You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Nohern blot test is used for? and explain in detail?
The nohern blot is used to demonstrate specific RNA. The total RNA is isolated from the cell, electrophoresed and then blotted to a membrane. This is then probed with radioactive cDNA. There will be RNA-DNA hybridization, if the specific RNA is present in the cell. For DNA analysis southern blot technique is used, western blot analysis is used to identify proteins.Also Know:A fouh hybridization technique, the Southwesternblot, examines protein-DNA interactions. In this method, proteins are separated by electrophoresis, blotted to a membrane, renatured, and analyzed for an interaction with a paicular sequence by incubation with a specific labeled nucleic acid probe. Ref: Weil P. (2011). Chapter 39. Molecular Genetics, Recombinant DNA, & Genomic Technology. In D.A. Bender, K.M. Botham, P.A. Weil, P.J. Kennelly, R.K. Murray, V.W. Rodwell (Eds), Harper's Illustrated Biochemistry, 29e.