Title of Invention: METHODS OF REPLICATING A LARGE SCALE ECULIZUMAB PRODUCTION CELL CULTURE Abstract: Provided herein are methods of replicating a large scale eculizumab production cell culture in a small scale culture. Detailed Description: RELATED APPLICATIONS This application claims priority to and the benefit of U.S. provisional patent application No. 62/064,457, filed on Oct. 15, 2014, the disclosure of which is incorporated herein by reference in its entirety. SEQUENCE LISTING The instant application contains a Sequence Listing which has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Sep. 25, 2015, is named AXJ-197_SL.txt and is 6,272 bytes in size. TECHNICAL FIELD This invention relates to methods cell culture and the manufacture of recombinant proteins. BACKGROUND Eculizumab is a humanized monoclonal antibody that specifically binds to human complement component 5 (C5). Eculizumab has been approved by the FDA for treating paroxysmal nocturnal hemoglobinuria and atypical hemolytic uremic syndrome. Eculizumab is typically produced using a large scale (10,000-L) fed batch mammalian cell culture. Small-scale models of production cell cultures provide a means for testing culture media or media components and/or mammalian cell lines prior to their use in a large scale production cell culture. Small-scale models of productions cell cultures also provide a means for testing culture media or media components and/or mammalian cell lines for contamination prior to their use in large scale production cultures. There is a need for a small scale model of the eculizumab production process. SUMMARY The present invention is based, at least in part, on the discovery of a set of culture parameters that allows for the accurate replication of a large scale eculizumab production cell culture using a small scale fed batch cell culture. Thus, the present specification includes methods of replicating a large scale eculizumab production cell culture using a small scale fed batch cell culture. Provided herein are methods of replicating a large-scale eculizumab production cell culture in a small scale culture that include: providing a 4-L to 10-L bioreactor (such as a fed batch bioreactor) containing a first culture medium that occupies between about 70% and about 90% of the capacity of the bioreactor and has an initial cell density of between about 2.5×105 and about 7.5×105 NS0 cells/mL, the cells containing a recombinant eculizumab-encoding nucleic acid; culturing (such as fed batch culturing) the cells in the bioreactor for at least 8 days at about 34° C. to about 39° C., at a dO2 level of between about 14% to about 16%, and a rotary agitation of between about 50 RPM and about 70 RPM; maintaining (e.g., continuing) fed batch culturing until a cell density of about 13×105 to about 20×105 cells/mL is reached, then; (1) continuously adding a feed culture medium to the first culture medium for a period of about 120 hours to about 150 hours, and about 40 to 60 hours after the start of continuous addition of the feed culture medium, and (2) continuously adding an alkali base solution to the first culture medium for a period of between about 90 hours to about 105 hours. In some embodiments of any of the methods described herein, one or both of the first culture medium and the feed culture medium includes a processed bovine serum albumin (BSA). In some embodiments of any of the methods described herein, the processed BSA is processed New Zealand BSA. In some embodiments of any of the methods described herein, the initial cell density is between about 4.0×105 cells/mL and about 6.0×106 cells/mL. In some embodiments of any of the methods described herein, the bioreactor has a capacity of between about 4-L to about 6-L. In some embodiments of any of the methods described herein, the first culture medium occupies between about 80% to about 88% of the capacity of the bioreactor. In some embodiments of any of the methods described herein, the fed batch culturing is performed at a temperature of about 36° C. to about 37° C. In some embodiments of any of the methods described herein, the rotary agitation is between about 55 RPM and about 65 RPM. In some embodiments of any of the methods described herein, the target cell density is between about 17×105 cells/mL and about 19×105 cells/mL. In some embodiments of any of the methods described herein, the alkali base solution is continuously added to the first culture medium for a period of between about 92 hours to about 100 hours. In some embodiments of any of the methods described herein, the bioreactor contains 4.2 L of the first culture medium, and the alkali base solution is continuously added to the first culture medium at a rate of between about 1.65 mL/hour to about 1.85 mL/hour. In some embodiments of any of the methods described herein, the alkali base solution includes between about 0.65 M and about 0.85 M sodium carbonate and between about 0.4 M and about 0.6 M sodium bicarbonate. In some embodiments of any of the methods described herein, a single feed culture medium is continuously added to the first culture medium. In some embodiments of any of the methods described herein, two different feed culture media are continuously added to the first culture medium. In some embodiments of any of the methods described herein, each of the two different feed culture media are continuously added to the first culture medium at a rate of between about 15 μL/minute to about 35 μL/minute. In some embodiments of any of the methods described herein, each of the two different feed culture media are continuously added to the first culture medium at a rate of between about 20 μL/minute to about 25 μL/minute. In some embodiments of any of the methods