Reactive oxygen species, such as superoxide ion (O−
2) and hydrogen peroxide (H
2O
2), are dangerous by-products of oxygen use in organisms. Parts of the immune system of higher organisms create peroxide, superoxide, and singlet oxygen to destroy invading microbes. Reactive oxygen species also play an important role in the hypersensitive response of plants against pathogen attack. Oxygen is toxic to obligately anaerobic organisms, which were the dominant form of early life on Earth until O
2 began to accumulate in the atmosphere about 2.5 billion years ago during the Great Oxygenation Event, about a billion years after the first appearance of these organisms.