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What is Which of the following are AFB positive with 5% sulphuric acid - and explain in detail?
Procedure : 1. The smear is stained by a strong solution of carbon fuchsin with the application of heat 2. It is then decolorized with 20% SULPHURIC ACID and counterstained with a contrasting dye such as methylene blue 3. The acid-fast bacteria retain the fuchsin color while others take the counterstain Mycobacterium leprae resists decolorization with 5% SULPHURIC ACID Ref: Ananthanarayan & paniker's Textbook of Microbiology 9th edition pg no 14