described herein, the feed culture medium is continuously added to the first culture medium for a period of between about 130 hours to about 135 hours. Some embodiments of any of the methods described herein further include adding a bolus of a lipid solution including linoleic acid, oleic acid, and cholesterol to the first culture medium at a time point when the cells reach a density of about 13×105 cells/mL to about 20×105 cells/mL. In some embodiments of any of the methods described herein, the lipid solution is added at a dose of about 1 mL per 1 L of the first culture medium. Some embodiments of any of the methods described herein further include adding anti-foam to the first culture medium during culturing. Some embodiments of any of the methods described herein further include collecting the recombinant eculizumab produced in the culturing step. In some embodiments of any of the methods described herein, the collecting includes lysing the cells. In some embodiments of any of the methods described herein, the recombinant eculizumab is collected from one or both of the first culture medium and the feed culture medium. In some embodiments of any of the methods described herein, eculizumab includes a heavy chain including or consisting of SEQ ID NO: 1 and a light chain including or consisting of SEQ ID NO: 2. Some embodiments of any of the methods described herein further include assessing one of more cell culture parameters selected from the group of: cell growth, percentage cell viability, viable cell density, aerobic glucose consumption, eculizumab titer, specific productivity rate, volumetric productivity rate, lactate production, and eculizumab quality. Some embodiments of any of the methods described herein further include comparing the determined one or more cell culture parameters to the same one or more cell culture parameters in a large-scale eculizumab production cell culture. In some embodiments of any of the methods described herein, the large-scale eculizumab production cell culture is a 10,000 L-eculizumab production cell culture. As used herein, the word “a” or “plurality” before a noun represents one or more of the particular noun. For example, the phrase “a mammalian cell” represents “one or more mammalian cells.” The term “processed BSA” or “processed bovine serum albumin” refers to a bovine serum albumin that has been produced by a method described herein. For example, a method used to produce a processed BSA does not include heating a solution including the serum albumin, adding a stabilizer to a solution including the serum albumin, and precipitating impurities out of a solution that includes a reconstituted lyophilized serum albumin. In some examples, the method used to produce processed BSA starts with the provision of plasma from a bovine. Non-limiting methods that can be used to produce a processed BSA are described herein. The term “processed NZ BSA” or “processed New Zealand BSA” refers to a processed BSA as defined herein that includes a serum albumin from a bovine bred and raised in New Zealand. A non-limiting example of a processed NZ BSA is MP Biomedicals NZ BSA. The term “eculizumab production cell culture” refers to a large-scale culture used to produce recombinant eculizumab primarily to be used for the manufacture of a pharmaceutical composition. A production cell culture can have a volume of at least 500 L, for example, about or at least 1,000 L, about or at least 2,000 L, about or at least 3,000 L, about or at least 4,000 L, about or at least 5,000 L, about or at least 6,000 L, about or at least 7,000 L, about or at least 8,000 L, about or at least 9,000 L, or about or at least 10,000 L. The term “production bioreactor” as used herein refers to a vessel suitable for incubating a mammalian cell culture under conditions sufficient for growth of the mammalian cells in the culture and production of a recombinant protein product by the mammalian cells in the culture. Examples of production bioreactors are known in the art. The term “mammalian cell” refers to any cell from or derived from any mammal including, for example, a human, a hamster, a mouse, a green monkey, a rat, a pig, a cow, a hamster, or a rabbit. In some embodiments, the mammalian cell can be an immortalized cell, a differentiated cell, or an undifferentiated cell. The term “substantially free” as used herein refers to a composition (e.g., a pharmaceutical composition) that is at least or about 90% free, or about 95%, 96%, 97%, 98%, or at least or about 99% free, or about 100% free of a specific substance (e.g., contaminating proteins from a liquid culture medium or from the lysate of a mammalian cell). The term “culturing” or “cell culturing” as used herein refers to maintenance or growth of a mammalian cell in a liquid culture medium under a controlled set of physical conditions. The term “liquid culture medium” or “culture medium” refers to a fluid that contains sufficient nutrients to allow a mammalian cell to grow in the medium in vitro. For example, a liquid culture medium can include one or more of: amino acids (e.g., 20 amino acids), a purine (e.g., hypoxanthine), a pyrimidine (e.g., thymidine), choline, inositol, thiamine, folic acid, biotin, calcium, niacinamide, pyridoxine, riboflavin, thymidine, cyanocobalamin, pyruvate, lipoic acid, magnesium, glucose, sodium, potassium, iron, copper, zinc, selenium, and other necessary trace metals, and sodium bicarbonate. A liquid culture medium may include serum from a mammal. In some instances, a liquid culture medium does not contain serum or another extract from a mammal (a chemically-defined liquid culture medium). A liquid culture medium may contain trace metals, a mammalian growth hormone, and/or a