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Rapid aromatic hydrogen exchange in the antimalarial primaquine.
Primaquine, an 8-aminoquinoline antimalarial, is shown to undergo unexpectedly rapid aromatic proton exchange with the medium. At a pH less than 4, the exchange of C-5 is so fast as to be unmeasurable by proton nmr methods. At a pH of 6-6.5 the half-time for exchange is 4 to 5 minutes. This unexpectedly high rate of exchange of a carbon-proton with medium may provide an important clue to the biological activity of primaquine. | {
"pile_set_name": "PubMed Abstracts"
} |
Thromboembolic events after arthroscopic knee surgery.
We present a case of fatal pulmonary embolus after knee arthroscopy to raise awareness of the frequency of this complication and the need for consideration of thromboembolic prophylaxis before knee arthroscopy. | {
"pile_set_name": "PubMed Abstracts"
} |
Protective effects of Glomus iranicum var. tenuihypharum on soil and Viburnum tinus plants irrigated with treated wastewater under field conditions.
Currently, irrigation using recycled water is increasing, especially in semiarid environments, but a potential problem of using reclaimed wastewater is its elevated salt levels. The application of arbuscular mycorrhizal fungi (AMF) could be a suitable option to mitigate the negative effects produced by the salinity. In this work, the combined effect of Glomus iranicum var. tenuihypharum and two types of water: Control, C, with EC <0.9 dS m(-1) and reclaimed water (wastewater previously treated in a sewage treatment plant) with EC 4 dS m(-1) during a first saline period (11 weeks) and with EC 6 dS m(-1) during a second saline period (25 weeks), was evaluated for laurustinus (Viburnum tinus) plants under field conditions. This plant is a popular shrub very used for gardening. Chemical properties of soil as well as physiological behavior, leaf nutrition, and esthetic value of plants were evaluated. Due to the high salinity from wastewater at 6 dS m(-1), laurustinus plants decreased their stem water potential values and, to a lesser extent, the stomatal conductance. Also, the visual quality of the plants was diminished. The inoculated AMF satisfactorily colonized the laurustinus roots and enhanced the structure of the soil by increasing the glomalin and carbon contents. Furthermore, G. iranicum var. tenuihypharum inoculation decreased Na and Cl content, stimulated flowering and improved the stem water potential of the plants irrigated with both types of reclaimed water. The AMF also had a positive effect as a consequence of stimulation of plant physiological parameters, such as the stem water potential and stomatal conductance. Effective AMF associations that avoid excessive salinity could provide wastewater reuse options, especially when the plants grow in soils. | {
"pile_set_name": "PubMed Abstracts"
} |
Structural and dynamical aspects of Streptococcus gordonii FabH through molecular docking and MD simulations.
β-Ketoacyl-ACP-synthase III (FabH or KAS III) has become an attractive target for the development of new antibacterial agents which can overcome the multidrug resistance. Unraveling the fatty acid biosynthesis (FAB) metabolic pathway and understanding structural coordinates of FabH will provide valuable insights to target Streptococcus gordonii for curing oral infection. In this study, we designed inhibitors against therapeutic target FabH, in order to block the FAB pathway. As compared to other targets, FabH has more interactions with other proteins, located on the leading strand with higher codon adaptation index value and associated with lipid metabolism category of COG. Current study aims to gain in silico insights into the structural and dynamical aspect of S. gordonii FabH via molecular docking and molecular dynamics (MD) simulations. The FabH protein is catalytically active in dimerization while it can lock in monomeric state. Current study highlights two residues Pro88 and Leu315 that are close to each other by dimerization. The active site of FabH is composed of the catalytic triad formed by residues Cys112, His249, and Asn279 in which Cys112 is involved in acetyl transfer, while His249 and Asn279 play an active role in decarboxylation. Docking analysis revealed that among the studied compounds, methyl-CoA disulfide has highest GOLD score (82.75), binding affinity (-11 kcal/mol) and exhibited consistently better interactions. During MD simulations, the FabH structure remained stable with the average RMSD value of 1.7 Å and 1.6 Å for undocked protein and docked complex, respectively. Further, crucial hydrogen bonding of the conserved catalytic triad for exhibiting high affinity between the FabH protein and ligand is observed by RDF analysis. The MD simulation results clearly demonstrated that binding of the inhibitor with S. gordonii FabH enhanced the structure and stabilized the dimeric FabH protein. Therefore, the inhibitor has the potential to become a lead compound. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of dietary trimethylamine on free amino acid and nonprotein nitrogen levels in muscle of the guppy, Poecilia reticulata, in relation to seawater adaptation.
Guppies Poecilia reticulata acclimated to 100% seawater (SW) had lower taurine and alanine levels in muscle than fish kept in freshwater (FW). The glycine level, in contrast, was higher in SW fish than in FW fish. Levels of other free amino acids (FAA) were comparatively low and little different between fish adapted in FW and in SW. In both FW and SW fish almost all of muscle FAA showed little difference in levels between fish kept on diets containing three different levels of trimethylamine (TMA) (0, 223, and 334 mumol TMA/kg dry weight of diet). Total FAA and nonprotein nitrogen levels in muscle were unaffected by the difference in either the diet species or the ambient salinities. Muscle trimethylamine oxide levels were higher in SW fish than in FW fish. In both salinities, muscle trimethylamine oxide levels in fish on the diets containing 223 and 334 mumol TMA/kg were slightly greater than the level in fish on the TMA-free diet. | {
"pile_set_name": "PubMed Abstracts"
} |
Analysis of hydroxylation and nitration products of D-phenylalanine for in vitro and in vivo radical determination using high-performance liquid chromatography and photodiode array detection.
D-phenylalanine is capable of trapping reactive oxygen species (ROS) and reactive nitrogen species (RNS) by forming three major hydroxylation (o-, m-, p-tyrosine) and two major nitration products (nitrophenylalanine, nitrotyrosine). Here, we show how a method for the analysis of these phenylalanine derivatives was established using isocratic HPLC (Nucleosil120, C18 column) coupled with photodiode array detection and validated for cell-free in vitro and in vivo determination of radical formation. An ideal separation was achieved using a mobile phase consisting of 5% acetonitrile, 50mM KH(2)PO(4), pH 3.0, a column temperature of 35 degrees C and a flow rate of 1.0 mL/min. Limits of detection were in the range of 5-100 nM. Linearity was given within 5 nM-100 microM (correlation coefficient >0.999). Retention times as well as peak heights exhibited a high precision (RSD: <or=0.1% and <1.5%, respectively). The feasibility of d-phenylalanine for ROS/RNS measurement was demonstrated in a cell-free in vitro assay using peroxynitrite and by analysis of brain samples of mice treated with the dopaminergic neurotoxin 6-hydroxydopamine. | {
"pile_set_name": "PubMed Abstracts"
} |
Nonpharmacologic therapy for hypertension: does it really work?
Nondrug therapy of hypertension really does work but requires strong motivation by both patient and physician. In addition to global health benefits, prescription of weight loss, exercise, moderation of salt and alcohol intake, Dietary Approach to Stop Hypertension (DASH) eating plan, and tobacco avoidance can decrease the risk for normotensive and prehypertensive patients of developing fixed hypertension. Initiating and maintaining a healthy lifestyle may be sufficient to avoid pharmacologic therapy for some patients and is a valuable adjunct to drug therapy for most. Blood pressure lowering can be achieved by weight reduction (5-20 mm Hg/10 kg), DASH eating plan (8-14 mm Hg), dietary sodium reduction (2-8 mm Hg), increased physical activity (4-9 mm Hg), and moderation of alcohol consumption (2-4 mm Hg). Combination of two or more modalities may have an additive benefit. Cessation of tobacco abuse not only has global health benefits, but may reduce blood pressure. | {
"pile_set_name": "PubMed Abstracts"
} |
Reproducibility and use of low-concentration skin prick test.
We aimed to evaluate the reproducibility of the skin prick test performed with serial 1:4 dilutions of commercial standardized extracts in comparison with serum-specific IgE and the undiluted commercial extract. Twenty-four subjects sensitized to one (17 cases) or two (seven cases) inhalant allergens were selected and submitted to duplicate skin prick tests with concentrated commercial allergenic extracts or with serial 1:4 dilutions of the same extracts in two different examinations 7 days apart. Blood samples were obtained from 17 of the 24 patients for specific IgE determination. No statistically significant within-patient variations in the area of the wheal in skin prick tests done 1 week apart were found up to the eighth dilution (1:256) of the commercial allergen. On a patient-by-patient basis, only some dilutions showed a statistically significant correlation between allergen-specific IgE and the wheal area elicited by the same allergen, and a significant correlation was found between the wheal elicited by 10 mg/ml histamine and both the concentrated and diluted allergens (up to the sixth dilution). In polysensitized patients, the allergen producing the largest wheal when used in concentrated form did not produce the same result when diluted. The skin prick test with low-potency allergens was reproducible in individual patients even after a 7-day interval up to a 1:256 dilution of the commercial extract, although there was no clear correlation with allergen-specific IgE concentration. In polysensitized patients, the use of high-potency or low-potency allergens for skin prick tests can lead to different conclusions regarding the relative importance of each allergen. | {
"pile_set_name": "PubMed Abstracts"
} |
Interleukin 2 receptor expression by activated HNK-1+ granular lymphocytes: a requirement for their proliferation.
A subpopulation of HNK-1+ cells was found to express interleukin 2 receptor (IL 2R) as monitored by the anti-Tac monoclonal antibody after lectin or allogeneic cell stimulation. When blood mononuclear cells were stimulated with phytohemagglutinin (PHA) or concanavalin A for 3 days, or with pokeweed mitogen or allogeneic mononuclear cells for 6 days, virtually all of the HNK-1+ cells remained as small resting lymphocytes. One-fourth of these HNK-1+ cells expressed IL 2R, however, usually within 18 hr after stimulation. Neither circulating HNK-1+ cells nor unstimulated HNK-1+ cells in short-term culture expressed IL 2R. Only the subset of HNK-1+ cells that expressed T cell surface markers, such as the sheep erythrocyte receptor and Leu-4 antigen, could be induced to express IL 2R. When long-term cultures of HNK-1+ cells were established with initial PHA stimulation and the continued presence of IL 2, a majority of the cells was found to express the IL 2R. When anti-Tac antibody was added to the long-term cultures, proliferation of the HNK-1+ cells was completely inhibited. These results suggest that entry into growth cycle by this subpopulation of HNK-1+ granular lymphocytes may require two signals: a relatively nonspecific ligand interaction with cell surface glycoprotein(s) to induce expression of IL 2R, and subsequent IL 2 interaction with these specific cell surface receptors. | {
"pile_set_name": "PubMed Abstracts"
} |
What's the Key to Unlocking the Proteasome's Gate?
In this issue of Structure, Bolten et al. (2016) describe the organization of the mycobacterial proteasome in complex with the ATP-independent bacterial proteasome activator (Bpa, PafE). They confirm several activation motifs employed by archaea and eukaryotes and highlight differences that pose Bpa as a novel architectural class of proteasome activators. | {
"pile_set_name": "PubMed Abstracts"
} |
Health maintenance organizations; basic health services; exclusion of in vitro fertilization--HRSA. Notice.
This notice of a decision by the Director, Office of Health Maintenance Organizations (OHMO), to allow federally qualified HMOs the option of excluding in vitro fertilization from the basic health services they are required to provide their members. | {
"pile_set_name": "PubMed Abstracts"
} |
[Prescribing inappropriate medication: the new STOPP/START criteria].
Prescribing inappropriate medication (PIM) is a common public health problem. Mainly due to associated adverse drugs events (ADE), it results in major morbidity and mortality, as well as increased healthcare utilization. For a long time, the systematic review of medications prescribed appeared as a solution for limiting PIM and the ADE associated with such prescriptions. With this aim and since 2008, the list of STOPP-START criteria has appeared as attractive in its design, as well as logical and easy to use. The initial version has just been updated and improved. After having detailed all improvements provided to the 2008 version, we present the result of its adaptation into French language by a group of French-speaking expert from Belgium, Canada, France, and Switzerland. | {
"pile_set_name": "PubMed Abstracts"
} |
First Trimester Laparoscopic Cerclage.
To review the indications, rationale, and technique for abdominal cerclage, specifically focusing on a laparoscopic approach to this procedure during the first trimester of pregnancy. This is an educational video directed toward gynecologic surgeons. Patient consent was obtained for use of surgical video footage, and Institutional Review Board exemption was granted. A patient case is discussed and a step-by-step description of the technique used to perform laparoscopic cerclage in the first trimester of pregnancy is demonstrated using surgical footage. The estimated incidence of cervical insufficiency affecting pregnancy is as high as 1%. Cervical cerclage placement is the treatment for this condition. Although most cerclages are placed transvaginally via the Shirodkar or McDonald technique, abdominal cerclage is necessary in women with a previous failed transvaginal cerclage or in those with minimal cervical tissue accessible vaginally [1,2]. Both laparoscopic and robotic approaches to this procedure have been developed, allowing patients to enjoy a more rapid recovery as well as to avoid an unnecessary laparotomy[3-6]. The observational studies reporting outcomes for laparoscopic-assisted abdominal cerclage quote fetal survival rates of >85%, which is comparable to the rates for abdominal cerclage[7-18]. Complication rates are low, also congruent with the laparotomic approach[12-18]. The patient, a 35-year-old gravida 3, para 1, 0, 1, 1, at 11 weeks gestation, had a history of a full-term vaginal delivery followed by an excisional procedure for cervical dysplasia, and then an early second trimester pregnancy loss. She was referred for laparoscopic-assisted abdominal cerclage after a severely shortened cervix was noted on examination. Laparoscopic cerclage placement was uncomplicated, with minimal blood loss encountered. The patient did well in the immediate postoperative period and was discharged home on postoperative day 1. The remainder of the pregnancy was uneventful, and she delivered via scheduled cesarean section at term. With proper patient selection and operative planning, the technique of laparoscopic cerclage is both safe and advantageous in terms of faster recovery. Obstetric outcomes are equivalent, if not superior, to an open abdominal approach to this procedure. | {
"pile_set_name": "PubMed Abstracts"
} |
[Morphometric analysis of a mast cell population].
