A survey of Ambulance Victoria employees has found widespread bullying and morale problems.

The Victorian Opposition says a survey of 880 ambulance employees found one in three had been bullied and two-thirds of respondents were considering leaving because of low morale.

Victoria Labor's Health Parliamentary Secretary Wade Noonan says Ambulance Victoria is in crisis.

"There needs to be an acknowledgment that these sorts of results cannot continue," he said.

"The Government has to come out strongly and say that they are prepared to tackle the problems of workplace stress and bullying among our paramedics and Ambulance Victoria staff.

"Unless they actually acknowledge the problems, there's little chance we'll see any change."

Ambulance Victoria chief executive Greg Sasella says many respondents have interpreted things like rostering and procedural changes as bullying.

"New systems of rostering and dispatching and so forth, some people interpret that as bullying," he said.

"Delays at hospitals are very frustrating for people. We try and manage that tightly and they're interpreting that as bullying.

"Our attrition rate, people leaving the service, is less than 3 per cent."

The union representing Victorian ambulance workers says it disagrees with management's explanation.

Steve McGhie from the Ambulance Employee's Association says he was not surprised by the survey's findings.

"These issues have been brewing within the ambulance service for some time now and paramedics regularly inform the union of their concerns, the low morale, their consideration at leaving the job," he said.

"We've been signalling this to Ambulance Victoria and the State Government for quite some time now and unfortunately it hasn't been addressed."