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What is Not used in grams staining? and explain in detail?
GRAM STAINING 1)primary staining with a pararosaniline dye such as crystal violet, methyl violet, or gentian violet 2)application of a dilute solution of iodine 3) decolorization with an inorganic solvent such as ethanol, acetone, or aniline 4)counterstaining with a dye of contrasting color such as carbon fuchsin, safranine or neutral red REF:Ananthanarayan & Panicker's Textbook of Microbiology 8th Edition pg no: 15