Abstract
Human herpes viruses are responsible for the several transmitted infections in human. It is known that the DNA polymerase enzyme is one of the putative targets for herpes. Therefore, it is of interest to model all known DNA polymerases of Herpesviridae family. Here, all the DNA polymerases of Herpesviridae without any crystal structure were modeled using HHV-1 DNA polymerase as a template. Modeled structures were screened by ramachandran plot and Descrete Optimization of Protein Energy (DOPE) score. To find out multi-target inhibitor for Herpesviridae, 21 natural antiviral compounds were selected from literature and screened using Lipinski's rule of five. Binding pose of acyclovir with HHV-1 DNA polymerase was taken for the comparative docking study. Comparative binding analysis was done after settling of 120 and eight partial mono flexible protein-ligand docking sets for natural compounds and acyclovir, respectively. From the study it is found that alliin and gallic acid exhibit good binding affinity than acyclovir and other natural compounds. So, here we purpose that these two compounds can be potential candidates to inhibit Herpesviridae family.
INTRODUCTION
Herpes viruses are the leading cause of several transmitted infections in humans that are chronic, widespread and infectious during both its symptomatic and asymptomatic periods and are second only to influenza and cold viruses (Bernardino et al., 2008; Smith and Kennell, 1981) . Infections caused by this family of viruses are endemic and sexual contact is a significant mode of transmission for herpes simplex virus 1 and 2 (HSV-1, HSV-2), human cytomegalovirus (HHV-5) and likely Karposi's sarcoma herpesvirus (HHV-8) (Anderson et al., 2014; Collins and Medveczky, 2002) . The implication of Epstein-Barr viruses (HHV-4), Varicella Zoster Viruses (HHV-3) and Roseoloviruses (HHV-6 and HHV-7) as cofactors in human cancers and several skin infections create urgency for a better understanding of this complex (Morissette and Flamand, 2010; Yamamoto and Nakamura, 2000; Karatas et al., 2008) . The drug target in structure-based drug design is mostly proteins that belong to the families of glycoprotein and DNA polymerase (Hudnall et al., 2004) . A common therapy for herpes viral infections employs nucleoside analogs, such as acyclovir and valacyclovir which targets the viral DNA polymerase, essential for viral DNA replication (Prepens et al., 2007) . This enzyme is an attractive target for the development of antiviral drugs, since the human herpes viruses share a common replication cycle in which the viral DNA polymerase acts in several points, being extremely important after the start of the beta-phase of viral replication cycle (VilibicCavlek et al., 2011) .
INTRODUCTION
Although a number of clinically useful drugs are available in the market for the treatment of human herpesvirus (HHV) infections but still there is no effective therapy for all the HHVs. The major limitations of the currently available antiherpetic drug therapy are drug resistance & host toxicity (Gupta and Wald, 2006) . Moreover, the narrow antiviral spectrum and resistance of DNA polymerases of Herpesviridae family, to these agents is an emerging problem for the disease management (Gottlieb et al., 2014) . A better understanding of the target structures (DNA Polymerases) of Herpesviridae family will help to understand the development of new safe and effective broad spectrum antiherpetic drugs that fill an unmet need. In the present study, DNA polymerases of Herpesviridae family were modeled using homology modeling approach. The parameters used for the screening of the structure models were DOPE score and ramachandran (RC)plot analysis. Each model was processed through energy minimization step to achieve the accuracy and stability of the generated structures. A set of 21 compounds (which are known for their antiviral activity) were taken and initially screened by lipinski's rule of five. Fifteen screened compounds were further docked at the active site of the DNA polymerases of Herpesviridae family family. From the set of screened 15 compounds, alliin and gallic acid showed good binding affinity than the control acyclovir. These findings may provide useful insights for designing new potent multi-target inhibitors to fight against the infections of Herpesviridae family.
