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What is Light microscope resolution is? and explain in detail?
The resolving power of the light microscope is limited by the wavelength of light. In order to be seen and delineated (resolved), an object has to have a size of approximately half the wavelength of the light used with visible light using the best optical systems, the limit of resolution is about 300nm. If the light of sho wavelength is employed, as in the UV microscope the resolving power can be propoionately extended. Ref: Textbook of Microbiology; Ananthanarayan and Paniker; 9th edition; Pg no: 10