You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is In a reaction substrate is available in a concentration that is l000times the Km value of the enzyme. After 9 minutes of reaction, l7o substrate is conveed to product (12 microgram/ml). If the concentration of the enzyme is changed to 1/3 and concentration of substrate is doubled. What is the time taken to conve the substrate into the same amount of product, i.e. I 2microgram/ml? and explain in detail?
Ans: C. 27 minutesRef: Lehninger principles of biochemistry, 6t' ed., pg. 204During derivation of Michaelis-Menten equation,If total enzyme concentration is reduced by 1/3 and is very high then Vmax also become l/3.So in this question same amount of product will be fbrmed in 27 rninutes (3 x 9min)