MATERIALS AND METHODOLOGY

Sequence & Phylogenetic Analysis
The amino acid sequences of all the DNA polymerases of Herpesviridae family were deduced from the NCBI database (http://www.ncbi.nlm.nih.gov/protein). The multiple sequence alignment was carried out using ClustalX 2.1 for the identification of conserved residues throughout the Herpesviridae family. For further analysis among the Herpesviridae family, a phylogenetic tree was generated using maximum likelihood (ML) method of sea-view program. Bootstrap support was calculated from 100 replicates. To identify the suitable template in the Protein Data Bank (PDB) for the modeling of the target sequences, BLASTp was used with the default parameters.
Homology Modeling and Screening of protein models
Modeller9.9 was used to model DNA polymerases of Herpesviridae family by using HHV-1 DNA polymerase (PDB ID: kaiserdebra@example.org Å resolution) as template (Sali et al., 1995) . Screening of ten generated models for each target was done with DOPE score and RC plot analysis. DOPE score is a reference state that corresponds to non-interacting atoms in a homogeneous sphere with the radius dependent on a sample original structure and can be computed using Modeller9.9 (Shenand Sali, 2006) . PROCHECK was used to obtain RC plots to evaluate the backbone conformation by inspecting the Psi/Phi dihedral angles (Laskowski et al., 1993) . The validation is done on the basis of various parameters such as lengths, angles and planarity of the peptide bonds, hydrogen bond geometry.
Energy Minimization
Gromacs 4.6.7 software package was used for the energy minimization studies of all seven screened models using Gromos53a6 force field. Pdb2gmx command was used to generate the topology of each model. An octahedron box was used for the solvation requirements with the distance of 1.2 Å between solute and the box. Simple Point Charge Extended (SPC/E) water model was used in order to solvate the system. Sodium and Chloride ions were used to neutralize the system by replacing water molecules from the system. Finally, system was processed through energy minimization step using steepest-descent algorithm for 50,000 steps without any restraints. Periodic Boundary Conditions were used with rlist value of 0.9 nm, rcoulomb 0.9 nm and rvdw value of 1.4 nm.
Ligand Selection
In order to find the potent ligand molecules for Herpesviridae family, a set of 21 natural compounds (known antiviral activity), was taken from the literature. Lipinski's rule of five was used as the first filter to screen the drug-like compounds. The molecular structures were drawn by the Chemsketch (Li et al., 2004) and molecules were converted into protein data bank (.pdb) format from MDL (.mol) by using OpenBabel (O'Boyle et al., 2011) .
Molecular Docking & Visualization
Molecular docking of all the screened compounds was carried out using Autodock4.2 software package from The Scripps Research Institute (Morris et al.,2009) . To get the docking interactions close to the experimental analysis, partial mono rigidflexible docking was used wherein target is kept rigid and binding site residues were allowed to move with flexible ligand molecule (Gupta et al.,2015) . Initially, all the receptors and ligands are made to undergo a pre-optimization process using the autodock parameters like atom types, torsion modes and partial charges. A grid box of the size 42×52×42 points covering the binding site residues was used throughout the docking analysis. A grid spacing of 0.450 Å was used to ensure the standard grid spacing around the binding site.
Molecular Docking & Visualization
Lamarckian Genetic Algorithm (LGA) was applied to find out the most favorable interactions. During each docking experiment, 100 runs were carried out with ten experiments for each ligand by keeping default parameters. The confirmation with most favorable free energy of binding and lowest root mean square deviation (RMSD) was selected. Visualization of the docked structure was performed on PyMol molecular graphics program, a comprehensive software package for rendering and animating 3D-structures (Lilland Danielson, 2011) .
