 during these strange, extraordinary times.
In Austin, Food & Wine named Nixta Taqueria among these notable restaurants. The nondescript East Austin restaurant, tucked away on East 12th Street in Rosewood, opened in October 2019 and has been one of the city’s liveliest hotspots ever since.
The magazine calls co-owners Edgar Rico and Sara Mardanbigi’s decision to open a taco spot in Austin “brave” and praises Chef Rico’s homemade tortillas, made by grinding heirloom corn in-house. “I would pay a very large sum of money to spend another afternoon breaking through a plate of duck confit tacos at Nixta Taqueria in Austin,” writes Shah.
Nixta also had some pandemic-related issues. Just months after its successful launch, COVID-19 hit the city, causing the couple to focus on a take-away model.
Nixta joins nine other US restaurants, including Los Angeles, Birmingham and New Orleans. It was the only Texan restaurant that made the list.
<issue_start><issue_comment>Title: Remove misleading ProcessTipsetResponse which implied that errors are not ignored.
username_0: ### Motivation
The `ProcessTipsetResponse` contained fields which indicated which messages failed, but these were ignored by callers.

### Proposed changes
Removed the custom response type in favour of returning a slice of the message results (pass thru from ApplyMessagesEtc) and document the dropping of errors.

I suggest this land before #3622, and that that PR must now handle these errors somehow.
<issue_comment>username_0: This appears to have broken all the daemon tests that use messagewait. It might take me a while to figure out why - please be on the lookout for dumdums.
<issue_comment>username_0: I've rebased against #3622 and it's much simpler now.Because it's considered protocol to disrobe before receiving a thorough pomping. We as a nation of dumb-fuck voter fodder (one must consider the entire populous, not just the exceptionally enlightened who are fortunate enough to be reading this) have obviously been prancing around in a very suggestively short skirt and gaggin' for it.
I should have been hired to write the speech, although I fear that "Mr Push My Spectacles Up With My Middle Finger In A Thinly Veiled Fuck You To The Masses" would struggle with some of the bigger whedds.
I'm sure the opportunistic bastards currently sweating all over the seats of Parliament are quite capable of jobs or portfolios to which they have been appointed. I mean, look at the splendid endeavour they apply to enriching themselves with their "Let them eat cake" attitude. And getting away with it! You have to take your hat off to a group of people with the con skills to keep the masses happy on "we don't have anything, we're just grateful that we have the right to have something"...
Public service? Public service be damned! Public service is for people who care more about their constituents than their personal wealth or power. The last true high profile public servant was Mother Theresa. By definition a politician is "one who displays more cunning, artifice and despicably devious design to leech votes from the downtrodden proletariat."
So. To my version of today's much anticipated speech...
"Oh Hai! Is this thing on?*
Madam Speaker, Ministers, Ladies and Gentlemen, and The Nation.*
You're fucked. We're in bed with China and they hold the purse strings.* We didn't think that out very well, but a few job losses do nothing to deter us from our Africanisation of this Rainbow Nation.* It is all-inclusive.* Even the previously advantaged are fucked.*
I am glad to announce that we have managed to think of ways to fudge the crime statistics to show only marginal increases and bring in unsuspecting tourists that you can rob* - this will from now on be called 'Job Creation'.*
Going forward,* nothing will change no matter how clever Zapiro thinks he is getting with his counter-revolutionary cartoons.*
Goodnight* and thank you for blockading every single road in and out of Cape Town for 2 days, when I could just as easily have done this via Skype, Oprah style.*"
*[Pause for effect, the effect being that I can't read this shit in front of me. Give nationwide television audience the finger...]
And that, folks, is that. Anything else is an utter fabrication.
NGDG: "If I want your advice, I'll Google it."
Spread The Love. Your Nation Needs You.Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production

