name
stringclasses 1
value | host_cell
stringclasses 1
value | host_strain
stringclasses 1
value | system_type
stringclasses 1
value | assay_type
stringclasses 1
value | temperature
float64 30
30
| ec_number
stringclasses 1
value | spmdb_ac
stringlengths 13
15
| mutations
stringlengths 3
5
| aa_seq
stringlengths 392
392
| label
float64 -0.67
14.9
|
|---|---|---|---|---|---|---|---|---|---|---|
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.90S
|
N90S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHSEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.247552 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.63S
|
C63S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRISNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.010343 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.271F
|
T271F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCFKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.072191 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.32Q
|
H32Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFQWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.11503 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.21Y
|
K21Y
|
MKLENGQKFGRVHERAEGPAYILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.336623 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.33M
|
W33M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHMVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.270239 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.299R
|
N299R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWRSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.005491 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.126V
|
K126V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSVITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.01417 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.381G
|
T381G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLGIETVVLRSVPI
| 0.445331 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.64G
|
N64G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICGRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.042105 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.314H
|
D314H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVHQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.233424 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.262N
|
R262N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLNEMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.172022 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.315G
|
Q315G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDGVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.093298 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.319C
|
T319C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESCLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.077808 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.315P
|
Q315P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDPVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.215253 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.257T
|
L257T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHTALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.872804 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.304F
|
I304F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWFLHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.08433 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.378E
|
P378E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGEGLTIETVVLRSVPI
| 0.598479 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.108N
|
L108N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKNGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.049011 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.287W
|
I287W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLWKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.529424 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.149Y
|
K149Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIYLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.375828 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.38F
|
S38F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSFYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.158052 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.38G
|
S38G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSGYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.138473 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.58L
|
K58L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKELFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.098812 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.261Q
|
L261Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHQREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.593501 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.44P
|
F44P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYPRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.542609 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.252K
|
N252K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPKGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.510867 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.372R
|
V372R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGRLLSFGPGLTIETVVLRSVPI
| 0.964688 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.236S
|
E236S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLSRPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.61058 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.83G
|
N83G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKGPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.0141 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.262L
|
R262L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLLEMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.063793 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.108W
|
L108W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKWGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.0279 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.109E
|
G109E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLEKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.078855 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.97A
|
R97A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVAQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.303739 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.273N
|
D273N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKNVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.104913 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.328P
|
Q328P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLPATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.06496 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.30C
|
P30C
|
MKLENGQKFGRVHERAEGPAKILAIGTATCFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.072448 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.245K
|
T245K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTKAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.314038 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.245G
|
T245G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTGAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.20991 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.106Q
|
P106Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIQKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 1.34372 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.296F
|
S296F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGIFDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.153431 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.78F
|
E78F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEFILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.099873 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.289D
|
A289D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKDFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.193584 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.286E
|
L286E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCEIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.407125 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.241H
|
E241H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFHIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.517199 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.315C
|
Q315C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDCVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.077322 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.125M
|
S125M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKMKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.375489 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.274L
|
V274L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDLPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.469169 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.255M
|
C255M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDMHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.095888 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.296T
|
S296T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGITDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.034131 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.72G
|
H72G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRGMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.063663 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.286H
|
L286H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCHIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.395338 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.379N
|
G379N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPNLTIETVVLRSVPI
| -0.287629 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.269V
|
H269V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFVCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.252491 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.285E
|
C285E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESELIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.537408 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.75F
|
L75F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFFTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.176181 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.49K
|
S49K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNKDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.115507 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.242K
|
I242K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEKVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.381219 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.100W
|
I100W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDWLVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.184846 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.96Y
|
V96Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDYRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.242824 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.33N
|
W33N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHNVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.222562 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.144V
|
D144V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGAVYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.073112 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.238S
|
P238S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERSIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.508066 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.52W
|
L52W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHWVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.10031 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.22N
|
I22N
|
MKLENGQKFGRVHERAEGPAKNLAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.365189 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.106H
|
P106H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIHKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.977119 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.96T
|
V96T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDTRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.108954 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.308R
|
G308R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPRGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.04967 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.34L
|
V34L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWLDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.060412 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.50V
|
D50V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSVHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.222993 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.84C
|
P84C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNCNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.397931 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.129R
|
H129R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITRLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.116256 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.105K
|
I105K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEKPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.079876 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.110N
|
K110N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGNEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.354256 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.138P
|
V138P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGPDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.479695 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.108T
|
L108T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKTGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.050954 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.147S
|
L147S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQSIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.183684 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.305F
|
L305F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWIFHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.055449 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.343V
|
A343V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSVCVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.246803 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.105D
|
I105D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEDPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.233637 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.127K
|
I127K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKKTHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.525033 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.314G
|
D314G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVGQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.159977 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.368V
|
L368V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGVDLGVLLSFGPGLTIETVVLRSVPI
| -0.020716 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.284G
|
S284G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVEGCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.036629 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.141Y
|
P141Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMYGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.171291 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.271C
|
T271C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCCKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.00346 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.279W
|
A279W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIWKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.282803 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.275R
|
P275R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVRPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.018326 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.346C
|
L346C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVCFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.150845 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.290D
|
F290D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKADEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.012125 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.29A
|
T29A
|
MKLENGQKFGRVHERAEGPAKILAIGTAAPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.026192 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.113A
|
V113A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAALMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.411569 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.68C
|
I68C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMCSKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.040071 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.386E
|
V386E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVELRSVPI
| 0.447236 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.272I
|
K272I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTIDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.327223 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.21G
|
K21G
|
MKLENGQKFGRVHERAEGPAGILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.148133 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.388M
|
R388M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLMSVPI
| -0.547998 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.299Y
|
N299Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWYSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.195369 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.17W
|
E17W
|
MKLENGQKFGRVHERAWGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.276382 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.295S
|
I295S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGSSDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.180623 |
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