You are a helpful, respectful, and honest assistant. Always answer as helpfully as possible, while being safe. Your answers should not include any harmful, unethical, racist, sexist, toxic, dangerous, or illegal content. Please ensure that your responses are socially unbiased and positive in nature. If a question does not make any sense or is not factually coherent, explain why instead of answering something not correct. If you don't know the answer to a question, please don't share false information.
What is Substrate level phosphorylation in Glycolysis is seen in which step? and explain in detail?
ANSWER: (B) Pyruvate kinaseREF: Murray pp 130-135, Scriver, pp 1521-1552, http://en.wikipedia.org/wiki/Substratelevel_phosphorylationSubstrate-level phosphorylation is a type of metabolism that results in the formation and creation of adenosine triphosphate (ATP) or guanosine triphosphate (GTP) by the direct transfer and donation of a phosphoryi (POa) group to adenosine diphosphate (ADP) or guanosine diphosphate (GDP) from a phosphorylated reactive intermediate. Note that the phosphate group does not have to directly come from the substrate. By convention, the phosphoryl group that is transferred is referred to as a phosphate groupWe use the term substrate level phosphorylation because ATP formation in glycolysis is powered by the addition/ removal of phosphate groups (phosphorylation) to molecules of glucose (the substrate). Hence the term substrate-level phosphorylation.Examples of substrate level phosphorylation that occurs in glycolysis is:The production of 1st ATP when 1,3 bisphophoglycerate is converted into 3-phosphoglycerate by the enzyme phosphoglycerate kinaseProduction of 2nd ATP when phosphoenolpyruvate is converted to pyruvate by the enzyme pyruvate kinase.