mammalian growth factor. Non-limiting examples of liquid culture medium are described herein and additional examples are known in the art and are commercially available. The term “animal-derived component free liquid culture medium” means a liquid culture medium that does not contain any components (e.g., proteins or serum) derived from an animal. The term “serum-free liquid culture medium” refers to a liquid culture medium that does not contain animal serum. The term “serum-added liquid culture medium” refers to a liquid culture medium that includes animal serum. The term “chemically-defined liquid culture medium” refers to a liquid culture medium in which substantially all of the chemical components are known. For example, a chemically-defined liquid culture medium does not contain fetal bovine serum, bovine serum albumin, or human serum albumin, as these preparations typically contain a complex mix of albumins and lipids. The term “protein-free liquid culture medium” means a liquid culture medium that does not contain any protein (e.g., any detectable protein). “Rotary agitation” is a term well-known in the art and refers to the agitation of a culture in a bioreactor (e.g., a production bioreactor) in a generally circular fashion, e.g., clock-wise or counter-clockwise, in order to, e.g., increase the dissolved O2 concentration in the culture in the bioreactor. Agitation can be performed using any method known in the art, e.g., an instrument that moves the culture in a circular or ellipsoidal motion, such as an impeller. Exemplary devices that can be used to perform rotary agitation are known in the art and are commercially available. The term “purify” or “purifying” in certain contexts means at least partially isolating a recombinant protein from one or more other components (e.g., DNA, RNA, or other proteins) present in the liquid culture medium or cell culture lysate. The extent of purification can be specified, such as at least or about 5%, e.g., at least or about 10%, 15%, 20%, 25%, 30%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, or at least or about 95% to 99.9% pure by weight. Non-limiting methods for purifying a protein from a liquid culture medium or from a mammalian cell lysate are described herein and others are known in the art. The term “fed batch cell culture” or “fed batch culturing” means the incremental or continuous addition of a feed culture medium (e.g., liquid or solid culture medium) to an initial cell culture without substantial or significant removal of liquid culture medium from the cell culture. In some instances, the feed culture medium is the same as the first liquid culture medium present in the culture at the beginning of the culturing period. In other instances, the feed culture medium is a concentrated form of the first liquid culture medium and/or is added as a dry powder. In some examples of fed batch culture, two different feed culture media are added to the initial cell culture. “Specific productivity rate” or “SPR” as used herein refers to the mass or enzymatic activity of a recombinant protein produced per mammalian cell per day. The SPR for a recombinant antibody is usually measured as mass/cell/day. The SPR for a recombinant enzyme is usually measured as units/cell/day or (units/mass)/cell/day. “Volume productivity rate” or “VPR” as used herein refers to the mass or enzymatic activity of recombinant protein produced per volume of culture (e.g., per L of bioreactor, vessel, or tube volume) per day. The VPR for a recombinant antibody is usually measured as mass/L/day. The VPR for a recombinant enzyme is usually measured as units/L/day or mass/L/day. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Methods and materials are described herein for use in the present invention; other, suitable methods and materials known in the art can also be used. The materials, methods, and examples are illustrative only and not intended to be limiting. All publications, patent applications, patents, sequences, database entries, and other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. Other features and advantages of the invention will be apparent from the following detailed description and figures, and from the claims. DESCRIPTION OF DRAWINGS FIG. 1 is a graph showing the mean concentration (solid lines) of lactate over time in 10,000-L eculizumab production cell cultures (thin solid black lines) and previous 5-L eculizumab small scale cultures (thick solid black lines). The dashed lines represent ±1 standard deviation of the data. FIG. 2 is a graph showing the mean concentration (solid lines) of sodium over time in 10,000-L eculizumab production cell cultures (thin solid black lines) and previous 5-L eculizumab small scale cultures (thick solid black lines). The dashed lines represent ±1 standard deviation of the data. FIG. 3 is a graph showing the mean offline pH over time in 10,000-L eculizumab production cell cultures (thin solid black lines) and previous 5-L eculizumab small scale cultures (thick solid black lines). The dashed lines represent ±1 standard deviation of the data. FIG. 4 is a graph showing the mean viable cell density over time in 10,000-L eculizumab production cell cultures (thick solid black lines) and previous 5-L eculizumab small scale cultures (thin solid black lines). The dashed lines represent ±1 standard deviation of the data. FIG. 5 is a graph showing the mean viable cell density over time in 10,000-L eculizumab production cell cultures (thick solid black lines) and 5-L eculizumab small scale cultures cultured using the methods described herein (thin solid black lines) (n=40). The dashed lines represent ±1 standard deviation of the data. FIG. 6 is a graph showing the mean percentage cell viability