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What is Enzyme specificity is given by - and explain in detail?
Ans. is "a' i.e., Km "The of an enzyme is the concentration of the substrate that enables the enzyme to function at half maximum activity and is therefore a measure of the specificity of a substrate for the enzyme"___________Clinical biochemistryo Actually enzyme specificity is not measured by Km alone.o It is measured by the ratio Kcat/Km which is a second order rate constant for the reaction between substrate and free enzyme.o This ratio is important, for it provides a direct measure of enzyme efficiency and specificity,o Note : Kcat is turnover number and measures the rate of the catalytic process.