A composite morphometric approach to the analysis of mast cell population is proposed including 4 criteria: the cytogram, the index of heparin saturation, degranulation index, a relative rate of degranulation form. The sensitivity and informative capacity of the composite morphometric analysis were tested in two experimental models: inoculation of heparin and inoculation of its antagonists. Subtle differences in the effect of mast cell degranulaton were found after inoculation of protamine sulphate and a synthetic polycation, 2.5-ionen. Important information on the consumption of exogenous heparin by mast cells was obtained. The simplicity of the analysis as well as the possibility of using its simplified version make the method available for both clinical and experimental pathology. | {
"pile_set_name": "PubMed Abstracts"
} |
Immunoglobulin G and M hepatitis C virus core antibody (JCC.2) response in chimpanzees infected experimentally with hepatitis C virus.
To evaluate changes in serum immunoglobulin M (IgM) and G (IgG) hepatitis C virus (HCV) core antibodies after HCV infection in acute hepatitis C. Serum HCV RNA and IgM and IgG HCV core antibodies were investigated using sera sequentially sampled from three chimpanzees experimentally infected with HCV. Serum IgG HCV core antibody titer was measured using a JCC.2 enzyme-linked immunosorbent assay (ELISA) kit (Chemo-Sera-Therapeutic Research Center, Kumamoto, Japan). IgM core antibody titer was measured using horseradish peroxidase-labeled monoclonal anti-human IgM as the secondary antibody for the JCC.2 ELISA kit. Serum HCV RNA was detected using the 5' noncoding region as the primer according to the reverse transcriptase (RT) nested polymerase chain reaction (PCR) and competitive RT-PCR method. IgM JCC.2 antibody was detected when alanine aminotransferase (ALT) peaked, showing the closest correlation with the changes in ALT. A period during which IgM JCC.2 antibody was positive but HCV RNA as determined by RT-nested PCR was negative was observed after the elevation of ALT level. These results indicate the usefulness of detection of serum IgM JCC.2 antibody in making a definitive diagnosis of acute hepatitis C and the follow-up observation of hepatitis C. | {
"pile_set_name": "PubMed Abstracts"
} |
Steroidogenic acute regulatory protein binds cholesterol and modulates mitochondrial membrane sterol domain dynamics.
The steroidogenic acute regulatory protein (StAR) mediates the rate-limiting step of steroidogenesis, delivery of cholesterol to the inner mitochondrial membrane. However, the mechanism whereby cholesterol translocation is accomplished has not been resolved. Recombinant StAR proteins lacking the first N-terminal 62 amino acids comprising the mitochondrial-targeting sequence were used to determine if StAR binds cholesterol and alters mitochondrial membrane cholesterol domains to enhance sterol transfer. First, a fluorescent NBD-cholesterol binding assay revealed 2 sterol binding sites (K(d) values near 32 nm), whereas the inactive A218V N-62 StAR mutant had only a single binding site with 8-fold lower affinity. Second, NBD-cholesterol spectral shifts and fluorescence resonance energy transfer from StAR Trp residues to NBD-cholesterol showed (i) close molecular interaction between these molecules (R(2/3) = 33 A) and (ii) sensitized NBD-cholesterol emission from only one of the two sterol binding sites. Third, circular dichroism showed that cholesterol binding induced a change in StAR secondary structure. Fourth, a fluorescent sterol transfer assay that did not require separation of donor and acceptor mitochondrial membranes demonstrated that StAR enhanced mitochondrial sterol transfer as much as 100-fold and induced/increased the formation of rapidly transferable cholesterol domains in isolated mitochondrial membranes. StAR was 67-fold more effective in transferring cholesterol from mitochondria of steroidogenic MA-10 cells than from human fibroblast mitochondria. In contrast, sterol carrier protein-2 (SCP-2) was only 2.2-fold more effective in mediating sterol transfer from steroidogenic cell mitochondria. Taken together these data showed that StAR is a cholesterol-binding protein, preferentially enhances sterol transfer from steroidogenic cell mitochondria, and interacts with mitochondrial membranes to alter their sterol domain structure and dynamics. | {
"pile_set_name": "PubMed Abstracts"
} |
Portal pumping: a new perspective for treatment of variceal hemorrhage and liver failure in end-stage cirrhosis?
In end-stage cirrhosis complicated by variceal hemorrhage, attempts to reduce portal pressure by treatments such as portosystemic shunts also decrease sinusoidal perfusion and risk impairing liver function. It has been suggested that encouraging portal flow to pass through the cirrhotic liver by mechanical action could cause a decrease in distal (splanchnic) portal pressure on one hand, and improve liver function on the other. The aim of this work was to evaluate the hemodynamic and functional effects of a 30-min pump-driven increase in portal blood flow through the liver of patients with end-stage cirrhosis before the anhepatic phase of liver transplantation. Basel portal flow (800 +/- 270 ml.min-1) was increased two fold (n = 10) or four fold (n = 9). When the flow was doubled, splanchnic portal pressure decreased 17.9 +/- 11.3% (from 31.8 +/- 5.3 to 26.0 +/- 5.8 mmHg, n = 10; p < 0.001); when flow was increased four fold, splanchnic portal pressure decreased 39.2 +/- 15.4% (from 32.8 +/- 5.0 to 19.9 +/- 6.0 mmHg, n = 9; p < 0.001). The comparison of indocyanine green clearance between basal and doubled portal flow demonstrated an increase of 32.1 +/- 26.9% (n = 5; p = 0.053). Histological analysis demonstrated sinusoidal dilatation in three out of ten livers. These results, as well as previous studies using isolated perfused cirrhotic rat or human livers, suggest that portal pumping should be explored as a treatment for certain sclerotherapy-resistant cirrhotic patients, with variceal hemorrhage and liver failure. | {
"pile_set_name": "PubMed Abstracts"
} |
Anticomplementary activity in human semen and its possible importance in reproduction.
We demonstrated anticomplementary activity on once-washed human sperm, and in normal and vasectomized seminal plasmas. It was demonstrated to be a normal component of human semen. The origin of the activity is proposed to be the seminal plasma with sperm adsorption of activity. The properties of the seminal anticomplementary factor were characterized further, and the molecular size was shown to be less than 3500 daltons. Reduced anticomplementary activity was found to be associated significantly with abnormal semen profiles and infertility in males. The activity in seminal plasma was shown to have no effect on complement-dependent sperm immobilizing antibodies in the serum of an infertile woman, implicating an effect on the post-C3 components of the complement pathway. The inhibition of complement-dependent haemolysis and the lack of inhibition of complement-dependent sperm immobilization by the anticomplementary factor are considered in the implications of the role of seminal anticomplementary activity in reproduction. | {
"pile_set_name": "PubMed Abstracts"
} |
Expression and activity of cAMP phosphodiesterase isoforms in pulmonary artery smooth muscle cells from patients with pulmonary hypertension: role for PDE1.
Pulmonary hypertension (PHT) is associated with increased vascular resistance due to sustained contraction and enhanced proliferation of pulmonary arterial smooth muscle cells (PASMC); the abnormal tone and remodeling in the pulmonary vasculature may relate, at least in part, to decreased cyclic nucleotide levels. Cyclic nucleotide phosphodiesterases (PDEs), of which 11 families have been identified, catalyze the hydrolysis of cAMP and cGMP. We tested the hypothesis that PASMC isolated from patients with PHT, either idiopathic pulmonary arterial hypertension (IPAH) or secondary pulmonary hypertension (SPH), have increased expression and activity of PDE isoforms that reduce the responsiveness of agents that raise cellular cAMP. Real-time PCR and immunoblotting demonstrated that the expression of PDE1A, PDE1C, PDE3B, and PDE5A was enhanced in PASMC from both IPAH and SPH patients compared with control PASMC. Consistent with this enhanced expression of PDEs, agonist-stimulated cAMP levels were significantly reduced in IPAH and SPH PASMC unless a PDE inhibitor was present. The use of specific PDE inhibitors revealed that an increase in PDE1 and PDE3 activity largely accounted for reduced agonist-induced cAMP levels and increased proliferation in IPAH and SPH PASMC. Treatment with PDE1C-targeted small interference RNA enhanced cAMP accumulation and inhibited cellular proliferation to a greater extent in PHT PASMC than controls. The results imply that an increase in PDE isoforms, in particular PDE1C, contributes to decreased cAMP and increased proliferation of PASMC in patients with PHT. PDE1 isoforms may provide novel targets for the treatment of both primary and secondary forms of the disease. | {
"pile_set_name": "PubMed Abstracts"
} |
Lymphoma and tuberculosis: temporal evolution of dual pathology on sequential 18F-FDG PET/CT.
Tuberculosis can often be seen in patients undergoing chemotherapy for lymphoma, especially in endemic countries. As both tuberculosis and lymphoma can lead to hypermetabolic lesions of F-FDG PET/CT, a diagnostic dilemma often ensues. We present the sequential F-FDG PET/CT images of a 22-year-old female patient with Hodgkin lymphoma who developed tuberculosis and later relapse of lymphoma. These images present the temporal evaluation of the dual pathology on F-FDG PET/CT. | {
"pile_set_name": "PubMed Abstracts"
} |
Purification and characterization of angiotensin converting enzyme-II from bovine seminal plasma.
Angiotensin converting enzyme (EC 3.4.15.1) from bovine seminal plasma was shown to exist in two distinct forms. A lower molecular weight form of the enzyme having higher activity was purified to homogeneity. Final recovery of the enzyme was 18.37%. The apparent molecular weight of the enzyme was estimated to be 1.99 x 10(5) by gel filtration. A value of 1.8 x 10(5) was obtained for the reduced and denatured enzyme by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Stokes' radius, diffusion coefficient and intrinsic viscosity were determined to be 51.89 A degree, 4.2 x 10(-7) cm2/sec and 4.42 cc/g, respectively. Chloride ions were required for the enzyme activity. Studies with EDTA suggest that metal ions which are tightly bound, are required for its activity. Enzyme was inhibited by some heavy metal ions and required disulfide linkages at its active site. Trypsin treatment of the urea denatured enzyme produced a catalytically active Mr 32,000 daltons fragment. | {
"pile_set_name": "PubMed Abstracts"
} |
[Value of absorbable clips in laparoscopic cholecystectomy. A randomized prospective study].
Most surgeons use metal clips in laparoscopic cholecystectomy. The aim of this prospective randomized controlled study was to evaluate the efficacy of absorbable clips in elective laparoscopic cholecystectomy. One hundred consecutive patients with symptomatic gallstones without complications were randomized into groups; group T had two metal clips (titan clip ETHICONR), group R (laproclipR Davis and Geck) had one absorbable clip applied on the cystic duct and cystic artery. The patients were followed for one year. There was no difference between the two groups concerning operative time, hospital stay and postoperative complications. The absorbable clips seem to be as effective as metal clips in providing hemostasis in cystic artery and in cystic duct ligation. | {
"pile_set_name": "PubMed Abstracts"
} |
Transport of alkali cations through thin lipid membranes by (222)C10-cryptand, an ionizable mobile carrier.
The kinetics of K+ and Na+ transport across the membrane of large unilamellar vesicles (L.U.V.) were compared at two pH's, with two carriers: (222)C10-cryptand (diaza-1,10-decyl-5-hexaoxa-4,7,13,16,21,24-bicyclo[8.8.8.]+ ++hexacosane) and valinomcyin, i.e. an ionizable macrobicyclic amino polyether and a neutral macrocyclic antibiotic. The rate of cation transport by (222)C10 saturated as cation and carrier concentrations rose. The apparent affinity of (222)C10 for K+ was higher and less pH dependent than that for Na+ but resembled the affinity of valinomycin for K+. The efficiency of (222)C10 transport of K+ decreased as the pH fell and the carrier concentration rose, and was about ten times lower than that of valinomycin. Noncompetitive K+/Na+ transport selectivity of (222)C10 decreased as pH, and cation and carrier concentrations rose, and was lower than that of valinomycin. Transport of alkali cations by (222)C10 and valinomycin was noncooperative. Reaction orders in cation n(S) and carrier m(M) varied with the type of cation and carrier and were almost independent of pH; n(S) and m(M) were not respectively dependent on carrier or cation concentrations. The apparent estimated constants for cation translocation by (222)C10 were higher in the presence of Na+ than of K+ due to higher carrier saturation by K+, and decreased as pH and carrier concentration increased. Equilibrium potential was independent of the nature of carrier and transported cation. Results are discussed in terms of the structural, physiocochemical and electrical characteristics of carriers and complexes. | {
"pile_set_name": "PubMed Abstracts"
} |
[Study on the chemical constituents of Sarcandra glabra by HPLC-ESI-MS/MS].
To study and compare the main chemical constituents of Sarcandra glabra and qingrexiaoyanning capsules which were extracted by acetic ether. The sample solution were analyzed by a Zorbax C18 column with a gradient mobile phase composed of acetonitrile and 0.1% formic acid solution. Both UV and mass spectrometry detector were used simutaneously, full-scan detection mode was evaluated for the identification of all LC peaks. We analyzed the mass spectrum of every LC peak and identified 26 molecular mass from the ion chromatogram of Sarcandra glabra extraction and 16 molecular mass from the extractions of qingrexiaoyanning capsule. 5 compounds were identified. High performance liquid chromatography-mass/mass spectrometry has special advantages on analyzing the chemical constituents of traditional Chinese medicine. | {
"pile_set_name": "PubMed Abstracts"
} |
Clinical outcomes in transition program for older adults with hip fracture.
Measuring and managing outcomes across the continuum of care has been a major task for healthcare organizations over the past decade. Care of older adults with hip fracture and their transition to the community is particularly challenging. This article describes a program designed specifically to meet these challenges. The goals of the transition program were to promote improved clinical outcomes, reduce acute care resource utilization through early discharge and provision of cost-effective home care, and maintain or improve patient satisfaction. Results of the outcome analysis demonstrated successful goal attainment. The strength of the outcome evaluation is that it links both a qualitative and quantitative approach, providing a richer and more holistic view of the client experience. | {
"pile_set_name": "PubMed Abstracts"
} |
Analysis of selective gene activation in yeast by differential display.