over time in 10,000-L eculizumab production cell cultures (thick solid black lines) and previous 5-L eculizumab small scale cultures (thin solid black lines). The dashed lines represent ±1 standard deviation of the data. FIG. 7 is a graph showing the mean percentage cell viability over time in 10,000-L eculizumab production cell cultures (thick solid black lines) and 5-L eculizumab small scale cultures cultured using the methods described herein (thin solid black lines) (n=40). The dashed lines represent ±1 standard deviation of the data. FIG. 8 is a graph showing the titer of eculizumab over time in 10,000-L eculizumab production cell cultures (thick solid black lines) and previous 5-L eculizumab small scale cultures (thin solid black lines). The dashed lines represent ±1 standard deviation of the data. FIG. 9 is a graph showing the titer of eculizumab over time in 10,000-L eculizumab production cell cultures (thick solid black lines) and 5-L eculizumab small scale cultures cultured using the methods described herein (thin solid black lines) (n=40). The dashed lines represent ±1 standard deviation of the data. FIG. 10 is a graph showing the glucose concentration over time in 10,000-L eculizumab production cell cultures (thick solid black lines) and previous 5-L eculizumab small scale cultures (thin solid black lines). The dashed lines represent ±1 standard deviation of the data. FIG. 11 is a graph showing the glucose concentration over time in 10,000-L eculizumab production cell cultures (thick solid black lines) and 5-L eculizumab small scale cultures cultured during the methods described herein (thin solid black lines) (n=40). The dashed lines represent ±1 standard deviation of the data. DETAILED DESCRIPTION Provided herein are methods of methods of replicating a large-scale eculizumab production cell culture in a small scale culture. These methods include: providing a 2-L to 25-L bioreactor (such as a fed batch bioreactor) containing a first culture medium that occupies between about 60% and about 90% of the capacity of the bioreactor and has an initial cell density of between about 2.0×105 and about 7.5×105 NS0 cells/mL, the cells containing a recombinant eculizumab-encoding nucleic acid; culturing (such as fed batch culturing) the cells in the bioreactor for at least 8 days at about 34° C. to about 39° C., at a dO2 level of between about 10% to about 20%, and a rotary agitation of between about 40 RPM and about 80 RPM; maintaining (e.g., continuing) fed batch culturing until a cell density of about 10×105 to about 25×105 cells/mL is reached, then; (1) continuously adding a feed culture medium to the first culture medium for a period of about 100 hours to about 170 hours, and about 5 to 80 hours after the start of continuous addition of the feed culture medium, and (2) continuously adding an alkali base solution to the first culture medium for a period of between about 70 hours to about 105 hours. Non-limiting aspects of the methods of replicating a large-scale eculizumab production cell culture are described below. Any of the aspects described below can be used in any combination or with any other elements known in the art. Bioreactors A bioreactor used to culture the fed batch culture in any of the methods described herein can have a capacity of between about 2 L and about 25 L (e.g., between about 2 L and about 24 L, between about 2 L and about 22 L, between about 2 L and about 20 L, between about 2 L and about 18 L, between about 2 L and about 18 L, between about 2 L and about 16 L, between about 2 L and about 14 L, between about 2 L and about 12 L, between about 2 L and about 10 L, between about 2 L and about 9 L, between about 2 L and about 8 L, between about 2 L and about 7 L, between about 2 L and about 6 L, between about 2 L and about 5 L, between about 2 L and about 4 L, between about 2 L and about 3 L, between about 3 L and about 25 L, between about 3 L and about 24 L, between about 3 L and about 22 L, between about 3 L and about 20 L, between about 3 L and about 18 L, between about 3 L and about 16 L, between about 3 L and about 14 L, between about 3 L and about 12 L, between about 3 L and about 10 L, between about 3 L and about 9 L, between about 3 L and about 8 L, between about 3 L and about 7 L, between about 3 L and about 6 L, between about 3 L and about 5 L, between about 3 L and about 4 L, between about 4 L and about 25 L, between about 4 L and about 24 L, between about 4 L and about 22 L, between about 4 L and about 20 L, between about 4 L and about 18 L, between about 4 L and about 16 L, between about 4 L and about 14 L, between about 4 L and about 12 L, between about 4 L and about 10 L, between about 4 L and about 9 L, between about 4 L and about 8 L, between about 4 L and about 7 L, between about 4 L and about 6 L, between about 4 L and about 5 L, between about 5 L and about 25 L, between about 5 L and about 24 L, between about 5 L and about 22 L, between about 5 L and about 20 L, between about 5 L and about 18 L, between about 5 L and about 16 L, between about 5 L and about 14 L, between about 5 L and about 12 L, between about 5 L and about 10 L, between about 5 L and about 9 L, between about 5 L and about 8 L, between about 5 L and about 7 L, between about 5 L and about 6 L, between about 6 L and about 25 L, between about 6 L and about 24 L, between about 6 L and about 22 L, between about 6 L and about 20 L, between about 6 L and about 18 L, between about 6 L and about 16 L, between about 6 L and about 14 L, between about 6 L and about 12 L, between about 6 L and about 10 L, between about 6 L and about 9 L, between about 6 L and about 8 L, between about 6 L and about 7 L, between about 8 L and about 25 L, between about 8 L and