You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Tone of LES is maintained by and explain in detail?
Acetylcholine and tachykinins are involved in the LOS contraction acting through acetylcholine M3 and tachykinin NK2 receptors. Nitric oxide can also be involved in the regulation of LOS contraction. Ref: guyton and hall textbook of medical physiology 12 edition page number:443,444,445