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Assessment of the potential for maladaptive parenting in expectant fathers with the Adult-Adolescent Parenting Inventory (AAPI).
The purpose of this investigation was to determine if expectant fathers could be assessed for potential maladaptive parenting with the Adult-Adolescent Parenting Inventory (AAPI). Image factor analysis with matrix rotation by SPSSx was used to replicate Bavolek's (1984) earlier work. It was concluded that the AAPI was appropriate for use with expectant fathers because their responses were not significantly different than those of other populations. The 232 subjects' scores indicated that although they had appropriate attitudes toward empathy and family roles, they had limited knowledge of child growth and development and had strong beliefs in physical discipline. Recommendations include the need for further studies using the AAPI to validate its effectiveness as a predictive tool in the primary prevention of child abuse and neglect. | {
"pile_set_name": "PubMed Abstracts"
} |
[The S.I.G.N.A.L.-Intervention Project to combat violence against women].
Domestic violence has profound effects on the health of women. According to the World Health Organization, violence is a significant risk factor for morbidity and mortality in women. If violence is not taken into account as a cause of health problems, there is a high risk of inappropriate health care. More attention to the issue of domestic violence in medical history, diagnosis and therapy is the objective of the "S.I.G.N.A.L. Intervention Project to Combat Violence Against Women". The "S.I.G.N.A.L. Project" is the first intervention project against violence in a medical setting in Germany. It was started in 1999 in the emergency room of Benjamin Franklin University Hospital (Charite Campus Benjamin Franklin). The goal of the project is to initiate prevention and intervention for violence against women by providing the abused women with appropriate support and treatment. The program is based on the intervention objectives: ask about abuse, assess for danger, inform and refer victims to counselling programs and women's shelters, and document injuries and health problems for use in legal proceedings. | {
"pile_set_name": "PubMed Abstracts"
} |
Clinical use of biologic response modifiers in cancer treatment: an overview. Part I. The interferons.
Interferons are proteins with antiviral, antiproliferative, and immune-regulating activity. They are classified as alfa, beta, or gamma on the basis of antigenicity and biologic properties. Alfa interferons as single-agent therapy produce clinical improvement in approximately 90 percent of patients with hairy-cell leukemia, and up to 70 percent of patients with chronic myelogenous leukemia (CML) in early-stage disease. Prolonged suppression or elimination of the leukemic cell clone by interferon may ultimately increase survival of patients with CML. Interferon is not effective single-agent therapy for multiple myeloma, but improves response rate when combined with conventional agents. AIDS-associated Kaposi's sarcoma demonstrates a 40 percent objective response rate to interferon, with less risk of immune system suppression than conventional cytotoxics. Other applications of alfa interferon include malignant melanoma and renal cell carcinoma. Beta interferon is similar to the alfa subtype and may have utility in treatment of brain tumors. Gamma interferon is an important immune regulator with qualitative and quantitative differences in its efficacy and toxicity when compared with alfa interferon. | {
"pile_set_name": "PubMed Abstracts"
} |
Anticipating the unknown: The stimulus-preceding negativity is enhanced by uncertain threat.
Anticipating the future is an important psychological process that facilitates adaptive functioning. The stimulus-preceding negativity (SPN) is an event-related potential index of anticipation that is elicited in the several hundred milliseconds before an outcome occurs. The SPN is elicited by emotional stimuli, such as electric shocks, and has been proposed to reflect emotional anticipation. To better characterize the functional significance of the SPN, the current study examined the effects of uncertain threat on SPN amplitude. Fifty-three participants from the community completed a task that compared the SPN when anticipating uncertain threat (a 50% chance of electric shock), certain threat (a 100% chance of electric shock), and safety (a 0% chance of electric shock). The SPN was enhanced by uncertain threat relative to certain threat and safety. Thus, the SPN appears to be specifically sensitive to uncertainty above and beyond threat. These results replicate and extend previous findings and inform theoretical accounts regarding the functional significance of the SPN. Furthermore, the findings highlight the utility of the SPN as a measure of anticipation and a tool for research on the effects of uncertainty, in line with recent calls for the development of additional laboratory-based measures of responding to uncertainty. | {
"pile_set_name": "PubMed Abstracts"
} |
Evaluation of Kodak EDR2 film for dose verification of intensity modulated radiation therapy delivered by a static multileaf collimator.
A new type of radiographic film, Kodak EDR2 film, was evaluated for dose verification of intensity modulated radiation therapy (IMRT) delivered by a static multileaf collimator (SMLC). A sensitometric curve of EDR2 film irradiated by a 6 MV x-ray beam was compared with that of Kodak X-OMAT V (XV) film. The effects of field size, depth and dose rate on the sensitometric curve were also studied. It is found that EDR2 film is much less sensitive than XV film. In high-energy x-ray beams, the double hit process is the dominant mechanism that renders the grains on EDR2 films developable. As a result, in the dose range that is commonly used for film dosimetry for IMRT and conventional external beam therapy, the sensitometric curves of EDR2 films cannot be approximated as a linear function, OD = c * D. Within experimental uncertainty, the film sensitivity does not depend on the dose rate (50 vs 300 MU/min) or dose per pulse (from 1.0 x 10(-4) to 4.21 x 10(-4) Gy/pulse). Field sizes and depths (up to field size of 10 x 10 cm2 and depth = 10 cm) have little effect on the sensitometric curves. Percent depth doses (PDDs) for both 6 and 23 MV x rays were measured with both EDR2 and XV films and compared with ion chamber data. Film data are within 2.5% of the ion chamber results. Dose profiles measured with EDR2 film are consistent with those measured with an ion chamber. Examples of measured IMRT isodose distributions versus calculated isodoses are presented. We have used EDR2 films for verification of all IMRT patients treated by SMLC in our clinic. In most cases, with EDR2 film, actual clinical daily fraction doses can be used for verification of composite isodose distributions of SMLC-based IMRT. | {
"pile_set_name": "PubMed Abstracts"
} |
The prevalence of hepatitis C virus antibodies in thalassemic patients in the south of Thailand.
One hundred and one thalassemic patients, 37 with homozygous beta-thalassemia, 60 with beta-thalassemia Hb E and 4 with hemoglobin H disease with Hb Constant Spring were studied. Twenty-four of 101 (23.8%) tested positive for antibody to hepatitis C virus (anti-HCV). Anti-HCV positivity among those with homozygous beta-thalassemia was significantly higher than anti-HCV positivity among the beta-thalassemic Hb E group. The number of blood transfusions received by anti-HCV positive thalassemic patients was significantly higher than that by anti-HCV negative thalassemic patients. Ninety per cent of anti-HCV positive thalassemic patients had persistently or intermittently raised SGPT levels. | {
"pile_set_name": "PubMed Abstracts"
} |
Stem cell based therapy for skeletal muscle diseases.
The use of stem cells to repair and replace damaged skeletal muscle cells in chronic, debilitating muscle diseases such as the muscular dystrophies holds great promise. Different stem cell populations, both of embryonic and adult origin display the potential to generate skeletal muscle cells and have been studied in animal models of muscular dystrophy. These include muscle derived satellite cells; bone marrow derived mesenchymal stem cells, muscle or bone marrow side population cells, circulating CD133+ cells and cells derived from blood vessel walls such as mesoangioblasts or pericytes. The design of effective stem cell based therapies requires a detailed understanding of the molecules and signaling pathways which determine myogenic lineage commitment and differentiation. We discuss the great strides that have been made in delineating these pathways and how a better understanding of muscle stem cell biology has the potential to lead to more effective stem cell based therapies for skeletal muscle regeneration for devastating muscle diseases. | {
"pile_set_name": "PubMed Abstracts"
} |
Discovery and Biochemical Characterization of the UDP-Xylose Biosynthesis Pathway in Sphaerobacter thermophilus.
The biosynthesis of UDP-xylose requires the stepwise oxidation/ decarboxylation of UDP-glucose, which is catalyzed by the enzymes UDPglucuronic acid dehydrogenase (UGD) and UDP-xylose synthase (UXS). UDPxylose biosynthesis is ubiquitous in animals and plants. However, only a few UGD and UXS isoforms of bacterial origin have thus far been biochemically characterized. Sphaerobacter thermophilus DSM 20745 is a bacterium isolated from heated sewage sludge, and therefore can be a valuable source of thermostable enzymes of biotechnological interest. However, no biochemical characterizations of any S. thermophilus enzymes have yet been reported. Herein, we describe the cloning and characterization of putative UGD (StUGD) and UXS (StUXS) isoforms from this organism. HPLC- and plate reader-based activity tests of the recombinantly expressed StUGD and StUXS showed that they are indeed active enzymes. Both StUGD and StUXS showed a temperature optimum of 70°C, and a reasonable thermal stability up to 60°C. No metal ions were required for enzymatic activities. StUGD had a higher pH optimum than StUXS. The simple purification procedures and the thermotolerance of StUGD and StUXS make them valuable biocatalysts for the synthesis of UDP-glucuronic acid and UDP-xylose at elevated temperatures. The biosynthetic potential of StUGD was further exemplified in a coupled enzymatic reaction with an UDP-glucuronosyltransferase, allowing the glucuronylation of the natural model substrate bilirubin. | {
"pile_set_name": "PubMed Abstracts"
} |
G protein coupled receptor interactions with cholesterol deep in the membrane.
G protein coupled receptors (GPCRs) are located in membranes rich in cholesterol. The membrane spanning surfaces of GPCRs contain exposed backbone carbonyl groups and residue side chains potentially capable of forming hydrogen bonds to cholesterol molecules buried deep within the hydrophobic core of the lipid bilayer. Coarse-grained molecular dynamics (CGMD) simulations allow the observation of GPCRs in cholesterol-containing lipid bilayers for long times (50μs), sufficient to ensure equilibration of the system. We have detected a number of deep cholesterol binding sites on β2 adrenergic and A2A adenosine receptors, and shown changes in these sites on agonist binding. The requirements for binding are modest, just a potential hydrogen bond partner close to a cleft or hole in the surface. This makes it likely that similar binding sites for cholesterol will exist on other classes of membrane protein. | {
"pile_set_name": "PubMed Abstracts"
} |
[Tocolysis with beta sympathomimetics alone or combined with the calcium antagonist verapamil? Influence of verapamil on the fetus].
In a retrospective study, patients who underwent tocolytic therapy by beta-mimetics were investigated. 308 received Fenoterol monotherapy (group A) and 163 a combination of Fenoterol with the calcium-antagonist Verapamil in a dosage-relation of 1/20 (group B). The review of 287 cardiotocograms showed a significant increase in the foetal heart rate in both groups by about 13% after 114 minutes (A) and 146 minutes (B). Although the diaplacental transfer of Fenoterol to the foetus is very small, a high percentage of foetuses showed an increase in heart rate (84.7% in A, 86% in B). There was a significantly longer duration of foetal tachycardia in group B. Both groups were not different in respect of perinatal mortality (5.4% in A, 5.2% in B), birth weight, APGAR score, and the reasons for paediatric care. The great difference in placental permeability for Fenoterol and Verapamil may cause a danger for the foetus. Since no reasonable improvements are seen, additional Verapamil therapy is not recommended. | {
"pile_set_name": "PubMed Abstracts"
} |
Enzymatic synthesis of an indole diterpene by an oxidosqualene cyclase: mechanistic, biosynthetic, and phylogenetic implications.
Petromindole (1) is an unusual indole diterpene that bears a triterpene-like carbon skeleton, suggesting biogenesis from 3-(omega-oxido-geranylgeranyl)indole (4). We found that lupeol synthase (LUP1) from Arabidopsis thaliana cyclizes 4 to 1. Chiral HPLC comparisons of racemic 1 (from biomimetic cyclization of N-pivaloyl-4) with the LUP1 product and authentic 1 established the absolute stereochemistry of petromindole (3S) as that of cyclic triterpenes. Quantum mechanical calculations and conformational analysis of intermediates in the cyclization of 4 to 1 indicated that petromindole biosynthesis differs fundamentally from that of other indole diterpenes. This analysis revealed that radarins also originate from cyclization of 4 but undergo a backbone rearrangement rather than annulation to indole. The combined results support our hypothesis that native fungal petromindole synthase evolved from a pentacyclic triterpene synthase distant from most other indole diterpene synthases. | {
"pile_set_name": "PubMed Abstracts"
} |
Biological Data on Anovia punica Gordon (Coleoptera: Coccinellidae), a Predator of Crypticerya multicicatrices Kondo & Unruh (Hemiptera: Monophlebidae).
The coccinellid beetle Anovia punica Gordon (Coleoptera: Coccinellidae: Noviini) is an important predator of the Colombian fluted scale, Crypticerya multicicatrices Kondo & Unruh (Hemiptera: Monophlebidae). In order to gather information on the biological traits of A. punica, we conducted a series of studies, including of the developmental time of each life history stage, estimation of life table parameters, and predation rates under laboratory conditions [25.1 ± 1.6°C, with 70.5 ± 7.3% RH, and natural light regime, approx. 12:12 (L:D) h]. Developmental stages of A. punica were categorized as follows: egg stage, four larval instars, prepupal instar, pupal instar, and adult. Developmental time from egg to adult emergence averaged 29.41 ± 1.85 days, and 47.6% of the eggs developed to adulthood. Female and male survival was 94.42 and 90 days, respectively. Life table parameters show that one female of A. punica is replaced by 86 females (R 0), the intrinsic growth rate (r m ) was 0.1115, the average generation time (T) was 40 days, and the doubling time (D t ) was 6.2 days. The life table parameters suggest that A. punica can be used as a potential predator of C. multicicatrices and, more importantly, provided baseline information for a mass-rearing protocol. This is the first detailed study on the biology of A. punica that reports the potential of this predator as a biological control agent for scale insects of the tribe Iceryini. | {
"pile_set_name": "PubMed Abstracts"
} |
A variant form of arylsulfatase A in human urine derived directly from the renal pelvis: kinetic and immunological characterization.
Arylsulfatase A (aryl-sulfate sulfohydrolase, EC 3.1.6.1) was examined in voided and in nephrostomic urine. A variant form of the enzyme was found in nephrostomic urine, in addition to the minor form, which is the sole component of arylsulfatase A in voided urine. The nephrostomic enzyme differed from the voided urine enzyme with respect to the kinetic parameters, the isoelectric point, heat stability and immunological reactivity. The isoelectric points of the voided urine and nephrostomic enzymes were 4.7 and 5.3, respectively. The nephrostomic enzyme was more heat-labile at 62.5 degrees C than the voided urine enzyme. Although the Km values of the two enzymes with nitrocatechol sulfate as substrate were almost the same, the V value of the nephrostomic enzyme was approx. one-hundredth that of the voided urine enzyme. The molecular weight (almost 130 000) did not differ between the voided urine and nephrostomic enzymes. It was demonstrated by various methods, using IgG antibody against the purified voided urine enzyme, that the nephrostomic enzyme was antigenically distinct from the voided urine enzyme. | {
"pile_set_name": "PubMed Abstracts"
} |
Controlled Dual Drug Release and In Vitro Cytotoxicity of Electrospun Poly(lactic-co-glycolic acid) Nanofibers Encapsulated with Micelles.
