You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Which of the following is the best method for monitoring thiamine level in blood? and explain in detail?
Thiamine deficiency is diagnosed by an increase in erythrocyte transketolase activity observed upon addition of thiamine pyrophosphate. The activation of apo-transketolase (the enzyme protein) in erythrocyte lysate by thiamine diphosphate added in vitro has become the accepted index of thiamine nutritional status. Ref: Harper's Illustrated Biochemistry, 26th Edition, Pages 488-489 ; Lippincott Biochemistry, 2nd Edition, Page 322 ; Textbook of Biochemistry By D M Vasudevan, 3rd Edition, Page 269