Any recommendation on my.cnf on aws.large instance? Any best recommendation variables for my.cnf on percona server on a high 256GB RAM and 32 CPU with 4000 IOPS?

How about below ones?

&gt; innodb_buffer_pool_size=180G 
&gt; innodb_buffer_pool_instances=16
&gt; innodb_flush_neighbors=0


any other recommendations on Mysql percona 5.6?
With that kind of AWS instance, you must be planning heavy writes.

I would add the following

innodb_log_buffer_size  = 32M
innodb_read_io_threads  = 16
innodb_write_io_threads = 16