Calcium levels during schizont rupture and erythrocyte invasion by P. falciparum merozoites. P. falciparum late stage schizonts were labeled with Fluo-4AM and added to erythrocytes to allow reinvasion. Both DIC and fluorescence images were acquired simultaneously on a Zeiss LSM510 confocal microscope at 2 second intervals with a 63×, 1.4 NA lens, using 488 nm excitation at low power to minimize bleaching and cell damage. A merge of the DIC and fluorescence images is shown.
Singh S, Alam M, Pal-Bhowmick I, Brzostowski J, Chitnis C
2010-02
<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816683/bin/ppat.1000746.s016.mov">Video S3</a> from <cite style="font-style:normal">Singh S, Alam M, Pal-Bhowmick I, Brzostowski J, Chitnis C (2010). "<a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816683">Distinct External Signals Trigger Sequential Release of Apical Organelles during Erythrocyte Invasion by Malaria Parasites</a>". <i>PLOS Pathogens</i>. <a href="https://en.wikipedia.org/wiki/Digital_object_identifier" class="extiw" title="w:Digital object identifier">DOI</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1371/journal.ppat.1000746">10.1371/journal.ppat.1000746</a>. <a href="https://en.wikipedia.org/wiki/PMID" class="extiw" title="w:PMID">PMID</a> <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pubmed/20140184">20140184</a>. <a href="https://en.wikipedia.org/wiki/PubMed_Central" class="extiw" title="w:PubMed Central">PMC</a>: <a rel="nofollow" class="external text" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2816683">2816683</a>.</cite>