 amplification from a T. denticola genomic library, and an open reading frame of 1,629 bp was identified as the coding region for Msp by combining overlapping sequences. The deduced peptide consisted of 543 amino acids and had a molecular mass of 58,233 Da. The peptide had a typical prokaryotic signal sequence with a potential cleavage site for signal peptidase 1. Northern (RNA) blot analysis showing the msp transcript to be approximately 1.7 kb was consistent with the identification of a promoter consensus sequence located optimally upstream of msp and a transcription termination signal found downstream of the stop codon. The entire msp sequence was amplified from T. denticola genomic DNA and cloned in E. coli by using a tightly regulated T7 RNA polymerase vector system. Expression of Msp was toxic to E. coli when the entire msp gene was present. High levels of Msp were produced as inclusion bodies when the putative signal peptide sequence was deleted and replaced by a vector-encoded T7 peptide sequence. Recombinant Msp purified to homogeneity from a clone containing the full-length msp gene adhered to immobilized laminin and fibronectin but not to bovine serum albumin. Attachment of recombinant Msp was decreased in the presence of soluble substrate. Attachment of T. denticola to immobilized laminin and fibronectin was increased by pretreatment of the substrate with recombinant Msp. These studies lend further support to the hypothesis that Msp mediates the extracellular matrix binding activity of T. denticola.Discussion in 'Taxidermy History' started by Ken Edwards, Aug 19, 2008.
By 1999 it was up to 900 visitors.
In 2000 we averaged 1600 visitors.
And now in 2008 we have over 10,000 unique visitors each day, accounting for over 30,000 visits per day, which means most people check the Forum several times per day. We currently serve about 1/4 million page views each day, which is quite a load!
Time flies when you're having fun! Thank you so much for all of the hard work you do for this great forum and for this fine industry. Outstanding job!!! Keep up the great work!!!
Are some of the original members here still active? Just curious who has ridden the storm for the past ten years on here. I have only been here a few months and I have gained so much information and tips that I could have never gotten by just talking to the local taxidermist.
I appreciate everything this website is about. Thanks!!
Its funny, I just put a post up to Yox asking him about the old times. Talk about timing! I cant believe its been 10 yrs.
Congratulations Mr. Edwards, on a job that has gone far and above the "call of duty"...And thank you for providing the taxidermy world a place to come together on a daily basis- it just ROCKS!!! There are many challenges and difficulties that you handle on a daily basis, and there are some within our industry that despise this site its not all fun and games, and for all of that, you deserve a medal!!!!(and maybe a vvveeeeery tall glass of JACK!!! HAPPY TEN TAXI-NET!!! Long may you LIVE!!!
Ken, thank you, once again for this wonderful site. I have been here for only 1 1/2 yrs. and have made many friends. This a wonderful learning tool.
I saw your post on the UTNY topic this morning and it brought back a flood of memories of the early years of the forum. That is when I looked back and discovered that the 10 year anniversary had gone by last week without anyone knowing. Thanks for the reminder!
Ten years, what a milestone in technology for the taxidermy industry. Ken, I can only imagine what the next ten will bring but with you at the helm it's certain to be smooth sailing. Thanks for a great means of communication and education for all of us wether we're just starting out at the bottom or sharing places at the top in taxidermy.
Congratulations Ken. Just think, if you'd have charged a nickel for membership, you'd be rich now. But you're already rich, so what's a few nickels to you anyway??? LOL THANK YOU for this wonderful place to congregate.
Thanks Ken, and congratulations on 10 years.
Thanks Ken, This site has caused me ( at age 65) to learn a hell of allot I did not think I wanted to know. It is fun and brings some things about the industry into perspective. Congrats. on 1- years.
Thank you once again, Ken and WASCO!
Thank's Ken, this forum has helped me immeasurably!! Every time I think I finally know something rock solid about this industry, some great artist comes along to remind me I have a long way to go.
I don't think i've ever shared this part of what the net did for me, but if I have, it bares telling again. Two and a half years ago when I decided to close shop for an extended period & seek help for my drinking problem I made a post being honest about exactly what was going on in my life & why. I left it at that. 2 day's later when I was leaving to go to Houston and admit myself into a rehabilitation facility Miranda handed me a brown envelope and told me to open it when I got there. What it was, is she had printed out all the responses from Taxidermy.net members wishing me the best and writing of some of their own experiences. Every one of them gave me strength, hope & inspiration. I opened that brown envelope many a night when I felt like leaving there and giving up during those 6 months.
I did not have medical insurance so the rehab I went to was not posh to say the least, as a matter of fact it was an old run down apartment complex that still had circa 1960 indoor out door carpet in the rooms with mildew on the walls. We worked on the side of the roads in Houston from 7 A.M. till 6 P.M. every day to pay for what few hours we had left to take clinical classes. It was classified as a (Therapeutic community-GOOGLE it ) so it was tough 24/7.
 I love this industry knew if I straightened my life out I could be an integral part of it & would be wholly accepted if I did what I set out to do. This site and the kind encouraging words from it's members kept me a many of night from packing up my bags and leaving. To state fact, the Taxidermy.net is a major reason I am sober till this day, with out those pages of encouragement, I would not have stayed.
I guess that's one reason when new people come on here and and degrade regular "family" members I take it VERY personally....I have good reason to.
I actually like everybody on this site with the exception of Buck......how could you like a crook ;D ;D LMAO!!
Wow, now I know I am getting old. Congrats Ken and thanks to you and WASCO for starting taxidermy.net. It has come a long ways. I do miss the chats with Yox on the microphone once in a while though! 
Thank you Ken!! I wish I would have been on this site 10 years ago as well as involved with taxidermy.
Michael you are awesome! I also wish I could have been there to encourage you.  You're personality and knowledge makes this page that much more interesting. I hope some day I can meet you in person.
I am just a 31 year old ND guy with quite a bit of knowledge, thanks to this site, and not much experience. I have learned a lot on here and made many friends, even though not meeting anyone just yet. I keep up hope with what little free time and finances I have to someday have my own small taxi business and with this web page, it keeps my hopes up and keeps me kicking to succeed in my goal.
DITTO to everyone! you've all said it so well there's not much left to say except, I love the site and the people on it. We ARE like one big family, we share our hardships and praises, ask for advise, or just come to vent. And someone is always there for us.
I have to blame these Forums for my internet addictions. One of the first sites I ever visited. To this day I come here at LEAST once a day if not more.
Good Job Ken.Monetary policy allows creation of digital/fiat paper. Which is automatically funded by fiscal policy and/or exporting the dollars to overseas banks and governments, which are then the debtor or creditor. The debtor of creditor is using people by any type of labor to fund each currency brought into existance. Firms avoid taxes by all types of ways, but someone has to pay for it. So it always one way or another finds its way to create more debt on the people, in the end of the road  humans always have to pay for the debt. Inflation to fund the debt will just erode the value of currency itself. There is no way governments make money, they just create more debt ontop of debt to fund that debt.🤷😔Hi everyone! Thanks for the great reviews. I'm no longer at Profiles Hair Salon. I'm now at Beautiful Creations 7807 market street boardman ohio 44512. Thanks!
I would highly recommend Jenna. I was very impressed with the knowledge she has about curly hair how to cut it and how to care for it. I have had my share of bad cuts and its refreshing when you can have a stylist who understands exactly