If you want to express the largest amount of a certain or a few proteins using a plasmid, does it make sense for the antibiotic resistance gene to come after genes for proteins you intend to synthesize? So that the promoter and RBS/origin of replication would specifically come before the proteins intended to be synthesized and the bacterium would put less resources into its antibiotic resistance? But would that be canceled out by bacteria not surviving as much as if the antibiotic resistance gene is downstream of the other proteins? Or is it roughly the same in either order because they're both being synthesized anyway?