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What is Antigen-antibody reaction is detected by (PGI Nov 2009) and explain in detail?
Ans: A (ELISA) & D (Western blot) "In western blots (also called immunoblots) protein molecules in the sample are separated by electrophoresis & blotted (transferred) to a membrane. The probe is a labeled antibody, which produces a band at the location of its antigen"- Lippincott's Biochemistry 4th/484Table (Lippincott's Biochemistry): Techniques used to analyze DNA, RNA & proteinsTechniqueSampleAnalysedGelUsedPurposeSouthern blotDNAQYesDetect DNA changesNorthern blotRNAYesMeasures mRNA amounts & sizeWestern blotproteinYesMeasures protein amountsASODNAQNoDetects DNA mutations''MicroarrayRNA or cDNANoMeasures many mRNA levels at onceELISAproteins or antibodiesNoDetects proteins (anti- genes or antibodies^ProteomicsProteinsQYesMeasures abundance, distribution, post-translational modifications, functions & interactions of cellular proteinsASO= Allele-specific oligonucleotides