You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Biuret test is used for detection of: and explain in detail?
Ans: a (Protein) Ref: Vasudevan 4th ed/p.23, 24. 34.Biuret reaction:-* Cupric ions chelate with peptide bonds of proteins in alkaline medium to produce a pink or violet colour. This needs a minimum of two peptide bonds and so individual amino acids and dipeptides will not answer this test.* Biuret reagent - CuS04, sodium potassium tartarate and potassiumiodide in sodium hydroxide.* The intensity of colour is directly proportional to the peptide bonds and is estimated colourimetrically.Other tests:Fouchet'stest-Bile pigmentsHay's sulphur test- Bile saltsBenedict's test- Blood sugarRothera's test- Ketone bodiesReagentsEhrlich's aldehyde - Porphobilinogen and UrobilinogenFouchet's reagent - Bile pigmentBenedict's reagent - GlucoseSodium nitroprusside - Ketones