive", "elements", "of", "both", "long", "terminal", "repeats", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0 ]

[ "Compared", "with", "its", "wild-type", "counterpart", ",", "this", "mutated", "provirus", "showed", "a", "profoundly", "decreased", ",", "Z-LLL-H-insensitive", "transcriptional", "and", "replicative", "activity", "in", "U937", "monocytes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0 ]

[ "Together", ",", "our", "results", "indicate", "that", "occupancy", "of", "the", "viral", "enhancer", "by", "NF-kappaB", "(", "p50/p65", ")", "heterodimers", "is", "required", "for", "ongoing", "transcription", "of", "integrated", "HIV", "provirus", "in", "monocytes", ",", "even", "in", "cells", "chronically", "infected", "and", "permanently", "producing", "functional", "HIV", "Tat", "protein", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "Thus", ",", "the", "ability", "of", "HIV-1", "replication", "to", "activate", "NF-kappaB", "is", "crucial", "to", "the", "intense", "self-perpetuated", "viral", "transcription", "observed", "in", "cells", "of", "the", "monocytic", "lineage", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "Rel-deficient", "T", "cells", "exhibit", "defects", "in", "production", "of", "interleukin", "3", "and", "granulocyte-macrophage", "colony-stimulating", "factor", "." ]

[ 0, 7, 8, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 10, 0 ]

[ "The", "c-rel", "protooncogene", "encodes", "a", "subunit", "of", "the", "NF-kappa", "B-like", "family", "of", "transcription", "factors", "." ]

[ 0, 1, 2, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0 ]

[ "Mice", "lacking", "Rel", "are", "defective", "in", "mitogenic", "activation", "of", "B", "and", "T", "lymphocytes", "and", "display", "impaired", "humoral", "immunity", "." ]

[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0 ]

[ "In", "an", "attempt", "to", "identify", "changes", "in", "gene", "expression", "that", "accompany", "the", "T-cell", "stimulation", "defects", "associated", "with", "the", "loss", "of", "Rel", ",", "we", "have", "examined", "the", "expression", "of", "cell", "surface", "activation", "markers", "and", "cytokine", "production", "in", "mitogen-stimulated", "Rel-/-", "T", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "The", "expression", "of", "cell", "surface", "markers", "including", "the", "interleukin", "2", "receptor", "alpha", "(", "IL-2R", "alpha", ")", "chain", "(", "CD25", ")", ",", "CD69", "and", "L-selectin", "(", "CD62", ")", "is", "normal", "in", "mitogen-activated", "Rel-/-", "T", "cells", ",", "but", "cytokine", "production", "is", "impaired", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 0, 9, 0, 0, 9, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "Rel-/-", "splenic", "T", "cell", "cultures", "stimulated", "with", "phorbol", "12-myristate", "13-acetate", "and", "ionomycin", ",", "the", "levels", "of", "IL-3", ",", "IL-5", ",", "granulocyte-", "macrophage", "colony-stimulating", "factor", "(", "GM-CSF", ")", ",", "tumor", "necrosis", "factor", "alpha", "(", "TNF-alpha", ")", ",", "and", "gamma", "interferon", "(", "IFN-gamma", ")", "were", "only", "2-", "to", "3-fold", "lower", "compared", "with", "normal", "T", "cells", "." ]

[ 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 9, 10, 10, 10, 0, 9, 0, 0, 9, 10, 10, 10, 0, 9, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "In", "contrast", ",", "anti-CD3", "and", "anti-CD28", "stimulated", "Rel-/-", "T", "cells", ",", "which", "fail", "to", "proliferate", ",", "make", "little", "or", "no", "detectable", "cytokines", "." ]

[ 0, 0, 0, 9, 0, 9, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Exogenous", "IL-2", ",", "which", "restitutes", "the", "proliferative", "response", "of", "the", "anti-CD3-", "and", "anti-CD28-treated", "Rel-/-", "T", "cells", ",", "restores", "production", "of", "IL-5", ",", "TNF-alpha", ",", "and", "IFN-gamma", ",", "but", "not", "IL-3", "and", "GM-CSF", "expression", "to", "approximately", "normal", "levels", "." ]

[ 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 9, 0, 9, 0, 0, 9, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0 ]

