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What is Acridine orange is a fluorescent dye used to bind? and explain in detail?
Ans. (a) DNA and RNARef: Harrison /150e-2* Acridine orange is a nucleic acid selective-fluorescent cationic dye useful for cell cycle determination.* It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions respectively and emits green and red right respectively.* Acridine orange can be used in conjunction with ethidium bromide to differentiate between live and apoptotic cells.