
such as mouse fibroblasts and hepatocytes . Non-neural cells, however, typically require more than one transcription factor to achieve a full neuronal conversion. Recently, it has been reported that expression of Ascl1 alone, but no other proneural genes such as Neurog2, is sufficient to induce conversion of fibroblasts into induced neurons , albeit at low efficiency (~10%).
Studies using different cell types and neurogenic transcription factors describes a significant failure rate in reprogramming .
Generally 
Results & Discussion
To To further confirm that the observed pseudo-temporal map could represent different fates of reprogrammed MEFs, we first analyzed celltype ontologies on Mouse Gene Atlas using enrichR ( Figure B ). We could [13] [ confirm that genes were enriched for MEF, neural cells and muscle cell ontologies ( Figure B) . Next, pan-neuronal markers (Tubb3, Map2), myocyte markers (Tnnc2, Myo18b) and MEF markers (S100a4, Col1a2) cell populations and four genes specifically enriched in ascl1d5 cells in [12] [12] 
Alternative Explanations
Not Applicable.
Conjectures [16] [17] [16] [18]
[19] [20] [21]
[22]
[23]
21/07/2017 It would be interesting to evaluate candidate genes with a system biology approach looking at, for example, gene regulatory networks.
Furthermore, miRNAs plays an important role on regulation of gene networks and had been already used as reprogramming enhancers, so they should not be neglected in the future analysis. 
Methods


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