[ "In", "contrast", "to", "mitogen-activated", "Rel-/-", "T", "cells", ",", "lipopolysaccharide-stimulated", "Rel-/-", "macrophages", "produce", "higher", "than", "normal", "levels", "of", "GM-CSF", "." ]

[ 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "These", "findings", "establish", "that", "Rel", "can", "function", "as", "an", "activator", "or", "repressor", "of", "gene", "expression", "and", "is", "required", "by", "T", "lymphocytes", "for", "production", "of", "IL-3", "and", "GM-CSF", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 9, 0, 9, 0 ]

[ "Nuclear", "appearance", "of", "a", "factor", "that", "binds", "the", "CD28", "response", "element", "within", "the", "interleukin-2", "enhancer", "correlates", "with", "interleukin-2", "production", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 2, 0, 0, 9, 0, 0 ]

[ "Activation", "of", "T", "lymphocytes", "requires", "the", "combined", "signaling", "of", "the", "T", "cell", "receptor", "and", "costimulatory", "molecules", "such", "as", "CD28", "." ]

[ 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 0 ]

[ "The", "ability", "of", "T", "cells", "to", "produce", "interleukin-2", "(", "IL-2", ")", "is", "a", "critical", "control", "point", "in", "T", "lymphocyte", "activation", "." ]

[ 0, 0, 0, 7, 8, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0 ]

[ "The", "IL-2", "enhancer", "contains", "a", "functional", "motif", "named", "CD28", "response", "element", "(", "CD28RE", ")", "that", "serves", "a", "role", "as", "a", "target", "for", "mitogenic", "T", "cell", "activation", "signals", "." ]

[ 0, 1, 2, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0 ]

[ "The", "CD28RE", "sequence", "reveals", "similarity", "to", "the", "consensus", "kappaB", "binding", "motif", "." ]

[ 0, 1, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0 ]

[ "Here", "we", "demonstrate", "that", "CD28RE", "binds", "an", "inducible", "protein", "with", "a", "molecular", "mass", "of", "approximately", "35", "kDa", "called", "nuclear", "factor", "of", "mitogenic-activated", "T", "cells", "(", "NF-MATp35", ")", "that", "is", "clearly", "different", "from", "the", "known", "NF-", "kappaB/Rel", "family", "members", "." ]

[ 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "Induction", "of", "NF-MATp35", "was", "shown", "to", "depend", "on", "de", "novo", "protein", "synthesis", "and", "was", "restricted", "to", "T", "cells", "that", "received", "a", "mitogenic", "combination", "of", "T", "cell", "stimuli", ",", "not", "necessarily", "including", "CD28", "signaling", "." ]

[ 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "Nonmitogenic", "T", "cell", "stimulation", "did", "not", "result", "in", "appearance", "of", "NF-MATp35", "." ]

[ 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "These", "results", "indicate", "that", "mitogenic", "combinations", "of", "T", "cell", "activation", "signals", "are", "integrated", "at", "the", "level", "of", "NF-MATp35", "induction", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "Similar", "to", "its", "effect", "on", "IL-2", "production", ",", "cyclosporin", "A", "inhibited", "the", "induction", "of", "NF-MATp35", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "Taken", "together", ",", "these", "data", "demonstrate", "that", "the", "nuclear", "appearance", "of", "NF-MATp35", "shows", "excellent", "correlation", "with", "IL-2", "production", ",", "which", "is", "a", "unique", "characteristic", "among", "nuclear", "factors", "implicated", "in", "the", "control", "of", "IL-2", "gene", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0 ]

[ "Transcriptional", "basis", "for", "hyporesponsiveness", "of", "the", "human", "inducible", "nitric", "oxide", "synthase", "gene", "to", "lipopolysaccharide/interferon-gamma", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 0, 0, 0 ]

[ "The", "work", "reported", "here", "resolves", ",", "at", "the", "level", "of", "gene", "regulation", ",", "the", "controversy", "as", "to", "whether", "or", "not", "human", "monocytes/macrophages", "can", "produce", "nitric", "oxide", "(", "NO", ")", "when", "stimulated", "with", "lipopolysaccharide", "(", "LPS", ")", ",", "with", "or", "without", "co-stimulation", "by", "interferon-gamma", "(", "IFN-gamma", ")", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0 ]

