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What is Kelly's medium is used in the isolation of ? and explain in detail?
Ans. is 'b' i.e., Borrelia . Modified Kelley's (BSK) medium is used for Borrelia burgdorferi. Laboratory diagnosis of Lyme disease . The culture of B. burgdorferi in Barbour - Stoenner - kelly (BSK) medium permits definitive diagnosis, but this complex method has been used only research studies. . Because of the problems associated with direct detection of B. burgdorferi, lyme disease is usually diagnosed by the recognition of a characteristic clinical picture with serological confirmation. . A two step approch is used in which samples are first tested by ELISA and equivocal or positive results are then tested by western blotting. . Major use for PCR testing in lyme disease --> Detection of B. burgdorferi in synol fluid.