RESULTS AND DISCUSSIONS
Binding mode of acyclovir and HHV-1 DNA polymerase has already been reported that explains acyclovir interacts with highly conserved KKKY motif present in the binding cleft to block the replication of viral DNA. Studies also state that the resistance to nucleoside inhibitors is a result of mutations in highly conserved region YGDTDS of HHV-1 DNA polymerase. The amino acid residues of YGDTDS region do not play any role in the catalysis but affects the binding cleft leading to the rejection of the nucleoside inhibitors. So, during the interaction analysis of any inhibitor it is very important to study both these signature elements of the HHV-1 DNA polymerase (Shenping et al., 2006; Baltz et al., 2009; Terrell and Coen, 2012) . The DNA polymerase sequences of all eight members of Herpesviridae family were aligned to find the homology (Figure  1) . Interestingly, YGDTDS region was found highly conserved throughout the virus family, wherein KKKY motif, a lysine residue was found replaced by an arginine residue from KKKY motif. Transformation of the motif KKKY to KKRY explains the specificity of the positively charged binding pocket for the catalysis. Phylogenetic analysis of DNA polymerases of Herpesviridae family clearly follows the natural evolutionary classification which states that HHV-1, HHV-2 and HHV-3 are in alpha subfamily; HHV-5, HHV-6 and HHV-7 are in beta subfamily, whereas HHV-4 and HHV-8 are in gamma subfamily (Figure 2) (Gable et al., 2014) . By homology modeling, ten models were generated for all the DNA polymerases of Herpesviridae family and the best models were selected on the basis of ramachandran plot analysis (High Core region and low disallowed region) and DOPE score analysis (low energy value) (Table 1) . To increase the reliability of all the screened models, energy minimization was carried out in the presence of water molecules.
RESULTS AND DISCUSSIONS
The focus of the present study is also to elucidate the action mechanism of naturally occurring antiviral compounds in inhibiting viral proteins of Herpesviridae family, as there is still a lack of clinically potential inhibitors. Hence, it would be a promising choice to block the Herpesviridae family with suitable inhibitors. Consequently, we have prepared a set of 21 natural antiviral compounds and screened on the basis of Lipinski's rule of five (Supplementary Table 1) .
RESULTS AND DISCUSSIONS
Virtual screening methods are routinely hand extensively used to reduce the cost and time of drug discovery process. The analysis of Lipinski's rule of five showed that nimbin, gallocatechin, chicoric acid, diosgenin, taraxerol and caryophyllene violates the rule. So these compounds were eliminated from docking process (Table 2) . Acyclovir was considered as a control and docked with all eight DNA polymerases of the Herpesviridae family. For the 15 best screened compounds, 120 sets of partial mono flexible Protein-Ligand docking was carried outwith all eight DNA polymerase targets (Supplementary Tables 2 to 9 ). The binding pose of acyclovir at the active site of HHV-1 DNA polymerase was used to analyze the (Figure 3) . From the docking results, it was observed that alliin (Molecule 4) and gallic acid (Molecule 14) have good binding affinity compared to acyclovir and other natural antiviral compounds (Table 3) . Both the compounds also qualify the Lipinski's filter as well as possess good molecular properties of drug likeness. Both the compounds were also found closely interacting with catalytic residues and well fitted into the binding cavity to generate stable complex (Figure 4 & 5) .
CONCLUSION
Recent studies showed increasing incidents of infections by Herpesviridae family, hence, there is a need to control Herpesviridae infection using suitable multi target inhibitors. For this, a better understanding of the structure of the target proteins is needed. So, here we documented the structure model of DNA polymerases of Herpesviridae family members (apart from HHV-1 DNA polymerase). Binding poses of acyclovir with the DNA polymerases of Herpesviridae family were determined using partial rigid-flexible docking. The docking studies also contributed in the identification of alliin and gallic acid with good binding affinity to DNA polymerases of the Herpesviridae family in comparison to other natural compounds as well as their substrate acyclovir. So from all these observations we propose that alliin and gallic acid may block the Herpesviridae family and provide a significant basis for the antiherpetic drug development. However, in vitro and experimental evaluation is needed to consider these molecules as a suitable drug against the Herpesviridae family. 

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