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What is Mucin is stained with: and explain in detail?
Ans: b (Alcian blue) Ref: Harsh mohan, 4th ed,p. 26Mucin is a glycoprotein produced by the epithelial cells of mucous membrane and mucous glands as well as by some connective tissue like in the umbilical cord.Stains:Epithelial mucin stains positive with- PASConnective tissue mucin with- Colloidal ironBut both are stained by- Alcian blue onlyApart from mucin, alcian blue stains many other structures- ocular basement membrane capsule, mast cell, proteoglycanStructures and stains:Fat- oil red O, osmium tetroxide, sudan blackCalcium- von kossa, alizarin redConnective tissue- von geisson, masson trichromeMelanoma-masson FontanaCarbohydrate-PASGlycoprotein, glycolipid, proteoglycan}PAS with diastase, alcian blue, mucicarmine, bestcarmineInsulin- gomori aldehydeSideroblast-Prussian blueDNA- fuelgen, acridine orange, silver stain Amniotic fluid cells- nile blue sulphateHeinz bodies, reticulocytes - supravital stains