anchor.diff:                                      standardised protocols as described in IMPReSS (International Mouse Phenotyping Resource of Standardised Screens).</p>
                                   <p>Hearing capacity is assessed using an <a
                                           href="https://www.mousephenotype.org/impress/protocol/149/7">Auditory Brain Stem
  -                                    response (ABR)</a> protocol conducted
  +                                    response (ABR)</a> test conducted
                                       at 14 weeks of age. Hearing is assessed at five frequencies – 6kHz, 12kHz, 18kHz, 24kHz and 30kHz –
                                       as well as a broadband click stimulus.  Increased thresholds are indicative of abnormal hearing.
                                       Abnormalities in adult ear morphology are
  -                                    recorded as part of the <a href="http://www.mousephenotype.org/impress/protocol/186">Combined SHIRPA and Dysmorphology (CSD)</a> protocol and in the developing embryo during gross pathology examination.
  -                                    The CSD protocol includes response to a click box test (absence is indicative of a strong hearing deficit) and  visual inspection for behavioural signs that may indicate vestibular dysfunction e.g. head bobbing or circling.
  -                                    Full procedures details are provided in the list below.
  +                                    recorded as part of the <a href="http://www.mousephenotype.org/impress/protocol/186">Combined SHIRPA and Dysmorphology (CSD)</a> protocol, which 
  +                                    includes a response to a click box test (absence is indicative of a strong hearing deficit) and  visual inspection for behavioural signs that may indicate vestibular dysfunction e.g. head bobbing or circling.
                                   </p>
                                   </p>
   
  
positive.code:                                		52 genes are not previously associated with hearing loss and encompass a wide range of functions from structural proteins to transcription factors                               	
                                 	</li>
                                 	<li>
                                 		Among the novel candidate genes, Atp2b1 is expressed in the inner ear and Sema3f plays a role in sensory hair cell innervation in the cochlea
                                 	</li>
                                 	<li>
                                 		The IMPC has currently explored 15% of the mouse genome suggesting a minimum of 450 genes required for hearing function in the mammalian genome
                                 	
                                 	</li>
                                 	<li>
                                 		The IMPC will continue screening for hearing loss mutants in its second 5 year phase
                                 		
                                 	</li>
                                 	</ul>
                                 	
                                 	<h3>
                                 		Approach
                                 	</h3>
                                 		Response data from the auditory brainstem response (ABR) test was used – hearing at five frequencies, 6kHz, 12kHz, 18kHz, 24kHz and 30kHz, was measured.
  								<ul>
  								<li>
  									Control wildtype mice from each phenotypic centre included, matched for gender, age, phenotypic pipeline and metadata (e.g. instrument)
  
negative.code: 
                          <div class="section" id="manuscript">
                              <%--deafness manuscript --%>
                              <h2 class="title">IMPC Deafness Publication</h2>
                              <div class="inner">
                                 <h3>Hearing loss investigated in 3,006 knockout mouse lines</h3>
                                 <p><a href="http://em.rdcu.be/wf/click?upn=KP7O1RED-2BlD0F9LDqGVeSJ-2BYK13AOQwsehfu7fiqrAw-3D_rlS5NjXTpmOl66SHsP12rLQI7Hycd1QZjA7AhdQ2b6-2FS-2FPWAuljWGaoUUyQdBhNd5qzu1OSDRnDXa0UviCH1ywsoXX2HEGPW67FIlbNWZVB87NAsEeYQQ3F2GD8bvwBOk-2BPkbKRPqfrgHP4barsF-2BrLfjDnMD0fmPe675sK7phkS3L5CVy1mUUmp4mw-2Fgzbv1ueMSvy-2FUyo41KChqsXtnXKcvriwPUvjZxg6BOMnIFU-3D">Nature Communications</a></p>
  
                                 	<ul>
                                 	<li>
                                 		67 genes identified as candidate hearing loss genes
  