You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Thiamine deficiency causes lactic acidosis due to defect in the action of? and explain in detail?
Ans. d (Pyruvate dehydrogenase). (Ref. Harper, Biochemistry, 25th ed., 197)Inhibition of pyruvate metabolism leads to lactic acidosis.# Arsenite, mercuric ions, dietary deficiency of thiamine, inhibits pyruvate dehydrogenase, and allows pyruvate to accumulate.# Thiamine is a cofactor for several enzymes like- Transketolase,- Pyruvate dehydrogenase and- a-KG dehydrogenase.# If nutritionally deprived alcoholics are given glucose, thiamine deficiency causes rapid accumulation of pyruvate and lactic acidosis. Patient with inherited pyruvate dehydrogenase deficiency have same features particularly after glucose load.#IV thiamine is treatment of choice in Wernicke's encephalopathy.QINHIBITORS OF METABOLIC PATHWAYS EnzymeInhibitorPathway1.AconitaseFluoroacetateKreb's cycle2.L-KG dehydrogenaseArseniteKreb's cycle3.EnolaseFluorideGlycolysis4.Succinate dehydrogenaseMalonate oxaloacetateKreb's cycle5,Giyceradehyde-3 phosphatelodoacetate dehydrogenaseGlycolysis6.MOAIproniazid 7.Pyruvate dehydrogenaseArsenite 8.Carbonic anhydraseSulfanilamide