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What is All the following can be used to detect the mutation, except and explain in detail?
Methods used for detecting mutations 

Cytogenetics analysis  
FISH   
PCR, 
RFLP 
DNA sequencing, Southern blotting 
Microarrays 
Single-stranded conformational polymorphism (SSCP)
RNAase cleavage
Denaturating gradient gel electrophoresis (DGGE)
Protein truncation test