TATA box binding protein-associated factors (TAF(II)s) are dispensable for transcription of most genes in yeast. To further investigate the in vivo functions of TAF(II)s, differential display was used to identify a small subset of yeast genes whose transcription is dependent on yeast TAF(II)145, the core TAF(II) component that contacts TATA box-binding protein (TBP). Messenger RNA profiles derived from a wild-type TAF(II)145 strain and a temperature-sensitive taf(II)1145 strain were analyzed. Those genes whose messenger RNA level was greatly reduced in the temperature-sensitive taf(II)145 strain were cloned and further characterized. The procedure for performing differential display described here is modified from protocols provided by the manufacturer (Display Systems) and optimized for the yeast system. | {
"pile_set_name": "PubMed Abstracts"
} |
Salivary auto-antibodies as noninvasive diagnostic markers of oral cavity squamous cell carcinoma.
Oral cavity squamous cell carcinoma (OSCC) is one of the most common cancers worldwide, and its incidence is still increasing. Approximately 50% of patients with OSCC die within 5 years after diagnosis, mostly ascribed to the fact that the majority of patients present advanced stages of OSCC at the time of diagnosis. To discover salivary biomarkers for ameliorating the detection of OSCC, herein, we developed a multiplexed bead-based platform to simultaneously detect auto-antibodies (auto-Abs) in salivary samples. Compared with healthy individuals, the salivary levels of anti-p53, anti-survivin, anti-Hsp60, and anti-RPLP0 were significantly elevated in patients with OSCC. Noteworthily, the elevated levels of anti-p53, anti-survivin, and anti-Hsp60 were already observed in individuals with oral potentially malignant disorder. Moreover, the salivary levels of anti-p53, anti-survivin, anti-Hsp60, anti-RPLP0, and anti-CK8 were significantly elevated in patients with early-stage OSCC compared with those in healthy individuals. Most importantly, the use of a combined panel of salivary anti-p53, anti-survivin, anti-Hsp60, and anti-RPLP0 largely improves the detection of OSCC. Collectively, our results reveal that the salivary auto-Abs are effective OSCC biomarkers and the four-auto-Ab panel provides a novel and practicable approach for OSCC screening. This study provides the first evidence for the potential clinical application of salivary auto-Abs in OSCC diagnosis. | {
"pile_set_name": "PubMed Abstracts"
} |
Stable expression of human H1-histamine-receptor cDNA in Chinese hamster ovary cells. Pharmacological characterisation of the protein, tissue distribution of messenger RNA and chromosomal localisation of the gene.
A cDNA clone for the histamine H1 receptor was isolated from a human lung cDNA library; it encoded a protein of 487 amino acids which showed characteristic features of G-protein-coupled receptors. The percentages of identity of the deduced amino acid sequence with bovine, rat and guinea pig H1 histamine receptors were 82.6%, 79.4% and 73.3%, respectively, whereas these percentages decreased to 74.6%, 66% and 56.7% for the amino acid sequence of the third intracellular loop. The human H1-receptor cDNA was transfected into Chinese hamster ovary cells (CHO) via an eukaryotic expression vector; the receptor protein present on cell membranes specifically bound [3H]mepyramine with a Kd of 3.7 nM. The binding was displaced by H1-histamine-receptor antagonists and histamine. Northern blot analysis indicated the presence of two histamine H1 receptor mRNAs of 3.5 kb and 4.1 kb in various human tissues and an additional mRNA of 4.8 kb restricted to the human brain. Finally, by means of somatic cell hybrids segregating either human or rat chromosomes, the gene for histamine H1 receptor was found to reside on human chromosome 3 and rat chromosome 4. | {
"pile_set_name": "PubMed Abstracts"
} |
External electrostatic interaction versus internal encapsulation between cationic dendrimers and negatively charged drugs: which contributes more to solubility enhancement of the drugs?
Relationships of electrostatic interaction and encapsulation between poly(amidoamine) (PAMAM) dendrimers and negatively charged drug molecules have been investigated by aqueous solubility and NMR ( (1)H NMR and two-dimensional nuclear Overhauser effect spectroscopy (2D-NOESY)) studies. PAMAM dendrimers significantly increased the solubilities of phenobarbital and sulfamethoxazole, but scarcely influenced those of primidone and trimethoprim. Moreover, (1)H NMR and 2D-NOESY measurements indicated that few phenobarbital or sulfamethoxazole molecules were entrapped in the cavities of low-generation dendrimers (generation 3, G3). These results suggest that external electrostatic interaction contributes more to the solubility enhancement of drugs than internal encapsulation. | {
"pile_set_name": "PubMed Abstracts"
} |
Outcome of acute leukemia relapsing after bone marrow transplantation: utility of second transplants and adoptive immunotherapy.
We studied 231 acute leukemia patients relapsing after allogeneic (n = 114) or autologous (n = 117) BMT to assess the outcome of further therapy. In general, all patients in good condition were eligible for second transplants except for post-allograft relapses from 1993-1994 onwards who received cytokine- or cell-mediated immunotherapy. The major reason for patients not progressing to second graft was death from progressive disease or toxicity of salvage chemotherapy. Seventeen of 231 patients (7%) were alive at the last follow-up. Six of 14 post-autograft relapses treated with second transplants were alive and well, compared with five of 103 not undergoing second grafts (P < 0.0001). One of 23 post-allograft recipients treated with second allografts was alive with an extramedullary relapse, compared with five of 13 receiving immunotherapy and none of 78 receiving standard-dose or palliative therapy (P < 0.0001). We conclude that only a small proportion of highly selected acute leukemia patients relapsing after a transplant reach the stage of a conventional second transplant. In our experience, second allografts after myeloablative therapy in patients relapsing after one allograft are associated with very poor results, and immunotherapy may be a better approach in such cases. Selected patients relapsing after an autograft may become long-term survivors following a second autograft or an allograft. | {
"pile_set_name": "PubMed Abstracts"
} |
Portable mass spectrometry for measurement of anaesthetic agents and methane in respiratory gases.
Monitoring the composition of gases breathed by anaesthetised patients requires measurement methods with fast responses, high accuracy and good reliability. There is also an increasing demand for systems to be able to monitor more than one target analyte simultaneously, but some gas analysers can be sensitive to the presence of methane gas in exhaled breath, consequently leading to inaccurate measurements of the anaesthetic agent. This study investigated the feasibility of employing portable quadrupole mass spectrometry to monitor volatile anaesthetic agents (halothane, isoflurane and sevoflurane), methane accumulation in anaesthetic rebreathing systems, and inspired and exhaled carbon dioxide and oxygen concentrations during equine anaesthesia in a clinical setting. The volatile anaesthetic agents were easily measurable and methane was detectable. The instrument had an advantage over short wavelength infrared absorption spectrometry analysers because it could monitor anaesthetic agents and other respiratory gases simultaneously and at extremely low concentrations, although further optimisation is required. | {
"pile_set_name": "PubMed Abstracts"
} |
Polymerase chain reaction for detection of Clostridium botulinum types A, B and E in food, soil and infant faeces.
The application of the polymerase chain reaction (PCR) for detection of Clostridium botulinum types A, B and E in foods, environmental and clinical samples was evaluated and compared to the mouse bioassay. Samples inoculated with 10, 100 and 1000 spores of Cl. botulinum types A and B included pasteurized milk, UHT milk, infant formula, infant faeces, meat juice, canned tuna, mushrooms, blood sausage and soil. Clostridium botulinum type E spores were inoculated into fish eggs, canned tuna, picked herring, raw fish and soil at similar levels. Spores were added to 2.5 g of each sample with the exception of soil which was inoculated in 10 g samples. The presence of Cl. botulinum in sample enrichments was determined by both PCR and the bioassay. An overall correlation of 95.6% was observed between PCR results and the mouse bioassay. Of the total of 114 samples tested there was disparity between the mouse bioassay and the PCR in three samples of soil inoculated with 100 type A or E spores and 10 type B spores per 10 g, respectively, and two samples of infant faeces inoculated with 10 type A or B spores per 2.5 g. All of these samples gave negative animal results and positive PCR results. | {
"pile_set_name": "PubMed Abstracts"
} |
Dynamics and dynamic modelling of H2S production in sewer systems.
Accurate and reliable predictions of sulfide production in a sewer system greatly benefit formulation of appropriate strategies for optimal sewer management. Sewer systems, rising main systems in particular, are highly dynamic in terms of both flow and wastewater composition. In order to get an insight in sulfide production as a response to the dynamic changes in sewer conditions, several measurement campaigns were conducted in two rising mains in Gold Coast, Australia. The levels of various sulfur species and volatile fatty acids (VFAs) were monitored through hourly sampling for periods ranging from 8 to 29 h. The results of these field studies showed large temporal as well as spatial variations in sulfide generation. A dynamic sewer model taking into account the hydraulics and the biochemical transformation processes was formulated and calibrated and validated using the data collected during the four measurement campaigns at the two sites. The model was demonstrated to reasonably well describe the temporal and spatial variations in sulfide, sulfate and VFA concentrations. Application of the model was illustrated with a case study aimed to optimize oxygen injection to one of the two mains studied, which is being used as a means to control sulfide production on this site. The model predicted that, moving the current oxygen injection point to a location close to the end of the sewer line could achieve the same degree of sulfide control with only 50% of the current oxygen use. This study highlighted that the location at which oxygen is injected plays a major role in sulfide control and a dynamic model could be used to make a proper choice of the location. | {
"pile_set_name": "PubMed Abstracts"
} |
[Preoperative gastric acid secretion of gastric cancer patients and their prognosis].
This study was performed to evaluate how preoperative gastric acid secretion in gastric cancer patients correlated their prognosis. The results are as follows. 1) The 5-year survival rates of patients with MAO = 0mEq/h, 0mEq/h less than MAO less than or equal to 7.0mEq/h and 7.0mEq/h less than MAO were 29.2%, 73.6% and 88.4%, respectively. There were significant differences among them. 2) The patients with 7.0mEq/h less than MAO had normal cellular immunity and low immunosuppressive glycoproteins in sera. These results suggest that gastric cancer patients who have a potency of normal gastric acid secretion with normal cellular immunity, or who have a potency of normal immunity with normal gastric acid secretion, have a good prognosis. | {
"pile_set_name": "PubMed Abstracts"
} |
HLA-B27-restricted CD8 T cells derived from synovial fluids of patients with reactive arthritis and ankylosing spondylitis.
Ankylosing spondylitis and seronegative spondylarthropathies such as Reiter's syndrome and reactive arthritis are strongly associated with HLA-B27. However, the mechanisms by which HLA-B27 is involved in disease susceptibility and pathogenesis are unknown. If the disease association is a consequence of HLA-B27's physiological function in antigen presentation, the disease should be mediated by cytotoxic T lymphocytes (CTLs) that recognise bacterial or self peptides presented by HLA-B27. Proof of this arthritogenic peptide model requires isolation of B27-restricted CD8 T cells from arthritic joints of patients with spondylarthropathies. An important question is whether "arthritogenic" bacteria such as yersinia or salmonella can generate HLA-B27-restricted bacteria-specific CTLs. We describe such HLA-B27-restricted CTLs. We tested a panel of 354 alpha beta-TCR CD8 T lymphocyte clones (TLCs) that had been derived from the synovial fluid of 4 patients with reactive arthritis and 2 patients with ankylosing spondylitis. In 1 patient with yersinia-induced arthritis, 2 TLCs were identified that killed specifically yersinia-infected B27 target cells. In another patient with salmonella-induced arthritis, 1 B27-restricted CD8 TLC that recognised both salmonella and yersinia was identified. In 5 of the 6 patients autoreactive CTLs were found, 5 of which showed B27-restricted killing of uninfected cell lines. B27-restricted CTLs with specificity for arthritogenic bacteria or autoantigens provide a missing link in the pathogenesis of the HLA-B27-associated spondylarthropathies. | {
"pile_set_name": "PubMed Abstracts"
} |
[Catheter jejunostomy in elective abdominal surgery].
Needle catheter jejunostomy is used to a varying extent today. Therefore, the need for nutritional support was evaluated following elective abdominal surgery and compared to experiences with postoperative enteral feeding. Oesophagectomy, total gastrectomy and the Whipple procedure were identified as good indications for catheter jejunostomy, whereas with other types of operation an individual decision is required. | {
"pile_set_name": "PubMed Abstracts"
} |
Computerized electroencephalogram monitoring and carotid artery shunting.
During carotid artery surgery, ischemic electroencephalogram (EEG) changes were detected by computerized analysis in 27 of 225 carotid clamping intervals. All but 7 of these changes resolved spontaneously before the restoration of blood flow. Restored flow was associated, after variable delay, with an improved EEG pattern in 6 of 7 cases. Of 27 EEG episodes, there were 5 cases in which the EEG event persisted for 10 minutes or longer. All 5 patients developed new neurological deficits; none of the other patients did. It is suggested that all patients undergoing carotid artery surgery be monitored with EEG and that a shunt be placed if a persistent EEG change is noted. | {
"pile_set_name": "PubMed Abstracts"
} |
Erythroid gene suppression by NF-kappa B.
NF-kappa B/Rel transcription factors play essential roles to mediate the immune response and apoptosis, and they have also been implicated in cellular differentiation such as erythropoiesis. To elucidate the possible role(s) of NF-kappa B in erythroid gene regulation and erythropoiesis, we have carried out transient transfection studies of the human embryonic/fetal erythroid cell line K562 and mouse adult erythroid MEL cells. It is shown that tumor necrosis factor-alpha represses the transcription activity directed by either alpha or zeta globin promoter in a dose-dependent manner. Furthermore, different NF-kappa B family members could effectively repress the transfected alpha-like globin promoters in K562 as well as in MEL cells. The involvement of NF-kappa B pathway is supported by the ability of a NF-kappa B-specific, dominant negative mutant to block the tumor necrosis factor-alpha or p65-mediated suppression of the alpha-like globin promoter activities. The suppression appears to be mediated through cis-linked HS-40 enhancer. Finally, stably transfected K562 cells overexpressing p65 contain reduced amounts of the p45/NF-E2 RNA and functional NF-E2 proteins. Our studies have identified a new set of targets of NF-kappa B. We suggest that the relatively high activity of the NF-kappa B pathway in early erythroid progenitors is involved in the suppression of erythroid-specific genes. Later in differentiation, together with other changes, the decline of the amounts of the NF-kappa B family of factors leads to derepression and consequent increase of NF-E2, which in turn would activate a subset of erythroid-specific genes. | {
"pile_set_name": "PubMed Abstracts"
} |
The amino acid requirements of growing turkeys. 1. Model construction and parameter estimation.