about 24 L, between about 8 L and about 22 L, between about 8 L and about 20 L, between about 8 L and about 18 L, between about 8 L and about 16 L, between about 8 L and about 14 L, between about 8 L and about 12 L, between about 8 L and about 10 L, between about 8 L and about 9 L, between about 9 L and about 25 L, between about 9 L and about 24 L, between about 9 L and about 22 L, between about 9 L and about 20 L, between about 9 L and about 18 L, between about 9 L and about 16 L, between about 9 L and about 14 L, between about 9 L and about 12 L, between about 9 L and about 10 L, between about 10 L and about 25 L, between about 10 L and about 24 L, between about 10 L and about 22 L, between about 10 L and about 20 L, between about 10 L and about 18 L, between about 10 L and about 16 L, between about 10 L and about 14 L, between about 10 L and about 12 L, between about 12 L and about 25 L, between about 12 L and about 24 L, between about 12 L and about 22 L, between about 12 L and about 20 L, between about 12 L and about 18 L, between about 12 L and about 16 L, between about 12 L and about 14 L, between about 14 L and about 25 L, between about 14 L and about 24 L, between about 14 L and about 22 L, between about 14 L and about 20 L, between about 14 L and about 18 L, between about 14 L and about 16 L, between about 16 L and about 25 L, between about 16 L and about 24 L, between about 16 L and about 22 L, between about 16 L and about 20 L, between about 16 L and about 18 L, between about 18 L and about 25 L, between about 18 L and about 24 L, between about 18 L and about 22 L, between about 18 L and about 20 L, between about 20 L and about 25 L, between about 20 L and about 24 L, between about 20 L and about 22 L, or between about 22 L and about 25 L). First Culture Medium The methods provided herein include a step of providing a bioreactor containing a first culture medium (e.g., any of the exemplary types of culture media described herein or known in the art). The volume of the first culture medium present in the bioreactor can be between about 60% and about 90% (e.g., between about 60% and about 88%, between about 60% and about 86%, between about 60% and about 86%, between about 60% and about 84%, between about 60% and about 82%, between about 60% and about 80%, between about 60% and about 78%, between about 60% and about 76%, between about 60% and about 74%, between about 60% and about 72%, between about 60% and about 70%, between about 60% and about 68%, between about 60% and about 66%, between about 60% and about 64%, between about 62% and about 90%, between about 62% and about 88%, between about 62% and about 86%, between about 62% and about 84%, between about 62% and about 82%, between about 62% and about 80%, between about 62% and about 78%, between about 62% and about 76%, between about 62% and about 74%, between about 62% and about 72%, between about 62% and about 74%, between about 62% and about 72%, between about 62% and about 70%, between about 62% and about 68%, between about 62% and about 66%, between about 64% and about 90%, between about 64% and about 88%, between about 64% and about 86%, between about 64% and about 84%, between about 64% and about 82%, between about 64% and about 80%, between about 64% and about 78%, between about 64% and about 76%, between about 64% and about 74%, between about 64% and about 72%, between about 64% and about 70%, between about 64% and about 68%, between about 68% and about 90%, between about 68% and about 88%, between about 68% and about 86%, between about 68% and about 84%, between about 68% and about 82%, between about 68% and about 80%, between about 68% and about 78%, between about 68% and about 76%, between about 68% and about 74%, between about 68% and about 72%, between about 70% and about 90%, between about 70% and about 88%, between about 70% and about 86%, between about 70% and about 84%, between about 70% and about 82%, between about 70% and about 80%, between about 70% and about 78%, between about 70% and about 76%, between about 70% and about 74%, between about 72% and about 90%, between about 72% and about 88%, between about 72% and about 86%, between about 72% and about 84%, between about 72% and about 82%, between about 72% and about 80%, between about 72% and about 78%, between about 72% and about 76%, between about 74% and about 90%, between about 74% and about 88%, between about 74% and about 86%, between about 74% and about 82%, between about 74% and about 80%, between about 74% and about 78%, between about 76% and about 90%, between about 78% and about 90%, between about 78% and about 88%, between about 78% and about 86%, between about 78% and about 84%, between about 78% and about 82%, between about 80% and about 90%, between about 80% and about 88%, between about 80% and about 86%, between about 80% and about 84%, between about 82% and about 90%, between about 82% and about 88%, between about 82% and about 86%, between about 84% and about 90%, between about 84% and about 88%, or between about 86% and about 90%) of the capacity of the bioreactor. The volume of the first culture medium present in the bioreactor can be between about 1.2 L and about 22 L (e.g., between about 1.2 L and about 20 L, between about 1.2 L and about 18 L, between about 1.2 L and about 16 L, between about 1.2 and about 14 L, between about 1.2 and about 12 L, between about 1.2 L and about 10 L, between about 1.2 L and about 9 L, between about 1.2 L and about 8 L, between about 1.2 L and about 7 L, between about 1.2 L and about 6 L, between about 1.2 L and about 5 L, between about 1.2 L and about 4 L, between about 1.2 L and about 3 L, between about 1.2 L and about 2 L, between about 2.0 L and about 22 L, between