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---
title: De Novo Peptide Sequencing With InstaNovo and InstaNovo+
emoji: 📊
colorFrom: green
colorTo: green
sdk: gradio
sdk_version: 5.23.1
app_file: app.py
pinned: true
license: apache-2.0
thumbnail: >-
https://cdn-uploads.huggingface.co/production/uploads/6189aee17d9b289cdebafbd6/tb9e-8Z2_pDsRMkGglcvh.png
short_description: Translate fragment ion peaks into sequence of amino acids
---
# _De Novo_ Peptide Sequencing With InstaNovo and InstaNovo+
This Space provides a web interface for the [InstaNovo](https://github.com/instadeepai/InstaNovo) models for _de novo_ peptide sequencing from mass spectrometry data.
**Features:**
* Upload MS/MS data in common formats (`.mgf`, `.mzml`, `.mzxml`).
* Choose between fast Greedy Search or more accurate but slower Knapsack Beam Search.
* View predictions directly in the interface.
* Download full results as a CSV file.
**How to Use:**
1. Upload your mass spectrometry data file.
2. Select the desired decoding method.
3. Click "Predict Sequences".
4. View the results table and download the CSV if needed.
**Model:**
This demo uses the pretrained model checkpoint.
* Predictions use version `instanovo-v1.1.0` for the transformer-based InstaNovo model and version `instanovoplus-v1.1.0-alpha` for the diffusion-based InstaNovo+ model.
* The InstaNovo+ model `instanovoplus-v1.1.0-alpha` is an alpha release.
**Note:** Processing large files can take time, depending on the file size and the chosen decoding method. Knapsack generation can also add to the initial startup time. |