In order to realize controlled dual release of two hydrophobic drugs with distinct rates in a vehicle, novel poly(lactic-co-glycolic acid) (PLGA) composite nanofibers encapsulated with micelles were successfully fabricated by "emulsion-electrospinning." Brefeldin A (BFA) was firstly embedded in monomethoxy-poly(ethylene glycol)-b-poly(L-lactide) (MePEG-PLLA) micelles. By means of "emulsion-electrospinning," paclitaxel (PTX) and polymeric micelles contained BFA were successfully loaded into the electrospun PLGA composite nanofibers. The in vitro release results demonstrated that the location of the drugs in the electrospun fibers determined their release profiles. BFA had a long-term and sustained release while PTX had a relatively rapid release in the dual drugs delivery system. In vitro cytotoxicity studies revealed that the composite nanofibers with two drugs restrained HepG-2 cells more efficiently. These results strongly suggested that the electrospun composite nanofibers containing polymeric micelles can be used as an effective controlled dual release of hydrophobic drugs and were suitable for postoperative chemotherapy of cancers. | {
"pile_set_name": "PubMed Abstracts"
} |
[Proliferation-associated antigens PCNA and Ki-67 in two- and three-dimensional experimental systems of human squamous epithelial carcinomas].
Multiparameter-flow-cytometry was used to compare the levels of two proliferation-associated antigens, proliferating cell nuclear antigen (PCNA) and the Ki-67-related antigen, in squamous carcinoma cells grown as monolayers, multicellular spheroids (MCTS), and xenograft tumors. While the level of Ki-67-positive cells decreased with time of culture, the percentage of PCNA-positive cells stayed high in all experimental states investigated (up to 5 weeks of growth). The reduction of the mean fluorescence of PCNA/cell indicated a different way of regulation of this antigen in squamous carcinoma cells in comparison to other cell types. | {
"pile_set_name": "PubMed Abstracts"
} |
Translucent zirconia in the ceramic scenario for monolithic restorations: A flexural strength and translucency comparison test.
To compare three different compositions of Yttria-Tetragonal Zirconia Polycrystal (Y-TZP) ceramic and a lithium disilicate ceramic in terms of flexural strength and translucency. Three zirconia materials of different composition and translucency, Aadva ST [ST], Aadva EI [EI] and Aadva NT [NT](GC Tech, Leuven, Belgium) were cut with a slow speed diamond saw into beams and tabs in order to obtain, after sintering, dimensions of 1.2×4.0×15.0mm and 15.0×15.0×1.0mm respectively. Blocks of IPS e.max CAD LT were cut and crystallized in the same shapes and dimensions and used as a reference group [LD]. Beams (n=15) were tested in a universal testing machine for three-point bending strength. Critical fracture load was recorded in N, flexural strength (σ in MPa), Weibull modulus (m) and Weibull characteristic strength (σ0 in MPa) were then calculated. Tabs (n=10) were measured with a spectrophotometer equipped with an integrating sphere. Contrast Ratios were calculated as CR=Yb/Yw. SEM of thermally etched samples coupled with lineal line analysis (n=6) was used to measure the tested zirconia grain size. Data were statistically analyzed. Differences in translucency, flexural strength and grain size were found to be statistically significant. CR increased and flexural strength decreased in the following order ST(σ 1215±190MPa, CR 0.74±0.01)>EI(σ 983±182MPa, CR 0.69±0.01)>NT(σ 539±66MPa, CR 0.65±0.01)>LD (σ 377±39Mpa, CR 0.56±0.02). The average grain size was different for the three zirconia samples with NT(558±38nm)>ST(445±34nm)>EI(284±11nm). The zirconia composition heavily influenced both the flexural strength and the translucency. Different percentages of Yittria and Alumina result in new materials with intermediate properties in between the conventional zirconia and lithium disilicate. Clinical indications for Zirconia Aadva NT should be limited up to three-unit span bridges. | {
"pile_set_name": "PubMed Abstracts"
} |
Hepatitis B surface antigen quantification: why and how to use it in 2011 - a core group report.
Quantitative HBsAg had been suggested to be helpful in management of HBV, but assays were cumbersome. The recent availability of commercial quantitative assays has restarted the interest in quantitative serum hepatitis B surface antigen (HBsAg) as a biomarker for prognosis and treatment response in chronic hepatitis B. HBsAg level reflects the transcriptional activity of cccDNA rather than the absolute amount of cccDNA copies. Serum HBsAg level tends to be higher in hepatitis B e antigen (HBeAg)-positive than HBeAg-negative patients. Among patients with a low HBV DNA (<2000IU/ml), HBsAg <1000IU/ml in genotype D HBV infection and HBsAg <100IU/ml in genotype B/C HBV infection is associated with inactive carrier state in HBeAg-negative patients. The HBsAg reduction by nucleos(t)ide analogues (NA) is not as pronounced as by interferon treatment. On peginterferon treatment, sustained responders tend to show greater HBsAg decline than the non-responders. The optimal on-treatment HBsAg cutoff to predict response needs further evaluation in HBeAg-positive patients, but an absence of HBsAg decline together with a <2 log reduction in HBV DNA at week 12 can serve as stopping rule in HBeAg-negative patients with genotype D HBV infection. A rapid serum HBsAg decline during NA therapy may identify patients who will clear HBsAg in the long-term. There are early reports among Asian patients that an HBsAg level of <100IU/ml might predict lower risk of relapse after stopping NA treatment. In clinical practice, serum HBsAg level should be used together with, but not as a substitute for, HBV DNA. | {
"pile_set_name": "PubMed Abstracts"
} |
Applications of microarray technology to pancreatic cancer research and drug development.
As one of the deadliest forms of malignancy in the US and worldwide, pancreatic cancer is becoming a top research priority as therapies at present have had markedly limited or no success in controlling this disease. The arrival and advancement of microarray technology in the last decade offers the hope of new diagnostic, prognostic and therapeutic applications. The purpose of this review is to describe the basic principles of microarray technology and techniques and its use in pancreatic cancer research for drug discovery and development, with a focus on the relevant published data obtained from the literature. | {
"pile_set_name": "PubMed Abstracts"
} |
Considerations on the treatment of anterior interosseous nerve syndrome.
Anterior interosseous syndrome (Kiloh-Nevin syndrome) refers to that constellation of signs and symptoms referable to weakness of the pronator quadratus, the flexor pollicis longus, and the flexor digitorum profundus to the index finger.We present our series of 9 patients, affected by AIN Syndrome, and a group of 4 patients affected by pseudo-AIN neuropathies.In the literature there is considerable controversy concerning the treatment, but we agree that understanding of anatomical variants of innervation combined with a thorough physical examination can provide important clues as to where pathology resides. Proper treatment needs a precise and accurate diagnosis; in fact medical treatment which we present is effective for nerve dysfunction and may avoid surgery, but surgical exploration is mandatory when EMG is suggestive of a severe lesion, and localizes the specific site on anterior interosseous nerve entrapment. | {
"pile_set_name": "PubMed Abstracts"
} |
Emerging role of Centella asiatica in improving age-related neurological antioxidant status.
Free radicals have been hypothesized to play an important role in ageing process. There exists an imbalance between free radical production and antioxidant defense mechanism, which may lead to cell death during ageing. Our study was designed to determine whether extract of Centella asiatica, an antioxidant, when administered orally (300 mg/kg body weight/day) for 60 days would prevent age-related changes in antioxidant defense system, lipid peroxidation (LPO) and protein carbonyl (PCO) content in rat brain regions such as cortex, hypothalamus, striatum, cerebellum and hippocampus. Aged rats elicited a significant decline in the antioxidant status and increased the LPO and PCO as compared to control rats in all five regions studied. The increase in LPO and PCO contents were (64%, 34%) in cortex, (86%, 30%) in cerebellum, (51%, 47%) in striatum, (77%, 27%) in hypothalamus and (58%, 45%) in hippocampus, respectively, in aged rats as compared to young rats. Supplementation of C. asiatica was effective in reducing brain regional LPO and PCO levels and in increasing the antioxidant status. Thus, C. asiatica by acting as a potent antioxidant exerted significant neuroprotective effect and proved efficacious in protecting rat brain against age related oxidative damage. | {
"pile_set_name": "PubMed Abstracts"
} |
Beta-adrenergic receptors in human leukocyte subpopulations.
beta 2-Adrenergic receptors, which are encoded on chromosome 5q32-34, belong to the group of G-protein-linked hormone receptors with seven transmembrane domains. Upon agonist binding, adenylate cyclase is activated. Although the function of human leukocyte beta-adrenergic receptors is unknown, these cells are often used as a model system to study tissue beta-adrenergic receptors. In intact mononuclear leukocytes or membrane preparations, 1000-3000 beta 2-adrenergic receptors are found, having an antagonist affinity constant (KD) in the range of 25 pM. beta-Adrenergic receptor numbers are different in leukocyte subsets, with receptor density higher in B than in T cells. CD56+ or CD57+ natural killer cells express more receptors than CD8+ or CD4+ cells. KD is higher in CD8+ than in CD4+ cells. Acute sympathetic activation by isoproterenol infusion or short-lasting exercise leads to an increased number of mononuclear beta-adrenergic receptors with a slightly reduced proportion of those with high agonist affinity. Acute sympathetic activation by adrenaline infusion, short-term exercise, or psychological stress also causes a selective increase in circulating CD56+ or CD57+ lymphocytes which are rich in beta-adrenergic receptors. The results of several studies suggest that adrenaline-induced changes in beta-adrenergic receptors and the redistribution of leukocyte subsets may be linked. beta-Adrenergic receptors may mediate immuno-modulatory effects by causing selective cell mobilization. | {
"pile_set_name": "PubMed Abstracts"
} |
Lysozyme fibrillation: deep UV Raman spectroscopic characterization of protein structural transformation.
Deep ultraviolet resonance Raman spectroscopy was demonstrated to be a powerful tool for structural characterization of protein at all stages of fibril formation. The evolution of the protein secondary structure as well as the local environment of phenylalanine, a natural deep ultraviolet Raman marker, was documented for the fibrillation of lysozyme. Concentration-independent irreversible helix melting was quantitatively characterized as the first step of the fibrillation. The native lysozyme composed initially of 32% helix transforms monoexponentially to an unfolded intermediate with 6% helix with a characteristic time of 29 h. The local environment of phenylalanine residues changes concomitantly with the secondary structure transformation. The phenylalanine residues in lysozyme fibrils are accessible to solvent in contrast to those in the native protein. | {
"pile_set_name": "PubMed Abstracts"
} |
Assessment of mindfulness by self-report.
Assessment of mindfulness is essential to understanding its relationships with psychological functioning. Currently, mindfulness is most often assessed with self-report questionnaires. Although additional work is required, mindfulness questionnaires have reasonable psychometric properties and are making important contributions to the understanding of mindfulness and its effects on health and wellbeing. For example, measurement of mindfulness as a multidimensional construct shows that present-moment awareness can be unhelpful unless accompanied by a nonjudgmental, nonreactive stance; moreover, nonjudgment and nonreactivity may be only weakly related to present-moment awareness in people with no meditation experience. Differences between psychological and Buddhist conceptions of mindfulness, though often a source of criticism of mindfulness questionnaires, are argued here to be inevitable and not necessarily problematic. | {
"pile_set_name": "PubMed Abstracts"
} |
Community health centers: a promising venue for supplemental nutrition assistance program education in the Central Valley.
Health care providers could help achieve the necessary shift to healthful eating and active living; however, lack of coverage or reimbursement, lack of time, and limited information about appropriate interventions are some of the documented barriers. This report highlights the potential for Supplemental Nutrition Assistance Program Education (SNAP-Ed) implementation in the relatively nontraditional setting of Federally Qualified Health Centers based on the experience of the Central Valley Health Network's Nutrition Education Demonstration Project. The report provides a brief overview of the primary prevention role(s) suggested for health care providers, relevant SNAP-Ed policies, how SNAP-Ed has been implemented in Federally Qualified Health Center settings, and recommendations for similar efforts. | {
"pile_set_name": "PubMed Abstracts"
} |
Analytical performance of the serum free light chain assay.
The Freelite system for nephelometric or turbidimetric measurement of serum free light chains (FLCs) has been available since 2001. It has been valuable for the management of patients with oligosecretory myeloma, light chain myeloma and AL amyloidosis. However, there are several limitations of the method. The goal of this study was to evaluate the analytical performance of the FLC assay. Titrated controls and clinical serum specimens were used to determine precision and post-dilution recovery. As reported elsewhere, we found that the assay had several limitations, including poor post-dilution linearity and overestimation by nephelometry. These data demonstrate that the results of the FLC assay must be interpreted jointly by the clinician and the biologist, taking into account the individual patient's clinical and biological characteristics. | {
"pile_set_name": "PubMed Abstracts"
} |
Alterations of metallothionein isomers in Hg(0)-exposed rat brain.
Previously we found that exposure to mercury vapor effectively induced brain metallothionein (MT) in rats. Here, using FPLC-gel chromatography, we examined time-dependent alterations in the MT isomers, MT-I/II and MT-III, following 3 weeks of exposure. Rats were exposed to mercury vapor at 8.3 mg/m3 for 15 h in total over 5 consecutive days. Total MT levels in rat cerebrum and cerebellum increased by 65% and 155%, respectively, 24 h after the final exposure. The increased levels in both tissues remained unchanged for at least 2 weeks after termination of exposure. Interestingly, most MT in control rat cerebrum and cerebellum was accounted for by MT-III, with MT-I/II being less than 10%. Through mercury vapor exposure, MT-I/II was quickly induced to a significant extent in both tissues, reaching a level comparable to that of MT-III. The induction rate of MT-I/II in the cerebellum was somewhat higher than in the cerebrum. Chromatograms showed that the MT-I/II thus induced began to decline at an early stage in both tissues. In the cerebrum, the amount of MT-I/II on day 22 was about 30% of the maximum level on day 1. On the other hand, the induction of MT-III was not that dramatic, but it did become evident, at least in the latter stage, when MT-I/II had begun to decrease. Thus, though the induction rate of MT-III was not as high as MT-I/II, it was sustained throughout the experimental period. | {
"pile_set_name": "PubMed Abstracts"
} |
Efficient Filtration of Effluent Organic Matter by Polycation-Clay Composite Sorbents: Effect of Polycation Configuration on Pharmaceutical Removal.
