Post autoclave the media was allowed to cool and just above setting point 6ml/L 3% H2O2 was added.
Thanks Darklight - I used the peroxide agar method for years with good success - very few contaminations.
My method is essentially the same of yours but with only 7ml/L of 3% H2O2 into autoclaved media (all kinds of different media used) also the H2O2 was added when the temperature of the media was just below 60 degrees.
*optionally (and often) I would add a pinch of any range of flours, wheat rice, corn etc. I also liked to add powdered straw/ wood/ manure depending species I was growing.
I actually promised Worowa that I would reinstate his fungal collection since he moved - so I will be doing this all again soon!
Edit: I was going to say 60ml/L H2O2 seams like too much peroxide to me. (?) & apparently some species don't like H2O2. although I have yet to find one though.
Hope no-one copied and pasted it, thanks for bringing it to my attention so fast!
I too have used the H2O2 method with great success. certainly thumbs up from me.
i think mine might be a little stronger with between 8 ml/L to 10 ml/L 3% H2O2, but i dont notice any problems.
I use the hydroponic h2o2 and it has colloidal silver in it . I use it in a lot and it works wonders .
Thanks for the tek, its great for newbies and will give it a try next batch.
gee thanks so much - can i put this onto a document?
I've used from 3mL/L to 25mL/L...always works, except for a "stropharia rugoso-annulata" (never got it to fruit, so not sure if it was the real deal). Mycelium moves slower with peroxide, but that's a good thing usually. 1000's of plates, no contams. Still get the occasional grain spawn contam, but I've never used peroxide in grain.
My current recipe, which probably needs updating to avoid Nestle is 1 tablespoon malted milo, 1 tablespoon organic black strap mollasses, 1 cup of freshly brewed black coffee, 25 grams agar (1 packet swan brand), top up with boiled tap water to 2L. The extra agar makes it set thick, which makes it easier to take wedges out. This is nice and dark, easy to see mycelium. Add peroxide between 50 and 60 degrees.
a 90mm plate. yep peroxide does slow the growth.
the next transfer on to a non-h2o2 and this thing unleashed itself.
this is a 90mm plate, 36hrs after the transfer.
actually is scares me, I don't know what I have captured.
I had a 110% success rate adding 2ml during the open air boil (in a flask tho) then 3ml (both instances 6%) right before pouring.