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The liver in AIDS.
Deficits in cell-mediated immunity in AIDS result in a wide variety of hepatic complications, including granulomas, cytomegalovirus hepatitis, multimicrobial AIDS cholangiopathy, Kaposi's sarcoma, and lymphoma. Kupffer cells are the major hepatic target cell population for human immunodeficiency virus-1 (HIV-1), and rhesus monkeys with simian immunodeficiency virus infection have served as a model for ultrastructural analysis of viral clearance by these cells. The majority of patients with established AIDS reveal abnormalities on serum liver tests. In these individuals, the differential diagnosis includes opportunistic infections and neoplasms, as well as possible concomitant chronic viral hepatitis B, C, D, and G, and drug hepatotoxicity. This article reviews the spectrum of hepatic pathology in AIDS and discusses the effects of HIV-1 infection on hepatitis virus infections. | {
"pile_set_name": "PubMed Abstracts"
} |
Gastric feeding and "gut rousing" in acute pancreatitis.
The "pancreatic rest" concept is entrenched in the management of acute pancreatitis. As a result, "nonstimulatory" feeding has been widely advocated in patients with this disease, being parenteral nutrition 2-3 decades ago and jejunal tube feeding in the past decade. However, accumulating clinical evidence from the fields of acute pancreatitis and critical care medicine suggests that gastric feeding is as safe and effective as jejunal feeding in most patients. This has paved the way for a new conceptual framework called "gut rousing." Acute pancreatitis management now requires consideration of gut function. Enteral nutrition is a gut-directed therapy, and avoiding stimulation of the pancreas in patients with acute pancreatitis need not overshadow the main goal of maintaining or restoring gut function. | {
"pile_set_name": "PubMed Abstracts"
} |
Polyelectrolyte multilayers of poly (l-lysine) and hyaluronic acid on nanostructured surfaces affect stem cell response.
Laser interference lithography (LIL) and the layer-by-layer (LbL) technique are combined here for the first time to design a system with variable nanotopographies and surface viscoelasticity to regulate cell behavior. LIL is used to generate hexagonally arranged nanostructures of gold with different periodicity. In contrast, LBL is used to assemble a multilayer system of poly-l-lysine and hyaluronic acid on top of the nanostructures. Moreover, the viscoelastic properties of that system are controlled by chemical cross-linking. We show that the topography designed with LIL is still present after multilayer deposition and that the formation of the multilayer system renders the surfaces hydrophilic, which is opposite to the hydrophobic nature of pristine nanostructures. The heterogenic system is applied to study the effect on adhesion and differentiation of human adipose-derived stem cells (hADSC). We show that hADSC spreading is increasing with cross-linking degree on flat multilayers, while it is decreasing on nanostructures modified with multilayers. In addition, early effects on signal transduction processes are seen. Finally, hADSC differentiation into chondrogenic and osteogenic lineages is superior to adipogenic lineages on nanostructures modified with multilayers. Hence, the presented system offers great potential to guide stem cell differentiation on surfaces of implants and tissue engineering scaffolds. | {
"pile_set_name": "PubMed Abstracts"
} |
Geriatric assessment with focus on instrument selectivity for outcomes.
More than one half of all cancers in developed countries occur in patients aged 70 years and older. Therefore, in recent years, there has been a growing interest in integrating a comprehensive geriatric assessment into the management of these patients with cancer. This review article emphasizes the data gathered so far on the correlation between such an assessment and outcome in patients with cancer. The most developed data relate to functional status and comorbidity. Geriatric instruments appear more sensitive than classic oncological instruments in measuring functional status. There is also good evidence that as a patient's age advances, comorbidity affects in an increasing fashion their survival and cancer management. Some evidence is beginning to appear as to the impact a comprehensive geriatric assessment could have on the oncologic management of older patients with cancer. | {
"pile_set_name": "PubMed Abstracts"
} |
Dynamic topomerization of Cu(I)-complexed pseudorotaxanes.
Dynamic molecular motions resulting from the folding of a flexible macrocyclic component in a Cu(I)-complexed pseudorotaxane have been evidenced by variable temperature NMR experiments. The proposed conformational changes are also supported by the X-ray crystal structures of the compounds and computational studies. | {
"pile_set_name": "PubMed Abstracts"
} |
The North West Diabetic Pregnancy Audit: a practical system for multi-centre diabetic pregnancy audit.
Improving care for women with pre-gestational diabetic pregnancy is a core objective of the St Vincent Declaration and the Diabetes National Service Framework. The aim was to develop a practicable collaborative audit methodology for pre-gestational diabetic pregnancy. In 1999, care professionals in the north-west of England agreed standards and a simple monthly data collection system. Annual reports are compiled to summarize compliance with the standards. Each hospital receives an individualized report comprising tables and funnel plots that allow between-hospital comparisons. Data on pre-gestational diabetic pregnancies are collated from 30 maternity units. Funnel plots and tables presented in the annual reports highlight any large differences between hospitals in booking and outcome measures for diabetic pregnancies. The annual audit reports allow the assessment of current management and outcomes for diabetic pregnancies at a regional and local level. These reports help to identify areas where diabetic pregnancy care requires further attention. | {
"pile_set_name": "PubMed Abstracts"
} |
[Surgical treatment of rheumatoid polyarthritis].
Surgical treatment of rheumatoid arthritis aims to suppress pain, correct deformities, and restore function. This treatment should be adapted to each patient on the basis of clinical and radiographic criteria, with priority given to surgical treatment of the lower limbs. In the early stages, conservative surgery is called for (tenosynovectomy, joint synovectomy) and in later stages, palliative surgery (arthrodesis, prosthesis). | {
"pile_set_name": "PubMed Abstracts"
} |
Monoclonal antibodies directed against human myoglobin: characterization and application in a bideterminant radioimmunoassay.
Monoclonal hybridoma cell lines secreting antibodies directed against human myoglobin were selected. Two of these cell lines were grown in mouse ascitic fluid resulting in the production of large quantities of antibody. Antimyoglobin antibodies isolated from the ascitic fluids were employed in the development of the sensitive solid-phase, bideterminant radioimmunoassay for human myoglobin that uniquely recognizes two different epitopes on the same molecule. | {
"pile_set_name": "PubMed Abstracts"
} |
[Cytological characteristics of the human periodontal fibroblasts].
This review examines current concepts of functional morphology and molecular biology of periodontal fibroblasts. These cells appear to be the major cellular elements of human periodontium that realize its functions. Recent evidence on fibroblast structure, regeneration, synthetic and secretory activity, adhesion to various matrix components, migration, response to hormones, cytokines, growth factors and microbial products as well as on their interaction with other cellular elements of periodontium, is reviewed. On the basis of the newest findings on cellular and molecular biology, the concept of the central role of fibroblasts in the local network of cytokine regulation that controls the integrated function of periodontal cells, is formulated. | {
"pile_set_name": "PubMed Abstracts"
} |
The pill: perceptions and usage among Nigerian students.
The knowledge and use of oral contraception were investigated in 498 students from 5 tertiary institutions in south-eastern Nigeria. Awareness of the pill was high (92.2%) but usage was comparatively low (17.5%). Post-coital oral contraception was more often used (11.5%) than the other types. Perceptions about the risks of the pill were variable: 46.4% of the students believed that it could damage the uterus, and 41% said it could cause infertility; 31.7% believed that it caused amenorrhea, while only 14.9% of the students admitted ignorance of the risks of the pill. There was a lack of desire to use the pill in 72.3% of the respondents. Only 18.7% desired to do so, including students already using the pill. The most common source of information on contraception was peer groups (43.6%), while lectures and sex instructions constituted the least common source (11.2%). Poor information on the pill and ignorance were identified as the major factors militating against pill usage. Accordingly, the role of early and adequate sex and contraceptive education in improving pill usage in this population and developing countries in general is highlighted. | {
"pile_set_name": "PubMed Abstracts"
} |
The effect of adenotonsillectomy on ventilatory control in children with obstructive sleep apnea.
The contribution of ventilatory control to the pathogenesis of obstructive sleep apnea (OSA) in children and the effect of adenotonsillectomy are unknown. We aimed to examine the difference in ventilatory control between children with OSA and those without OSA. We also examined the effect of adenotonsillectomy on parameters of ventilatory control. Healthy children with OSA and matched controls were recruited. Polysomnography was performed before adenotonsillectomy in the OSA group and 6 months postoperatively. Controls underwent the same assessment at the two time points. Loop gain (LG), controller gain (CG), and plant gain (PG), which reflect the stability of ventilatory control, chemoreceptor sensitivity and the pulmonary control of blood gas in response to a change in ventilation, respectively, were estimated from polysomnographic tracings which included spontaneous sighs and tracings with tidal breathing. A linear mixed model was used to examine the changes of the ventilatory control parameters from baseline to 6 months. Ninety-nine children aged 7-13 were recruited to the study. Fifty-three with OSA and 46 controls. At baseline, compared with controls, children with OSA had higher PG and lower CG. LG did not differ between groups. Six months following adenotonsillectomy, there was a significant decrease in PG in the OSA group, while no change observed in the control group. The study demonstrates that the pulmonary control of blood gas homeostasis is disturbed in children with OSA and it normalizes following adenotonsillectomy. | {
"pile_set_name": "PubMed Abstracts"
} |
Per3, a circadian gene, is required for Chk2 activation in human cells.
PER3 is a member of the PERIOD genes, but does not play essential roles in the circadian clock. Depletion of Per3 by siRNA almost completely abolished activation of checkpoint kinase 2 (Chk2) after inducing DNA damage in human cells. In addition, Per3 physically interacted with ATM and Chk2. Per3 overexpression induced Chk2 activation in the absence of exogenous DNA damage, and this activation depended on ATM. Per3 overexpression also led to the inhibition of cell proliferation and apoptotic cell death. These combined results suggest that Per3 is a checkpoint protein that plays important roles in checkpoint activation, cell proliferation and apoptosis. | {
"pile_set_name": "PubMed Abstracts"
} |
Histone deacetylase inhibition modulates deoxyribonucleotide pools and enhances the antitumor effects of the ribonucleotide reductase inhibitor 3'-C-methyladenosine in leukaemia cells.
Histone deacetylase (HDAC) inhibitors are a new class of epigenetic agents that were reported to enhance the cytotoxic effects of classical anticancer drugs through multiple mechanisms. However, which of the possible drug combinations would be the most effective and clinically useful are to be determined. We treated the HL60 and NB4 promyelocytic leukaemia cells with a combination of the ribonucleotide reductase (RR) inhibitor 3'-C-methyladenosine (3'-Me-Ado) and several hydroxamic acid-derived HDAC inhibitors, including two recently synthesized molecules, MC1864 and MC1879, and the reference compound trichostatin A (TSA). The results showed significant growth inhibitory and apoptotic synergistic effects with the combinations. Hence, we evaluated the effects of the combinations on cell cycle distribution and on the level of several proteins involved in the apoptotic process (p21, caspase-3, Bcl-2, Bax, AIF). Since HDAC inhibitors increased the G1-S transition block induced by 3'-Me-Ado, an effect on RR activity was hypothesized. Indeed, the HPLC evaluation of intracellular deoxyribonucleotide (dNTP) pools showed that both TSA and MC1864 induced a decrease in dNTPs, even if with a somewhat different pattern, suggesting that RR inhibition contributes to the observed synergism. Furthermore, while TSA was shown to activate the intrinsic apoptotic pathway, MC1864 induced a dose-dependent increase in ROS and AIF levels. Moreover, the treatment with the radical scavenger N-acetylcysteine determined a significant inhibition of MC1864- but not TSA-mediated synergistic effects. Hence, our findings are consistent with a possible role of HDAC inhibitor mediated-ROS induction in RR inhibition and in the potentiation of RR inhibitor-mediated apoptosis. | {
"pile_set_name": "PubMed Abstracts"
} |
Electrochromic and colorimetric properties of nickel(II) oxide thin films prepared by aerosol-assisted chemical vapor deposition.
Aerosol-assisted chemical vapor deposition (AACVD) was used for the first time in the preparation of thin-film electrochromic nickel(II) oxide (NiO). The as-deposited films were cubic NiO, with an octahedral-like grain structure, and an optical band gap that decreased from 3.61 to 3.48 eV on increase in film thickness (in the range 500-1000 nm). On oxidative voltammetric cycling in aqueous KOH (0.1 mol dm(-3)) electrolyte, the morphology gradually changed to an open porous NiO structure. The electrochromic properties of the films were investigated as a function of film thickness, following 50, 100, and 500 conditioning oxidative voltammetric cycles in aqueous KOH (0.1 mol dm(-3)). Light modulation of the films increased with the number of conditioning cycles. The maximum coloration efficiency (CE) for the NiO (transmissive light green, the "bleached" state) to NiOOH (deep brown, the colored state) electrochromic process was found to be 56.3 cm(2) C(-1) (at 450 nm) for films prepared by AACVD for 15 min followed by 100 "bleached"-to-colored conditioning oxidative voltammetric cycles. Electrochromic response times were <10 s and generally longer for the coloration than the bleaching process. The films showed good stability when tested for up to 10 000 color/bleach cycles. Using the CIE (Commission Internationale de l'Eclairage) system of colorimetry the color stimuli of the electrochromic NiO films and the changes that take place on reversibly oxidatively switching to the NiOOH form were calculated from in situ visible spectra recorded under electrochemical control. Reversible changes in the hue and saturation occur on oxidation of the NiO (transmissive light green) form to the NiOOH (deep brown) form, as shown by the track of the CIE 1931 xy chromaticity coordinates. As the NiO film is oxidized, a sharp decrease in luminance was observed. CIELAB L*a*b* coordinates were also used to quantify the electrochromic color states. A combination of a low L* and positive a* and b* values quantified the perceived deep brown colored state. | {
"pile_set_name": "PubMed Abstracts"
} |
Statistical considerations for confirmatory clinical trials for similar biotherapeutic products.
For the purpose of comparing the efficacy and safety of a Similar Biotherapeutic Product (SBP) to a Reference Biotherapeutic Product (RBP), the "Guidelines on Evaluation of Similar Biotherapeutic Products (SBPs)" issued by the World Health Organisation (WHO), states that equivalence or non-inferiority studies may be acceptable. While in principle, equivalence trials are preferred, non-inferiority trials may be considered if appropriately justified, such as for a medicinal product with a wide safety margin. However, the statistical issues involved in the design, conduct, analysis and interpretation of equivalence and non-inferiority trials are complex and subtle, and require that all aspects of these trials be given careful consideration. These issues are important in order to ensure that equivalence and non-inferiority trials provide valid data that are necessary to draw reliable conclusions regarding the clinical similarity of an SBP to an RBP. | {
"pile_set_name": "PubMed Abstracts"
} |
Intratetrad mating and the evolution of linkage relationships.