A model has been formulated to calculate the protein and amino acid requirements for growing turkeys. A requirement was taken as the sum of the needs for maintenance, for gain in carcass weight without feathers, and for the feathers. Parameters used in the model were obtained from a study of carcass composition along the growth period and from two balance trials with adult turkeys. The maintenance requirement was considered to be the sum of the needs for replacing skin and intestinal protein losses and for the obligatory creatinine excretion in the urine. Nitrogen retention was determined at 2.3 g/day in protein-fed adult (17 kg) male turkeys as an estimate for the replacement needs for skin protein loss. Creatinine excretion in these birds was 123 mg/day. When fed an N-free diet, 16.5 kg adult turkeys excreted a total of 3.5 g/day amino acids as a result of intestinal losses. The protein requirement for maintenance was estimated at 32 mg/day/g2/3 of body weight by summing up the different needs and dividing by .85, the coefficient of protein absorption. Protein and amino acid analyses of nude carcass and feathers and the growth curve were used to calculate the requirements for growth. These were then related to energy intake predicted by equations, which included previously determined coefficients for maintenance and growth (Hurwitz et al., 1980a), and the results of carcass lipid analysis. The calculated amino acid requirements were similar to those of the National Research Council (1977). | {
"pile_set_name": "PubMed Abstracts"
} |
Communication deficits in children undergoing temporal lobectomy.
To examine formal thought disorder and discourse (cohesive) devices that make speech coherent prospectively in seven children, aged 5.7 to 16.7 years, before and after temporal lobectomy for intractable cut points determined from sensitivity and specificity analyses of formal thought disorder and discourse measures in 22 children with complex partial seizure disorder and 45 normal children. Before surgery, the mean illogical thinking and discourse scores of the surgical candidates were in the pathological range. After a mean postoperative follow-up period of 15.1 months, their illogical thinking (but not their discourse scores) decreased significantly to the normal range. These preliminary findings are discussed in terms of the possible role of postsurgical changes in seizure control, behavior, antiepileptic drugs, cognition, and prefrontal function. | {
"pile_set_name": "PubMed Abstracts"
} |
[Autoimmune hemolytic anemia followed by acute granulocytic leukemia (author's transl)].
The authors report a case of acute myeloid leukemia developing 5 years after the onset of an autoimmune hemolytic anemia. The cytological origin of the proliferation is difficult to assess: the presence of prominent membranous complexes and a strong positivity of acid phosphatase reaction favour its megacaryocytic origin. However the cytological evolution pleads for the development of successive clones of monocytic origin, may be modified by the treatment. Are the two diseases successive consequences of preexisting state of immunodepression or was such a state created by the autoimmune anemia? | {
"pile_set_name": "PubMed Abstracts"
} |
Patients with pulse rate changes in irritable bowel syndrome. Further evidence of altered autonomic function.
Patients with the irritable bowel syndrome have significantly more rapid and significantly greater slowing of the pulse rate than do age-, sex-, and stressor-matched controls when their pulse rate is measured at initial outpatient clinic attendance. These findings provide further evidence that altered autonomic function exists in patients with the irritable bowel syndrome. | {
"pile_set_name": "PubMed Abstracts"
} |
Technique and indications of the forearm flap in hand surgery. A report of thirty-three cases.
The Chinese forearm flap is vascularized by the radial artery and may be used as an island or a free flap. Used as an island flap, the pitfalls of the microsurgical procedures required for a free flap, may be avoided. Vascular anastomosis is easily performed owing to the large diameter of the vessel. The possibility of using it as a composite flap with fascia, tendons, muscle, bone or nerve makes this flap a "must" in reconstructive surgery of the hand. A series of 33 cases was reviewed in an attempt to summarize their indications and contraindications respectively. | {
"pile_set_name": "PubMed Abstracts"
} |
Linezolid treatment of glycopeptide-resistant Enterococcus faecium in very low birth weight premature neonates.
Glycopeptide-resistant Enterococcus faecium has emerged as an important nosocomial pathogen with limited therapeutic options. Here we report the successful treatment of glycopeptide-resistant E. faecium infection in two very low birth weight premature infants with the new oxazolidinone linezolid. Treatment with linezolid at a dosage of 10 mg/kg every 8 h intravenously for a duration of 16 days and 14 days, respectively, was well tolerated and led to complete clinical recovery and clearance of the organism from all body sites. The two cases support the clinical efficacy and safety of linezolid in very low birth weight premature neonates with glycopeptide-resistant E. faecium infections. | {
"pile_set_name": "PubMed Abstracts"
} |
Transient period of correlated bursting activity during development of the mammalian retina.
The refinement of early connections in the visual pathway requires electrical activity in the retina before the onset of vision. Using a multielectrode array, we have shown that the spontaneous activity of cells in the neonatal ferret retina is correlated by patterns of periodically generated traveling waves. Here, we examine developmental changes in the characteristics of the waves and show that retinal ganglion cells participate in these patterns of activity, which are seen during the same period as synaptic modification in the lateral geniculate nucleus; that the waves subside gradually as the connectivity in the lateral geniculate nucleus stabilizes; and that their spatial structure allows for refinement of the retinotopic map, as well as for eye-specific segregation in the lateral geniculate nucleus. | {
"pile_set_name": "PubMed Abstracts"
} |
[Renal cell carcinoma extending into the right atrium. Case report].
We report the clinical case of a 48-year-old woman with advanced renal cell carcinoma, and an inferior vena cava thrombus extending into the right atrium. The patient underwent complete tumor excision with radical nephrectomy and inferior vena cava trombectomy using adjunctive cardiopulmonary bypass and deep hypothermic circulatory arrest. Pathological studies revealed no capsular invasion by the renal cell carcinoma. Eighteen months postoperatively the patient is asymptomatic and on immunosuppressive therapy for suspected metastatic disease. We discuss the morbilidity, mortality and long term survival of patients with similar presentation of renal cell carcinoma based in a review of the published literature. Long term survival after surgical treatment is possible in a patient with localized renal cell carcinoma extending into the right atrium. In patient with localized renal cell carcinoma and inferior vena cava tumor thrombus the cephalad extent of inferior vena caval involvement does not appear to influence the prognosis. | {
"pile_set_name": "PubMed Abstracts"
} |
A bactericidal activity of microsomal preparations.
Exposure of suspension of Salmonella typhimurium tester strains TA1535, TA1537 and TA98 to mouse hepatic post-mitochondrial supernatants (S9) resulted in appreciable loss of bacterial viability. This lethal activity was destroyed by heating at 56 degrees C for 30 min. The toxic component of the S9 has not been identified. | {
"pile_set_name": "PubMed Abstracts"
} |
The effects of complications and comorbidities on the quality of preventive diabetes care: a literature review.
Although concurrent conditions such as complications and comorbidities are common in people with diabetes, both are often omitted from studies of the quality of diabetes preventive care. This systematic review of the literature on the quality of diabetes preventive care assesses not only trends in the reporting of and adjusting for complications and comorbidities, but also the limitations of current measures of complications and comorbidities. This review identified 34 studies in which the quality of diabetes preventive care was assessed with process measures and complications or comorbidities were reported. More often than not, the studies identified the presence of certain complications or comorbidities, counted complications or comorbidities, or used comorbidity indices to measure morbidity. While earlier studies reported the prevalence of complications or comorbidities, more recent studies use complications or comorbidities as covariates in regression models. Despite this progress, the effects of complications and comorbidities on care processes are unclear because of cross-study variation among measures of complications and comorbidities and because very few studies address the independent effects of complications and comorbidities. Effective measures of complications and comorbidity are necessary to evaluate the quality of diabetes preventive care, particularly for patients with concurrent conditions. Current reported measures of complications and comorbidities may not address constructs related to quality, underscoring the need for a methodology that is better than the approaches now documented in the literature. | {
"pile_set_name": "PubMed Abstracts"
} |
Solvent effects in hydrogen abstraction from cholesterol by benzophenone triplet excited state.
Hydrogen abstraction from the C-7 position of cholesterol (Ch) by triplet excited benzophenone (BZP) exhibits remarkable solvent-dependence in product studies. Kinetic measurements on the intramolecular version of the process in dyads containing covalently linked Ch and BZP units reveal important solvent effects and significant stereodifferentiation. | {
"pile_set_name": "PubMed Abstracts"
} |
High-resolution fMRI: overcoming the signal-to-noise problem.
Increasing the spatial resolution in functional Magnetic Resonance Imaging (fMRI) inherently lowers the signal-to-noise ratio (SNR). In order to still detect functionally significant activations in high-resolution images, spatial smoothing of the data is required. However, conventional non-adaptive smoothing comes with a reduced effective resolution, foiling the benefit of the higher acquisition resolution. We show how our recently proposed structural adaptive smoothing procedure for functional MRI data can improve signal detection of high-resolution fMRI experiments regardless of the lower SNR. The procedure is evaluated on human visual and sensory-motor mapping experiments. In these applications, the higher resolution could be fully utilized and high-resolution experiments were outperforming normal resolution experiments by means of both statistical significance and information content. | {
"pile_set_name": "PubMed Abstracts"
} |
Dihydroflavonol BB-1, an extract of natural plant Blumea balsamifera, abrogates TRAIL resistance in leukemia cells.
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis in many transformed cells but not in normal cells and, hence, has emerged as a novel anticancer agent. Previously, we showed that although most adult T-cell leukemia/lymphoma (ATLL) cells express the TRAIL death receptor DR4 (TRAIL-R1) or DR5 (TRAIL-R2), they are resistant to TRAIL. Thus, in this study, we tried to find natural products that can overcome TRAIL resistance. Among more than 150 materials screened, a dihydroflavonol that was extracted from Blumea balsamifera (BB-1) exhibited the most striking synergism with TRAIL. Treatment of the TRAIL-resistant ATLL cell line KOB, with a combination of BB-1 and TRAIL, resulted in apparent apoptosis that was not observed on treatment with either agent alone. Furthermore, pretreatment with BB-1 followed by TRAIL further augmented the synergism. BB-1 increased the level of TRAIL-R2 promoter activity and surface protein expression in a p53-independent manner. TRAIL-R2 siRNA inhibited the synergism, indicating that sensitization was caused by the increase of TRAIL-R2 expression. More interestingly, similar effects were observed in other leukemia cell lines by exactly the same mechanisms. These results suggest that combined treatment with BB-1 and TRAIL may be a new strategy for cancer therapy. | {
"pile_set_name": "PubMed Abstracts"
} |
[Intramedullary femoral osteosynthesis with the "rendezvous procedure". A new philosophy of retrograde intramedullary nailing].
The disadvantages of retrograde intramedullary nailing are the necessity of explanting the nail through the knee and the occasional difficulties with proximal interlocking. The new retrograde intramedullary nail includes the following innovations: The nail is inserted into the medullary canal beyond the great trochanter, so that a proximal aiming device can be used. The nail is removed through the proximal opening. The distal opening can be closed with the osteochondral cylinder that has been taken out before. In all, 27 patients have been included in this prospective study. The intraoperative data of the implantation has been recorded. A clinical/radiologic examination took place on the day of discharge and 6-12-26 weeks after the operation in order to record the postoperative complications. The mean time of follow-up examinations at the moment is 8.9 months (2-14). No infections have occurred. The healing of the fractures was without any problems. No patient complained of any major problems concerning the knee and the hip. The new Sinart-Nail seems to be a nail that can be implanted easily and without complications. It combines the advantages of the retrograde intramedullary nailing with the advantages of the anterograde explantation. The two aiming devices facilitate a distal and proximate fixation without fluoroscopy. The replanting of the osteochondral cylinder reduces internal damage to the knee. | {
"pile_set_name": "PubMed Abstracts"
} |
Stability limits for visual feedback posturography in vestibular rehabilitation.
Vestibular rehabilitation therapy (VRT) is used to enhance compensation after peripheral vestibular lesion. In visual feedback posturography (VFP) a patient can practice postural control actively on a force platform and progress in the VRT can be objectively measured. The aim of the study was to determine normative values for stability limits (SL) in the VFP. Eleven healthy subjects were measured with force platform in visual feedback condition. Subjects were instructed to move their center of gravity (COG) marker to given targets, which were displayed on a computer screen in front of them. Target distance from the center of the platform was increased step by step to determine the SL in 8 directions. Hit delays (HD) to the targets and hold percentages (HP) within the targets were measured to quantify the difficulty of the individual targets as a function of subject's shoe size. With fixed feet position the SL in anterior direction increased 1.2 times the absolute increase in shoe length. An almost similar increase was found in anterolateral direction. The knowledge of the mechanical SL is important, because targets near SL should be avoided for safety reasons. | {
"pile_set_name": "PubMed Abstracts"
} |
Abiotic synthesis of organic compounds under the conditions of submarine hydrothermal systems: a perspective.
Deep-sea hydrothermal systems have been proposed to be likely environments for chemical evolution and the origin of life on Earth. Recently, experiments have, therefore, been carried out in order to test the hypothesis that amino acids can be synthesized under conditions representing hydrothermally altered oceanic crust. The variety of amino acids that have been detected in such experiments corresponds roughly to that reported previously for electric sparking in reducing gas mixtures. The relative yields of the protein amino acids detected are significantly higher than in electric spark discharge experiments, and the overall yields are about an order of magnitude higher. The amino acids are all racemic. | {
"pile_set_name": "PubMed Abstracts"
} |
[A case of very-long-chain acyl-CoA dehydrogenase deficiency with adolescent onset being diagnosed by immunostain of biopsy muscle].
