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What is The dye in Fluorescent microscopy? and explain in detail?
Auramine Rhodamine is the Dye for Fluorescent microscopy. Smears are stained with Auramine phenol or Auramine Rhodamine fluorescent dyes and when examined under UV illumination the bacilli will appear as bright rods against a dark background. Ref: Textbook of Microbiology; Ananthanarayan and Paniker; 9th edition; Pg no: 353