You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is A 16-year-old boy had a flea bite while working in a wheat grain godown. After 4 days he developed fever and had axillary lymphadenopathy. The stain used for smear preparation is and explain in detail?
This is a case of plague--Working in godown (rats commonly found) H/o flea bite, In patient 5 days, fever and lymphadenopathy. Wayson stain a polychromatic stain- Y. Pestis can be best demonstrated (other is Giemsa) Bipolar staining or safety pin appearance is seen. Albe stain use for C. diphtheria Z-N stain use for M. tuberculosis and other acid-fast bacteria Mc Fayden reaction used for B. anthracis Ref : Ananthanarayana textbook of Microbiology 9th edition Pgno : 320