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What is Fluoride released from fluoroacetate inhibits which metabolic pathway? and explain in detail?
Fluoroacetate is toxic because fluoroacetyl co A condenses with oxaloacetate to form fluorocitrate,which inhibits aconitase,causing citrate to accumulate. Other inhibitors of citric acid cycle-Malonate,Arsenite. Enolase requires Mg++, and by removing magnesium ions,fluoride will reversibly inhibits this enzyme.Thus,fluoride will stop the glycolysis. So when blood for sugar estimation,fluoride is added to blood.If not,glucose is metabolized by the blood cells,so that lower blood glucose values are obtained. Reference: Harpers illustrated biochemistry,30th edition