Hybrid polycation-clay composites, based on methylated poly vinylpyridinium, were optimized as sorbents for secondary effluent organic matter (EfOM) including emerging micropollutants. Composite structure was tuned by solution ionic strength and characterized by zeta potential, FTIR, X-ray diffraction, and thermal gravimetric analyses. An increase in ionic strength induced a transition from a train to a loops and tails configuration, accompanied by greater polycation adsorption. Composite charge reversal (zeta potential -18 to 45 mV) increased the adsorption of EfOM and humic acid (HA), moderately and sharply, respectively, suggesting electrostatic and also nonspecific interactions with EfOM. Filtration of EfOM by columns of positively charged composites was superior to that of granular activated carbon (GAC). The overall removal of EfOM was most efficient by the composite with a train configuration. Whereas a composite with a loops and tails configuration was beneficial for the removal of the anionic micropollutants diclofenac, gemfibrozil and ibuprofen from EfOM. These new findings suggest that the loops and tails may offer unique binding sites for small micropollutants which are overseen by the bulk EfOM. Furthermore, they may explain our previous observations that in the presence of dissolved organic matter, micropollutant filtration by GAC columns was reduced, while their filtration by composite columns remained high. | {
"pile_set_name": "PubMed Abstracts"
} |
[Urine specific gravity during general anesthesia].
The urine volume and urine specific gravity per unit time during a short operation under volume loading were examined to explore the possibility of urine specific gravity as an indirect index of fluid therapy. After introducing anesthesia, under volume loading with acetic acid Ringer solution 10 ml x kg(-1) x hr(-1), urine volume and specific gravity per unit time at 30 min intervals from urination to 90 min later and the correlation between the urine volume and urine specific gravity were examined. A chronological increase of urine specific gravity was also confirmed. Therefore, the values of urine specific gravity of each 30 minute intervals were compared using Student's t-test. The urine volume and urine specific gravity per unit time showed no significant negative correlation until after 90 min. Increasing urine specific gravity even under volume loading became significant 90 min after urination. There is a possibility of employing urine specific gravity as an indirect index of fluid therapy. | {
"pile_set_name": "PubMed Abstracts"
} |
Evolutionary balancing of fitness-limiting factors.
Debates concerning the roles of different factors that may limit an organism's reproductive success pervade evolutionary ecology. We suggest that a broad class of limiting-factors problems involving essential resources or essential components of reproductive effort can be analyzed with an evolutionary application of Liebig's law of the minimum. We explore life-history evolution using the metaphor of an organism that must harvest two essential resources (resources 1 and 2) from a stochastically varying environment. Our models make three predictions. First, organisms should overinvest, relative to the deterministic case, in harvesting the resource whose per-offspring harvest cost is smaller. Second, at the optimum, organisms balance multiple fitness-limiting factors rather than being consistently limited by one factor. Third, the optimal investment in harvesting a resource is directly linked to the probability that the organism's fitness will be limited by that resource. Under temporal variation, the optimal proportional investment in harvesting resource 1 is equal to the probability that resource 1 will limit fitness. Our results help to explain why the responses of populations to environmental perturbations are hard to predict: as an organism transitions between different limiting factors, its responses to perturbations of those factors will likewise change. | {
"pile_set_name": "PubMed Abstracts"
} |
Coarse brown rice increases fecal and large bowel short-chain fatty acids and starch but lowers calcium in the large bowel of pigs.
Young male pigs were fed a diet formulated from human foods including either boiled white rice plus rice bran or heat-stabilized brown rice at equivalent levels of fiber for 3 wk. Stool and starch excretion were low in pigs fed white rice during the first 2 wk of the experiment. In pigs fed brown rice, their excretion was high during wk 1 but declined in wk 2 while short-chain fatty acid (SCFA) excretion was higher at both times. Large bowel digesta mass, measured during wk 3, was higher in pigs fed brown rice but only in the proximal colon. Large bowel and fecal starch concentrations were higher in pigs fed brown rice but the difference was insufficient to explain the increase in large bowel digesta mass. In pigs with a cecal cannula, digesta starch concentrations were equally higher when white or brown rice was fed compared with the corresponding rice which had been finely milled, indicating that particle size was a determinant of ileal digestibility. Concentrations and pools of total and individual SCFA were higher in all regions of the colon but not the cecum of pigs fed brown rice. Large bowel Ca(2+) concentrations were lower in pigs fed brown rice, suggesting greater absorption. The data confirm earlier findings that brown rice raises large bowel digesta mass and SCFA through greater fermentation of starch but show that starch itself makes a relatively small contribution to digesta and stool mass. Apparently, the rate of passage of digesta is a determinant of the concentrations and pools of SCFA in the distal colon and in feces. | {
"pile_set_name": "PubMed Abstracts"
} |
Comparing infant and juvenile behavior in bonobos (Pan paniscus) and chimpanzees (Pan troglodytes): a preliminary study.
The dichotomy between the two Pan species, the bonobo (Pan paniscus) and chimpanzee (Pan troglodytes) has been strongly emphasized until very recently. Given that most studies were primarily based on adult individuals, we shifted the "continuity versus discontinuity" discussion to the infant and juvenile stage. Our aim was to test quantitatively, some conflicting statements made in literature considering species differences between immature bonobos and chimpanzees. On one hand it is suggested that infant bonobos show retardation in motor and social development when compared with chimpanzees. Additionally it is expected that the weaning process is more traumatic to chimpanzee than bonobo infants. But on the other hand the development of behaviors is expected to be very similar in both species. We observed eight mother-infant pairs of each species in several European zoos. Our preliminary research partially confirms that immature chimpanzees seem spatially more independent, spending more time at a larger distance from their mother than immature bonobos. However, the other data do not seem to support the hypothesis that bonobo infants show retardation of motor or social development. The development of solitary play, environmental exploration, social play, non-copulatory mounts and aggressive interactions do not differ between the species. Bonobo infants in general even groom other group members more than chimpanzee infants. We also found that older bonobo infants have more nipple contact than same aged chimpanzees and that the weaning process seems to end later for bonobos than for immature chimpanzee. Additionally, although immature bonobos show in general more signs of distress, our data suggest that the weaning period itself is more traumatic for chimpanzees. | {
"pile_set_name": "PubMed Abstracts"
} |
Early initiation of immunosuppressive treatment in membranous nephropathy patients.
Suggestion for the management of idiopathic membranous nephropathy (IMN) includes 6 months of observation, followed with steroid plus alkylating agent. However, delayed immunosuppression exposes the kidneys to persistent damage. This study aimed to examine the benefit of early immunosuppression in IMN patients. A retrospective study was performed. From 1993 to 2013, 161 IMN patients were enrolled. Patients receiving immunosuppression within 6 months after diagnosis were classified as initial-treatment group, whereas other patients as initial-no-treatment group. The clinical outcomes and complication were examined. Patients in the initial-treatment group had lower serum albumin concentration, less diabetes, and were younger. Steroid monotherapy is the main immunosuppression (64.5%) in this group. The initial-treatment group had a higher complete and partial remission rate than the initial-no-treatment group 6 months (52.9% vs. 35.0%, p=0.05) and 12 months (71.1% vs. 45.0%, p=0.003) after diagnosis. A similar result was seen between initial-steroid monotherapy and initial-no-treatment patients. Early immunosuppression is an independent predictor of remission within 1 year [hazard ratio (HR)=2.09; 95% confidence interval (CI)=1.25-3.49; p=0.005] and estimated glomerular filtration rate (eGFR) decline over 50% during the follow-up. (HR=0.33; 95% CI=0.13-0.86; p=0.02). The initial-treatment group also had a low frequency of eGFR decline over 50% (p=0.001) and low combined end-stage renal disease/mortality (p=0.001) compared with the initial-no-treatment group, but without more immunosuppression-related complication. In contrast to Western countries, early immunosuppression (even steroid monotherapy) in our patients is associated with better remission in the 1st year and renal preserve. Further randomized controlled trials are needed to clarify the benefit of early immunosuppression in IMN patients, especially with oriental ethnic background. | {
"pile_set_name": "PubMed Abstracts"
} |
Spectrofluorimetric analysis of cefoxitin in pharmaceutical dosage.
A fluorescence method involving sample pre-treatment is investigated concerning the determination of cefoxitin. A fluorescent product is formed when samples containing cefoxitin are subjected to alkaline hydrolysis with 1.0M sodium hydroxide and heated for 60 min at 90 degrees . The fluorescence is measured in ethanol/water medium (50% v/v) at approximately pH 2.0 provided by adding of 0.1M hydrochloric acid. The fluorescence excitation and emission maxima were 317 and 400 nm, respectively. The quantitative range is between 0.020 and 1.40 mug/ml. A detection limit of 2 x 10(-3) mug/ml was found. The proposed method has been applied to the determination of cefoxitin in commercial injections, saline and glucosed physiological serum. | {
"pile_set_name": "PubMed Abstracts"
} |
[Gastric and alveolar ruptures in severe ketoacidosis (author's transl)].
Severe ketoacidosis can be complicated by alveolar and gastric ruptures. A knowledge of the pathophysiological mechanisms which may lead to these threatening clinical pictures enables the radiologist to give useful suggestions in the emergency diagnosis, as shown in the examples of two patients here. | {
"pile_set_name": "PubMed Abstracts"
} |
Molecular diagnosis of cystic fibrosis: comparison of four analytical procedures.
We compared the analytical accuracy, times, costs and the detection rate of four procedures for the molecular analysis of cystic fibrosis (CF). DNA from 127 subjects bearing different genotypes was tested by denaturating gradient gel electrophoresis followed by sequencing (reference procedure) and, for comparison, by ASO dot-blot (in-house), reverse dot-blot (Innogenetics), ARMS (Zeneca Diagnostics) and OLA-PCR (PE Applied Biosystem). To assess inter-observer variability, all samples were tested twice. To evaluate intra- and between-series variability, two samples were tested twice in each series. All the procedures yielded reproducible results and assigned the correct genotype to each sample. ASO dot-blot is the cheapest procedure but has the longest analytical time (> 24 h) and uses radioactive labeling. It can be adapted to analyze peculiar mutations in specific ethnic groups. ARMS from Zeneca Diagnostics is rapid (4 h), easy to perform, but, except for the AF508 mutation, does not distinguish the homozygote from the heterozygote genotype. It could be used for carrier analysis in families with known mutations. OLA-PCR has the highest detection rate in most ethnic groups, is automated for capillary electrophoresis but requires a high level of operator expertise: it is suitable when collected series of samples are analyzed from large patient cohorts. Reverse dot-blot is easy to perform and can be semiautomated: it can be used as first-line screening test. Given the heterogeneity of CF mutations, commercial kits should be developed to analyze mutations peculiar to specific ethnic groups. | {
"pile_set_name": "PubMed Abstracts"
} |
Transcutaneous oxygen tension in the dysvascular foot with infection.
Eight adult insulin-requiring diabetics with peripheral vascular disease were admitted with foot infection and signs of systemic sepsis. Transcutaneous oxygen tension was measured at the foot and ankle prior to surgery. None of the values were sufficient to support wound healing. Four of the patients underwent open ray resection and four open midfoot amputation. After resolution of the local infections, transcutaneous oxygen tensions were repeated. Seven of the eight patients exhibited an appreciable increase in the value following decompression of the foot infection, sufficient to support wound healing. | {
"pile_set_name": "PubMed Abstracts"
} |
Life history evolution: what does a menopausal killer whale do?
Menopause evolved in humans and whales, presumably because older females can help their kin. But how do they help? New research shows that post-menopausal female killer whales lead foraging groups. This leadership is most significant when food is scarce. | {
"pile_set_name": "PubMed Abstracts"
} |
Time-series analysis of satellite-derived fine particulate matter pollution and asthma morbidity in Jackson, MS.
In order to examine associations between asthma morbidity and local ambient air pollution in an area with relatively low levels of pollution, we conducted a time-series analysis of asthma hospital admissions and fine particulate matter pollution (PM2.5) in and around Jackson, MS, for the period 2003 to 2011. Daily patient-level records were obtained from the Mississippi State Department of Health (MSDH) Asthma Surveillance System. Patient geolocations were aggregated into a grid with 0.1° × 0.1° resolution within the Jackson Metropolitan Statistical Area. Daily PM2.5 concentrations were estimated via machine-learning algorithms with remotely sensed aerosol optical depth and other associated parameters as inputs. Controlling for long-term temporal trends and meteorology, we estimated a 7.2% (95% confidence interval 1.7-13.1%) increase in daily all-age asthma emergency room admissions per 10 μg/m3 increase in the 3-day average of PM2.5 levels (current day and two prior days). Stratified analyses reveal significant associations between asthma and 3-day average PM2.5 for males and blacks. Our results contribute to the current epidemiologic evidence on the association between acute ambient air pollution exposure and asthma morbidity, even in an area characterized by relatively good air quality. | {
"pile_set_name": "PubMed Abstracts"
} |
Arthroscopy in acute septic knees. Management in pediatric patients.
Arthroscopic evacuation, debridement, and irrigation of acute septic knees in children were effective adjuncts in the treatment of this joint infection. Sixteen knees in 16 pediatric patients were treated with arthroscopic management. Ninety-four percent of the knees had sepsis secondary to Staphylococcus aureus. Two immune-suppressed patients (postrenal transplants) had combined S. aureus and Pseudomonas aeruginosa infections. Twenty-five percent of the patients had a foreign body in the joint that was removed arthroscopically. No postoperative irrigation or drainage systems were used. At an average follow-up period of three years, no evidence of persistent or recurrent infection was noted. Roentgenograms showed no evidence of joint or epiphyseal destruction. Because of the low morbidity associated with the procedure, rapid restoration of joint motion and patient mobility was seen in all patients. Arthroscopic debridement of acute septic knees may be carried out in pediatric patients, given appropriate equipment and arthroscopic skills. | {
"pile_set_name": "PubMed Abstracts"
} |
[Transsacral resection for presacral tumors].
To explore the operation indication and safety of presacral tumor. Clinical data of 36 patients with presacral tumor from November 1990 to May 2006 treated in our hospital, in whom 23 patients underwent trans-sacral operation, were analyzed retrospectively. The operation time was from 43 to 210 min (average 94 min). The volume of blood loss was from 30 to 2000 ml (average 350 ml). Hospital stay was from 8 to 16 days (average 10.7 days). There were 13 different pathology types of tumors in the 36 patients including 26.4% of malignancy. Complications of trans-sacral operation included 1 case of ureteral damage, 1 case of sacral wound hernia, 1 case of presacral abscess who was healed by sigmoid stoma and wound drainage. Trans-sacral resection of low presacral tumor is safe and effective with less trauma, less bleeding and quick recovery. | {
"pile_set_name": "PubMed Abstracts"
} |
Identification of seven polyamine oxidase genes in tomato (Solanum lycopersicum L.) and their expression profiles under physiological and various stress conditions.
