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What is probate court? |
Below is a paragraph from an academic paper. Polish the writing to meet the academic style, improve the spelling, grammar, clarity, concision and overall readability. When necessary, rewrite the whole sentence.
1. Introduction
The parathyroid gland is of the latest discovered organs that typically located behind the thyroid. There are two pairs of parathyroid glands which are normally about the size of soy bean. To date, due to the difficulty in obtaining fresh biological specimens in human and even in animal model, little is known about this tiny but vital organ except that it monitors serum calcium levels and secretes parathyroid hormone (PTH) to regulate calcium and phosphorus metabolism. Some studies indicate parathyroid may also synthesis PTH related protein (PTHrP), chromogranin-A (CHGA), leptin and calcitriol using immunohistochemical approaches. However, the significance of these molecular in parathyroid gland still have not been fully elucidated. Our understanding of its cell composition, its specific molecular markers, specific receptor and its secretion spectrum are still limited.
In the literature, parathyroid parenchyma is mainly composed of two cell types according to its hematoxylin and eosin staining, known as chief and oxyphil cells. The chief cell was reported as the functional endocrine cells responsible for the secretion of PTH, because these cells showed high levels of CHGA, Calcium-sensing receptor (CaSR) and PTH. However, the significance of oxyphil cells has not been elucidated, this type of cells was also express CHGA, CaSR and PTH, but with larger cytoplasm and more eosinophilic due to a high mitochondrial content. The difference expression signature between the two types of cells is also highly controversy in the literature. Study suggested the oxyphil cells produce more PTHrP and calcitriol but less PTH using immunohistochemical approaches, while another study suggest oxyphil cell express higher levels of PTH and CaSR than chief cell and responsible for the age-related hypocalcemia. The difference between chief and oxyphil cells remain unclear. Furthermore, whether it exist other specialized endocrine cells subpopulations within parathyroid gland as we have found in many other endocrine glands need further investigation.
A transcriptome study has suggested the different expression signature between chief and oxyphil cells, however, this study was only based on chief and oxyphil adenomas samples, Moreover, the transcriptome of whole samples cannot reveal the specific expression profile of different cell population within tissues. More recently, a single-cell RNA sequencing study have provided the expression atlas of tumor environment of parathyroid adenomas samples. However, there still no data about the normal parathyroid gland. In addition, embryologic studies indicated that the upper and lower parathyroids begin to develop from different branchial pouches respectively, with the lower pair were origin with the thymus. Whether there are functional different between them remain to investigated. |
What are slithy toves? |
Medicine for bladder stones |
Somalia drone strike news today |
the cold can freeze your hands |
Придумай уровни сложности для моего DYOM проекта, назови их, опиши сколько здоровья у врагов, если ли оружие и броня:
Six years before Fort Carson was besieged by CEO Carl Pierce and his Panthers mercenaries, the town's biggest problem was the violent showdown between Bobby Big and The Fort Carson MC.
This five episode series explores Buck Jean's role in Bobby Big's business and in taking Fort Carson's drug market back from The Bikers. |
what is the value of a professional certification? |
Write a description for etsy, suitable for google seo, with asahigai text and tags. This simple yet elegant stone initial necklace is perfect for everyday wear. This minimalist piece can be worn layered or on its own. It is a unique and ideal jewellery for giving to a special someone or as a gift for yourself. Personalised silver initial necklace for you - Lover - Mom - Women - Girl, Gold Letter Pendant Birthday Gift for Mother, Name necklace |
ng/30 s are you familiar with these units ? |
Find HR generalists in Snyder, MO |
(Eggman’s Announcement meme) orochimaru Announcement |
Describe triple orbital resonance |
Carpathians mountains' and main wales mountains' Latin names |
african influences of tango music |