Mating among the immediate products of meiosis (intratetrad mating) is a common feature of many organisms with parthenogenesis or with mating-type determination in the haploid phase. Using a three-locus deterministic model we show that intratetrad mating, unlike other systems of mating, allows sheltering of deleterious recessive alleles even if there is only partial linkage between a mating locus and a load locus. Moreover, modifiers that reduce recombination between the load and mating-type locus will spread to fixation, even when there is no linkage disequilibrium between these loci in the population as a whole. This seeming contradiction to classical expectation is because partial linkage generates linkage disequilibrium among segregating loci within a tetrad, which then acts as the "mating unit." | {
"pile_set_name": "PubMed Abstracts"
} |
Synthesis and Characterization of a Series of Monometallo-, Bimetallo-, and Heterobimetallo-1,2-Ethene-Linked Cofacial Bisporphyrins.
The stepwise synthesis and characterization of a family of selectively metalated 1,2-bis[5-(2,3,7,8,12,13,17,18-octaethylporphyrinyl)]ethenes is described. These compounds are distinct from most of the previously reported bisporphyrin systems which possessed rigid linking groups or multiple strapping units, and their syntheses offer several advantages over the previously prepared systems. Among the members of this family we report the first heterobimetallic bisporphyrins able to exist as either cis (cofacial) or trans (extended) isomers including cis 13 {[Ni(II)][Zn(II)]}, cis 14{[Ni(II)][Cu(II)]}, and trans 15{[Cu(II)][Mn(III)]}. Products were characterized by (1)H NMR spectroscopy (except those containing Cu(II) and/or Mn(III)), visible spectroscopy, mass spectrometry, and elemental analyses. X-ray structural data are provided for bisporphyrins 4, 10, and 13-15: cis 4 (C(74)H(88)N(8)Co(2)), monoclinic, space group P2(1)/c, a = 21.097(15) Å, b = 14.120(5) Å, c = 22.987(11) Å, beta = 108.59(4) degrees, Z = 4; cis 10 (C(74)H(90)N(8)Cu) monoclinic, space group P2(1)/n, a = 19.695(5) Å, b = 14.427(3) Å, c = 24.679(6) Å, beta = 112.72(2) degrees, Z = 4; cis 13 (C(74)H(88)N(8)NiZn) monoclinic, space group P2(1)/c, a = 21.187(3) Å, b = 13.9091(11) Å, c = 22.682(3) Å, beta = 108.674(10) degrees, Z = 4; cis 14 (C(74)H(88)N(8)NiCu) monoclinic, space group P2(1)/c, a = 21.169(3) Å, b = 13.875(2) Å, c = 22.660(3) Å, beta = 108.64(9) degrees, Z = 4; trans 15 (C(74)H(88)N(8)CuMnCl), triclinic, space group P&onemacr;, a = 9.8123(13), b = 13.742(3), c = 14.774(3), alpha = 83.26(2), beta = 78.532(12), gamma = 78.347(14), Z = 1. | {
"pile_set_name": "PubMed Abstracts"
} |
Effects of patient demands on satisfaction with Japanese hospital care.
The objective of this study was to detect whether there was any difference among the characteristics of patient satisfaction between two patient emphasis groups: patients demanding technical elements of hospital care and patients demanding interpersonal elements. The sample for this study was drawn from in-patients discharged from 77 voluntarily participating hospitals throughout Japan. The relationship between overall satisfaction with hospital care and patient satisfaction, and the evaluation of a hospital's reputation, was explored by stepwise multiple regression analysis of 33 variables relevant to aspects of hospital care for each patient group. In the interpersonal emphasis (IE) group, 'nurse's kindness and warmth' was associated significantly with overall satisfaction, while 'skill of nursing care' and 'nurse's explanation' were significant predictors of overall satisfaction in the technical emphasis (TE) group. On the other hand, 'doctor's clinical competence', 'recovery from distress and anxiety', and items pertaining to the hospital's reputation were significantly related to overall satisfaction in both emphasis groups. For overall patient satisfaction, it is essential to satisfy specific items related to the aspect of hospital care emphasized by the patient. Specific significant predictors of overall satisfaction (e.g. 'doctor's clinical competence') were indispensable measures of professional performance in hospital care, irrespective of the patients' emphasis. A positive perception of hospital reputation items might increase overall patient satisfaction with Japanese hospitals. | {
"pile_set_name": "PubMed Abstracts"
} |
Exhuming the body: revisiting the role of the visible body in ethnographic research.
Looking back is often painful. Although in the first instance I might approach a research project with enthusiasm and confidence, in retrospect the sins of omission can become very obvious. In reviewing five research projects employing qualitative ethnographic methods, I explored the underacknowledged role of the visible body in these projects. Although the task of retrospection is blessed with the accumulated wisdom of others, I emphasize the importance of ongoing self-scrutiny and critical analysis of methodological dogmas for researchers and their practices. | {
"pile_set_name": "PubMed Abstracts"
} |
Local re-inversion coronary MR angiography: arterial spin-labeling without the need for subtraction.
To implement a double-inversion bright-blood coronary MR angiography sequence using a cylindrical re-inversion prepulse for selective visualization of the coronary arteries. Local re-inversion bright-blood magnetization preparation was implemented using a nonselective inversion followed by a cylindrical aortic re-inversion prepulse. After an inversion delay that allows for in-flow of the labeled blood-pool into the coronary arteries, three-dimensional radial steady-state free-precession (SSFP) imaging (repetition/echo time, 7.2/3.6 ms; flip angle, 120 degrees, 16 profiles per RR interval; field of view, 360 mm; matrix, 512, twelve 3-mm slices) is performed. Coronary MR angiography was performed in three healthy volunteers and in one patient on a commercial 1.5 Tesla whole-body MR System. In all subjects, coronary arteries were selectively visualized with positive contrast. In addition, a middle-grade stenosis of the proximal right coronary artery was seen in one patient. A novel T1 contrast-enhancement strategy is presented for selective visualization of the coronary arteries without extrinsic contrast medium application. In comparison to former arterial spin-labeling schemes, the proposed magnetization preparation obviates the need for a second data set and subtraction. | {
"pile_set_name": "PubMed Abstracts"
} |
Frequency gradients of the basic electrical and mechanical rhythms along the rat intestine.
The basic mechanical rhythm (BMR), initiated and maintained by the basic electrical rhythm (BER), ensures the muscular tonus of the intestine during the interdigestive phases. The frequency and amplitude gradient for the BER waves and, correlated with them, the frequency and size gradient of the BMR contraction force have an anatomic determination. The circular muscle layer is discontinuous, consisting of cylindrical segments separated by conjunctive tissue, segments whose size increases starting from the duodenum to the ileocecal valve. The electrical connection between the cylindrical segments formed by the circular muscles is provided by the layer of longitudinal muscle fibres. Each and every segment generates a general voltage which is higher than that of the preceeding segment, so that the slow BER wave becomes amplified along the intestine. In correlation with the electrical phenomena, the BMR contractile waves will become more ample in the terminal region of the intestine, facilitating the progress through peristalsis of the luminal content with greater viscosity. | {
"pile_set_name": "PubMed Abstracts"
} |
Toxicologic and immunologic evaluations of N-(all-trans-retinoyl)-DL-leucine and N-(all-trans-retinoyl)glycine.
Sprague-Dawley rats were dosed by gavage daily for 28 days with 5, 15, or 50 mg/kg of N-(all-trans-retinoyl)-DL-leucine (RL), N-(all-trans-retinoyl)glycine (RG), or all-trans-retinoic acid (RA). On the basis of mortality incidence, fracture incidence, body weight, and histopathologic effects, RG was slightly to moderately less toxic than RA, and RL was significantly less toxic than RA or RG. Doses that had no effect on weight loss and produced no bone fractures were approximately 5 and 15 mg/kg/day for RA administered to males or females, respectively; greater than 15 mg/kg/day for RG administered to males or females; and greater than 50 mg/kg/day for RL administered to males or females. At these doses, RA and RG produced effects, detectable at the microscopic level, of lymphoid hyperplasia and hematopoietic cell proliferation in the spleen, lymphoid hyperplasia in lymph nodes, necrosis of the cortex of the thymus, hypertrophy of the zona fasciculata of the adrenal, a periportal pattern of cytoplasmic vacuolization in hepatocytes, hematopoietic cell proliferation in the liver, epithelial hyperplasia and subacute inflammation in the forestomach, and osteodystrophy. Serological alterations consisted of reduced serum albumin levels and elevated levels of triglycerides and alkaline phosphatase. For RL, similar microscopic effects, dependent on dose level and sex, were observed in spleen, thymus, adrenal, and liver. In vitro, RL was as active as RA in potentiating pokeweed mitogen-induced lymphocyte proliferation; RG was inactive. This study indicates that, relative to RA and RG, RL has less toxicity but similar immunological effects. Since RL and RG expressed little or no binding affinity for cellular RA-binding protein, the immunological effects of these retinoids may be expressed by mechanisms not linked to this protein. | {
"pile_set_name": "PubMed Abstracts"
} |
[Stratified analysis of the relationship between traditional Chinese medicine constitutional types and health status in the general population based on data of 8,448 cases].
To examine the relationship between traditional Chinese medicine (TCM) constitutional types and health status among groups of different age or gender in the general population of China. Data of 8 448 cases were randomly sampled from a database of 21 948 cases of a cross-sectional survey on the TCM constitutional types and health status which was carried out in 9 provinces or municipalities of China (Jiangsu, Anhui, Gansu, Qinghai, Fujian, Beijing, Jilin, Jiangxi and Henan) according to gender and age structure of the Chinese population in 2005. Scores of health-related quality of life scale--the Medical Outcomes Study 36-Item Short-Form Health Survey (MOS SF-36)--were analyzed by Nemenyi test to compare the health status of individuals with different constitutional types. Compared with the gentleness type, the MOS SF-36 scores of the 8 types of pathological constitution were significantly low (P<0.05) among groups of different age or gender. The MOS SF-36 score was the lowest in men of the qi-deficiency, qi-depression and blood-stasis types, while it was the lowest in women of the phlegm-dampness, qi-depression and qi-deficiency types. For the age group of 15 to 34, the special diathesis, qi-depression and blood-stasis types had the lowest MOS SF-36 scores; for the age group of 35 to 59, the qi-deficiency, qi-depression and blood-stasis types had the lowest MOS SF-36 scores; for the age group of over 60, the qi-deficiency, qi-depression and phlegm-dampness types had the lowest MOS SF-36 scores. In groups of different gender or age, the MOS SF-36 scores of the 8 types of pathological constitution were significantly lower than that of the gentleness type, indicating a deficient health status. The health status of different types of constitution showed different characteristics in groups of different gender or age. | {
"pile_set_name": "PubMed Abstracts"
} |
Hypothalamic dysfunction without hamartomas causing gelastic seizures in optic nerve hypoplasia.
This report describes gelastic seizures in patients with optic nerve hypoplasia and hypothalamic dysfunction without hypothalamic hamartoma. All participants (n = 4) from the optic nerve hypoplasia registry study at Children's Hospital Los Angeles presenting with gelastic seizures were included. The clinical and pathology characteristics include hypothalamic dysgenesis and dysfunction, but no hamartomas. Optic nerve hypoplasia is the only reported condition with gelastic seizures without hypothalamic hamartomas, suggesting that hypothalamic disorganization alone can cause gelastic seizures. | {
"pile_set_name": "PubMed Abstracts"
} |
[Age-and, education-corrected number connection test and digit symbol test in diagnosis of minimal hepatic encephalopathy].
To determine the age-and education-corrected control values for the number connection test (NCT) and digit symbol test (DST) psychometric measures to increase their accuracy for diagnosis of minimal hepatic encephalopathy (MHE). The NCT Part A (NCT-A) and DST were administered to 843 healthy volunteers (age range:16-65 years; education:more than 1 year) and 429 patients with liver cirrhosis (with Child-Pugh classification of liver function). The normal values were defined as the mean ± 2 standard deviations (2SD);MHE was defined by abnormal results on at least one psychometric test. The statistical significance of differences in MHE diagnosis according to the various control values (age and education-corrected or not) was assessed by the chi-square test and logistic regression analysis. NCT-A and DST were found to be influenced by age (standard coefficient 0.405, P =0.000 and standard coefficient-0.527, P =0.000 respectively) and education (standard coefficient-0.347, P =0.000 and standard coefficient 0.405, P =0.000 respectively). Among the 120 patients with liver cirrhosis who were diagnosed with MHE (27.97%), 113 had abnormal NCT-A results (26.34%), 54 had abnormal DST results (12.59%),and 47 had abnormal results on both tests (10.96%). Among these 120 MHE-positive patients, 21 were classified as Child-Pugh A (19.81%), 46 as Child-Pugh B (23.71%), and 53 as Child-Pugh C (41.09%);the MHE-positive rate was significantly greater in the patients with Child-Pugh C classification than those with either Child-Pugh B or Child-Pugh A (P < 0.01). Logistic analysis showed that when the control data was corrected for age and education, the MHE diagnosis was related with liver function (P =0.000), regardless of age (P =0.328) and education (P =0.563). When the control data was uncorrected, the MHE diagnosis was not only influenced by liver function (P =0.000) but also by age (P =0.000) and education (P =0.005). Age and education-corrected control values can increase the accuracy of MHE diagnosis by NCT-A and DST. | {
"pile_set_name": "PubMed Abstracts"
} |
The Stanley Foundation Bipolar Treatment Outcome Network. I. Longitudinal methodology.
The NIMH-Stanley Foundation Bipolar Treatment Outcome Network, a multisite clinical trials network, has been established to address many of the neglected areas of research in bipolar illness. The Network was designed so that it would be able to conduct randomized clinical trials at several different levels of methodologic rigor (blinded and open-label) both in academic and community practice settings in order to better assess long-term efficacy of existing treatments and develop new ones. In this fashion, large numbers of representative patients with bipolar disorder have been enrolled with an additional focus of elucidating possible clinical and biological predictors of treatment response. The unique focus of the Network is its systematic longitudinal approach to illness so that patients can be assessed comprehensively over the long-term in sequential randomized clinical trials at critical clinical decision points where data on relative efficacy are inadequate. Bipolar I and bipolar II patients with a range of illness variants and comorbidities are included. Daily prospective ratings of severity of mania and depression and associated degree of functional impairment are completed on the NIMH-Life Chart Method and a modified Clinical Global Impressions Scale for Bipolar Illness (CGI-BP) is utilized. More detailed cross-sectional ratings for depression (Inventory of Depressive Symptomatology), mania (Young Mania Rating Scale), and psychosis (Positive and Negative Syndrome Scale) are additionally used at academic centers. This article describes the rationale for the Network, its guiding principles, methods, and study design to systematically assess the highly variable course of bipolar illness and its response to current and future treatments. | {
"pile_set_name": "PubMed Abstracts"
} |
Potentially inappropriate medication prescribed to elderly outpatients at a general medicine unit.