Polyamines (PAs) are implicated in developmental processes and stress responses of plants. Polyamine oxidases (PAOs), flavin adenine dinucleotide-dependent enzymes that function in PA catabolism, play a critical role. Even though PAO gene families of Arabidopsis and rice have been intensely characterized and their expression in response to developmental and environmental changes has been investigated, little is known about PAOs in tomato (Solanum lycopersicum). We found seven PAO genes in S. lycopersicum and named them SlPAO1∼7. Plant PAOs form four clades in phylogenetic analysis, of which SlPAO1 belongs to clade-I, SlPAO6 and SlPAO7 to clade-III, and the residual four (SlPAO2∼5) to clade-IV, while none belongs to clade-II. All the clade-IV members in tomato also retain the putative peroxisomal-targeting signals in their carboxy termini, suggesting their peroxisome localization. SlPAO1 to SlPAO5 genes consist of 10 exons and 9 introns, while SlPAO6 and SlPAO7 are intronless genes. To address the individual roles of SlPAOs, we analyzed their expression in various tissues and during flowering and fruit development. The expression of SlPAO2∼4 was constitutively high, while that of the other SlPAO members was relatively lower. We further analyzed the expressional changes of SlPAOs upon abiotic stresses, oxidative stresses, phytohormone application, and PA application. Based on the data obtained, we discuss the distinctive roles of SlPAOs. | {
"pile_set_name": "PubMed Abstracts"
} |
Effect of tamoxifen on mammographic density.
There are strong data showing that increased breast cancer risk is associated with increased mammographic density. Tamoxifen has been shown to decrease the risk of invasive breast cancer and decrease breast density. We sought to demonstrate and calculate the extent of change in mammographic density in women who have taken tamoxifen for up to 2 years. We evaluated mammograms from 28 high-risk women who were taking tamoxifen. Four different methods of evaluation were used: (a) two qualitative methods (Wolfe criteria and the American College of Radiology Breast Imaging and Reporting Data System criteria); (b) one semiquantitative method (mammograms were assigned one of five semiquantitative scores by visual inspection); and (c) one quantitative method (computer-aided calculation of fibroglandular area from digitized mammograms). The Wolfe criteria showed a 0.03 category decrease per year (P = 0.50). The American College of Radiology Breast Imaging and Reporting Data System criteria showed a 0.1 category decrease per year (P = 0.12). Semiquantitative criteria showed a 0.2 category decrease per year (P = 0.039). Digitized scores showed a 4.3% decrease per year (P = 0.0007). In conclusion, tamoxifen causes a decrease in mammographic density with use, an effect that is better quantitated with semiquantitative criteria or digitized images. Density change might become useful as a surrogate end point for the effect of tamoxifen and other chemopreventive measures, although our data do not predict an individual's degree of risk reduction. | {
"pile_set_name": "PubMed Abstracts"
} |
Assessment of speech perception and communication skills in adolescents with cochlear implants for pre- and peri-lingual deafness.
This study aimed to assess speech perception and communication skills in adolescents between ages 8 and 18 that received cochlear implants for pre- and peri-lingual deafness. We studied 15 adolescents, aged 12 to 23 years, with late cochlear implantation. Speech perception was assessed with the Bishop sentences test and a memory number sequence test at 3-9 years after cochlear implantation. A questionnaire completed retrospectively was used to investigate communication skills pre- and post implantation. Six individuals achieved grammar comprehension scores comparable to children 8-10 years old with normal hearing; only 3 individuals achieved a percentile rank higher than 50% in the memory number sequence test. The self-reported communication skills improved after cochlear implantation in all adolescents. Speech perception skills of adolescents with late implantation for pre- and peri-lingual deafness are typically inferior to those of children with normal hearing at the age of 10. However, when the evaluation of the cochlear implant outcome was broadened with the use of a questionnaire, many individuals reported that they participated more actively in conversations, spoke more actively to unknown individuals, and were more easily understood by others. | {
"pile_set_name": "PubMed Abstracts"
} |
Direct carbamoylation of alkenyl halides.
Alkenyl chlorides and bromides are converted into tertiary enamides by treatment with a carbamoylsilane in toluene at 110 degrees C in the presence of phosphine-palladium(0) catalysts. [reaction: see text] | {
"pile_set_name": "PubMed Abstracts"
} |
Dissociation of the peptide/MHC class I complex: pH dependence and effect of endogenous peptides on the activation energy.
Dansylated peptides were used to characterize the dissociation of peptides from a recombinant class I major histocompatibility complex protein. Dissociation of endogenous, low-affinity peptides from the class I molecule Kd had an activation energy of 6.78 +/- 0.64 kcal/mol in the 14 to 26 degrees C temperature range, but there was a break in the Arrhenius plot between 12 and 14 degrees C. Dissociation of a dansylated, high-affinity peptide had an activation energy of 20.24 +/- 1.69 kcal/mol, and there was similarly a break in the plot. Both direct interactions between peptide and the class I heavy chain and indirect effects of the peptide affinity on the extent of light chain association with heavy chain may contribute to the difference in activation energies, while the break in the Arrhenius plots implies a temperature-dependent conformational change. Dissociation was also slowest at neutral pH, but the peptide/class I complex dissociated rapidly at pH greater than 9 and less than 5, suggesting that endocytosed class I proteins would most likely lose their bound peptides at the acidic pH of endosomes. | {
"pile_set_name": "PubMed Abstracts"
} |
Association of adriamycin-induced resistance to NK-mediated lysis with sialic acid level and immunological reactivity of transferrin receptors and glycophorin A.
Adriamycin (ADM) can increase sialic acid content in K 562 cells and reduce their susceptibility to NK-mediated lysis. In this report, hypothetical relationship between this resistance and augmentation in sialylation has been investigated. Variations in the time of exposure to ADM showed that 12 hours were sufficient to cause maximal recruitment of benzidine-positive cells, growth inhibition and resistance to NK-mediated lysis. On the contrary, the membrane sialic acid density seemed stable and 24 hours of drug exposure were necessary to observe a clear rise in sialic acid. Neuraminidase treatment of control and ADM-treated K 562 cells was associated with an obvious enhancement in their susceptibility to NK-mediated lysis which can be explained by an increase in the target-effector binding ability as assessed by a direct conjugate-forming cell assay. However, the neuraminidase treatment did not modify the sensitivity difference to lysis between untreated and ADM-treated cells. As compared to control the reactivity of ADM-treated cells was higher with an antiglycophorin A (GPA) MAb and lower with an antitransferrin receptor (TFR) MAb. Kinetic studies suggested that GPA expression is a better index of ADM-induced resistance to NK-mediated lysis than TFR expression. In addition, neuraminidase treatment showed that TFR and GPA modulations induced by ADM can be correlated with sialylation alterations. | {
"pile_set_name": "PubMed Abstracts"
} |
Absorption effects in liquid crystal waveguides.
An analytical and numerical study of the propagation of optical fields through a nematic hybrid slab is developed. We take into account explicitly the absorption of radiation by the liquid crystal by introducing a complex dielectric tensor. For a low intensity beam we first derive the eikonal equation and from it we calculate the ray trajectories in the optical limit. We show that in the presence of absorption, there are no caustics within the slab. Then we consider the WKB limit and calculate the field transverse magnetic modes, their number and their cutoff frequencies. We show that for both limits the agreement between our analytical and numerical results for the propagation constants is excellent, while there are larger differences in the analytically and numerically calculated field amplitudes. These differences show that absorption effects are important for this quantity and have their origin in the fact that the chosen parameter values in our exact numerical calculations, strictly speaking, do not lie within the limits of validity of the WKB approximation. Although a more precise comparison between these approaches requires the use of different sets of values of the relevant parameters, our analysis shows the effects and complications arising from the inclusion of absorption. Finally, we discuss the scope and limitations of our approach. | {
"pile_set_name": "PubMed Abstracts"
} |
Randomized clinical trial of Ligasure versus open haemorrhoidectomy.
Postoperative pain associated with open haemorrhoidectomy remains problematic. Haemorrhoidectomy performed using bloodless bipolar diathermy--Ligasure--may have advantages over conventional open haemorrhoidectomy in terms of operating time and postoperative pain. Thirty-four patients were randomized to undergo Ligasure (18 patients) or diathermy (16) haemorrhoidectomy. The operating time, amount of pain and postoperative analgesic requirement, postoperative complications and overall patient satisfaction were documented. The median duration of operation was shorter in the Ligasure haemorrhoidectomy group (5.1 versus 9.2 min; P < 0.001). There was no statistically significant difference in the postoperative pain score, but the median analgesic requirement was lower in the Ligasure group (850 versus 1600 mg tramadol; P = 0.013). Patient satisfaction was similar in both groups. Ligasure haemorrhoidectomy is quick and bloodless and, although as painful as diathermy haemorrhoidectomy, is associated with a reduced analgesic requirement. | {
"pile_set_name": "PubMed Abstracts"
} |
Modulation of the activity of endogenous protein kinase inhibitors in rat heart by the beta adrenergic receptor.
The rat heart contains a large amount of two protein kinase inhibitors. The type I inhibitor blocks the activity of cAMP-dependent protein kinase while the type II inhibitor blocks cGMP-dependent protein kinase, cAMP-dependent protein kinase and cyclic nucleotide-independent protein kinase. Isoproterenol produced a dose-dependent decrease of the type I inhibitor activity and an increase of cAMP content in rat heart. The decrease of the type I inhibitor activity is mediated through adrenergic beta receptor because propranolol and practolol but not phentolamine prevented the effects of isoproterenol. Moreover, prior treatment with aminophylline markedly enhanced isoproterenol-induced increase of cAMP content and decrease of the type I inhibitor activity. Isoproterenol did not change the activity of the type II inhibitor. These results are compatible with the hypothesis that the neurotransmitter generated cAMP modulates phosphorylation in the heart by changing the relationship between cAMP-dependent protein kinase and the type I inhibitor activity. | {
"pile_set_name": "PubMed Abstracts"
} |
Calculating the concentration index when income is grouped.
The problem introduced by grouping income data when measuring socioeconomic inequalities in health (and health care) has been highlighted in a recent study in this journal. We re-examine this issue and show there is a tendency to underestimate the concentration index at an increasing rate when lowering the number of income categories. This tendency arises due to a form of measurement error and we propose two correction methods. Firstly, the use of instrumental variables (IV) can reduce the error within income categories. Secondly, through a simple formula for correction that is based only on the number of groups. We find that the simple correction formula reduces the impact of grouping and always outperforms the IV approach. Use of this correction can substantially improve comparisons of the concentration index both across countries and across time. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of Zr doping on stoichiometric and reduced ceria: a first-principles study.
The Zr doping in CeO(2) may change the reduction properties and therefore the redox properties of CeO(2). Using first-principles density functional theory with the inclusion of on-site Coulomb interaction for a 96-atom supercell, these effects are studied by comparing the differences in atomic structures, electronic structures, and reduction energies of the doped CeO(2) and those of the nondoped CeO(2). It is found that (1) Zr doping of the ceria structure results in important modifications involving nonequivalent O atoms; (2) the oxygen anions (still four-coordinated) next to the doping center show considerably lower reduction energies (by 0.6 eV) and larger displacements ("higher mobilities"); (3) an O vacancy is most easily created close to the Zr centers, therefore the Zr-doping centers might serve as nucleation centers for vacancy clustering; and (4) the electrons left by the released oxygen localize on two Ce cations neighboring the vacancy, which results in the reduction of two Ce(4+) ions. | {
"pile_set_name": "PubMed Abstracts"
} |
[Application of Pirkle-type chiral column to the resolution of enantiomeric drugs: resolution and identification of alpha-naphthyl isocyanate derivatives of alprenolol and some other beta-blockers].
alpha-Naphthyl isocyanate (NI) as a derivatizing agent to separate several beta-blockers was described. This procedure is simple, rapid and gives good resolution. Experiments with NI derivatives of alprenolol showed that the isocyanate group reacted with the amino group on the analyte to form urea under mild conditions and with the hydroxyl group to form carbamate under relatively vigorous conditions. The results also indicated that the derivatized reactions is 1:1 between NT and analytes. | {
"pile_set_name": "PubMed Abstracts"
} |
High-Performance Supported Iridium Oxohydroxide Water Oxidation Electrocatalysts.
The synthesis of a highly active and yet stable electrocatalyst for the anodic oxygen evolution reaction (OER) remains a major challenge for acidic water splitting on an industrial scale. To address this challenge, we obtained an outstanding high-performance OER catalyst by loading Ir on conductive antimony-doped tin oxide (ATO)-nanoparticles by a microwave (MW)-assisted hydrothermal route. The obtained Ir phase was identified by using XRD as amorphous (XRD-amorphous), highly hydrated IrIII/IV oxohydroxide. To identify chemical and structural features responsible for the high activity and exceptional stability under acidic OER conditions with loadings as low as 20 μgIr cm-2 , we used stepwise thermal treatment to gradually alter the XRD-amorphous Ir phase by dehydroxylation and crystallization of IrO2 . This resulted in dramatic depletion of OER performance, indicating that the outstanding electrocatalytic properties of the MW-produced IrIII/IV oxohydroxide are prominently linked to the nature of the produced Ir phase. This finding is in contrast with the often reported stable but poor OER performance of crystalline IrO2 -based compounds produced through more classical calcination routes. Our investigation demonstrates the immense potential of Ir oxohydroxide-based OER electrocatalysts for stable high-current water electrolysis under acidic conditions. | {
"pile_set_name": "PubMed Abstracts"
} |
Evoked potential correlates of signal recognition between and within modalities.
Electrical responses evoked by clicks, flashes, changes in noise level, and changes in light level were recorded from the scalps of human subjects set to detect one of the stimuli. An early negative component of the evoked responses reflects selection between sensory modalities, whereas the later positive component reflects a more complex intramodal discrimination. | {
"pile_set_name": "PubMed Abstracts"
} |
Periodicity of long-term context can influence gap detection.