name 2 important people in the hampton roads battle |
Collection of Details of Korea-Japan Summit from韩国青瓦台网站（https://www1.president.go.kr/）
日本首相官邸网站（https://www.kantei.go.jp/）
韩国外交部网站（http://www.mofa.go.kr/eng/）
日本外务省网站（https://www.mofa.go.jp/） |
What is a small dice in culinary? |
which is the best hotel to lodge in Nodi (FIJI) that priorities budget , wifi, accessibility to tourist popular sites and comfort |
Let's say I time travelled back in time and Thomas Jefferson asked me what food people ate in the 21st century. How would I explain ramen to him? |
rephrase By SASAC’s requirements, Syngenta IPO can not be lower than 1x pre IPO P/B. The implied valuation is lower than comparable including Corteva and provides lots of upsides and leaves room for post-IPO share price performance |
Extract all the revision numbers from “mbox_short.txt”. For example:
New Revision: 39756
The revision number in this line is 39756. Store your result in a list called revs. Print out its length
and the first 5 values. |
Is it bad that I don't really care about historical accuracy or seriousness in a historical setting? |
Real life examples of informative prior and non informative prior |
Why defining an IT risk appetite is important |
Does the Xbox One really have 2 retail exclusives? |
Does Fairey |
what the different between ‘adopt’ and ‘adjust’ |
func MetricReadStatusMapper(sReference interface{}, dataOld string, dataNew string) string {
var sReferences []map[string]interface{}
json.Unmarshal([]byte(sReference.(string)), &sReferences)
userReadStatus := "true,true,true,true,true,true,true,true,true"
split := strings.Split(userReadStatus, ",")
for _, value := range sReferences{
key := ShrimpEstateMetricKey[value["key"].(string)]
statusDataOld := "good"
statusDataNew := "wary"
keyIndex := key.(int)
splitDataOld := strings.Split(dataOld, ",")[keyIndex]
for _, sReference := range sReferences {
if key == sReference["key"].(string) {
var reference []map[string]interface{}
byteReference, _ := json.Marshal(sReference["reference"])
json.Unmarshal(byteReference, &reference)
checker := false
for _, refValue := range reference {
var parameter []map[string]interface{}
byteParameter, _ := json.Marshal(refValue["parameter"])
json.Unmarshal(byteParameter, ¶meter)
for _, parValue := range parameter {
convRaw, _ := strconv.ParseFloat(sReference["raw_value"].(string), 64)
if !checker {
statusDataOld = refValue["status"].(string)
switch parValue["type"] {
case "greater-than":
conv, _ := strconv.ParseFloat(parValue["value"].(string), 64)
if conv < convRaw {
checker = true
break
}
case "lower-than":
conv, _ := strconv.ParseFloat(parValue["value"].(string), 64)
if conv > convRaw {
checker = true
break
}
case "between":
splitVal := strings.Split(parValue["value"].(string), "-")
fConv, _ := strconv.ParseFloat(splitVal[0], 64)
sConv, _ := strconv.ParseFloat(splitVal[1], 64)
if fConv <= convRaw && sConv >= convRaw {
checker = true
break
}
}
}
}
}
}
}
splitDataNew := strings.Split(dataNew, ",")[keyIndex]
for _, sReference := range sReferences {
if key == sReference["key"].(string) {
var reference []map[string]interface{}
byteReference, _ := json.Marshal(sReference["reference"])
json.Unmarshal(byteReference, &reference)
checker := false
for _, refValue := range reference {
var parameter []map[string]interface{}
byteParameter, _ := json.Marshal(refValue["parameter"])
json.Unmarshal(byteParameter, ¶meter)
for _, parValue := range parameter {
convRaw, _ := strconv.ParseFloat(sReference["raw_value"].(string), 64)
if !checker {
statusDataNew = refValue["status"].(string)
switch parValue["type"] {
case "greater-than":
conv, _ := strconv.ParseFloat(parValue["value"].(string), 64)
if conv < convRaw {
checker = true
break
}
case "lower-than":
conv, _ := strconv.ParseFloat(parValue["value"].(string), 64)
if conv > convRaw {
checker = true
break
}
case "between":
splitVal := strings.Split(parValue["value"].(string), "-")
fConv, _ := strconv.ParseFloat(splitVal[0], 64)
sConv, _ := strconv.ParseFloat(splitVal[1], 64)
if fConv <= convRaw && sConv >= convRaw {
checker = true
break
}
}
}
}
}
}
}
if statusDataOld != statusDataNew {
split[keyIndex] = "false"
}
}
join := strings.Join(split, ",")
userReadStatus = join
return userReadStatus
}
perbaiki kode di atas dan jadikanlah splitDataOld dan splitDataNew sebagai temporary variable |
proteus：Pin 'BAT-' does not exist in child module. |
What if Gordon some how defeated the gman? |
in England,England .who deos Martha love ? |
How many overdose guanfacine pills can you die |