To establish the prevalence of potentially inappropriate medications prescribed for elderly patients, to identify the most commonly involved drugs, and to investigate whether age, sex and number of medications were related with the prescription of these drugs. Prescriptions for 1,800 elderly patients (≥ 60 years) were gathered from a database. These prescriptions were written by general physicians at a tertiary level university hospital in the city of Sao Paulo, Brazil, from February to May 2008. Only one prescription per patient was considered. The prescriptions were classified according to sex and age (60-69, 70-79 and ≥ 80). The Beers criteria (2003 version) were used to evaluate potentially inappropriate medications. Most of the sample comprised women (66.6%) with a mean age of 71.3 years. The mean prevalence of potentially inappropriate medication prescriptions was 37.6%. The 60-69 age group presented the highest prevalence (49.9%). The most frequently prescribed potentially inappropriate medications to women were carisoprodol, amitriptyline, and fluoxetine; amitriptyline, carisoprodol, fluoxetine and clonidine were prescribed more often to men. The female sex (p<0.001; OR=2.0) and number of medications prescribed (p<0.001) were associated with prescription of potentially inappropriate medications. The chance of having a prescription of these drugs was lower among patients aged over 80 years (OR=0.7). The mean number of prescribed medications for both sexes and all age groups was 7.1. The mean number of medications per patient was higher among females (p<0.001); this result was not age-dependent (p=0.285). The prevalence of potentially inappropriate medications was similar to previously reported values in the literature and was correlated with the female sex. The chance of having a potentially inappropriate medication prescription was lower among patients aged over 80 years. The chance of having a potentially inappropriate medications prescription increased proportionally with the number of medications prescribed (≥ 5). | {
"pile_set_name": "PubMed Abstracts"
} |
The frequency of papillomavirus infection in cervical precancerous lesions in Japan: an immunoperoxidase study.
In order to establish the frequency of human papillomavirus (HPV) infection in cervical precancerous lesions of Japanese patients, cervical materials routinely biopsied in the past year were examined immunohistochemically for the papillomavirus genus-specific antigen. Of a total of 832 cervical biopsy specimens, 46 (5.5%) were immunohistochemically positive for HPV. In this study, 206 patients were diagnosed as having dysplasia or carcinoma in situ (CIS), and HPV antigen was found in 15% of these patients. It was found in 13% of patients with mild dysplasia, 28% of those with moderate dysplasia, 17% of those with severe dysplasia and 4.5% of those with CIS. However, HPV antigen was detected only in the epithelium with dysplastic change, not in cancerous areas. | {
"pile_set_name": "PubMed Abstracts"
} |
Comparison of the new injury severity score and the injury severity score in multiple trauma patients.
To assess whether these characteristics of less misclassification and greater area under receiver operator characteristic (ROC) curve of the new injury severity score (NISS) are better than the injury severity score (ISS) as applying it to our multiple trauma patients registered into the emergency intensive care unit (EICU). This was a retrospective review of registry data from 2 286 multiple trauma patients consecutively registered into the EICU from January 1,1997 to December 31, 2006 in the Second Affiliated Hospital, Medical School of Zhejiang University in China. Comparisons between ISS and NISS were made using misclassification rates, ROC curve analysis, and the H-L statistics by univariate and multivariate logistic progression model. Among the 2 286 patients, 176 (7.7%) were excluded because of deaths on arrival or patients less than 16 years of age. The study population therefore comprised 2 110 patients. Mean EICU length of stay (LOS) was 7.8 days ?2.4 days. Compared with the blunt injury group, the penetrating injury group had a higher percentage of male, lower mean EICU LOS and age. The most frequently injured body regions were extremities and head/neck, followed by thorax, face and abdomen in the blunt injury group; whereas, thorax and abdomen were more frequently seen in the penetrating injury group. The minimum misclassification rate for NISS was slightly less than ISS in all groups (4.01% versus 4.49%). However, NISS had more tendency to misclassify in the penetrating injury group. This, we noted, was attributed mainly to a higher false-positive rate (21.04% versus 15.55% for ISS, t equal to 3.310, P less than 0.001), resulting in an overall misclassification rate of 23.57% for NISS versus 18.79% for ISS (t equal to 3.290, P less than 0.001). In the whole sample, NISS presented equivalent discrimination (area under ROC curve: NISS equal to 0.938 versus ISS equal to 0.943). The H-L statistics showed poorer calibration (48.64 versus 32.11, t equal to 3.305, P less than 0.001) in the penetrating injury group. NISS should not replace ISS because they share similar accuracy and calibration in predicting multiple blunt trauma patients. NISS may be more sensitive but less specific than ISS in predicting mortality in certain penetrating injury patients. | {
"pile_set_name": "PubMed Abstracts"
} |
The elaboration of the 'Stockholm convention' on persistent organic pollutants (POPs): a negotiation process fraught with obstacles and opportunities.
The conclusion in December 2000 of the negotiations for the 'Stockholm Convention' can clearly be labeled as a success. The Convention text was negotiated in merely five sessions of the Intergovernmental Negotiating Committee (INC) and accomplished after its fifth session despite the fact that numerous controversial issues, such as the inclusion of new substances under the ambit of the Convention, the acknowledgement of the precautionary principle or--clearly most controversial--the financing mechanisms, remained to be resolved. This paper attempts to provide a somewhat impressionistic account of the negotiations leading to the conclusion of the 'Stockholm Convention' as experienced by the members of the Swiss delegation participating in the negotiations of the INC. Besides a brief overview on the 'history' of the negotiations, it will focus on some issues of special interest--and controversy--to the negotiators, and finally attempt to provide an outlook on the future of the work performed by the INC and the implementation of the Convention. Issues of special interest are environmental policy issues, capacity building and financing, trade-related issues, precautionary principles, and technical and scientific issues. | {
"pile_set_name": "PubMed Abstracts"
} |
Autopsy findings after coronary rotational atherectomy.
We describe the findings at autopsy in a patient who underwent Rotablator atherectomy of the right coronary artery. During the procedure, the artery became occluded. Despite attempts to reopen the vessel with balloon angioplasty and emergency coronary artery bypass grafting, the patient developed irreversible cardiac failure and expired 2 days after the Rotablator procedure. At autopsy, the right coronary artery was found to be occluded by thrombus. No evidence of dissection or perforation of the vessel wall was seen. Small intramyocardial arteries and arterioles, downstream from the treated vessel, were embolized by pulverized atheroma. | {
"pile_set_name": "PubMed Abstracts"
} |
Cloning and characterization of an essential Saccharomyces cerevisiae gene, TAF40, which encodes yTAFII40, an RNA polymerase II-specific TATA-binding protein-associated factor.
In this report we describe the cloning and initial characterization of TAF40, a gene that encodes a yeast TATA-binding protein-associated factor (yTAF) of Mr = approximately 40,000. This gene has many similarities to other yTAFs described thus far in that it is present at a single copy per haploid genome, it is essential for viability, and the deduced protein sequence of yTAF40 exhibits similarity to previously described human and Drosophila TAFIIs. Immunological studies confirm that yTAF40 protein is a subunit of a large multiprotein TATA-binding protein-TAF complex that contains a subset of the total number of the yTAFs present in yeast cell extracts. Transcription reactions performed using yeast whole cell extracts reveal that of the three nuclear RNA polymerases only RNA polymerase II function is abrogated when yTAF40 and associated proteins are immunodepleted from solution, indicating that the functionality of the multiprotein complex containing yTAF40 is RNA polymerase II-specific. By these criteria yTAF40 appears to encode a bona fide RNA polymerase II-specific TAF, and thus the protein that it encodes has been termed yTAFII40. | {
"pile_set_name": "PubMed Abstracts"
} |
The KUFP five-visit quit-smoking program.
This article has presented an overview of the Quit-Smoking Program developed and modified at the KUMC Department of Family Practice. Originally, this clinic was designed as a group treatment program, facilitated by a psychologist and physician. Recently, however, the program has been modified for office-based primary care physicians. Because smoking is such a deadly habit, it is hoped that physicians will take a more active role in smoking cessation efforts with their patients who are currently smokers. | {
"pile_set_name": "PubMed Abstracts"
} |
Diabetes: Setting and achieving glycemic goals.
Implementing lifestyle changes can be a substantial challenge for patients with diabetes. This article offers nursing strategies to help patients set and meet glycemic goals and manage their own therapy. | {
"pile_set_name": "PubMed Abstracts"
} |
Current readings: Status of surgical treatment for endocarditis.
Valve endocarditis is associated with high morbidity and mortality and requires a thorough evaluation including early surgical consultation to identify patients who may benefit from surgery. We review 5 recent articles that highlight the current debates related to best treatment strategies for valve endocarditis. Recent publications have focused on neurologic risk assessment, timing of surgery, and prognostic factors associated with native and prosthetic valve endocarditis. The initial patient assessment and management is best performed by a multidisciplinary team. Future investigations should focus on identifying surgical candidates early and the outcomes affected by replacement valve choice in both native and prosthetic valve endocarditis. | {
"pile_set_name": "PubMed Abstracts"
} |
[Analysis of antigen-specific proliferation and IL-2/IL-4 production of spleen cells from mice infected with Mycobacterium bovis BCG].
The present study was performed in order to define the responding populations and profiles of cytokine production in BCG-primed spleen cells restimulated in vitro with antigen. Spleen cells from DBA/2 mice, one of BCG-resistant strains (Bcgr), infected with Mycobacterium bovis BCG vigorously proliferated by restimulation with heat-killed BCG. This response peaked as early as on day 3 after BCG infection, and then decreased to the basal level by 3 weeks. Blocking of IL-2R alpha chain or IL-4 by each antibody partially inhibited it, but anti-IFN-gamma antibody did not, suggesting that both IL-2 and IL-4 were involved in the proliferation of primed spleen cells. CD4+ and gamma delta TCR-bearing T cell were responding populations to BCG, but CD8+ T cell was not, because depletion of CD4+ or gamma delta T cells by the treatment with each antibody and complement inhibited proliferation and IL-2/IL-4 production, but that of CD8+ T cells did not. Further study demonstrated that spleen cells from BCG-resistant DBA/2 mice produced more IL-2/IL-4 than those from BALB/c mice, one of BCG-susceptible strains (Bcgs), in response to BCG. These results suggest that both CD4+ and gamma delta TCR-bearing T cells play an important role in the host defense against M. bovis BCG infection, and that the magnitude of cytokine production is one of the critical factors to define the susceptibility of mice to this pathogen in the late phase of infection. | {
"pile_set_name": "PubMed Abstracts"
} |
Otoacoustic emission criteria for neonatal hearing screening.
Transient evoked otoacoustic emission measures are gaining acceptance as a technique in new-born hearing screening. At present a wide variety of pass-fail screening criteria are used in otoacoustic emission screening programs. In a study of 100 special care neonates and 35 well, full term babies, a number of screening criteria were examined for sensitivity and specificity characteristics when compared to a standard auditory brainstem response protocol. Results indicate that, for normal and special care neonates with a gestational age at test of 38-41 weeks, high sensitivity ( > 80%) could be obtained when a pass-fail criterion involving analysis of emission reproducibility, or emission reproducibility and emission response level, was set. Sensitivity was reduced for special care neonates who fell outside this age range. Specificity was found to be relatively low overall (always < 65%) and may relate to clinical factors in special care neonates not investigated in this study. | {
"pile_set_name": "PubMed Abstracts"
} |
The physical features -size and granularity- of solid materials are strongly affected by trituration in lactose, before turning them into homeopathic solutions.
Trituration is a mechanical process (a form of comminution) for reducing the particle size of a substance. In this manuscript, six different Raw Solid Materials (RSM) which are used in Homeopathy after successive grindings are studied before they are turned into homeopathic solutions. The impact of trituration, with the presence of α‑lactose monohydrate (milk sugar) seems to be quite great and interesting because of the variety of grain size which largely differentiate the properties of the materials. The grain sizes obtained triturations by hand according to C. Herring's suggestion leads, finally, measurement scale dimensions. The obtained results can be useful information for all the pharmacy industries, as well as for preparing any kind of powder. | {
"pile_set_name": "PubMed Abstracts"
} |
Arthrotomography of the temporomandibular joint: new technique and preliminary observations.
A technique for arthrography of the temporomandibular joint using multidirectional tomography is described that greatly simplifies interpretation. In 21 attempts, 20 successful arthrograms were obtained in patients complaining of pain, tenderness, and clicking and locking of the joint who had not responded to conservative therapy. Abnormalities demonstrated were perforation of the meniscus, anterior dislocation and subluxation of the meniscus; and redundancy of the bilaminar zone. In all 11 patients who underwent surgical meniscoplasty, disc abnormalities included thinning, perforation, flaccidity, and bilaminar redundancy. No permanent complications of the arthrographic procedure occurred, but temporary postprocedure discomfort was frequent. This preliminary experience with temporomandibular joint arthrotomography suggests this easily performed procedure offers reliable information about disc function and integrity that may assist in a selection of patients for meniscoplasty and other surgical treatment. | {
"pile_set_name": "PubMed Abstracts"
} |
A novel statistical approach for the evaluation of comet assay data.
The present study forms part of a weight-of-evidence framework including genotoxicological studies in the upper Danube River basin, which aim at elucidating the reasons for the decline in fish catch. The major focus of this paper is the assessment of genotoxicity of sediments from the Danube River basin by use of the comet assay with RTL-W1 cells and with embryos of zebrafish (Danio rerio). A frequently discussed question in this type of approach is how to aggregate and compare the data obtained from genotoxicity testing. There is a need to develop mathematical method combining the information from dose-response curves and level of effectiveness (maximum genotoxic effect). For comparison and ranking of the genotoxic potential of samples from different locations along the Danube River, several methods based on EC50, Lowest Observed Effect Concentration (LOEC), and maximum induction factor were compared with respect to their validity. An evaluation system termed the "3-step analysis" was developed to facilitate consideration of a maximum number of aspects of the raw data. The so-called "concentration-dependent induction factor" (CDI) introduces an index for a straightforward, precise and realistic assessment of the genotoxic potential of any kind of field sample or genotoxic agent. | {
"pile_set_name": "PubMed Abstracts"
} |
Chamazulene reverses osteoarthritic inflammation through regulation of matrix metalloproteinases (MMPs) and NF-kβ pathway in in-vitro and in-vivo models.