Temporal gap detection in bands of noise is a basic paradigm to investigate auditory temporal resolution. This study further examined the role of the long-term temporal organization for gap detection. Our experiments aimed at determining whether a gap superimposed on an amplitude-modulated noise was easier to detect when the amplitude modulation was regular--or periodic--than when it was not. Care was taken to ensure that the stimulus portion, where the gap was inserted, was exactly the same for both periodic and aperiodic conditions. Results show that gap detection in periodically modulated white noise (125 Hz fundamental frequency, or 8 ms duration from peak to peak) is easier (with a threshold 4.7 ms lower) than in an aperiodically modulated noise (modulation randomly varied from 5 to 18 ms). This effect is observed for variations of the modulation frequency from 125 to 1000 Hz. These results indicate that the regularity of long-term temporal organization of amplitude modulations in noise can improve the detection of a gap. | {
"pile_set_name": "PubMed Abstracts"
} |
[Detection rate on un-repaired cleft lip/palate patients in Gansu province in 2008].
To investigate the number and distribution of un-repaired cleft lip/palate patients in Gansu province. A census was conducted by staff members of the health system and Population and Family Planning Committee (PFPC) in Gansu province. Standardized - Questionnaires were administered to collect demographic, domestic and diagnosis-related information. In 2008, the total number of un-repaired cleft lip/palate patients was 4675, with a detection rate of 1.84/10 000 in Gansu province. Rates of detection were higher in males (2.11/10 000), young age group (4.86/10 000), rural areas (2.23/10 000), poor counties (2.19/10 000) than in females (1.43/10 000), medium (0.97/10 000)/old age group (0.68/10 000), township areas (0.62/10 000), or richer counties (1.35/10 000). Among all the cities and prefectures of Gansu, Baiyin city (2.7/10 000) had the highest while Jinchang city (0.7/10 000) had the lowest prevalence rates. Un-repaired cleft lip/palate had been a disease burden to Gansu province, especially in the rural area and poorer counties. | {
"pile_set_name": "PubMed Abstracts"
} |
Plasma hormones in neotropical and domestic cats undergoing routine manipulations.
Many neotropical felines are threatened with extinction and information on their physiology is required to assist in conservation. Their reproduction in captivity is poor, particularly for the smaller species. Several factors may be responsible, but stress is probably the most important. We assayed cortisol, LH, FSH, prolactin, testosterone, estradiol, and progesterone in single blood samples obtained under sedation from seven neotropical species and, for comparison, in stressed and unstressed domestic cats. Cortisol was also assayed in serial blood samples obtained after ACTH administration in Leopardus tigrinus, L. wiedi and domestic cats. While, in general, the results were fairly consistent, there were some statistically significant differences between species that were large enough to be of practical importance. | {
"pile_set_name": "PubMed Abstracts"
} |
Training with mannequins improves safety, efficiency.
Mannequin training offers viable alternative to more standard training approaches. Staff has ability to respond to the same challenging scenarios over and over again. Simulation can be used for pure teaching or for competency examinations. Training also can be used to monitor compliance with patient safety accreditation standards. | {
"pile_set_name": "PubMed Abstracts"
} |
Chromosomal mosaicism throughout human preimplantation development in vitro: incidence, type, and relevance to embryo outcome.
A large percentage of in-vitro generated cleavage stage human embryos are chromosomally mosaic, consisting of both normal (diploid) and abnormal (non-diploid) cells. The present study characterized mosaicism at each stage of cleavage division and examined its effect on preimplantation development in vitro. A total of 216 normally fertilized (two-pronucleate) embryos which were not selected for transfer to the patients were analysed for chromosomal abnormalities using multi-colour fluorescence in-situ hybridization DNA probes specific for three to five of nine different chromosomes (X, Y, 2, 7, 13, 16, 18, 21, 22). Overall, 48.1% of embryos were mosaic. The frequency of mosaic embryos increased from 15.2 to 49.4 to 58.1%, from the 2-4-cell to 5-8-cell to morula stages respectively, and the types of non-diploid cells detected were mostly aneuploid or chaotic. The incidence of mosaicism at the blastocyst stage was 90.9%; however, most of the mosaicism comprised diploid and polyploid cells. Arrested mosaic embryos had a higher incidence of chaotic abnormalities, and higher proportions of abnormal cells compared with the non-arrested group. Post-zygotic errors leading to mosaicism may occur, and persist throughout preimplantation development in vitro. Our results suggest that mosaicism involving multiple chromosomal imbalances and/or imbalances affecting a high proportion of cells in an embryo appear to impair development to the blastocyst stage. | {
"pile_set_name": "PubMed Abstracts"
} |
The association between dietary patterns and type 2 diabetes: a systematic review and meta-analysis of cohort studies.
Observational studies suggest that dietary pattern intake plays an important role in the development of type 2 diabetes. A meta-analysis was performed of existing cohort studies on the association between dietary patterns and the risk of type 2 diabetes. Pertinent studies were identified by searching MEDLINE, EMBASE, Cumulative Index to Nursing and Allied Health Literature, PROQUEST, MEDNAR and the Joanna Briggs Institute Library of Systematic Reviews, as well as the reference lists of all identified studies up to September 2012. A random-effects meta-analysis was performed to pool relative risk (RR) estimates from individual studies to assess the relationship between dietary pattern intake and the risk of type 2 diabetes. Statistical heterogeneity was assessed using I(2) and sensitivity analyses were conducted. Publication bias was evaluated by visual inspection of funnel plots and was formally assessed using Egger's test. There were fifteen cohort studies that met the inclusion criteria. There was evidence of a reduction in the risk of type 2 diabetes in the highest adherence compared to the lowest adherence to healthy dietary patterns [RR = 0.79, 95% confidence interval (CI) = 0.74-0.86, P < 0.005]. An increase in the risk of type 2 diabetes was evident for the highest adherence compared to the lowest adherence to unhealthy dietary patterns (RR = 1.44, 95% CI = 1.33-1.57, P < 0.005). The results of this systematic review and meta-analysis indicate that dietary patterns may be associated with the risk of type 2 diabetes. | {
"pile_set_name": "PubMed Abstracts"
} |
Serum 25-hydroxyvitamin D levels in subjects with reduced glucose tolerance and type 2 diabetes - the Tromsø OGTT-study.
The relationships between vitamin D concentrations, hyperglycemia, and insulin resistance remain uncertain. During 2008 - 2010, an oral glucose tolerance test was performed in 3520 subjects from Tromsø, Norway. Serum 25-hydroxyvitamin D [25(OH)D] was measured in 1193 subjects with normal glucose tolerance, in 304 with isolated impaired fasting glucose, in 254 with isolated impaired glucose tolerance, in 139 with a combination of the two, and in 194 subjects with type 2 diabetes. Serum 25(OH)D did not differ between subjects with isolated impaired fasting glucose or impaired glucose tolerance, but was lower in all groups of deranged glucose metabolism as compared with normal subjects. These differences could not be explained by differences in intakes of vitamin D from cod liver oil or other supplements and remained statistically significant after adjustment for gender, age, body mass index, physical activity score, and month of examination. When the cohort was divided according to serum 25(OH)D quartiles, there was an improvement in all measures of glucose metabolism (fasting and 2-hour plasma glucose, serum insulin, HbA(1c)) and estimates of insulin resistance (QUICKI , HOMA-IR, ISI(0.120)) with increasing serum 25(OH)D quartile. However, interventional studies are needed to prove a causal relationship between vitamin D and glucose metabolism. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of recombinant interferon-tau on ovine lentivirus replication.
As a pregnancy recognition signal, sheep trophoblast cells secrete a type I interferon, ovine interferon-tau (OvIFN-tau), which has potent antiviral activity. We studied the effects of a recombinant protein (rOv-IFN-tau) on the replication of ovine lentivirus (OvLV) in goat synovial membrane cells. The amount of provirus DNA, as measured by polymerase chain reaction (PCR), the virus titers, and the number of OvLV-induced syncytia were 76.5%, 82%, and 95%, respectively, lower in cultures treated with rOv-IFN-tau than in placebo-treated controls (p < 0.01). rOv-IFN-tau also reduced OvLV reverse transcriptase activity and protected cells from OvLV-induced cell lysis, but the effect was less dramatic. The antiviral activity increased with the concentration up to a maximum with 256 antiviral units of rOv-IFN-tau per ml. | {
"pile_set_name": "PubMed Abstracts"
} |
Continuous positive airways pressure for obstructive sleep apnoea.
Obstructive sleep apnoea is the periodic reduction (hypopnoea) or cessation (apnoea) of breathing due to narrowing or occlusion of the upper airway during sleep. The main symptom is daytime sleepiness although there it has been linked to premature death, hypertension, ischaemic heart disease, stroke and road traffic accidents. The main treatment for sleep apnoea is with continuous positive airways pressure (CPAP) treatment, which consists of a flow generator and mask. These are used at night to prevent apnoea, hypoxia and sleep disturbance. The objective was to assess the effects of CPAP in the treatment of obstructive sleep apnoea in adults. We searched the Cochrane Airways Group RCT register (MEDLINE 1966 to 2000, Embase 1974 to 2000, Cinahl 1982 to 2000) and the reference lists of articles. We consulted experts in the field. Randomised trials comparing nocturnal CPAP with placebo or other treatments in adults with obstructive sleep apnoea and an apnoea/hypopnoea index greater than five per hour. Trial quality was assessed and two reviewers extracted data independently. Study authors were contacted for missing information. Twelve trials involving 475 people were included. Most studies had methodological shortcomings. Most trials were of crossover design. Compared with placebo, CPAP showed significant improvements in objective and subjective sleepiness and several quality of life and depression measures. Patients preferred CPAP to placebo (odds ratio 0.4, 95% confidence interval 0.2 to 0.8). There was no significant effect on daytime blood pressure. Compared with oral appliances, CPAP significantly improved the apnoea/hypopnoea index (weighted mean difference -7.3, 95% confidence interval -10.0 to -4.7) and minimum oxygen saturation during sleep. Patients strongly preferred the oral appliance to CPAP (odds ratio 9.5, 95% confidence interval 4.3 to 21.1). CPAP is more effective than placebo in improving sleepiness and quality of life measures for people with obstructive sleep apnoea. It is more effective than oral appliances in improving respiratory disturbances. Although patients prefer CPAP to placebo, they preference oral appliances to CPAP. | {
"pile_set_name": "PubMed Abstracts"
} |
Experimental parathyroid transplantation: human parathyroid grafts survived and functioned in mice treated with anti-CD4 monoclonal antibody.
Permanent hypoparathyroidism is one of the most difficult of all endocrine disorders to treat medically. To examine the possibility that xenotransplantation can be used to treat hypoparathyroidism, human parathyroid tissues were transplanted into mice. Human parathyroid tissue was taken from specimens excised from patients with hyperparathyroidism. Fresh human parathyroid tissue was implanted under kidney capsule of CBA (H2k) mice. Some mice were treated intraperitoneally with depleting anti-CD4 monoclonal antibody (mAb, YTA 3.1, 100 microg/dose, days -1. 0. 1, 2, 3, and 5). Mice were killed 30 days after transplantation. Survival of parathyroid grafts was examined microscopically and human parathyroid hormone in serum was measured by ELISA. All parathyroid grafts survived under kidney capsule and human parathyroid hormone was strongly detected in serum (621 +/- 576 pg/mL) when recipients were treated with short-course treatment of anti-CD4 mAb. Conversely, no parathyroid tissue was seen microscopically in any recipient mice without anti-CD4 mAb treatment. Human parathyroid hormone was undetectable by ELISA in naive mice and mice transplanted with human parathyroid tissue without short-course treatment of anti-CD4 mAb. Xenogeneic human parathyroid tissue survived and functioned in mice treated with short-course treatment of anti-CD4 mAb. | {
"pile_set_name": "PubMed Abstracts"
} |
Students as teachers in an anatomy dissection course.
One way to improve students' learning outcomes and well-being is to change teaching practices to allow students to become more active participants. We used an anatomy dissection course to test a cooperative group work method in which first-year veterinary students took turns leading their peer group and were each responsible for teaching the anatomy of a particular topographic region. The important blood vessels, lymphatic system, and nerves of each region of the body were covered. Students felt that exploration of the entire topographic region helped them to acquire a comprehensive understanding of the respiratory apparatus and the cardiovascular and nervous systems. Assigning individual tasks to each group member resulted in sharing the workload equally. Open-ended comments revealed that support from other group members was important for the students' learning experience, but the results also offered insight into a lack of constructive criticism. While teaching was considered challenging, and even a stress factor that hindered learning for some students, group work was generally held to be supportive of learning. The results suggest that more thorough instruction of students in their group work and in their individual tasks is required. Some students experienced difficulty in expressing their concerns openly and in seeking guidance from teachers, demonstrating the need for further investigation regarding students' self-regulation skills. Comments from the open-ended responses suggest that use of a cooperative learning method in anatomy dissection courses not only deepens student understanding of a subject but also offers first-year students an opportunity to practice the generic skills that will be needed in their future profession. | {
"pile_set_name": "PubMed Abstracts"
} |
What the MAYSI-2 Can Tell Us About Anger-Irritability and Trauma.
The rate of experiencing trauma can be quite high for juvenile offenders and those experiences can lead to feelings of anger and irritability. This study uses Massachusetts Youth Screening Instrument-Version 2 (MAYSI-2) data to examine the relationship between the Traumatic Experiences (TE) and Angry-Irritable (AI) scales for juvenile offenders (N = 1,348) from a Midwest detention center. Chi-square analyses revealed statistically significant relationships between the AI scale and gender, race, and the TE scale. In addition, ordinal logistic regression results showed that as the number of traumatic experiences increased, so did the odds of scoring Caution or Warning on the AI scale. Recommendations are that juvenile justice systems utilize a trauma-informed process throughout the adjudicatory process and there be improved efforts to coordinate services across multiple systems, such as child welfare and special education, where juvenile offenders are often engaged. | {
"pile_set_name": "PubMed Abstracts"
} |
Serum prolactin response to thyrotropin-releasing hormone during status epilepticus.
Determination of serum prolactin can help distinguish between epileptic and pseudo-epileptic attacks since generalized tonic-clonic and complex partial seizures frequently are accompanied by a transient rise in prolactin. In status epilepticus, however, serum prolactin levels are well within the normal range: cellular depletion due to the prolonged seizure activity has been suggested as a mechanism for this finding. The control of prolactin secretion is complex. Among several possible regulators, inhibitory dopamine and stimulatory thyrotropin-releasing hormone (TRH) may take part in the regulation of prolactin levels in connection with epileptic activity. There may be subpopulations of prolactin-producing cells that react differently in response to various regulators. A dopamine receptor blocker given during status epilepticus brings forth a distinct increase in prolactin levels. In order to add to the understanding of prolactin changes in connection with status epilepticus, we injected TRH i.v. during status epilepticus in seven consecutive patients. All patients had prolactin levels within the normal range (< 25 micrograms/l) before injection of TRH which resulted in at least a two-fold increase in prolactin levels. Our results contradict the hypothesis of cellular depletion of prolactin in connection with status epilepticus. The mechanism behind prolactin values within the normal range after prolonged seizure activity remains unknown. | {
"pile_set_name": "PubMed Abstracts"
} |
Effect of weight loss on ventricular repolarization in normotensive severely obese patients with and without heart failure.