We report a case of myopathic form of very-long-chain acyl-CoA dehydrogenase (VLCAD) deficiency with adolescent onset which presented with recurrent rhabdomyolysis and was diagnosed by immunostain of biopsy muscle. She was an 18-year-old woman who showed recurrent episodes of rhabdomyolysis after exercise since the age of 15. The diagnosis was made by the immunostain using anti-VLCAD antibody and the measurement of acyl-CoA dehydrogenase activity for the biopsy muscle. Her elder sister had also showed recurrent episodes of rhabdomyolysis at least two times. The analysis of genomic DNA on blood samples of the patient and her sister was performed and the same mutations were identified. Hence, these sister were revealed to have VLCAD deficiency. We should keep in mind this disorder for those presenting with recurrent rhabdomyolysis. In addition, as far as we know, this is the first report that a correct diagnosis was obtained by immunostain. Immunostain is probably a useful diagnostic procedure to identify an uncommon myopathy. | {
"pile_set_name": "PubMed Abstracts"
} |
Sudden cardiac death.
This article uses a biologic model of sudden cardiac death to identify structural abnormalities that serve as the substrate for sustained arrhythmias and functional changes that are transient and necessary for triggering an arrhythmia. The biologic framework is a valuable tool to use in understanding the relationship between structure and function and in organizing patients' clinical presentations and outcomes. | {
"pile_set_name": "PubMed Abstracts"
} |
Tranexamic Acid: Promise or Panacea: The Impact of Air Medical Administration of Tranexamic Acid on Morbidity, Mortality, and Length of Stay.
The MATTERs and CRASH-2 studies demonstrate that tranexamic acid (TXA) reduces mortality in patients with traumatic hemorrhage. However, their results, conducted in foreign countries and with U.S. military soldiers, provoke concerns over generalizability to civilian trauma patients in the United States was reported. The evaluation of patient outcomes following treatment with TXA by a civilian air medical program. A retrospective chart review of trauma patients transported by air service to a Level 1 trauma center was conducted. For the purposes of intervention evaluation, patients meeting this criterion for the 2 years (2012-2014) prior to therapy implementation were compared with patients treated during the 2-year study period (2014-2016). Goals were to evaluate morbidity, mortality, and length of stay. During the review, 82 control and 49 study patients were identified as meeting inclusion criteria. Patients in the control group were found to be less acute, which correlated with shorter hospital stays and better discharge outcomes. Multiple patients in the study group who should have expired according to a significantly elevated Trauma Revised Injury Severity Score (TRISS) survived, whereas multiple patients in the control group expired despite a low TRISS calculation. This is the first outcome-based study conducted in a U.S. trauma system. The outcomes in civilian trauma patients in the United States do not follow that of the previous MATTERs and CRASH-2 studies. However, this study still shows benefit to TXA administration and reduced risk for administration to patients with head trauma and occurrence of venous thromboembolism. Randomized control trials are needed to evaluate the role of TXA administration in the United States. | {
"pile_set_name": "PubMed Abstracts"
} |
A novel heat shock protein 90 inhibitor potently targets adrenocortical carcinoma tumor suppression.
Adrenocortical carcinoma is an aggressive cancer with a poor prognosis. Long noncoding RNAs are differentially expressed in cancer patients and contribute to cellular homeostasis, survival, and metastasis. We hypothesize that our novel C-terminal Hsp90 inhibitor KU758 can effectively target adrenocortical carcinoma cells and favorably alter long noncoding RNA expression. Cell viability after KU758 treatment was measured in the adrenocortical carcinoma cell lines SW13, RL251, and NCI-H295R by MTS assay. Cellular mobility and metastatic potential after Hsp90 inhibition was measured through migration, invasion, and aggregate formation assays. β-catenin activity in NCI-H295R cells was determined by immunofluorescence and polymerase chain reaction. Long noncoding RNA expression was determined by polymerase chain reaction array after Hsp90 inhibition. KU758 is selective for adrenocortical carcinoma cells with IC50 values of 0.6 to 2.4 μM. KU758 treatment can effectively reduce migration, invasion, and aggregate formation in NCI-H295R and SW13 cells. β-catenin activity is decreased after treatment with KU758. Treatment with KU758 is associated with overall statistically significant upregulation of long noncoding RNA expression, including the tumor suppressor GAS5, which is implicated in the β-catenin and mammalian target of rapamycin pathways in adrenocortical carcinoma. The novel C-terminal Hsp90 inhibitor KU758 is effective in the treatment of adrenocortical carcinoma cells and can significantly alter long noncoding RNA expression for tumor suppression. | {
"pile_set_name": "PubMed Abstracts"
} |
The risk of vascular surgery in a metropolitan community. With observations on surgeon experience and hospital size.
From 1978 through 1981 complete perioperative information concerning a total of 10,189 peripheral vascular procedures performed in northeastern Ohio was recorded in the computer registry of The Cleveland Vascular Society. This report is an analysis of mortality and morbidity rates for all 5686 operations involving carotid endarterectomy (N = 2646), lower extremity revascularization (N = 1987), and abdominal aortic aneurysm resection (N = 1053). The operative mortality rate was 1.2% for carotid reconstruction, 2.8% for femoropopliteal or distal bypass, 3.5% for aortofemoral revascularization, and 11.9% for aortic aneurysm resection (elective operations 6.5%; emergency operations 32.9%). Postoperative strokes occurred after endarterectomy in 2.7% of patients having preoperative neurologic symptoms and in 2.0% of those with asymptomatic carotid stenosis. Lower extremity amputation was unavoidable in 1.5% of patients after aortofemoral reconstruction and in 6.0% after femoropopliteal or distal bypass. Statistical testing indicated that the operative mortality rate was not related to the respective size of the 27 hospitals involved in the survey. The relative annual experience of the 29 participating surgeons significantly influenced only the mortality rate of elective aneurysm resection and the amputation rate after femoropopliteal or distal revascularization. This study suggests that the results of major arterial reconstruction in metropolitan areas may be expected to be comparable to those of published series if the responsible surgeons are specifically trained and maintain an active interest in the field of vascular surgery. | {
"pile_set_name": "PubMed Abstracts"
} |
A cross-cultural study of parental conflict and eating disorders in a non-clinical sample.
Previous British studies have found that EAT scores of second generation British Asian schoolgirls are higher than those of White schoolgirls (Furnham & Husain, 1999; McCourt & Waller, 1995; Mumford et al., 1991), and that these scores are positively associated with parental over-protection (Furnham & Hussain, 1999). This study looked at the relationship between parental conflict and parental overprotection and EAT scores in three cultures. The three groups, all of late adolescent females, were British Caucasians (N=116), immigrant British Asians from Pakistan (N= 118), and Pakistanis tested in Pakistan (N=114). A 22 item conflict questionnaire was constructed and administered to 355 participants, along with the PBI, EAT-26, and Body Shape Belief Scale (BSBS). It was predicted that the British Asians would have higher EAT, parental protection and conflict scores than the other two groups. It was also predicted that EAT scores would be highly correlated with conflict scores. All hypotheses were supported, and over-protection scores were noticeably highest in the British Asian group. They also had a significant amount of more conflict with parents than any of the other cultural groups. EAT scores were associated with conflict and over-protection. Results are discussed in terms of the literature in the field. | {
"pile_set_name": "PubMed Abstracts"
} |
Hindquarter amputation for lymphangiomatosis.
We describe the presentation of a 16-year-old boy with locally advanced lymphangiomatosis affecting the left thigh and groin. This is a difficult disease to both diagnose and treat, and one in which radical surgery may be necessary to effect a cure or even palliation. In this case, hindquarter amputation allowed a previously wheelchair-bound teenager to mobilize unaided. | {
"pile_set_name": "PubMed Abstracts"
} |
Identification of an active sequence within the first immunoglobulin domain of intercellular cell adhesion molecule-1 (ICAM-1) that interacts with fibrinogen.
Monocytic cells bind fibrinogen (fg) through integrin alphaMbeta2. fg-bound monocytic cells demonstrate an enhanced adhesion to endothelial cells, which is dependent on intercellular adhesion molecule-1 (ICAM-1). Our studies differentiate fg interactions with stimulated and resting endothelial cells, which are ICAM-1 dependent and independent, respectively. This report documents a direct interaction between fg and intact ICAM-1 and with a two-Ig domain form of ICAM-1. A small region within the first Ig domain of ICAM-1, ICAM-1-(8-21) (KVILPRGGSVLVTC), was identified to interact with fg in a specific and selective manner. ICAM-1-(8-21) bound to plasmin-derived fg fragments X, D100, and D80 but not to fragment E. Consistent with this finding, fg gamma-chain peptide, fg-gamma-117-133, blocked fg interaction with ICAM-1-(8-2 1. ICAM-1-(8-21) peptide and antibodies directed against ICAM-1-(8-21) also blocked the adhesion and binding of ICAM-1-bearing Raji cells with fg. ICAM-1-(8-21) and fg-gamma-117-133 are likely to be one of the contact pairs mediating fg-ICAM-1 interactions. | {
"pile_set_name": "PubMed Abstracts"
} |
Contemporary potencies of minocycline and tetracycline HCL tested against Gram-positive pathogens: SENTRY Program results using CLSI and EUCAST breakpoint criteria.
Tetracycline class agents vary widely in their activity against emerging important antimicrobial-resistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter spp. Also, published susceptibility breakpoints are discordant between the Clinical and Laboratory Standards Institute (CLSI), the European Committee on Antimicrobial Susceptibility Testing (EUCAST), and regulatory-approved documents. We have assessed the impact of these differences for tetracycline HCL and minocycline when tested against contemporary Gram-positive pathogens. The SENTRY Antimicrobial Surveillance Program (2011) compared minocycline and tetracycline HCL activity via reference methods (M07-A9) using a worldwide collection of S. aureus (SA; 4917 strains with 1955 MRSA), Streptococcus pneumoniae (SPN; 1899), S. pyogenes (GRA; 246), and S. agalactiae (GRB; 217). Regardless of applied categorical breakpoints, minocycline exhibited wider coverage (% susceptible) than tetracycline HCL of 4.5-11.8/0.5-2.6/1.4-2.3/0.4-0.4% for MRSA/SPN/GRB/GRA, respectively. Lower EUCAST susceptible breakpoints produced reduced susceptibility rates for minocycline ranging from no difference (≤0.5 μg/mL) for GRA to -8.9% (≤1 μg/mL) for MRSA (97.2% susceptible by CLSI; 88.3% by EUCAST). Use of tetracycline HCL-susceptible results to predict minocycline susceptibility was very accurate (99.0-100.0%), with absolute categorical agreement rates ranging from 92.1% to 98.4% (CLSI) to 98.4% to 99.6% (EUCAST) for streptococci; greatest predictive error was noted using the CLSI breakpoints (14.7%) compared to EUCAST criteria (only 5.0%; acceptable), both for MRSA testing dominated by false-resistant results for minocycline. In conclusion, minocycline demonstrates continued superior in vitro activity compared to tetracycline HCL when testing SA (especially MRSA) and pathogenic streptococci. When testing tetracyclines, laboratories must recognize the expanded spectrum of minocycline against certain pathogens and utilize methods minimizing interpretive error. We conclude that EUCAST breakpoint criteria (≤0.5 or ≤1 μg/mL) represent the most conservative (better recognize strains with tet resistance mechanisms) and accurate tetracycline breakpoint guidelines for testing contemporary isolates of Gram-positive cocci. | {
"pile_set_name": "PubMed Abstracts"
} |
Multiple giant superior mesenteric artery branch aneurysms.
Mesenteric arterial branch aneurysms are rare. Giant, multiple, mesenteric branch artery aneurysms are even more uncommon, and only a few reports exist in the literature. We describe a 73-year-old asymptomatic female found to have an abdominal bruit and subsequently diagnosed with multiple mesenteric branch artery aneurysms by computed tomography and angiography. In addition, the patient was found to have celiac artery occlusion at its origin. Risk factors include hypertension, hyperlipidemia, grandmultiparity, and tobacco dependence. She was treated successfully with open surgery including ligation of multiple branch aneurysms and vein bypass reconstruction to preserve hepatic and mesenteric artery flow. Our purpose is to review this uncommon entity in terms of diagnosis, etiology, treatment options, and literature review. | {
"pile_set_name": "PubMed Abstracts"
} |
Virus isolate from carp: genetic characterization reveals a novel picornavirus with two aphthovirus 2A-like sequences.
Picornaviruses have been isolated from a variety of hosts, mainly mammals and birds. Here, we describe the sequence analysis of carp picornavirus 1 (CPV-1) F37/06 that was isolated from an organ pool (heart, brain, liver) of a common carp (Cyprinus carpio). This carp perished after an accidental discharge of liquid manure into a fish pond and presented without obvious clinical symptoms. Experimental intraperitoneal infection of young carp with CPV-1 revealed no clinical signs, but the virus was re-isolated from various organs. Sequence analysis of almost the complete genome (7632 nt excluding the poly-A tract) revealed a novel picornavirus clade. In phylogenetic trees, the polymerase sequence clusters with parechoviruses, duck hepatitis A virus, eel picornavirus and aquamavirus A. The ORF includes 6807 nt and encodes a polyprotein of 2269 amino acids. CPV-1 has a genome layout like that of picornaviruses except for the presence of two aphthovirus 2A-like NPGP sequence motifs: VPg+5'UTR[1AB-1C-1D-2A1(npgp)/2A2(npgp)-2B-2C(ATPase)/3A-3B(VPg)-3C(pro)-3D(pol)]3'UTR-poly-A. 2A1(npgp) and 2A2(npgp) are separated by 133 amino acids. The proteins 2A2(npgp), 2B, 3A and 3B(VPg) have no significant similarity to the corresponding proteins of other picornaviruses. Amino acid identities of the orthologous proteins P1, 2C, 3C(pro) and 3D(pol) range from 16.4 to 40.8 % in the eel picornavirus/CPV-1 comparison. 3D(pol) shows the closest similarity to eel picornavirus, with an amino acid identity of 40.8 %, followed by human parechovirus (36.5 %), duck hepatitis A virus (32.7 %) and swine pasivirus (29.3 %). Both the unique genome organization and low sequence similarity support the assignment of CPV-1 to a novel picornavirus species within a novel genus. | {
"pile_set_name": "PubMed Abstracts"
} |
FNA diagnosis of retroperitoneal leiomyosarcoma metastasizing to the breast.
