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What is PCR requires and explain in detail?
A primer is a sho strand of RNA or DNA (generally about 18-22 bases) that serves as a staing point for DNA synthesisPolymerase chain reaction uses DNA primers because they are more temperature stable. In experiments, it is often impoant to use a primer with a similar Tm to the template strand it will be hybridizing to.Ref: DM Vasudevan, 7th edition, page no: 638