This study was conducted to evaluate the protective effects of chamazulene against IL-1β-induced rat primary chondrocytes and complete Freund's adjuvant (CFA)-induced osteoarthritic inflammation in rats. Oxidative stress markers, pro-inflammatory cytokines, and regulatory proteins were measured. Chamazulene significantly reverted (p < 0.05) the levels of lipid peroxidation and enhanced the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) enzymes against IL-1β and CFA-induced oxidative stress. The levels of TNF-α and IL-6 were reduced (p < 0.05) in chamazulene treatment against IL-1β and CFA-induced inflammation. Western blot analysis results on the expressions of MMP-3, MMP-9, p65 NF-kβ, iNOS, and COX-2 showed chamazulene was able to protect the chondrocytes against IL-1β-induced osteoarthritic inflammation. Histopathology of rat hind ankle showed chamazulene significantly protected against CFA-induced osteoarthritic inflammation. Therefore, chamazulene can be recommended as a therapeutic agent for clinical trials against osteoarthritic inflammation. | {
"pile_set_name": "PubMed Abstracts"
} |
Thyroid sonomorphology, thyroid peroxidase antibodies and thyroid function: new epidemiological data in unselected German employees.
Employees of Sanofi-Aventis Deutschland GmbH underwent thyroid screening in 2006 to assess new data about the prevalence of irregular sonomorphological pattern, elevated thyroid peroxidase antibodies (TPO AB) and thyroid function in an unselected adult German population. The examination included 700 unselected employees. Blood samples were analyzed for serum TSH and TPO AB, and ultrasound of the thyroid was performed. In 40.7% of the participants (n = 285) an irregular sonomorphological pattern was detected: goiter in 13.7%, nodules in 35.6%, nodular goiter in 8.6% and a hypoechogenic pattern of the thyroid gland in 20.4%. Serum TSH was increased in 3.9% and decreased in 0.6%. Elevated TPO AB values were observed in 13%. Only 1.4% (n = 10) showed elevated TPO AB combined with a TSH increase. Sonomorphological abnormalities were associated with increased TPO AB in 7.1%. Elevated TPO AB was observed significantly more often in combination with sonomorphological pathology (54.9%) than without (45.1%) (p = 0.003). Sonomorphological disorders are still very common in Germany and our results are comparable with previous screening examinations. Elevated TPO AB correlated significantly with the sonomorphological pattern of nodules and goiter. This may reflect an improved iodine supply or a hypertrophic stage of autoimmune thyroiditis in some cases. | {
"pile_set_name": "PubMed Abstracts"
} |
Evaluation of U.S. Public Health Service programs. Organization and management.
This article provides an overview of evaluation in the Public Health Service (PHS) of the U.S. Department of Health and Human Services. The kinds of evaluation activities are outlined with illustrations of recently completed studies. The overview includes information on resources, policies, and management of evaluations conducted by the various PHS agencies and programs. In particular, the department's new efforts to better disseminate evaluation results through the Internet are described. The article concludes with a discussion of future prospects of how PHS evaluations will play a role in implementation of the Government Performance and Results Act (GPRA). | {
"pile_set_name": "PubMed Abstracts"
} |
Variation of three-dimensional femoral bowing and its relation to physical status and bone mineral density: a study with CT.
Anatomically, the femoral bone shows sagittal and coronal bowing. Pronounced bowing would affect femoral implant surgery accuracy, but factors associated with degrees of three-dimensional (3D) femoral bowing have not yet been identified. This study measured 3D femoral bowing and examined the correlation between 3D femoral bowing and risk factors. 3D femoral bone models of unaffected legs from 125 patients were reconstructed from computed tomography data. A trans-epicondylar axis (TEA)-based coordinate system was introduced into bone models and the reproducibility of this coordinate system was evaluated. The cross-sectional contours of the femoral canal were extracted along the vertical axis of the TEA-based coordinate system, and the angles of 3D femoral bowing were examined. The reproducibility to set the coordinate was very high. Total, sagittal, and coronal femoral bowing were 8.9 ± 1.8°, 8.7 ± 1.8°, and 0.1 ± 1.9°, respectively. There was no significant difference in the degrees of femoral bowing between males and females. Total, coronal, and sagittal femoral bowing correlated with age, height, and femoral length, but only femoral coronal bowing correlated with bone mineral density (BMD) of the femur and lumbar spine. We introduced a highly reproducible method for measuring femoral sagittal and coronal bowing by constructing a femoral 3D coordinate system. Anterior femoral bowing was the dominant direction of bowing in our Japanese cohort, whereas the degree of lateral bowing correlated with age, height, femoral length, and BMD. Our results suggest that lateral femoral bowing could increase with aging and decreasing BMD. | {
"pile_set_name": "PubMed Abstracts"
} |
[Comparable ergometric examinations before and after administration of practolol and bunitrolol (author's transl)].
By means of a standardized, single-level ergometric load of 1.5 watt/kg body weight, the effects of a new beta-adrenergic receptor blocking agent bunitrolol (o-(2-hydroxy-3-tertiary butylamino)-propoxy)-benzonitrile) was compared with the well-known cardiovascular properties of practolol during an intraindividual therapeutic crossover trial after intravenous administration. In the absence of any side-effects, bunitrolol shows a higher degree of beta-adrenergic receptor bocking activity than practolol, with a distinct specificity towards myocardial receptors and very low cardiodepressive properties at rest. Bunitrolol caused a marked reduction (33%) in exercise-induced myocardial work effort, whilst practolol achieved a reduction of only 17% under equal conditions. Thus, the same physical work load is performed after bunitrolol more economically by decreasing both heart rate and blood pressure. The circulatory effects of an intravenous dose of 5 mg bunitrolol seems to be twice as effective as 10 mg practolol. This implies that beta-adrenergic receptor blockade by bunitrolol during work performance is four times as effective as practolol. The observed marked effect of bunitrolol on the diastolic blood pressure is discussed. | {
"pile_set_name": "PubMed Abstracts"
} |
Characteristics of Postpartum Depression in Anand District, Gujarat, India.
Characteristics of postpartum depression (PPD) in Anand District, Gujarat, India. PPD affects 1 in 10 women in the developed world. It has been implicated as an independent factor with adverse effect on child health, and health care-seeking behavior of mothers. We sought to find the prevalence of PPD in our hospital by including mothers who registered and delivered live babies at our hospital. Basic demographic information related to pregnancy was acquired from mothers and Edinburgh Postnatal Depression Scale (EPDS), pre-translated and validated in Gujarati language, was administered. Current study observed prevalence of PPD as 48.5% using cutoff score of 10.5 for classifying depression in Gujarati women. Factors associated with depression after multivariable logistic regression were: age of mother, modified Kuppuswami category (MKC) score, family type, violence from husband, gravida, para and sex of infant. PPD has higher prevalence in our study vis-a-vis Western countries. This may be because of early administration of EPDS. | {
"pile_set_name": "PubMed Abstracts"
} |
Avulsion of the tibial tuberosity with a lateral plateau rim fracture: case report.
An avulsion fracture of tibial tuberosity with an unusual articular involvement was treated with open reduction and internal fixation. Although the mechanism of tibial tuberosity fracture is described as passively flexing the knee while active quadriceps femoris contraction, the weight transmitted from the menisci on the lateral side of the knee joint may cause an epiphyseal disruption. We report a case of tibial tuberosity fracture with lateral plateau rim fracture in a young male adolescent, which has not been published previously in the literature. | {
"pile_set_name": "PubMed Abstracts"
} |
Inter-laboratory validation of the in-vivo flow cytometric micronucleus analysis method (MicroFlow®) in China.
Although inter-laboratory validation efforts of the in-vivo micronucleus (MN) assay based on flow cytometry (FCM) have taken place in the EU and US, none have been organized in China. Therefore, an inter-laboratory study that included eight laboratories in China and one experienced reference laboratory in the US was coordinated to validate the in-vivo FCM MicroFlow(®) method to determine the frequency of micro-nucleated reticulocytes (MN-RETs) in rat blood. Assay reliability and reproducibility were evaluated with four known genotoxicants, and the results obtained with the FCM method were compared with the outcome of the traditional evaluation of bone-marrow micronuclei by use of microscopy. Each of the four chemicals was tested at three sites (two in China and the one US reference laboratory). After three consecutive daily exposures to a genotoxicant, blood and bone-marrow samples were obtained from rats 24h after the third dose. MN-RET frequencies were measured in 20,000 RET in blood by FCM, and micro-nucleated polychromatic erythrocyte (MN-PCE) frequencies were measured in 2,000 PCEs in bone marrow by microscopy. For both methods, each genotoxicant was shown to induce a statistically significant increase in the frequency of MN after treatment with at least one dose. Where more doses than one caused an increase, responses occurred in a dose-dependent manner. Spearman's correlation coefficient (rs) for FCM-based MN-RET vs microscopy-based MN-PCE measurements (eight experiments, 200 paired measurements) was 0.723, indicating a high degree of correspondence between methods and compartments. The rs value for replicate FCM MN-RET measurements performed at the eight collaborative laboratories was 0.940 (n=200), and between the eight FCM laboratories with the reference laboratory was 0.933 (n=200), suggesting that the automated method is very well transferable between laboratories. The FCM micronucleus analysis method is currently used in many countries worldwide, and these data support its use for evaluating the in-vivo genotoxic potential of test chemicals in China. | {
"pile_set_name": "PubMed Abstracts"
} |
Allelic frequencies given the sample's common ancestral type.
A general formula is derived for finding the expected proportion of genes in a random sample of n genes which are of a particular allelic type given the type of the sample's common ancestor. Expressions of these expectations are obtained for the 2-allele, K-allele, infinitely many alleles, and stepwise mutation models. Numerical examples for the stepwise mutation model are also tabulated. | {
"pile_set_name": "PubMed Abstracts"
} |
Endometriosis of the sigmoid colon: a diagnostic problem.
Colonic endometriosis is often difficult to diagnose pre-operatively and most cases are diagnosed postoperatively from histology. The main problems with pre-operative diagnosis are due to the radiographic similarities between endometriosis and malignant lesions and the difficulties in obtaining satisfactory biopsy material. | {
"pile_set_name": "PubMed Abstracts"
} |
Endotoxic activity of cell-free rumen fluid from cattle fed hay or grain.
The cell-free rumen fluid from cattle fed hay or grain exhibited the following biological characteristics which strongly suggest the presence of endotoxin or a toxic principle similar to endotoxin of gram-negative bacteria: proved lethal to mice when injected with actinomycin D; proved extremely lethal to chick embryo; induced biphasic pyogenic response in rabbits; enhanced susceptibility to bacterial infection in mice; evoked positive epinephrine skin reaction in rabbits and phenol-water or aqueous ether proved lethal to mice and chick embryos. A quantitative difference in concentrations of endotoxin was observed on LD50 in mice and chick embryos and response to the epinephrine skin test in rabbits. Cell-free rumen fluid of grain-fed cattle contained at least twice as much endotoxin as that of hay-fed cattle. Endotoxin in cell-free rumen fluid and in higher concentration in cattle fed grain than in those fed hay support the hypothesis that rumen bacterial endotoxins may participate in the pathogenesis of diseases associated with high grain feeding such as lactic acidosis and the sudden-death syndrome. | {
"pile_set_name": "PubMed Abstracts"
} |
Clinical clearance of cervical spinal injuries by emergency nurses.
To determine the interrater reliability between emergency nurses and emergency physicians on defined criteria for clinically clearing the cervical spine in blunt trauma patients. Blunt trauma patients, 12 years or older, arriving with cervical spinal precautions were prospectively enrolled as a convenience sample. Each member of the emergency physician-nurse pair completed a questionnaire with regard to five criteria for clinically clearing the cervical spine for each patient. Interrater reliability was determined by calculating the kappa statistics for the individual and combined criteria. Physicians and nurses agreed on the presence or absence of the combined criteria in 175 of 211 patients (82.9%; kappa, 0.65). Agreements on individual criteria were as follows: 1) intoxication--203 patients (96.2%; kappa, 0.82); 2) altered consciousness--197 patients (93.4%; kappa, 0.60); 3) neck pain--185 patients (87.7%; kappa, 0.75); 4) distracting injury--160 patients (75.8%; kappa, 0.36); and 5) neurologic deficit--198 patients (93.8%; kappa, 0.45). If disagreements in which the physician would clinically clear the patient but the nurse would not were considered as agreements, then overall agreement would be 198 of 211 patients (93.8%; kappa, 0.88). On the assumption that nurses would assess patients prior to physicians, they would have cleared 35% of the patients before the physicians. However, they would have ordered 12% more radiographs and unsafely clinically cleared 5% of the patients. The interrater reliability for the combined cervical spinal injury criteria between emergency nurses and physicians was good to excellent. However, with the training given in this study, nurses would order more radiographs than physicians and would unsafely clinically clear cervical spines in some patients. | {
"pile_set_name": "PubMed Abstracts"
} |
Membrane-bound structure and energetics of alpha-synuclein.
Aggregation and fibrillation of alpha-synuclein bound to membranes are believed to be involved in Parkinson's and other neurodegenerative diseases. On SDS micelles, the N-terminus of alpha-synuclein forms two curved helices linked by a short loop. However, its structure on lipid bilayers has not been experimentally resolved. Using MD simulations with an implicit membrane model we show here that, on a planar mixed membrane, the truncated alpha-synuclein (residues 1-95) forms a bent helix. Bending of the helix is not due to the protein sequence or membrane binding, but to collective motions of the long helix. The backbone of the helix is approximately 2.5 A above the membrane surface, with some residues partially inserted in the membrane core. The helix periodicity is 11/3 (11 residues complete three full turns) as opposed to 18/5 periodicity of an ideal alpha-helix, with hydrophobic residues towards the membrane, negatively charged residues towards the solvent and lysines on the polar/nonpolar interface. A series of threonines, which are characteristic for alpha-synuclein and perhaps a phosphorylation site, is also located at the hydrophobic/hydrophilic interface with their side chain often hydrogen bonded to the main-chain atom. The calculations show that the energy penalty for change in periodicity from the 18/5 to 11/3 on the anionic membrane is overcome by favorable solvation energy. The binding of truncated alpha-synuclein to membranes is weak. It prefers anionic membranes but it also binds marginally to a neutral membrane, via its C-terminus. Dimerization of helical monomers on the mixed membrane is energetically favorable. However, it slightly interferes with membrane binding. This might promote lateral diffusion of the protein on the membrane surface and facilitate assembly of oligomers that precede fibrillation. | {
"pile_set_name": "PubMed Abstracts"
} |
Characterization of the gluconate utilization system of Vibrio cholerae with special reference to virulence modulation.