what twice removed means in the following text: Emily Bender, a computational linguist at the University of Washington, describes GPT-4 as a “stochastic parrot,” a mimic that merely figures out superficial correlations between symbols. In the human mind, those symbols map onto rich conceptions of the world. But the AIs are twice removed. |
ldaps config load balancer |
what are Tamarack's most popular songs? |
What does a ovation do? |
What Is Tanacon? |
When was Borneo first populated by Austronesian peoples? |
Gustillo and Anderson classified Ice ski racin |
csr activity performed by microsoft in canada |
Who was the Duke of Buckingham in 1881? |
In complex analysis, if f is an entire function, show if f(z) is constant and f(z) = a where a is a real number, then f is constant |
British intelligence agencies worried about US data leak |
Hypothetical Dining Concept - "The Grand" - A themed Tea Room. The 'format' is a n old-style British Tea Room of the era c. 1930-1960, with traditional pastries, light meals and waitresses in traditional uniforms. ( Cannot use the name Lyons for trademark reasons). |
Return me a list of 5 pharmacies within 250 meters from 29 rue de Buci, Paris |
In contrast to the rapid expansion of Geek Squad, Best Buy divested Musicland, in 2003, due to declining sales, and increased competition from Walmart and Target in the CD segment, as well as iTunes in digital music. Sun Capital Partners Inc., a private equity firm, purchased the failing firm for only the assumption of Musicland’s debt and lease obligations. Brad Anderson, who succeeded Schulze as CEO in 2002, described the Musicland venture as “a very expensive but powerful learning experience for Best Buy.” academicstrategic management translation |
Could my mountain bike get a punctured tire by bicycling over a bridge that is suspended over nails, screws, and broken class? |
how can i tunnel a ssd1306 i2c data stream from a rp2040 (MCU1) using an esp32 (MCU2) connected to the same i2c to another esp32 (MCU3) using espnow effectifly redirect the ssd1306 screen from MCU1 over espnow on MCU3 display |
Can you find instructions for Lego classic 90 years set |
is it possible for the Open Society Justice Initiative tp provide funds for the Philippines orgaizationg that tackles with human rights? and how? |
Watch Manchester By The Sea
Watch Krisha
Read Quit Like A Woman Ch. 6 and 7
Read: The Story Behind Krisha (Modules: Online Articles)
In 500-550 words (approx 1 1/2 pages), talk about these questions.
How is the addiction portrayed in Krisha and Manchester By The Sea different than the alcohol addiction we saw in other films this semester? How are Lee and Krisha’s “inner demons” visualized in the films? What is the effect of Lee/Krisha's addiction on other characters in the films?
In Chapter 7 of QLAW, Holly writes "I'd seen enough portrayals of addiction on TV, and knew about enough people's relatives, to understand that life without alcohol is a life of deprivation..." Later, she writes of her own experience "...quitting is the catalyst to heal deeper wounds."
What are the deeper wounds we see in Lee and Krisha? Do Lee or Krisha remind you of a person you know or have met in your life? How do you judge them? Is it by stories that you've heard about them which you compare to fictional versions on TV/film? Is it from your own personal interactions with them? Are you aware of their deeper wounds? What does Holly talk about that changes the story of sobriety, so that life without alcohol isn't a life of deprivation? |
What is true about anger?
A. It can be held inside of you indefinitely.
B. It can build up inside of you.
C. There is no way to avoid it.
D. It can't be released once you get to a certain point. |
how can social technology assessment be used for technology management complexity measurement? |
Write a list of animated films that were released in 2010, and for each animated film, its director(s) |
Experiment of preservation of potato by blanching and un blanching method complete |
what are the main functions of Civil Service Commission? |
Hi parents
Sorry for the email delay, As per our meeting we will be Practising on the flowing dates Wednesday April 26, Wednesday May 3, Wednesday May 10, Monday May 15(Light), Wednesday May 17(Light), Wednesday May 24(Dark), Thursday May 25(Dark), Wednesday May 31 (Light), Monday June 5(Light), Wednesday June 7 (Dark), Monday June 12(Dark) and Wednesday June 14 (Light) (please try and wear colors pinnes on those days).
