Introduction
Although it is not necessary in all cases (2,4), post-embedding immunostaining often requires the section to be surface-etched or treated in such a way that the plastic is partially or totally removed from the tissue (6, 9, 12, 15, 18, 22, 24, 25, 26, 31, 39 For electron microscopic studies, grids were always kept covered in moist chambers (petri dishes with moistened filter paper on the bottom) between incubations and washes. Incubations and washes were done on clean pieces of Parafilm or dental wax at room temperature, unless otherwise noted. Grids were transferred between drops with anti-capillary forceps to minimize carry-over of solutions.
Introduction
Droplets of buffer washes were administered by syringes fitted with 0.2 .tm Millipore filters (Millipore; Bedford, MA).
Introduction
The colloidal gold immunostaining protocol was as follows: (a) 10% normal goat serum in 0.05 M Tris-HCI buffered saline (TBS), pH 7.6, 10 mm; (b) 
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