Orthologs search identified that the Vibrio cholerae gluconate (Gnt) utilization system minimally consisted of the Entner-Doudoroff (ED) pathway (edd and eda) and three other genes, namely gntU, gntK and gntR This system appeared unique by genomic organization of component genes into two operons transcribed in opposite directions. In silico analysis indicated GntU as an inner-membrane protein functioning for transport and GntK as a kinase with cytosolic localization that generates Gnt6P, which is then metabolized through the ED pathway. Enzyme 6-phosphogluconate dehydratase encoded by edd converts Gnt6P to 2-keto-3-deoxy-6-phosphogluconate (KDPG), which is metabolized by the action of KDPG-aldolase encoded by eda Transcriptional upregulation of the Gnt utilization genes in the gntR mutant matched well to a predicted repressor role of GntR. GntR displayed DNA binding to a region in the promoters of two bi-directionally transcribed operons. Growth defect of mutants in Gnt-supplemented media confirmed obligate involvement of these genes in Gnt utilization and such defect was restored upon complementation. Defective Gnt utilization resulted in attenuation of colonization potential and reduction of cholera toxin secretion in V. cholerae The ED pathway mutants showed the highest level of virulence attenuation. Overall, this study established a minimal requirement of the V. cholerae Gnt utilization system, which played a critical role in pathogenesis. | {
"pile_set_name": "PubMed Abstracts"
} |
Photorefraction of the living eye: a model for linear knife edge photoscreening.
A computer model is developed to explain colored artifacts in pupil images of the living human eye formed by the linear knife edge photoscreener. An approximate expression for the RGB irradiances recorded by the photoscreener is derived by using the statistical properties of the line spread functions that characterize the source, the aberrations of the eye, and the reflecting properties of the retina. The pupil image for each color is shown to be very much a map of the deviations perpendicular to the knife edge imposed on rays by an equivalent aberration plate, and the overall response of the photoscreener to specific aberrations of the eye is cataloged for errors of accommodation and chromatic aberration, spherical aberration, astigmatism, and tear bands. | {
"pile_set_name": "PubMed Abstracts"
} |
A downloadable meshed human canine tooth model with PDL and bone for finite element simulations.
The aim of this study is to relieve scientists from the complex and time-consuming task of model generation by providing a model of a canine tooth and its periradicular tissues for Finite Element Method (FEM) simulations. This was achieved with diverse commercial software, based on a micro-computed tomography of the specimen. The Finite Element (FE) Model consists of enamel, dentin, nerve (innervation), periodontal ligament (PDL), and the surrounding cortical bone with trabecular structure. The area and volume meshes are of a very high quality in order to represent the model in a detailed form. Material properties are to be set individually by every user. The tooth model is provided for Abaqus, Ansys, HyperMesh, Nastran and as STL files, in an ASCII format for free download. This can help reduce the cost and effort of generating a tooth model for some research institutions, and may encourage other research groups to provide their high quality models for other researchers. By providing FE models, research results, especially FEM simulations, could be easily verified by others. | {
"pile_set_name": "PubMed Abstracts"
} |
What Have We Learned From the Osaka Study?
To summarize the results of a cross-sectional epidemiologic study of Japanese office workers who use visual display terminals (VDTs). Workers in a pharmaceutical company head office were recruited. Participants were requested to complete a questionnaire on symptoms of dry eye disease (DED) and evaluations using the Work Limitations Questionnaire, International Physical Activity Questionnaire, Subjective Happiness Scale, and Pittsburgh Sleep Quality Index. Evaluations of ocular status, including conjunctival and corneal vital staining with lissamine green and fluorescein, measurement of tear film breakup time, and the Schirmer test, were performed. The Japanese 2007 diagnostic criteria for DED were implemented. The prevalence of DED among VDT workers was high. Definite DED was present in 11.6% (18.7% women and 8.0% men) of participants, and probable DED was present in 54.0% (57.8% women and 52.1% men). Total loss of workplace productivity was significantly greater in the definite DED group (4.82%) than in the probable DED group (4.06%) and the non-DED group (3.56%, P < 0.014, trend test). Participants with definite DED had significantly lower physical activity scores than those without DED (P = 0.025). Subjective happiness correlated with the dry eye symptom score (r = -0.188; P < 0.001). Participants with DED reported poor sleep quality at a higher rate compared with those without DED (45% vs. 34%), with a significant difference in the global score (P = 0.002). Dry eye is prevalent among VDT workers. Its impacts on work productivity, physical activity, and sleep quality warrant further study. | {
"pile_set_name": "PubMed Abstracts"
} |
Treatment of advanced measurable gastric carcinoma with 5-fluorouracil, adriamycin, and BCNU.
61 patients with advanced measurable gastric carcinoma were treated with 5-fluorouracil, adriamycin and BCNU (FAB). Of 50 evaluable patients, 48% achieved a response (3 complete and 21 partial responses), and 24% experienced disease stabilization. The median duration of response was 9 months, and median survival of all patients was 7 months. Responding patients survived significantly longer than nonresponders (p less than 0.001). Toxicity was generally mild to moderate, rarely requiring dose reduction or prolongation of treatment intervals. These results indicate that FAB regimen is an active combination in metastatic or locally advanced gastric cancer, and can be used as first-line treatment in patients with this disease. | {
"pile_set_name": "PubMed Abstracts"
} |
Direct and indirect "cortico"-rubral and rubro-cerebellar cortical projections in the pigeon.
In birds the red nucleus is the most rostral cell group in the brain having projections to all levels of the spinal cord (Cabot et al., Prog. Brain Res., 57:79-108, 1982), but its sources of afferents are incompletely known. In order to determine these, a series retrograde and anterograde tracing experiments was carried out, largely with cholera toxin B-chain conjugated to horseradish peroxidase. The results show that a sparse and diffuse projection to the red nucleus arises from deep regions of the hyperstriatum accessorium (HA) of the anterior Wulst, and that a much more dense projection arises from the caudal part of the nucleus principalis precommissuralis and the medial part of the medial spiriform nucleus (SpMm). These last two sources were themselves shown to receive a substantial projection from HA of the anterior Wulst. The red nucleus was also shown to project upon the cerebellar cortex of lobule VI, and SpM upon the cerebellar cortex of lobules VI through IX (Karten and Finger, Brain Res., 102:335-338, 1976; Clarke, J. Comp. Neurol., 174:535-552, 1977). Double retrograde labelling experiments with fluorescein and rhodamine labelled latex microspheres injected into the cerebellar cortex and spinal cord showed that the rubrocerebellar cortical neurons are a different population from, although intermixed with, the rubrospinal neurons. | {
"pile_set_name": "PubMed Abstracts"
} |
Molecular properties and extracellular processing of the lipase of Staphylococcus warneri M.
Staphylococcus warneri M exhibited extracellular lipase activity. By zymogram analysis of extracellular proteins, multiple bands were detected and the profiles changed depending on the bacterial growth phase. N-terminal amino acid sequences of three bands (N1-N3) were determined. From the genome library of S. warneri M whole DNA, the gene-directing lipase activity (named gehC(WM)) was cloned and characterized. The gehC(WM )gene encoded a protein (GehC(WM)), whose calculated molecular mass was 83.4 kDa, and the sequence was similar to the other staphylococcal lipases. Though two lipases have been known from S. warneri 863, GehC(WM) differs from both of them, indicating that this enzyme is the third extracellular lipase of the S. warneri strain. The N-terminal sequences of the N1-N3 polypeptides completely coincided with the deduced amino acid sequences in GehC(WM). GehC(WM) was predicted to be a prepro-protein. In vitro processing and protein sequencing suggested that pro-GehC(WM) is possibly processed by extracellular glutamyl endopeptidase, PROM. Inductively coupled plasma-atomic emission spectrometer analysis showed that purified his-tagged mature GehC(WM) possessed zinc ion. A gehC(WM) knockout mutant was constructed by insertion of an erythromycin resistance gene into the gehC(WM). Zymogram and immunoblot analyses of the gehC(WM )mutant indicated that GehC(WM) was a major extracellular lipase of S. warneri M. | {
"pile_set_name": "PubMed Abstracts"
} |
Natural population die-offs: causes and consequences for terrestrial mammals.
Extreme changes in the environment can generate high mortalities in wildlife populations. When these mortalities are attributable to extreme natural events, they are referred to as natural population die-offs. Despite growing reports of such die-offs, a consensus on how to define them has not emerged. Furthermore, although anthropogenically caused extreme events are predicted to occur at a higher frequency and intensity compared with natural events, an integrative synthesis assessing their significance for wildlife population viability is lacking. These issues hamper the ability to identify populations most at risk. Here, we propose a functional definition of natural population die-offs, an assessment of extrinsic and intrinsic processes shaping these die-offs, and a framework for assessing the vulnerability of terrestrial mammals to natural and anthropogenically caused extreme events. | {
"pile_set_name": "PubMed Abstracts"
} |
Asthma: a simple concept but in reality a complex disease.
Asthma is a disorder of the conducting airways that contract too easily and too much to cause variable airflow obstruction with symptoms of wheeze, cough, chest tightness and shortness of breath. Based on this knowledge, initial treatments were directed to dilating the contracted airways with anticholinergic and adrenergic drugs. The recognition that allergic-type inflammation underlay the hyperresponsive airways in asthma led to the introduction of anti-inflammatory drugs such as sodium cromoglicate and corticosteroids. Over the 2 decades that followed, these drugs have been progressively improved by increasing their therapeutic index and duration of action. A review of the recent literature indicates that since the 1980s, the explosive increase in knowledge of the cell and mediator mechanisms of asthma has only led to modest improvements in therapy including the introduction of leukotriene modifiers and a blocking monoclonal antibody against IgE. Indeed, biologics targeting allergic cytokines and effector cells have on the whole proven disappointing despite initial promise being shown in animal models. Part of the difficulty lies in the oversimplified concept that asthma is only driven by allergic processes when in reality there are many environmental causes and triggers and the view that it is a homogeneous disorder only varying in severity. The more recent views that asthma is a complex disorder made up of different subtypes with differing causes, treatment responses and natural histories creates a new opportunity for stratified medicine in which therapies acting upstream selectively target specific disease subtypes identified by specific diagnostic biomarkers. | {
"pile_set_name": "PubMed Abstracts"
} |
Increased vascular endothelial growth factor production in fibroblasts isolated from strictures in patients with Crohn's disease.
Vascular endothelial growth factor (VEGF) is a potent angiogenic factor that is implicated in early wound healing and fibrosis. Fibroblasts may initiate stricture formation in Crohn's disease through overexpression of VEGF. The aim of this study was to examine VEGF expression and regulation in fibroblasts isolated from patients with Crohn's disease. Fibroblasts were isolated by a primary explant technique from serosal biopsies of non-strictured and strictured segments of bowel from eight patients undergoing resection for Crohn's disease, and normal colon from six patients undergoing resection for benign and malignant colorectal disease. Fibroblasts were cultured with transforming growth factor (TGF) beta and corticosteroids. After 24 h the culture supernatant was collected for VEGF assay by enzyme-linked immunosorbent assay. VEGF production was significantly higher in fibroblasts isolated from strictures (mean(s.e.m.) 1980(260) pg/ml) than from non-strictured segments (1116(165) pg/ml) in patients with Crohn's disease or control fibroblasts (898(93) pg/ml). TGF-beta increased VEGF production in normal and non-strictured Crohn's fibroblasts. Corticosteroids suppressed unstimulated VEGF production in all groups. Enhanced serosal fibroblast VEGF production might play a role in initiating stricture formation in Crohn's disease. VEGF production in serosal fibroblasts is sensitive to stimulation with TGF-beta. Corticosteroids may reduce stricturing through suppression of VEGF. | {
"pile_set_name": "PubMed Abstracts"
} |
[Clinical observation of qiyao xiaoke capsule in intervening 76 patients with type 2 pre-diabetes].
To observe the clinical effects of Qiyao Xiaoke Capsule (QXC) on patients with type 2 pre-diabetes. Totally 116 pre-diabetes patients were randomly assigned to the Chinese medicine group (CM, 76 cases) and the blank control group (BC, 40 cases) in the ratio of 2: 1. All patients received proper diet control, health education, and exercises, and so on. Besides, patients in the CM group took QXC (0.4 g/pill), 6 pills each time, three times a day. But patients in the BC group were intervened by life style alone. The fasting blood glucose (FBG), postprandial blood glucose (PBG), insulin (FINS, 2h INS), glycosylated hemoglobin (HbAlc), blood lipids (TG, TC, HDL-C, and LDL-C), and efficacy of CM symptoms were observed in the two groups before and after intervention. The sequelae were observed at the end of the treatment and at follow-ups. After treatment FBG, PBG, and HbA1c decreased in all patients of the two groups (P<0.05, P<0.01), with 2 h PBG decreased more significantly. But there was no statistical difference between the two groups (P>0.05). The two methods could improve the secretion of FINS. Especially 2 h INS decreased more significantly in the CM group, showing statistical difference when compared with the BC group (P<0.05). The two methods could improve the metabolism of blood lipids. CM could significantly lower TG and elevate HDL-C, showing statistical difference when compared with before treatment (P<0.05). After treatment the CM symptoms were obviously improved, showing statistical difference when compared with the BC group (P<0.05). The normalization rate was better in the CM group than in the BC group at the end of the treatment and at follow-ups (P<0.05). QXC combined life style intervention could improve fasting and postprandial insulin secretion of type 2 pre-diabetes patients, regulate glycolipid metabolism, correct the insulin resistance state, and improve the symptoms of qi-yin insufficiency. It could postpone or hinder the occurrence and development of type 2 diabetes. It was more effective and durable than changing the life style alone. | {
"pile_set_name": "PubMed Abstracts"
} |
Diagnostic and psychopharmacological treatment characteristics of 536 inpatients with posttraumatic stress disorder.
