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What is Which of the following procedures is used as a routine technique for karyotyping using light microscopy? and explain in detail?
Giemsa stain is the most commonly used staining method that allow identification of each individual chromosome, on the basis of a distinctive and reliable pattern of alternate light and dark bands along the length of the chromosome. Since giemsa stain is used, the procedure is called G banding. Ref: Robbins Pathologic Basis of Disease, 6th Edition, Pages 165-6