You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Radio mi metric BACTEC detect growth of M tuberculosis in how much time - and explain in detail?
Ans. is 'b1 i.e., 2-3 weeks Laboratory diagnosis of T.B. T.B. Specimens Decontamination and concentration of specimens Smear Culture Nucleic acid amplification Immunodiagnosis o Sputum o Urine o Pleural fluid o CSF o Joint fluid o As sputum and other specimens frequently contain many bacteria & fungi that would rapidly overgrow mycobacteria on culture, they should be destroyed o Methods y Petroff's method (M.C.) y Flotation method y Autoclave method y Treatment with antiformin, Na2CO3 or hypochlorite y Detergents like tergitol o Ziehl-Neelsen staining o Fluorescence microscopy using auramine-rhodamine stain (more sensitive & rapid) Solid medium o L.J. medium (4-8 wk for growth) Liquid media o BACTEC-460 | Radiomimetic growth detection Within 2-3 wks. o PCR o Ligase chain reaction o R.N.A. specific PCR o Not very useful o Enzyme immune assay for detection of antibody to mycobacterial lipoarabinomannan o Definitive diagnosis depends on isolation and identification of M. tuberculosis from a diagnostic specimen. Remember y Sputum is best collected in the morning before any meal. y Sputum sampling on three days increases the chances of detection. y If sputum is not produced, bronchial washing, brushing or biopsies and early morning gastric aspirate may be examined. y At least 10000 (HP) bacilli should be present per ml of sputum for the demonstration in a direct smear. y Culture can detect 10-100 bacilli per ml. o For culture o For microscopy - - 10-100 bacilli per ml. > 10000 (104) bacilli perml.