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## Control
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##Finding a suitable internal control
For an internal control an aptamer with emission at a different wavelength was needed. Based on the Paige et al, 2011 the aptamers were recreated, the fluorophores were synthesized and then tested at the fluorometer, Fluoro-Max-3. As seen in figure XX, the fluorescence enhancement was virtually non-existing for most of the aptamer-fluorophore complexes, except for the 17-3 & DMHBI complex, which showed an increase in fluorescence when compared to DMHBI, as the only one of the aptamer-fluorophore-complexes apart from Spinach and DFHBI. The fluorescence enhancement wasn’t as good as needed, therefore when the Mango-aptamer and fluorophore were published by Dolgosheina et. al. in August 2014, it was synthesized as fast as possible.

We expect the Mango-complex to have a fluorescence enhancement greater than the 17-3 complex, which will make this aptamer-complex more suitable for an internal control compared with the previously known aptamer-complexes. 


 (Graph of aptamers' efficiency)

###Orthogonality
The internal control and the Spinach constructs need to be orthogonal in two ways. The emission wavelengths must be different from one another, and the RNA aptamers should not be capable of binding each others fluorophores. Therefore, experiments were conducted to test if the Spinach aptamer can bind the Mango fluorophore or if the Mango aptamer can bind the DFHBI. If they don’t bind to the fluorophore of each other, the fluorescence signal will be specific and that will make Mango suitable for being the internal control in the sensor system.  

Put in all about controls
Put in all about controls
