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What is Klenow fragment lacks the activity of? and explain in detail?
Ans: B. 5'-3' exonucleaseDNA polymerase I is not the primary enzyme of replication; instead, it performs a host of clean-up functions during replication, recombination, and repair.When the 5'-3' exonuclease domain is removed, the remaining fragment (Mr 68,000), the large fragment or Klenow fragment, retains the polymerization and proofreading activities.Klenow fragment is a large protein fragment produced when DNA polymerase I from E. coli is enzymatically cleaved by the protease subtilisin.