You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Number of isoenzymes in LDH enzyme - and explain in detail?
(D) (5) (65 Harper 29th) * LDH has 5 isoenzymes while CPK has three. * LDH is an oligomeric (tetrameric) enzyme made up of four polypeptide. * Two types of subunits namely M (for muscle) is basic and H (for heart) is acidic. * LDHi has more positive charge and fastest in electrophoretic mobility while LDHS is slowest. * LDH5 has low Km (high affinity) while LDH1 has high Km (low affinity) for pyruvate. * Normal CSF LDH is 5 to 40 U/L. Similar to AST (Aspartate transaminase). Increase of CSF LDH observed in abscess, cerebral haemorrhage / infarction and metastatic carcinoma. * Increase of LDH4 isoenzyme of CSF has been reported in tubercular meningitis. * Normal serum LDH activity ranges from 60 to 250 IU/L (120 - 500 units / mL) Behavior in Acute MI In acute MI, serum activity rises within 12 to 24 hours attains peak at 48 hours (2 to 4 days) reaching about 1000 IU/L and then return gradually to normal from 8th to 14th day. * The predominant isoenzyme of LDH in cardiac muscle is LDH-1 (in Liver - LDH-5) * Albumin helps in distinguishing an acute liver disease from chronic liver disease.