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What is Enzyme catalyzing reversible step in glycolysis is/are and explain in detail?
In step 8 of glycolysis i.e 2- phosphoglycerate is conveed to phosphoenol pyruvate by the enzyme enolase.One water moleculeis removed. A high energy phosphate bond is produced.The reaction is reversible. Enolase requires Mg ++ , and by removing magnesium ions, fluoride will irreversibly inhibit this enzyme.Thus, fluoride will stop tge whole glycolysis.So when collecting blood for sugar estimation, fluoride is added to blood.If not , glucose is metabolised by the blood cells, so that lower blood sugar values are obtained. REFERENCE: DM.VASUDEVAN.TEXTBOOK SEVENTH EDITION ; page no: 111