about 2.0 L and about 20 L, between about 2 L and about 18 L, between about 2 L and about 16 L, between about 2 L and about 14 L, between about 2 L and about 12 L, between about 2 L and about 10 L, between about 2 L and about 9 L, between about 2 L and about 8 L, between about 2 L and about 7 L, between about 2 L and about 6 L, between about 2 L and about 5 L, between about 2 L and about 4 L, between about 2 L and about 3 L, between about 3 L and about 22 L, between about 3 L and about 20 L, between about 3 L and about 18 L, between about 3 L and about 16 L, between about 3 L and about 14 L, between about 3 L and about 12 L, between about 3 L and about 10 L, between about 3 L and about 9 L, between about 3 L and about 8 L, between about 3 L and about 7 L, between about 3 L and about 6 L, between about 3 L and about 5 L, between about 3 L and about 4 L, between about 4 L and about 22 L, between about 4 L and about 20 L, between about 4 L and about 18 L, between about 4 L and about 16 L, between about 4 L and about 14 L, between about 4 L and about 12 L, between about 4 L and about 10 L, between about 4 L and about 9 L, between about 4 L and about 8 L, between about 4 L and about 7 L, between about 4 L and about 6 L, between about 4 L and about 5 L, between about 5 L and about 22 L, between about 5 L and about 20 L, between about 5 L and about 18 L, between about 5 L and about 16 L, between about 5 L and about 14 L, between about 5 L and about 12 L, between about 5 L and about 10 L, between about 5 L and about 9 L, between about 5 L and about 8 L, between about 5 L and about 7 L, between about 5 L and about 6 L, between about 6 L and about 22 L, between about 6 L and about 20 L, between about 6 L and about 18 L, between about 6 L and about 16 L, between about 6 L and about 14 L, between about 6 L and about 12 L, between about 6 L and about 10 L, between about 6 L and about 9 L, between about 6 L and about 8 L, between about 6 L and about 7 L, between about 7 L and about 22 L, between about 7 L and about 20 L, between about 7 L and about 18 L, between about 7 L and about 16 L, between about 7 L and about 14 L, between about 7 L and about 12 L, between about 7 L and about 10 L, between about 7 L and about 9 L, between about 7 L and about 8 L, between about 8 L and about 22 L, between about 8 L and about 20 L, between about 8 L and about 18 L, between about 8 L and about 16 L, between about 8 L and about 14 L, between about 8 L and about 12 L, between about 8 L and about 10 L, between about 8 L and about 9 L, between about 9 L and about 22 L, between about 9 L and about 20 L, between about 9 L and about 18 L, between about 9 L and about 16 L, between about 9 L and about 14 L, between about 9 L and about 12 L, between about 9 L and about 10 L, between about 10 L and about 22 L, between about 10 L and about 20 L, between about 10 L and about 18 L, between about 10 L and about 16 L, between about 10 L and about 14 L, between about 10 L and about 12 L, between about 12 L and about 22 L, between about 12 L and about 20 L, between about 12 L and about 18 L, between about 12 L and about 16 L, between about 12 L and about 14 L, between about 14 L and about 22 L, between about 14 L and about 20 L, between about 14 L and about 18 L, between about 14 L and about 16 L, between about 16 L and about 22 L, between about 16 L and about 20 L, between about 16 L and about 18 L, between about 18 L and about 22 L, between about 18 L and about 20 L, or between about 20 L and about 22 L). NS0 Cells and Initial Cell Density The NS0 cells used in the methods described herein can contain a recombinant nucleic acid that is stably integrated in the NS0 cell's genome and encodes a recombinant eculizumab. In some embodiments, the recombinant eculizumab is secreted by the mammalian cell into the liquid culture medium. In some instances, the cultured mammalian cells are derived from a seed culture. More particularly, the initial cell culture is the result of a seed train process or a culture from another bioreactor. In non-limiting examples of any of the methods described herein, the cell density of mammalian cells present in the bioreactor at the start of the fed batch culturing period (initial cell density, such as initial viable cell density) is about 1.0×105 cells/mL to about 7.5×105 cells/mL, about 2.0×105 cells/mL to about 7.5×105 cells/mL, about 3.0×105 cells/mL to about 7.5×105 cells/mL, between about 4.0×105 cells/mL to about 7.5×105 cells/mL, between about 5.0×105 cells/mL to about 7.5×105 cells/mL, between about 6.0×105 cells/mL to about 7.5×105 cells/mL, between about 7.0×105 cells/mL to about 7.5×106 cells/mL, between about 1.0×105 cells/mL to about 7.0×105 cells/mL, between about 2.0×105 cells/mL to about 7.0×105 cells/mL, between about 3.0×105 cells/mL to about 7.0×105 cells/mL, between about 4.0×105 cells/mL to about 7.0×105 cells/mL, between about 5.0×105 cells/mL to about 7.0×105 cells/mL, between about 6.0×105 cells/mL to about 7.0×105 cells/mL, between about 1.0×105 cells/mL to about 6.0×105 cells/mL, between about 2.0×105 cells/mL to about 6.0×105 cells/mL, between about 3.0×105 to about 6.0×105 cells/mL, between about 4.0×105 cells/mL to about 6.0×105 cells/mL, between about 5.0×105 cells/mL to about 6.0×105 cells/mL, between about 1.0×105 cells/mL to about 5.0×105 cells/mL, between about 2.0×105 cells/mL to about 5.0×105 cells/mL, between about 3.0×105 cells/mL to about 5.0×105 cells/mL, between about 4.0×105 cells/mL to about 5.0×105 cells/mL, between about 1.0×105 cells/mL to about 4.0×105 cells/mL, between about 2.0×105 cells/mL to about 4.0×105 cells/mL, between about 3.0×105 cells/mL to about 4.0×105 cells/mL, between about 1.0×105 cells/mL to about 3.0×105 cells/mL, between