[ "Studies", "included", "structural", "comparison", "of", "the", "promoters", "for", "human", "and", "mouse", "inducible", "NO", "synthase", "(", "iNOS", ")", "genes", ",", "transfection", "and", "assay", "of", "human", "and", "mouse", "iNOS", "promoter", "regions", "in", "response", "to", "LPS", "+/-", "IFN-gamma", ",", "and", "electrophoretic", "mobility", "shift", "assays", "of", "kappa", "B", "response", "elements", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]

[ "Two", "explanations", "for", "hyporesponsiveness", "of", "the", "human", "iNOS", "promoter", "to", "LPS", "+/-", "IFN-gamma", "were", "found", ":", "(", "1", ")", "multiple", "inactivating", "nucleotide", "substitutions", "in", "the", "human", "counterpart", "of", "the", "enhancer", "element", "that", "has", "been", "shown", "to", "regulate", "LPS/", "IFN-gamma", "induced", "expression", "of", "the", "mouse", "iNOS", "gene", ";", "and", "(", "2", ")", "and", "absence", "of", "one", "or", "more", "nuclear", "factors", "in", "human", "macrophages", "(", "e.g.", ",", "an", "LPS-inducible", "nuclear", "factor-kappa", "B/Rel", "complex", ")", ",", "that", "is", "(", "are", ")", "required", "for", "maximal", "expression", "of", "the", "gene", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "importance", "of", "resolution", "of", "this", "controversy", "is", "that", "future", "research", "in", "this", "area", "should", "be", "directed", "toward", "the", "understanding", "of", "alternative", "mechanisms", "that", "can", "result", "in", "the", "successful", "production", "of", "NO", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "X", "inactivation", "analysis", "in", "a", "female", "with", "hypomelanosis", "of", "Ito", "associated", "with", "a", "balanced", "X", ";", "17", "translocation", ":", "evidence", "for", "functional", "disomy", "of", "Xp", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "X", "inactivation", "analysis", "was", "performed", "on", "normal", "and", "hypopigmented", "skin", "samples", "obtained", "from", "a", "female", "with", "hypomelanosis", "of", "Ito", "associated", "with", "a", "balanced", "whole", "arm", "X", ";", "17", "translocation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Severe", "skewing", "of", "X", "inactivation", "resulting", "in", "inactivity", "of", "the", "intact", "X", "was", "found", "in", "blood", "and", "cultures", "of", "both", "types", "of", "skin", ",", "but", "analysis", "of", "DNA", "prepared", "directly", "from", "hypopigmented", "skin", "showed", "significant", "inactivation", "of", "the", "translocated", "X", ",", "inconsistent", "with", "the", "usual", "mechanism", "of", "phenotypic", "expression", "in", "X", ";", "autosome", "translocations", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "addition", ",", "dual", "colour", "FISH", "analysis", "using", "centromere", "specific", "probes", "for", "chromosomes", "X", "and", "17", "showed", "that", "the", "breakpoints", "on", "both", "chromosomes", "lie", "within", "the", "alphoid", "arrays", ",", "making", "interruption", "of", "a", "locus", "on", "either", "chromosome", "unlikely", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "While", "partial", "variable", "monosomy", "of", "loci", "on", "chromosome", "17p", "can", "not", "be", "excluded", "as", "contributing", "to", "the", "phenotype", "in", "this", "patient", ",", "it", "is", "argued", "that", "the", "major", "likely", "factor", "is", "partial", "functional", "disomy", "of", "sequences", "on", "Xp", "in", "cell", "lineages", "that", "have", "failed", "to", "inactivate", "the", "intact", "X", "chromosome", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]

[ "Identification", "of", "a", "herpesvirus", "Saimiri", "cis-acting", "DNA", "fragment", "that", "permits", "stable", "replication", "of", "episomes", "in", "transformed", "T", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "Herpesvirus", "saimiri", "is", "a", "lymphotropic", "herpesvirus", "capable", "of", "immortalizing", "and", "transforming", "T", "cells", "both", "in", "vitro", "and", "in", "vivo", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0 ]

[ "Immortalized", "and", "transformed", "T", "cells", "harbor", "several", "copies", "of", "the", "viral", "genome", "as", "a", "persisting", "genome", "." ]

