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What is Which of the following is not required for protein synthesis of eukaryotes: and explain in detail?
Ans. a. RNA polymerase (Ref Harper 28/e p362) RNA polymerase enzyme is involved in transcription process, not in translation.The a-amino group of the new aminoacyl-tRNA in the A site carries out a nucleophilic attach on the esterified carboxyl group of the peptidyl-tRNA occupying the P site (peptidyl or polypeptide site).Peptidyl transferase: Catalyses two reactions, peptide bond formation between amino acids and together with release factor, peptide release.