 );
 
 -- Populates the prep_1 dynamic table
-INSERT INTO qiita.prep_1 (sample_id, BarcodeSequence, LIBRARY_CONSTRUCTION_PROTOCOL, LinkerPrimerSequence, TARGET_SUBFRAGMENT, target_gene, RUN_CENTER, RUN_PREFIX, RUN_DATE, EXPERIMENT_CENTER, EXPERIMENT_DESIGN_DESCRIPTION, EXPERIMENT_TITLE, PLATFORM, SAMP_SIZE, SEQUENCING_METH, illumina_technology, SAMPLE_CENTER, pcr_primers, STUDY_CENTER) VALUES
-	('SKB1.640202', 'GTCCGCAAGTTA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKB2.640194', 'CGTAGAGCTCTC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKB3.640195', 'CCTCTGAGAGCT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKB4.640189', 'CCTCGATGCAGT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKB5.640181', 'GCGGACTATTCA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKB6.640176', 'CGTGCACAATTG', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKB7.640196', 'CGGCCTAAGTTC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKB8.640193', 'AGCGCTCACATC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKB9.640200', 'TGGTTATGGCAC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD1.640179', 'CGAGGTTCTGAT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD2.640178', 'AACTCCTGTGGA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD3.640198', 'TAATGGTCGTAG', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD4.640185', 'TTGCACCGTCGA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD5.640186', 'TGCTACAGACGT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD6.640190', 'ATGGCCTGACTA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD7.640191', 'ACGCACATACAA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD8.640184', 'TGAGTGGTCTGT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKD9.640182', 'GATAGCACTCGT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM1.640183', 'TAGCGCGAACTT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM2.640199', 'CATACACGCACC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM3.640197', 'ACCTCAGTCAAG', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM4.640180', 'TCGACCAAACAC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM5.640177', 'CCACCCAGTAAC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM6.640187', 'ATATCGCGATGA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM7.640188', 'CGCCGGTAATCT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM8.640201', 'CCGATGCCTTGA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
-	('SKM9.640192', 'AGCAGGCACGAA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME');
+INSERT INTO qiita.prep_1 (sample_id, study_id, BarcodeSequence, LIBRARY_CONSTRUCTION_PROTOCOL, LinkerPrimerSequence, TARGET_SUBFRAGMENT, target_gene, RUN_CENTER, RUN_PREFIX, RUN_DATE, EXPERIMENT_CENTER, EXPERIMENT_DESIGN_DESCRIPTION, EXPERIMENT_TITLE, PLATFORM, SAMP_SIZE, SEQUENCING_METH, illumina_technology, SAMPLE_CENTER, pcr_primers, STUDY_CENTER) VALUES
+	('SKB1.640202', 1, 'GTCCGCAAGTTA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKB2.640194', 1, 'CGTAGAGCTCTC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKB3.640195', 1, 'CCTCTGAGAGCT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKB4.640189', 1, 'CCTCGATGCAGT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKB5.640181', 1, 'GCGGACTATTCA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKB6.640176', 1, 'CGTGCACAATTG', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKB7.640196', 1, 'CGGCCTAAGTTC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKB8.640193', 1, 'AGCGCTCACATC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKB9.640200', 1, 'TGGTTATGGCAC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD1.640179', 1, 'CGAGGTTCTGAT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD2.640178', 1, 'AACTCCTGTGGA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD3.640198', 1, 'TAATGGTCGTAG', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD4.640185', 1, 'TTGCACCGTCGA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD5.640186', 1, 'TGCTACAGACGT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD6.640190', 1, 'ATGGCCTGACTA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD7.640191', 1, 'ACGCACATACAA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD8.640184', 1, 'TGAGTGGTCTGT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKD9.640182', 1, 'GATAGCACTCGT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM1.640183', 1, 'TAGCGCGAACTT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM2.640199', 1, 'CATACACGCACC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM3.640197', 1, 'ACCTCAGTCAAG', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM4.640180', 1, 'TCGACCAAACAC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM5.640177', 1, 'CCACCCAGTAAC', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM6.640187', 1, 'ATATCGCGATGA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM7.640188', 1, 'CGCCGGTAATCT', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM8.640201', 1, 'CCGATGCCTTGA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME'),
+	('SKM9.640192', 1, 'AGCAGGCACGAA', 'This analysis was done as in Caporaso et al 2011 Genome research. The PCR primers (F515/R806) were developed against the V4 region of the 16S rRNA (both bacteria and archaea), which we determined would yield optimal community clustering with reads of this length using a procedure similar to that of ref. 15. [For reference, this primer pair amplifies the region 533_786 in the Escherichia coli strain 83972 sequence (greengenes accession no. prokMSA_id:470367).] The reverse PCR primer is barcoded with a 12-base error-correcting Golay code to facilitate multiplexing of up to 1,500 samples per lane, and both PCR primers contain sequencer adapter regions.', 'GTGCCAGCMGCCGCGGTAA', 'V4', '16S rRNA', 'ANL', 's_G1_L001_sequences', '8/1/12', 'ANL', 'micro biome of soil and rhizosphere of cannabis plants from CA', 'Cannabis Soil Microbiome', 'Illumina', '.25,g', 'Sequencing by synthesis', 'MiSeq', 'ANL', 'FWD:GTGCCAGCMGCCGCGGTAA; REV:GGACTACHVGGGTWTCTAAT', 'CCME');
 
