name
stringclasses 1
value | host_cell
stringclasses 1
value | host_strain
stringclasses 1
value | system_type
stringclasses 1
value | assay_type
stringclasses 1
value | temperature
float64 30
30
| ec_number
stringclasses 1
value | spmdb_ac
stringlengths 13
15
| mutations
stringlengths 3
5
| aa_seq
stringlengths 392
392
| label
float64 -0.67
14.9
|
---|---|---|---|---|---|---|---|---|---|---|
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.273H
|
D273H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKHVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.159162 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.371A
|
G371A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLAVLLSFGPGLTIETVVLRSVPI
| -0.315463 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.385P
|
V385P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETPVLRSVPI
| 1.89688 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.382R
|
I382R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTRETVVLRSVPI
| 0.67702 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.383I
|
E383I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIITVVLRSVPI
| -0.532869 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.355R
|
K355R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKRSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.165218 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.131R
|
V131R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLRFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.299722 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.36N
|
Q36N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDNSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.080577 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.296C
|
S296C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGICDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.114194 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.32Y
|
H32Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFYWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.04594 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.150N
|
L150N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKNLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.015155 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.50N
|
D50N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSNHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.169069 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.23T
|
L23T
|
MKLENGQKFGRVHERAEGPAKITAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.357798 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.277V
|
T277V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPVIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.00415 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.115G
|
M115G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLGAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.308165 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.373D
|
L373D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVDLSFGPGLTIETVVLRSVPI
| 0.429886 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.26Y
|
G26Y
|
MKLENGQKFGRVHERAEGPAKILAIYTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.564302 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.367S
|
G367S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDSLDLGVLLSFGPGLTIETVVLRSVPI
| -0.079111 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.14R
|
E14R
|
MKLENGQKFGRVHRRAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.00677 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.309P
|
G309P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGPNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.089961 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.101G
|
L101G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDIGVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.158694 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.330S
|
T330S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQASRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.058705 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.97M
|
R97M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVMQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.040669 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.265W
|
G265W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMWLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.375272 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.364A
|
S364A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTAGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.245287 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.312V
|
I312V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAVVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.053215 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.303Q
|
W303Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFQILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.024104 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.337N
|
Y337N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILRENGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.332652 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.373H
|
L373H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVHLSFGPGLTIETVVLRSVPI
| 0.307853 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.19I
|
P19I
|
MKLENGQKFGRVHERAEGIAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.094115 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.123H
|
P123H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQHKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.121996 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.380S
|
L380S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGSTIETVVLRSVPI
| 1.0202 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.371K
|
G371K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLKVLLSFGPGLTIETVVLRSVPI
| -0.247585 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.340I
|
L340I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNISSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.007444 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.327S
|
L327S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKSQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.057898 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.130V
|
L130V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHVVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.211654 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.314T
|
D314T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVTQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.474996 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.312K
|
I312K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAKVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.453611 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.374R
|
L374R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLRSFGPGLTIETVVLRSVPI
| 1.84632 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.252L
|
N252L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPLGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.212261 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.298E
|
W298E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDENSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.248836 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.61I
|
R61I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRIICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.455766 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.277C
|
T277C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPCIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.228161 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.384N
|
T384N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIENVVLRSVPI
| -0.122543 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.274S
|
V274S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDSPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.025196 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.113E
|
V113E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAELMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.117664 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.112I
|
A112I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEIVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.375489 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.357A
|
G357A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSAREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.674929 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.32W
|
H32W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFWWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.227736 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.297E
|
D297E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISEWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.003598 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.350I
|
D350I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILIEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.594998 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.238C
|
P238C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERCIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.230763 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.305N
|
L305N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWINHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.110801 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.27V
|
T27V
|
MKLENGQKFGRVHERAEGPAKILAIGVATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.081915 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.60H
|
R60H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFHRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.646269 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.79W
|
I79W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEWLQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.077513 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.297G
|
D297G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISGWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.114785 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.285K
|
C285K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESKLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.291323 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.261G
|
L261G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHGREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.089601 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.325L
|
N325L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPLKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.1716 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.334Q
|
L334Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNIQREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.12701 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.343I
|
A343I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSICVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.53261 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.292D
|
P292D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEDLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.172919 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.110L
|
K110L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGLEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.019951 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.349H
|
L349H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFIHDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.056562 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.377K
|
G377K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFKPGLTIETVVLRSVPI
| 0.971919 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.344R
|
C344R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSARVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.272266 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.260T
|
H260T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALTLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.079153 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.372T
|
V372T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGTLLSFGPGLTIETVVLRSVPI
| 0.727876 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.370K
|
L370K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDKGVLLSFGPGLTIETVVLRSVPI
| 0.130296 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.254N
|
D254N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGNCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.045908 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.329S
|
A329S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQSTRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.157412 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.381H
|
T381H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLHIETVVLRSVPI
| 0.199888 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.40S
|
P40S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYSDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.274472 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.332C
|
N332C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRCILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.168696 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.44L
|
F44L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYLRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.025793 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.347H
|
F347H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLHILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.5208 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.269S
|
H269S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFSCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.006676 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.299I
|
N299I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWISIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.165028 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.16Y
|
A16Y
|
MKLENGQKFGRVHERYEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.080276 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.293V
|
L293V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPVGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.291618 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.364C
|
S364C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTCGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.31821 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.145Q
|
Y145Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADQQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.323722 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.130F
|
L130F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHFVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.139707 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.385D
|
V385D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETDVLRSVPI
| 1.23938 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.263F
|
E263F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLRFMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.058456 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.329W
|
A329W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQWTRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.285552 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.276Y
|
P276Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPYTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.340341 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.63W
|
C63W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRIWNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.375065 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.336N
|
E336N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILRNYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.421295 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.37G
|
S37G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQGSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.310176 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.368D
|
L368D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGDDLGVLLSFGPGLTIETVVLRSVPI
| 1.03468 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.350W
|
D350W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILWEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.427598 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.117M
|
I117M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAMDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.512149 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.368I
|
L368I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGIDLGVLLSFGPGLTIETVVLRSVPI
| -0.590283 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.12D
|
V12D
|
MKLENGQKFGRDHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.001662 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.291A
|
E291A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFAPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.04027 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.283A
|
E283A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVASCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.046158 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.148Y
|
I148Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLYKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.187807 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.91P
|
E91P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNPPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.190614 |
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