Background Deletion of large blocks of nonessential genes that are not needed for metabolic pathways of interest can reduce the production of unwanted by-products, increase genome stability, and streamline metabolism without physiological compromise. Researchers have recently constructed a reduced-genome Escherichia coli strain MDS42 that lacks 14.3% of its chromosome. Results Here we describe the reengineering of the MDS42 genome to increase the production of the essential amino acid L-threonine. To this end, we over-expressed a feedback-resistant threonine operon (thrA*BC), deleted the genes that encode threonine dehydrogenase (tdh) and threonine transporters (tdcC and sstT), and introduced a mutant threonine exporter (rhtA23) in MDS42. The resulting strain, MDS-205, shows an ~83% increase in L-threonine production when cells are grown by flask fermentation, compared to a wild-type E. coli strain MG1655 engineered with the same threonine-specific modifications described above. And transcriptional analysis revealed the effect of the deletion of non-essential genes on the central metabolism and threonine pathways in MDS-205. Conclusion This result demonstrates that the elimination of genes unnecessary for cell growth can increase the productivity of an industrial strain, most likely by reducing the metabolic burden and improving the metabolic efficiency of cells.


Background
The vast increase in annotated genome information and high-throughput technologies has enabled a systematic improvement of industrial microbes through genome engineering. Restructuring of microbial genomes has been shown to have several advantages over conventional approaches for strain improvement [1][2][3][4][5]. Restructured genomes with the desired functionalities have served as customized industrial strains that display (i) streamlined metabolic pathways for the production of selected biomaterials, (ii) a reduced production of unwanted by-products, and (iii) increased genome stability [6][7][8][9].
Escherichia coli is the most commonly used microbe for both laboratory research and industrial-scale production of metabolites, such as amino acids and proteins, for therapeutic or commercial uses [10][11][12][13][14]. Because E. coli has adapted to a lifestyle that includes residence in animal intestines with frequent exposure to aqueous and soil environments, many of the genes in its genome are unnecessary for growth in the relatively simple environment within industrial fermentors. Further, the complete genome sequence of E. coli has revealed numerous genes whose products have no known functions and genetic materials that may have been acquired from other organisms in the recent past [15]. In an effort to improve E. coli as an industrial host, many researchers have deleted or added limited numbers of selected genes to the genome or modified plasmids to complement the existing genome [16][17][18][19][20][21][22][23][24]. These efforts have helped researchers make significant progress in improving E. coli as a production host, but have not addressed the productivity problems caused by the numerous E. coli genes with potentially detrimental functions.
Recently, an E. coli genome was reduced by the precise deletion of nonessential genes and other DNA sequencesincluding all known recombinogenic and mobile DNA and cryptic virulence genes-to construct a genetically stable strain that displays robust metabolic performance [5]. The resulting strain, E. coli MDS42, has a chromosome that is 14.3% smaller than that of its parental E. coli strain MG1655. MDS42 shows robust growth under normal laboratory conditions and even better growth in high-cell density fermentations, as well as increased transformation efficiency relative to MG1655 [5]. Therefore, the elimination of unnecessary genes and sequences from an E. coli genome appears to have produced a stable reducedgenome strain without physiological compromise. Furthermore, the deletion of all insertion sequence (IS) ele-ments from the genome means that the strain is free of ISmediated mutagenesis and genomic rearrangements.
In this study, we report the results of a reengineering of MDS42 to increase production of the amino acid L-threonine, which is essential for growth and maintenance of commercial livestock [25]. This reengineering included the overexpression of a feedback-resistant threonine operon (thrA*BC) under the control of a recombinant Tac promoter, deletion of the genes that encode threonine dehydrogenase (tdh) and threonine uptake proteins (tdcC and sstT), and introduction of a mutant threonine exporter gene (rhtA23). The reengineered strain, called MDS-205, shows an ~83% increase in threonine production by flask fermentation relative to the wild-type E. coli strain MG1655 that had been engineered to carry the same threonine-specific modifications. Minimization of an E. coli genome by the elimination of genes unnecessary for growth increases the productivity of the strain by reducing the metabolic burden