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What is Southern blot is used for and explain in detail?
SOUTHERN BLOT : It is based on the specific base pairing propeies of complementary nucleic acid strands ,this technique is based on DNA hybridization. DNA is isolated from the tissue.It is then fragmented by restiction endonucleases.The cut pieces are electrophoresed on agarose gel. Treatment with NaOH denatures the DNA, so that the pieces become single stranded. This is then blotted over to a nitrocellulose membrane .The ssDNA is adsorbed on the nitrocellulose membrane. An exact replica of the pattern in the gel is reproduced on the membrane. REF :DM VASUDEVAN TEXTBOOK; 8th edition;Page no :609