You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Triple test for diagnosis of Down's syndrome includes all of the following except – and explain in detail?
Triple test is a combined biochemical test which includes → MSAFP, HCG, & Oestriol

In an affected pregnancy  
level of MSAFP is decreased
level of oestriol is decreased
the level of hCG is High (increased)