Spaces:
Sleeping
Sleeping
foz
commited on
Commit
·
8562cb9
1
Parent(s):
1d89ca0
Docker container
Browse files- Dockerfile +29 -0
- app_local.py +81 -0
Dockerfile
ADDED
|
@@ -0,0 +1,29 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
# https://huggingface.co/spaces/sayakpaul/demo-docker-gradio/blob/main/Dockerfile
|
| 2 |
+
|
| 3 |
+
FROM python:3.9
|
| 4 |
+
|
| 5 |
+
WORKDIR /code
|
| 6 |
+
|
| 7 |
+
COPY *.py /code/
|
| 8 |
+
|
| 9 |
+
COPY ./requirements.txt /code/requirements.txt
|
| 10 |
+
|
| 11 |
+
RUN pip install --no-cache-dir --upgrade -r /code/requirements.txt
|
| 12 |
+
|
| 13 |
+
# Set up a new user named "user" with user ID 1000
|
| 14 |
+
RUN useradd -m -u 1000 user
|
| 15 |
+
|
| 16 |
+
# Switch to the "user" user
|
| 17 |
+
USER user
|
| 18 |
+
|
| 19 |
+
# Set home to the user's home directory
|
| 20 |
+
ENV HOME=/home/user \
|
| 21 |
+
PATH=/home/user/.local/bin:$PATH
|
| 22 |
+
|
| 23 |
+
# Set the working directory to the user's home directory
|
| 24 |
+
WORKDIR $HOME/app
|
| 25 |
+
|
| 26 |
+
# Copy the current directory contents into the container at $HOME/app setting the owner to the user
|
| 27 |
+
COPY --chown=user . $HOME/app
|
| 28 |
+
|
| 29 |
+
CMD ["python", "app_local.py"]
|
app_local.py
ADDED
|
@@ -0,0 +1,81 @@
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 1 |
+
|
| 2 |
+
import gradio as gr
|
| 3 |
+
from tifffile import imread
|
| 4 |
+
from PIL import Image
|
| 5 |
+
from path_analysis.analyse import analyse_paths
|
| 6 |
+
import numpy as np
|
| 7 |
+
|
| 8 |
+
|
| 9 |
+
# Function to preview the imported image
|
| 10 |
+
def preview_image(file1):
|
| 11 |
+
if file1:
|
| 12 |
+
print('Uploading image', file1.name)
|
| 13 |
+
im = imread(file1.name)
|
| 14 |
+
print(im.ndim, im.shape)
|
| 15 |
+
if im.ndim>2:
|
| 16 |
+
return Image.fromarray(np.max(im, axis=0))
|
| 17 |
+
else:
|
| 18 |
+
return Image.fromarray(im)
|
| 19 |
+
else:
|
| 20 |
+
return None
|
| 21 |
+
|
| 22 |
+
|
| 23 |
+
with gr.Blocks() as demo:
|
| 24 |
+
with gr.Row():
|
| 25 |
+
with gr.Column():
|
| 26 |
+
# Inputs for cell ID, image, and path
|
| 27 |
+
cellid_input = gr.Textbox(label="Cell ID", placeholder="Image_1")
|
| 28 |
+
image_input = gr.File(label="Input foci image")
|
| 29 |
+
image_preview = gr.Image(label="Max projection of foci image")
|
| 30 |
+
image_input.change(fn=preview_image, inputs=image_input, outputs=image_preview)
|
| 31 |
+
path_input = gr.File(label="SNT traces file")
|
| 32 |
+
|
| 33 |
+
# Additional options wrapped in an accordion for better UI experience
|
| 34 |
+
with gr.Accordion("Additional options ..."):
|
| 35 |
+
sphere_radius = gr.Number(label="Trace sphere radius (um)", value=0.1984125, interactive=True)
|
| 36 |
+
peak_threshold = gr.Number(label="Peak relative threshold", value=0.4, interactive=True)
|
| 37 |
+
# Resolutions for xy and z axis
|
| 38 |
+
with gr.Row():
|
| 39 |
+
xy_res = gr.Number(label='xy-yesolution (um)', value=0.0396825, interactive=True)
|
| 40 |
+
z_res = gr.Number(label='z resolution (um)', value=0.0909184, interactive=True)
|
| 41 |
+
# Resolutions for xy and z axis
|
| 42 |
+
|
| 43 |
+
threshold_type = gr.Radio(["per-trace", "per-cell"], label="Threshold-type", value="per-trace", interactive=True)
|
| 44 |
+
use_corrected_positions = gr.Checkbox(label="Correct foci position measurements", value=True, interactive=True)
|
| 45 |
+
screening_distance = gr.Number(label='Screening distance (voxels)', value=10, interactive=True)
|
| 46 |
+
|
| 47 |
+
|
| 48 |
+
# The output column showing the result of processing
|
| 49 |
+
with gr.Column():
|
| 50 |
+
trace_output = gr.Image(label="Overlayed paths")
|
| 51 |
+
image_output=gr.Gallery(label="Traced paths")
|
| 52 |
+
plot_output=gr.Plot(label="Foci intensity traces")
|
| 53 |
+
data_output=gr.DataFrame(label="Detected peak data")#, "Peak 1 pos", "Peak 1 int"])
|
| 54 |
+
data_file_output=gr.File(label="Output data file (.csv)")
|
| 55 |
+
|
| 56 |
+
|
| 57 |
+
def process(cellid_input, image_input, path_input, sphere_radius, peak_threshold, xy_res, z_res, threshold_type, use_corrected_positions, screening_distance):
|
| 58 |
+
|
| 59 |
+
config = { 'sphere_radius': sphere_radius,
|
| 60 |
+
'peak_threshold': peak_threshold,
|
| 61 |
+
'xy_res': xy_res,
|
| 62 |
+
'z_res': z_res,
|
| 63 |
+
'threshold_type': threshold_type,
|
| 64 |
+
'use_corrected_positions': use_corrected_positions,
|
| 65 |
+
'screening_distance': screening_distance,
|
| 66 |
+
}
|
| 67 |
+
|
| 68 |
+
|
| 69 |
+
paths, traces, fig, extracted_peaks = analyse_paths(cellid_input, image_input.name, path_input.name, config)
|
| 70 |
+
extracted_peaks.to_csv('output.csv')
|
| 71 |
+
print('extracted', extracted_peaks)
|
| 72 |
+
return paths, [Image.fromarray(im) for im in traces], fig, extracted_peaks, 'output.csv'
|
| 73 |
+
|
| 74 |
+
|
| 75 |
+
with gr.Row():
|
| 76 |
+
greet_btn = gr.Button("Process")
|
| 77 |
+
greet_btn.click(fn=process, inputs=[cellid_input, image_input, path_input, sphere_radius, peak_threshold, xy_res, z_res, threshold_type, use_corrected_positions, screening_distance], outputs=[trace_output, image_output, plot_output, data_output, data_file_output], api_name="process")
|
| 78 |
+
|
| 79 |
+
|
| 80 |
+
if __name__ == "__main__":
|
| 81 |
+
demo.launch(server_name="0.0.0.0", share=False)
|