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What is Protein is purified using ammonium sulfate by and explain in detail?
The process of protein precipitation by the addition of neutral salts such as Ammonium sulfate or Sodium sulfate is called salting out. This phenomenon is explained on the basis of dehydration of protein molecules by salts. This increased protein_protein interaction,resulting in molecular aggregation and precipitation.TSalting out procedure is conveniently used for separating serum albumins from globulins