You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is During DNA replication, which of the following enzymes produces a sho strand of RNA complementary to the template DNA with a free 3'-OH end? and explain in detail?
The primer molecule required by DNA polymerase is a sho strand of RNA (4-10 bases) complementary to the template strand of the DNA molecule. The primer is synthesized by a specific RNA polymerase known as primase. The growing end of the RNA primer is a free 3'-OH group. The primase does not itself require a primer for initiation of nucleotide synthesis. DNA ligase catalyzes the formation of a phosphodiester bond between the 3'-OH of one fragment of DNA and the 5'-monophosphate group of an adjacent DNA fragment. Polymerase I catalyzes the polymerization of nucleotides and also functions in processing and repair mechanisms. Polymerase III is a pa of a multiprotein complex and is the major replicating enzyme in E. coli.