Obesity has been reported to be associated with delayed ventricular repolarization. The purpose of this study was to assess ventricular repolarization in normotensive severely obese subjects with and without heart failure (HF) and to assess the effect of weight loss on ventricular repolarization in such patients. Twenty-eight patients with and 39 patients without HF (body mass index ≥ 40 kg/m(2)) were studied before and after weight loss from bariatric surgery. Corrected QT interval (QTc) was measured on 12-lead electrocardiograms using Bazett's formula. QTc dispersion was calculated by subtracting the minimum from the maximum QTc on each 12-lead electrocardiogram. Electrocardiograms and transthoracic echocardiograms were performed preoperatively and at the nadir of postoperative weight loss. Mean QTc and QTc dispersion were significantly longer/greater in subjects with HF than in those without HF (P < 0.0001). Weight loss produced significant reductions in mean QTc and QTc dispersion in both subgroups (P < 0.0001). Pre-weight loss left ventricular (LV) mass/height and presence or absence of HF independently predicted pre-weight loss QTc and QTc dispersion (P < 0.0001). Weight loss-induced decrease in LV mass/height independently predicted weight loss-induced decreases in QTc and QTc dispersion (P < 0.0001). HF independently predicts QTc and QTc dispersion in normotensive severely obese patients. Decrease in the LV mass resulting from weight loss independently predicts reduction in QTc and QTc dispersion in such patients. | {
"pile_set_name": "PubMed Abstracts"
} |
Mitochondrial dysfunction promotes cell migration via reactive oxygen species-enhanced β5-integrin expression in human gastric cancer SC-M1 cells.
Mitochondrial dysfunction has been shown to promote cancer cell migration. However, molecular mechanism by which mitochondrial dysfunction enhances gastric cancer (GC) cell migration remains unclear. Mitochondria specific inhibitors, oligomycin and antimycin A, were used to induce mitochondrial dysfunction and to enhance cell migration of human gastric cancer SC-M1 cells. Antioxidant N-acetylcysteine (NAC) was used for evaluating the effect of reactive oxygen species (ROS). Protein expressions of epithelial-to-mesenchymal transition (EMT) markers and the cell-extracellular matrix (ECM) adhesion molecules, the integrin family, were analyzed. A migratory subpopulation of SC-M1 cells (SC-M1-3rd) was selected using a transwell assay for examining the association of mitochondrial bioenergetic function, intracellular ROS content and β5-integrin expression. Clinicopathologic characteristics of β5-integrin expression were analyzed in GC specimens by immunohistochemical staining. Treatments with mitochondrial inhibitors elevated mitochondria-generated ROS and cell migration of SC-M1 cells. The protein expression of β5-integrin and cell surface expression of αvβ5-integrin were upregulated, and which were suppressed by NAC. Pretreatments with NAC and anti-αvβ5-integrin neutralizing antibody respectively prevented the mitochondrial dysfunction-induced cell migration. The selected migratory SC-M1-3rd cells showed impaired mitochondrial function, higher mitochondria-generated ROS, and increased β5-integrin expression. The migration ability was also repressed by anti-αvβ5-integrin neutralizing antibody. In clinical specimens, GCs with higher β5-integrin protein expression had more aggressive behavior. In conclusion, mitochondrial dysfunction may lead to GC progression by enhancing migration through mitochondria-generated ROS mediated β5-integrin expression. These results support the role of mitochondrial dysfunction in GC progression. | {
"pile_set_name": "PubMed Abstracts"
} |
A retrospective comparison of 2 methods of intraoperative margin evaluation during partial nephrectomy.
Intraoperative pathological consultation is often used to achieve negative margins during partial nephrectomy. Commonly a tumor bed biopsy for frozen section is taken from the most suspicious area of the defect. Alternatively the pathologist may perform prosection of the intact partial nephrectomy specimen and prepare frozen sections of suspicious areas. We determined the sensitivity and specificity of these 2 methods and a combined method. Records of 251 cases performed at a single institution between 2005 and 2007 were retrospectively analyzed. Of the patients 56% were male. Mean age was 58.8 years. Laparoscopic resection was performed in 76% of cases. Mean greatest tumor dimension was 2.9 cm. Tumor bed biopsy was done in 120 cases, of which 15 (12.5%) showed positive final margins. With permanent section as the gold standard, tumor bed biopsy was 25% sensitive (95% CI 6-46) and 100% specific (95% CI 96-100) for detecting positive margins. In contrast, gross intraoperative consultation with or without frozen section in 163 cases, including 112 with gross intraoperative consultation only and 51 with frozen section, revealed positive final margins in 16 (9.8%) and was 75% sensitive (95% CI 50-90) and 100% specific (95% CI 97-100). The combined method involving tumor bed biopsy plus gross intraoperative consultation was 100% sensitive (95% CI 60-100) and 100% specific (95% CI 89-100). The data support the routine practice of combined gross pathological consultation and tumor bed biopsy. When the combined method is not used, gross intraoperative consultation is more diagnostically accurate than tumor bed biopsy. The data do not support the common practice of examining the tumor bed biopsy alone. | {
"pile_set_name": "PubMed Abstracts"
} |
Complications of jejunoileal bypass for morbid obesity.
Fifty-two patients had jejunoileal bypass surgery. End-to-end (Scott) or end-to-side (Payne) shunts were randomly selected for each patient; 31 standard length shunts and 21 shortened bypasses were performed. Only 22 patients had an acceptable result, whereas 30 patients had inadequate weight loss (less than 2.3 kg [5 lb] per month per year) or had gastrointestinal tract, metabolic, or surgical complications judged severe enough to render the outcome less than adequate. There was one death, and four patients required reanastomosis of the bypass. The primary deteriminant of success was age, ie, younger patients had clearly better results than older patients. In general, shorter shunts produced more weight loss than standard bypass procedures, but were associated with an increased complication rate. Three new complications of jejunolieal bypass are reported: acute comonic dilation with necrosis, beriberi, and lupus erythematosus. | {
"pile_set_name": "PubMed Abstracts"
} |
Deciphering metabolic responses of biosurfactant lichenysin on biosynthesis of poly-γ-glutamic acid.
Poly-γ-glutamic acid (γ-PGA) is an extracellularly produced biodegradable polymer, which has been widely used as agricultural fertilizer, mineral fortifier, cosmetic moisturizer, and drug carrier. This study firstly discovered that lichenysin, as a biosurfactant, showed the capability to enhance γ-PGA production in Bacillus licheniformis. The exogenous addition of lichenysin improved the γ-PGA yield up to 17.9% and 21.9%, respectively, in the native strain B. licheniformis WX-02 and the lichenysin-deficient strain B. licheniformis WX02-ΔlchAC. The capability of intracellular biosynthesis of lichenysin was positively correlated with γ-PGA production. The yield of γ-PGA increased by 25.1% in the lichenysin-enhanced strain B. licheniformis WX02-Psrflch and decreased by 12.2% in the lichenysin-deficient strain WX02-ΔlchAC. Analysis of key enzyme activities and gene expression in the TCA cycle, precursor glutamate synthesis, and γ-PGA synthesis pathway revealed that the existence of lichenysin led to increased γ-PGA via shifting the carbon flux in the TCA cycle towards glutamate and γ-PGA biosynthetic pathways, minimizing by-product formation, and facilitating the uptake of extracellular substrates and the polymerization of glutamate to γ-PGA. Insight into the mechanisms of enhanced production of γ-PGA by lichenysin would define the essential parameters involved in γ-PGA biosynthesis and provide the basis for large-scale production of γ-PGA. | {
"pile_set_name": "PubMed Abstracts"
} |
Long-term ground penetrating radar monitoring of a small volume DNAPL release in a natural groundwater flow field.
An earlier field experiment at Canadian Forces Base Borden by Brewster and Annan [Geophysics 59 (1994) 1211] clearly demonstrated the capability of ground penetrating radar (GPR) reflection profiling to detect and monitor the formation of DNAPL layers in the subsurface. Their experiment involved a large volume release (770 L) of tetrachloroethylene into a portion of the sand aquifer that was hydraulically isolated from groundwater flow by sheet pile walls. In this study, we evaluated the ability of GPR profiling to detect and monitor much smaller volume releases (50 L). No subsurface confining structure was used in this experiment; hence, the DNAPL impacted zone was subjected to the natural groundwater flow regime. This condition allowed us to geophysically monitor the DNAPL mass loss over a 66 month period. Reflectivity variations on the GPR profiles were used to infer the presence and evolution of the solvent layers. GPR imaging found significant reflectivity increases due to solvent layer formation during the two week period immediately after the release. These results demonstrated the capacity of GPR profiling for the detection and monitoring of lesser volume DNAPL releases that are more representative of small-scale industrial spills. The GPR imaged solvent layers subsequently reduced in both areal extent and reflectivity after 29 months and almost completely disappeared by the end of the 66 month monitoring period. Total DNAPL mass estimates based on GPR profiling data indicated that the solvent mass was reduced to 34%-36% of its maximum value after 29 months; only 4%-9% of the solvent mass remained in the study area after 66 months. These results are consistent with independent hydrogeological estimates of remaining DNAPL mass based on the downgradient monitoring of the dissolved solvent phase. Hence, we have concluded that the long-term GPR reflectivity changes of the DNAPL layers are likely the result from the dissolution of chlorinated solvents residing in those layers. The long-term monitoring results demonstrated that GPR profiling is a promising non-invasive method for use at DNAPL contaminated sites in sandy aquifers where temporal information about immiscible contaminant mass depletion due to either natural flow or remediation is needed. However, our results also indicated that the GPR signature of older DNAPL impacted zones may not differ greatly from the uncontaminated background if significant mass reduction due to dissolution has occurred. | {
"pile_set_name": "PubMed Abstracts"
} |
The changing perinatal and maternal outcome in chorioamnionitis.
Chorioamnionitis is difficult to detect clinically, but its recognition in those at risk is essential. A retrospective study of 140 patients from among 26,129 deliveries was conducted over a 2-year period. The findings suggest that in modern obstetric practice, both perinatal and maternal complications associated with chorioamnionitis (particularly sepsis) are infrequent problems. Four neonatal deaths occurred but no infants died of sepsis. There were no maternal deaths, but 38 patients developed postpartum infections. Cesarean section did not appear to improve either perinatal or maternal outcome. With the use of appropriate modern antibiotics, extraperitoneal cesarean section and cesarean hysterectomy are probably no longer indicated. Not all neonates born out of a microbiologically contaminated intrauterine environment required antibiotic therapy, however, and individualization is recommended. | {
"pile_set_name": "PubMed Abstracts"
} |
SFPQ/PSF-TFE3 renal cell carcinoma: a clinicopathologic study emphasizing extended morphology and reviewing the differences between SFPQ-TFE3 RCC and the corresponding mesenchymal neoplasm despite an identical gene fusion.
Xp11 translocation renal cell carcinoma (RCC) with SFPQ/PSF-TFE3 gene fusion is a rare epithelial tumor. Of note, the appearance of the gene fusion does not necessarily mean that it is renal cell carcinoma. The corresponding mesenchymal neoplasms, including Xp11 neoplasm with melanocytic differentiation, TFE3 rearrangement-associated perivascular epithelioid cell tumor (PEComa) and melanotic Xp11 translocation renal cancer, can also harbor the identical gene fusion. However, the differences between Xp11 translocation RCC and the corresponding mesenchymal neoplasm have only recently been described. Herein, we examined 5 additional cases of SFPQ-TFE3 RCCs using clinicopathologic, immunohistochemical, and molecular analyses. One tumor had the typical morphologic features of SFPQ-TFE3 RCC, whereas other 3 cases demonstrated the unusual morphologic features associated with pseudorosettes formation or clusters of smaller cells, mimicking TFEB RCC. The remaining one showed branching tubules and papillary structure composed of clear and eosinophilic tumor cells. Immunohistochemically, all 5 cases demonstrated moderate (2+) or strong (3+) positive staining for TFE3, PAX-8 and CD10, whereas no cases demonstrated TFEB, Cathepsin K, CA-IX, CK7, Melan-A, or HMB-45 expression. Genetically, the fusion transcripts were identified in 3 cases by reverse-transcription polymerase chain reaction (RT-PCR). On the basis of fluorescence in situ hybridization (FISH) analysis, all the cases were detected with SFPQ-TFE3 gene fusion. Clinical follow-up data were available for all the patients, and no one developed tumor recurrence, progression, or metastasis. We also review the differences between SFPQ-TFE3 RCC and the corresponding mesenchymal neoplasm despite the identical gene fusion. The presence of pseudorosettes also expands the known histological features of SFPQ-TFE3 RCC. | {
"pile_set_name": "PubMed Abstracts"
} |
Infection after primary anatomic versus primary reverse total shoulder arthroplasty.
Deep periprosthetic infection is a serious complication after total shoulder arthroplasty (TSA) and is associated with suboptimal clinical results. This retrospective study aimed to determine whether a difference in infection rate existed after primary anatomic TSA (aTSA) and primary reverse TSA (rTSA). A TSA database was retrospectively reviewed to identify primary TSAs performed from 2004 to 2012 and isolate cases of confirmed deep periprosthetic infection. Data collected included age, sex, preoperative diagnosis, previous nonarthroplasty operative history, implant type, and infecting organism. From 814 primary TSAs performed, deep periprosthetic infections were confirmed in 16 shoulders. Infections occurred in 6 aTSAs and 10 rTSAs, with no significant difference among the prosthesis types. Pathogens included Staphylococcus spp, Propionibacterium acnes, and Escherichia coli. In the subgroup of shoulders with no previous operations, no significant difference in infection rate was found among the prosthesis types. Shoulders with previous nonarthroplasty operations undergoing primary TSA exhibited a significantly higher (P = .016) infection rate compared with shoulders with no operative history. Both aTSA and rTSA performed in previously operated-on shoulders demonstrated higher infection rates compared with shoulders with no prior operative intervention. The infection rate was significantly higher (P = .016) in the rTSA group. There is no difference in infection rate after primary aTSA and primary rTSA in shoulders that have not undergone previous operative interventions. Infection is more likely to develop in shoulders undergoing primary rTSA that have had one or more nonarthroplasty operative procedures. | {
"pile_set_name": "PubMed Abstracts"
} |
Analysis of CASP12 diagnostic and prognostic values in cervical cancer based on TCGA database.