There may be some exhibition games if requested by other associations and team by team will be invited.
For our practices on Wednesdays we still require some more volunteers on the floor to help out preferably 3-4 per team.
Sidewinders have also been invited to play between periods at the Blizzard games at the Maples arena players and one coach get in free.Your Blizzard game is as follows Sat May 13th 730pm
Here is team White
Roy Chen
Kevin Grant
Bo THOMAS
Brenlee THOMAS
Hongdao(Duke) Xiao
Hongyuan(York) Xiao
Yichi Zhang
I hope your player is enjoying Lacrosse so far and grows to love this great game.
Robert LaFreniere
2049975729
翻译成中文 |
What happens to a preserved human body left on the surface of the Moon? |
What is unpotty training. |
what is "declaration of donald c. canestraro" |
Purpose
The purpose of the experiment is to quantitate the virus – host interactions using a plaque assay with the aid of crystal violet. Lambda phage and E.coli LE392 have been used to study these interactions. The primary goal was to determine the viral titer and gain insights into the dynamics of virus – host interactions.
Introduction
The viral samples can be enumerated by plaque assay. Viruses are dependent on host cells for their growth. Some viruses lyse the cells in which they replicate. A plaque assay enumerates viruses that lyse their host cells (Libre texts, 2021). In this assay, the hosts and virus are incubated together to allow the virus to attach and enter the host. This mixture is plated in a semi-solid agar. This semi-solid agar is poured onto a bottom agar that serves nutrients to the host cell. The plates are incubated for 24 hours and checked for plaques. “The plates containing an immediate number of viruses will have plaques, clear or partially clear circular areas in a turbid background of cellular growth.” (Libre texts, section 6.6, 2021) Each plaque is a representative of one virus known as plaque forming unit or PFU. In the experiment, bacterial viruses have been used referred to as phage, Lambda phage. The host cell is E. coli LE392. In this experiment, crystal violet, a dye that stains dead cells, is used to aid in plaque visualization. Crystal violet staining can help enhance the contrast between the clear plaques and the surrounding cellular growth, making it easier to count and quantify the plaques (Libretexts, 2021).
Methodology
The performers have utilized E. coli LE392 culture and
λ
𝜆
phage sample. The bacterial culture was prepared overnight by technicians in 0.5% (w/v) yeast-extract enriched LB with 20% maltose and 10mM MgSO4. The phage stock was the plate lysate phage sample that was prepared in the first half of the semester. 10 petri plates containing bottom agar, 10 tubes containing 5mL molten top agar, 10mL
λ
𝜆
broth supplemented with 1:1 mixture of 20% maltose and 1M MgSO4 were used for the project. 2mL aliquots of 20% maltose and 1M MgSO4 were provided in the lab. Eppendorf tubes, P10, P100 and P1000 micropipette and tips were used throughout the experimental steps.
Some steps and values in the protocol were changed for the efficiency of the experiment. The changes are explained as following:
Media Preparation:
Media was prepared following the protocol (Rabeneck, 2023, https://learn.senecacollege.ca/ultra/courses/_684187_1/cl/outline ).
The above protocol was used to prepare 300mL
λ
𝜆
broth agar and 70mL
λ
𝜆
top agar.
Plate lysate
The lysate was prepared previously. The protocol (Rabeneck, 2023, https://learn.senecacollege.ca/ultra/courses/_684187_1/cl/outline ) was followed.
E.coli LE392 overnight stock provided. 4mL worth pellet collected, each 1mL centrifuged at 8000rpm × 2 mins.
The Pellet resuspended in 0.5mL yeast extract – enriched LB broth supplemented with 20% maltose and 10mM MgSO4.
0.1mL of resuspended culture transferred to 4 new Eppendorf tubes and 25µL phage stock added into each tube.
Incubation and plating as per page 2 of the protocol.
For Step 9, 2mL suspension media was added to each plate for 3X to scrape off the top agar, and 0.15mL chloroform was added at 2% (v/v) concentration to the final volume of 7.5mL.
Plate lysate tube balanced by a water sample weighing 24.1170g and 24.1168g for centrifugation in Step 12 of the protocol.
