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What is Vitamin C is necessary in the formation of collagen. It is required for the conversion of: and explain in detail?
Ans. (a) Proline to hydroxyprolineRef: Harpers Biochemistry, 30th ed. pg. 47, 562* Ascorbic acid is needed for a variety of biosynthetic pathways, by accelerating hydroxylation and amidation reactions.* In the synthesis of collagen, ascorbic acid is required as a cofactor for following enzymes:# Prolyl hydroxylase# Lysyl hydroxylase* These two enzymes are responsible for the hydroxylation of the proline and lysine amino acids in collagen.* Remember, Hydroxyproline and hydroxylysine are important for stabilizing collagen by cross-linking the propeptides in collagen.* Clinical effects if there is defective collagen fibrillogenesis:# Impaired wound healing.# Impaired bone bormation# Abnormal bleeding due to fragile capillaries.