[ 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "mapping", "of", "the", "cis-acting", "genetic", "cis-acting", "segment", "(", "oriP", ")", "required", "for", "viral", "episomal", "maintenance", "is", "reported", "here", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Viral", "DNA", "fragments", "that", "potentially", "contain", "oriP", "were", "cloned", "into", "a", "plasmid", "that", "contains", "the", "hygromycin", "resistance", "gene", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "After", "several", "round", "of", "subcloning", "followed", "by", "transfection", ",", "oriP", "was", "mapped", "to", "a", "1.955-kb", "viral", "segment", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "This", "viral", "fragment", "permits", "stable", "plasmid", "replication", "without", "deletion", "or", "rearrangement", "as", "well", "as", "episomal", "maintenance", "without", "integration", "or", "recombination", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "function", "of", "oriP", "depends", "on", "a", "trans-acting", "factor", "(", "s", ")", "encoded", "by", "the", "viral", "genome", "." ]

[ 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "1.955-kb", "viral", "segment", "includes", "a", "dyad", "symmetry", "region", "located", "between", "two", "small", "nuclear", "RNA", "genes", "and", "is", "located", "upstream", "of", "the", "dihydrofolate", "reductase", "gene", "homolog", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0 ]

[ "Therefore", ",", "this", "oriP", "contains", "novel", "elements", "distinct", "from", "those", "of", "other", "DNA", "viruses", "." ]

[ 0, 0, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "vivo", "anergized", "CD4+", "T", "cells", "express", "perturbed", "AP-1", "and", "NF-kappa", "B", "transcription", "factors", "." ]

[ 0, 0, 0, 0, 7, 8, 0, 0, 9, 0, 9, 10, 10, 10, 0 ]

[ "Anergy", "is", "a", "major", "mechanism", "to", "ensure", "antigen-specific", "tolerance", "in", "T", "lymphocytes", "in", "the", "adult", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0 ]

[ "In", "vivo", ",", "anergy", "has", "mainly", "been", "studied", "at", "the", "cellular", "level", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "this", "study", ",", "we", "used", "the", "T-cell-activating", "superantigen", "staphylococcal", "enterotoxin", "A", "(", "SEA", ")", "to", "investigate", "molecular", "mechanisms", "of", "T-lymphocyte", "anergy", "in", "vivo", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0 ]

[ "Injection", "of", "SEA", "to", "adult", "mice", "activates", "CD4+", "T", "cells", "expressing", "certain", "T-cell", "receptor", "(", "TCR", ")", "variable", "region", "beta-chain", "families", "and", "induces", "strong", "and", "rapid", "production", "of", "interleukin", "2", "(", "IL-2", ")", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0 ]

[ "In", "contrast", ",", "repeated", "injections", "of", "SEA", "cause", "CD4+", "T-cell", "deletion", "and", "anergy", "in", "the", "remaining", "CD4+", "T", "cells", ",", "characterized", "by", "reduced", "expression", "of", "IL-2", "at", "mRNA", "and", "protein", "levels", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0 ]

[ "We", "analyzed", "expression", "of", "AP-1", ",", "NF-kappa", "B", ",", "NF-AT", ",", "and", "octamer", "binding", "transcription", "factors", ",", "which", "are", "known", "to", "be", "involved", "in", "the", "regulation", "of", "IL-2", "gene", "promoter", "activity", "." ]

[ 0, 0, 0, 0, 9, 0, 9, 10, 0, 9, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0 ]

[ "Large", "amounts", "of", "AP-1", "and", "NF-kappa", "B", "and", "significant", "quantities", "of", "NF-AT", "were", "induced", "in", "SEA-activated", "CD4+", "spleen", "T", "cells", ",", "whereas", "Oct-1", "and", "Oct-2", "DNA", "binding", "activity", "was", "similar", "in", "both", "resting", "and", "activated", "T", "cells", "." ]

[ 0, 0, 0, 9, 0, 9, 10, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 7, 8, 8, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "In", "contrast", ",", "anergic", "CD4+", "T", "cells", "contained", "severely", "reduced", "levels", "of", "AP-1", "and", "Fos/Jun-containing", "NF-AT", "complexes", "but", "expressed", "significant", "amounts", "of", "NF-kappa", "B", "and", "Oct", "binding", "proteins", "after", "SEA", "stimulation", "." ]

[ 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 9, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 10, 0, 9, 10, 10, 0, 0, 0, 0 ]

