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What is Robert Koch assistant advised him to use agar instead of gelatine for solidifying culture media for cultivation of bacteria as - and explain in detail?
Agar is preferred to gelatin as a solidifying agent in culture media.
This is because gelatin melts at relatively low temperature (<35o C), while agar melts at around 85° C.
Therefore, when gelatin is incubated at the normal temperature for bacterial growth, i e. at 37° C, it melts, while agar remains solid.