Descriptive information (demographic variables, DSM-III diagnoses, and medications prescribed) was obtained from the discharge summaries of 536 male veteran inpatients who received a diagnosis of posttraumatic stress disorder (PTSD) over a 4-year period. Data comparisons were also made between two types of inpatient programs (specialized PTSD vs. psychiatric ward) to control for program selection biases. Consistent with previous studies, alcohol and substance abuse and/or depression diagnoses occurred concurrently with PTSD. Diagnoses of schizophrenia were more prevalent in the psychiatric wards. Nearly one third of the total sample received an axis II diagnosis, with borderline features most common. Half of all patients received psychopharmacological treatment in addition to psychotherapy. Antidepressants, neuroleptics, and beta-blockers were prescribed most frequently. Directions for future inpatient PTSD research are offered based on the findings. | {
"pile_set_name": "PubMed Abstracts"
} |
Improving lower limb salvage following fractures with vascular injury: a systematic review and new management algorithm.
Lower limb fractures with vascular injuries are associated with a high rate of secondary amputation. Reducing ischaemic time is vital for limb salvage. However, the optimal sequence of surgical management remains unclear. We aimed to review the literature to establish an evidence-based management algorithm. All identifiable English language or translated literature related to the surgical sequence of lower limb fractures with vascular injuries was reviewed. A total of 101 cases described in 10 publications (median age: 31; range: 2.5-76) were suitable for analysis. The mean MESS was 4.2. The limb-salvage rate with an ischaemic time of less than 6h was 87%, falling to 61% when ischaemic time exceeded 6h. A preoperative angiography caused a significant delay. The rate of re-vascularisation within 6h improved from 46% (33 of 71) to 90% (27 of 30) with the use of a shunt (p=0.04), with a mean ischaemic time of 3.8h (+/-1.7h, 1 standard deviation (SD)) versus 7.6h (+/-3.8h, 1SD) in those re-vascularised using grafts (p<0.001). The amputation rate of 27% was reduced to 13% by using shunts. Early recognition of vascular injury is vital. Formal angiograms are unnecessary and cause crucial delays. A vascular shunt can significantly reduce ischaemic time, enabling unhurried assessment of the feasibility of limb salvage, debridement of demonstrably non-viable tissue and safe skeletal fixation prior to definitive vascular and soft-tissue repair. | {
"pile_set_name": "PubMed Abstracts"
} |
A teratogenicity study of Phloxine B in ICR mice.
Pregnant Jcl:ICR mice were given Phloxine B in the diet at concentrations of 0, 1, 3 and 5% from the morning of day 6 through day 16 of gestation. The mice were killed on day 18 and foetuses were examined for external, visceral and skeletal anomalies. A significant decrease in body-weight gain was observed in all of the treated groups. Among the dams in the high-dose group, two maternal deaths, one abortion and a significant increase in liver weight were observed. A dose-related incidence of splitting of the cervical vertebral arches (nos 3-6) was noted in all of the treated groups, but this anomaly was not found in the controls. The total incidence of skeletal anomalies was also dose related and was significantly increased at the 3 and 5% dose levels. It was concluded that Phloxine B was teratogenic in mice at dietary levels of 3 and 5%, levels which resulted in maternal toxicity, and that a finding suggesting a teratogenic effect (split cervical arches) was also noted at the 1% dose level. | {
"pile_set_name": "PubMed Abstracts"
} |
Therapeutic approaches to raising plasma HDL-cholesterol levels.
Epidemiologic data from the Framingham and Prospective Cardiovascular Munster studies, demonstrating an inverse correlation between the plasma concentration of HDLs and the incidence of cardiovascular disease, have driven research to explore precisely how HDLs confer this cardioprotective effect. HDLs are anti-inflammatory, antithrombogenic and have vasoactive effects, as well as being efficient cholesterol acceptors enabling the removal of cholesterol from peripheral tissues, all functions that are likely to protect the vasculature. The first part of this article will review the clinical evidence in support of the pleiotropic effects of HDLs, along with laboratory-based investigations of the molecular mechanisms of action. As the evidence of clinical benefits of raising plasma HDL concentration has increased, so has the number of strategies currently being considered to achieve this goal. The second part of this article will review three current strategies: infusion of HDL-like products, comparing physicopharmacologic characteristics of the two commercial products currently under trial; the use of fibrates to raise plasma HDLs (although fibrates primarily reduce triglyceride levels, certain derivatives are able to induce significant increases in plasma HDLs); and the use of drugs that inhibit cholesterol ester transfer protein (these drugs increase plasma HDL concentration either alone or as an adjunct therapy with statins). The clinical efficacy and mechanism of action of fibrates and inhibitors of cholesterol ester transfer protein will be reviewed. | {
"pile_set_name": "PubMed Abstracts"
} |
Autophagic tumor stroma: mechanisms and roles in tumor growth and progression.
Macroautophagy (hereafter autophagy) is a cellular homeostatic mechanism that involves protein and organelle degradation, and has a number of connections to human physiology and diseases. Autophagy in tumor parenchyma acts as either a tumor-promoting role or a tumor-inhibiting role depending on the types and stages of tumors. In recent years, attention to autophagy in tumor stroma that is referred as "autophagic tumor stroma" has created a new paradigm to understand the role of autophagy in cancer. Here we propose that the autophagic tumor stroma is a phenomenon of adaptation at a certain stage of tumor development, and has a prominent role in tumor growth, progression and spread of tumors. This idea is supported by recent studies: (i) Autophagic tumor stroma is activated by hypoxia and cancer cells induced oxidative stress, when tumors grow to a certain stage; (ii) Autophagic tumor stroma aids in providing essential nutrients to malignant cells, remodeling the tumor microenvironment, increasing DNA damage, genetic instability and stemness in cancer cells, and decreasing the apoptotic sensitivity of cancer cells. The autophagic tumor stroma is therefore a significant determinant in tumor growth and progression and implicates an important target for cancer therapies. | {
"pile_set_name": "PubMed Abstracts"
} |
A study of person-environment fit among the chronically ill.
The ecological perspective is gaining acceptance in social work thinking. This approach focuses on the interaction of people and situations. An important ecological concept is person-environment fit; that is, the congruency between people needs, capacities, and aspirations and the resources demands and opportunities in their environment. The study described in this article explored the empirical manifestations of this concept among a group of chronically ill persons. The results suggested that there are several categories of person-environment fit that are important to people. The concept also appears to have potential usefulness for research on social work practice. | {
"pile_set_name": "PubMed Abstracts"
} |
[Potential for neural regeneration in the adult mammalian retina].
It has long been believed that the retina of mature mammals is incapable of regeneration. However, here we show that Müller glia of adult mammals could be progenitor cells, and generate new retinal neurons. N-methyl-D-aspartate(NMDA) was injected into the vitreous chamber of adult rat (postnatal 6-7 weeks) eyes to induce neurotoxic injury. We injected bromo-deoxyuridine (BrdU) into the vitreous chamber and intraperitoneal space, and performed immunohistochemistry staining for BrdU and cell specific markers. To test whether exogenous growth factors stimulate proliferating cells, we injected retinoic acid into the vitreous chamber after NMDA treatment. We next misex-pressed basic helix-loop-helix (bHLH) and homeobox genes in NMDA-treated retinas using a retroviral expression system. Müller glia of adult mammalian retinas proliferated in response to acute damage two days after NMDA treatment. These cells acquired a progenitor-like phenotype, and some of them migrated to the outer nuclear layer (ONL). A few of these cells expressed bipolar specific or rod photoreceptor specific markers. Retinoic acid treatment increased bipolar cell genesis. Misexpression of Math3 or NeuroD along with Pax6 promoted differentiation to amacrine cells. Co-expression of Crx and NeuroD promoted rod genesis. These findings demonstrated that retinal neurons regenerated even in adult mammalian retinas after toxic injury. We could partially control the fate of the regenerated neurons with extrinsic factors or intrinsic genes. | {
"pile_set_name": "PubMed Abstracts"
} |
DNA-binding properties of phosphorylated and dephosphorylated D2-T antigen, a simian-virus-40 T-antigen-related protein.
I have examined the role of phosphorylation of D2-T antigen in its DNA-binding properties and ATPase activity. Treatment of partially purified D2-T antigen with alkaline phosphatase resulted in removal of maximally 90% of the phosphate label associated with the radio-labeled protein. The specific and nonspecific DNA-binding properties of partially dephosphorylated D2-T antigen were identical to those of the untreated control. In contrast, acid phosphatase was able to dephosphorylate D2-T antigen quantitatively. The general affinity for DNA of the completely dephosphorylated protein was unchanged or eventually slightly increased. However, its specific affinity for a restriction fragment containing the canonical T-antigen-binding sites was drastically reduced as shown by competition with unlabeled salmon sperm DNA. The results imply that nonspecific DNA binding of D2-T antigen is unaffected by phosphorylation whereas a specific phosphorylation site seems to be involved in the formation and/or stabilization of the specific protein-DNA complex. On the other hand, the ATPase activity of D2-T antigen seems to be unaffected by the degree of phosphorylation. | {
"pile_set_name": "PubMed Abstracts"
} |
An assay for colony-stimulating factor (CSF) production by single T lymphocytes: estimation of the frequency of cells producing granulocyte-macrophage CSF and multi-lineage CSF within a T lymphocyte clone.
The frequency of cells producing hemopoietic colony-stimulating factors (CSF) in a murine T lymphocyte clone has been determined by using a simple microassay that does not require clonal expansion or the addition of accessory cells. When stimulated with concanavalin A (Con A), the clone LB3 produced both granulocyte-macrophage CSF (GM-CSF) and multi-lineage CSF (Multi-CSF), which could be detected by using the cell line FDC-P1, whose proliferation is dependent on the presence of either of these factors. Limiting dilution analysis of Con A-stimulated LB3 cells indicated a requirement for cell-cell contact for optimal production of CSF, which could be bypassed by preincubation of the cells at high density with Con A for 4 hr before dilution in the assay. Limiting dilution estimates of the frequency of CSF-producing cells among Con A-pretreated LB3 cells ranged from 20 to 50%. Direct measurement of CSF production by single Con A-pretreated cells isolated by micromanipulation revealed that 10 to 20% could secrete detectable CSF. However, when isolated Con A-pretreated two-cell and three-cell aggregates were assayed, 50 to 99% were positive, indicating that 30 to 80% of the cells in the aggregates secreted CSF. Assay of the supernatants from single cells and two-cell aggregates on both FDC-P1 cells and another cell line, 32D c13, which responds only to Multi-CSF, demonstrated that many cells produced GM-CSF only, and others varied in the relative quantities of GM-CSF and Multi-CSF produced. | {
"pile_set_name": "PubMed Abstracts"
} |
The effects of halothane on sympathetic ganglionic transmission.
The effects of halothane on ganglionic transmission were studied in the stellate ganglion of the guinea pig using intracellular recordings in vitro. Depression of synaptic transmission is one of the actions common to many general anesthetics. The aim of this study was to investigate which of the processes involved in synaptic transmission are affected by halothane in concentrations comparable to those used during surgical anesthesia. The neurons of the stellate ganglion were depolarized using preganglionic nerve stimulation, postganglionic nerve stimulation, and intracellular stimulation before ad after introduction of halothane (vaporizer settings of 0.75% and 1.5% produced bath concentrations of 8 and 18 mg/dl, respectively). Halothane at both concentrations depressed sympathetic ganglionic transmission which was induced by stimulation of preganglionic nerves. Axonal transmission and the excitability of the postganglionic neurons to direct intracellular stimulation was far less sensitive to halothane than synaptic transmission. The depression of ganglionic transmission seen in the present study was most likely due to a decrease in transmitter release although alterations in postsynaptic receptor properties could have been involved as well. The decrease in sympathetic activity resulting from depression of ganglionic transmission probably contributes to the arterial hypotension seen during halothane anesthesia, along with direct myocardial depression, inhibition of catecholamine release from the adrenal medulla, direct action on vascular smooth muscle, and central sympathetic depression. | {
"pile_set_name": "PubMed Abstracts"
} |
High CXCR4 and low VLA-4 expression predicts poor survival in adults with acute lymphoblastic leukemia.
Data regarding the prognostic significance of CXCR4 and VLA-4 in ALL are limited. Especially, VLA-4 has not been evaluated at the time of diagnosis in both adult and childhood ALL patients. We prospectively analyzed the expression of VLA-4 and CXCR4 in 54 patients (VLA-4 in 29 adults and 25 children and CXCR4 in 22 adults and 24 children) newly diagnosed with ALL by flow cytometry. Expression levels of VLA-4 and CXCR4 were not different between adults and children with ALL. High CXCR4 and low VLA-4 expression each correlated with worse prognosis in adults; patients with high CXCR4 expression had shorter disease-free survival (p=0.01) and overall survival (p=0.04) and patients with low VLA-4 expression had shorter disease-free survival (p=0.02). Expression levels of CXCR4 and VLA-4 did not predict patient prognosis in children. Analysis of CXCR4 and VLA-4 expression at diagnosis in adults with ALL can provide useful information on patient prognosis. | {
"pile_set_name": "PubMed Abstracts"
} |
[Experiences and results with the Endologix-bifurcated endovascular graft].
Since the first endograft implantation for treatment of abdominal aneurysms the development of modern stentgrafts has progressed in many ways. In 1999 we started implantations using the PowerLink aortic stent graft by Endologix-company. The PowerLink aortic stent graft is a bifurcated, self-expanding, sutureless endovascular graft, covered with PTFE. This aortic stent graft facilitates the complete covering of the infrarenal aorta and the iliac arteries. The anatomical localization at the natural aortic bifurcation provides anti-gravitational supporting force, preventing distal migration of the device. From 1999 to 2005 we implanted 297 PowerLink aortic stent grafts. Our follow-up examinations showed a low rate of renal infarctions (3.0 %), a limb occlusion rate of 2.7 % and a total endoleakage rate of 16.8 %. Our conclusion is, that this is a very safe device for repair of abdominal aneurysms, associated with a low-risk of complications. | {
"pile_set_name": "PubMed Abstracts"
} |
[Projective identification, mimesis and the analytical situation. Preliminary observations].
In an attempt to underline the need to refer to an imaginary setting, in which the analytical relationship is acted out, the Authors have considered the possible relations between the concept of projective identification, as defined by Klein and further developed by Bion, and the idea of "Mimesis", which is inevitably involved in every story, and which confronts the imaginary at the very moment in which it is produced. The "fusion" between subject and object, which may occur in a more or less partial manner, is defined as a phenomenal demonstration of the participation of the two poles of the relationship in a "super-individual" experience which embraces them both. The mythical image of the hunter. Anyone is, in our opinion, a paradigmatic clement in this from of "meeting" which takes place within an impersonal and illusionary dimension. | {
"pile_set_name": "PubMed Abstracts"
} |
Diagnosis and Treatment of Non-Muscle Invasive Bladder Cancer: AUA/SUO Guideline.
