<ol><li>Column loaded with silica/column medium</li>
<li>Eluting solvent added to compact silica layer and to remove air bubbles</li>
<li>Purple mixture as a thin layer is added to top of silica layer</li>
<li>Eluting solvent added and eluted (purple layer separates into a red and blue layer)</li>
<li>Eluting solvent added and eluted (red and blue layers separate further)</li>
<li>Red layer collected (the faster moving layer)</li>
<li>Blue layer collected (the slower moving layer)</li>
<li>No more compounds are eluted, process ended</li></ol>
quantockgoblin
<span class="int-own-work" lang="en">Own work</span>