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What is Best fixative for Pap smear: and explain in detail?
ANS. AFixatives:* Pap smear: 95% ethanol + 5% ether* Light microscopy: 10% formaldehyde* Electric microscopy: 2% glutaraldehyde* For liquid-based cytology: Methanol* For bone marrow biopsy: Zenker's fluid* For bone marrow aspirate: Helly's fluid* Cell block: Bouin's fluid* Karyotyping fixatives: Carnoy's fixative (3:1 methanol to glacial acetic acid)* Gastrointestinal biopsies: Bouin's fluid.