Although associated with an overall favorable survival rate, the heterogeneity of non-muscle invasive bladder cancer (NMIBC) affects patients' rates of recurrence and progression. Risk stratification should influence evaluation, treatment and surveillance. This guideline attempts to provide a clinical framework for the management of NMIBC. A systematic review utilized research from the Agency for Healthcare Research and Quality (AHRQ) and additional supplementation by the authors and consultant methodologists. Evidence-based statements were based on body of evidence strength Grade A, B, or C and were designated as Strong, Moderate, and Conditional Recommendations with additional statements presented in the form of Clinical Principles or Expert Opinions.(1) RESULTS: A risk-stratified approach categorizes patients into broad groups of low-, intermediate-, and high-risk. Importantly, the evaluation and treatment algorithm takes into account tumor characteristics and uniquely considers a patient's response to therapy. The 38 statements vary in level of evidence, but none include Grade A evidence, and many were Grade C. The intensity and scope of care for NMIBC should focus on patient, disease, and treatment response characteristics. This guideline attempts to improve a clinician's ability to evaluate and treat each patient, but higher quality evidence in future trials will be essential to improve level of care for these patients. | {
"pile_set_name": "PubMed Abstracts"
} |
Suspected iatrogenic paradoxical embolization in a cat.
A cat was evaluated for a 5-year history of progressive, episodic, exercise-induced cyanosis and panting. Diagnostic testing demonstrated tetralogy of Fallot with predominant right-to-left shunting and right-sided heart failure. Following diagnostic catheterization, the cat developed clinical signs consistent with systemic arterial thromboembolization and was euthanized. Necropsy findings included multiple thrombi within the right atrium and ventricle, and thromboemboli within the terminal aorta and right common carotid artery, a condition most consistent with iatrogenic paradoxical embolization secondary to diagnostic catheterization. Paradoxical embolization and thromboembolic complications of diagnostic catheterization are discussed. | {
"pile_set_name": "PubMed Abstracts"
} |
A randomized comparison of combined ticlopidine and aspirin therapy versus aspirin therapy alone after successful intravascular ultrasound-guided stent implantation.
Previous studies have shown that it is feasible to withhold anticoagulation after a successful intracoronary stent procedure with a low incidence of stent thrombosis. The importance of specific antiplatelet agents when stenting is performed without anticoagulation is unknown. After successful intravascular ultrasound-guided stenting, 226 patients were randomly assigned to receive either aspirin therapy alone (n = 103) or a combination of ticlopidine and short-term aspirin therapy (n = 123). Primary angiographic and clinical end points were stent thrombosis, death, myocardial infarction, the need for postprocedure coronary artery bypass surgery or repeated angioplasty, and significant medication side effects requiring termination of the medication within the first month of a successful procedure. At 1 month, the rate of stent thrombosis was 2.9% in the aspirin only group and 0.8% in the ticlopidine-aspirin group (P = .2). Cumulative major clinical events after successful stenting occurred in 3.9% of the patients in the aspirin group and in 0.8% in the ticlopidine-aspirin group (P = .1). There were no medication side effects in the aspirin group; in the combined ticlopidine-aspirin group, medication side effects occurred in 3 patients (P = .2). At 1 month, there was no difference in the incidence of stent thrombosis or other clinical end points between the two poststent antiplatelet regimens. However, the relatively small size of the study and the low incidence of thrombosis events may have contributed to the failure to detect differences in angiographic and clinical end points between the two groups. | {
"pile_set_name": "PubMed Abstracts"
} |
Consumer health information and local health resources: MedlinePlus and My Health Minnesota --> Go Local Outreach Efforts.
The University of Minnesota Health Sciences Libraries and an NLM Public Health Informationist Fellow are designing, implementing and evaluating outreach and training related to the My Health Minnesota --> Go Local project. The goal is to enhance the skills of public health and community based organizations in assisting community members with health information needs. Ultimately, this project seeks to improve health literacy among Minnesota citizens. | {
"pile_set_name": "PubMed Abstracts"
} |
[Pulmonary involvement due to HHV-8 virus during the course of HIV infection].
HHV-8 is a herpes virus discovered in 1994 in Kaposi sarcoma cells. Its involvement was later demonstrated in multicentric Castleman disease and in primary lymphoma effusion lymphoma. These diseases arise almost exclusively in immunocompromised patients, mostly in association with HIV infection. Apart from Kaposi's sarcoma, combined antiretroviral therapy does not seem to have reduced the incidence of these diseases, which remain rare. In these three diseases, pulmonary involvement is common and may be the presenting feature. Kaposi's sarcoma of the lung is usually asymptomatic but may require specific therapy. Pulmonary involvement is mostly associated with cutaneous disease. Patients with Castleman disease typically present with fever and lymphadenopathy, associated with interstitial lung disease without opportunistic infection. Patients with primary lymphoma effusion presents with fever and an exudative lymphocytic pleural effusion, without a pleural mass on the CT-scan. Rapid diagnosis of these conditions avoids unnecessary invasive examinations and leads to prompt specific treatment. | {
"pile_set_name": "PubMed Abstracts"
} |
Isolation of soluble tyrosinase from human melanoma cells.
In the human melanoma cell tyrosinase exists in a membraneous and a soluble form. The membraneous enzyme has an N-terminal amino acid sequence identical to that predicted from a human c-DNA clone by Kwon et al.. The soluble form has now been isolated by a technique mainly based on the trypsin resistence of the enzyme and the use of hydrophobic interaction chromatography. The specific dopa oxidase activity of the soluble enzyme was 300 mumol/min x mg protein. On isoelectric focusing the enzyme was found in at least ten bands, pI between 3.8-4.6. The molecular weight was found to be 53,000 D. The N-terminal amino acid sequence was the same as that found in the membrane bound form of the enzyme, i.e. the protein maps at the c-albino locus. | {
"pile_set_name": "PubMed Abstracts"
} |
Synergistic action of a plant rhamnogalacturonan enhancing antitumor cytotoxicity of human natural killer and lymphokine-activated killer cells: chemical specificity of target cell recognition.
The spontaneous natural killer (NK) and lymphokine-activated killer (LAK) cytotoxicity of highly purified CD56+CD3- NK cells (90 to 95%) against NK-sensitive and NK-insensitive target cells was drastically enhanced when a rhamnogalacturonan contained in a commercially available Viscum album extract was present during 4-h cytotoxicity assays. This enhancement correlated strictly with an increased formation of NK cell or LAK cell/tumor cell conjugate formation. Information on the chemical specificity of NK cell and LAK cell interaction with target cells and with the rhamnogalacturonan was obtained from inhibition studies. The most efficient inhibitors (100% inhibition at 5 mg/ml) were acetylated D-mannose and acetylated L-mannonic acid gamma-lactone. They specifically inhibited in a dose-dependent manner: (a) the cytotoxicity of NK cells against K562 cells and the formation of NK cell/K562 cell conjugates; (b) the cytotoxicity of LAK cells against K562 cells and Daudi cells as well as the formation of LAK cell/K562 cell and of LAK cell/Daudi cell conjugates; and (c) the synergistic effects of the rhamnogalacturonan in the cytotoxicity assays and the target cell-conjugate formation assays with NK cells and LAK cells. The inhibitory effects observed after pretreatment of NK cells or LAK cells with acetylated mannose were completely reversible, but that obtained with acetylated mannonic acid gamma-lactone was only partly reversible, and the degree of reversibility depended on the inhibitor concentration applied during pretreatment. Nonacetylated mannose or mannose derivatives up to concentrations of 20 mmol showed no inhibitory effects. A mechanistic model representing the interaction of NK cells and LAK cells with target cells and with rhamnogalacturonan is proposed. | {
"pile_set_name": "PubMed Abstracts"
} |
Biochemical assessment of bone disease in multiple myeloma.
In patients with multiple myeloma, with moderate and severe bone disease, the urinary hydroxyproline excretion was disproportionately elevated with respect to the activity of bone isoenzyme of alkaline phosphatase when compared with the relationship between the variables observed in 58 age- and sex-matched controls and in 50 healthy young subjects. A significant positive correlation was found between urinary hydroxyproline excretion and the clinical variables related to the extent of bone involvement in multiple myeloma (X-rays, patient's performance status, anaemia). In 9 out of 13 patients with moderate and severe bone disease the chemotherapy-induced remission was associated with a significant (p less than 0.05) rise in the activity of bone isoenzyme of alkaline phosphatase and decrease (p less than 0.005) in urinary hydroxyproline excretion. In successfully treated patients, the relationship between the biochemical variables indicated increased but proportionate extents of whole-body rates of bone formation and resorption. This was not the case in patients in whom no chemotherapy-induced remission was noted. The simultaneous evaluation of the activity of bone isoenzyme of serum alkaline phosphatase and urinary excretion of hydroxyproline improves the assessment of bone involvement in multiple myeloma and the efficacy of treatment. | {
"pile_set_name": "PubMed Abstracts"
} |
Influence of UGT1A8 and UGT2B7 genetic polymorphisms on mycophenolic acid pharmacokinetics in Japanese renal transplant recipients.
UGT1A8 and UGT2B7 are important uridine diphosphate-glucuronosyltransferase isoforms for the glucuronidation of mycophenolic acid (MPA). The aim of this investigation was to elucidate MPA pharmacokinetics in UGT1A8 and UGT2B7 genotypes in Japanese renal transplant recipients. Seventy-two recipients received repeated doses of mycophenolate mofetil and tacrolimus. On day 28 after renal transplantation, plasma MPA concentrations were measured for the next 24 h using high-performance liquid chromatography. UGT1A8*2 (A(173)G) and UGT2B7*2 (Y(268)) were detected using a PCR-RFLP-based procedure. There were no significant differences in daytime and nighttime pharmacokinetics of MPA between UGT1A8 or UGT2B7 genotypes. The mean daytime dose-adjusted AUC(0-12) of MPA in UGT1A8*1/*1, *1/*2 and *2/*2 were 2.47, 2.33 and 2.57 ng.h/ml/mg/kg (P = 0.7711), and the mean nighttime AUC(0-12) were 2.15, 2.00 and 2.08 ng.h/ml/mg/kg (P = 0.4656). The mean daytime and nighttime dose-adjusted AUC(0-12) of MPA in UGT2B7*1/*1, *1/*2 and *2/*2 were 2.61, 2.24 and 2.03 ng.h/ml/mg/kg and 2.18, 1.94, and 1.45 ng.h/ml/mg/kg, respectively (P = 0.3475 and 0.2575). The mean nighttime C(max), t(max), and AUC(6-12)/AUC(0-12) ratio (enterohepatic circulation and recirculation ratio) of MPA in all UGT1A8 and UGT2B7 genotypes were lower, longer, and higher, respectively, than the daytime values. Both UGT1A8 and UGT2B7 allelic variants seem not to affect Japanese interindividual variability for plasma MPA concentration. Regardless of UGT1A8 and UGT2B7 genetic polymorphisms, the absorption of MPA through enterohepatic recirculation is higher at night. | {
"pile_set_name": "PubMed Abstracts"
} |
[Karyosphere capsule in Tribolium castaneum oocytes].
Structure and composition of the karyosphere (karyosome) capsule were studied in the oocytes of a laboratory insect, Tribolium castaneum, with the use of electron microscopy and immunoelectron cytochemistry. Basing on the study of nuclear structure dynamics, we distinguished 8 stages that characterize the period of oocyte growth. At the diplotene stage, T. castaneum oocyte chromosomes conjoin early into a compact karyosphere, but a significant chromatin condensation does not occur. The process of karyosphere formation is accompanied by the development of an extensive extrachromosome capsule surrounding chromatin. The capsule consists of a material of different morphological types. Significant molecular components of the T. castaneum karyosphere capsule are represented by the proteins of nuclear matrix including F-actin and lamin B. Besides the structural proteins, the Sm proteins of small nuclear (sn) RNPs and mature 2,2,7-trimethyl guanosine (TMG) 5'-capped snRNAs are revealed immunocytochemically in the karyosphere capsule. The obtained data can form a basis for further expansion of ideas on the functions of the karyosphere capsule as a specialized extrachromosomal nuclear domain of the oocytes. We believe that the T. castaneum karyosphere capsule plays not only a structural role, but may be involved directly in the processes related to gene expression. | {
"pile_set_name": "PubMed Abstracts"
} |
Comparative analysis of extra-nodal NK/T-cell lymphoma and peripheral T-cell lymphoma: significant differences in clinical characteristics and prognosis.
We aimed to compare the frequencies, clinical characteristics, and prognostic factors of peripheral T-cell lymphoma (PTCL) vs. extra-nodal natural killer (NK)/T-cell lymphoma and to characterize the subtypes of extra-nodal NK/T-cell lymphoma. We reviewed 97 consecutive patients with PTCL and extra-nodal NKT lymphoma from 2000 to 2006. During this period, a total of 780 patients with malignant lymphomas were treated in our center. The diagnostic criteria used were based on the WHO classification system of malignant lymphomas. Extra-nodal-NK/T-cell lymphoma and PTCL comprised 5.0% (39/780) and 7.4% (58/780) of all cases. Of the PTCL cases, histology was PTCL-NOS in 25, anaplastic large cell in 11, angioimmunoblastic T cell in 18 and other subtypes in four patients. Compared with PTCL, extra-nodal NK/T-cell lymphoma was associated with a significantly inferior rates of complete remission (33% vs. 53%, P = 0.05) and 3 yr overall survival (29.5% vs. 47.5%, P = 0.003). On multivariate analysis, extra-nodal NK/T-cell histology was independently associated with decreased survival. Further analysis into this subtype showed the nasal variant (n = 25) differed significantly from extra-nasal variant (n = 14) in terms of stage at presentation (stages III/IV, 36% vs. 79%), international prognostic index scores (high intermediate or high IPI scores, 24% vs. 64%), complete remission rates (48% vs. 7%), and median survival (10 months vs. 1 month, P < 0.0001). Extra-nodal NK/T-cell lymphoma was associated with a poorer prognosis compared with PTCL and is likely to comprise two distinct variants with different clinical behavior and prognosis. | {
"pile_set_name": "PubMed Abstracts"
} |
Adhesion of different bacterial strains to low-temperature plasma-treated sutures.
