are small, irregular, dark-staining granules that appear near the periphery of a young erythrocyte in a Wright stain. Polychromatophilic red cells - young red cells that no longer have nucleus but still contain some RNA. Cabot rings - ring-like structure and may appear in erythrocytes in megaloblastic anemia or in severe anemias, lead poisoning, and in dyserythropoiesis, in which erythrocytes are destroyed before being released from the bone marrow. Abnormal hemoglobin precipitation Heinz bodies - round bodies, refractile inclusions not visible on a Wright's stain film. They are best identified by supravital staining with basic dyes. Hemoglobin H inclusions - alpha thalassemia, greenish-blue inclusion bodies appear in many erythrocytes after four drops of blood is incubated with 0.5mL of Brilliant cresyl blue for 20 minutes at 37 °C. Protozoan inclusion Malaria Babesia In white blood cells Inclusions of immunoglobulin called Russell bodies are found in atypical plasma cells. Russell bodies clump together in large numbers displacing the cell nucleus to the edge, and the cell is then called a Mott cell. In viruses Examples of viral inclusion bodies in animals are Cytoplasmic eosinophilic (acidophilic)- Downie bodies in cowpox Negri bodies in rabies Guarnieri bodies in vaccinia, variola (smallpox) Paschen bodies in variola (smallpox) Bollinger bodies in fowlpox Molluscum bodies in Molluscum contagiosum Eosinophilic inclusion bodies in boid inclusion body disease Nuclear eosinophilic (acidophilic)- Cowdry bodies type A in Herpes simplex virus and Varicella zoster virus Torres bodies in yellow fever Cowdry bodies type B in polio and adenovirus Nuclear basophilic- Cowdry bodies type B in adenovirus "Owl's eye appearance" in cytomegalovirus Both nuclear and cytoplasmic- Warthin–Finkeldey bodies in measles and HIV/AIDS Examples of viral inclusion bodies in plants include aggregations of virus particles (like those for Cucumber mosaic virus) and aggregations of viral proteins (like the cylindrical inclusions of potyviruses). Depending on the plant and the plant virus family these inclusions can be found in epidermal cells, mesophyll cells, and stomatal cells when plant tissue is properly stained. In bacteria Polyhydroxyalkanoates (PHA) are produced by bacteria as inclusion bodies. The size of PHA granules are limited in E. coli, due to its small size. Bacterial cell's inclusion bodies are not as abundant intracellularly, in comparison to eukaryotic cells. Isolation of proteins Between 70% and 80% of recombinant proteins expressed E. coli are contained in inclusion bodies (i.e., protein aggregates). The purification of the expressed proteins from inclusion bodies usually require