 -- Insert preprocessed information for raw data 1
 INSERT INTO qiita.preprocessed_data (preprocessed_params_table, preprocessed_params_id, submitted_to_insdc_status, ebi_submission_accession, ebi_study_accession, data_type_id) VALUES ('preprocessed_sequence_illumina_params', 1, 'submitting', 'EBI123456-AA', 'EBI123456-BB', 2), ('preprocessed_sequence_illumina_params', 2, 'not submitted', NULL, NULL, 2);

INSERT INTO qiita.analysis_filepath (analysis_id, filepath_id, data_type_id) VALUES (1, 14, 2), (1, 15, NULL);

-- Attach samples to analysis
INSERT INTO qiita.analysis_sample (analysis_id, processed_data_id, sample_id) VALUES (1,1,'SKB8.640193'), (1,1,'SKD8.640184'), (1,1,'SKB7.640196'), (1,1,'SKM9.640192'), (1,1,'SKM4.640180'), (2,1,'SKB8.640193'), (2,1,'SKD8.640184'), (2,1,'SKB7.640196'), (2,1,'SKM3.640197');

--Share analysis with shared user
INSERT INTO qiita.analysis_users (analysis_id, email) VALUES (1, 'shared@foo.bar');

	('SKD1.640179', 'winter', 'n', '66 sand, 16.3 silt, 17.7 clay', '410658', 0.15, '3483', 'soil metagenome', 0.178, 114, 15, 1.51, 7.1, 0, 'ENVO:Temperate grasslands, savannas, and shrubland biome', 'GAZ:United States of America', 6.8, 'SKD1', 4.32, 'Diesel bulk', 'ENVO:plant-associated habitat');

-- Add the common prep info for study 1
INSERT INTO qiita.common_prep_info (raw_data_id, sample_id, study_id, center_name, center_project_name, emp_status_id) VALUES
	(1, 'SKB8.640193', 1, 'ANL', NULL, 1),
	(1, 'SKD8.640184', 1, 'ANL', NULL, 1),
	(1, 'SKB7.640196', 1, 'ANL', NULL, 1),
	(1, 'SKM9.640192', 1, 'ANL', NULL, 1),
	(1, 'SKM4.640180', 1, 'ANL', NULL, 1),
	(1, 'SKM5.640177', 1, 'ANL', NULL, 1),
	(1, 'SKB5.640181', 1, 'ANL', NULL, 1),
	(1, 'SKD6.640190', 1, 'ANL', NULL, 1),
	(1, 'SKB2.640194', 1, 'ANL', NULL, 1),
	(1, 'SKD2.640178', 1, 'ANL', NULL, 1),
	(1, 'SKM7.640188', 1, 'ANL', NULL, 1),
	(1, 'SKB1.640202', 1, 'ANL', NULL, 1),
	(1, 'SKD1.640179', 1, 'ANL', NULL, 1),
	(1, 'SKD3.640198', 1, 'ANL', NULL, 1),
	(1, 'SKM8.640201', 1, 'ANL', NULL, 1),
	(1, 'SKM2.640199', 1, 'ANL', NULL, 1),
	(1, 'SKB9.640200', 1, 'ANL', NULL, 1),
	(1, 'SKD5.640186', 1, 'ANL', NULL, 1),
	(1, 'SKM3.640197', 1, 'ANL', NULL, 1),
	(1, 'SKD9.640182', 1, 'ANL', NULL, 1),
	(1, 'SKB4.640189', 1, 'ANL', NULL, 1),
	(1, 'SKD7.640191', 1, 'ANL', NULL, 1),
	(1, 'SKM6.640187', 1, 'ANL', NULL, 1),
	(1, 'SKD4.640185', 1, 'ANL', NULL, 1),
	(1, 'SKB3.640195', 1, 'ANL', NULL, 1),
	(1, 'SKB6.640176', 1, 'ANL', NULL, 1),
	(1, 'SKM1.640183', 1, 'ANL', NULL, 1);

-- Add raw data prep columns
INSERT INTO qiita.raw_data_prep_columns (raw_data_id, column_name, column_type) VALUES
	(1, 'sample_id', 'varchar'),
	(1, 'study_id', 'biginy'),
	(1, 'BarcodeSequence', 'varchar'),
	(1, 'LIBRARY_CONSTRUCTION_PROTOCOL', 'varchar'),
	(1, 'LinkerPrimerSequence', 'varchar'),