Fine-needle aspiration (FNA) biopsy is one of the first-line investigations in any breast lump, and hence cytomorphological recognition of nonmammary metastatic tumors to the breast and their distinction from primary tumors is important. Breast metastasis from extra-mammary malignancy is rare, constituting 2% of breast tumors; even rarer are metastatic leiomyosarcomas. Our patient presented with a breast lump 2 years after operative removal of a retroperitoneal leiomyosarcoma. The breast lump was confirmed to be a metastasis from the earlier primary. Herein, we report the first case of a retroperitoneal leiomyosarcoma metastatic to the breast diagnosed by Fine Needle Aspiration. | {
"pile_set_name": "PubMed Abstracts"
} |
The development of visual acuity in the Mongolian gerbil (Meriones unguiculatus).
The development of visual acuity for vertically oriented gratings was followed in gerbil pups from 34 days of age until adulthood using a jumping stand procedure. Acuity was found to improve gradually from an initial level of about 0.7 cycles/degree to asymptotic values of 1.2-1.6 cycles/degree by 70 days of age. Acuity did not vary as a function of viewing distance over the range tested (17.5-46 cm). Practice was found to play some role in the acuity changes seen but cannot alone explain the results. The contributions of other performance variables and of neural development are considered. | {
"pile_set_name": "PubMed Abstracts"
} |
Charge Transfer, Complexes Formation and Furan Fragmentation Induced by Collisions with Low-Energy Helium Cations.
The present work focuses on unraveling the collisional processes leading to the fragmentation of the gas-phase furan molecules under the He+ and He2+ cations impact in the energy range 5-2000 eV. The presence of different mechanisms was identified by the analysis of the optical fragmentation spectra measured using the collision-induced emission spectroscopy (CIES) in conjunction with the ab initio calculations. The measurements of the fragmentation spectra of furan were performed at the different kinetic energies of both cations. In consequence, several excited products were identified by their luminescence. Among them, the emission of helium atoms excited to the 1s4d 1D2, 3D1,2,3 states was recorded. The structure of the furan molecule lacks an He atom. Therefore, observation of its emission lines is spectroscopic evidence of an impact reaction occurring via relocation of the electronic charge between interacting entities. Moreover, the recorded spectra revealed significant variations of relative band intensities of the products along with the change of the projectile charge and its velocity. In particular, at lower velocities of He+, the relative cross-sections of dissociation products have prominent resonance-like maxima. In order to elucidate the experimental results, the calculations have been performed by using a high level of quantum chemistry methods. The calculations showed that in both impact systems two collisional processes preceded fragmentation. The first one is an electron transfer from furan molecules to cations that leads to the neutralization and further excitation of the cations. The second mechanism starts from the formation of the He-C4H4O+/2+ temporary clusters before decomposition, and it is responsible for the appearance of the narrow resonances in the relative cross-section curves. | {
"pile_set_name": "PubMed Abstracts"
} |
Angiotensin-(1-7) reduces the perfusion pressure response to angiotensin II and methoxamine via an endothelial nitric oxide-mediated pathway in cirrhotic rat liver.
Recent studies have shown that, in cirrhosis, portal angiotensin-(1-7) [Ang-(1-7)] levels are increased and hepatic expression of angiotensin converting enzyme 2 (ACE2) and the Mas receptor are upregulated, but the effects of Ang-(1-7) on hepatic hemodynamics in cirrhosis have not been studied. This study investigated the effects of Ang-(1-7) on vasoconstrictor-induced perfusion pressure increases in cirrhotic rat livers. Ang II or the alpha 1 agonist methoxamine (MTX) were injected in the presence or absence of Ang-(1-7), and the perfusion pressure response was recorded. Denudation of vascular endothelial cells with sodium deoxycholate was used to investigate the contribution of endothelium to the effects of Ang-(1-7). Ang-(1-7) alone had no effect on perfusion pressure. However, it reduced the maximal vasoconstriction response and area under the pressure response curve to Ang II and MTX by >50% (P < 0.05). This effect of Ang-(1-7) was not blocked by Mas receptor inhibition with A779 or by Ang II type 1 and type 2 receptor and bradykinin B(2) receptor blockade and was not reproduced by the Mas receptor agonist AVE0991. D-Pro(7)-Ang-(1-7), a novel Ang-(1-7) receptor antagonist, completely abolished the vasodilatory effects of Ang-(1-7), as did inhibition of endothelial nitric oxide synthase (eNOS) with N(G)-nitro-L-arginine methyl-ester, guanylate cyclase blockade with ODQ and endothelium denudation. The functional inhibition by D-Pro(7)-Ang-(1-7) was accompanied by significant (P < 0.05) inhibition of eNOS phosphorylation. This study shows that Ang-(1-7) significantly inhibits intrahepatic vasoconstriction in response to key mediators of increased vascular and sinusoidal tone in cirrhosis via a receptor population present on the vascular endothelium that is sensitive to D-Pro(7)-Ang-(1-7) and causes activation of eNOS and guanylate cyclase-dependent NO signaling pathways. | {
"pile_set_name": "PubMed Abstracts"
} |
Prevalence and characterization of lamivudine-resistant hepatitis B virus mutations in HIV-HBV co-infected individuals.
To determine the prevalence of hepatitis B virus (HBV) genotypic resistance to lamivudine, identify risk factors associated with lamivudine resistance, and characterize the pattern of HBV polymerase mutations in patients co-infected with HIV. Retrospective cross-sectional study. Thirty-three chronic HBV-infected patients were identified from a cohort of 1719 HIV-infected individuals. Patient information was collected from case records, HBV DNA was measured on stored serum by polymerase chain reaction, and positive samples underwent sequencing of HBV polymerase, basal core promoter and precore regions. Three groups of patients were identified: group 1 were viraemic in the absence of lamivudine-resistance mutations, group 2 were viraemic in association with lamivudine-resistance mutations, and group 3 were not viraemic. Group 2 patients with lamivudine-resistant mutations had significantly higher HBV-DNA viral loads but did not differ in duration of lamivudine therapy, HBV genotype, HIV viral load or CD4 cell count compared with patients with wild-type HBV. Group 2 individuals also demonstrated significantly higher serum alanine aminotransferase (ALT) levels than group 1, who were higher than group 3. Unique mutations were detected in HBV polymerase, including rtV173L plus rtL180M plus rtM204V, which occurred in three patients. This virus has the in-vitro characteristics of a 'vaccine escape' mutant of HBV. Genotypic HBV lamivudine resistance was found in 39% of HIV-HBV co-infected individuals treated with lamivudine as part of highly active antiretroviral therapy. These patients exhibited significantly elevated HBV viral loads and serum ALT, and three were infected with a lamivudine-resistant HBV strain that was potentially transmissible to HBV-vaccinated individuals. | {
"pile_set_name": "PubMed Abstracts"
} |
Assessment of the efficiency and economic viability of various methods of treatment of sanitary landfill leachate.
This study assesses the efficiency of various physico-chemical, biological and other tertiary methods for treating leachate. An evaluation study on the treatability of the leachate from methane phase bed (MPB) reactor indicated that at an optimum hydraulic retention time of 6 days, the efficiency of the reactor in terms of biological oxygen demand (BOD) and chemical oxygen demand (COD) removal was 91.29 and 82.69%, respectively. Recycling of the treated leachate through the municipal solid waste layers in the leachate recycling unit (LRU) resulted in a significant increase in the biodegradation of organics present in the leachate. Optimum BOD and COD removal efficiencies were achieved at the third recycle; additional recycling of the leachate did not produce any significant improvement. Physico-chemical treatment of the leachate demonstrated that alum and lime (Option 2) were more economical than coagulants lime and MgCO(3). A cost analysis of the economics of the various treatments revealed that the alternative treatment consisting of a MPB bed followed by a LRU and aerated lagoon is the most cost-effective treatment. However, the alternative consisting of a MPB followed by the LRU and a soil column, which is slightly more costly, would be the most appropriate treatment when adequate land is readily available. | {
"pile_set_name": "PubMed Abstracts"
} |
Patent ductus arteriosus in an infant with atrioventricular septal defect and pulmonary hypertension: diagnosis by transesophageal color flow echocardiography.
An infant with complete atrioventricular septal defect (atrioventricular canal) was examined by standard transthoracic two-dimensional pulsed Doppler and color Doppler echocardiography. No evidence of ductus arteriosus was present. Preoperative transesophageal echocardiography identified a patent ductus: the left-to-right shunt seen by color Doppler echocardiography was enhanced by pharmacologic maneuvers aimed at decreasing pulmonary vascular resistance and increasing systemic vascular resistance. Transesophageal echocardiography was found to be more sensitive than transthoracic echocardiography even in a small infant. | {
"pile_set_name": "PubMed Abstracts"
} |
Ondansetron attenuates depression co-morbid with obesity in obese mice subjected to chronic unpredictable mild stress; an approach using behavioral battery tests.
The aim of the present work was to investigate the role of ondansetron on the high fat diet (HFD) induced obese mice for behavioral and biochemical alterations using chronic unpredictable mild stress (CUMS) model of depression. Animals were fed with high fat diet for 14 weeks and subjected to different stress procedures for 4 weeks. Treatment with ondansetron was started on day 15. After day 28 behavioral assays and biochemical estimations were performed. Behavioral paradigms viz. sucrose preference test, locomotor score, forced swim test (FST) and elevated plus maze (EPM), whereas biochemical parameters like plasma glucose, total cholesterol, triglycerides and total proteins were estimated. Results examines that in behavioral assays, ondansetron significantly (P < 0.05) increased sucrose consumption, reduced immobility time in FST, increased the percent entries and time in open arm in EPM. In biochemical assessments elevated plasma glucose, total cholesterol, triglycerides and total proteins were significantly (P < 0.05) reversed by ondansetron treatment in HFD obese animals subjected to CUMS. The study indicates that the obese mice subjected to CUMS exhibited severe depressive-like symptoms and ondansetron significantly reversed the behavioral and biochemical alterations. In the present study the plasma glucose level indicates that, it could be "altered glucose level" playing an important role in depression co-morbid with obesity. Ondansetron through allosteric modulation of serotonergic system elevates the serotonin level and thereby regulates the insulin secretion and hence, reversing the "altered glucose level", could be the possible antidepressive-like mechanism against depression co-morbid with obesity. | {
"pile_set_name": "PubMed Abstracts"
} |
Prevention of duodenal ulcer recurrence with penicillin. A double-blind, placebo-controlled trial.
Eradication of Helicobacter pylori is associated with a reduced recurrence of duodenal ulcer (DU). The relationship between H. pylori and DU has been interpreted as causal, but the evidence has been criticized for methodologic reasons. To ascertain whether an antibiotic with no effect on epithelial-cell integrity prevents DU recurrence, we conducted a randomized double-blind trial of phenoxymethylpenicillin (PEN), 2.4 twice daily, and placebo (PLA). Patients with an active DU and positive H. pylori culture from antral biopsy specimens were treated with 40 mg omeprazole daily for 4 weeks, but at week 2 they were allocated at random to PEN (85 patients) or PLA (85 patients) for up to 14 weeks. Those without recurrence during this treatment were followed up for another 6 months. Endoscopy and H. pylori culture were performed at the end of the treatment period and at the end of follow-up, and in between if ulcer symptoms recurred. During the treatment period the ulcer relapse rate was 5 of 58 (9%) in the PEN group and 34 of 68 (50%) in the PLA group (P < 0.0001, log-rank test), with 53% and 14%, respectively, of the patients in the two groups being H. pylori-negative. The relapse rate in the PEN group did not differ between H. pylori-negative and H. pylori-positive patients. The recurrence rate in the PEN group remained low for another 5 months but then approached the rate in the PLA group. The prevalence of H. pylori-negative patients at the end of follow-up was 20% in the PEN group and 10% in the PLA group. These data provide strong evidence that DU has a bacterial cause, with H. pylori as the likely agent. | {
"pile_set_name": "PubMed Abstracts"
} |
Efficacy and safety of endoscopic sleeve gastroplasty for obesity patients: a meta-analysis.
Endoscopic sleeve gastroplasty (ESG) is a minimally invasive, effective, and safe technique for weight loss intervention. Since a relatively small number of cases were present in previous studies, this study aimed to elucidate the efficacy and safety of ESG. Relevant publications were identified through searching PubMed, EMBASE, Cochrane, and Web of Science before March 1, 2019. The percentage of total body weight loss (%TBWL), percentage of excess weight loss (%EWL), and the adverse event rate in each follow-up session were extracted, pooled, and analyzed. Forest plots were graphed based on random effects models. A total of 1542 patients from nine studies were eligible for analysis. The pooled results of %TBWL at 1, 3, 6, and 12 months were 8.78% (p = 0.000), 11.85% (p = 0.000), 14.47% (p = 0.024), and 16.09% (p = 0.063), respectively. The pooled results of %EWL at 1, 3, 6, and 12 months were 31.16% (p = 0.000), 43.61% (p = 0.000), 53.14% (p = 0.000), and 59.08% (p = 0.015), respectively. Finally, the pooled rate of mild adverse events was 72% (p < 0.01), and the pooled estimate of severe adverse events was only 1% (p = 0.08). Although the conventional surgical sleeve gastrectomy is the gold standard for bariatric surgery, ESG could be a promising minimally invasive alternative for treating obesity with satisfactory efficacy and low risk. | {
"pile_set_name": "PubMed Abstracts"
} |
Fiber type composition of unoperated rat soleus and extensor digitorum longus muscles after unilateral isotransplantation of a foreign muscle in long-term experiments.
