Will be supplied as "ActiTaq delta exo Genotyping Master". Unit of measure is "mL".
Hot start DNA master mix containing a 5'-3' exonuclease deficient hot start Taq DNA polymerase for optimal detection of mismatches.
The 5x concentrated master mix contains a chemically modified truncated Taq DNA Polymerase and all reagents (except primers, probes, and template) needed for real-time DNA detection assays with various probe formats except for hydrolysis probes due to the lack of 5'-3' exonuclease activity.
Discriminate paired and unpaired primer ends using an enzyme optimized for allele-specific PCR.
Pipett and handle the hot start reaction mix at ambient temperature.
Create capable PCR assays with a minimum of optimization efforts, also multiplex PCR applications.
The master mix can be stored in the refrigerator (+2 to +8°C) for at least 4 week without loss of activity and performance. The complete PCR mix (Master + primers + probes + template) is stable for up to 24 hours at +15 to +25°C.