import sys

def parseMaps(goodMapFile, badMapFile):
	with open(goodMapFile) as gmf:
		goodMap = gmf.readlines()
		del goodMap[0]
	
	with open(badMapFile) as bmf:
		badMap = bmf.readlines()
		del badMap[0]
	
	bcMap = {}
	
	# create a map from the sample ID to the good barcode
	for line in goodMap:
		line = line.split('\t')
		bcMap[line[0]] = line[1]
		
	for line in badMap:
		line = line.split('\t')
		if line[0] in bcMap:
			bcMap[line[0]] = (line[1], bcMap[line[0]])
	
	return dict([t for t in bcMap.values() if isinstance(t, tuple)])


if __name__ == '__main__':
	goodMapFile = sys.argv[1]
	badMapFile = sys.argv[2]
	fastaFile = sys.argv[3]
	
	bcMap = parseMaps(goodMapFile, badMapFile)
	
	newFasta = open('new.fasta', 'w')
	
	with open(fastaFile) as f:
		for line in f:
			if line[0] == '>':
				header = line
				newFasta.write(header)
			else:
				# sequence info
				seq = line
				badCode = seq[:8]
				if badCode in bcMap:
					newFasta.write(bcMap[badCode] + seq[8:])
				else:
					print 'Mapping error: ' + header
	
	newFasta.close()
Create a new QIIME mapping file by replacing incorrect barcodes
Create a new QIIME mapping file by replacing incorrect barcodes