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What is Which organism cannot be cultured in cell-free media and explain in detail?
Treponema cannot be grown in aificial culture media but is maintained by subculture in susceptible animals. Sporothrix is a dimorphic fungus, that form yeast in tissues at 37C and the mycelial phase in the culture at 25C. Klebsiella grows on ordinary media. Reference: Textbook of Microbiology; Ananthanarayan and paniker's; 10th edition; Pg: 379