name
stringclasses 1
value | host_cell
stringclasses 1
value | host_strain
stringclasses 1
value | system_type
stringclasses 1
value | assay_type
stringclasses 1
value | temperature
float64 30
30
| ec_number
stringclasses 1
value | spmdb_ac
stringlengths 13
15
| mutations
stringlengths 3
5
| aa_seq
stringlengths 392
392
| label
float64 -0.67
14.9
|
---|---|---|---|---|---|---|---|---|---|---|
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.238V
|
P238V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERVIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.276984 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.346M
|
L346M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVMFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.202303 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.383L
|
E383L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTILTVVLRSVPI
| -0.421696 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.144H
|
D144H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGAHYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.164653 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.83D
|
N83D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKDPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.287777 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.134I
|
T134I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCIRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.155887 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.141C
|
P141C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMCGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.401427 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.24S
|
A24S
|
MKLENGQKFGRVHERAEGPAKILSIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.150492 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.90F
|
N90F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHFEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 1.39612 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.91Q
|
E91Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNQPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.253794 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.339H
|
N339H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGHLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.304414 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.281H
|
N281H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKHVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.706807 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.246S
|
A246S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTSQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.640232 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.20G
|
A20G
|
MKLENGQKFGRVHERAEGPGKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.300692 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.133S
|
C133S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFSTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.201646 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.359D
|
E359D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGRDGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.019765 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.261P
|
L261P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHPREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.303057 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.125G
|
S125G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKGKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.26523 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.297S
|
D297S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISSWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.065673 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.333E
|
I333E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNELREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.294264 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.55P
|
L55P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDPKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.021933 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.249A
|
F249A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTAVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.143812 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.134W
|
T134W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCWRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.109863 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.56L
|
K56L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLLEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.103671 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.328L
|
Q328L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLLATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.055993 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.346P
|
L346P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVPFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.12701 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.350Q
|
D350Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILQEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.493477 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.312S
|
I312S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNASVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.233637 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.324V
|
P324V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEVNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.013425 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.143S
|
A143S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGSDYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.061276 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.327C
|
L327C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKCQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.108628 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.95M
|
D95M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFMVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.383766 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.78Q
|
E78Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEQILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.268515 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.387N
|
L387N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVNRSVPI
| 0.663705 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.69A
|
S69A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMIAKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.154686 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.322Y
|
L322Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGYEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.514683 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.274P
|
V274P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDPPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.044491 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.57K
|
E57K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKKKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.143059 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.292T
|
P292T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFETLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.038139 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.382H
|
I382H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTHETVVLRSVPI
| 0.306946 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.328T
|
Q328T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLTATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.113004 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.70S
|
K70S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISSRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.312484 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.370Q
|
L370Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDQGVLLSFGPGLTIETVVLRSVPI
| 2.46511 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.69C
|
S69C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMICKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.270288 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.49L
|
S49L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNLDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.025267 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.273W
|
D273W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKWVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.024915 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.358F
|
R358F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGFEGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.421295 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.296K
|
S296K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGIKDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.167967 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.300D
|
S300D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNDIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 1.07671 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.377Q
|
G377Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFQPGLTIETVVLRSVPI
| 0.580411 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.80D
|
L80D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEIDQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.551001 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.286K
|
L286K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCKIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.132161 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.307V
|
P307V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHVGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.426089 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.263Q
|
E263Q
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLRQMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.066586 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.349W
|
L349W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFIWDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.082487 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.95H
|
D95H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFHVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.002838 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.131L
|
V131L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLLFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.360277 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.78P
|
E78P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEPILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.424544 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.328E
|
Q328E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLEATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.339377 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.388V
|
R388V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLVSVPI
| -0.457874 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.40H
|
P40H
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYHDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.387577 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.310Y
|
N310Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGYAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.637165 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.97V
|
R97V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVVQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.277302 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.288G
|
K288G
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIGAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.045505 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.47R
|
T47R
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVRNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.030611 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.270T
|
C270T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHTTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 2.03627 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.311P
|
A311P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNPIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.03627 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.71M
|
R71M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKMHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.222993 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.80P
|
L80P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEIPQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.206309 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.358E
|
R358E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGEEGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.026333 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.287V
|
I287V
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLVKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.423853 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.64S
|
N64S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICSRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.153841 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.119M
|
E119M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDMWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.163116 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.47L
|
T47L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVLNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.085218 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.87W
|
C87W
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLWSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.087036 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.93F
|
S93F
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPFFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.04306 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.287E
|
I287E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLEKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.117542 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.92S
|
P92S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNESSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.091642 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.366E
|
D366E
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGEGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.307581 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.89K
|
H89K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSKNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.442429 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.345Y
|
V345Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACYLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.464633 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.241T
|
E241T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFTIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.177968 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.387M
|
L387M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVMRSVPI
| 0.120467 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.386C
|
V386C
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVCLRSVPI
| 0.212765 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.116S
|
A116S
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMSIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.057412 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.388K
|
R388K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLKSVPI
| 0.294863 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.269T
|
H269T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFTCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.06238 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.138K
|
V138K
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGKDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.09954 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.19M
|
P19M
|
MKLENGQKFGRVHERAEGMAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.094931 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.75M
|
L75M
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFMTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.176181 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.86A
|
L86A
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNACSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.459562 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.388D
|
R388D
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLDSVPI
| 0.193915 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.340T
|
L340T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNTSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.020446 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.242Y
|
I242Y
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEYVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.070453 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.322I
|
L322I
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGIEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.06897 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.257N
|
L257N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHNALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 1.31193 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.388N
|
R388N
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLNSVPI
| -0.629383 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.113L
|
V113L
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEALLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| 0.065404 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.62P
|
I62P
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRPCNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAIVDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.509475 |
A0A3G4RHW3
|
E. coli
|
BL21(DE3)
|
IN_VIVO
|
GFP+FACS
| 30 |
2.3.1.74
|
WH.GFP.PKS.313T
|
V313T
|
MKLENGQKFGRVHERAEGPAKILAIGTATPFHWVDQSSYPDYYFRVTNSDHLVDLKEKFRRICNRTMISKRHMFLTEEILQKNPNLCSHNEPSFDVRQDILVSEIPKLGKEAVLMAIDEWAQPKSKITHLVFCTRSGVDMPGADYQLIKLLGLSPSVQRVMMYQQGCFAGGTMLRLAKDLAENNKGARVLVVCAESSAIGFRGPSEDHPDNLIAQALFGDGAAALIIGSDPKMGLERPIFEIVTTAQTFVPNGDCHLALHLREMGLTFHCTKDVPPTIAKNVESCLIKAFEPLGISDWNSIFWILHPGGNAITDQVESTLGLEPNKLQATRNILREYGNLSSACVLFILDEIRKKSGREGLKTSGDGLDLGVLLSFGPGLTIETVVLRSVPI
| -0.037472 |
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