Snuppy (Korean: 스너피 a portmanteau of "SNU" and "puppy"; April 24, 2005 – May 2015) was an Afghan hound, the first dog clone. The puppy was created using a cell from an ear from an adult Afghan hound and involved 123 surrogate mothers, of which only two produced pups (Snuppy being the sole survivor). The Department of Theriogenology and Biotechnology at Seoul National University, which cloned Snuppy, was led by Woo Suk Hwang. Snuppy has since been used in the first known successful breeding between cloned canines after his sperm was used to artificially inseminate two cloned females, which resulted in the birth of 10 puppies in 2008. In 2017, 4 clones of Snuppy were made by Sooam, and were the first clones made of a cloned dog, to investigate potential health effects of cloning. History After Dolly the sheep was cloned in 1996, scientists had managed to clone numerous other animals, including cats, cows, gaur, horses, mice, mules, pigs, rabbits and rats but had been unsuccessful in cloning a dog due to the problematic task of maturing a canine ovum in an artificial environment. After several failed attempts by other scientists, Woo Suk Hwang, a lead researcher at Seoul National University, created a clone using tissue from the ear of a 3-year-old Afghan hound. 123 surrogate mothers were used to carry the embryos, of which 1,095 were implanted, the procedure resulted in only three pregnancies; one resulted in a miscarriage, the other pup was born successfully but died of pneumonia three weeks after birth, the successful clone was carried by a Labrador Retriever. From the original 1,095 embryos to the final two puppies, this placed the success rate of the project at less than two tenths of a percent. Snuppy was named as a portmanteau of the initials of the Seoul National University (SNU) and the word "puppy". Process As the eggs in a female canine are only fertile during the estrus phase of the estrous cycle, the eggs could only be harvested during a three-week period each year. Due to complexities with removing eggs from canine ovaries the eggs had to be extracted from the oviduct, which required constant monitoring to achieve. The nucleus of each egg was replaced with the cell from the ear of the adult dog and then electrified and fused using a chemical reaction. The embryos were then transferred to the surrogate dogs. Three