about 2.0×105 cells/mL to about 3.0×105 cells/mL, or between about 1.0×105 cells/mL to about 2.0×105 cells/mL. Fed Batch Culturing As is known in the art, fed batch culturing includes the incremental (periodic) or continuous addition of a feed culture medium to an initial cell culture without substantial or significant removal of the first culture medium from the cell culture. The cell culture in fed batch culturing can be disposed in a bioreactor (e.g., any of the exemplary bioreactors described herein). In some instances, the feed culture medium is the same as the first culture medium. The feed culture medium may be either in a liquid form or a dry powder. In some examples instances, the feed culture medium is a concentrated form of the first culture medium. For example, the addition of the feed culture medium (continuously or periodically) can occur at a time point that is between 6 hours and 7 days, between about 6 hours and about 6 days, between about 6 hours and about 5 days, between about 6 hours and about 4 days, between about 6 hours and about 3 days, between about 6 hours and about 2 days, between about 6 hours and about 1 day, between about 12 hours and about 7 days, between about 12 hours and about 6 days, between about 12 hours and about 5 days, between about 12 hours and about 4 days, between about 12 hours and about 3 days, between about 12 hours and about 2 days, between about 1 day and about 7 days, between about 1 day and about 6 days, between about 1 day and about 5 days, between about 1 day and about 4 days, between about 1 day and about 3 days, between about 1 day and about 2 days, between about 2 days and about 7 days, between about 2 days and about 6 days, between about 2 days and about 5 days, between about 2 days and about 4 days, between about 2 days and about 3 days, between about 3 days and about 7 days, between about 3 days and about 6 days, between about 3 days and about 5 days, between about 3 days and about 4 days, between about 4 days and about 7 days, between about 4 days and about 6 days, between about 4 days and about 5 days, between about 5 days and about 7 days, or between about 5 days and about 6 days, after the start of the culturing period. In some examples, two different feed culture media (e.g., any of the exemplary liquid culture media described herein) are added (continuously or incrementally) during feed batch culturing. The amount or volume of the first feed culture medium and the second feed culture medium added can be substantially the same or can differ. The first feed culture medium can be in the form of a liquid and the second feed culture medium can be in the form of a solid. In some examples, the first and the second feed culture media are liquid culture media. The fed batch culturing can be performed for at least 8 days (e.g., 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, or 25 days). For example, the fed batch culturing can be performed for at least 9 days, at least 10 days, at least 11 days, at least 12 days, at least 13 days, at least 14 days, at least 15 days, at least 16 days, at least 17 days, at least 18 days, at least 19 days, or at least 20 days. The feed culture medium (or first and second liquid feed culture media) can be added continuously or periodically. Continuous Addition of a Feed Culture Medium When the feed culture medium is added continuously, the rate of addition of the feed culture medium can be held constant or can be increased (e.g., steadily increased) over the culturing period. A continuous addition of feed culture medium can start at a specific time point during the culturing period (e.g., when the cells reach a target cell density, e.g., any of the exemplary target cell densities described herein). In some embodiments, the continuous addition of feed culture medium can be initiated at day 2, day 3, day 4, day 5, day 6, or day 7 of the culturing period. Exemplary volumes and rates of adding a feed culture medium (e.g., a first and second liquid feed culture media) to a culture are described herein. In some embodiments a single liquid feed culture medium or a first and second liquid feed culture media are added continuously to the cell culture. The rate of addition of the single liquid feed culture medium or the rate of addition of each of the first and the second liquid feed culture medium can be between about 10 μL/minute and about 160 μL/minute (e.g., between about 10 μL/minute and about 150 μL/minute, between about 10 μL/minute and about 140 μL/minute, between about 10 μL/minute and about 130 μL/minute, between about 10 μL/minute and about 120 μL/minute, between about 10 μL/minute and about 110 μL/minute, between about 10 μL/minute and about 100 μL/minute, between about 10 μL/minute and about 90 μL/minute, between about 10 μL/minute and about 80 μL/minute, between about 10 μL/minute and about 75 μL/minute, between about 10 μL/minute and about 70 μL/minute, between about 10 μL/minute and about 65 μL/minute, between about 10 μL/minute and about 60 μL/minute, between about 10 μL/minute and about 55 μL/minute, between about 10 μL/minute and about 50 μL/minute, between about 10 μL/minute and about 45 μL/minute, between about 10 μL/minute and about 40 μL/minute, between about 10 μL/minute and about 35 μL/minute, between about 10 μL/minute and about 30 μL/minute, between about 10 μL/minute and about 25 μL/minute, between about 10 μL/minute and about 20 μL/minute, between about 10 μL/minute and about 15 μL/minute, between about 15 μL/minute and about 160 μL/minute, 15 μL/minute and about 150 μL/minute, between about 15 μL/minute and about 140 μL/minute, between about 15 μL/minute and about 130 μL/minute, between about 15 μL/minute and about 120 μL/minute, between about 