We examined the effects of the unilateral heterochronous isotransplantation on the fiber type composition and myosin heavy chain (MyHC) isoform content of unoperated slow soleus and fast extensor digitorum longus muscles of female inbred Lewis strain rats. Comparison was made between "control" unoperated muscles of experimental rats (after intramuscular transplantation surgery) with the corresponding muscles of completely naive (unoperated) rats of three age groups (5-, 8- and 14-month-old). This was done in order to ascertain whether these muscles can be used as reliable controls to the transplanted and host muscles for our ongoing grafting experiments. The fiber type composition was determined by assessing the histochemical reaction for myofibrillar adenosine triphosphatase, the MyHC isoform content was determined immunocytochemically using monoclonal antibodies specific to different MyHC isoforms and by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Our experiments show that the heterochronous intramuscular isotransplantation procedure had no significant effect on the fiber type composition and MyHC isoform content of the "control" unoperated muscles of the experimental rats when compared to the corresponding muscles of the naive animals. Furthermore, the duration and type of isotransplantation did not also lead to differences among corresponding "control" muscles of experimental animals. We conclude that the unoperated muscles of the experimental rats can be used as controls in our current transplantation project dealing with long-term grafting experiments. | {
"pile_set_name": "PubMed Abstracts"
} |
High-throughput microfluidic device for single cell analysis using multiple integrated soft lithographic pumps.
The ability to accurately control fluid transport in microfluidic devices is key for developing high-throughput methods for single cell analysis. Making small, reproducible changes to flow rates, however, to optimize lysis and injection using pumps external to the microfluidic device are challenging and time-consuming. To improve the throughput and increase the number of cells analyzed, we have integrated previously reported micropumps into a microfluidic device that can increase the cell analysis rate to ∼1000 cells/h and operate for over an hour continuously. In order to increase the flow rates sufficiently to handle cells at a higher throughput, three sets of pumps were multiplexed. These pumps are simple, low-cost, durable, easy to fabricate, and biocompatible. They provide precise control of the flow rate up to 9.2 nL/s. These devices were used to automatically transport, lyse, and electrophoretically separate T-Lymphocyte cells loaded with Oregon green and 6-carboxyfluorescein. Peak overlap statistics predicted the number of fully resolved single-cell electropherograms seen. In addition, there was no change in the average fluorescent dye peak areas indicating that the cells remained intact and the dyes did not leak out of the cells over the 1 h analysis time. The cell lysate peak area distribution followed that expected of an asynchronous steady-state population of immortalized cells. | {
"pile_set_name": "PubMed Abstracts"
} |
[Effect of self-foot reflexology massage on depression, stress responses and immune functions of middle aged women].
This study was aimed to identify the effects of a self-foot reflexology massage on depression, stress responses and functions of the immune system of middle-aged women. This study was a one group pretest-posttest experimental design and the data was collected from August 1st, 2004 to May 31st, 2005. The subjects consisted of 46 middle-aged women (40 - 64 years) who were recruited from the Community Health Center in Busan city. Subjects were not treated for 4 weeks, subsequently they were trained in self foot reflexology massage for 2 weeks, and then they did their own daily for 6 weeks (2 days at the research center, 5 days at home). The outcome variables were measured 4 times, at baseline, pre training, after training, and after the intervention. The collected data was analyzed using repeated measure ANOVA by the SPSS/WIN program. There was a statistically significant difference in depression, perceived stress, systolic blood pressure, natural-killer cells and Ig G. However, there was not a statistically significant difference in diastolic blood pressure, pulse or serum cortisol. These results suggest that a self-foot reflexology massage could be utilized as an effective nursing intervention to reduce depression and stress responses, and to strengthen immune systems in middle-aged women. | {
"pile_set_name": "PubMed Abstracts"
} |
Reflections on the socio-economic correlates of health.
Income, education, occupation, age, sex, marital status, and ethnicity are all correlated with health in one context or another. This paper reflects on the difficulties encountered in deriving robust scientific conclusions from these correlations or drawing reliable policy applications. Interactions among the variables, nonlinearities, casual inference, and possible mechanisms of action are discussed. Strategies for future work are suggested, and researchers are urged to pay special attention to possible interactions among health, genes, and socio-economic variables. | {
"pile_set_name": "PubMed Abstracts"
} |
Natural history, epidemiology and screening of unruptured intracranial aneurysms.
The prevalence of intracranial aneurysms is 2.3% (95% CI, 1.7-3.1%); most of these aneurysms are small and located in the anterior circulation. Risk factors are age, female gender, smoking, hypertension, excessive use of alcohol, having one or more affected relatives with SAH and autosomal dominant polycystic kidney disease. Most studies on risk of rupture have methodological weaknesses; an important flaw is that observed risks are recalculated to yearly risks of rupture, assuming a constant risk of growth and rupture of aneurysms. In reality, it is much more likely that aneurysms have long periods of low risk and short periods of high risk of growth and rupture. The overall risk of rupture found in follow-up studies is around 1% per year. Size is the most important risk factor for rupture, with smaller risks for smaller aneurysms. Other risk factors are the site of the aneurysm (higher risk for posterior circulation aneurysms), age, female gender, population (higher risks in Finland and Japan) and, probably also, smoking. There are no good comparisons between clipping and coiling of unruptured aneurysms. Both treatment modalities have a risk of around 6% of complications leading to death or dependence of help for activities of daily living for aneurysms smaller than 10mm. These risks increase with larger size of aneurysms. For clipping, the risk seems to increase with age, for coiling this is less apparent. The efficacy of coiling on the long term is unsettled. In deciding whether or not to treat an aneurysm, life expectancy is a pivotal factor; other important factors are the size and the site of the aneurysm. If the aneurysm is left untreated, follow-up imaging may be considered to detect growth of aneurysms, but the frequency and effectiveness of repeated imaging are unknown. | {
"pile_set_name": "PubMed Abstracts"
} |
High-grade pancreatic intraepithelial lesions: prevalence and implications in pancreatic neoplasia.
High-grade pancreatic intraepithelial neoplasia (PanIN-3), a precursor of pancreatic ductal adenocarcinoma (PDAC), is not universally detected in resected pancreatic neoplasms. We sought to determine the prevalence and prognostic relevance of PanIN-3 lesions in primary surgical resections of PDACs and intraductal papillary mucinous neoplasms (IPMNs). A retrospective review of a tertiary care center pathology database (1/2000-6/2014) was performed. Demographics, imaging, pathology, disease-recurrence, and survival data were reviewed. A total of 458 patients who underwent primary pancreatic resection were included. "PanIN-3" lesions were found in 74 (16.2%) patients who either had PDAC (n=67) or main duct (MD)-IPMN (n=7). Among IPMN-MDs, PanIN-3 lesions were exclusively found in those with pathological evidence of chronic pancreatitis. For PDACs, the median overall survival (OS) for pancreata with PanIN-3 lesions was significantly better than those without (OS 1.12 years, inter-quartile range [IQR] 0.72, 2.05 years vs OS 0.86 years, IQR 0.64, 1.60 years respectively; P=0.04). Multivariate Cox regression analysis demonstrated that the presence of PanIN-3 lesions was associated with a reduced risk of death (HR=0.43; 95% CI: 0.23-0.82; P=0.01). Following primary resection of pancreatic adenocarcinoma, the lower survival observed in patients without PanIN-3 lesions might suggest a state of complete or accelerated transformation. Further investigations are necessary to validate these findings that might impact disease prognosis and management. | {
"pile_set_name": "PubMed Abstracts"
} |
Work happiness among teachers: a day reconstruction study on the role of self-concordance.
Self-concordant work motivation arises from one's authentic choices, personal values, and interests. In the present study, we investigated whether self-concordant motivation may fluctuate from one work-related task to the next. On the basis of self-determination theory, we hypothesized that momentary self-concordance buffers the negative impact of momentary work demands on momentary happiness. We developed a modified version of the day reconstruction method to investigate self-concordance, work demands, and happiness during specific work-related tasks on a within-person and within-day level. In total, 132 teachers completed a daily diary on three consecutive work days as well as a background questionnaire. The daily diary resulted in 792 reported work activities and activity-related work demands, self-concordance, and happiness scores. Multilevel analysis showed that-for most work activities-state self-concordant motivation buffered the negative association of work demands with happiness. These findings add to the literature on motivation and well-being by showing that the levels of self-concordance and happiness experienced by employees vary significantly on a within-day level and show a predictable pattern. We discuss theoretical and practical implications of the findings to increase employees' well-being. | {
"pile_set_name": "PubMed Abstracts"
} |
Load dependency and reliability of microhardness measurements on acid-etched enamel surfaces.
The load dependency of hardness measurements was investigated on sound and demineralized enamel samples. For all samples investigated, the Knoop hardness number (KHN) varied as a function of the applied load. Higher loads enhanced indenter penetration into the test surface. The variation in KHN values observed in demineralized enamel, both surface-softened and surface-etched, was explained by a decrease in porosity as a function of depth. The reading error for indentations made on surface enamel etched with 37% phosphoric acid gel for 30 s was found to be slightly elevated compared to indentations made on a polished sound enamel surface. It was concluded that the surface microhardness technique can be used for the longitudinal investigation of etched surface enamel. | {
"pile_set_name": "PubMed Abstracts"
} |
Involvement of noradrenergic and corticoid receptors in the consolidation of the lasting anxiogenic effects of predator stress.
The roles of beta-NER (beta-noradrenergic receptor), GR (glucocorticoid) and mineral corticoid receptors (MR) in the consolidation of anxiogenic effects of predator stress were studied. One minute after predator stress, different groups of rats were injected (ip) with vehicle, propranolol (beta-NER blocker, 5 and 10 mg/kg), mifepristone (RU486, GR blocker, 20 mg/kg), spironolactone (MR blocker, 50 mg/kg), propranolol (5 mg/kg) plus RU486 (20 mg/kg) or the anxiolytic, chloradiazepoxide (CPZ, 10 mg/kg). One week later, rodent anxiety was assessed in elevated plus maze, hole board, light/dark box, social interaction and acoustic startle. Considering all tests except startle, propranolol dose dependently blocked consolidation of lasting anxiogenic effects of predator stress in all tests. GR receptor block alone was ineffective. However, GR block in combination with an ineffective dose of propranolol did blocked consolidation of predator stress effects in all tests, suggesting a synergism between beta-NER and GR. Surprisingly, MR block prevented consolidation of anxiogenic effects in all tests except the light/dark box. CPZ post stress was ineffective against the anxiogenic impact of predator stress. Study of startle was complicated by the fact that anxiogenic effects of stress on startle amplitude manifested as both an increase and a decrease in startle amplitude. Suppression of startle occurred in stressed plus vehicle injected groups handled three times prior to predator stress. In contrast, stressed plus vehicle rats handled five times prior to predator stress showed increases in startle, as did all predator stressed only groups. Mechanisms of consolidation of the different startle responses appear to differ. CPZ post stress blocked startle suppression but not enhancement of startle. Propranolol post stress had no effect on either suppression or enhancement of startle. GR block alone post stress prevented suppression of startle, but not enhancement. In contrast blocking GR and beta-NER together prevented startle enhancement. MR block also prevented startle enhancement. Effects of MR block on startle suppression were not tested. Delay of habituation to startle was found in all stressed rats. Consolidation of delay of habituation was blocked or attenuated by post stress MR block, GR plus beta-NER block and CPZ but not by post stress GR or beta-NER block alone. Taken together, present findings suggest consolidation of lasting anxiogenic effects of predator stress may share some of the same neurochemical mechanisms implicated in some forms of fear memory consolidation. Implications of these findings for the study of stress-induced changes in affect including posttraumatic stress disorder (PTSD) are discussed. | {
"pile_set_name": "PubMed Abstracts"
} |
Right ventricular rupture during closed-chest cardiopulmonary resuscitation after pneumonectomy with pericardiotomy: a case report.
The collapse of a patient immediately after right pneumonectomy with right pericardiotomy resulted in closed-chest cardiopulmonary resuscitation, subsequent thoracotomy, and demise secondary to right ventricular rupture. Closed-chest resuscitation with opened and closed chest tubes and medical and fluid interventions were inadequate, necessitating subsequent thoracotomy. Right ventricular rupture during resuscitation was found during subsequent thoracotomy. This rupture and inadequacy of closed-chest resuscitation were felt to be associated with the operative pneumonectomy and pericardiotomy. Pathophysiology and the role of open-heart vs. closed-chest resuscitative measures are discussed. | {
"pile_set_name": "PubMed Abstracts"
} |
Achieving improvements across the HIV treatment cascade: a clinical management algorithm based on IAPAC's entry into and retention in care and antiretroviral therapy adherence guidelines.
Engagement in care is directly linked to achieving HIV viral suppression, yet over the past 2 years analyses of data related to the US HIV epidemic have revealed that many people living with HIV/AIDS are failing to successfully navigate the HIV treatment cascade, with only between an estimated 19% and 28% achieving the ultimate goal of antiretroviral therapy (ART): viral suppression. We propose a clinical management algorithm that captures key operational interventions recommended in recently published guidelines on entry into and retention in care and ART adherence. | {
"pile_set_name": "PubMed Abstracts"
} |
The organization of the rat GRP78 gene and A23187-induced expression of fusion gene products targeted intracellularly.
Expression of the glucose-regulated protein, GRP78, is markedly increased when cells are placed in a variety of stressful environments (i.e., low glucose medium, calcium ionophore treatment). In this report, the genomic organization of the rat GRP78 gene is described. This gene comprises eight exons and encodes a protein which is highly hydrophilic with the notable exception of several short hydrophobic domains. The first hydrophobic region, 18 amino acids at the N-terminus of the protein, putatively acts as a signal sequence to target GRP78 into the endoplasmic reticulum (ER). By ligating portions of the GRP78 gene and its promoter to the bacterial gene encoding chloramphenicol acetyltransferase (CAT), we created heterologous CAT genes inducible by calcium ionophore A23187. Through immunofluorescence analysis, the intracellular localizations of endogenous GRP78 and fusion CAT proteins under normal growth and A23187-induced conditions are identified. By fusing the GRP78 signal sequence to CAT, we influence intracellular targeting of the CAT protein into the ER. | {
"pile_set_name": "PubMed Abstracts"
} |
Suspected primary hypoparathyroidism in a domestic ferret (Mustela putorius furo).
