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What is Chimeric DNA used for: and explain in detail?
Ans. (d) Organ transplantationRef: Harpers Biochemistry 30th Ed; Page No- 459* Chimeric DNA is known as Recombinant DNA.* Chimeric DNA = DNA to be cloned + Vector DNAMote* Passenger DNA: DNA to be cloned is called Passenger DNA or Foreign DNA.* Procedure to Prepare Chimeric DNA:# Both foreign DNA and Vector DNA is treated with same restriction endonuclease.# This creates sticky or blunt ends.# This is religated using DNA ligase.# Thus chimeric DNA os produced.* Organ transplant will be right answers: for synth of GSM -CSF for stimulating bone marrow after marrow transplants.* So expression vector or chimeric DNA or recombinant DNA technology!* Uses of Recombinant DNA technology:# Predict the risk of genetic defects.# Preparation of Vaccine and Hormones.# Diagnosis of infectious disease.# Gene therapy# Forensic medicine: Reveal a criminal from specimens left on the scene of crime.