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What is The function of Restriction II enzyme after binding to a unique recognition site is which of the following? and explain in detail?
Type II restriction endonucleases recognize and bind specifically to a unique recognition site that is typically a 4-, 6-, or 8-bp palindrome. After binding they introduce a double-stranded cut at the sequence. Restriction cleavage occurs at one site producing "blunt ends" or, if it occurs in a staggered fashion, "overhanging ends". Ref: DNA Structure and Function By Richard R. Sinden, 1994, Page 55; Human Molecular Biology: An Introduction to the Molecular Basis of Health ... By Richard J. Epstein, 2003, Page 536