A 4-year-old castrated male domestic ferret (Mustela putorius furo) was examined because of a 3-week history of intermittent seizures, signs of depression, hypocalcemia, and hyperphosphatemia. Plasma biochemical analysis confirmed hyperphosphatemia (17.7 mg/dL) and low concentrations of total (4.3 mg/dL) and ionized (0.49 mmol/L) calcium. Serum parathyroid hormone concentration (2.30 pmol/L) was low or in the low part of the reference interval. Calcium gluconate was administered (2.0 mg/kg/h [0.9 mg/lb/h], IV), followed by a transition to administration of calcium carbonate (53 mg/kg [24.1 mg/lb], PO, q 12 h) and dihydrotachysterol (0.02 mg/kg/d [0.009 mg/lb/d], PO). Attitude of the ferret improved and seizures ceased as blood calcium concentrations increased. The ferret was reexamined because of seizures approximately 1 year after oral maintenance administration of dihydrotachysterol and calcium was initiated. The ferret responded well to emergency and long-term treatment but then was lost to follow-up monitoring. The ferret died approximately 2 years after the initial evaluation and treatment. Hypertrophic cardiomyopathy was diagnosed during necropsy, but the parathyroid glands could not be identified. To the authors' knowledge, primary hypoparathyroidism has not previously been reported in a ferret. The condition should be considered for ferrets with hypocalcemia and hyperphosphatemia without azotemia. Treatment with dihydrotachysterol and oral supplementation of calcium appeared to be a viable option for long-term management. | {
"pile_set_name": "PubMed Abstracts"
} |
Remediation of sandy soils contaminated with hydrocarbons and halogenated hydrocarbons by soil vapour extraction.
This paper presents the study of the remediation of sandy soils containing six of the most common contaminants (benzene, toluene, ethylbenzene, xylene, trichloroethylene and perchloroethylene) using soil vapour extraction (SVE). The influence of soil water content on the process efficiency was evaluated considering the soil type and the contaminant. For artificially contaminated soils with negligible clay contents and natural organic matter it was concluded that: (i) all the remediation processes presented efficiencies above 92%; (ii) an increase of the soil water content led to a more time-consuming remediation; (iii) longer remediation periods were observed for contaminants with lower vapour pressures and lower water solubilities due to mass transfer limitations. Based on these results an easy and relatively fast procedure was developed for the prediction of the remediation times of real soils; 83% of the remediation times were predicted with relative deviations below 14%. | {
"pile_set_name": "PubMed Abstracts"
} |
Intensified daunorubicin in induction therapy and autologous peripheral blood stem cell transplantation in postremission therapy (Double-7 protocol) for adult acute myeloid leukemia.
To investigate whether an intensified dose of daunorubicin (DNR) in induction therapy and autologous peripheral blood stem cell transplantation (PBSCT) in the postremission period are effective treatments, we used a Double-7 protocol to treat adult patients with de novo acute myeloid leukemia (excluding M0 and M3). Induction therapy consisted of 40 mg/m2 of DNR intravenous drip infusion for 7 days and 200 mg/m2 of ara-C by continuous infusion for 7 days (7 + 7 DC regimen). Patients who achieved complete remission (CR) were given high-dose chemotherapy with autologous PBSCT in postremission therapy. Of the 22 assessable patients, 16 attained CR (73%). Disease-free survival (DFS) and overall survival (OS) at 3 years were 61.2% and 48.1%, respectively. Nine of the CR patients underwent PBSCT without therapy-related mortality. Patients in a favorable cytogenetic group (n = 7) attained 100% CR and long-term survival (71.4% DFS and 85.7% OS at 3 years). Thus, intensified DNR administration of 280 mg/m2 (40 mg/m2 per day for 7 days) in induction therapy for adult patients younger than 60 years of age might be optimal or at least comparable with the new anthracyclines such as idarubicin. In addition, autologous PBSCT in postremission therapy might improve DFS and OS, at least for patients in a favorable cytogenetic group, such as those with a t(8;21) abnormality. | {
"pile_set_name": "PubMed Abstracts"
} |
Ruminants express a prolactin receptor of M(r) 33,000-36,000 in the mammary gland throughout pregnancy and lactation.
Developmental variation in the expression of the prolactin receptor in the ruminant mammary gland was investigated. Affinity chromatography revealed that bovine prolactin and human GH each bound to the same mammary gland proteins, yielding fractions enriched in binding activity and a protein of M(r) 36,000, assumed to be a bovine prolactin receptor. Affinity cross-linking of 125I-labelled human GH to mammary microsomes confirmed that the M(r) 36,000 protein was a bovine prolactin receptor. Binding assays of receptors in microsomes from the mammary tissue of cows and ewes at various stages of the lactational/reproductive cycle indicated developmental regulation of receptor concentration, but not receptor type, as no other bovine prolactin receptor type was detected by affinity cross-linking. These results suggest that differences in the response to prolactin in the mammary gland at various developmental stages in ruminants are not due to the expression of different forms of the prolactin receptor, and the lack of a prolactin effect on established lactation in ruminants is not due to the absence of the M(r) 36,000 form of the prolactin receptor. | {
"pile_set_name": "PubMed Abstracts"
} |
Intraepidermal IgA pustulosis preceding a CD30+ anaplastic large T-cell lymphoma.
We report the case of a 62-year-old patient with an extracutaneous CD30+ anaplastic large T-cell lymphoma which was preceded by intraepidermal IgA pustulosis (IEAP). The clinical picture was identical to Sneddon-Wilkinson disease and histologic examination showed subcorneal pustules with slight acantholysis. Direct immunofluorescence exclusively revealed IgAK deposits on the plasma membrane of suprabasal keratinocytes. Indirect immunofluorescence and immunoelectrophoresis showed no evidence of monoclonal IgA gammopathy. IEAP responded to dapsone treatment (100 mg/day). Two months after disappearance of active cutaneous lesions, the patient developed generalized lymphadenopathy due to CD30+ anaplastic T-cell lymphoma. | {
"pile_set_name": "PubMed Abstracts"
} |
The HOX genes network in uro-genital cancers: mechanisms and potential therapeutic implications.
Genito-urinary malignancies (prostate, bladder, renal and testicular cancers) rank high among human tumors with an incidence that varies with age and organ involvement. Prostate cancer is the most commonly detected male cancer followed by bladder and kidney cancers, less frequent in women. Testicular cancer, although rare, is the most frequent cancer in males under 35. The majority of oncogenic and tumor suppressor signaling pathways involved with urogenital cancers converge on sets of transcription factors that ultimately control gene expression resulting in tumor formation and metastatic progression. The activity of these transcription factors is modulated by multiple mechanisms spanning from transcriptional regulation, deregulation of the splicing, maturation, export and location of mRNAs, protein synthesis and post-translational modifications. The recent involvement of the epigenitic mechanisms in the generation and the evolution of cancer has produced a great deal of interest. This is related to the possibility that revealing these mechanisms able to regulate the cell memory program (the gene systems polycomb, trithorax and HOX) may generate important biological and therapeutic achievements. The HOX gene network is the only physically and functionally identifiable transcription factor network located in the human genome controlling crucial cellular processes. Here we describe the implication of the HOX genes in the urogenital embryonic development and cancers. We further highlight the mechanisms uncovered along these processes and involving the HOX genes. Finally, we foresee the specific targeting of HOX genes and in general the cell memory gene program in the therapeutic setting of urogenital malignancies due to their upstream location in these stepwise cell processes and their early deregulation in cancer evolution. | {
"pile_set_name": "PubMed Abstracts"
} |
[Culture optimization for protein expression in Escherichia coli].
We developed a new culture-complex auto-inducing media (CAI) for heterogenous protein expression in Escherichia coli. To test expression efficiency in the CAI, we constructed seven different plasmids named p-1, p-2, p-3, p-4, p-5, p-6 and p-7. These plasmids were transformed into E. coli BL21, then expressed in both Luria-Bertani madia LB and CAI. To improve the expression level even more, we analyzed the composition of the CAI and optimized the culture. The expression levels of seven fusion proteins in CAI were four times higher than those in Luria-Bertani. Through a series of changes we formed a new optimized culture (CAI-4). Comparing to the expression levels of these fusion proteins (P-1, P-2, P-3) in CAI, the expression levels of fusion proteins in CAI-4 increased 2-fold. | {
"pile_set_name": "PubMed Abstracts"
} |
Polyanion inhibitors of HIV and other viruses. 7. Polyanionic compounds and polyzwitterionic compounds derived from cyclodextrins as inhibitors of HIV transmission.
New polyanionic compounds were obtained from radical addition of thiomalic acid and mercaptopropionic acid onto perallylated cyclodextrins (CDs) under UV irradiation with a catalytic amount of alpha,alpha'-azobis(isobutyronitrile). All these polyanions, bearing 18-48 carboxylate groups, inhibited human immunodeficiency virus type 1 (HIV-1) strain IIIB replication in MT-4 cells at a 50% inhibitory concentration (IC50) of 0.1-2.9 microM, while not being toxic to the host cells at concentrations up to 62 microM. These compounds were also active against a clinical HIV-1 isolate (HE) at >/=4-fold higher concentrations. Only some compounds showed activity against the two HIV-2 strains (ROD and EHO) but at higher concentrations than those required to inhibit HIV-1 (IIIB and HE) replication. In addition, these compounds were not active against the M-tropic HIV-1 strain BaL but were active against simian immunodeficiency virus [SIV (MAC251)]. These compounds were also inhibitory to the replication of human cytomegalovirus at an IC50 of 1-10 microM, but not herpes simplex virus (type 1 and type 2) or other (picorna-, toga-, reo-, orthomyxo-, paramyxo-, bunya-, rhabdo-, and poxvirus) viruses. Radical addition on perallylated CDs of a protected cysteine gave polyzwitterionic compounds. None of these last compounds proved inhibitory to the replication of HIV-1, HIV-2, or any of the other viruses tested. | {
"pile_set_name": "PubMed Abstracts"
} |
Access to and use of non-inpatient services in New York State among racial-ethnic groups.
OBJECTIVE Nationwide studies contrasting service use of racial-ethnic groups provide an overview of disparities, but because of variation in populations and service systems, local studies are required to identify specific targets for remedial action. The authors report on the use of non-inpatient services regulated in New York State (NYS) and report use by the state's larger cultural groups. METHODS Data from the NYS Patient Characteristics Survey were used to estimate annual treated prevalence and treatment intensity, defined as the average number of annual weeks in service for non-Hispanic blacks, Hispanics, Asians, and non-Hispanic whites. The latter rates were obtained for specific types of treatment use, by person's age and diagnosis, for the state and for population density-defined regions. Statistical methods contrasted rates of whites with other groups. RESULTS A total of 578,496 individuals in these racial-ethnic groups were served in 2,500 programs, and 51% of those served were nonwhite. Treated prevalence rates of whites were lower than those of blacks and Hispanics and were substantially higher than prevalence rates for Asians. Statewide treatment intensity rates of all racial-ethnic and age groups were comparable except for lower use among Asians >65. Key findings from granular analyses were lower treatment intensity rates for black youths with disruptive disorders, Hispanic adults with anxiety disorders, and Asians >65 with depression compared with white counterparts. In upstate metropolitan areas, black youths and Hispanic adults received services in fewer weeks than whites, and in the New York City metropolitan area, whites >65 had higher treatment intensity rates than contrast groups. CONCLUSIONS Findings suggest a need for assistance to black families in negotiating the multiple systems used by their children, clinical training focusing on cultural symptom presentation, screening of Asians in community settings, and mandated cultural competency assessments for all programs. | {
"pile_set_name": "PubMed Abstracts"
} |
Alternatives to warfarin--the next generation of anticoagulants.
Anticoagulation therapy plays an important role in the management of cardiovascular disease. Currently, oral anticoagulation therapy is reliant on vitamin K antagonists (VKA). In clinical practice, VKA present several limitations including a narrow therapeutic window and frequent drug and food interactions. Despite the clear clinical need for alternative anticoagulants it is only within the last decade that significant progress has been made. These new anticoagulants target specific factors in the hemostatic network and appear to overcome some of the difficulties seen with VKA. Many have now progressed to phase III clinical trials including patients with cardiovascular disease. This review aims to highlight the exciting progress that has been made in the development of these new anticoagulants. It will focus on the key agents that have demonstrated the most promise in clinical trials to date. This will include data on cardiovascular indications for anticoagulant therapy. Finally, the review aims to analyze the future prospects for these new agents. Several issues remain to be addressed for these agents to finally replace vitamin K antagonists as the mainstay of anticoagulant therapy. | {
"pile_set_name": "PubMed Abstracts"
} |
Testing spectrophotometer linearity.
It is shown that the linearity of the photometric scale of spectrophotometers is not self-evident. The widely used Lambert-Beer test does not give a conclusive proof of linearity. Light addition methods do not show this disadvantage. In the form of the supplementary light method, as used here, they are suited for testing commercial instruments. The arrangement for testing the Zeiss DMR 21 recording spectrophotometer is shown, and the results do not reveal a nonlinearity exceeding 0.1% T. The accuracy of the method is limited only by the precision of the instrument under test. | {
"pile_set_name": "PubMed Abstracts"
} |
Nephrogenic metaplasia of the ureter.
We present what, to our knowledge, is the first reported case of nephrogenic metaplasia of the ureter. Nephrogenic metaplasia involves the transitional epithelium of the urinary tract and results in the formation of epithelial tubules, which are histologically similar to renal tubules. Special stains demonstrated both intracellular and intraluminal mucin. Ultrastructurally, the lesion consisted of epithelial cells with sparse microvilli and a thick basal lamina. The criteria for diagnosis and differentiation from carcinoma are discussed. | {
"pile_set_name": "PubMed Abstracts"
} |
[An improved method using k-means to determine the optimal number of clusters, considering the relations between several variables].
In this article, we propose a non-hierarchical clustering method that can consider the relations between several variables and determine the optimal number of clusters. By utilizing the Mahalanobis distance instead of the Euclidean distance, which is calculated in k-means, we could consider the relations between several variables and obtain better groupings. Assuming that the data are samples from a mixture normal distribution, we could also calculate Akaike's information criterion (AIC) and the Bayesian information criterion (BIC) to determine the number of clusters. We used simulation and real data examples to confirm the usefulness of the proposed method. This method allows determination of the optimal number of clusters, considering the relations between several variables. | {
"pile_set_name": "PubMed Abstracts"
} |
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