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What is Single base pair substitution is related to which of the following? and explain in detail?
The term RFLP (Restriction Fragment Length Polymorphisms) refers to single base pair substitutions in germ line DNA of different individuals that either destroys or creates new recognition site for a given restriction enzyme. PCR is used to amplify a specific region of a DNA strand (the DNA target). FISH is atechnique used to identify the presence of specific chromosomes or chromosomal regions through hybridization (attachment) of fluorescently-labeled DNA probes to denatured chromosomal DNA. Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.