NOTES: Mix media & Dispase II, then filter sterilize (40 uM). Do not add Dnase I until just before use.
If not sorting Make aliquots of 400-500,000 cells in FACS tubes for unstained/control samples and 200,000 cells for stained samples, spin down, resuspend each in 200 uL of PBS + 2% CS.
If sorting: From sample with largest cell #, make aliquotes of 200k cells for unstained/control samples, spin down, resuspend in 200 uL of PBS + 2% CS. Leave samples that are to be sorted in original volume in 15-mL tubes when applying stains.