15 μL/minute and about 110 μL/minute, between about 15 μL/minute and about 100 μL/minute, between about 15 μL/minute and about 90 μL/minute, between about 15 μL/minute and about 80 μL/minute, between about 15 μL/minute and about 75 μL/minute, between about 15 μL/minute and about 70 μL/minute, between about 15 μL/minute and about 65 μL/minute, between about 15 μL/minute and about 60 μL/minute, between about 15 μL/minute and about 55 μL/minute, between about 15 μL/minute and about 50 μL/minute, between about 15 μL/minute and about 45 μL/minute, between about 15 μL/minute and about 40 μL/minute, between about 15 μL/minute and about 35 μL/minute, between about 15 μL/minute and about 30 μL/minute, between about 15 μL/minute and about 25 μL/minute, between about 15 μL/minute and about 20 μL/minute, between about 20 μL/minute and about 160 μL/minute, between about 20 μL/minute and about 150 μL/minute, between about 20 μL/minute and about 140 μL/minute, between about 20 μL/minute and about 130 μL/minute, between about 20 μL/minute and about 120 μL/minute, between about 20 μL/minute and about 110 μL/minute, between about 20 μL/minute and about 100 μL/minute, between about 20 μL/minute and about 90 μL/minute, between about 20 μL/minute and about 80 μL/minute, between about 20 μL/minute and about 75 μL/minute, between about 20 μL/minute and about 70 μL/minute, between about 20 μL/minute and about 65 μL/minute, between about 20 μL/minute and about 60 μL/minute, between about 20 μL/minute and about 55 μL/minute, between about 20 μL/minute and about 50 μL/minute, between about 20 μL/minute and about 45 μL/minute, between about 20 μL/minute and about 40 μL/minute, between about 20 μL/minute and about 35 μL/minute, between about 20 μL/minute and about 30 μL/minute, between about 20 μL/minute and about 25 μL/minute, between about 30 μL/minute and about 160 μL/minute, 30 μL/minute and about 150 μL/minute, between about 30 μL/minute and about 140 μL/minute, between about 30 μL/minute and about 130 μL/minute, between about 30 μL/minute and about 120 μL/minute, between about 30 μL/minute and about 110 μL/minute, between about 30 μL/minute and about 100 μL/minute, between about 30 μL/minute and about 90 μL/minute, between about 30 μL/minute and about 80 μL/minute, between about 30 μL/minute and about 70 μL/minute, between about 30 μL/minute and about 60 μL/minute, between about 30 μL/minute and about 50 μL/minute, between about 30 μL/minute and about 40 μL/minute, between about 40 μL/minute and about 160 μL/minute, between about 40 μL/minute and about 150 μL/minute, between about 40 μL/minute and about 140 μL/minute, between about 40 μL/minute and about 130 μL/minute, between about 40 μL/minute and about 120 μL/minute, between about 40 μL/minute and about 110 μL/minute, between about 40 μL/minute and about 100 μL/minute, between about 40 μL/minute and about 90 μL/minute, between about 40 μL/minute and about 80 μL/minute, between about 40 μL/minute and about 70 μL/minute, between about 40 μL/minute and about 60 μL/minute, between about 40 μL/minute and about 50 μL/minute, between about 50 μL/minute and about 160 μL/minute, between about 50 μL/minute and about 150 μL/minute, between about 50 μL/minute and about 140 μL/minute, between about 50 μL/minute and about 130 μL/minute, between about 50 μL/minute and about 120 μL/minute, between about 50 μL/minute and about 110 μL/minute, between about 50 μL/minute and about 100 μL/minute, between about 50 μL/minute and about 90 μL/minute, between about 50 μL/minute and about 80 μL/minute, between about 50 μL/minute and about 70 μL/minute, between about 50 μL/minute and about 60 μL/minute, between about 60 μL/minute and about 160 μL/minute, between about 60 μL/minute and about 150 μL/minute, between about 60 μL/minute and about 140 μL/minute, between about 60 μL/minute and about 130 μL/minute, between about 60 μL/minute and about 120 μL/minute, between about 60 μL/minute and about 110 μL/minute, between about 60 μL/minute and about 100 μL/minute, between about 60 μL/minute and about 90 μL/minute, between about 60 μL/minute and about 80 μL/minute, between about 60 μL/minute and about 70 μL/minute, between about 70 μL/minute and about 160 μL/minute, between about 70 μL/minute and about 150 μL/minute, between about 70 μL/minute and about 140 μL/minute, between about 70 μL/minute and about 130 μL/minute, between about 70 μL/minute and about 120 μL/minute, between about 70 μL/minute and about 110 μL/minute, between about 70 μL/minute and about 100 μL/minute, between about 70 μL/minute and about 90 μL/minute, between about 70 μL/minute and about 80 μL/minute, between about 80 μL/minute and about 160 μL/minute, between about 80 μL/minute and about 150 μL/minute, between about 80 μL/minute and about 140 μL/minute, between about 80 μL/minute and about 130 μL/minute, between about 80 μL/minute and about 120 μL/minute, between about 80 μL/minute and about 110 μL/minute, between about 80 μL/minute and about 100 μL/minute, between about 80 μL/minute and about 90 μL/minute, between about 90 μL/minute and about 160 μL/minute, between about 90 μL/minute and about 150 μL/minute, between about 90 μL/minute and about 140 μL/minute, between about 90 μL/minute and about 130 μL/minute, between about 90 μL/minute and about 120 μL/minute, between about 90 μL/minute and about 110 μL/minute, between about 90 μL/minute and about 100 μL/minute, between about 100 μL/minute and about 160 μL/minute, between about 100 μL/minute and about 140 μL/minute, between about 100 μL/minute and about 120 μL/minute, between about 120 μL/minute and about 160 μL/minute, between about 120 μL/minute and about 140 μL/minute, or between
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