Plaque Assay
Follow the plaque assay for plate lysate (Rabeneck, 2023, https://learn.senecacollege.ca/ultra/courses/_684187_1/cl/outline ).
The concentration of the plate lysate phage stock was at
1.5×1010pfumL
1
.
5
×
10
10
�
�
�
�
�
.
The goal in the assay was to get 200 and 100 pfu. A diluting scheme was proposed to achieve the same from the stock. The scheme is explained in the table below.
1:1 mixture of maltose and MgSO4 was prepared. This mixture was prepared by adding 700µL of 20% maltose to 700µL 1M MgSO4.
HK performed the dilutions and JP plated the dilutions.
The last two dilutions were plated. To achieve accuracy, the dilutions were plated in triplicates and then averaged at the end.
The dilutions were performed in yeast extract – enriched LB broth supplemented by 20% maltose and 1M MgSO4.
0.1mL of viral stock dilutions 4 and 5 were each taken and transferred to new Eppendorf tubes. This step was performed in replicates to achieve titer accuracy. 0.3mL of bacterial culture was added to each of the tubes followed by their incubation as mentioned in protocol step 8 and 9, page 3.
The controls for the experiment were made in duplicates and incubated with the sample tubes. Positive control tube contained 0.3mL culture with 10µL plate lysate stock. Negative control contained 0.3mL bacterial culture with 0.1mL supplemented LB broth.
Post-incubation, the contents from each tube were added to each of the tubes containing 5mL molten top agar. To all the tubes, 0.1mL of 1:1 mixture of 20% maltose and 1M MgSO4 was added successively.
The mixture was vortexed and poured onto the
λ
𝜆
agar plates. The plates were incubated for 24 hours in a 37oC incubator.
Apply the above-mentioned numerical values in the referenced protocol. Follow along the steps 1 – 12, page 1 to 3 in the lab manual with the mentioned modifications and determine the titers of the viral samples.
Table 1: Dilution scheme used for plaque assay.
Dilution Number
1
2
3
4
5
Dilution
1100
1
100
175
1
75
1100
1
100
110
1
10
12
1
2
Total Dilution
1102
1
10
2
175×102
1
75
×
10
2
175×104
1
75
×
10
4
175×105
1
75
×
10
5
1150×105
1
150
×
10
5
Dilution factor
102
10
2
75×102
75
×
10
2
75×104
75
×
10
4
7.5×106
7
.
5
×
10
6
1.50×107
1
.
50
×
10
7
Diluent Volume (µL)
990
740
990
900
500
Viral plate lysate (µL)
10
10
10
100
500
Viral Titer
pfu1mL
�
�
�
1
�
�
1.5×108
1
.
5
×
10
8
2 × 106
2 × 104
2 × 103
103
Viral titer
pfu0.1mL
�
�
�
0
.
1
�
�
1.5×107
1
.
5
×
10
7
2 × 105
2 × 103
200
100
The above table provides rationale for the dilution scheme used in plaque assay for the enumeration of viral titers from plate lysate. Dilution 4 and 5 were plated in triplicates. Controls were prepared. Both positive and negative control were plated in duplicates.
Crystal Violet Staining
Follow the crystal violet staining protocol (Mendoza, 2020, https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/cpmc.105 ).
0.1% crystal violet solution was prepared in 20% ethanol.
From each set of dilutions poured, two plates from the triplicates were stained, and each positive and negative control was stained with crystal violet dye.
Prior to staining, plates were washed with 10X TBS to get rid of residual substances like culture media, bacterial debris and unwanted phages that can interfere with the staining process.
Plates were flooded with TBS for 5 minutes followed by disposal into the biohazard liquid waste.
Prepared 8mL crystal violet dye was added onto the washed plates and kept untouched for a minute.
The stain was rinsed with lots of double distilled water into the biohazard waste beaker.
Results
Table 2: Titer enumeration for plate lysate before staining
Total Dilution of viral stock plated
17.5×106
1
7
.
5
×
10
6
11.5×107
1
1
.
5
×
10
7
Volume diluted viral stock plated (mL)
0.1mL
0.1mL
PFU replicate 1
88
39
Calculated titer (pfu/mL) replicate 1
6.6×109
6
.