The present study aims to find a differential protein-coding gene caspase 12 (CASP12) in cervical cancer (CC) based on the (TCGA) database and verify its clinical diagnostic and prognostic values. The transcriptome and clinicopathological data of CC were downloaded from the TCGA database and through screening, we found that PDE2A and CASP12 were independent prognostic factors for CC patients. According to the median expression, the patients were divided into groups with high and low CASP12 and PDE2A expression. There was no difference in survival between PDE2A high and low expression groups (P=0.099), whereas there was a significant difference between CASP12 high and low expression groups (P=0.033). The serum from 68 CC patients (experimental group) and 50 healthy people (control group) was collected to detect the relative expression of CASP12 using qRT-PCR and plotted the ROC curve. The relative expression of CASP12 in the experimental group was significantly lower than in the control group (P<0.05). The area under the curve (AUC) of CASP12 was 0.865. There were statistically significant differences between CASP12 groups with high and low expression in terms of differentiation, lymph node metastasis, tumor size, FIGO staging, and clinical outcomes (P<0.05), but not in terms of age, HPV types and pathological types (P>0.05). The 3-year survival in the CASP12 low expression group was significantly worse than in the CASP12 high expression group (P=0.028). In conclusion, the expression level of CASP12 can be used as a diagnostic and prognostic biomarker for patients with CC. | {
"pile_set_name": "PubMed Abstracts"
} |
Local high-capacity adenovirus-mediated mCTLA4Ig and mCD40Ig expression prolongs recombinant gene expression in skeletal muscle.
Multiple forms of muscular dystrophy are due to the absence of cytoskeletal muscle proteins that normally protect the integrity of muscle cells. The lack of any adequate treatments for these devastating diseases propels research toward the development of strategies for gene delivery to skeletal muscle. High-capacity adenoviral vectors (HC-AdV) devoid of all viral coding sequences have been developed to avoid expression of viral proteins by the gene therapy vector. However, the capsid proteins that are an essential component of the input viral vector and any residual helper virus in the vector preparation could induce an immune response. Furthermore, the therapeutic protein provided by a gene transfer vector presents the potential to induce an immune response in a patient who does not express a normal cellular protein due to genetic mutation. Therefore, we hypothesize that some immune suppression will be required with therapeutic gene delivery designed for the treatment of patients with inherited muscle diseases. In this study, we constructed and rescued three HC-AdVs expressing murine CTLA4Ig, murine CD40Ig, or both. The backbone vector without a gene insert was rescued as a negative control vector. The production of relevant proteins from each vector was determined in vitro. In vivo function of each of the immunosuppressant vectors was assayed by co-injection with an enhanced green fluorescent protein (EGFP)-expressing first-generation adenoviral vector (AdEGFP) into the tibialis anterior muscle of C57BL/10 mice. Higher levels of muscle EGFP expression were observed in animals receiving an immunosuppressant vector. Furthermore, the production of total anti-AdV and anti-EGFP antibodies was reduced in mice treated with each of the three immunosuppressant vectors. A second intramuscular administration of AdEGFP alone 4 weeks after the initial co-injection was successful in all immunosuppressant vector-treated groups, but not in the negative control vector-treated group. All groups had a high antibody response to adenoviral proteins after the second injection of AdEGFP alone, indicating that the initial co-injection did not tolerize against vector capsid antigens. | {
"pile_set_name": "PubMed Abstracts"
} |
Sequence and gene organization of 24 circles from the Cotesia plutellae bracovirus genome.
Twenty-four genomic segments of Cotesia plutellae bracovirus (CpBV) were completely sequenced, and their genomic structures were analyzed. The aggregated genome size is 351,299 bp long and exhibits an average GC content of approximately 34.6%. Average coding density is about 32.3%, and 125 putative open reading frames (ORFs) are predicted. More than half (52.5%) of predicted genes are annotated as hypothetical, but they share sequence similarities with those of other bracoviral genomes. The annotated ORFs can be classified into the known bracoviral families, in which a family of protein tyrosine phosphatase is the largest, including 36 ORFs, suggesting a significant role during parasitization. In addition, 8 and 7 ORFs encode ankyrin-like and EP1-like genes, respectively. Some predicted genes are known only in Cotesia-associated bracoviral genomes. Phylogenetic analyses based on PTP, ankyrin and EP1-like gene groups revealed no correlation between bracoviruses. | {
"pile_set_name": "PubMed Abstracts"
} |
Monoclonal antibodies to human melanoma-associated antigens: an amplified enzyme-linked immunosorbent assay for the detection of antigen, antibody, and immune complexes.
An amplified, indirect biotin-avidin micro-enzyme-linked immunosorbent assay was developed for the measurement of human melanoma-associated antigens, either free or circulating with associated immunoglobulin in patient sera. Parameters and specificity of detection were assessed using monoclonal antibody to human melanoma-associated antigens. The main advantages of the assay are its flexibility, through the use of indirect detection and a variety of formats, and its sensitivity, with a lower limit of antibody detection at 100 pg/well and a lower limit of soluble antigen detection at 10 pg/well. The assay was applied to cell surface antigen detection with monoclonal antibody 9.2.27 to a melanoma-associated antigen against a panel of glutaraldehyde-fixed cells, and gave similar binding specificity as assessed by a previous 125I-Protein A assay. Utilizing a unique "sandwich" format, aMr 100,000 melanoma-carcinoma-associated antigen was quantitated in melanoma patient sera and found highly elevated in Stage IV disease. The same sandwich format was also used to detect and determine the class of human immunoglobulin associated with circulating Mr 100,000 human melanoma-associated antigens in normal donor sera. Thus, the sensitivity and flexibility of this enzyme-linked immunosorbent assay system make it particularly suitable for numerous applications in the study of monoclonal antibody-defined tumor-associated antigens. | {
"pile_set_name": "PubMed Abstracts"
} |
Permutation test following covariate-adaptive randomization in randomized controlled trials.
In randomized controlled trials, patients are recruited and randomly allocated to treatments. Patients are never randomly sampled from large population of patients on treatments under study. Therefore, it is important to consider the design and statistical analysis based on the randomization model. In this article, we show theoretically that a permutation test based on the difference in means is identical to analysis of covariance if marginal covariate balance is completely attained. Our theoretical results and Monte Carlo simulation study suggest that the permutation test following Pocock-Simon's covariate-adaptive randomization can be a useful alternative to traditional population-based tests in a confirmatory randomized controlled trial with important prognostic factors. The proposed procedure is illustrated with modified data from the randomized placebo-controlled trial of pirfenidone. | {
"pile_set_name": "PubMed Abstracts"
} |
Formation of a ligand-binding site for the acetylcholine receptor in vitro.
Investigation of the mechanisms by which the subunits of ligand-gated ion channels fold and associate to form oligomers has been hampered by the lack of an in vitro system in which these reactions occur. We have established conditions in a rabbit reticulocyte translation system supplemented with canine pancreatic microsomes under which the alpha and delta subunits of the nicotinic acetylcholine receptor (AChR) fold and assemble to form a heterodimer with a cholinergic binding site comparable with that found in the intact AChR. Folding of the alpha subunit was followed by its ability to bind alpha-bungarotoxin. Folding efficiency was highly sensitive to changes in the redox potential of the translation medium and was favored by an oxidizing environment. Acquisition of the toxin binding conformation required N-linked core glycosylation but not oligosaccharide trimming, suggesting that oligosaccharide-dependent interaction of chaperones with the alpha subunit is not essential for correct subunit folding. The conformationally mature alpha subunit specifically associated with the delta subunit but not the beta subunit to form a heterodimer with a high affinity ligand-binding site. These data demonstrate, for the first time, correct folding and assembly of the AChR subunits in an in vitro system. | {
"pile_set_name": "PubMed Abstracts"
} |
Molecular cloning and expression patterns of Cu/Zn-superoxide dismutases in developing soybean seeds.
Assuming that the amount of superoxide radicals generated in vivo correlates with the production of ergastic substances such as storage proteins, the coordinated response of detoxication enzymes such as superoxide dismutases is largely exploited to understand the self-defense systems of plant. Here we examined expression of the genes for superoxide dismutases during seed development of soybean. The cDNAs encoding a cytosolic copper/zinc form and an iron form of the above enzyme have been cloned and then employed as probes, separately. Northern blotting results suggested that both superoxide dismutase mRNAs are expressed at the maximum level, preceding a developmental stage when mRNA encoding glycinin, soybean 11S-storage protein, at the maximum. | {
"pile_set_name": "PubMed Abstracts"
} |
Early cessation of triptorelin in in vitro fertilization: a double-blind, randomized study.
To compare the efficacy of two early cessation protocols of triptorelin treatment in controlled ovarian hyperstimulation with the conventional long protocol in in vitro fertilization/intracytoplasmic sperm injection. A double-blind, randomized, multicenter study. Three Dutch hospitals. One hundred seventy-eight women randomized to one of three treatment groups at the start of stimulation. Midluteally started triptorelin administration was continued until the first day of hMG treatment (group S), or up to and including the fourth day of hMG treatment (group M) or the day of hCG injection (group L). Occurrence of a premature LH surge. One premature LH surge was observed in group M but not in groups S and L. Both early cessation protocols (S and M) are at least as effective as the long protocol (L) with regard to the number of oocytes (11.1 and 10.3 vs. 9.3), number of embryos (7.3 and 6.5 vs. 5.5), and ongoing pregnancy rate (28% and 24% vs. 21%). Early cessation of triptorelin on day 1 of hMG treatment in a midluteally started IVF protocol is as effective as the traditional long protocol in preventing a premature LH surge and results in similar fertility effects. | {
"pile_set_name": "PubMed Abstracts"
} |
Refractive surgery for unilateral high myopia in children.
To evaluate the safety and efficacy of refractive surgery in children. Pediatric patients with unilateral high myopia who were 9 years of age or older were offered refractive surgery to supplement optical correction. The patients and families were informed that the operation may not improve their best-corrected visual acuity. Photorefractive keratectomy (PRK) or laser in situ keratomileusis (LASIK) was performed on the more myopic eye with the use of topical anesthesia. Cycloplegic refraction, stereopsis, motility, and best-corrected visual acuity were measured before the procedure and at 2 months and 20 months after the procedure. All patients had completed amblyopia therapy before surgery. Fourteen eyes of 14 patients aged 9 to 14 years received refractive surgery. Average age at the time of surgery was 11.9 years (+/-1,6). Average corrected preoperative visual acuity was 20/147 (+/-0.065 in decimals). Average preoperative refraction was -7.96 D (+/-2,16) spherical equivalent. Twenty months after refractive surgery, the uncorrected visual acuity averaged 20/129 (+/-0.08 in decimals) and best-corrected vision averaged 20/121 (+/-0.08 in decimals). Average refraction was -0.46 D (+/-0,58) at 2 months and -0.67 D (+/-0,68) D at 20 months. An average myopic shift in refraction of -0.22 D was found in treated eyes during the 20 months of follow-up; this was not statistically significant (P =.69). Three patients had LASIK and 11 patients had PRK. LASIK patients averaged -0.875 D of myopic shift over 20 months of follow-up. Those with PRK averaged -0.025 D. This difference was not statistically significant (P =.10). The vision of 5 of 14 patients improved 1 or 2 lines after refractive surgery. Two patients who had 20/80 vision preoperatively improved to 20/60. No patients lost any lines of vision. Only 4 patients demonstrated stereopsis preoperatively, and all retained stereopsis postoperatively. No patient gained stereopsis. LASIK and PRK can be performed safely and effectively in children who are cooperative enough to undergo the procedures with topical anesthesia. Refractive surgery does not improve vision in densely amblyopic eyes but may give modest improvement in those that are mildly amblyopic. No significant complications were encountered aside from a myopic shift over time. | {
"pile_set_name": "PubMed Abstracts"
} |
Transgenic over-expression of a motor protein at high levels results in severe cardiac pathology.
Transgenesis has become a useful tool in effecting a complete or partial remodeling of the cardiac contractile apparatus. Although gene dosage effects were initially a concern, recent data showed that the heart is able to accommodate varying levels of transgenic over-expression without detectable ill effects. The present study was designed to test the limits of the transgenic paradigm in terms of the production of a cardiac phenotype due simply to the over-expression of a contractile protein. To this end, eight lines of mice which express an isoform of the essential myosin light chain 1 that is normally found in the adult ventricle (ELC1v) were generated. Overt phenotype was correlated both with the level of expression/protein replacement and copy number of the transgene. Two of the lines showed essentially complete replacement of the atrial isoform (ELC1a) with ELC1v. However, the phenotypes of the two lines differed dramatically. The line with the lower copy number (37 copies), and moderate over-expression (16 fold) showed no overt pathology while a line with very high copy number (94 copies) and extremely high levels of over-expression (27-50 fold) developed a significant atrial hypertrophy, dilation and cardiomyopathy. These data indicate that very high expression levels of a contractile protein can cause a cardiac pathology that is unrelated to its degree of replacement in the sarcomere and the unique role(s) it may assume in motor protein function. | {
"pile_set_name": "PubMed Abstracts"
} |
Controlled release of paracetamol from amylodextrin tablets: in vitro and in vivo results.
Amylodextrin is a suitable excipient for the design of solid controlled-release systems. The release of paracetamol from tablets containing 30% drug and 70% amylodextrin was studied in vitro and in vivo. In vitro dissolution profiles showed almost-constant drug release rates during 8 hr, when measured in 0.05 M buffer, pH 6.8. Peroral administration of the tablets to man showed almost-constant paracetamol plasma levels up to 14 hr, as compared to fast absorption and fast elimination of a reference paracetamol solution. The plasma profiles of eight volunteers demonstrated a small intersubject variability during the first day after tablet administration. Increasing variability and decreasing plasma levels during the second day were caused by excretion of tablets from the bodies. Cumulative input as a function of time showed near-zero-order drug release during the first day. The in vivo results indicate that amylodextrin tablets are not hydrolyzed by alpha-amylase, present in the gastrointestinal tract. | {
"pile_set_name": "PubMed Abstracts"
} |
Foreign language learning difficulties in Italian children: are they associated with other learning difficulties?