[ "Resolution", "of", "the", "NF-kappa", "B", "complex", "demonstrated", "predominant", "expression", "of", "p50-p65", "heterodimers", "in", "activated", "CD4+", "T", "cells", ",", "while", "anergic", "cells", "mainly", "expressed", "the", "transcriptionally", "inactive", "p50", "homodimer", "." ]

[ 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 9, 10, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "These", "alterations", "of", "transcription", "factors", "are", "likely", "to", "be", "responsible", "for", "repression", "of", "IL-2", "in", "anergic", "T", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 7, 8, 0 ]

[ "Fas", "ligation", "induces", "apoptosis", "and", "Jun", "kinase", "activation", "independently", "of", "CD45", "and", "Lck", "in", "human", "T", "cells", "." ]

[ 9, 0, 0, 0, 0, 9, 10, 0, 0, 0, 9, 0, 9, 0, 7, 8, 8, 0 ]

[ "Stimulation", "through", "the", "Fas/APO-1", "receptor", "results", "in", "apoptosis", "through", "an", "incompletely", "characterized", "signaling", "pathway", "." ]

[ 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "More", "is", "known", "regarding", "signal", "transduction", "events", "that", "occur", "after", "ligation", "of", "the", "T-cell", "antigen", "receptor", "(", "TCR", ")", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0 ]

[ "It", "has", "been", "shown", "that", "TCR", "stimulation", "requires", "both", "the", "membrane", "tyrosine", "phosphatase", ",", "CD45", ",", "and", "the", "Src-family", "kinase", ",", "Lck", ",", "to", "result", "in", "cellular", "activation", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0 ]

[ "Although", "prior", "studies", "suggest", "a", "role", "for", "protein", "tyrosine", "kinases", "and", "phosphatases", "in", "Fas", "signaling", ",", "we", "report", "here", "that", "Fas", "ligation", "induces", "apoptosis", "in", "T", "cells", "deficient", "in", "either", "CD45", "or", "Lck", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 9, 0, 9, 0 ]

[ "Further", ",", "in", "normal", "and", "CD45-", "or", "Lck-deficient", "cell", "lines", ",", "Fas", "stimulation", "results", "in", "activation", "of", "Jun", "kinase", "(", "JNK", ")", ",", "a", "proposed", "mediator", "of", "stress", "activation", "pathways", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Previous", "studies", "have", "also", "demonstrated", "a", "role", "for", "endogenous", "ceramide", "release", "in", "Fas-mediated", "apoptosis", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "show", "that", "stimulation", "with", "a", "synthetic", "ceramide", "analog", "results", "in", "JNK", "activation", "as", "well", "as", "apoptosis", ",", "suggesting", "ceramide", "release", "occurs", "proximal", "to", "JNK", "activation", "in", "Fas", "signaling", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 9, 0, 0 ]

[ "Our", "data", "suggest", "that", "although", "CD45", "and", "Lck", "are", "not", "required", "for", "Fas", "signaling", ",", "JNK", "activation", "may", "play", "an", "important", "role", "transducing", "distal", "signals", "that", "lead", "to", "apoptosis", "after", "Fas", "ligation", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 9, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0 ]

[ "Globin", "gene", "switching", "." ]

[ 1, 2, 0, 0 ]

[ "In", "vivo", "protein-DNA", "interactions", "of", "the", "human", "beta-globin", "locus", "in", "erythroid", "cells", "expressing", "the", "fetal", "or", "the", "adult", "globin", "gene", "program", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 7, 8, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0 ]

[ "To", "characterize", "the", "protein-DNA", "interactions", "important", "for", "the", "developmental", "control", "of", "the", "human", "beta-globin", "locus", ",", "we", "analyzed", "by", "in", "vivo", "dimethyl", "sulfate", "footprinting", "erythroid", "cells", "expressing", "either", "the", "fetal", "or", "the", "adult", "globin", "developmental", "program", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0 ]

[ "In", "the", "locus", "control", "region", "(", "LCR", ")", "of", "the", "beta-globin", "locus", ",", "in", "vivo", "footprints", "on", "NF-E2", "(", "or", "AP-1", ")", "and", "GATA-1", "motifs", "remained", "the", "same", "regardless", "of", "whether", "the", "fetal", "or", "the", "adult", "globin", "genes", "are", "expressed", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 1, 0, 0, 1, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0 ]

