Ataxin-1 regulates epithelial-mesenchymal transition of cervical cancer cells

SUPPLEMENTARY METHODS

For
wound-healing assays, HeLa shATXN1-#1 , HeLa shATXN1-#2 , and control cells were allowed to adhere to the surfaces of six-well plates. After a pretreatment with mitomycin C (10 μg/ml) in DMEM for 2 h, 10-μl pipette tips were used to introduce longitudinal straight scratches in the cell layers. After wounding, plates were imaged using a Zeiss inverted microscope equipped with a 5× phase-contrast objective. Representative wound healing images were taken at the time of the scratch and 0-72 h after the wound scratch in stable HeLa cells.
Supplementary Figure 1:
The migration of HeLa shATXN1-#1 , HeLa shATXN1-#2 , and control cells was assayed in a woundhealing assay. * P<0.05, ** P<0.01, *** P<0.001, t test. Scale bar: 100 μm.
SUPPLEMENTARY FIGURE


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