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What is Effective mode of sterilization is: September 2005 and explain in detail?
Ans. B: Steam under pressure Boiling and steam at atmospheric pressure are ineffective means of killing bacterial spores. Boiling requires prolonged period of boiling for killing spores whereas steam at atmospheric pressure may fail with spores of ceain anaerobes and thermophiles. Nothing sho of autoclaving at high pressure can destroy spores and ensure sterilization.