name
stringclasses 1
value | host_cell
stringclasses 1
value | host_strain
stringclasses 1
value | system_type
stringclasses 1
value | assay_type
stringclasses 1
value | temperature
float64 30
30
| ec_number
stringclasses 1
value | spmdb_ac
stringlengths 13
15
| mutations
stringlengths 3
5
| aa_seq
stringlengths 392
392
| label
float64 -0.67
14.9
|
|---|---|---|---|---|---|---|---|---|---|---|
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.356T | S356T | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKTGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.250916 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.90D | N90D | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHDEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.125058 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.379S | G379S | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPSLTIETVVLRSVPI | 0.087262 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.295V | I295V | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGVSDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.363294 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.13K | H13K | MKLENGQKFGRVKERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.085004 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.262D | R262D | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLDEMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 2.66497 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.312R | I312R | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNARVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.084012 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.303L | W303L | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFLILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.034978 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.99Y | D99Y | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQYILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.078344 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.244Y | T244Y | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVYTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.015155 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.76C | T76C | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLCEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.395715 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.16Q | A16Q | MKLENGQKFGRVHERQEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.351176 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.122K | Q122K | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAKPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.140124 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.14C | E14C | MKLENGQKFGRVHCRAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.010241 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.299E | N299E | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWESIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.447594 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.87F | C87F | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLFSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.057412 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.361R | L361R | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGRKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.313325 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.354T | K354T | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRTKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.271298 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.102A | V102A | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILASEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.12241 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.32M | H32M | MKLENGQKFGRVHERAEGPAKILAIGTATPFMWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.176181 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.99W | D99W | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQWILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.184136 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.329T | A329T | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQTTRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.050131 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.324L | P324L | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLELNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.256966 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.264Q | M264Q | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREQGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 1.2509 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.148H | I148H | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLHKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.207271 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.382F | I382F | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTFETVVLRSVPI | 0.247811 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.8C | K8C | MKLENGQCFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.359931 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.273M | D273M | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKMVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.074228 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.93Y | S93Y | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPYFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.256863 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.116G | A116G | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMGIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.001804 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.94D | F94D | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSDDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.408442 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.366R | D366R | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGRGLDLGVLLSFGPGLTIETVVLRSVPI | -0.233531 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.129V | H129V | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITVLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.336117 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.140E | M140E | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDEPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.198641 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.118A | D118A | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIAEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.189372 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.334K | L334K | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNIKREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.268109 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.134M | T134M | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCMRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.268566 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.240D | F240D | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIDEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.163174 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.272A | K272A | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTADVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.227225 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.254Q | D254Q | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGQCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.208863 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.359F | E359F | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGRFGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.854975 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.24G | A24G | MKLENGQKFGRVHERAEGPAKILGIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.315036 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.19H | P19H | MKLENGQKFGRVHERAEGHAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.077449 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.249I | F249I | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTIVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.859868 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.262Q | R262Q | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLQEMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.664513 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.256D | H256D | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCDLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.174466 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.337P | Y337P | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREPGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.317497 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.342C | S342C | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSCACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.216286 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.349Y | L349Y | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFIYDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.007114 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.151G | L151G | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLGGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.335104 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.14A | E14A | MKLENGQKFGRVHARAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.024273 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.27E | T27E | MKLENGQKFGRVHERAEGPAKILAIGEATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.310272 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.66H | T66H | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRHMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.479958 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.238N | P238N | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERNIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.191847 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.302G | F302G | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIGWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.134743 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.58Y | K58Y | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEYFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.230377 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.121H | A121H | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWHQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.606919 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.250Y | V250Y | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFYPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.076042 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.279G | A279G | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIGKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.006494 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.118P | D118P | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIPEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.335104 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.293A | L293A | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPAGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.01643 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.96C | V96C | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDCRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.159498 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.237G | R237G | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLEGPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.037472 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.343D | A343D | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSDCVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.194922 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.77L | E77L | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTLEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.573032 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.63V | C63V | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRIVNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.149785 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.31E | F31E | MKLENGQKFGRVHERAEGPAKILAIGTATPEHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.189454 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.386M | V386M | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVMLRSVPI | 0.258234 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.361G | L361G | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGGKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.265416 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.19F | P19F | MKLENGQKFGRVHERAEGFAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.137558 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.259M | L259M | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLAMHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.185257 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.98L | Q98L | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRLDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.187748 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.16S | A16S | MKLENGQKFGRVHERSEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.082805 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.266A | L266A | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGATFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.640005 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.100T | I100T | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDTLVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.022072 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.44S | F44S | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYSRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.226545 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.375W | S375W | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLWFGPGLTIETVVLRSVPI | -0.328155 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.73S | M73S | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHSFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.041135 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.365V | G365V | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSVDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.182041 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.124N | K124N | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPNSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.006288 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.386I | V386I | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVILRSVPI | -0.033997 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.289K | A289K | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKKFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.51401 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.291Q | E291Q | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFQPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.048286 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.375I | S375I | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLIFGPGLTIETVVLRSVPI | -0.555085 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.24E | A24E | MKLENGQKFGRVHERAEGPAKILEIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.438756 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.386K | V386K | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVKLRSVPI | 1.1604 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.336Y | E336Y | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILRYYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.594998 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.48T | N48T | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTTSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.188548 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.342Y | S342Y | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSYACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.559535 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.114H | L114H | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVHMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.201646 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.352K | I352K | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEKRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.562426 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.326L | K326L | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNLLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.426407 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.362S | K362S | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLSTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.012194 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.267D | T267D | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLDFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 1.68757 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.388W | R388W | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLWSVPI | -0.50421 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.46A | V46A | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRATNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | 0.10244 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.29E | T29E | MKLENGQKFGRVHERAEGPAKILAIGTAEPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.101245 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.282T | V282T | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNTESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.564695 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.375N | S375N | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLNFGPGLTIETVVLRSVPI | 0.23619 |
A0A3G4RHW3 | E. coli | BL21(DE3) | IN_VIVO | GFP+FACS | 30 | 2.3.1.74 | WH.GFP.PKS.346I | L346I | MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVIFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI | -0.193638 |
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