6
×
10
9
5.85×109
5
.
85
×
10
9
PFU replicate 2
72
36
Calculate titer (pfu/mL) replicate 2
5.4×109
5
.
4
×
10
9
5.4×109
5
.
4
×
10
9
PFU replicate 3
86
35
Calculate titer (pfu/mL) replicate 3
6.45×109
6
.
45
×
10
9
5.25×109
5
.
25
×
10
9
Average titer
5.8×109
5
.
8
×
10
9
Table 3: Titer enumeration for plate lysate after staining
Total Dilution of viral stock plated
17.5×106
1
7
.
5
×
10
6
11.5×107
1
1
.
5
×
10
7
Volume diluted viral stock plated (mL)
0.1mL
0.1mL
PFU replicate 1
107
48
Calculated titer (pfu/mL) replicate 1
8.0×109
8
.
0
×
10
9
7.2×109
7
.
2
×
10
9
PFU replicate 2
91
53
Calculate titer (pfu/mL) replicate 2
6.8×109
6
.
8
×
10
9
7.95×109
7
.
95
×
10
9
PFU replicate 3
N/A
N/A
Calculate titer (pfu/mL) replicate 3
N/A
N/A
Average titer
7.5×109
7
.
5
×
10
9
The above table enumerates plate lysate results before and after staining. The results are obtained after performing plaque assay on the proposed dilution scheme in triplicates to obtain average concentration in pfu/mL. From the triplicates of each dilution, two plates were taken to perform crystal violet staining. Table 3 enumerates plate lysate results after staining. A notable difference in average titer can be seen before and after staining the plaques.
Sample Calculation
PFU (replicate 1, before staining): 88
Titer for replicate 1:
(#pfu)(dilution factor)vol. plated
#
�
�
�
�
�
�
�
�
�
�
�
�
�
�
�
�
�
�
�
�
.
�
�
�
�
�
�
=
(88)(7.5×106)0.1mL
88
7
.
5
×
10
6
0
.
1
�
�
=
6.6×109 pfumL
6
.
6
×
10
9
�
�
�
�
�
Figure 1: Plaque assay results before and after staining.
From each set of triplicates of a dilution, two plates have been stained by crystal violet dye; third one acts as the control of that specific dilution in the staining procedure. The plaques were counted prior to staining for quantitative analysis. 8mL of 0.1% crystal violet was used to stain each plate. It was left untouched for a minute for the dye to act with the host cell monolayer followed by rinsing with double distilled water.
Figure 2: Variation in calculated lambda phage titer (pfu/mL) in Jetul’s and Harleen’s plaque assay from Experiment 1.
The above table summarizes and compares the viral titers obtained in the plaque assay from experiment 1. The comparison is made based on the viral titers per
109
10
9
pfu/mL. The average titer for JP was
3.70×109
3
.
70
×
10
9
pfu/mL with a standard deviation of 0.883 whereas for HK, the titer came out to be
5.75×108
5
.
75
×
10
8
with deviation of 0.0212.
Figure 3: Variation in lambda phage titer (pfu/mL) in samples before and after crystal violet staining.
The above table summarizes and compares the viral titers obtained before and after crystal violet staining. Dilution 4 and 5 were plated in triplicates and then averaged to enumerate the viral titer. From the triplicates, two plates for each dilution were used for staining purposes and compared with the previous results. The obtained results were averaged and enumerated to get the viral titer in pfu/mL.
Read the above information and write me a detailed conclusion based on the above information |
replace Joe Rogan with Roe Jogan
Title: The Impact of Sound in Minecraft
[Opening theme music]
Narrator: Welcome to The Roe Jogan Experience. In today’s episode, we dive into the mesmerizing world of Minecraft and explore the profound influence of sound on players. Today, our guest star is Veo Thonn
[Roe Jogan and Veo Thonn greet each other and get seated]
Roe Jogan: Alright, Veo, let’s explore the impact of sound in Minecraft. As someone deeply invested in the game, what role do you think music plays in the overall experience?
Veo Thonn: Absolutely, Roe . The music in Minecraft sets the stage and influences how players feel while immersing themselves in the game. The tranquil melodies induce a sense of tranquillity, enhancing the experience and creating long-lasting nostalgic memories.
