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What is The laboratory diagnosis of thiamine deficiency is by: and explain in detail?
The laboratory diagnosis of thiamine deficiency usually is made by a functional enzymatic assay of transketolase activity measured before and after the addition of thiamine pyrophosphate. >25% stimulation by the addition of thiamine pyrophosphate is interpreted as abnormal. Thiamine or the phosphorylated esters of thiamine in serum or blood also can be measured by high-performance liquid chromatography to detect deficiency.