You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Structure of proteins can be detected by all the methods, EXCEPT: and explain in detail?
High-performance/pressure liquid chromatography (HPLC) is a technique used for enhanced and precise separation of even small amounts of biological and nonbiological molecules. HPLC columns utilize variations of the separation principle behind column chromatography, depending on variable attractive and repulsive forces on the gel material and their interaction with the biological molecules. The analysis of peptides and structure of proteins are done by mass spectrometry. NMR spectroscopy analyzes proteins in aqueous solution. Proteins and protein complexes of >100 kDa can be analyzed by NMR. Ref: Janson L.W., Tischler M.E. (2012). Appendix II. Biochemical Methods. In L.W. Janson, M.E. Tischler (Eds), The Big Picture: Medical Biochemistry.