In this study, five different bacteria with their different strains were isolated and characterized. Contact angles were measured by a captive-bubble technique. Surface-free energies were calculated from the contact angles. Hydrophobicities also were evaluated by rho-xylene adhesion. The zeta potentials and surface charges of the bacteria were obtained. The contact angles of the gram-positive bacteria and gram-negative bacteria were within the range of 48 degrees-69 degrees and 43.5 degrees-55 degrees, respectively, while corresponding surface-free energies were in the limits of 45.4-51.6 erg/cm-2 and 51.7-61.8 erg/cm-2, respectively. The rho-xylene adhesions were parallel to hydrophobicities defined by contact angles, and 32.2-80.3% and 2.3-36.6% for the gram-positive bacteria and gram-negative bacteria, respectively. The zeta potentials for these bacteria were from -650.2 to +17.5 mV and from -159.6 to -6.0 mV, respectively. Most of the bacteria were negatively charged, except the CNS-2 and CPS-1 strains. In the second part of the study, attachment of these bacteria to Vicryl sutures and their DMAEMA and AAc plasma-treated forms were investigated. Hydrophobic bacteria attached more to hydrophobic Vicryl sutures. Both plasma treatments caused significant drops in bacterial attachment in most cases. Effects of AAc plasma treatment were more pronounced. | {
"pile_set_name": "PubMed Abstracts"
} |
Fetal skeletal computed tomography: when? How? Why?
To study the additional role of fetal skeletal computed tomography in suspected prenatal bone abnormalities. Two centers included in a retrospective study all fetuses who benefited from skeletal computed tomography for a suspected constitutional bone disease or focal dysostosis. A total of 198 patients were included. CT was performed in 112 patients (56%) for an isolated short femur below the third percentile (group A), in 15 patients (8%) for bowed or fractured femur (group B), in 23 patients (12%) for biometric discrepancy between a short femur and increased head circumference (group C) and in 48 patients (24%) for suspected focal dysostosis (group D). CT was interpreted as normal in 126 cases (64%), i.e. 87% in group A, 0% in group B, 65% in group C and 25% in group D. When including only cases with postnatal or postmortem clinical and/or radiological confirmation was available, CT provided additional and/or more accurate information than ultrasound in 20% of cases in group A, 66% in group B, 30% in group C and 72% in group D. Sixty-seven percent of patients in whom CT was interpreted as normal were lost to follow-up. In isolated short femur, fetal skeletal CT is normal in the great majority of cases although protocolized follow-up of these babies is absolutely compulsory, as a large proportion is lost to follow-up. Fetal skeletal CT can confirm or improve imaging for the suspected diagnosis in suspected focal dysostosis or constitutional bone disease. | {
"pile_set_name": "PubMed Abstracts"
} |
EUS training in a live pig model: does it improve echo endoscope hands-on and trainee competence?
The learning curve for endoscopic ultrasonography (EUS) is known to be difficult, especially in the field of pancreatic and biliary diseases. The aim of this study was to assess the impact of a live pig model developed for EUS credentialing in France. A total of 17 trainees obtained hands-on EUS experience using a live pig model. Trainees were asked to visualize anatomical structures, to carry out fine-needle aspiration (FNA) on lymph nodes in the liver hilum, and to perform celiac neurolysis. Assessment of the FNA procedure or celiac neurolysis included measurement of time (seconds), evaluation of the precision of the puncture (mm), and existence of technical errors. A significant improvement between a pre-test and post-test was observed for diagnostic procedures in the following anatomical areas: splenic mesenteric vein, vena cava, splenic mesenteric artery, celiac tree, pancreatic gland, and bile duct. For lymph node FNA, a significant improvement was observed in the duration of the procedure (84 seconds vs. 60 seconds; P = 0.01), and precision (4.2 mm vs. 1.8 mm; P = 0.009), but not for the rate of technical error (29% vs. 6%; not significant [n. s.]). For celiac neurolysis, a significant improvement was observed in procedure time (150 seconds vs. 84 seconds; P = 0.003), but not in the rate of technical error (6% vs. 6%; n. s.) or precision (4.2 mm vs. 2.8 mm; n. s.). Teaching EUS with a live pig model significantly increased competence in diagnostic procedures with regard to visualizing anatomical structures, performance of FNA and, to a lesser extent, EUS-guided celiac neurolysis. | {
"pile_set_name": "PubMed Abstracts"
} |
Racial differences in the patterns of preterm delivery in central North Carolina, USA.
In order to assess racial differences in rates of idiopathic preterm labour, preterm premature rupture of membranes, and medically indicated preterm delivery, the authors analysed data on 388 preterm (< 37 completed weeks of gestation) births (7.9% of all births) occurring between 1 September 1988 and 31 August 1989, in three central North Carolina counties. The crude relative risk (RR) of preterm birth among black women compared with white women was 2.6 [95% confidence interval (CI) 2.1, 3.1]. With adjustment for age, gravidity, marital status, education, and county of residence, the estimated relative risk for black women compared with white women was 2.1 (95% CI 1.1, 4.1) for medically indicated preterm delivery, 1.6 (95% CI 1.1, 2.3) for preterm birth as a result of preterm labour, and 1.9 (95% CI 1.2, 3.1) for preterm premature rupture of membranes. Compared with white women, black women were at the highest risk of a preterm birth before 34 weeks of gestation (RR = 2.9; 95% CI 1.8, 4.7). The risk of medically indicated preterm delivery at 36 weeks was considerably higher for black women than for white women (RR = 3.4; 95% CI 1.1, 10.2). For a better understanding and ultimately a reduction of the risk for preterm delivery among black women, investigation of specific aetiological pathways and gestational age groups may be required. | {
"pile_set_name": "PubMed Abstracts"
} |
A study of psychiatric morbidity in patients with Huntington's disease, their relatives, and controls. Admissions to psychiatric hospitals in Denmark from 1969 to 1991.
Psychiatric morbidity among 74 non-affected first-degree relatives and 93 non-affected second-degree relatives of patients with Huntington's disease (HD) was compared with that of 37 patients with HD and with matched control groups. Due to specific age criteria, the first-degree relatives were at decreased risk and the second-degree relatives at negligible risk of being carriers of the gene for HD. Information on admissions to departments of psychiatry and diagnoses at discharge were obtained for all subjects from a nationwide central register. Psychiatric morbidity was no greater among relatives than among controls, whereas HD patients had significantly more admissions and psychiatric diagnoses than relatives. Growing up with a risk of developing HD does not itself increase the risk of developing psychiatric illness resulting in hospital admission. Severe psychiatric disorders in HD patients were thus most likely to be aetiologically related to the disease process, possibly through a genetic mechanism. | {
"pile_set_name": "PubMed Abstracts"
} |
Expanding the roles of hospitalist physicians to include public health.
Several years after the inception of the hospitalist movement, hospitalist roles have evolved in breadth and sophistication. Although public health is not formally recognized or previously described as an arena for hospitalists, hospitalists are often engaged in public health practice. This article attempts to alert hospitalists to the potential to make contributions to the field of public health and defines the public health skills that can positively affect the lives of their patients and the communities they serve. In a public health role, hospitalists may improve the quality of inpatient care. This article reviews how public health and hospital-based practices have already intersected and proposes further development within this discipline. In our ever-changing health care system, hospitalists play key roles in the central public health domains of assessment, assurance, and policy development. Insightful hospitalists will recognize and embrace these responsibilities in caring for patients and society. | {
"pile_set_name": "PubMed Abstracts"
} |
Analysis of two distinct single-stranded DNA binding sites on the recA nucleoprotein filament.
The binding stoichiometry of Escherichia coli recA protein to single-stranded DNA (ssDNA) determined by two separate assays differs by a factor of 2.2-2.4. Using the fluorescence of etheno-DNA (epsilon DNA), a chemically modified ssDNA, the stoichiometry was found to be 7.0 +/- 0.6 bases/recA protein monomer in a nucleo-protein filament. Using a competition assay, a similar stoichiometry, 7.5 bases/recA, is found for unmodified poly(dT). Using the DNA-dependent ATPase of recA, which monitors bound protein rather than bound DNA, we find that each recA monomer needs to bind only 3.1 +/- 0.5 bases to fully activate the ATPase. The difference in site size determined by the two assays indicates that there are two DNA binding sites with differential effects on ATPase activation. When recA protein is mixed with ssDNA at a ratio of 7 bases/recA or greater, the complex that forms contains 7 bases/recA and acts as a kinetic trap for the ssDNA. Upon further addition of recA protein, no additional ATPase activity is observed. If, on the other hand, the ssDNA is initially mixed with excess recA (at a ratio of 3-3.5 bases/recA or less) the ATPase activity is twice as high. Analysis of the binding curves suggests that the first DNA strand binds recA to form a filament with a stoichiometry of 3-3.5 bases/protein monomer. The ATPase activity of recA is completely active in this complex. A second strand of DNA can then be bound to this filament yielding a final stoichiometry of approximately 7 bases/protein monomer. The presence of this second strand neither enhances nor inhibits ATP hydrolysis. This ternary complex may mimic the structures formed by recA in searching for homologous DNA sequences and/or in the strand exchange reaction. | {
"pile_set_name": "PubMed Abstracts"
} |
Adventitious shoot production from calloid cultures of banana.
Isolated tips (approx. 2 mm long) from aseptic, multiplying shoot cultures of the triploid dessert banana clone 'Highgate' were tested for their morphogenetic responsiveness to hormone treatments on semisolid media. Medium containing Murashige and Skoog (1962) salts, p-chlorophenoxyacetic acid, and kinetin produced a compact calloid mass. Protuberances disclosed by SEM as rounded, button-shaped, and pointed outgrowths resembling fasciated shoots were formed in profusion. Sections showed many meristematic regions, some associated with distinct leaf primordia. Formation and growth of successive leaves yielded small, elongated, adventitious shoots with constricted bases. Transferral to a basal MS medium with 1 mg/l 1-naphthaleneacetic acid (NAA) led to the formation of rooted plantlets. | {
"pile_set_name": "PubMed Abstracts"
} |
Pathology associated memory deficits in Swedish mutant genome-based amyloid precursor protein transgenic mice.
To gain insight into the relationship between pathological alterations and memory deficits observed in Alzheimer's disease (AD), a number of amyloid precursor protein (APP) transgenic animal models have been generated containing familial AD mutations. The most commonly utilized method involves a cDNA-based approach, utilizing heterologous promoters to drive expression of specific APP isoforms. As a result of the assumptions inherent in the design of each model, the different cDNA-based transgenic mouse models have revealed different relationships between the biochemical, pathological and behavioral alterations observed in these models. Here we provide further characterization of a genomic-based, amyloid precursor protein yeast artificial chromosome transgenic mouse model of AD, R1.40, that makes few assumptions regarding disease pathogenesis to study the relationship between brain pathology and altered behavior. Aged R1.40 transgenic and control mice were tested for learning and memory in the Morris water maze and for working memory in the Y maze. Results from the water maze demonstrated intact learning in the both control and R1.40 mice, but impairments in the long-term retention of this information in the transgenic mice, but not controls. Interestingly, however, long-term memory deficits did not correlate with the presence of Abeta deposits within the group of animals examined. By contrast, age-related working memory impairments were also observed in the Y maze in the R1.40 mice, and these deficits correlated with the presence of Abeta deposits. Our results demonstrate unique behavioral alterations in the R1.40 mouse model of AD that are likely both dependent and independent of Abeta deposition. | {
"pile_set_name": "PubMed Abstracts"
} |
Randomised clinical trial: the safety and efficacy of long-acting octreotide in patients with portal hypertension.
It remains unclear whether a long-acting preparation of octreotide (Sandostatin LAR) can be safely used for portal hypertension in patients with compensated cirrhosis. To determine the safety and efficacy of LAR among patients with Child Pugh Class A or B cirrhosis and small oesophageal varices. A randomised, double-blind, placebo-controlled study was conducted in 39 patients with cirrhosis and small oesophageal varices. Safety was based on frequency and severity of adverse events. Efficacy was determined by hepatic vein pressure gradient (HVPG) measured at baseline and day 84 following administration of LAR 10 mg (n = 15), 30 mg (n = 10) or saline (n = 14). Fasting and postprandial portal blood flow (PBF), superior mesenteric artery pulsatility index (SMA-PI), glucagon and octreotide levels were measured. An intention-to-treat analysis was performed. Four patients in the LAR 30 group (40%) withdrew from the study due to serious adverse events. No patient in the LAR 10 or control group had serious adverse events. There was no statistically significant decrease between HVPG at day 84 and baseline with LAR 30 mg (11.8 ± 2.3 mmHg vs. 14.1 ± 3.2), LAR 10 mg (15.3 ± 4.8 mmHg vs. 15.1 ± 3.8), or saline (13.3 ± 3.8 mmHg vs. 15.1 ± 4.3) (P = 0.26). Neither PBF, SMA-PI nor plasma glucagon levels were significantly decreased from baseline (P = 0.56). The absence of significant haemodynamic benefit, as well as the high frequency of severe adverse events associated with use of LAR, do not support the use of this agent in the treatment of portal hypertension. | {
"pile_set_name": "PubMed Abstracts"
} |
[Effects of aging on vestibular evoked myogenic potential].
The purpose of this study is to assess the effects of aging on air-conducted sound elicited cervical vestibular evoked myogenic potential (cVEMP) and ocular vestibular evoked myogenic potential(oVEMP) in normal people. Sixty normal subjects were recruited for the vestibular evoked myogenic potential examination. Among them, 20-40 year-old group was setted as the first group, 41-60 year-old group was setted as the second group, > 60-year-old group was setted as the third group. Each group included 20 normal subjects. SPSS 16.0 software was used to analyze the data. As the age growing, P1 latency prolonged, N1-P1 amplitude decreased, and amplitude asymmetric ratio increased in cVEMP; N1 latency prolonged, P1-N1 amplitude decreased, and amplitude asymmetric ratio increased in oVEMP. With the age growing, the time of otolithic organ input pathways prolonged, the function of otolithic organ decreased and the decrease level may be asymeetrical on both sides. | {
"pile_set_name": "PubMed Abstracts"
} |
A new set of positive/negative selectable markers for mammalian cells.
Five new positive and negative selectable markers were created for use in mammalian cells. Their negative selectabilities are based on the Thymidine kinase (Tk) gene of Herpes Simplex virus (HSV) or the Cytidine deaminase (codA) gene of E. coli. The markers can be selected positively by their ability to induce either Hygromycin (Hyg), neomycin (neo), puromycin (PAC) or Blasticidin S (BlaS) resistance. With these markers, two complete sets of markergenes are available that induce independent negative selectable phenotypes. | {
"pile_set_name": "PubMed Abstracts"
} |
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