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What is Which organism cannot be cultured in cell free media -a) Klebsiella rhinoscleromatisb) Klebsiella ozaenaec) Treponema pallidumd) Pnemocystis jirovecie) Rhinosporidium seeberi and explain in detail?
Treponema pallidum is an abligate intracellular organism. Thus it cannot be cultivated in cell free medium.
Pneumocystis Jiroveci and Rhinosporidium seeberi can not be cultivated.