[ "In", "contrast", ",", "in", "vivo", "footprints", "on", "GT", "(", "CACCC", ")", "motifs", "differed", "between", "the", "cells", "expressing", "the", "fetal", "or", "the", "adult", "globin", "program", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0 ]

[ "In", "promoter", "regions", ",", "the", "actively", "transcribed", "genes", "demonstrated", "extensive", "and", "consistent", "footprints", "over", "the", "canonical", "elements", ",", "such", "as", "CACCC", "and", "CCAAT", "motifs", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "adult", "globin", "expressing", "cells", "displayed", "more", "extensive", "footprints", "than", "the", "fetal", "globin", "expressing", "cells", "in", "the", "3", "'", "regulatory", "sequences", "of", "both", "the", "Agamma-", "and", "the", "beta-globin", "genes", ",", "suggesting", "a", "role", "of", "these", "3", "'", "elements", "in", "beta-globin", "gene", "expression", "." ]

[ 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0 ]

[ "Our", "results", "suggest", "that", "the", "bulk", "of", "protein-DNA", "interactions", "that", "underlies", "the", "developmental", "control", "of", "globin", "genes", "takes", "place", "in", "the", "gamma-", "and", "beta-globin", "gene", "promoters", ",", "and", "that", "GT", "motifs", "of", "the", "beta-globin", "locus", "LCR", "may", "play", "a", "role", "in", "the", "developmental", "regulation", "of", "human", "beta-globin", "gene", "expression", ",", "perhaps", "by", "increasing", "the", "probability", "of", "interaction", "of", "the", "LCR", "holocomplex", "with", "the", "fetal", "or", "the", "adult", "globin", "gene", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 1, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0 ]

[ "Antisense", "inhibition", "of", "vitamin", "D", "receptor", "expression", "induces", "apoptosis", "in", "monoblastoid", "U937", "cells", "." ]

[ 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 5, 6, 6, 0 ]

[ "The", "active", "vitamin", "D3", "metabolite", "1", ",", "25-dihydroxycholecalciferol", "(", "1", ",", "25", "(", "OH", ")", "2D3", ")", "acts", "as", "an", "antiproliferative", "and", "differentiating", "agent", "for", "the", "monoblastoid", "cell", "line", "U937", "and", "as", "an", "important", "immunologic", "mediator", "implicated", "particularly", "in", "the", "function", "of", "cells", "belonging", "to", "the", "monocyte/macrophage", "lineage", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 0 ]

[ "These", "effects", "are", "controlled", "by", "the", "vitamin", "D", "receptor", "(", "VDR", ")", ",", "a", "member", "of", "the", "steroid", "hormone", "receptor", "family", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "The", "objective", "of", "this", "study", "was", "to", "develop", "U937", "transfectants", "expressing", "antisense", "VDR", "mRNA", ",", "and", "to", "use", "these", "to", "examine", "the", "role", "of", "1", ",", "25", "(", "OH", ")", "2D3", "-VDR", "interaction", "in", "this", "lineage", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 5, 0, 0, 0, 3, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0 ]

[ "A", "2-kb", "VDR", "cDNA", "insert", "(", "including", "the", "complete", "VDR", "coding", "region", ")", "was", "cloned", "in", "an", "antisense", "orientation", "into", "the", "EBV", "episomal", "vector", "pMEP4", "under", "the", "control", "of", "an", "inducible", "promoter", "and", "transfected", "into", "U937", "." ]

[ 0, 1, 2, 2, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 0 ]

[ "The", "resultant", "cell", "line", ",", "DH42", ",", "was", "hygromycin", "resistant", ",", "contained", "VDR", "cDNA", ",", "expressed", "fewer", "VDRs", "than", "controls", ",", "and", "showed", "a", "substantial", "decrease", "in", "antiproliferative", "response", "to", "1", ",", "25", "(", "OH", ")", "2D3", "." ]

[ 0, 0, 0, 0, 0, 5, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "However", ",", "1", ",", "25", "(", "OH", ")", "2D3", "increased", "the", "number", "of", "cells", "expressing", "macrophage", "cell", "surface", "Ags", ",", "including", "CD14", "and", "CD11b", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 9, 0, 9, 0 ]

[ "A", "subpopulation", "of", "smaller", "cells", "did", "not", "express", "the", "differentiation", "markers", "after", "cadmium", "stimulation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]