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What is True about Hybridoma: and explain in detail?
A i.e. Immoalise myeloma cell - Hybridoma is an immoal (permanent) cellQ produced by cell fusion of B cells (previously immunized or injected with antigen) and myeloma cells of mouseQ (or suitable animal). It produces monospecific monoclonal antibodiesQ which can be humanized (used in humans) by attaching the complementarity - determined regions , (the site that binds antigens) onto appropriate sites in human immunoglobulin molecule. Attempts to produce human monoclonal antibodies are underway. - Monoclonal antibodies are produced by hybridoma technology (in vitro) or by one clone secreting specific type of antibody in multiple myeloma (in vivo). It is required in minute (only microliter) quantities and can be used for blood groupingQ.