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What is What is the staining technique used here and explain in detail?
Acid-fast Pink bacilli in the blue background; M.tuberculosis Most isolates of Mycobacterium, Nocardia, and Rhodococcus species are acid-fast. Acid-fast bacteria are those that retain carbolfuchsin (basic fuchsin dissolved in a phenol-alcohol-water mixture) even when decolourized with hydrochloric acid in alcohol (due to mycolic acid in cell wall) Ref : Ananthanarayana textbook of Microbiology 9th edition Pgno : 13-14