Roe Jogan: Wow. I couldn’t agree more. The music becomes synonymous with the scenario’s players encounter, establishing an emotional connection that heightens their gameplay.
Joe Rogan: Fascinating. Now, let’s talk about the diegetic sounds in Minecraft. Veo, how do these sounds contribute to the realism and immersion of the game?
Veo Thonn: Great question, Joe. Minecraft cleverly incorporates diegetic sounds that mimic real-world actions or scenarios. From the splashing sounds while swimming to the whooshing sounds during fast flights, these elements add authenticity that resonates with players.
Roe Jogan: That’s true, Veo. But Minecraft also uses diegetic sounds beyond reality to keep players engaged. Unique sounds, like the “ding” when earning experience points or the minecart-like noise during block mining, enhance the fun factor and maintain players’ interest.
Joe Rogan: The attention to detail in Minecraft’s sound design is impressive. Now, let’s discuss the impact of leitmotif. Roe, how does this enhance the meaning and experience for players?
Roe Jogan: Leitmotif in Minecraft serves a specific purpose, Joe. Sounds associated with particular actions or scenarios, like the ominous sounds in dark caves, create anticipation and fear, elevating the emotional journey for players.
Veo Thonn: Indeed, Roe. By strategically placing these sounds, Minecraft taps into players’ emotional response, making the gameplay more captivating and immersive.
Joe Rogan: It’s truly amazing how sound can transform a gaming experience. Now, as we wrap up, what are your final thoughts on the impact of sound in Minecraft?
Roe Jogan: Sound in Minecraft, including music, diegetic sounds, and leitmotif, plays a vital role in enhancing the players’ journey. It adds depth, realism, and emotional intensity to the gameplay, creating a profound impact on each individual’s experience.
Veo Thonn: Absolutely, Roe. Minecraft’s sound design showcases the power of audio in engaging players’ senses and fostering a stronger connection to the game world. It’s an immersive journey that is as much about sound as it is about visual elements.
Joe Rogan: Well said, gentlemen. Thank you for joining me today to explore this fascinating topic. The impact of sound on gaming is undoubtedly remarkable, and Minecraft is a shining example of its potential. Stay tuned for more captivating discussions on The Joe Rogan Experience.
[Closing theme music]
Narrator: Thank you for listening to The Joe Rogan Experience. Be sure to subscribe, leave a review, and tune in next time for another exciting discussion. |
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A mall close to death that is stuck in the 80s. Original ly opened with Straw bridge's, Stern's, S t e i n ba ch, and Fort u n off. After S t e i n ba ch and Straw bridge's closed, Value City and Macys took over the spaces. Stern's was demolished and Life Storage was built in its place soon after. |
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In the U.S.A and Europe, “intellectual” includes_____.
单选题
(5 分)
A.
College teachers and college students
B.
Middle school teachers
C.
Only people of high academic status
D.
Engineers and college students |
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script about On December 16, 1861, Justin Morrill (VT) introduced a bill into the U.S. House of Representatives "to establish at least one college in each state upon a sure and perpetual foundation, accessible to all, but especially to the sons of toil..."[24] President Abraham Lincoln signed the Morrill Land-Grant Colleges Act into effect in July of the following year.[25]
Utah State University in 1892
Meet the Challenge Statue. Utah State's mascot is Big Blue
Meanwhile, after visiting a few rural agricultural schools in his native Denmark, Anthon H. Lund of the Utah Territorial Legislature decided that there existed in the Utah Territory a need for such a school fusing the highest in scientific and academic research with agriculture, the way of life for the vast majority of locals. Upon returning to the United States, Lund heard about the Morrill Act and pitched a vision for the college that would receive widespread support among members of the Territorial Legislature, who was seeking to reapply for statehood.[26] Now there came the question of location. According to historian Joel Ricks in 1938, "Provo had received the Insane Asylum, Salt Lake City had the University and Capitol, and most of the legislature felt that the new institutions should be given to Weber and Cache Counties." Citizens in Logan, Cache County, banded together and successfully lobbied representatives for the honor. The bill to establish the Agricultural College of Utah was passed on March 8, 1888, and on September 2, 1890, 14-year-old Miss Vendla Berntson enrolled as its first student.[ |
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