western blotting之tbst配法
0.1% tbst?
0.05% tbst?
讓戰將來回答~~

圖片參考：http://www.molecularstation.com/images/western-blot.gif

 0.05%tbst
1. pour 3 l of purified water into a 4-l beaker and begin stirring.
2. measure 400ml 10x tbs in a 500-ml graduated cylinder and add to water.
3. add bsa and 2ml tween 20 to the beaker. rinse all of the tween 20 from the pipette tip into the solution.
4. transfer solution to a graduated cylinder and adjust to final volume 4l with purified water.
5. return solution to beaker and stir until thoroughly mixed.
以上是以 4l 為配置體積, 若你用不到那麼多可依比例減少配置體積, 而 2%bsa 的配法則依此要領, 將你們實驗室中用的 bsa 加入即可, 因為不知道你們是用 powder 還是 liquid 的 bsa~

圖片參考：http://www1.qiagen.com/products/genesilencing/allstarrnaicontrols/images/western.jpg

希望以上有幫到您~~
?answer :




step 1 of 6:


given, in a sample of 20 weld failures, 85% of them occur in the weld metal itself, and the remaining 15% occur in the base metal.


let x follows binomial distribution with probability mass function is




p(x: n,p) = (1 - p , x = 0, 1, 2, ….., n.


where,
n = sample size
 = 20
x = random variable
p = probability of success
 = 15% 
 = 0.15
q = 1 - p (probability of failure)


mean of the binomial distribution is 


 = np


variance of the binomial distribution is 


 = npq


\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_




step 2 of 6:




1. the claim is to find the probability that exactly five of them are base metal failures


we have to find p(x=5) 


p(x: n,p) = (1 - p , x = 0, 1, 2, ….., n.


 where, n = 20, p = 0.15 and x = 5


p(x = 5) = (1 - 0.15


 = (15504) (0.0000759) (0.087354)


 = 0.1028


hence, the probability that exactly five of them are base metal failures is 0.1028.


\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_




step 3 of 6:




1. the claim is to find the probability that fewer than four of them are base metal failures


we have to find p(x < 4) = p(x = 0) + p( x = 1) + p(x = 2) + p(x = 3) 






p(x: n,p) = (1 - p , x = 0, 1, 2, ….., n.


 where, n = 20, p = 0.15 and x = 0, 1, 2 and 3


p(x < 4) = (1 - 0.15+ (1 - 0.15+


 (1 - 0.15+ (1 - 0.15


 = (1) (1) (0.03876) + (20) (0.15) (0.0456) +
 (190) (0.0225) (0.053646) + (1140) ( 0.003375) (0.0631134)


 = 0.03876 + 0.1368 + 0.2293 + 0.2428


 = 0.6477


hence, the probability that fewer than four of them are base metal failures 0.6477


\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_




step 4 of 6:




c) the claim is to find the probability that none of them are base metal failures.


we have to find p(x=0) 


p(x: n,p) = (1 - p , x = 0, 1, 2, ….., n.


 where, n = 20, p = 0.15 and x = 0


p(x = 0) = (1 - 0.15


 = (1) (1) (0.03876) 


 = 0.03876


hence, the probability that none of them are base metal failures is 0.03876


\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_




step 5 of 6:




d) the claim is to find the mean number of base metal failures..


 mean of the binomial distribution is = np


where, n = 20, and p = 0.15


substitute these values in 


then, = (20) (0.15)


 = 3


hence, the mean number of of base metal failures is 3.


\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_




step 6 of 6:




e) the claim is to find the standard deviation of the number of base metal failures..


standard deviation of the binomial distribution is 


where, n = 20, p = 0.15 and q = 1 - p


 = 1 - 0.15


 = 0.85


substitute these values in 


then, 


 


 = 1.596


hence, the standard deviation of the number of base metal failures is 1.596.


\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_\_ 0.5 answer: `1.00` no minimum, there was a annual review part on tesla channel recently and off the numbers i saw without promotion, increments varied between 2.5 to 10 percent Tungsten, also known as wolfram, is a hard, rare metal that is found naturally only in chemical compounds. The free element is remarkable for its robustness, especially the fact that it has the highest melting point of all the elements.  
Tungsten in pure form has the highest melting point (3422 °C, 6192 °F), lowest vapor pressure (at temperatures above 1650 °C, 3000 °F) and the highest tensile strength. Tungsten has the lowest coefficient of thermal expansion of any pure metal. The low thermal expansion and high melting point and tensile strength of tungsten originate from strong covalent bonds formed between tungsten atoms by the 5d electrons. Alloying small quantities of tungsten with steel greatly increases its toughness. hii..

according to me,the cut off will be around 90-95 marks for general category,50-55 marks for obc category,35-40 marks for sc category and 25-30 marks for st category.so it is difficult to be predict now.

please see the table below for previous years trends:-

 

jee main cut off trends (2013-2017)

| s.no. | category | 2017 | 2016 | 2015 | 2014 | 2013 |
| 1 | general | 81 | 100 | 105 | 115 | 113 |
| 2 | obc-ncl | 49 | 70 | 70 | 74 | 70 |
| 3 | sc | 32 | 52 | 50 | 53 | 50 |
| 4 | st | 27 | 48 | 44 | 47 | 45 | 0.32 = e-0.00012tln 0.32 = -0.00012t-1.139 ≈ -0.00012t9495.29 ≈ t since the solution is a buffer, we can use the henderson-hasselbalch equation to calculate the ratio of [so32-]/[hso3-] to have a ph of 7.39:ph=pka+logconjugate baseweak acidin the solution, identify the weak acid and the conjugate base. recall from the bronsted-lowry definition that an acid is a proton (h+) donor and the base is a proton (h+) acceptor.• nahso3 is a salt that will give hso3- when it dissociatesnahso3 → na+ + hso3-• na2so3 is a salt that will give so32- when it dissociatesna2so3 → 2 na+ + so32-•hso3- is a weak acid.          - concentration of hso3- = concentration of nahso3• when hso3- loses h+ to give a conjugate base, so32- is formedhso32- - h+ → so32-- concentration of so32- = concentration of na2so3      hso3-    +   h2o     →        so32-        +       h3o+(weak acid)    (base)      (conjugate base)  (conjugate acid)we can rewrite the henderson-hasselbalch equation as:ph=pka+logso32-hso3-calculate [so32-]/[hso3-] ratio:• ph = 7.39• pka = 7.19ph=pka+logso32-hso3-7.39=7.19+logso32-hso3-logso32-hso3-=7.39-7.19logso32-hso3-log=0.2loglog has a base of 10 and when you divide a number by log you will take 10 to the power of the numberso32-hso3-=100.2[so32-]/[hso3-] = 1.58the ratio [so32-]/[hso3-] is 1.58 105 mpa if the amount is very low. it is normal. dont worry