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What is Restriction endonuclease cleaves and explain in detail?
Restriction endonuclease Cleaves double stranded DNA at palindromic sites/ restriction sites 5' --AAGCTT-- 3'3' --TTCGAA-- 5' A palindromic sequence is a sequence which reads the same backwards as forwards. Found in bacteria Can create sticky ends or blunt ends Restriction endonuclease Sticky ends Blunt ends single-stranded overhangs no overhangs Example - EcoRI Example -SmaI