Guiseley's relegation from the Vanarama National League was confirmed with a 1-0 home defeat to Barrow.
The Lions have won only six league games all season and Asa Hall's second-half goal condemned them to the drop.
The away side dominated from the off as Joe Green saved Lewis Walters' effort from the edge of the box.
Green then brilliantly tipped Dan Jones' shot around the post before Luke James headed wide from inside the six-yard area.
Dean Rance's first-half goal proved decisive as Ebbsfleet triumphed 1-0 at relegation-threatened Barrow in the National League.
Rance lashed home on 32 minutes, following up after Sean Shields had rattled the bar.
The visitors hit the post twice but were unable to add any further goals.
The struggling hosts found chances hard to come by, even with seven minutes of stoppage time due to an injury to a linesman, and remained deep in trouble in 21st spot.