pls explain replication of dna and semiconservative nature of dna i detail - biology - molecular basis of inheritance
pls explain replication of dna and semiconservative nature of
dna i detail?
the process through which dna creates its own copies is known as
dna replication


at first, dna double helix starts unwinding in several points these
points are known as origin of replication

enzyme helicase helps in the process of unwinding by breaking the
h- bonds of the paired nucleotides to break apart the two templates
of dna

by the action of enzyme primase, a small primer
which is a small chain of rna by polymerization is formed on the
template at the point of origin of
replication

after unwinding up to a certain point, dna represents a y
â€“ shaped replication fork

the enzyme dna polymerase starts supplying
nucleotides that are complementary to the nucleotides of the dna
template in 5â€™ â€“
3â€™ direction

the synthesis of new strand will be in opposite direction as both
the strands run in antiparallel manner

the new strand that is formed on 3â€™
â€“ 5â€™ strand of template dna
is a continuous strand in 5â€™
â€“ 3â€™ direction and is known
as leading strand however the strand that is
formed on the other template of double helix are formed in
fragments and called lagging strand the small
fragments are called okazaki fragment

the fragments are joined by the joining enzyme called
ligase

during replication, dna polymerase automatically goes back to the
point of the strand where wrong base is formed and corrects it
(proof reading) before proceeding to further
replication



![](https://s3mn%20mnimgs%20com/img/shared/discuss_editlive/4139900/2013_09_18_20_19_24/npzgtlsr3923109580584032143%20jpg)
peculiarities of the dna structure: dna is a double helical structure having nucleotides. these nucleotides are made of deoxyribose sugar, phosphoric acid and nitrogenous bases such as adenine, thymine, cytosine and guanine. the nucleotide sequence of the dna molecule is called a gene. genes are present on dna. a type of gene is responsible for the synthesis of a particular protein. changes in the gene can alter the characteristics of the organisms. please see the above link to learn more. to ask any questions on the content covered in this tutorial please start a new topic within the forum. you may check this how to guide for reference - [how to use the enterprise dna support forum](https://forum.enterprisedna.co/t/how-to-use-the-enterprise-dna-support-forum/3951) please see the above video to learn more. to ask any questions on the content covered in this tutorial please start a new topic within the forum. you may check this how-to guide for reference - [how to use the enterprise dna support forum](https://forum.enterprisedna.co/t/how-to-use-the-enterprise-dna-support-forum/3951) http://en.wikipedia.org/wiki/genetically\_modified\_food **Principle of Insertional Inactivation-** When cloned DNA is inserted into a gene it inactivates its functioning. Sequence/ insert cloned into vectors within genes of antibiotic resistance, leads to loss of function.

**Steps**

1. Host cells (E. coli cells) with recombinant pBR322 plasmid are first plated on solid media (agarose containing) with the antibiotic ampicillin (assume that the insert has been ligated within the tetracycline resistance gene). Colonies from every single cell plate having the plasmid will develop overnight

2. A petri plate containing solid media with antibiotic tetracycline is kept carefully under aseptic conditions (Laminar flow hood)

3. A circular piece of velvet or velvet paper is aligned and pressed onto the colony containing ampicillin plate (master plate). With the same alignment it is pressed onto the tetracycline plate. Overnight only colonies not containing the insert will grow while due to insertional inactivation no colonies will grow which have the insert. The colonies which have the insert can easily be scored off by comparing the two plates.

![](https://www.sarthaks.com/?qa=blob&qa_blobid=14248038838146221627) complexion ka relation genes se hota....kesar ya haldi se nahi....kesar haldi se hota to bache piley paida hotey hybridisation: d2sp3magnetic character: paramagneticspin nature of complex: low-spin complex yeah, its an incomplete genetic code, having only half the required chromosomes needed to create a life. it also doesn't consume food and produce waste, it cannot itself reproduce, etc. its clearly and obviously, as long as your not an anti science person, not a human being but a fetus is (again so long as your not anti-science). [image][queries and resolvers](https://forums.fauna.com/t/queries-and-resolvers/597/3)

do you have any feedback on the difficulties i am having doing this and any suggestions as to how i should be approaching this problem?

perhaps there is something i’m clearly misunderstanding that can be addressed in a reference article or section of the documentat… what makes science and pseudoscience different is the way that they are meant to find out details about different things. there are some people who make use of pseudoscience when they would like to find evidence that will support the claims