A group of seventh- and eighth-grade Italian students with low achievement (LA) in learning English as a foreign language (FL) was selected and compared to a group with high achievement (HA) in FL learning. The two groups were matched for age and nonverbal intelligence. Two experiments were conducted to examine the participants' verbal and nonverbal learning skills, such as native language reading accuracy, speed and comprehension, calculation, and attention and self-regulation. Both experiments showed that the LA group seemed at risk for reading comprehension difficulties, but its reading speed and accuracy were within the average range according to Italian norms. The results also excluded the possibility that FL learning difficulties of LA participants could be associated with a deficit in calculation. Furthermore, according to teachers' ratings, children with LA appeared at risk for attention-deficit disorder (ADD). The pattern of learning difficulties of seventh- and eighth-grade participants with LA appeared to be not completely comparable with that of high school students at risk of FL learning difficulties as described in the literature. | {
"pile_set_name": "PubMed Abstracts"
} |
Electroencephalographic assessment of pneumatically powered penetrating and non-penetrating captive-bolt stunning of bulls.
The electroencephalographic (EEG) responses of 31 bulls (zebu crossbred cattle) stunned with either pneumatically powered Jarvis penetrating (PCB) or non-penetrating captive bolt (NPCB) was examined. Animals were organized into two treatment groups: PCB (n = 20); and NPCB (n = 11) stunning, both using airline pressures of 220 psi (1517 kPa). All bulls shot with PCB (n = 20/20) had patterns of EEG activity that were inconsistent with consciousness. Of the cattle shot with NPCB 82% (n = 9/11) showed waveforms suggesting complete unconsciousness. After stunning two NPCB bulls had periods of normal EEG activity and maintenance (Ptot, delta, theta, beta) or increased (alpha) spectral power compared to pre-treatment values, indicating incomplete concussion. The study showed that pneumatic PCB stunning was effective in rendering all bulls unconscious, while NPCB was less effective. This highlights the potential animal welfare risks associated with NPCB compared to PCB stunning of mature bulls in commercial abattoirs. | {
"pile_set_name": "PubMed Abstracts"
} |
The effect of hypo-allergenic formulas in infants at risk of allergic disease.
Development of atopic disease seems to depend on both genetic factors and exposure to several environmental factors. At present ther is evidence that the mode of early infant feeding influences the development of food allergy, whereas daily exposure to inhalant allergens and daily exposure to tobacco smoke is found to be associated with an increased risk of recurrent wheezing/asthma and inhalant allergy. In infants with atopic predisposition (first-degree relatives), exclusively breastfeeding > or = four months is found associated with a significant reduction of the cumulative prevalence of cow's milk allergy/intolerance (CMA/CMI) during the first 1-2 years of age. When breastmilk is insufficient or lacking a substitute formula is needed. Several recent prospective studies show a preventative effect of extensively hydrolysed formula (eHF) in combination with avoidance of cow's milk proteins and solid foods during > or = 4 months in high-risk infants on the cumulative prevalence of food allergy and atopic dermatitis during the first 2-4 years of life. Partially hydrolysed formulas (pHF) may be effective in allergy prevention, but due to drawbacks of study design and lack of documentation pHF cannot be recommended at present. The results of studies comparing the preventive effect of eHF and pHF are awaited. The protective effect on the development of cow's milk allergy is a real prevention and not only a postponement of the onset of symptoms. No studies have demonstrated a preventive effect of dietary measures as regards asthma/inhalant allergy, at present until the age of four years. As no studies concerning the preventive effect of avoidance of milk and other foods after the age of 4-6 months of life have been performed, recommendation of preventive elimination diets beyond this age is empirically based. In order to reduce the costs, to minimize the risk of stigmatisation and the risk of malnutrition it is important to avoid unnecessary restrictive and prolonged diets. A diet period of 4-6 months seems sufficient in most infants. At present eHF are recommended for avoidance of cow's milk. Some high risk infants may benefit from maternal diet during lactation, but there is no documented beneficial effect of maternal diet during pregnancy. | {
"pile_set_name": "PubMed Abstracts"
} |
Removal of Cd(II) from aqueous solution by a composite hydrogel based on attapulgite.
Chitosan-g-poly(acrylic acid)/attapulgite composite with 30 wt% attapulgite content was used to remove Cd(II) from aqueous solutions. The parameters influencing the adsorption were investigated. When Cd(CH3COO)2 was used as the solute of the solution, the equilibrium adsorption capacity was evidently larger than that of the other three cadmium salts (Cd(NO3)2, CdCl2, CdSO4). Results from kinetic experiments showed that the rate of Cd(II) adsorption on the composite was quite fast, more than 90% of Cd(II) adsorption occurred within the initial 3 min, and the adsorption equilibrium may be reached within 10 min. The adsorption process can be well described by the pseudo-second-order kinetic model. The equilibrium adsorption isotherm was fitted for not only the Langmuir model but also the Freundlich model. Fourier transform infrared spectra before and after adsorption of Cd(II) on the composite showed that complexation was considered as the main mechanism. To evaluate the reuse value of the composite, a consecutive adsorption-desorption process was performed five times, and the results implied that the composite has the potential for regeneration and reuse. | {
"pile_set_name": "PubMed Abstracts"
} |
An impression and cast construction technique for implant-retained auricular prostheses.
Implant-retained auricular prostheses present a challenge with regard to the esthetic placement of the margins of the prosthesis. A technique for the impression and master cast fabrication to produce an implant-retained auricular prosthesis is described. This technique allows control of the esthetic margin, fabrication of a durable master cast with planned blockout, and inclusion of a replica of the retentive bar, which allows processing duplicate prostheses without returning the retentive bar to the mold. | {
"pile_set_name": "PubMed Abstracts"
} |
Combined azaperone and metomidate anaesthesia in liver transplantation in the pig.
Combined azaperone and metomidate anaesthesia has been used in 86 surgical procedures on 84 piglets, either as such or deepened and prolonged. 51 animals were sacrificed at the end of the procedure as planned before. The anesthesia allowed the performance of various short and long operations. Out of the 33 remaining pigs, submitted to 35 operations, 4 died during the procedure of a technical fault; 2 did not recover from a deep hypothermia (below 10 degrees C); 1 died from the hepatic coma induced through the operative procedure. The other 26 awoke and recovered spontaneous breathing within 1-4 h following the type of anaesthesia and operating procedure they had submitted, which dured from 15 to 330 min. | {
"pile_set_name": "PubMed Abstracts"
} |
Reduced postprandial heat production with gavage as compared with meal feeding in human subjects.
The postprandial changes in resting metabolic rate (RMR) were measured in eight subjects after the ingestion of 735 kcal taken in the form of a meal or fed by stomach tube. A much larger increase in RMR was found with meal feeding (MF) as compared with tube feeding (TF). Measurements of respiratory quotient (RQ) indicated that the increase in RMR with MF is related to increased glucose oxidation, whereas with TF it is possibly explained primarily by the cost of energy storing. Determination of plasma norepinephrine (NE) indicated that the sympathetic nervous system is activated by MF but not by TF. Similarly, plasma glucose and insulin determinations have shown that the secretion of insulin is increased during food ingestion but only with the MF situation. Thus in the 30 min after the beginning of the meal, an increased glucose oxidation was observed that is possibly related to an enhanced NE and insulin secretion; such effects were nonexistent in the TF situation. These results would not substantiate the notion of the specific dynamic action of food and would indicate that independent of the composition of the ingested nutrients, the postprandial increase in RMR is also influenced by sensory and cognitive stimulations. | {
"pile_set_name": "PubMed Abstracts"
} |
Tandem Mass Spectrometry Analysis Using the ThermoFinnigan LCQ System.
INTRODUCTIONThis protocol provides guidance for setting the parameters for a typical data-dependent MS/MS acquisition analysis using the ThermoFinnigan LCQ system. The method consists of a continual cycle beginning with one scan of MS (scan one), which records all of the m/z values of the ions present at that moment in the gradient, followed by two rounds of MS/MS. The initial MS/MS scan is of the first most-intense ion recorded from the MS scan. The second MS/MS scan is of the second most-intense ion recorded from scan one. Dynamic exclusion is activated to improve the protein identification capacity during the analysis. | {
"pile_set_name": "PubMed Abstracts"
} |
Intra-aortic balloon counterpulsation for cardiogenic shock due to cardiac contusion in an elderly trauma patient.
Blunt thoracic trauma may cause cardiac contusion and cardiogenic shock resistant to inotropic support. The use of intra-aortic balloon counterpulsation (IABCP) as a mechanical means of augmenting cardiac function following cardiac contusion is rare with case reports largely limited to its use in young trauma patients. We describe the case of a frail, 80-year-old woman who suffered cardiac contusion in a motor vehicle crash. She developed cardiogenic shock with electrocardiograph changes, elevated troponin T and severe global dysfunction on echocardiography. She was successfully managed with invasive monitoring, inotropic support and IABCP. This case provides support for aggressive resuscitation even in the very elderly as recovery from severe cardiac contusion may be possible. | {
"pile_set_name": "PubMed Abstracts"
} |
Expression of Fas/FasL in CD8+ T and CD3+ Foxp3+ Treg cells--relationship with apoptosis of circulating CD8+ T cells in hepatocellular carcinoma patients.
Dysfunction of the host immune system in cancer patients can be due to a number of factors, including lymphocyte apoptosis. Several studies showed that Foxp3+T cells take part in inducing this process by expressing FasL in tumor patients. However, the relationship between apoptosis, CD8+T cells and Foxp3+T cells in HCC patients is still unclear. The present study was designed to investigate the correlation between apoptosis levels and Fas/FasL expression in CD8+T lymphocytes and Foxp3+T cells in patients with HCC. CD8+T cells and CD3+Foxp3+T cells were tested from peripheral blood of HCC patients and normal controls and subjected to multicolor flow cytometry. The expression of an apoptosis marker (annexin V) and the death receptor Fas in CD8+T cells and FasL in CD3+Foxp3+T cells were evaluated. Serum TGF-β1 levels in patients with HCC were measured by enzyme-linked immunosorbent assay. The relationship between apoptosis and Fas expression, as well as FasL expression in CD3+Foxp3+T cells was then evaluated. The frequency of CD8+T cells binding annexin V and Fas expression in CD8+T cells, were all higher in HCC patients than normal controls and the proportion of apoptotic CD8+T cells correlated with their Fas expression. Serum TGF-β1 levels correlated inversely with CD3+Foxp3+T cells. Fas/FasL interactions might lead to excessive turnover of CD8+T cells and reduce anti-tumor immune responses in patients with HCC. Further investigations of apoptosis induction in Fas+CD8+T cells in vitro are required. | {
"pile_set_name": "PubMed Abstracts"
} |
Transmission via the face is one route of methicillin-resistant Staphylococcus aureus cross-infection within a hospital.
It is generally accepted that hospital personnel must not touch their faces, and indeed must not elevate their hands above the shoulder in the ward, because many bacteria including Staphylococcus aureus colonize the face. However, possible methicillin-resistant Staphylococcus aureus (MRSA) cross-infection by way of faces has not yet been properly examined. One hundred and seventy-eight isolates from 159 inpatients from February 1999 to January 2000 were subjected to chromosomal DNA analysis by pulsed-field gel electrophoresis (PFGE). The 178 MRSA isolates were classified into 43 PFGE types. Cross-infection was more frequent in the Department of Neurology [average number of patients per PFGE pattern (n/PFGE) = 4.0] than in the other six nonsurgical departments (n/PFGE = 1.0-1.6), and more frequent in four surgical departments (n/PFGE = 2.0-4.5) than in the other eight (n/PFGE = 1.0-1.7). In neurology, patients' faces were more often touched for examination of the cranial nerves than in the other departments. In the above four surgical departments, organs in the face were chiefly operated upon, and the patients' faces were also touched for care before and after the operation. Our study reveals the possibility of MRSA being transmitted by way of patients' faces in a hospital. | {
"pile_set_name": "PubMed Abstracts"
} |
Plasma IFN-γ-inducible chemokines CXCL9 and CXCL10 correlate with survival and chemotherapeutic efficacy in advanced pancreatic ductal adenocarcinoma.
Recent studies have suggested that the CXCL9, 10, 11/CXCR3 axis is significant in immune regulation and therapeutic efficacy in human cancers; however, its role in pancreatic ductal adenocarcinoma (PDAC) remains unknown. This study serves to evaluate the prognostic prediction value of plasma IFN-γ-inducible chemokines, CXCL9 and CXCL10, in advanced PDAC. Two hundred patients with advanced PDAC receiving palliative chemotherapy were retrospectively recruited. The association between Plasma CXCL9/CXCL10 levels and survival time was first analyzed in a test group of 110 patients and then confirmed in a validation group of 90 patients. High levels of CXCL9 and CXCL10 were significantly correlated with longer overall survival (OS) in advanced PDAC patients (314 vs. 136 days for CXCL9, P < 0.0001, and 374 vs. 163 days for CXCL10, P < 0.0001, respectively) in the test group, which was consistent with the results derived from the validation group. In addition, high levels of CXCL9 and CXCL10 were associated with longer time to progression (TTP) in patients receiving chemotherapy (100 vs. 60 days for CXCL9, P = 0.0021, and 104 vs. 67 days for CXCL10, P = 0.0057, respectively). Multivariate analyses confirmed that CXCL9 and CXCL10 were independent prognostic predictors for OS (hazard ratio [HR]: 0.452, P < 0.001 for CXCL9; and HR: 0.586, P = 0.007 for CXCL10, respectively) and TTP (HR: 0.656, P = 0.015 for CXCL9; and HR: 0.687, P = 0.040 for CXCL10, respectively). Plasma CXCL9 and CXCL10 can be used to predict survival of advanced PDAC patients receiving chemotherapy, allowing clinicians to potentially improve treatment outcomes by identifying candidates for aggressive therapy. | {
"pile_set_name": "PubMed Abstracts"
} |
Development and evaluation of a primary care interprofessional education intervention to support people with dementia.
International policy documents emphasize the need to develop interprofessional education (IPE) to support collaborative dementia care in the community. The aim of this short report is to describe the development and pilot evaluation of an interprofessional dementia education workshop for primary care healthcare professionals. A three-hour workshop was iteratively developed through consultation with an expert reference group and people with dementia. The workshop was piloted with three separate primary care teams. A total of fifty-four primary care based healthcare staff who represented fourteen different health care roles in primary care participated in the pilot evaluation. The pilot workshops were evaluated using a mixed method approach which included post-workshop questionnaires for participants (n= 54)and a post-workshop focus group (n=8) with the program design team and workshop facilitators. The results of the pilot phase indicated that the workshop was useful and feasible. The workshop improved participants' self-reported knowledge, understanding and confidence to support people with dementia and their families.Areas for improvement were identified and will be used to inform improvements to the workshop content and delivery in advance of a national roll-out. Future evaluations of the implementation of this interprofessional educational workshop will focus on its impact on healthcare professional behavior and outcomes for the person with dementia and their families. | {
"pile_set_name": "PubMed Abstracts"
} |
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